CN104823629B - The method that Southern Pine high temperature season cuttings promotees callus - Google Patents
The method that Southern Pine high temperature season cuttings promotees callus Download PDFInfo
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- CN104823629B CN104823629B CN201510175430.1A CN201510175430A CN104823629B CN 104823629 B CN104823629 B CN 104823629B CN 201510175430 A CN201510175430 A CN 201510175430A CN 104823629 B CN104823629 B CN 104823629B
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Abstract
The invention discloses a kind of method that Southern Pine high temperature season cuttings promotees callus, by artificially reducing portion temperature in cuttings, makes it below cuttings lower part temperature, promotes cuttings to heal in advance.Experiment shows, is pre-processed before carrying out pine tree cuttage using the present invention, forms within 20-25 days callus, and the time taken root from callus induction is 20 days or so, and cuttings added up rootage duration as 43-46 days, shortens 14-17 days than conventional method.Meanwhile the band leaf portion in low temperature environment divides physiological activity slow, water demand and susceptible gene are decreased obviously, and are greatlyd save and are managed and protected cost.Method economical and efficient and operation of the invention is simple, solves pine tree high temperature season cuttage and manages and protects the problem of difficulty is big, and survival rate is low, promotes and applies suitable for the production unit of southern conifer cutting propagation, has important practical significance to Southern Pine scale cutting propagation.
Description
Technical field
The invention belongs to Southern Pine cutting propagation field, more particularly to a kind of Southern Pine high temperature season cuttings to promote the side of callus
Method.
Background technology
The Southern Pines such as masson pine, wet-land pine tree and wet plus pine are the important dual-purpose seeds of fat material of south China, widely used, warp
Ji value is high, is the primary raw material seeds of the industries such as China's woods slurry paper (plate), rosin and building.Except timber and two, rosin are large
Outside purposes, masson pine full tree it is available, pollen, needle and bark be rich in antioxidant, can be used as excellent anti-aging with
Antioxidant health-care product；Pine nut oil-containing 30%, can manufacture paint, soap and lubricating oil；Cone can refine crude oil；Pine stump can extract pine
Tar, moreover it is possible to cultivate Chinese medicine --- the Poria cocos of preciousness.
Studied by the genetic improvements of more than 30 years, a collection of excellent masson pine of Guangxi selection and breeding, wet-land pine tree and wet plus pine
Superior families and clone.Due to the reproductive physiology feature of seeds in itself, the vegetative propagation of coniferous species is more difficult than deciduous species,
It is most difficult to again with the vegetative propagation of conifer seeds in coniferous species.The difficult point of conifer vegetative propagation mainly has three：First, cuttings has
There is obvious age effect, the age of tree was difficult to root induction more than 5 years；Second, difficulty of taking root；Third, rootage duration is grown, above feature
Develop slowly the vegetative propagation of pine tree.During the nearly last ten years, vegetative propagation of many scholars to pine tree has carried out going deep into system
The cutting propagation of the southern conifer seeds such as research, masson pine and wet plus pine obtains certain progress, Guangdong and Guangxi etc. a few
R＆D institution has possessed the ability of large-scale production pine tree cuttage seeding, but the cutting propagation time it is long, it is of high cost be still loose at this stage
Tree cutting propagation needs the wide gap crossed over.
In theory, the higher healing for being more advantageous to cuttings wound of temperature, but managed very after hot and humid condition underthrust
Difficulty, the reason is that then needle transpiration rate is big, it is necessary to moisture is replenished in time for temperature height, but hot and humid easily grow germ and leads
Survival rate is caused significantly to decline.Therefore, summer cuttage is always the technical bottleneck of pine tree cutting propagation.In south China osmanthus Guangdong etc.
Area, the max. daily temperature in 7-October is up to 28-35 DEG C, and temperature is high and the daytime day temperature difference is small, when pine tree cuttage needs to avoid this section
Between, although the reason is that this 4 months cuttage rootage durations are short, slotting difficult management, survival rate afterwards are low.Therefore, skewer under hot conditions
Intubation reason is pine tree cutting propagation important technological problems urgently to be resolved hurrily.
