CN104782455B - Matrix and its application for tobacco axillary bud cutting propagation - Google Patents
Matrix and its application for tobacco axillary bud cutting propagation Download PDFInfo
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- CN104782455B CN104782455B CN201410023203.2A CN201410023203A CN104782455B CN 104782455 B CN104782455 B CN 104782455B CN 201410023203 A CN201410023203 A CN 201410023203A CN 104782455 B CN104782455 B CN 104782455B
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- 239000011159 matrix material Substances 0.000 title claims abstract description 76
- 241000208125 Nicotiana Species 0.000 title claims abstract description 55
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 55
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000004021 humic acid Substances 0.000 claims abstract description 21
- 239000010451 perlite Substances 0.000 claims abstract description 13
- 235000019362 perlite Nutrition 0.000 claims abstract description 13
- 239000010903 husk Substances 0.000 claims abstract description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 11
- 235000009566 rice Nutrition 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 240000007594 Oryza sativa Species 0.000 claims abstract 4
- 238000000034 method Methods 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 7
- 235000019504 cigarettes Nutrition 0.000 abstract description 10
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 3
- 230000021749 root development Effects 0.000 abstract description 3
- 230000028446 budding cell bud growth Effects 0.000 abstract description 2
- 230000002209 hydrophobic effect Effects 0.000 abstract description 2
- 238000004904 shortening Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 230000004083 survival effect Effects 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 241000209094 Oryza Species 0.000 description 7
- 239000002689 soil Substances 0.000 description 7
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 description 6
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 description 6
- 239000002509 fulvic acid Substances 0.000 description 6
- 229940095100 fulvic acid Drugs 0.000 description 6
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
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- 239000002253 acid Substances 0.000 description 3
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- 150000003839 salts Chemical class 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 241000921313 Phyllopodium Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
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- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of matrix for tobacco axillary bud cutting propagation and its application, the matrix is by part A haydite, part B mixed-matrix composition；Mixed-matrix is made up of the raw material of following percentage by volumes：Turf 50 60%, perlite 20 30%, carbonized rice husks 10 20%, humic acid 5 10%.Matrix of the present invention is directed to tobacco axillary bud cuttage and seedling culture, according to demand of the root development of tobacco axillary bud cuttage to matrix environment, tested through inventor with studying, matrix is divided into haydite part and by turf, perlite, the mixed-matrix part of carbonized rice husks and humic acid composition, when using, haydite is placed in above mixed-matrix, support is risen to tobacco axillary bud, the effect of fixed and hydrophobic, air-permeability, mixed-matrix provides nutriment for tobacco axillary bud growth, root system is promoted to sprout and grow, the branch of this kind of matrix, which sets up separately to put, makes tobacco axillary bud cuttage obtain higher success rate, simultaneously than the breeding time with the notable shortening cigarette strain of seed seedling-raising.
Description
Technical field
The present invention relates to the matrix field of cutting propagation, more particularly to a kind of matrix for tobacco axillary bud cutting propagation
And its application.
Background technology
During tobacco breeding, usually due to Climatic Effects, make in some weak tendency germ plasm resources or growth course
Producing the Special germplasm resource of variation can not yield positive results, and cause germ plasm resource to reserve seed for planting preservations, or in order to expand as early as possible
Remain the germ plasm resource colony of indivedual plant.
Tobacco axillary bud, refers to the normal bud born from axil.Occur from shoot tip meristem after new phyllopodium, soon its spire
The auxiliary primodia of former base breaks up at once.Form an axillary bud between usual each axil, all axillary buds can be sprouted and be formed point
Branch.Before flowering, only one of which axillary bud in each axil, when will bloom, axillary bud is gradually sprouted, while axillary bud is close to blade
Two or three accessory buds are simultaneously produced, when the continued growth of tobacco top, because the hormone on top is to the inhibitory action of axillary bud, below
Bud generally can not be sprouted.But after cigarette strain is pinched, apical dominance is relieved, the axillary bud of cigarette strain is sprouted in succession.
Cutting propagation, is a kind of conventional vegetative propagation methods for cultivating plant.Can be with the stem of clip plant, leaf, root, bud
Deng in insertion soil, Sha Zhong, or being immersed in water, can be planted after taking root, as independent new plant.The matrix that cuttage is used
The loose ventilation of type requirements, without the organic matter and salt not become thoroughly decomposed.Conventional matrix has river sand, vermiculite, turfy soil, sawdust
Deng generally just with a class or the mixing of two classes.
