CN104737990A - Method for artificially breeding great amounts of pea aphids - Google Patents
Method for artificially breeding great amounts of pea aphids Download PDFInfo
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Abstract
The invention belongs to the field of green pest insect prevention and control, relates to a method for artificially breading great amounts of pea aphids. The method includes the following steps of a, processing seeds, b, filling substrate and sowing, c, inputting a floating disc into water, d, seedling vaccination, e, aphid stock, f, seedling shearing and aphid feeding, g, later-period processing and h circulating. The method for artificially breading the pea aphids relates to the artificial expanding breeding method of living body food insects, and the method is suitable for artificial feeding of natural enemy insects.
Description
Technical field
The present invention relates to artificial a large amount of method expanding numerous acyrthosiphum pisim.
Background technology
The biological control of Phytophthira insect mainly relies on the field adaptability of natural enemy insect (as harmonia axyridia, coccinella septempunctata, Propylaea japonica, how different ladybug, thin chest syrphus fly, chrysopa septempunctata etc.), and a large amount of expansions that the artificial feeding of natural enemy insect be unable to do without life food are numerous.Acyrthosiphum pisim, because of advantages such as its individuality are comparatively large, fertility is strong, is a kind of theme feed that current natural enemy insect is raised.
This field, laboratory discharges harmonia axyridia in a large number and controls field cigarette aphid, mainly through expanding numerous acyrthosiphum pisim in a large number with the biotroph providing Larva Harmonia Axyridis to grow and needed for Adult worms producting eggs.Broad bean because of its biomass large, the feature of fast growth is the main host plants plant that current acyrthosiphum pisim is propagated artificially.
Because acyrthosiphum pisim belongs to insect, the artificial feeding of insect needs artificial isolation; Precipitation can produce aphid and very strong scrub effect, and propagating artificially of acyrthosiphum pisim must have rainproof facility; Most importantly the continuous plantation of pea causes soil acidification, and then produces the rhizome portion disease of broad bean, makes broad bean wilting, lodging, rotten and death, reduces the reproductive speed of acyrthosiphum pisim.
In order to solve above difficulty, patent disclosure of the present invention one can overcome an intrinsic difficult problem, and effectively can meet acyrthosiphum pisim and expand numerous method in a large number.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of artificial a large amount of method expanding numerous acyrthosiphum pisim.
A kind of artificial a large amount of method expanding numerous acyrthosiphum pisim of the present invention, is characterized in that including following steps:
A. seed treatment: soaked in water by Broad Bean Seeds, in order to sowing;
B. matrix sowing is loaded: in nursery floating plate, load moistening tobacco seedling medium; Select the broad bean particle of full seed, one, seed is inserted in each nursery hole;
C. floating plate enters water: in black water pond, and dress water, adds composite fertilizer in water pond; The nursery floating plate sowed is put into black water pond;
D. to emerge inoculation: sow after 3 days, broad bean is emerged substantially; Sow after 12 days, start to inoculate acyrthosiphum pisim, and cover upper Arch Reticulated canopy at once;
E. aphid is mended and connects: sow after 17 days, every strain broad bean all there will be the little aphid of life of trooping in a large number, if little aphid does not appear in indivedual plant, needs again to mend to connect;
F. clip is fed: sow after 30 days, and acyrthosiphum pisim is covered with the tender position of broad bean children, starts clip and feeds natural enemy insect;
G. post-processed: 5 day time, the tender point of broad bean of the whole water pond of clip, aphid more than needed can be seeded to next group and treat in clip water pond;
H. circulation pattern: broad bean sowing in every 5 days once, starts clip and uses, finish using in 5 days after 30 days.