The content of the invention
Simple, economical and efficient Southern Pine high temperature season cuttings is operated the technical problem to be solved in the present invention is to provide a kind of
Promote the method for callus, to improve the survival rate of Southern Pine high temperature season cuttage and cost is managed and protected in reduction.
In order to solve the above technical problems, the present invention uses following technical scheme：Southern Pine high temperature season cuttings promotees callus
Method, it is artificial to manufacture cuttings top and the bottom temperature difference, promote cuttings to heal in advance.
The method that above-mentioned Southern Pine high temperature season cuttings promotees callus, by artificially reducing cuttings (band leaf portion point) temperature, makes
It is less than cuttings (notch) temperature.
The method that above-mentioned Southern Pine high temperature season cuttings promotees callus, light ground mass is inserted in by pine tree cuttings is close in high temperature season
In container, with black thin film sealing cuttings band leaf portion point, cuttage vessel is stood upside down be positioned in horizontal chest freezer carry out promoting callus it is pre-
Processing, makes cuttings notch be placed in outside horizontal chest freezer, and cuttings (band leaf portion point) is placed in refrigerator-freezer.
Southern Pine is masson pine, wet-land pine tree or wet plus loose
Promote callus to pre-process 20-25 days, during which temperature is set to 7-8 DEG C in horizontal chest freezer.
The method that above-mentioned Southern Pine high temperature season cuttings promotees callus, comprises the following steps：
<1>Freezer door is transformed --- the glass door of horizontal chest freezer is cut out into several strips by the size of cuttage vessel,
Container edge is just stuck in glass for refrigerator outdoors after making cuttage vessel inversion；
<2>Cuttage vessel --- the transparent disposable plastic box of selection 15cm × 10cm × 5cm, with puncher in plastic casing
1 is punched every 5cm or so in five faces in addition to top surface, moistening coconut palm chaff compacting will be filled in container, is sealed container with mulch
Mouthful；
<3>Cuttage --- in the high temperature season in southern 7-October, semi-wooden scion is chosen, cuts flat notch, clear water immersion
10-12h removes rosin, soaks 1-3h with the IBA solution of 200-500ppm of concentration, and then carbendazim soaks 5-10min, will insert
Fringe is inserted into step<2>In ready cuttage vessel, spray and squirt cuttings needle, black thin film sealing cuttings；
<4>Cuttings is inverted --- by step<3>The good cuttings of middle cuttage, which is stood upside down, is positioned over step<1>Improved refrigerator-freezer
In, cuttings band leaf portion is placed in refrigerator-freezer, cuttings notch is placed in outside refrigerator-freezer；
<5>Management --- the moisture change of coconut palm chaff in observation cuttage vessel, coconut palm chaff color during brown stain yellowly by using
Spraying keeps the skin wet from the aperture of container edge；
<6>Crop field cuttage --- by step<4>With<5>Pretreatment 20-25 days, treats that cuttings notch expands, callus
Tissue induction is completed, and moves into nutrition cup cuttings carry out conventional culture of rootage at this time.
Step<3>In cuttage depth be 4-4.5cm.
For the problems such as Southern Pine high temperature season cuttage survival rate is low, management cost is high, inventor is ingenious using in cuttings
The lower end temperature difference is beneficial to the characteristics of healing, establishes a kind of method that Southern Pine high temperature season cuttings promotees callus, i.e., in high temperature season
Insert in pine tree cuttings is close in light ground mass container, with black thin film sealing cuttings band leaf portion point, cuttage vessel is stood upside down and is placed
Carry out promoting callus pretreatment in horizontal chest freezer, cuttings notch is placed in outside horizontal chest freezer, cuttings (band leaf portion point) is placed in refrigerator-freezer
It is interior, by artificially reducing cuttings band leaf portion temperature, cuttings Incision temperature is made it below, promotes cuttings to heal in advance.Meanwhile locate
Divide physiological activity slow in the band leaf portion of low temperature environment, water demand and susceptible gene are decreased obviously, and are greatlyd save and are managed and protected cost.