It is more harsh to environmental condition demand during due to tobacco axillary bud cuttage, and at present because cuttage is only with a class matrix
Or two class mixing, its internal physical character is single, often leads to that permeability is poor, and water balance is low.Time one is grown, and easily causes life
Length is bad, and cuttage end is withered, and causes cuttage to fail.Northwest University Chen Gang etc., was once carried out using sand culture cuttage to k346 variants
Rescue, but the survival rate of cuttage is not described.
The content of the invention
Based on this, during being preserved in tobacco germplasm, to only having the germ plasm resource rapid expansion of a small amount of plant
Colony carries out rescue protection to breeding the big Special germplasm resources of difficulty, and the present invention provides a kind of for tobacco axillary bud skewer
Matrix and its application of breeding are inserted, realizes that tobacco axillary bud breeds plant and obtains tobacco seed using this kind of matrix, so as to reach pair
Only a small amount of plant germ plasm resource or breed the big Special germplasm resources of difficulty preservation and expand it is numerous.
The concrete technical scheme for solving above-mentioned technical problem is as follows：
A kind of matrix for tobacco axillary bud cutting propagation, the matrix is made up of A, B two parts, wherein, part A is pottery
Grain, part B is mixed-matrix；
The mixed-matrix is made up of the raw material of following percentage by volumes：Turf 50-60%, perlite 20-30%, be carbonized paddy
Shell 10-20%, humic acid 5-10%.
In wherein some embodiments, the particle diameter of the haydite is 6-8mm.
In wherein some embodiments, the mixed-matrix is made up of the raw material of following percentage by volumes：Turf 54-56%,
Perlite 23-27%, carbonized rice husks 10-15%, humic acid 6-10%.
A kind of method of tobacco axillary bud cutting propagation, using above-mentioned matrix, part A haydite is laid in part B when using
Above mixed-matrix, haydite layer and mixed-matrix layer are formed, the thickness of haydite layer is 4-5cm, and the thickness of mixed-matrix layer is
11-13cm。
Humic acid is a kind of natural organic substance, based on humic acid and fulvic acid.Humic acid is that a class can be dissolved in alkali
Solution and the humic matter precipitated by acid solution, its molecular weight are bigger than fulvic acid, and the percentage of each element contains in molecular composition
Measuring is respectively：C：50%~60%, H：2.8%~6.6%, O：31%~40%, N：2.6%~6.0%.Acidity of the humic acid than fulvic acid
Small, in subacidity, absorptive capacity is higher, and its monovalence salt is dissolved in water, and divalence and trivalent species are water insoluble, and this is supported to soil
The holding and the formation of soil texture divided all has meaning.Fulvic acid is that a class had not only been dissolved in aqueous slkali but also had been dissolved in the humic of acid solution
Metallic substance, its molecular weight is smaller than humic acid, and the percentage composition of each element is respectively in molecular composition：C：40%~52%, H:4%~
6%, O40%~48%, N2%~6%.Fulvic acid is in highly acid, and mobility is big, and absorbability is lower than humic acid, therefore fulvic acid is to promoting
Enter the decomposition of mineral and the release of nutrient plays an important roll.Humic acid contains a variety of functional groups, the humic acid after being activated into
For efficient bio-active material, there is stimulation to crop growth and body physiological metabolism, this characteristic is general fertilizers
Not available, the humic acid efficient bio-active material after activation, using seed soaking, soaks root by finite concentration, root dipping, sprays, pours
Fill, do the modes such as base fertilizer, there is obvious effect of stimulation to various crops, general performance is promoting crop root development.
A kind of matrix for tobacco axillary bud cutting propagation of the present invention has the following advantages that and beneficial effect：
1st, matrix of the present invention is to be directed to tobacco axillary bud cuttage and seedling culture, according to the root development of tobacco axillary bud cuttage to base
The demand of matter environment, through the substantial amounts of experiment of inventor and research, is divided into haydite part and by turf, perlite, carbonization by matrix
The mixed-matrix part of husk and humic acid composition, in use, haydite part is laid in mixed-matrix part top, haydite water holding
Property it is low, it is to avoid tobacco axillary bud cuttage position is rotted, and simultaneously plays support, fixation and hydrophobic, air-permeability to tobacco axillary bud；
Mixed-matrix each component is worked in coordination and provides nutriment for tobacco axillary bud growth, promotes root system to sprout and grow, this kind of matrix
Branch set and tobacco axillary bud cuttage is obtained higher tobacco axillary bud cuttage success rate, while compared with seed seedling-raising, cigarette
Careless axil bud cutting significantly shorten the breeding time of cigarette strain, you can effectively improve tobacco breeding and seed production efficiency.
