CN103283647A - Manual intervention method for giant salamander reproduction - Google Patents
Manual intervention method for giant salamander reproduction Download PDFInfo
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- CN103283647A CN103283647A CN2013101896661A CN201310189666A CN103283647A CN 103283647 A CN103283647 A CN 103283647A CN 2013101896661 A CN2013101896661 A CN 2013101896661A CN 201310189666 A CN201310189666 A CN 201310189666A CN 103283647 A CN103283647 A CN 103283647A
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- giant salamander
- ovum
- hatching
- belly
- manual intervention
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a manual intervention method for giant salamander reproduction. The manual intervention method sequentially and continuously includes the steps of pulling eggs, semen collection, insemination, incubation and the like. The steps are performed at constant temperature of 17-23 DEG C. The incubation can be performed in micro-flowing water or in still water. The manual intervention method is used for reproduction of cultured giant salamanders, and the success rate of giant salamander artificial reproduction in northern area is increased.
Description
Technical field
The present invention relates to the breed of giant salamander.
Background technology
Giant salamander also claims the giant salamander, belongs to national second class protection animal, mainly originate in the mountain stream river in the Changjiang river, the Yellow River and Zhujiang River middle and upper reaches tributary, and be many with provinces such as Sichuan, Hubei, Hunan, Guizhou, Shaanxi especially.Giant salamander happiness the moon keeps in dark place, and growth rate is slower, and taking 5～6 years under the carded sliver spare out of office can sexual maturity, so its breeding difficulty comparatively, and especially at northern area, giant salamander artificial propagation success rate is lower.
Summary of the invention
The purpose of this invention is to provide a kind of manual intervention method of giant salamander breeding, can improve northern area giant salamander artificial propagation success rate.
In order to achieve the above object, the technical solution used in the present invention is:
A kind of manual intervention method of giant salamander breeding is characterized in that: may further comprise the steps,
A. draw ovum, to propagating the female giant salamander in 7-8 years artificially, after observation has the chalaza output, giant salamander has been caught gently, the wet cloth of a human is muffled the head of giant salamander, and the giant salamander belly is lain in tables or the bigger basin up, holds the head of giant salamander gently; Another people holds the afterbody of giant salamander on the other hand, on the other hand the belly of patting giant salamander that does not stop; The 3rd people then can be pulled outwardly ovum gently, takes advantage of a situation and put in anhydrous clean the porcelain basin or beaker in the chalaza of pulling out, and after adopting ovum and finishing, ovum is built with another clean basin or husky cloth, waits for insemination;
B. semen collection, same quadrat method, will to propagate artificially 7-8 years male giant salamander belly up, pat the giant salamander belly it held; The light belly of pressing drains the urine in the bladder earlier, then with stand-by in the seminal fluid income test tube of extruding;
C. insemination, the sperm that the b step is adopted are added drop-wise on the ovum that a step adopts, and shake the container of containing ovum gently, and smart ovum was fully mixed 5-8 minutes, add a small amount of distilled water, stop 18-22 minutes, wash ovum twice with clear water;
D. hatching is put into incubator box with the ovum after the cleaning of c step and is hatched, and the hatching water temperature is controlled at 18-22 ℃, more than the dissolved oxygen in water 5mg/L, and 800-1000/m of hatching density
3, the depth of water 8-12cm.
Above-described a, b, c step are carried out at constant temperature, and its temperature is 17-23 ℃.
Hatching can be adopted the hatching of miniflow water or hydrostatic hatching in the above d step.
The invention has the beneficial effects as follows, adopt the method for manual intervention, the giant salamander of propagating artificially is bred, improve northern area giant salamander artificial propagation success rate.
Embodiment
Generally take 5～6 years under the giant salamander carded sliver spare out of office and reach sexual maturity, will a little later when carrying out artificial insemination, need reach 7-8 years and just can carry out and propagate artificially also.Under southern wild environment, generally oestrus in 8-October, and must finish fertilization in September in the past in the north, could guarantee the success rate of giant salamander artificial insemination hatching.