Experiment shows, is pre-processed before carrying out pine tree cuttage using the present invention, forms callus within 20-25 days, is given birth to from callus induction
The time of root is 20 days or so, and cuttings added up rootage duration as 43-46 days, shortens 14-17 days than conventional method.The present invention's
Method economical and efficient and operation is simple, solves Southern Pine high temperature season cuttage and manages and protects the problem of difficulty is big, and survival rate is low, be suitable for
The production unit of southern conifer cutting propagation promotes and applies, and has important practical significance to Southern Pine scale cutting propagation.
Brief description of the drawings
Fig. 1 is the method schematic diagram that Southern Pine high temperature season cuttings of the present invention promotees callus.
In figure：1 cuttage vessel, 2 pine tree cuttings, 3 black thin films, 4 strip shape gobs, 5 glass doors, 6 horizontal chest freezers.
Embodiment
Embodiment 1
<1>Freezer door is transformed --- the glass door (100cm × 60cm) of horizontal chest freezer cut by the size of cuttage vessel
Go out 3 strip shape gobs (100cm × 9.5cm), container edge is just stuck in glass for refrigerator outdoors after making cuttage vessel inversion；It will cut out
3 glass bars install additional aluminium alloy, you can reduce freezer door when being induced without callus；
<2>Cuttage vessel --- the transparent disposable plastic box of selection 15cm × 10cm × 5cm, with puncher in plastic casing
1 (3 hole of long face, wide 2 hole of face, high 1 hole of face, totally 12 hole) is punched every 5cm or so in five faces in addition to top surface, to breathe freely
With keep the skin wet, moistening coconut palm chaff compacting will be filled in container, with mulch by container closure；
<3>Cuttage --- on July 5th, 2014, semi-lignified cutting ofPinus massoniana is chosen, cuts flat notch, clear water immersion 12h
Rosin is removed, soaks 3h with the IBA solution of concentration 200ppm, then 0.5% carbendazim immersion 10min, step is inserted into by cuttings<2>
In ready cuttage vessel, cuttage 55 in 1 plastic casing, cuttage depth is 4cm (it is required that 1 cuttage success, repeatedly cuttage
Cuttings can not fix when loosening matrix and causing to be inverted), spraying squirts cuttings needle, and black thin film internal layer is sprayed last layer
Wet fog covers cuttings after (being advisable with not dripping), and black thin film and cuttings interval 5cm, black thin film sack are fixed with rubber band
In plastics box mouth；
<4>Cuttings (cuttage vessel) is inverted --- by step<3>The good cuttings of middle cuttage (cuttage vessel), which is stood upside down, to be positioned over
Step<1>Carry out promoting callus pretreatment in improved refrigerator-freezer, cuttings band leaf portion is placed in refrigerator-freezer, container is placed in refrigerator-freezer
Outside；
<5>Management --- the moisture change of coconut palm chaff in observation cuttage vessel, coconut palm chaff color during brown stain yellowly by using
Spraying keeps the skin wet from the aperture of container edge；
<6>Crop field cuttage --- by step<4>With<5>Pre-process 25 days (temperature is set to 7 DEG C in period horizontal chest freezer),
Cuttings notch expands, and callus induction is completed, and moves into nutrition cup cuttings carry out conventional culture of rootage at this time.
Embodiment 2
<1>With embodiment 1
<2>With embodiment 1
<3>Cuttage --- in August in 2014 5 days, semi-lignified wet-land pine tree cuttings is chosen, cuts flat notch, clear water immersion 10h
Rosin is removed, soaks 1h with the IBA solution of concentration 500ppm, then 0.3% carbendazim immersion 8min, step is inserted into by cuttings<2>
In ready cuttage vessel, cuttage 60 in 1 plastic casing, cuttage depth is 4.5cm (it is required that 1 cuttage success, repeatedly skewer
Inserting cuttings when loosening matrix and causing to be inverted can not fix), spraying squirts cuttings needle, and black thin film internal layer is sprayed one
Cuttings is covered after layer wet fog (being advisable with not dripping), black thin film and cuttings interval 10cm, black thin film sack rubber band
It is fixed on plastics box mouth；
<4>With embodiment 1
<5>With embodiment 1
<6>Crop field cuttage --- by step<4>With<5>Pre-process 23 days (temperature is set to 8 DEG C in period horizontal chest freezer),
Cuttings notch expands, and callus induction is completed, and moves into nutrition cup cuttings carry out conventional culture of rootage at this time.
