CN104619835B - 酮醇酸还原异构酶及其使用方法 - Google Patents

酮醇酸还原异构酶及其使用方法 Download PDF

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Publication number
CN104619835B
CN104619835B CN201380036539.6A CN201380036539A CN104619835B CN 104619835 B CN104619835 B CN 104619835B CN 201380036539 A CN201380036539 A CN 201380036539A CN 104619835 B CN104619835 B CN 104619835B
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seq
host cell
gene
polypeptide
kari
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CN104619835A (zh
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S.戈维达拉詹
Y.李
D-I.廖
D.P.奧基菲
J.S.明舒尔
S.C.罗斯曼
A.V.托比亚斯
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Butamax Advanced Biofuels LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01086—Ketol-acid reductoisomerase (1.1.1.86)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380036539.6A 2012-05-11 2013-05-10 酮醇酸还原异构酶及其使用方法 Expired - Fee Related CN104619835B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261645832P 2012-05-11 2012-05-11
US61/645832 2012-05-11
US201361787480P 2013-03-15 2013-03-15
US61/787480 2013-03-15
PCT/US2013/040627 WO2013176909A2 (en) 2012-05-11 2013-05-10 Ketol-acid reductoisomerase enzymes and methods of use

Publications (2)

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CN104619835A CN104619835A (zh) 2015-05-13
CN104619835B true CN104619835B (zh) 2018-06-19

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US (2) US9169467B2 (cg-RX-API-DMAC7.html)
EP (1) EP2847326A2 (cg-RX-API-DMAC7.html)
JP (1) JP6321631B2 (cg-RX-API-DMAC7.html)
KR (1) KR20150014953A (cg-RX-API-DMAC7.html)
CN (1) CN104619835B (cg-RX-API-DMAC7.html)
AU (1) AU2013266727A1 (cg-RX-API-DMAC7.html)
BR (1) BR112014028153A2 (cg-RX-API-DMAC7.html)
CA (1) CA2873109A1 (cg-RX-API-DMAC7.html)
MX (2) MX2014013446A (cg-RX-API-DMAC7.html)
NZ (1) NZ701099A (cg-RX-API-DMAC7.html)
WO (1) WO2013176909A2 (cg-RX-API-DMAC7.html)
ZA (1) ZA201407713B (cg-RX-API-DMAC7.html)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
EP2543721A1 (en) 2007-04-18 2013-01-09 Butamax (TM) Advanced Biofuels LLC Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
AU2009296224B2 (en) 2008-09-29 2015-02-12 Butamax(Tm) Advanced Biofuels Llc Increased heterologous Fe-S enzyme actiivty in yeast
MX2012007583A (es) 2009-12-29 2012-07-30 Butamax Tm Advanced Biofuels Alcohol deshidrogenasas (adh) utiles para la produccion fermentativa de alcoholes alquilicos de cadena corta.
CA2788842C (en) 2010-02-17 2019-03-19 Butamax(Tm) Advanced Biofuels Llc Improving activity of fe-s cluster requiring proteins
CN103080298A (zh) 2010-09-07 2013-05-01 布特马斯先进生物燃料有限责任公司 整合编码催化丙酮酸至乙酰乳酸的转化的多肽的多核苷酸
AU2012230737B2 (en) 2011-03-24 2017-02-23 Butamax (Tm) Advanced Biofuels Llc Host cells and methods for production of isobutanol
KR20140099224A (ko) 2011-07-28 2014-08-11 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 케토-아이소발레레이트 데카르복실라제 효소 및 이의 이용 방법
EP2798072A2 (en) 2011-12-30 2014-11-05 Butamax Advanced Biofuels LLC Fermentative production of alcohols
CN104284981A (zh) 2012-03-23 2015-01-14 布特马斯先进生物燃料有限责任公司 在针对产丁醇生物的培养基中补充乙酸盐
CN104619835B (zh) 2012-05-11 2018-06-19 布特马斯先进生物燃料有限责任公司 酮醇酸还原异构酶及其使用方法
US9593349B2 (en) 2012-08-22 2017-03-14 Butamax Advanced Biofuels Llc Fermentative production of alcohols
US9840724B2 (en) 2012-09-21 2017-12-12 Butamax Advanced Biofuels Llc Production of renewable hydrocarbon compositions
CA2886157A1 (en) 2012-09-26 2014-04-03 Butamax Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
CA2905912A1 (en) 2013-03-14 2014-10-02 E. I. Du Pont De Nemours And Company Glycerol 3- phosphate dehydrogenase for butanol production
US9580705B2 (en) 2013-03-15 2017-02-28 Butamax Advanced Biofuels Llc DHAD variants and methods of screening
US9771602B2 (en) 2013-03-15 2017-09-26 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
CA2957920A1 (en) 2014-08-11 2016-02-18 Butamax Advanced Biofuels Llc Yeast preparations and methods of making the same
CN110177801B (zh) 2016-11-09 2024-02-23 丹尼斯科美国公司 具有改善的醇生产的酵母
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
KR101960501B1 (ko) * 2017-09-15 2019-03-21 고려대학교 산학협력단 Ｎａｄｈ 친화적인 신규 케톨산 리덕토아이소머라제
US11308274B2 (en) 2019-05-17 2022-04-19 International Business Machines Corporation Word grouping using a plurality of models
CN113278568B (zh) * 2020-05-27 2022-10-21 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组大肠杆菌及其应用
EP4674950A1 (en) * 2023-03-30 2026-01-07 Anhui Huaheng Biotechnology Co., Ltd. Recombinant microorganism for producing pantoic acid and use thereof
CN117417949B (zh) * 2023-10-19 2024-12-20 云南农业大学 雪胆细胞色素氧化酶HcCYP81Q58转基因酵母工程菌及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101297042A (zh) * 2005-10-26 2008-10-29 纳幕尔杜邦公司 四碳醇的发酵生产

