CN104813984A - Method for improving fertility rate of andrias davidianus - Google Patents
Method for improving fertility rate of andrias davidianus Download PDFInfo
- Publication number
- CN104813984A CN104813984A CN201510287666.4A CN201510287666A CN104813984A CN 104813984 A CN104813984 A CN 104813984A CN 201510287666 A CN201510287666 A CN 201510287666A CN 104813984 A CN104813984 A CN 104813984A
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- China
- Prior art keywords
- giant salamander
- ovum
- adopting
- platform
- luangou
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000035558 fertility Effects 0.000 title abstract description 7
- 241001253208 Andrias davidianus Species 0.000 title abstract 6
- 230000004720 fertilization Effects 0.000 claims abstract description 20
- 238000011534 incubation Methods 0.000 claims abstract description 7
- 210000000582 semen Anatomy 0.000 claims abstract description 6
- 241000157862 Dicamptodontidae Species 0.000 claims description 54
- 102000002322 Egg Proteins Human genes 0.000 claims description 36
- 108010000912 Egg Proteins Proteins 0.000 claims description 36
- 210000004681 ovum Anatomy 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 210000001015 abdomen Anatomy 0.000 claims description 8
- 230000009027 insemination Effects 0.000 claims description 7
- 230000036541 health Effects 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 239000008187 granular material Substances 0.000 abstract 2
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 230000004913 activation Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000006408 female gonad development Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000023508 male gonad development Effects 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the technical field of andrias davidianus aquaculture, and particularly relates to a method for improving the fertility rate of andrias davidianus, aiming at solving the technical problems that in the prior art, the time for egg collection is long, so that the egg granule activity is poor, and the fertility rate is low. The method for improving the fertility rate of the andrias davidianus comprises the following steps: obtaining seminal fluid with good vitality for later use; obtaining the chalaza of the andrias davidianus on an egg collection table; then, carrying out artificial fertilization and incubation. After the technical scheme is adopted, the fertility rate of the egg granules of the andrias davidianus is increased by 15-30%.
Description
Technical field
The invention belongs to giant salamander culture technical field, be specifically related to a kind of method improving giant salamander fertilization rate.
Background technology
Giant salamander (Andrhts davidionus) is the distinctive rareness species of China; the Evolutionary History of existing 3.5 hundred million years; the amphibian that build is maximum in the world now, have high scientific research, medicinal, health care and edibility, be national second class protection animal.In recent years, because the reason such as the change of circumstances, ecological disruption, water pollution, high strength are fished for and giant salamander fertility is weak, cause the sharply atrophy of giant salamander habitat, wild giant salamander quantity is also fewer and feweri, and some region populations become extinct.In order to protect these rare and endangered species of giant salamander, a large amount of basic scientific researches and captive test, from since 20 century 70s, have been done by China.Although giant salamander artificial insemination in 1978 hatching succeeds; but the large-scale artificial breeding of giant salamander or a difficult problem up to now, its main cause has that giant salamander male and female gonad development is asynchronous, sperm viability is poor and spawning rate, fertility rate and hatchability are not high.
Traditional giant salamander artificial insemination method is after the giant salamander of artificial induced spawning reaches Effect time, allow female giant salamander voluntarily behind output part chalaza, chalaza is drawn artificially in water, help the more mature egg grain of female giant salamander output, but this method is owing to being the ovum grain obtained in water, time is longer, and ovum grain constantly starts water suction under the activation of water, causes fertilization rate to decline.The distinguishing feature of this technology is ovum grain is not obtain in water, has delayed the process of ovum grain water suction, and serve fairly obvious effect to raising giant salamander fertilization rate, fertilization rate improves 15% ~ 30% than traditional method.
Traditional giant salamander artificial insemination method is after the giant salamander of artificial induced spawning reaches Effect time; allow female giant salamander voluntarily behind output part chalaza; in water, chalaza is drawn by external force (artificial mode); help the more mature egg grain of female giant salamander output; when after ovum grain runs up to some again with the giant salamander seminal fluid mixing and stirring obtained in advance after, added water fertilization.
Traditional this ovum method of adopting is owing to being the ovum grain obtained in water, and the time is longer, and ovum grain constantly starts water suction under the activation of water, and when again with giant salamander seminal fluid mix and blend, the ovum grain that part first obtains absorbs water, and causes fertilization rate to decline.