Embodiment 3
<1>With embodiment 1
<2>With embodiment 1
<3>Cuttage --- in September in 2014 3 days, semi-lignified wet-land pine tree cuttings is chosen, cuts flat notch, clear water immersion 12h
Rosin is removed, soaks 2h with the IBA solution of concentration 300ppm, then 0.1% carbendazim immersion 10min, step is inserted into by cuttings<2>
In ready cuttage vessel, cuttage 65 in 1 plastic casing, cuttage depth is 4cm (it is required that 1 cuttage success, repeatedly cuttage
Cuttings can not fix when loosening matrix and causing to be inverted), spraying squirts cuttings needle, and black thin film internal layer is sprayed last layer
Wet fog covers cuttings after (being advisable with not dripping), and black thin film and cuttings interval 10cm, black thin film sack are consolidated with rubber band
Due to plastics box mouth；
<4>With embodiment 1
<5>With embodiment 1
<6>Crop field cuttage --- by step<4>With<5>Pre-process 21 days (temperature is set to 7 DEG C in period horizontal chest freezer),
Cuttings notch expands, and callus induction is completed, and moves into nutrition cup cuttings carry out conventional culture of rootage at this time.
Embodiment 4
<1>With embodiment 1
<2>With embodiment 1
<3>Cuttage --- on October 5th, 2014, selection semi-lignified is wet to add loose cuttings, cuts flat notch, clear water immersion
11h removes rosin, soaks 2.5h with the IBA solution of concentration 400ppm, then 0.3% carbendazim immersion 10min, step is inserted into by cuttings
Suddenly<2>In ready cuttage vessel, cuttage 50 in 1 plastic casing, cuttage depth for 4.5cm (it is required that 1 cuttage success,
Cuttings can not fix when cuttage loosens matrix and causes to be inverted repeatedly), spraying squirts cuttings needle, by black thin film internal layer
Cuttings is covered after spray last layer wet fog (being advisable with not dripping), black thin film and cuttings interval 8cm, black thin film sack use rubber
Rubber band is fixed on plastics box mouth；
<4>With embodiment 1
<5>With embodiment 1
<6>Crop field cuttage --- by step<4>With<5>Pre-process 25 days (temperature is set to 8 DEG C in period horizontal chest freezer),
Cuttings notch expands, and callus induction is completed, and moves into nutrition cup cuttings carry out conventional culture of rootage at this time.
Reference examples
Cuttage carries out in greenhouse, greenhouse area 300m2, surrounding covering light transmittance be 90% plastic film, upper and lower two
75% shading net of layer, shading net and film can manual control openings.On August 6th, 2014, choose masson pine, wet-land pine tree and it is wet plus
Loose semi-wooden scion totally 500, cuts flat notch, IBA 300ppm immersion 2h, 0.1% carbendazim immersion 10min, and cuttage is to wet
In the coconut palm chaff matrix of profit.The folding of shading net and film and the moisture of cuttings is controlled to supplement by Hygrothermograph in greenhouse, temperature
Canopy internal spraying is opened when degree is less than 75% more than 33 DEG C with humidity and is cooled down and increased humidity.On October 5th, 2014 counts, its
Middle cuttings preserves 185, takes root 111, storage rate 37.0%, takes root because being dehydrated, rotting and dead 315 of the reason such as susceptible
Rate 33.0%.
1 callus inducing effect comparison sheet of table
| Callus induction rate (%) | Cuttage survival rate (%) | Rootage duration (my god) | |
| Embodiment 1 | 93.6 | 87.6 | 46 |
| Embodiment 2 | 95.3 | 85.3 | 43 |
| Embodiment 3 | 90.5 | 84.7 | 45 |
| Embodiment 4 | 97.7 | 89.5 | 43 |
| Reference examples | 33.0 | 60 |
Conclusion：As seen from Table 1, the embodiment 1~4 handled using the present invention, Callus induction rate are 90.5~97.7%, skewer
Motility rate is inserted into as 84.7~89.5%；And in reference examples, cuttage survival rate is only 33.0%, the cuttages of embodiment and reference examples into
Motility rate differs greatly.The rootage duration of embodiment 1~4 is 43-46 days, hence it is evident that better than 60 days of reference examples, rootage duration shortened
15 days or so, have a clear superiority.Simultaneously as the shortening of cuttage root-taking time, has saved and managed and protected cost, cuttage is improved
Reproductive efficiency.