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4865973A (en) 1985-09-13 1989-09-12 Queen's University At Kingston Process for extractive fermentation
FR2696190B1 (fr) 1992-09-25 1994-12-09 Agronomique Inst Nat Rech Acide nucléique codant pour une alpha-acétolactate synthase et ses applications.
DE60029971T2 (de) 1999-08-18 2007-05-16 E.I. Dupont De Nemours And Co., Wilmington Verfahren zur biologischen herstellung von 1,3-propandiol
US20020061569A1 (en) 2000-03-21 2002-05-23 Robert Haselbeck Identification of essential genes in prokaryotes
US6586229B1 (en) 2000-06-01 2003-07-01 North Carolina State University Method for the production of ρ-Hydroxybenzoate in species of pseudomonas and agrobacterium
IL159368A0 (en) 2001-07-02 2004-06-01 Kaneka Corp Method of modifying enzyme and oxidoreductase variant
ES2398216T3 (es) 2003-03-10 2013-03-14 Poet Research, Inc. Método para producir etanol usando almidón sin procesar
SE526429C2 (sv) 2003-10-24 2005-09-13 Swedish Biofuels Ab Metod för att framställa syreinnehållande föreningar utgående från biomassa
EP1869197A2 (en) 2005-04-12 2007-12-26 E.I. Dupont De Nemours And Company Treatment of biomass to obtain ethanol
US9297028B2 (en) 2005-09-29 2016-03-29 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US9303225B2 (en) 2005-10-26 2016-04-05 Butamax Advanced Biofuels Llc Method for the production of isobutanol by recombinant yeast
US8273558B2 (en) * 2005-10-26 2012-09-25 Butamax(Tm) Advanced Biofuels Llc Fermentive production of four carbon alcohols
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
US8206970B2 (en) 2006-05-02 2012-06-26 Butamax(Tm) Advanced Biofuels Llc Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
US8828704B2 (en) 2006-05-02 2014-09-09 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US7659104B2 (en) 2006-05-05 2010-02-09 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
US7541173B2 (en) 2006-06-15 2009-06-02 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
US8017364B2 (en) 2006-12-12 2011-09-13 Butamax(Tm) Advanced Biofuels Llc Solvent tolerant microorganisms
MY147186A (en) 2007-02-09 2012-11-14 Univ California Biofuel production by recombinant microorganisms
EP2543721A1 (en) 2007-04-18 2013-01-09 Butamax (TM) Advanced Biofuels LLC Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
EP2060632A1 (en) 2007-10-29 2009-05-20 Technische Universität Berlin Method of modifying a yeast cell for the production of ethanol
WO2009059254A2 (en) 2007-10-31 2009-05-07 Gevo, Inc Methods for the economical production of biofuel precursor that is also a biofuel from biomass
JP2010503379A (ja) 2007-12-17 2010-02-04 サントリーホールディングス株式会社 変異ｉｌｖ５遺伝子及びその用途
EP2222841B1 (en) * 2007-12-20 2015-08-26 Butamax (TM) Advanced Biofuels LLC Ketol-acid reductoisomerase using nadh
US8372612B2 (en) 2007-12-21 2013-02-12 Butamax(Tm) Advanced Biofuels Llc Production of four carbon alcohols using improved strain
US8518678B2 (en) 2007-12-21 2013-08-27 Butamax(Tm) Advanced Biofuels Llc Strain comprising increased expression of a CFA coding region for butanol production
CA2990939A1 (en) 2007-12-23 2009-07-09 Gevo, Inc. Yeast organism producing isobutanol at a high yield
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
US8389252B2 (en) 2008-05-12 2013-03-05 Butamax(Tm) Advanced Biofuels Llc Yeast strain for production of four carbon alcohols
US8906666B2 (en) 2008-05-22 2014-12-09 Butamax Advanced Biofuels Llc Engineering resistance to aliphatic alcohols
BRPI0909989A2 (pt) 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
AU2009296218A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
EP2337851B1 (en) 2008-09-29 2014-08-13 Butamax Advanced Biofuels Llc IDENTIFICATION AND USE OF BACTERIAL Ý2Fe-2S¨DIHYDROXY-ACID DEHYDRATASES
WO2010037119A1 (en) 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria
AU2009296224B2 (en) 2008-09-29 2015-02-12 Butamax(Tm) Advanced Biofuels Llc Increased heterologous Fe-S enzyme actiivty in yeast
EP2337869B1 (en) 2008-09-29 2015-08-19 Butamax Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
WO2010062597A1 (en) 2008-10-27 2010-06-03 Butamax™ Advanced Biofuels LLC Carbon pathway optimized production hosts for the production of isobutanol