The invention discloses a kind of artificial a large amount of method expanding numerous acyrthosiphum pisim, it is characterized in that including following steps:
A. seed treatment: Broad Bean Seeds is soaked in water, and air-dry, in order to sowing;
B. matrix sowing is loaded: the namely loose moistening tobacco seedling medium loading the agglomerating pine pinched in nursery floating plate; Select the broad bean particle of full seed, one, seed is inserted in each nursery hole;
C. floating plate enters water: in black water pond, and 50cm is dark for dress water, adds the tobacco compound fertilizer of 20g in water pond; The nursery floating plate sowed is put into the black water pond of liquid manure abundance;
D. to emerge inoculation: sow after 3 days, broad bean is emerged substantially; Sow after 12 days, start to inoculate acyrthosiphum pisim, and cover upper Arch Reticulated canopy at once;
E. aphid is mended and connects: sow after 17 days, every strain broad bean all there will be the little aphid of life of trooping in a large number, if little aphid does not appear in indivedual plant, needs again to mend to connect;
F. clip is fed: sow after 30 days, and acyrthosiphum pisim is covered with the tender position of broad bean children, starts the stem apex that clip broad bean children is tender, feeds natural enemy insect;
G. post-processed: 5 day time, the tender point of broad bean of the whole water pond of clip, aphid more than needed can be seeded to next group and treat in clip water pond; After black water pond and nursery floating plate clear water are rinsed well, with 1 ‰ potassium permanganate sterilizations, dry for subsequent use;
H. circulation pattern: once, start clip after 30 days and use, finish using in 5 days, the sterilization of water pond, floating plate is for subsequent use in broad bean sowing in every 5 days.
The invention discloses a kind of artificial a large amount of method expanding numerous acyrthosiphum pisim, it is characterized in that including following steps: a. seed treatment: Broad Bean Seeds is soaked 24 hours in water, and air-dry 12 hours, in order to sowing;
B. matrix sowing is loaded: the namely loose moistening tobacco seedling medium loading the agglomerating pine pinched in nursery floating plate, consistent with floating plate plane to substrate plane; Select the broad bean particle of full seed, one, seed is inserted in each nursery hole;
C. floating plate enters water: in black water pond, and 50cm is dark for dress water, adds the tobacco compound fertilizer of 20g active ingredient N:P:K=10%:10%:20% in water pond; The nursery floating plate sowed is put into the black water pond of liquid manure abundance;
D. to emerge inoculation: sow after 3 days, broad bean is emerged substantially, and cavities of not emerging on a small quantity needs reseeding to fill the gaps with seedlings; Sow after 12 days, broad bean grows to 10-15cm, starts to inoculate acyrthosiphum pisim, every strain broad bean inoculate 5 advanced age acyrthosiphum pisim, and the at once upper Arch Reticulated canopy of cover;
E. aphid is mended and connects: sow after 17 days, every strain broad bean all there will be the little aphid of life of trooping in a large number, if little aphid does not appear in indivedual plant, needs again to mend to connect;
F. clip is fed: sow after 30 days, broad bean plant height 30cm, and acyrthosiphum pisim is covered with the tender position of broad bean children, and every strain broad bean has acyrthosiphum pisim 200, starts the stem apex of the tender 10cm of clip broad bean children, together with acyrthosiphum pisim, feeds natural enemy insect;
G. post-processed: 5 day time, the tender point of broad bean of the whole water pond of clip, aphid more than needed can be seeded to next group and treat in clip water pond; And at once by remaining broad bean rhizome, seedling medium and residue liquid manure in water pond, be transported in periphery farmland, can be used as fertilizer for agricultural product and nutrition is provided; After black water pond and nursery floating plate clear water are rinsed well, with 1 ‰ potassium permanganate sterilizations, dry for subsequent use;
H. circulation pattern: broad bean sowing in every 5 days once, determines seeding quantity at every turn as required, and start clip after 30 days and use, finish using, bring into use next group broad bean in 5 days, residue consumes goods and materials and clears out foster worm place, and the sterilization of water pond, floating plate is for subsequent use.
The invention discloses a kind of artificial a large amount of method expanding numerous acyrthosiphum pisim, in use note:
1., during sowing, mend the process connecing aphid and the tender point of clip broad bean and try one's best fast, avoid a small amount of acyrthosiphum pisim to climb out of black water pond region, endanger other crops, acyrthosiphum pisim, when food is sufficient, generally there will not be alatae, wingless aphid creep speed is also unhappy, also seldom creeps, and generally can not climb out of.
2. broad bean seedling was finished using in 5 days as far as possible, and brought into use next group.Time is spun out, and rhizome portion disease easily appears in broad bean, grows pathogen.
