CN104823722A - Method for relieving flooding stress of cucumbers - Google Patents
Method for relieving flooding stress of cucumbers Download PDFInfo
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- CN104823722A CN104823722A CN201410046184.5A CN201410046184A CN104823722A CN 104823722 A CN104823722 A CN 104823722A CN 201410046184 A CN201410046184 A CN 201410046184A CN 104823722 A CN104823722 A CN 104823722A
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- cucumber
- grafting
- waterflooding
- black seed
- root
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Abstract
The invention discloses a method for relieving flooding stress of cucumbers. The method is characterized in that the cucumbers are grown under a root flooding condition, which is realized through a method of grafting roots of cucurbita ficifolia. The adopted cucumber variety is Spring-Autumn King provided by the Protected Horticulture Institute of Shanghai Academy of Agricultural Sciences, and the adopted cucurbita ficifolia variety is Heiyu I. By means of grafting the roots of cucurbita ficifolia, the problem that the cucumbers are relatively low in flooding resistance is effectively solved.
Description
Technical field
The present invention relates to the culture technique improving the resistance to waterflooding of cucumber, specifically a kind of culture technique being improved the resistance to waterflooding of cucumber by the grafting of black seed pumpkin root.
Background technology
China is that more country occurs in flood, especially south waterflooding in summer threatens very large to the good quality and high output of agricultural production, vegetable crop, in addition a lot of Soils In The Region is sticky heavy, increases waterflooding further to producing the loss caused, and does not still have good mitigation strategy at present.Cucumber (Cucumis sativus L.) originates from subtropical zone, and be one of vegetables of China's cultivation, belong to shallow root system plant, it grows and requires better soil aeration condition.Therefore need one namely can improve the resistance to waterflooding of cucumber, again a kind of effective ways alleviated the injury of crop waterflooding adverse circumstance, adapt to adverse circumstance and raising stress resistance, for the improvement of cucumber cultivation technology and resistance breeding provide theoretical foundation, enrich plant stress physiology theoretical simultaneously.And black seed pumpkin well developed root system, root elongation ability is strong, is suitable for the stock doing grafting.
Summary of the invention
The present invention proposes a kind of method that method by the grafting of black seed pumpkin root improves the resistance to waterflooding of cucumber.The present invention is achieved in that a kind of by the resistance to waterflooding cultivation method of black seed pumpkin root grafting raising cucumber, it is characterized in that: described resistance to waterflooding cucumber be through the grafting of black seed pumpkin root method realize, under the process of root flooding condition, black seed pumpkin grafting has certain mitigation to the cucumber under flooding stress." king in spring and autumn " that cucumber variety provides for Shanghai Academy of Agricultural Sciences; Black seed pumpkin kind is " No. one, jet ".Planting form used is potted plant, and flooding condition is just to cross soil table 1cm for standard, and Submergence time is 3 days, effectively solves the poor problem of the resistance to waterflooding of cucumber by black seed pumpkin root grafting means;
The invention has the beneficial effects as follows: the impact report of black seed pumpkin root grafting on cucumber physiological property under flooding stress is less, by the method for black seed pumpkin root grafting, outside the resistance to waterflooding of effective raising cucumber, to providing theories integration for the High efficiency and high quality cultivation of cucumber and sustainable development; And by research grafting to the various Physiological Mechanism in Regulation of plant under flooding stress, disclose the function and effect mechanism of grafting in plant, the effect of grafting in plant abiotic is coerced can be furtherd investigate further.
Accompanying drawing explanation
Fig. 1 is mitigation (note: CS, normal condition under the grafting green cucumber cucumber of grafting to cucumber Net Photosynthetic Rate under flooding condition; CF, black seed pumpkin Grafted Cucumber Seedling under normal condition; CSt, grafting green cucumber cucumber under flooding condition; CFt, black seed pumpkin Grafted Cucumber Seedling under flooding condition.）
Embodiment:
1. the cultivation method of the resistance to waterflooding of this raising cucumber in greenhouse is realized by black seed pumpkin root graft technology.The conditions such as the process after the time of grafting, the method for grafting, grafting should be taken into full account, will control time of submerging treatment simultaneously well;
This patent pumpkin used is " No. one, jet ".Grafting is selected when cucumber cotyledons flattens, and will note heat and moisture preserving after grafting.By the grafting of research black seed pumpkin root to the various Physiological Mechanism in Regulation of cucumber under flooding stress, disclose the function and effect mechanism of grafting in plant further; And improving the resistance to waterflooding of cucumber by grafting, method is simple.
