CN104640977B - 重组微生物制备生物柴油 - Google Patents

重组微生物制备生物柴油 Download PDF

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Publication number
CN104640977B
CN104640977B CN201380044451.9A CN201380044451A CN104640977B CN 104640977 B CN104640977 B CN 104640977B CN 201380044451 A CN201380044451 A CN 201380044451A CN 104640977 B CN104640977 B CN 104640977B
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clostridium
microorganism
nucleic acid
biodiesel
bacterium
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Chinese (zh)
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CN104640977A (zh
Inventor
F·刘
M·科普克
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Lanzatech NZ Inc
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Lanzatech New Zealand Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/649Biodiesel, i.e. fatty acid alkyl esters
    • CCHEMISTRY; METALLURGY
    • C10PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10LFUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00Liquid carbonaceous fuels
    • C10L1/02Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102Diacylglycerol O-acyltransferase (2.3.1.20)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01075Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145Clostridium
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380044451.9A 2012-06-21 2013-06-21 重组微生物制备生物柴油 Active CN104640977B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261662467P 2012-06-21 2012-06-21
US61/662,467 2012-06-21
PCT/NZ2013/000108 WO2013191567A1 (en) 2012-06-21 2013-06-21 Recombinant microorganisms make biodiesel

Publications (2)

Publication Number Publication Date
CN104640977A CN104640977A (zh) 2015-05-20
CN104640977B true CN104640977B (zh) 2019-04-12

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ID=49769067

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CN201380044451.9A Active CN104640977B (zh) 2012-06-21 2013-06-21 重组微生物制备生物柴油

Country Status (13)

Country Link
US (1) US9347076B2 (enExample)
EP (1) EP2864470B2 (enExample)
JP (1) JP6466836B2 (enExample)
KR (1) KR102097844B1 (enExample)
CN (1) CN104640977B (enExample)
BR (1) BR112014031876B1 (enExample)
CA (1) CA2876178C (enExample)
ES (1) ES2655214T5 (enExample)
IN (1) IN2014DN10507A (enExample)
MY (1) MY164882A (enExample)
NO (1) NO2953671T3 (enExample)
PL (1) PL2864470T5 (enExample)
WO (1) WO2013191567A1 (enExample)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201112231D0 (en) 2011-07-15 2011-08-31 Verdant Bioproducts Ltd Micro-organism
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel
SG11201605775UA (en) 2014-01-28 2016-08-30 Lanzatech New Zealand Ltd Method of producing a recombinant microorganism
JP6309304B2 (ja) * 2014-02-24 2018-04-11 積水化学工業株式会社 組換え細胞、並びに、1,4−ブタンジオールの生産方法
US9834792B2 (en) 2014-10-22 2017-12-05 Lanzatech New Zealand Limited Multi-stage bioreactor processes
EP3210012B1 (en) 2014-10-22 2023-06-07 LanzaTech NZ, Inc. Gas testing unit and method
WO2016138050A1 (en) 2015-02-23 2016-09-01 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
JP6871870B2 (ja) * 2015-05-27 2021-05-19 ランザテク・ニュージーランド・リミテッド コリスミ酸誘導産物生成のための遺伝子組換え微生物(関連出願の相互参照)
MX2023003058A (es) 2015-10-13 2023-04-05 Lanzatech Nz Inc Bacteria diseñada por ingeniería genética que comprende una trayectoria de fermentación que genera energía.
DK3384005T3 (da) 2015-12-03 2022-04-11 Lanzatech Nz Inc Arginin som eneste nitrogenkilde til C1-fikserende mikroorganisme
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
WO2017136478A1 (en) 2016-02-01 2017-08-10 Lanzatech New Zealand Limited Integrated fermentation and electrolysis process
CA3015665C (en) 2016-02-26 2020-09-22 Lanzatech New Zealand Limited Crispr/cas systems for c1-fixing bacteria
SG11201810031RA (en) 2016-05-14 2018-12-28 Lanzatech Inc Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
CN107217017B (zh) * 2017-05-27 2020-12-08 青岛农业大学 一株不动杆菌及其在石油降解中的应用
EP3679149B1 (en) 2017-09-08 2022-06-29 Lanzatech, Inc. Processes for metabolite production using hydrogen rich c1-containing substrates
AU2018393075B2 (en) 2017-12-19 2024-03-21 Lanzatech, Inc. Microorganisms and methods for the biological production of ethylene glycol
US11359294B2 (en) 2018-02-12 2022-06-14 Lanzatech, Inc. Process for improving carbon conversion efficiency
MY204224A (en) 2018-04-20 2024-08-16 Lanzatech Inc Intermittent electrolysis streams
MY199603A (en) 2018-11-19 2023-11-08 Lanzatech Inc Integration of fermentation and gasification
US11091415B2 (en) 2019-02-08 2021-08-17 Lanzatech, Inc. Process for recovering close boiling products
CN112955560A (zh) 2019-07-11 2021-06-11 朗泽科技有限公司 优化气体利用的方法
CN111088193B (zh) * 2020-01-10 2022-09-09 南京工业大学 一种提高食甲基丁酸杆菌电转频率的方法
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
CA3171681A1 (en) 2020-03-18 2021-09-23 Fungmin Liew Fermentative production of 2-phenylethanol from gaseous substrates
BR112022021838A2 (pt) 2020-04-29 2022-12-13 Lanzatech Inc Microrganismo, método para produzir ss-cetoadipato, e, método de produção de um polímero
BR112022024652B1 (pt) 2020-06-06 2024-01-16 Lanzatech, Inc Micro-organismo geneticamente modificado, e, método para a produção de um produto
WO2022170191A1 (en) 2021-02-08 2022-08-11 Lanzatech, Inc. Recombinant microorganisms and uses therefor
BR112023020761A2 (pt) 2021-04-09 2023-12-12 Lanzatech Inc Processo para fornecer uma matéria-prima, e, aparelho para fornecer uma matéria-prima de fermentação gasosa
US12241105B2 (en) 2021-07-20 2025-03-04 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
JP2025525381A (ja) 2022-06-21 2025-08-05 ランザテク,インコーポレイテッド C1基質からの高価値の特殊タンパク質及び化学産物の連続共産生のための微生物及び方法
JP2025525373A (ja) 2022-06-21 2025-08-05 ランザテク,インコーポレイテッド C1-基質からのタンデムリピートタンパク質及び化学産物の連続共産生のための微生物及び方法
US12129503B2 (en) 2022-08-10 2024-10-29 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
WO2024253883A1 (en) 2023-06-05 2024-12-12 Lanzatech, Inc. Integrated gas fermentation and carbon black processes
US12281344B2 (en) 2023-06-05 2025-04-22 Lanzatech, Inc. Integrated gas fermentation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1444658A (zh) * 2000-07-25 2003-09-24 生物工程资源股份有限公司 提高微生物发酵生产乙醇的方法
CN101680009A (zh) * 2007-03-28 2010-03-24 Ls9公司 提高脂肪酸衍生物的产量
WO2012026833A1 (en) * 2010-08-26 2012-03-01 Lanzatech New Zealand Limited Process for producing ethanol and ethylene via fermentation
CN102459569A (zh) * 2009-04-10 2012-05-16 Ls9公司 脂肪酸衍生物的产生
CN102492672A (zh) * 2011-11-26 2012-06-13 宁波大学 一种催化二酰甘油酰基化的酶