Female giant salamander is placed on common table by some employings, and upwards, downwards (lying on the back), both hands extrude giant salamander belly to belly at back, and such position giant salamander like a hen on a hot girdle, fiercely can struggle, causes chalaza to be ruptured; Simultaneously because squeeze force is comparatively large, the ovum grain distortion of acquisition, causes fertilization rate low.
Summary of the invention
The technical problem to be solved in the present invention is that in prior art, time for oocyte collection is long, causes ovum grain poor activity, fertilization rate low.
The invention provides a kind of method improving giant salamander fertilization rate, comprise the steps:
1) seminal fluid is obtained for subsequent use;
2) the female giant salamander starting a bit of chalaza of output is moved in time adopt ovum platform, keep back upwards, the downward normal posture of belly, make giant salamander cloacal aperture aim at the Ji Luangou adopting ovum platform, and cover towel at giant salamander human head and chest, frequently water to keep the moistening of health; Described ovum platform of adopting is the unsealed cuboid box-like structure in top, and the bottom surface of this structure is set to low early and high after, and when adopting ovum, giant salamander head forward, bottom surface is also provided with the Ji Luangou under shed;
3) collection ovum basin is placed on below the Ji Luangou adopting ovum platform, one people with the hands slowly extrudes to cloacal aperture direction gently from female giant salamander belly, another people gently moves out in the have gentle hands of female giant salamander cloacal aperture a bit of chalaza of output, makes giant salamander egg bands can flow into collection ovum basin smoothly;
4) fish dry method insemination method is adopted to complete fertilization; Fertilized egg is moved into normal incubation in incubation equipment;
More than operate and complete all in the dark.
Beneficial effect of the present invention: technical solution of the present invention can make giant salamander ovum grain fertilization rate raising 15% ~ 30%, and (traditional artificial insemination method is generally 40% ~ 60%; the inventive method fertilization rate is generally 60% ~ 75%); thus improve efficiency and the economic benefit of giant salamander artificial propagation to a certain extent; not enough for extenuating giant salamander seed, crack giant salamander scale artificial culture bottleneck and there is very positive effect.
Accompanying drawing explanation
Fig. 1 floor map of adopting ovum platform used in the present invention;
The cutaway view adopting ovum platform that Fig. 2 the present invention uses; 1-adopts ovum platform, 2-Ji Luangou.
Embodiment
Embodiment
Under the prerequisite signing confidentiality agreement, in August, 2014, Yongfeng, Wulong County, Chongqing City agricultural development Co., Ltd utilizes the inventive method.
1) seminal fluid first obtaining vigor good is for subsequent use;
2) the female giant salamander starting a bit of chalaza of output is moved in time adopt ovum platform, keep back upwards, the downward normal posture of belly, make giant salamander cloacal aperture aim at the Ji Luangou adopting ovum platform, and cover towel at giant salamander human head and chest, frequently water to keep the moistening of health; Described ovum platform of adopting is the unsealed cuboid box-like structure in top, and the bottom surface of this structure is set to low early and high after, and when adopting ovum, giant salamander head forward, bottom surface is also provided with the Ji Luangou under shed;
3) collection ovum basin is placed on below the Ji Luangou adopting ovum platform, one people with the hands slowly extrudes to cloacal aperture direction gently from female giant salamander belly, another people gently moves out in the have gentle hands of female giant salamander cloacal aperture a bit of chalaza of output, makes giant salamander egg bands can flow into collection ovum basin smoothly;
4) fish dry method insemination method is adopted to complete fertilization; Fertilized egg is moved into normal incubation in incubation equipment;
More than operate and complete all in the dark.
Artificial propagation three batches of giant salamanders (amounting to 43 groups of giant salamanders) altogether, average fertilization rate is 67%, and improve 25% and 23% respectively compared with the average fertilization rate 44% of 2013 annual fertilization rate 42% and 2014, effect is fairly obvious.