3. after the tender point of clip broad bean, residue bean plant and seedling medium and residue liquid manure will clear out foster worm place, avoid residual pathogen to cause harm new plant.
4. this pattern operates in temperature is 20-25 degree, carries out in the green house of humidity 50%-80%, and different foster worm conditions needs according to circumstances, adjusts to some extent.
The experimental control situation of the method for the numerous acyrthosiphum pisim of a kind of artificial a large amount of expansion of the present invention is as follows:
Design 8 process altogether, wherein new loam is contrast.Be respectively
(1) T1 new matrix 10 basin * 10 strain,
(2) T2 continuous cropping matrix 10 basin * 10 strain,
(3) T3 new sandy soil 10 basin * 10 strain,
(4) T4 continuous cropping sandy soil 10 basin * 10 strain,
(5) T5 new loam 10 basin * 10 strain,
(6) T6 continuous cropping loam 10 basin * 10 strain,
(7) T7 this patent pattern cultivates hole, floating plate × 153, broad bean 2 water pond × 2,
(8) T8 this patent pattern cultivates hole, floating plate × 153, pea 2 water pond × 2.
Test method: select Broad Bean Seeds and pea seed, water logging is dried for 24 hours, is seeded in respectively in flowerpot or floating plate by processing requirements.Potted plant uses the nutrient solution of 0.1% to water pouring moisturizing every day; The nutrient solution 15 kilograms of 0.1% is injected in each basin.
Experimental condition: artificial constant temperature insectary: temperature 20-25 degree, humidity 60-80%.
Recording method: first 10 days record emergence rates, after 10 days, the plant that the strain of each flowerpot Stochastic choice 1 represents this basin average production level measures plant height, gridiron pattern sampling selection 10 strain in basin, record plant height and the number of sheets.When starting dead, record lethality.
Interpretation of result:
Emergence rate is studied: several planting patterns emergence rate the results are shown in Figure 1, and result shows: relative to other seedling raising mannerses, and this patent seedling-growing time early 3 days, emergence rate are high by 15%.
Growth rate research: Fig. 2 is shown in the impact of different land use patterns on plant strain growth speed, and result shows: relative to other seedling raising mannerses, this patent seedling raising manners plant strain growth speed is faster.
Lethality research: the plantation of indoor broad bean, because of the deficiency of the conditions such as illumination, broad bean, pea all cannot blossom and bear fruit, and grow into certain hour, start lodging, and dead gradually, and details are shown in accompanying drawing 3.As can be seen from accompanying drawing 3, this patent nursery pattern plant lethality is lower, and the death time is more late, and the expansion being more applicable to broad bean growth and acyrthosiphum pisim is numerous.
Acyrthosiphum pisim breeding amount is investigated: under this patent pattern, by five point samplings, investigate the population density of acyrthosiphum pisim on broad bean every day.Institute an inquiry from sowing acyrthosiphum pisim inoculation in 12 days, substantially constant to pea aphid population density, alatae starts (alatae means that population density is substantially saturated) occurs, and investigates 25 days altogether.Result shows: when 30 days, and aphid amount draw, greatly about 200 heads/plant, reaches the period of great prosperity substantially, and after 30 days, aphid amount does not continue to increase, and alatae starts to expand numerous in addition, and display population density reaches capacity, and population differentiation alatae is to find new food source.Therefore, under 20-25 degree condition, after 30 days, acyrthosiphum pisim is expanded numerous saturated to density, need use as early as possible.Refer to accompanying drawing 4.
The method that the present invention manually expands numerous acyrthosiphum pisim in a large number belongs to insect green prevention and control field, relates to a large amount of mass rearing methods of Live food insect, is applicable to the artificial feeding of natural enemy insect.
Accompanying drawing explanation
Accompanying drawing 1 is that different planting patterns is on the impact of plant emergence rate;
Accompanying drawing 2 is the impact of different land use patterns on plant strain growth speed;
Accompanying drawing 3 is the lethality of plant under different land use patterns;
Accompanying drawing 4 is the population density of plant under different land use patterns.
