CN104903435A - 用于固定床反应器中的细胞分离的系统和方法 - Google Patents

用于固定床反应器中的细胞分离的系统和方法 Download PDF

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Publication number
CN104903435A
CN104903435A CN201380064957.6A CN201380064957A CN104903435A CN 104903435 A CN104903435 A CN 104903435A CN 201380064957 A CN201380064957 A CN 201380064957A CN 104903435 A CN104903435 A CN 104903435A
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container
fluid
cells
reactor
compartment
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Chinese (zh)
Inventor
苏雷什·普鲁少塔曼
何塞·安东尼奥·卡斯蒂略冈萨雷斯
让-克里斯托弗·德吕格曼
维希沃思·佩泽
德里克·彭德尔伯里
法比安·蒙考贝格
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Pall Atlas Co ltd
Pall Life Science Belgium BVBA
Pall Technology UK Ltd
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Pall Atlas Co ltd
Pall Life Science Belgium BVBA
Pall Technology UK Ltd
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Priority to CN202111185751.1A priority Critical patent/CN114214192A/zh
Publication of CN104903435A publication Critical patent/CN104903435A/zh
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/18—Rollers
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    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201380064957.6A 2012-12-11 2013-12-11 用于固定床反应器中的细胞分离的系统和方法 Pending CN104903435A (zh)

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CN202111185751.1A CN114214192A (zh) 2012-12-11 2013-12-11 用于处理细胞的设备

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US201261735841P 2012-12-11 2012-12-11
US61/735,841 2012-12-11
PCT/US2013/074298 WO2014093439A1 (en) 2012-12-11 2013-12-11 System and method for detachment of cells in fixed bed reactors

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CN201380064887.4A Active CN105229139B (zh) 2012-12-11 2013-12-11 用于细胞培养的器皿
CN202111185751.1A Pending CN114214192A (zh) 2012-12-11 2013-12-11 用于处理细胞的设备

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CN202111185751.1A Pending CN114214192A (zh) 2012-12-11 2013-12-11 用于处理细胞的设备

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US (2) US10781417B2 (https=)
EP (2) EP2931870A4 (https=)
JP (2) JP2016501525A (https=)
CN (3) CN104903435A (https=)
SG (2) SG11201504219QA (https=)
WO (2) WO2014093444A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114761534A (zh) * 2019-11-27 2022-07-15 康宁股份有限公司 固定床细胞培养和收获系统及其使用方法
WO2024093788A1 (zh) * 2022-11-01 2024-05-10 东富龙生命科技有限公司 用于贴壁细胞培养的一次性固定床

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8506797B2 (en) * 2011-04-10 2013-08-13 Therapeutic Proteins International, LLC Downstream bioprocessing device
CN112662611B (zh) * 2014-01-23 2024-10-29 日产化学工业株式会社 培养基组合物
JP6351113B2 (ja) * 2015-01-29 2018-07-04 藤森工業株式会社 振とう型培養装置及びこれを用いた培養方法
US10344257B2 (en) 2015-07-24 2019-07-09 Bioreactor Sciences Llc Horizontally rocked bioreactor system
TWI672375B (zh) * 2015-12-09 2019-09-21 財團法人工業技術研究院 細胞培養載體模組、生物反應器及細胞回收方法
WO2017112455A2 (en) 2015-12-22 2017-06-29 Corning Incorporated Cell separation device and method for using same
CA3021572A1 (en) 2016-04-20 2017-10-26 Centro De Investigaciones Energeticas, Medioambientales Y Tecnologicas Compositions and methods for enhanced gene expression of pklr
WO2018044699A1 (en) 2016-08-27 2018-03-08 3D Biotek, Llc Bioreactor
BE1024733B1 (fr) 2016-11-09 2018-06-14 Univercells Sa Matrice de croissance cellulaire
JP6699837B2 (ja) * 2016-12-27 2020-05-27 株式会社フルステム ３次元多孔性足場からの培養細胞回収方法
US20200115666A1 (en) 2017-03-28 2020-04-16 Corning Incorporated Aseptic bioprocess package
EP3697452A4 (en) 2017-10-16 2021-11-24 Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas, O.A., M.P. LENTIVIRAL VECTORS FOR ADMINISTRATION OF PKLR TO TREAT PYRUVATE KINASE DEFECTS
KR102714703B1 (ko) 2017-12-20 2024-10-07 유니버셀스 테크놀로지스 에스.에이. 생물반응기 및 관련 방법
BR112020020841A2 (pt) 2018-04-11 2021-01-19 Fundacion Para La Investigacion Biomedica Del Hospital Infantil Universitario Nino Jesus Composições e métodos para transplante de célulastronco
IT201800004911A1 (it) * 2018-04-27 2019-10-27 Apparecchiatura per la fermentazione dinamica in stato solido
JP6628448B2 (ja) * 2018-05-29 2020-01-08 藤森工業株式会社 振とう型培養装置及びこれを用いた培養方法
CN108913600B (zh) * 2018-08-16 2023-06-27 杭州德适生物科技有限公司 一种显微授精操作皿
US11692161B2 (en) 2019-02-05 2023-07-04 Corning Incorporated Packed-bed bioreactor systems and methods of using the same
WO2021055934A1 (en) * 2019-09-20 2021-03-25 Pancopia, Inc Animal husbandry nutrient and odor management system
US11118151B2 (en) 2019-11-05 2021-09-14 Corning Incorporated Fixed bed bioreactor and methods of using the same
US12422294B2 (en) 2019-12-31 2025-09-23 Cytiva Us Llc Biocontainer assembly for bioprocessing system
US20220186168A1 (en) * 2020-12-11 2022-06-16 Cell Spinpod LLC Rotating suspension culture devices that allow direct microscopy, in situ assays, and automation
US20240279598A1 (en) * 2021-06-03 2024-08-22 Univercells Technologies Sa Bioreactor system with enhanced cell harvesting capabilities and related methods
CN114456938A (zh) * 2022-03-02 2022-05-10 蓝莲(杭州)生物科技有限公司 滚动式细胞培养仪
WO2023249816A1 (en) * 2022-06-22 2023-12-28 Corning Incorporated Fixed bed cell culture reactor vessel for substrate alignment and sampling
EP4619499A2 (en) * 2022-11-18 2025-09-24 Univercells Technologies SA Systems and methods for recovering target biomolecules from fixed bed bioreactors
WO2025049667A1 (en) * 2023-08-31 2025-03-06 Corning Incorporated Perfusion bioreactors and systems for large scale cell culture
TWM664031U (zh) * 2024-01-22 2024-12-01 覺之能量研究有限公司 細胞培養輔助器
WO2026012929A1 (en) * 2024-07-07 2026-01-15 Univercells Technologies Sa Apparatus for cell culturing and related methods
CN119776116B (zh) * 2025-03-10 2025-05-09 湖南中瑞互信医疗科技有限公司 一种恒温孵育摇床装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4322296A (en) * 1980-08-12 1982-03-30 Kansas State Univ. Research Foundation Method for wastewater treatment in fluidized bed biological reactors
CN1750868A (zh) * 2003-02-13 2006-03-22 Ilc多弗有限公司 柔性可任意处理的容器
CN101316925A (zh) * 2005-10-04 2008-12-03 阿尔特莱斯公司 细胞培养方法以及进行该方法的设备
US20110281343A1 (en) * 2008-10-02 2011-11-17 Gay Roger J Bioreactor with rods arrayed for culturing anchorage-dependent cells