Claims (1)
1. improve a method for giant salamander fertilization rate, it is characterized in that: comprise the steps:
1) seminal fluid is obtained for subsequent use;
2) the female giant salamander starting a bit of chalaza of output is moved in time adopt ovum platform, keep back upwards, the downward normal posture of belly, make giant salamander cloacal aperture aim at the Ji Luangou adopting ovum platform, and cover towel at giant salamander human head and chest, frequently water to keep the moistening of health; Described ovum platform of adopting is the unsealed cuboid box-like structure in top, and the bottom surface of this structure is set to low early and high after, and when adopting ovum, giant salamander head forward, bottom surface is also provided with the Ji Luangou under shed;
3) collection ovum basin is placed on below the Ji Luangou adopting ovum platform, one people with the hands slowly extrudes to cloacal aperture direction gently from female giant salamander belly, another people gently moves out in the have gentle hands of female giant salamander cloacal aperture a bit of chalaza of output, makes giant salamander egg bands can flow into collection ovum basin smoothly;
4) fish dry method insemination method is adopted to complete fertilization; Fertilized egg is moved into normal incubation in incubation equipment;
More than operate and complete all in the dark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510287666.4A CN104813984B (en) | 2015-05-29 | 2015-05-29 | Method for improving fertility rate of andrias davidianus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510287666.4A CN104813984B (en) | 2015-05-29 | 2015-05-29 | Method for improving fertility rate of andrias davidianus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104813984A true CN104813984A (en) | 2015-08-05 |
| CN104813984B CN104813984B (en) | 2017-05-24 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510287666.4A Expired - Fee Related CN104813984B (en) | 2015-05-29 | 2015-05-29 | Method for improving fertility rate of andrias davidianus |
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| Country | Link |
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| CN (1) | CN104813984B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2501312Y (en) * | 2001-08-10 | 2002-07-24 | 南昌大学 | Culture pool for giant salamander |
| CN1788696A (en) * | 2005-12-20 | 2006-06-21 | 张丙彦 | Emulated ecological environment breeding method and device for giant salamander |
| CN203027904U (en) * | 2012-12-28 | 2013-07-03 | 武汉百瑞生物技术有限公司 | Egg collecting platform |
| CN103283647A (en) * | 2013-05-21 | 2013-09-11 | 天津正跃水产储养有限公司 | Manual intervention method for giant salamander reproduction |
| CN103493783A (en) * | 2013-10-18 | 2014-01-08 | 梅县海霞水产品有限公司 | Method for improving ovum quality of giant salamander parent |
| CN203424158U (en) * | 2013-09-16 | 2014-02-12 | 天全润兆鲟业有限公司 | Sturgeon roe collection device |
| CN203482753U (en) * | 2013-09-25 | 2014-03-19 | 四川省农业科学院水产研究所 | Sturgeon artificial propagation operation platform |
-
2015
- 2015-05-29 CN CN201510287666.4A patent/CN104813984B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2501312Y (en) * | 2001-08-10 | 2002-07-24 | 南昌大学 | Culture pool for giant salamander |
| CN1788696A (en) * | 2005-12-20 | 2006-06-21 | 张丙彦 | Emulated ecological environment breeding method and device for giant salamander |
| CN203027904U (en) * | 2012-12-28 | 2013-07-03 | 武汉百瑞生物技术有限公司 | Egg collecting platform |
| CN103283647A (en) * | 2013-05-21 | 2013-09-11 | 天津正跃水产储养有限公司 | Manual intervention method for giant salamander reproduction |
| CN203424158U (en) * | 2013-09-16 | 2014-02-12 | 天全润兆鲟业有限公司 | Sturgeon roe collection device |
| CN203482753U (en) * | 2013-09-25 | 2014-03-19 | 四川省农业科学院水产研究所 | Sturgeon artificial propagation operation platform |
| CN103493783A (en) * | 2013-10-18 | 2014-01-08 | 梅县海霞水产品有限公司 | Method for improving ovum quality of giant salamander parent |
Non-Patent Citations (1)
| Title |
|---|
| 丁雷: "《淡水鱼养殖技术》", 31 January 2003, 中国农业大学出版社 * |
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| CN104813984B (en) | 2017-05-24 |
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Address after: 402460 Chongqing Changyuan County of Rongchang city Xueyuan Road street, No. 160 Patentee after: SOUTHWEST University Address before: 400715 Chongqing Road, Beibei District No. 2 Patentee before: Southwest University |
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