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
DE102004052115A1 (de) * 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
ES2528482T3 (es) * 2006-05-19 2015-02-10 Ls9, Inc. Producción de ácidos grasos y derivados de los mismos
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
WO2009009391A2 (en) * 2007-07-06 2009-01-15 Ls9, Inc. Systems and methods for the production of fatty esters
EA022710B1 (ru) 2007-11-13 2016-02-29 Ланзатек Нью Зиленд Лимитед Штамм бактерии clostridium autoethanogenum, способный продуцировать этанол и ацетат путем анаэробной ферментации субстрата, содержащего co
GB0801787D0 (en) * 2008-01-31 2008-03-05 Reclaim Resources Ltd Apparatus and method for treating waste
US8048654B2 (en) * 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
WO2010022090A1 (en) * 2008-08-18 2010-02-25 Ls9, Inc. Systems and methods for the production of mixed fatty esters
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
US20110236941A1 (en) 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
TWI541348B (zh) 2011-02-25 2016-07-11 藍瑟科技紐西蘭有限公司 重組一氧化碳營養性產乙酸微生物及其用途
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1444658A (zh) * 2000-07-25 2003-09-24 生物工程资源股份有限公司 提高微生物发酵生产乙醇的方法
CN101680009A (zh) * 2007-03-28 2010-03-24 Ls9公司 提高脂肪酸衍生物的产量
CN102459569A (zh) * 2009-04-10 2012-05-16 Ls9公司 脂肪酸衍生物的产生
WO2012026833A1 (en) * 2010-08-26 2012-03-01 Lanzatech New Zealand Limited Process for producing ethanol and ethylene via fermentation
CN102492672A (zh) * 2011-11-26 2012-06-13 宁波大学 一种催化二酰甘油酰基化的酶

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Clostridium ljungdahlii represents a microbial production platform based on syngas;Michael Köpke et al.;《PNAS》;20100824;摘要 *
Microdiesel: Escherichia coli engineered for fuel production;R. Kalscheuer et al.;《Microbiology》;20061231;第2530页右栏第一段最后5行 *

Also Published As

Publication number Publication date
CN104640977A (zh) 2015-05-20
ES2655214T5 (es) 2021-10-11
JP2015519918A (ja) 2015-07-16
KR20150023651A (ko) 2015-03-05
ES2655214T3 (es) 2018-02-19
CA2876178C (en) 2017-02-28
JP6466836B2 (ja) 2019-02-06
US9347076B2 (en) 2016-05-24
EP2864470B2 (en) 2020-12-23
BR112014031876A2 (pt) 2017-08-01
PL2864470T3 (pl) 2018-05-30
WO2013191567A1 (en) 2013-12-27
NO2953671T3 (enExample) 2018-03-17
US20130344547A1 (en) 2013-12-26
KR102097844B1 (ko) 2020-04-06
EP2864470A1 (en) 2015-04-29
BR112014031876B1 (pt) 2022-05-10
EP2864470B1 (en) 2017-10-04
CA2876178A1 (en) 2013-12-27
IN2014DN10507A (enExample) 2015-08-21
PL2864470T5 (pl) 2021-05-17
MY164882A (en) 2018-01-30
EP2864470A4 (en) 2015-12-16

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