CN104813984B - Method for improving fertility rate of andrias davidianus - Google Patents
Method for improving fertility rate of andrias davidianus Download PDFInfo
- Publication number
- CN104813984B CN104813984B CN201510287666.4A CN201510287666A CN104813984B CN 104813984 B CN104813984 B CN 104813984B CN 201510287666 A CN201510287666 A CN 201510287666A CN 104813984 B CN104813984 B CN 104813984B
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- China
- Prior art keywords
- giant salamander
- ovum
- platform
- fertilization
- luangou
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 241001253208 Andrias davidianus Species 0.000 title abstract 6
- 230000035558 fertility Effects 0.000 title abstract 5
- 230000004720 fertilization Effects 0.000 claims abstract description 21
- 238000011534 incubation Methods 0.000 claims abstract description 8
- 210000000582 semen Anatomy 0.000 claims abstract description 6
- 241000157862 Dicamptodontidae Species 0.000 claims description 54
- 102000002322 Egg Proteins Human genes 0.000 claims description 36
- 108010000912 Egg Proteins Proteins 0.000 claims description 36
- 210000004681 ovum Anatomy 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 210000001015 abdomen Anatomy 0.000 claims description 8
- 230000009027 insemination Effects 0.000 claims description 7
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 6
- 239000008187 granular material Substances 0.000 abstract 2
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 description 6
- 230000004913 activation Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 206010003694 Atrophy Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000006408 female gonad development Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000023508 male gonad development Effects 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the technical field of andrias davidianus aquaculture, and particularly relates to a method for improving the fertility rate of andrias davidianus, aiming at solving the technical problems that in the prior art, the time for egg collection is long, so that the egg granule activity is poor, and the fertility rate is low. The method for improving the fertility rate of the andrias davidianus comprises the following steps: obtaining seminal fluid with good vitality for later use; obtaining the chalaza of the andrias davidianus on an egg collection table; then, carrying out artificial fertilization and incubation. After the technical scheme is adopted, the fertility rate of the egg granules of the andrias davidianus is increased by 15-30%.
Description
Technical field
The invention belongs to giant salamander culture technical field, and in particular to a kind of method of raising giant salamander rate of fertilization.
Background technology
Giant salamander (Andrhts davidionus) is the distinctive rareness species of China, existing 3.5 hundred million years Evolutionary History, is existing
The maximum amphibian of build, is national second class protection with high scientific research, medicinal, health care and edibility on this life circle
Animal.In recent years, because the change of circumstances, ecological disruption, water pollution, high intensity are fished for and the reason such as giant salamander reproductive capacity is weak,
Cause the drastically atrophy of giant salamander habitat, wild giant salamander quantity is also fewer and feweri, and some region populations have become extinct.In order to protect
Substantial amounts of basic scientific research and artificial domestication have done since 20 century 70s in giant salamander this rare and endangered species, China
Reproduction test.Although the hatching of giant salamander artificial insemination in 1978 has succeeded, the large-scale artificial breeding of giant salamander up to now
Or a problem, its main cause have asynchronous giant salamander male and female gonad development, poor sperm motility and spawning rate, rate of fertilization and
Incubation rate is not high.
Traditional giant salamander artificial insemination method is after the giant salamander of artificial induced spawning reaches Effect time, to allow female giant salamander voluntarily
Behind the chalaza of output part, chalaza is artificially drawn in water, help the more mature egg grains of female giant salamander output, but this method
Due to being the ovum grain obtained in water, the time is more long, and ovum grain constantly starts water suction under the activation of water, causes rate of fertilization to decline.
The distinguishing feature of this technology is that ovum grain is obtained in water, has delayed the process of ovum grain water suction, is risen to improving giant salamander rate of fertilization
Fairly obvious effect is arrived, rate of fertilization improves 15%~30% than traditional method.
Traditional giant salamander artificial insemination method is after the giant salamander of artificial induced spawning reaches Effect time, to allow female giant salamander voluntarily
Behind the chalaza of output part, chalaza is drawn in water by external force (artificial mode), help the female giant salamander more mature eggs of output
Grain, after ovum grain runs up to certain amount again with the prior giant salamander seminal fluid mixing and stirring for obtaining after, add water completion fertilization.