In accompanying drawing: T1-is new matrix; T2-is continuous cropping matrix; T3-is new sandy soil; T4-is continuous cropping sandy soil; T5-is new loam; T6-is continuous cropping loam; T7-is this patent mode treatment broad bean; T8-is this patent mode treatment pea.
Embodiment
Embodiment 1: the present invention manually expands the method for numerous acyrthosiphum pisim in a large number, includes following steps:
A. seed treatment: Broad Bean Seeds is soaked 24 hours in water, and air-dry 12 hours, in order to sowing;
B. matrix sowing is loaded: the namely loose moistening tobacco seedling medium loading the agglomerating pine pinched in nursery floating plate, consistent with floating plate plane to substrate plane; Select the broad bean particle of full seed, one, seed is inserted in each nursery hole;
C. floating plate enters water: in black water pond, and 50cm is dark for dress water, adds the tobacco compound fertilizer of 20g active ingredient N:P:K=10%:10%:20% in water pond; The nursery floating plate sowed is put into the black water pond of liquid manure abundance;
D. to emerge inoculation: sow after 3 days, broad bean is emerged substantially, and cavities of not emerging on a small quantity needs reseeding to fill the gaps with seedlings; Sow after 12 days, broad bean grows to 10-15cm, starts to inoculate acyrthosiphum pisim, every strain broad bean inoculate 5 advanced age acyrthosiphum pisim, and the at once upper Arch Reticulated canopy of cover;
E. aphid is mended and connects: sow after 17 days, every strain broad bean all there will be the little aphid of life of trooping in a large number, if little aphid does not appear in indivedual plant, needs again to mend to connect;
F. clip is fed: sow after 30 days, broad bean plant height 30cm, and acyrthosiphum pisim is covered with the tender position of broad bean children, and every strain broad bean has acyrthosiphum pisim 200, starts the stem apex of the tender 10cm of clip broad bean children, together with acyrthosiphum pisim, feeds natural enemy insect;
G. post-processed: 5 day time, the tender point of broad bean of the whole water pond of clip, aphid more than needed can be seeded to next group and treat in clip water pond; And at once by remaining broad bean rhizome, seedling medium and residue liquid manure in water pond, be transported in periphery farmland, can be used as fertilizer for agricultural product and nutrition is provided; After black water pond and nursery floating plate clear water are rinsed well, with 1 ‰ potassium permanganate sterilizations, dry for subsequent use;
H. circulation pattern: broad bean sowing in every 5 days once, determines seeding quantity at every turn as required, and start clip after 30 days and use, finish using, bring into use next group broad bean in 5 days, residue consumes goods and materials and clears out foster worm place, and the sterilization of water pond, floating plate is for subsequent use.
In embodiment 2:5 month, now field aphid starts to occur, and predator needs a large amount of expansion numerous, belongs to amplification aquaculture model.Natural enemy insect amount reproduction, quantity sharply increases, and each nursery increases a floating plate, until seedling culture space taken, then enters the numerous pattern of stable expansion.Under amplification aquaculture model, food needs amount continues to increase, and keeps 6 batches of nursery, circulation in 30 days.Every a collection of seedling provides the food of 5 days, is divided into 5 parts by broad bean seedling, clip portion every day (only clip climbs the stem apex of full acyrthosiphum pisim), puts into natural enemy insect insect box.If acyrthosiphum pisim uses not, natural enemy insect by survival of the fittest natural selection, can ensure making full use of of limited food.
Embodiment 3:8 month, aphid occurs in a large number in field, natural enemy insect full production, belongs to stable and expands numerous pattern, during this period, seedling culture space running at full capacity, nursery amount is substantially constant, and acyrthosiphum pisim output also remains unchanged substantially, still keeps 6 batches of nursery, circulation in 30 days, every a collection of seedling provides the food of 5 days.When acyrthosiphum pisim cannot provide enough food, unnecessary natural enemy insect is released to field, ensures that the expansion of the production of all the other insects is numerous.
In embodiment 4:10 month, field aphis population density is lower, no longer needs the field adaptability of natural enemy insect, and the production of natural enemy insect belongs to Breed conservation model.Still keep 6 batches of nursery, often criticize nursery and only sow a dish, circulation in 30 days, each dish seedling provides the food of 5 days.Acyrthosiphum pisim deficiency uses, and by natural selection and the survival of the fittest of natural enemy insect, ensures the continuity of population.