2. test medical record
Cucumber seeds and black seed pumpkin seed can vernalization simultaneously, so enter to sow respectively afterwards, and black seed pumpkin sprouts and emerges comparatively slow, treats that its cotyledon launches, cucumber cotyledons also flattened also a certain size, at this moment can carry out grafting;
First black seed pumpkin bud is removed with clean blade; again from central vertical incision about 1cm; rapidly by 1.5-2cm incision wedgewise under cucumber cotyledons; wedge length is about 1cm also, in the stem mouth that insertion black seed pumpkin cuts afterwards, and grafting clip on folder; grafting is placed in Small plastic shed or the incubator of humidification; in high humidity environment, grow about 3 days, after grafting survives, progressively throw off canopy film;
Submerging treatment is carry out in the benzvalene form container of 0.9m*0.55m*0.25m at the wide * height of long *, waterflooding is just to cross soil table 1cm for standard, Submergence time is 3 days, test cucumber growth is in the plastic flowerpot of 20cm*20cm, matrix used is rural area Nutrition Soil, when seedling 6 leaf 1 heart (the first tenday period of a month in May, 2012), start root waterflooding test.
Embodiment
black seed pumpkin grafting is on the impact of flooding stress cucumber Net Photosynthetic Rate
1 materials and methods
1.1 material
For examination cucumber (
cucumis sativus L.) be European mini cucumber " king in spring and autumn ", provided by Academy of Agricultural Sciences, Shanghai City.Black seed pumpkin is " No. one, jet ".
1.2 method
1.2.1 examination material is cultivated
To cucumber, black seed pumpkin seed presoaking and germinating, be sowed in 50 hole dishes afterwards, the grafting of black seed pumpkin root is carried out when cucumber cotyledons just flattens when black seed pumpkin cotyledon launches, transplant after grafting in the plastic flowerpot of 20cm*20cm, nursery and transplanting medium are rural area Nutrition Soil, when seedling 6 leaf 1 heart (the first tenday period of a month in May, 2012), start root waterflooding test, waterflooding is just to cross soil table 1cm for standard, and each process 6 strain, repeats 3 times.
Test arranges 4 process: contrast, grafting green cucumber cucumber under normal condition; Black seed pumpkin Grafted Cucumber Seedling under normal condition; Grafting green cucumber cucumber under flooding condition; Black seed pumpkin Grafted Cucumber Seedling under flooding condition.Transplant in the plastic flowerpot of 20cm* 20cm after launching respectively at the 1st true leaf, nursery and transplanting medium are rural area Nutrition Soil, treat young plant 6 leaf 1 heart, the basically identical young plant of size is selected to carry out waterflooding test (in mid-May, 2012), waterflooding is just to cross soil table 1cm for standard, each process 6 strain, repeats 3 times.Each process waterflooding started to recover after 3 days.
1.2.2 the mensuration of Gas exchange parameters
Utilize the photosynthetic instrument (LI-6400 of LI-6400 type; LI-COR, Inc., Lincoln, NE, U.S.) measurement that Gas exchange parameters Net Photosynthetic Rate (Pn) is carried out, the light intensity of measurement is all set to 600 μm of olm
-2s
-1, all process replications 5 times, result represents with mean value ± standard error.
2. result
The change of different disposal Net Photosynthetic Rate after 2.1 waterflooding
As seen from Figure 1, waterflooding is after 1 day, and each process Net Photosynthetic Rate difference is not obvious, but waterflooding after 3 days waterflooding plant Net Photosynthetic Rate reduce clearly, but the Amplitude Ratio cucumber that the plant of black seed pumpkin grafting process reduces is little from the plant of root graft; Waterflooding is after 5 days, and waterflooding respectively processes Net Photosynthetic Rate and reduces further, and the Net Photosynthetic Rate of black seed pumpkin grafting is still higher than cucumber from root graft plant; Recover 3 days after waterflooding, although each process recovery effects is all not obvious, the Net Photosynthetic Rate of black seed pumpkin grafting is still higher than cucumber from root graft seedling.
Claims (3)
1. the cultivation method utilizing grafting to improve the resistance to waterflooding of cucumber, it is characterized in that: described resistance to waterflooding cucumber be through the grafting of black seed pumpkin root method realize, under the process of root flooding condition, black seed pumpkin grafting has certain mitigation to the cucumber under flooding stress.
2. according to the cultivation method of the resistance to waterflooding cucumber described in claim 1, it is characterized in that: flatten in cucumber cotyledons, when black seed pumpkin cotyledon launches, carry out root grafting with black seed pumpkin, adopt suitable heat and moisture preserving.
3. the cultivation method of the resistance to waterflooding cucumber according to claim 1 and 2, it is characterized in that: cucumber used is root waterflooding, waterflooding is just to cross soil table 1cm for standard, and Submergence time is 3 days, and the black seed pumpkin selected by grafting only uses in cucumber " king in spring and autumn ".
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410046184.5A CN104823722A (en) | 2014-02-10 | 2014-02-10 | Method for relieving flooding stress of cucumbers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410046184.5A CN104823722A (en) | 2014-02-10 | 2014-02-10 | Method for relieving flooding stress of cucumbers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104823722A true CN104823722A (en) | 2015-08-12 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410046184.5A Pending CN104823722A (en) | 2014-02-10 | 2014-02-10 | Method for relieving flooding stress of cucumbers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104823722A (en) |
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2014
- 2014-02-10 CN CN201410046184.5A patent/CN104823722A/en active Pending
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150812 |