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4253947A (en) * 1979-02-12 1981-03-03 Kansas State University Research Foundation Method for wastewater treatment in fluidized bed biological reactors
US5266476A (en) 1985-06-18 1993-11-30 Yeda Research & Development Co., Ltd. Fibrous matrix for in vitro cell cultivation
US4720462A (en) 1985-11-05 1988-01-19 Robert Rosenson Culture system for the culture of solid tissue masses and method of using the same
JPH02109973A (ja) * 1988-10-19 1990-04-23 Shimadzu Corp 細胞培養装置
JPH02177882A (ja) * 1988-12-29 1990-07-10 Shimadzu Corp 細胞培養装置用レベルセンサ
JPH0728722B2 (ja) * 1990-06-29 1995-04-05 富士写真フイルム株式会社 バイオリアクター装置
WO1992007615A1 (en) * 1990-10-29 1992-05-14 Regents Of The University Of Minnesota A bioartificial liver
BE1004610A5 (fr) 1990-11-29 1992-12-22 Spielmann Richard Bioreacteur rotatif a grande surface pour culture de cellules adherentes ou en suspension.
FR2690926B1 (fr) 1992-05-06 1995-08-04 Lvmh Rech Dispositif du type reacteur a volume variable et procede de culture cellulaire.
IL108321A (en) 1994-01-12 1998-10-30 Yeda Res & Dev Bioreactor and system for improved productivity of photosynthetic algae
US5958761A (en) 1994-01-12 1999-09-28 Yeda Research And Developement Co. Ltd. Bioreactor and system for improved productivity of photosynthetic algae
US5527705A (en) 1994-09-12 1996-06-18 Becton, Dickinson And Company Roller bottle for trans-membrane co-culture of cells and method for its use
JP3639007B2 (ja) 1995-08-22 2005-04-13 京都水研株式会社 攪拌槽型のバイオリアクタ
GB9803362D0 (en) * 1998-02-17 1998-04-15 Cellon Sa A cell culture vessel
JP2000125848A (ja) * 1998-10-19 2000-05-09 Agriculture Forestry & Fisheries Technical Information Society 細胞培養用具及び前記細胞培養用具を用いる簡易細胞培養方法
WO2001055293A2 (en) * 2000-01-25 2001-08-02 The Victoria University Of Manchester Delayed release of fluids
US6720178B1 (en) * 2000-06-29 2004-04-13 University Of Louisville Research Foundation, Inc. Self-feeding roller bottle
US20020155594A1 (en) 2001-03-27 2002-10-24 Hsieh Helen V. Method and apparatus for culturing cells
KR20020088848A (ko) 2001-05-21 2002-11-29 (주)코아바이오텍 세포배양관 및 이를 이용한 대량 세포배양기
WO2003008931A2 (en) * 2001-07-17 2003-01-30 Georgi Hvichia Microstructure for particle and cell separation, identification, sorting, and manipulation
TWI242600B (en) * 2001-11-09 2005-11-01 Ind Tech Res Inst Collection, culture device and method for drawing out cell suspension from cell supply source
JP2004041093A (ja) * 2002-07-12 2004-02-12 Mitsubishi Heavy Ind Ltd 細胞培養装置および細胞培養方法
CA2493477A1 (en) * 2002-07-31 2004-02-05 Japan Science And Technology Agency Automatic culture apparatus for cell or tissue with biological origin
JP4242618B2 (ja) * 2002-09-03 2009-03-25 オリンパス株式会社 培養装置
JP2004089136A (ja) * 2002-09-03 2004-03-25 Olympus Corp 培養容器
CN1300297C (zh) * 2002-09-20 2007-02-14 华东理工大学 一种用于动物细胞扩大接种的消化反应器