US20100143997A1 (en) 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions
US8465964B2 (en) 2008-11-13 2013-06-18 Butamax (TM) Advanced Biofules LLC Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
US8828694B2 (en) 2008-11-13 2014-09-09 Butamax Advanced Biofuels Llc Production of isobutanol in yeast mitochondria
US8652823B2 (en) 2008-12-03 2014-02-18 Butamax(Tm) Advanced Biofuels Llc Strain for butanol production with increased membrane unsaturated trans fatty acids
US8557562B2 (en) 2008-12-29 2013-10-15 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving filamentous growth response
US8795992B2 (en) 2008-12-29 2014-08-05 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving cell wall integrity pathway
US8455225B2 (en) 2008-12-29 2013-06-04 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
US20120058541A1 (en) 2009-05-22 2012-03-08 Butamax(Tm) Advanced Biofuels Llc Engineering resistance to aliphatic alcohols
EP2464736A4 (en) 2009-08-12 2013-07-31 Gevo Inc PATIENT LOCALIZATION OF CYTOSOLIC ISOBUTANOL FOR THE MANUFACTURE OF ISOBUTANOL
EP2483400A1 (en) 2009-09-29 2012-08-08 Butamax(tm) Advanced Biofuels LLC Improved flux to acetolactate-derived products in lactic acid bacteria
CA2775892A1 (en) 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Fermentive production of isobutanol using highly effective ketol-acid reductoisomerase enzymes
EP2483401B1 (en) 2009-09-29 2017-06-21 Butamax (TM) Advanced Biofuels LLC Improved yeast production host cells
US20110195505A1 (en) 2009-10-08 2011-08-11 Butamax(Tm) Advanced Biofuels Llc Bacterial strains for butanol production
MY156003A (en) 2009-11-24 2015-12-31 Gevo Inc Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
CA2785660A1 (en) 2009-12-29 2011-07-07 Butamax(Tm) Advanced Biofuels Llc Expression of hexose kinase in recombinant host cells
MX2012007583A (es) 2009-12-29 2012-07-30 Butamax Tm Advanced Biofuels Alcohol deshidrogenasas (adh) utiles para la produccion fermentativa de alcoholes alquilicos de cadena corta.
RU2012138943A (ru) 2010-02-12 2014-03-20 Джево, Инк. Дрожжевые микроорганизмы с пониженным накоплением побочных продуктов для улучшенного производства топлив, химикатов и аминокислот
CA2788842C (en) 2010-02-17 2019-03-19 Butamax(Tm) Advanced Biofuels Llc Improving activity of fe-s cluster requiring proteins
CA2801576A1 (en) 2010-06-17 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Yeast production culture for the production of butanol
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
CN103080298A (zh) * 2010-09-07 2013-05-01 布特马斯先进生物燃料有限责任公司 整合编码催化丙酮酸至乙酰乳酸的转化的多肽的多核苷酸
AU2012230737B2 (en) 2011-03-24 2017-02-23 Butamax (Tm) Advanced Biofuels Llc Host cells and methods for production of isobutanol
US20120258873A1 (en) 2011-04-06 2012-10-11 Butamax(Tm) Advanced Biofuels Llc Reduction of 2,3-dihydroxy-2-methyl butyrate (dhmb) in butanol production
US20130035515A1 (en) 2011-06-17 2013-02-07 Butamax(Tm) Advanced Biofuels Llc Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation
KR20140099224A (ko) 2011-07-28 2014-08-11 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 케토-아이소발레레이트 데카르복실라제 효소 및 이의 이용 방법
EP2798072A2 (en) 2011-12-30 2014-11-05 Butamax Advanced Biofuels LLC Fermentative production of alcohols
BR112014016005A8 (pt) 2011-12-30 2017-07-04 Butamax Advanced Biofuels Llc alterações genéticas para produção de butanol
CN104284981A (zh) 2012-03-23 2015-01-14 布特马斯先进生物燃料有限责任公司 在针对产丁醇生物的培养基中补充乙酸盐
CN104619835B (zh) 2012-05-11 2018-06-19 布特马斯先进生物燃料有限责任公司 酮醇酸还原异构酶及其使用方法
CA2886157A1 (en) 2012-09-26 2014-04-03 Butamax Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
US20140186911A1 (en) 2012-12-28 2014-07-03 Butamax Advanced Biofuels Llc Recombinant host cells and methods for producing butanol
CA2905912A1 (en) 2013-03-14 2014-10-02 E. I. Du Pont De Nemours And Company Glycerol 3- phosphate dehydrogenase for butanol production
US9580705B2 (en) 2013-03-15 2017-02-28 Butamax Advanced Biofuels Llc DHAD variants and methods of screening

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101297042A (zh) * 2005-10-26 2008-10-29 纳幕尔杜邦公司 四碳醇的发酵生产

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