Traditional this ovum method of adopting is due to being the ovum grain obtained in water, and the time is more long, ovum grain under the activation of water not
Water suction of beginning is disconnected, when being mixed with giant salamander seminal fluid again, the ovum grain that part first obtains has absorbed water, and causes rate of fertilization to decline.
Be placed on female giant salamander on common table by some uses, and upwards, downwards (lying on the back), both hands extrude giant salamander abdomen to belly at back
Portion, such position giant salamander like a hen on a hot girdle, fierce can struggle, and cause chalaza to be broken；Simultaneously because squeeze force is larger, acquisition
Ovum grain deformation, causes rate of fertilization low.
The content of the invention
The technical problem to be solved in the present invention is that time for oocyte collection is long in the prior art, causes ovum grain poor activity, rate of fertilization low.
The invention provides a kind of method for improving giant salamander rate of fertilization, comprise the following steps：
1) seminal fluid is obtained standby；
2) ovum platform is adopted in the female giant salamander immigration in time that will have begun to a bit of chalaza of output, and holding back is upward, belly
Downward normal posture, makes giant salamander cloacal aperture be directed at the Ji Luangou for adopting ovum platform, and covers towel in giant salamander human head and chest,
Water to keep the moistening of body frequently；Described adopts ovum platform for the unsealed cuboid box-like structure in top, the bottom of the structure
Face be set to it is low early and high after, when adopting ovum giant salamander head be additionally provided with downwardly open Ji Luangou forward, on bottom surface；
3) collection ovum basin is placed on below the Ji Luangou for adopting ovum platform, a people is with the hands from female giant salamander belly gently to cloacal aperture
Direction slowly extrudes, and another people gently moves out a bit of chalaza of output in female giant salamander cloacal aperture hand, makes giant salamander egg bands
Collection ovum basin can smoothly be flowed into；
4) fertilization is completed using fish dry method insemination method；By normal incubation in embryonated egg immigration incubation equipment；
Operation above is completed in the dark.
Beneficial effects of the present invention：Technical solution of the present invention can make giant salamander ovum grain rate of fertilization improve 15%~30% (biography
The artificial insemination method of system is usually 40%~60%, and the inventive method rate of fertilization is usually 60%~75%), so as to certain
The efficiency and economic benefit of giant salamander artificial propagation are improved in degree, it is not enough for extenuating giant salamander seed, crack giant salamander scale people
Work cultivation bottleneck has very positive effect.
Brief description of the drawings
Fig. 1 floor map for adopting ovum platform used in the present invention；
What Fig. 2 present invention was used adopts the sectional view of ovum platform；1- adopts ovum platform, 2- Ji Luangou.
Specific embodiment
Embodiment
On the premise of confidentiality agreement is signed, in August, 2014, Chongqing City Wulong County Yongfeng agricultural development Co., Ltd utilizes
The inventive method.
1) the good seminal fluid of vigor is first obtained standby；
2) ovum platform is adopted in the female giant salamander immigration in time that will have begun to a bit of chalaza of output, and holding back is upward, belly
Downward normal posture, makes giant salamander cloacal aperture be directed at the Ji Luangou for adopting ovum platform, and covers towel in giant salamander human head and chest,
Water to keep the moistening of body frequently；Described adopts ovum platform for the unsealed cuboid box-like structure in top, the bottom of the structure
Face be set to it is low early and high after, when adopting ovum giant salamander head be additionally provided with downwardly open Ji Luangou forward, on bottom surface；
3) collection ovum basin is placed on below the Ji Luangou for adopting ovum platform, a people is with the hands from female giant salamander belly gently to cloacal aperture
Direction slowly extrudes, and another people gently moves out a bit of chalaza of output in female giant salamander cloacal aperture hand, makes giant salamander egg bands
Collection ovum basin can smoothly be flowed into；
4) fertilization is completed using fish dry method insemination method；By normal incubation in embryonated egg immigration incubation equipment；
Operation above is completed in the dark.
Three batches of giant salamanders of common artificial propagation (amount to 43 groups of giant salamanders), and average rate of fertilization is 67%, compared with 2013 annual rates of fertilization
42% and the average rate of fertilization 44% of 2014 be respectively increased 25% and 23%, effect is fairly obvious.