The present invention adopts and draws ovum, semen collection, insemination, four steps of hatching to carry out manual intervention, enter September, at first, to propagating the female giant salamander in 8 years artificially, after observation has the chalaza output, giant salamander have been caught gently, the wet cloth of a human is muffled the head of giant salamander, the giant salamander belly is lain in tables or the bigger basin up, hold the head of giant salamander gently; Another people holds the afterbody of giant salamander on the other hand, on the other hand the belly of patting giant salamander that does not stop; The 3rd people then can be pulled outwardly ovum gently, takes advantage of a situation and put in anhydrous clean the porcelain basin or beaker in the chalaza of pulling out, and after adopting ovum and finishing, ovum is built with another clean basin or husky cloth, waits for insemination;
Same quadrat method then, will to propagate artificially 8 years male giant salamander belly up, pat the giant salamander belly it held; The light belly of pressing drains the urine in the bladder earlier, then with stand-by in the seminal fluid income test tube of extruding;
In the 3rd step, the sperm that above step is adopted is added drop-wise on the ovum of adopting, and shakes the container of containing ovum gently, and smart ovum was fully mixed 6 minutes, adds a small amount of distilled water, stops 20 minutes, washes ovum twice with clear water;
At last, the ovum after the above step cleaning is put into incubator box carry out the hydrostatic hatching, the hatching water temperature is controlled at 22 ℃, more than the dissolved oxygen in water 5mg/L, and 900/m of hatching density
3, depth of water 10cm.And will make regular check on the dead ovum of timely cleaning, in case infect good fertilized egg or embryo.Certainly, hatching also can be hatched under the miniflow water environment, and effect is desirable equally.
Should be noted that embodiment of the present invention only is its optimal way, to belonging to general idea of the present invention, and only is apparent change, all should belong within protection scope of the present invention.
Claims (3)
1. the manual intervention method of giant salamander breeding is characterized in that: may further comprise the steps,
A. draw ovum, to propagating the female giant salamander in 7-8 years artificially, after observation has the chalaza output, giant salamander has been caught gently, the wet cloth of a human is muffled the head of giant salamander, and the giant salamander belly is lain in tables or the bigger basin up, holds the head of giant salamander gently; Another people holds the afterbody of giant salamander on the other hand, on the other hand the belly of patting giant salamander that does not stop; The 3rd people then can be pulled outwardly ovum gently, takes advantage of a situation and put in anhydrous clean the porcelain basin or beaker in the chalaza of pulling out, and after adopting ovum and finishing, ovum is built with another clean basin or husky cloth, waits for insemination;
B. semen collection, same quadrat method, will to propagate artificially 7-8 years male giant salamander belly up, pat the giant salamander belly it held; The light belly of pressing drains the urine in the bladder earlier, then with stand-by in the seminal fluid income test tube of extruding;
C. insemination, the sperm that the b step is adopted are added drop-wise on the ovum that a step adopts, and shake the container of containing ovum gently, and smart ovum was fully mixed 5-8 minutes, add a small amount of distilled water, stop 18-22 minutes, wash ovum twice with clear water;
D. hatching is put into incubator box with the ovum after the cleaning of c step and is hatched, and the hatching water temperature is controlled at 18-22 ℃, more than the dissolved oxygen in water 5mg/L, and 800-1000/m of hatching density
3, the depth of water 8-12cm.
2. the manual intervention method of giant salamander according to claim 1 breeding, it is characterized in that: described a, b, c step are carried out at constant temperature, and its temperature is 17-23 ℃.
3. the manual intervention method of giant salamander breeding according to claim 1 is characterized in that: hatch in the described d step and can adopt the hatching of miniflow water or hydrostatic hatching.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101896661A CN103283647A (en) | 2013-05-21 | 2013-05-21 | Manual intervention method for giant salamander reproduction |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101896661A CN103283647A (en) | 2013-05-21 | 2013-05-21 | Manual intervention method for giant salamander reproduction |
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| CN103283647A true CN103283647A (en) | 2013-09-11 |
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| CN2013101896661A Pending CN103283647A (en) | 2013-05-21 | 2013-05-21 | Manual intervention method for giant salamander reproduction |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104813984A (en) * | 2015-05-29 | 2015-08-05 | 西南大学 | Method for improving fertility rate of andrias davidianus |
-
2013
- 2013-05-21 CN CN2013101896661A patent/CN103283647A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104813984A (en) * | 2015-05-29 | 2015-08-05 | 西南大学 | Method for improving fertility rate of andrias davidianus |
| CN104813984B (en) * | 2015-05-29 | 2017-05-24 | 西南大学 | Method for improving fertility rate of andrias davidianus |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130911 |