2nd, the matrix of the present invention for tobacco axillary bud cutting propagation, it causes weak tendency tobacco germplasm colony to carry out
Expand numerous, the strain of indivedual tobacco mutants is rescued.
Brief description of the drawings
Fig. 1 be embodiment 1 in be used for tobacco axillary bud cutting propagation matrix application schematic diagram.
Description of reference numerals：
1st, haydite layer；2nd, mixed-matrix layer.
Embodiment
Humic acid of the present invention（Butt）≥55%；pH：9-11；Moisture content≤15%；Potassium content >=8%；Water-insoluble≤
5%。
Below with reference to specific embodiment, the invention will be further described.
Embodiment 1
A kind of matrix for tobacco axillary bud cutting propagation, the matrix is made up of A, B two parts, wherein, part A is pottery
Grain, part B is mixed-matrix；The particle diameter of the haydite is 6-8mm；The mixed-matrix by following percentage by volumes raw material group
Into：Turf 55%, perlite 25%, carbonized rice husks 10%, humic acid 10%；Wherein, the physical behavior and nutrient of mixed-matrix
Content is shown in Tables 1 and 2.
The physical behavior of the mixed-matrix of table 1
| Matrix title | Capacity g/cm | Total porosity % | Porosity of ventilating % | Water holding space % | Gas-water ratio |
| Mixed-matrix | 0.15 | 82.0 | 30.0 | 52.2 | 1:8.33 |
Nutritive element content in the mixed-matrix of table 2
In actual use, the cottage method of tobacco axillary bud comprises the following steps：
（1）Uniform clean free from admixture, particle, medium sized haydite, turf, perlite and carbonized rice husks are selected, and are pressed
Above-mentioned percentage by volume weighs turf, perlite, carbonized rice husks and humic acid and fully mixed；
（2）During sabot, haydite part is laid in above mixed-matrix, top haydite layer and bottom mixed-matrix is formed
Layer, schematic diagram refers to Fig. 1, and the thickness of the haydite layer is 4-5cm, and the thickness of mixed-matrix layer is 11-13cm, and is filled once
It is permeable, note often keeping plugging soil and the humidity of air later, carry out soil moisture conservation work；
（3）The tobacco axillary bud with 4-6 piece leaves is taken from maternal plant, is cleaned up with clear water, axillary bud is inserted into mixed-matrix
In（It is embedded to 2-3cm）；
（4）Before axillary bud is not taken root, it should prevent rainwater from splashing soil on tender leaf.After axillary bud is taken root, it should be noted that carry out
To cooling of shading before at 4 points in afternoon after shade, 10 points of every morning, while spraying water daily, to keep humidity.It is close with plastic greenhouse
When sealing cuttage, water drenching number of times can be reduced, 1~2 time weekly.
（5）After axil bud cutting is survived, appropriate water and fertilizer management should be carried out, meanwhile, hardening is carried out, it is gradually adapted to extraneous ring
Border is transplanted to crop field again.In greenhouse or hotbed during cuttage, after axillary bud takes root lamina, gradually open a window ventilating air, with suitable
External environment is answered, crop field is then transplanted to again.
Embodiment 2
A kind of matrix for tobacco axillary bud cutting propagation, the matrix is made up of A, B two parts, wherein, the part A
For haydite, part B is mixed-matrix；The particle diameter of haydite is 6-8mm；The mixed-matrix by following percentage by volumes raw material group
Into：Turf 50%, perlite 30%, carbonized rice husks 15%, humic acid 5%.
In practical application, the cottage method of tobacco axillary bud, referring to embodiment 1.
Embodiment 3
A kind of matrix for tobacco axillary bud cutting propagation, the matrix is made up of A, B two parts, wherein, part A is pottery
Grain, part B is mixed-matrix；The particle diameter of haydite is 6-8mm；The mixed-matrix is made up of the raw material of following percentage by volumes：
Turf 60%, perlite 20%, carbonized rice husks 10%, humic acid 10%.
In practical application, the cottage method of tobacco axillary bud, referring to embodiment 1.