US7390653B2 (en) 2002-12-04 2008-06-24 Synthecon, Inc. Culture chamber for biologicals
DE10322054B4 (de) * 2003-05-15 2015-06-18 Sartorius Stedim Biotech Gmbh Vorrichtung und Verfahren zur Kultivierung von Zellen
EP1692257B1 (en) 2003-11-10 2013-04-24 Wheaton Industries, Inc. Compartmentalized device for cell culture, cell processing, and sample dialysis
EP1739164B1 (en) 2004-04-13 2016-08-10 Toyo Seikan Group Holdings, Ltd. Double incubator and incubating method
DE102004062828B4 (de) 2004-12-27 2007-01-11 Fresenius Medical Care Deutschland Gmbh Reaktor mit einer rotierbar angeordneten Reaktoreinheit
CN1740314A (zh) * 2005-07-21 2006-03-01 高春平 持续灌注式自动细胞培养系统
US7856704B2 (en) * 2005-10-14 2010-12-28 Gm Global Technology Operations, Inc. Monitoring system for clinching process
JP2007222064A (ja) * 2006-02-23 2007-09-06 Scimedia Ltd ホローファイバー型培養装置
DE102006044795A1 (de) * 2006-09-13 2008-03-27 Schweigert, Florian, Prof. Dr.med.vet. Bestimmung von Inhaltsstoffen aus biologischen Materialien
WO2008136371A1 (ja) 2007-04-27 2008-11-13 Toyo Seikan Kaisha, Ltd. 細胞培養装置、細胞培養システム及び細胞培養方法
WO2009139703A1 (en) * 2008-05-15 2009-11-19 Ge Healthcare Bio-Sciences Ab Method for cell expansion
DE102008039812A1 (de) 2008-08-25 2010-03-04 Yvonne Ibold Vorrichtung und Verfahren zur Kultivierung von adhärenten Zellen
FR2943560B1 (fr) * 2009-03-24 2011-05-27 Jean Pascal Zambaux Bioreacteur jetable et systeme d'agitation a usage unique
CN102108333B (zh) * 2009-12-29 2014-08-13 上海日泰医药设备工程有限公司 一种流化床生物反应器
JP2013533171A (ja) * 2010-04-19 2013-08-22 エイティーエムアイ ビーブイビーエイ 流体処理および貯蔵容器用のドレンコネクタ
JP5947790B2 (ja) * 2010-05-25 2016-07-06 クック・バイオテック・インコーポレイテッドＣｏｏｋ Ｂｉｏｔｅｃｈ Ｉｎｃｏｒｐｏｒａｔｅｄ 医療用移植片の細胞播種に有用な方法、基質、およびシステム
JP2014515605A (ja) * 2011-03-28 2014-07-03 コーニング インコーポレイテッド 耐キンク性コネクタを備えた容器蓋
SG10201601537QA (en) * 2011-04-15 2016-04-28 Pluristem Ltd Methods and systems for harvesting cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4322296A (en) * 1980-08-12 1982-03-30 Kansas State Univ. Research Foundation Method for wastewater treatment in fluidized bed biological reactors
CN1750868A (zh) * 2003-02-13 2006-03-22 Ilc多弗有限公司 柔性可任意处理的容器
CN101316925A (zh) * 2005-10-04 2008-12-03 阿尔特莱斯公司 细胞培养方法以及进行该方法的设备
US20110281343A1 (en) * 2008-10-02 2011-11-17 Gay Roger J Bioreactor with rods arrayed for culturing anchorage-dependent cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
梁学礼: "《植物遗传学 下》", 31 December 1982 *
章静波等: "《细胞生物学实验技术》", 31 August 2011 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114761534A (zh) * 2019-11-27 2022-07-15 康宁股份有限公司 固定床细胞培养和收获系统及其使用方法
US12534698B2 (en) 2019-11-27 2026-01-27 Corning Incorporated Fixed bed cell culture and harvesting system and methods of using the same
WO2024093788A1 (zh) * 2022-11-01 2024-05-10 东富龙生命科技有限公司 用于贴壁细胞培养的一次性固定床

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