Claims (2)
1. a kind of method that Southern Pine high temperature season cuttings promotees callus, it is characterised in that：It is in high temperature season that Southern Pine cuttings is close
Insert in light ground mass container, with black thin film sealing cuttings band leaf portion point, cuttage vessel is stood upside down and is positioned in horizontal chest freezer
Carry out promoting callus pretreatment, cuttings notch is placed in outside horizontal chest freezer, cuttings top is placed in refrigerator-freezer；The rush callus pretreatment
20-25 days, during which temperature was set to 7-8 DEG C in horizontal chest freezer；Specifically operation carries out the method according to the following steps：
<1>Freezer door is transformed --- and the glass door of horizontal chest freezer is cut out into several strips by the size of cuttage vessel, makes skewer
Container edge is just stuck in glass for refrigerator outdoors after inserting container upside down；
<2>Cuttage vessel --- the transparent disposable plastic box of selection 15cm × 10cm × 5cm, top is removed with puncher in plastic casing
1 is punched every 5cm or so in five faces outside face, moistening coconut palm chaff compacting will be filled in container, with mulch by container closure；
<3>Cuttage --- in the high temperature season in southern 7-October, semi-wooden scion is chosen, cuts flat notch, clear water immersion 10-
12h removes rosin, soaks 1-3h with the IBA solution of 200-500ppm of concentration, then carbendazim soaks 5-10min, and cuttings is inserted
To step<2>In ready cuttage vessel, spray and squirt cuttings needle, black thin film sealing cuttings band leaf portion point；
<4>Cuttings is inverted --- by step<3>The good cuttings of middle cuttage, which is stood upside down, is positioned over step<1>In improved refrigerator-freezer, make
Cuttings band leaf portion is placed in refrigerator-freezer, and cuttings notch is placed in outside refrigerator-freezer；
<5>Management --- the moisture change of coconut palm chaff in observation cuttage vessel, coconut palm chaff color during brown stain yellowly by spraying
Keep the skin wet from the aperture of container edge；
<6>Crop field cuttage --- by step<4>With<5>Pretreatment 20-25 days, treats that cuttings notch expands, callus
Induction is completed, and moves into nutrition cup cuttings carry out conventional culture of rootage at this time.
2. the method that Southern Pine high temperature season cuttings according to claim 1 promotees callus, it is characterised in that：Step<3>In
Cuttage depth be 4-4.5cm.
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| CN201510175430.1A CN104823629B (en) | 2015-04-14 | 2015-04-14 | The method that Southern Pine high temperature season cuttings promotees callus |
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| CN105474959B (en) * | 2015-12-09 | 2018-05-08 | 广西壮族自治区林业科学研究院 | Masson pine tissue-cultured seedling spray single-node cutting seed-raising method |
| CN108605536B (en) * | 2018-04-11 | 2020-06-30 | 中国科学院武汉植物园 | Lateral illumination cuttage method for vanilla rosewood of vanilla |
| CN110235621A (en) * | 2019-07-26 | 2019-09-17 | 云南省农业科学院热区生态农业研究所 | A kind of cottage method of Moringa in Dry-hot Valley Area |
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| CN103583340A (en) * | 2013-11-21 | 2014-02-19 | 信阳农林学院 | Gardenia multi-branch floating bed water planting cuttage method |
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| CN103583340A (en) * | 2013-11-21 | 2014-02-19 | 信阳农林学院 | Gardenia multi-branch floating bed water planting cuttage method |
Non-Patent Citations (2)
| Title |
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| 促进扦插花木快速生根的方法;曹涤环等;《新农村》;20150410;第22-23页 * |
| 温差作用在扦插上的应用;黄建华等;《农业与技术》;20061030;第26卷(第5期);第105-106页 * |
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