Claims (3)
1. the artificial a large amount of method expanding numerous acyrthosiphum pisim, is characterized in that including following steps:
A. seed treatment: soaked in water by Broad Bean Seeds, in order to sowing;
B. matrix sowing is loaded: in nursery floating plate, load moistening tobacco seedling medium; Select the broad bean particle of full seed, one, seed is inserted in each nursery hole;
C. floating plate enters water: in black water pond, and dress water, adds composite fertilizer in water pond; The nursery floating plate sowed is put into black water pond;
D. to emerge inoculation: sow after 3 days, broad bean is emerged substantially; Sow after 12 days, start to inoculate acyrthosiphum pisim, and cover upper Arch Reticulated canopy at once;
E. aphid is mended and connects: sow after 17 days, every strain broad bean all there will be the little aphid of life of trooping in a large number, if little aphid does not appear in indivedual plant, needs again to mend to connect;
F. clip is fed: sow after 30 days, and acyrthosiphum pisim is covered with the tender position of broad bean children, starts clip and feeds natural enemy insect;
G. post-processed: 5 day time, the tender point of broad bean of the whole water pond of clip, aphid more than needed can be seeded to next group and treat in clip water pond;
H. circulation pattern: broad bean sowing in every 5 days once, starts clip and uses, finish using in 5 days after 30 days.
2., according to artificial a large amount of method expanding numerous acyrthosiphum pisim according to claim 1, it is characterized in that including following steps:
A. seed treatment: Broad Bean Seeds is soaked in water, and air-dry, in order to sowing;
B. matrix sowing is loaded: the namely loose moistening tobacco seedling medium loading the agglomerating pine pinched in nursery floating plate; Select the broad bean particle of full seed, one, seed is inserted in each nursery hole;
C. floating plate enters water: in black water pond, and 50cm is dark for dress water, adds the tobacco compound fertilizer of 20g in water pond; The nursery floating plate sowed is put into the black water pond of liquid manure abundance;
D. to emerge inoculation: sow after 3 days, broad bean is emerged substantially; Sow after 12 days, start to inoculate acyrthosiphum pisim, and cover upper Arch Reticulated canopy at once;
E. aphid is mended and connects: sow after 17 days, every strain broad bean all there will be the little aphid of life of trooping in a large number, if little aphid does not appear in indivedual plant, needs again to mend to connect;
F. clip is fed: sow after 30 days, and acyrthosiphum pisim is covered with the tender position of broad bean children, starts the stem apex that clip broad bean children is tender, feeds natural enemy insect;
G. post-processed: 5 day time, the tender point of broad bean of the whole water pond of clip, aphid more than needed can be seeded to next group and treat in clip water pond; After black water pond and nursery floating plate clear water are rinsed well, with 1 ‰ potassium permanganate sterilizations, dry for subsequent use;
H. circulation pattern: once, start clip after 30 days and use, finish using in 5 days, the sterilization of water pond, floating plate is for subsequent use in broad bean sowing in every 5 days.