Claims (1)
1. it is a kind of improve giant salamander rate of fertilization method, it is characterised in that：Comprise the following steps：
1) seminal fluid is obtained standby；
2) ovum platform is adopted in the female giant salamander immigration in time that will have begun to a bit of chalaza of output, and holding back is upward, belly is downward
Normal posture, be directed at giant salamander cloacal aperture and adopt the Ji Luangou of ovum platform, and towel is covered in giant salamander human head and chest, frequently
Water to keep the moistening of body；Described adopts ovum platform for the unsealed cuboid box-like structure in top, and the bottom surface of the structure sets
Be set to it is low early and high after, when adopting ovum giant salamander head be additionally provided with downwardly open Ji Luangou forward, on bottom surface；
3) collection ovum basin is placed on below the Ji Luangou for adopting ovum platform, a people is with the hands from female giant salamander belly gently to cloacal aperture direction
Slow extruding, another people gently moves out a bit of chalaza of output in female giant salamander cloacal aperture hand, enables giant salamander egg bands suitable
The inflow collection ovum basin of profit；
4) fertilization is completed using fish dry method insemination method；By normal incubation in embryonated egg immigration incubation equipment；
Operation above is completed in the dark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510287666.4A CN104813984B (en) | 2015-05-29 | 2015-05-29 | Method for improving fertility rate of andrias davidianus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510287666.4A CN104813984B (en) | 2015-05-29 | 2015-05-29 | Method for improving fertility rate of andrias davidianus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104813984A CN104813984A (en) | 2015-08-05 |
| CN104813984B true CN104813984B (en) | 2017-05-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510287666.4A Expired - Fee Related CN104813984B (en) | 2015-05-29 | 2015-05-29 | Method for improving fertility rate of andrias davidianus |
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| CN (1) | CN104813984B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2501312Y (en) * | 2001-08-10 | 2002-07-24 | 南昌大学 | Culture pool for giant salamander |
| CN1788696A (en) * | 2005-12-20 | 2006-06-21 | 张丙彦 | Emulated ecological environment breeding method and device for giant salamander |
| CN203027904U (en) * | 2012-12-28 | 2013-07-03 | 武汉百瑞生物技术有限公司 | Egg collecting platform |
| CN103283647A (en) * | 2013-05-21 | 2013-09-11 | 天津正跃水产储养有限公司 | Manual intervention method for giant salamander reproduction |
| CN103493783A (en) * | 2013-10-18 | 2014-01-08 | 梅县海霞水产品有限公司 | Method for improving ovum quality of giant salamander parent |
| CN203424158U (en) * | 2013-09-16 | 2014-02-12 | 天全润兆鲟业有限公司 | Sturgeon roe collection device |
| CN203482753U (en) * | 2013-09-25 | 2014-03-19 | 四川省农业科学院水产研究所 | Sturgeon artificial propagation operation platform |
-
2015
- 2015-05-29 CN CN201510287666.4A patent/CN104813984B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2501312Y (en) * | 2001-08-10 | 2002-07-24 | 南昌大学 | Culture pool for giant salamander |
| CN1788696A (en) * | 2005-12-20 | 2006-06-21 | 张丙彦 | Emulated ecological environment breeding method and device for giant salamander |
| CN203027904U (en) * | 2012-12-28 | 2013-07-03 | 武汉百瑞生物技术有限公司 | Egg collecting platform |
| CN103283647A (en) * | 2013-05-21 | 2013-09-11 | 天津正跃水产储养有限公司 | Manual intervention method for giant salamander reproduction |
| CN203424158U (en) * | 2013-09-16 | 2014-02-12 | 天全润兆鲟业有限公司 | Sturgeon roe collection device |
| CN203482753U (en) * | 2013-09-25 | 2014-03-19 | 四川省农业科学院水产研究所 | Sturgeon artificial propagation operation platform |
| CN103493783A (en) * | 2013-10-18 | 2014-01-08 | 梅县海霞水产品有限公司 | Method for improving ovum quality of giant salamander parent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104813984A (en) | 2015-08-05 |
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Address after: 402460 Chongqing Changyuan County of Rongchang city Xueyuan Road street, No. 160 Patentee after: SOUTHWEST University Address before: 400715 Chongqing Road, Beibei District No. 2 Patentee before: Southwest University |
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