Evaluate cuttage success rate and the influence of cigarette strain breeding time of the embodiment 1-3 matrix to tobacco axillary bud
First, experiment purpose
Pass through the cuttage success rate of tobacco axillary bud and cigarette strain breeding time described in comparative evaluation's embodiment 1-3.
2nd, experimental method
This experiment is divided into control group and experimental group, wherein, control group is comparative example 1, comparative example 2 and comparative example 3, experimental group
For embodiment 1, embodiment 2 and embodiment 3.
Comparative example 1：30 tobacco seed sproutings are taken, germination percentage is 95%, by the seeds cultivation of sprouting to squaring period, record
Required number of days and survival rate.
Comparative example 2：A kind of conventional substrate for tobacco axillary bud cutting propagation, the matrix is by following percentage by volumes
Raw material is constituted：Turf 60%, perlite 30%, carbonized rice husks 10%.In practical application, the cottage method of tobacco axillary bud, with reference to implementation
Example 1；And record number of days and survival rate needed for tobacco axillary bud grows to squaring period.
Comparative example 3：A kind of river sand for tobacco axillary bud cutting propagation, the river sand particle diameter is about 2-3mm.Practical application
In, the cottage method of tobacco axillary bud, with reference to embodiment 1；And record number of days and survival rate needed for tobacco axillary bud grows to squaring period.
3rd, experimental result
As a result referring to table 3.As can be seen from Table 3：Compared with comparative example 1, the survival rate of embodiment 1-3 tobacco axillary buds is lower slightly,
But more than 80% can be reached, the number of days needed for growing to cigarette strain squaring period also significantly shortens；Compared with comparative example 2-3, implement
The survival rate of example 1-3 tobacco axillary buds is higher, and the number of days needed for growing to cigarette strain squaring period significantly shortens.
The control group of table 3 and experimental group grow to the survival rate and required number of days in cigarette strain squaring period
| Example | Survival rate | Number of days |
| Comparative example 1 | 95% | 147 |
| Comparative example 2 | 55% | 75 |
| Comparative example 3 | 50% | 85 |
| Embodiment 1 | 92% | 65 |
| Embodiment 2 | 83% | 68 |
| Embodiment 3 | 85% | 68 |
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (4)
1. a kind of matrix for tobacco axillary bud cutting propagation, it is characterised in that the matrix is made up of A, B two parts, wherein,
Part A is haydite, and part B is mixed-matrix；
The haydite is laid in above the mixed-matrix, and the top for making the matrix for tobacco axillary bud cutting propagation is
Haydite layer, bottom are mixed-matrix layer；
The mixed-matrix is made up of the raw material of following percentage by volumes：Turf 50-60%, perlite 20-30%, carbonized rice husks
10-20%, humic acid 5-10%.
2. the matrix according to claim 1 for tobacco axillary bud cutting propagation, it is characterised in that the mixed-matrix by
The raw material composition of following percentage by volumes：Turf 54-56%, perlite 23-27%, carbonized rice husks 10-15%, humic acid 6-
10%.
3. the matrix according to claim 1 for tobacco axillary bud cutting propagation, it is characterised in that the particle diameter of the haydite
For 6-8mm.
4. a kind of method of tobacco axillary bud cutting propagation, it is characterised in that usage right requires the matrix described in any one of 1-3,
Part A haydite is laid in above part B mixed-matrix when using, and forms haydite layer and mixed-matrix layer, the haydite layer
Thickness is 4-5cm, and the thickness of mixed-matrix layer is 11-13cm.
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| CN105309290B (en) * | 2015-10-13 | 2017-12-08 | 云南省烟草农业科学研究院 | A kind of cultural method of flue-cured tobacco regeneration plant and application |
| CN109429987A (en) * | 2018-11-29 | 2019-03-08 | 安徽泓森高科林业股份有限公司 | A kind of locust tree root cutting growth matrix |
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| CN102696302B (en) * | 2012-07-03 | 2014-02-19 | 广西壮族自治区林业科学研究院 | Teak low-chipper sprout-promotion rapid-propagation seed-growing method |
| CN102845288A (en) * | 2012-09-12 | 2013-01-02 | 云南省烟草公司玉溪市公司 | Biochar substrate for raising tobacco seedlings and method for raising tobacco seedlings by biochar substrate |
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| CN103229706B (en) * | 2013-05-20 | 2015-07-01 | 湖南省烟草公司永州市公司 | Organic matrix for culturing tobacco seedlings |
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