3., according to artificial a large amount of method expanding numerous acyrthosiphum pisim according to claim 1, it is characterized in that including following steps: a. seed treatment: Broad Bean Seeds is soaked 24 hours in water, and air-dry 12 hours, in order to sowing;
B. matrix sowing is loaded: the namely loose moistening tobacco seedling medium loading the agglomerating pine pinched in nursery floating plate, consistent with floating plate plane to substrate plane; Select the broad bean particle of full seed, one, seed is inserted in each nursery hole;
C. floating plate enters water: in black water pond, and 50cm is dark for dress water, adds the tobacco compound fertilizer of 20g active ingredient N:P:K=10%:10%:20% in water pond; The nursery floating plate sowed is put into the black water pond of liquid manure abundance;
D. to emerge inoculation: sow after 3 days, broad bean is emerged substantially, and cavities of not emerging on a small quantity needs reseeding to fill the gaps with seedlings; Sow after 12 days, broad bean grows to 10-15cm, starts to inoculate acyrthosiphum pisim, every strain broad bean inoculate 5 advanced age acyrthosiphum pisim, and the at once upper Arch Reticulated canopy of cover;
E. aphid is mended and connects: sow after 17 days, every strain broad bean all there will be the little aphid of life of trooping in a large number, if little aphid does not appear in indivedual plant, needs again to mend to connect;
F. clip is fed: sow after 30 days, broad bean plant height 30cm, and acyrthosiphum pisim is covered with the tender position of broad bean children, and every strain broad bean has acyrthosiphum pisim 200, starts the stem apex of the tender 10cm of clip broad bean children, together with acyrthosiphum pisim, feeds natural enemy insect;
G. post-processed: 5 day time, the tender point of broad bean of the whole water pond of clip, aphid more than needed can be seeded to next group and treat in clip water pond; And at once by remaining broad bean rhizome, seedling medium and residue liquid manure in water pond, be transported in periphery farmland, can be used as fertilizer for agricultural product and nutrition is provided; After black water pond and nursery floating plate clear water are rinsed well, with 1 ‰ potassium permanganate sterilizations, dry for subsequent use;
H. circulation pattern: broad bean sowing in every 5 days once, determines seeding quantity at every turn as required, and start clip after 30 days and use, finish using, bring into use next group broad bean in 5 days, residue consumes goods and materials and clears out foster worm place, and the sterilization of water pond, floating plate is for subsequent use.
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Cited By (6)
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| CN105519496A (en) * | 2016-02-16 | 2016-04-27 | 弥渡格瑞生物科技有限责任公司 | Natural diffusion myzus persicae vaccination method |
| CN105918257A (en) * | 2016-04-14 | 2016-09-07 | 伊犁哈萨克自治州林业科学研究院 | Method for artificially breeding Sclerodermus pupariae by using local alternate hosts |
| CN108174824A (en) * | 2018-01-31 | 2018-06-19 | 南阳师范学院 | A kind of low-density cultural method for keeping the lonely female aptery state of aphid of acyrthosiphum pisim |
| CN109247303A (en) * | 2018-11-16 | 2019-01-22 | 湖北省农业科学院粮食作物研究所 | Utilize the method for water planting semen viciae fabae seedling mass propagation bean aphid |
| CN110692602A (en) * | 2019-11-13 | 2020-01-17 | 北京阔野田园生物技术有限公司 | Method for large-scale production of pea aphid workshop |
| CN112243770A (en) * | 2020-10-20 | 2021-01-22 | 四川省烟草公司凉山州公司 | Method for preventing myzus persicae by using field cultivation system |
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| CN105519496A (en) * | 2016-02-16 | 2016-04-27 | 弥渡格瑞生物科技有限责任公司 | Natural diffusion myzus persicae vaccination method |
| CN105519496B (en) * | 2016-02-16 | 2017-11-21 | 弥渡格瑞生物科技有限责任公司 | A kind of natural diffuseness of cigarette aphid connects aphid method |
| CN105918257A (en) * | 2016-04-14 | 2016-09-07 | 伊犁哈萨克自治州林业科学研究院 | Method for artificially breeding Sclerodermus pupariae by using local alternate hosts |
| CN108174824A (en) * | 2018-01-31 | 2018-06-19 | 南阳师范学院 | A kind of low-density cultural method for keeping the lonely female aptery state of aphid of acyrthosiphum pisim |
| CN108174824B (en) * | 2018-01-31 | 2020-11-13 | 南阳师范学院 | Low-density breeding method for keeping wingless state of pea aphid parthenogenetic female aphid |
| CN109247303A (en) * | 2018-11-16 | 2019-01-22 | 湖北省农业科学院粮食作物研究所 | Utilize the method for water planting semen viciae fabae seedling mass propagation bean aphid |
| CN110692602A (en) * | 2019-11-13 | 2020-01-17 | 北京阔野田园生物技术有限公司 | Method for large-scale production of pea aphid workshop |
| CN112243770A (en) * | 2020-10-20 | 2021-01-22 | 四川省烟草公司凉山州公司 | Method for preventing myzus persicae by using field cultivation system |
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