CN104521469A - Dendrobium soft rot controlling methods - Google Patents
Dendrobium soft rot controlling methods Download PDFInfo
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- CN104521469A CN104521469A CN201410722021.4A CN201410722021A CN104521469A CN 104521469 A CN104521469 A CN 104521469A CN 201410722021 A CN201410722021 A CN 201410722021A CN 104521469 A CN104521469 A CN 104521469A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- Agronomy & Crop Science (AREA)
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- Pest Control & Pesticides (AREA)
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Abstract
The invention relates to the technical field of traditional Chinese medicine material cultivation, and specifically relates to a dendrobium officinale artificial cultivation method. The invention discloses 2 endophytic fungus strains with biological controlling effects against orchidaceae dendrobium plant soft rot, and two soft rot controlling methods. The strains can colonize at dendrobium plant roots, and can be used in dendrobium plant seedling raising or cultivation. Soft rot can also be controlled with an artificial method. With the methods provided by the invention, dendrobium plant soft rot can be effectively controlled.
Description
Technical field
The present invention relates to technical field of Chinese herbal medicine cultivation, specifically a kind of prevention and controls of dendrobium candidum.
Background technology
Dendrobium candidum is China's tradition rare traditional Chinese medicine, occupies first of " Chinese nine immortal grass ".Before the 90's of 20th century, dendrobium candidum mainly relies on wild resource, and due to the exhaustion of wild resource, within 1987, State Council is classified as National Wild Conservative Plants.Last century Mo neighbouring province Zhejiang takes the lead in breaching the technology such as dendrobium candidum tissue cultivation, facility cultivation, and since entering the new century, large health industry implementation, the growth of " blowout formula " has appearred in cultivated area.
The extermination of disease and insect pest mainly adopts and relies mainly on prevention, method for integrated control, strictly isolates as carried out external application shading screen in foreign material around place pretreatment, place cleaning, canopy.The control of dendrobium candidum disease, ventilation is crucial.Prohibit the use high poison, persistent pesticide, use partial chemical agricultural chemicals limitedly.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, and the ground cultivating method that output is high, active constituent content is high is provided.
The present invention is achieved in that the control of soft rot: control irrigation amount well; With agricultural streptomycin 4000 times of liquid and tpn 1000 time serious.The mixing of times liquid or agricultural 4000 times of liquid and iprodione 1000 times of liquid sprayings, sprayed once every 7-10 days, continuous spraying 2-3 time, spraying time before at 10 in the morning with at 4 in afternoon after.
The present invention is improved further: the control of soft rot: forbid that plant matrix ponding or plant band water spend the night rainy season, reduce nitrogenous fertilizer usage amount, with high phosphorus potash fertilizer or impose the fertilizer such as 0.05% potassium sulfate and build up resistance, find that diseased plant is removed immediately together with its surrounding substrate, spray with agricultural streptomycin 600 times of liquid and iprodione 1000 times of liquid mixing time serious.
The present invention is improved further: a kind of fungi stem of noble dendrobium soft rot being had to preventive and therapeutic effect, it is characterized in that: their Classification And Nomenclature is respectively: the fungus Trichoderma Df188 of deposit number is the rhizopus fungi Dhs96 of CGMCC No.3786 to be Rhizopus sp. and deposit number be CGMCCNo.3787 is Trichoderma sp., for the seedling dish of Dendrobium Sw, flowerpot or seedbed nursery, the field production of Dendrobium Sw also can be used for.
Embodiment
Officinal dendrobium stem plantation of the present invention plants material, and it is embodiment preferably:
Embodiment 1
The present invention is achieved in that the control of soft rot: control irrigation amount well; With agricultural streptomycin 4000 times of liquid and tpn 1000 time serious.The mixing of times liquid or agricultural 4000 times of liquid and iprodione 1000 times of liquid sprayings, sprayed once every 7-10 days, continuous spraying 2-3 time, spraying time before at 10 in the morning with at 4 in afternoon after.
Embodiment 2
The present invention is improved further: the control of soft rot: forbid that plant matrix ponding or plant band water spend the night rainy season, reduce nitrogenous fertilizer usage amount, with high phosphorus potash fertilizer or impose the fertilizer such as 0.05% potassium sulfate and build up resistance, find that diseased plant is removed immediately together with its surrounding substrate, spray with agricultural streptomycin 600 times of liquid and iprodione 1000 times of liquid mixing time serious.
Embodiment 3
Endogenetic fungus Dhs96 mixes with wheat bran solid matrix and makes solid vaccination agent after PDA liquid fermentation and culture.Collect spore after stem of noble dendrobium soft rot nosophyte Pythium ultimum (Pythium ultimum) activated cultivation, be mixed with the spore suspension of 5 × 105/ml, mix with wheat bran solid matrix and make solid vaccination agent.Using bark as cultivation matrix, when planting HERBA DENDROBII seedling, the agent of Dhs96 solid vaccination is inoculated into the root of HERBA DENDROBII seedling.Potted orchard puts into greenhouse by solar heat, and temperature maintains 20 ± 2 DEG C.After plantation, water spray, is beneficial to the growth of endogenetic fungus.Every three days later water sprays once.Around HERBA DENDROBII seedling, the agent of pathogen solid vaccination is spread respectively at when 3 after inoculated fungi Dhs96 day and 30 days.Negative control is the HERBA DENDROBII seedling of the only access wheat bran solid matrix when planting; Positive control is the access wheat bran solid matrix when planting, and around HERBA DENDROBII seedling, spreads the agent of pathogen solid vaccination respectively at when 3 days after plantation and 30 days.The survival rate of seedling is added up after two months respectively at access pathogen.Two kinds of inoculation process, the average viability of seedling reaches 78.6%, and in negative control, the survival rate of seedling is 56.7%, does not have seedling to survive in positive control.This result shows: after infecting soft rot nosophyte, endogenetic fungus Dhs96 can improve the survival rate of HERBA DENDROBII seedling.
Embodiment 4
Endogenetic fungus Df188 makes the spore suspension of 1 × 107/ml after PDA liquid fermentation and culture, is mixed and made into solid vaccination agent with wheat bran solid matrix.Collect spore after stem of noble dendrobium soft rot nosophyte Pythium ultimum (Pythium ultimum) activated cultivation, be mixed with the spore suspension of 5 × 105/ml, mix with wheat bran solid matrix, make solid vaccination agent.Using bark as cultivation matrix, when planting HERBA DENDROBII seedling, the agent of Df188 solid vaccination is inoculated into the root of HERBA DENDROBII seedling.Potted orchard puts into greenhouse by solar heat, and temperature maintains 20 ± 2 DEG C.After plantation, water spray, is beneficial to the growth of endogenetic fungus.Every three days later water sprays once.Around HERBA DENDROBII seedling, the agent of pathogen solid vaccination is spread respectively at when 3 after inoculated fungi Df188 day and 30 days.Negative control is the HERBA DENDROBII seedling of the only access wheat bran solid matrix when planting; Positive control is the access wheat bran solid matrix when planting, and around HERBA DENDROBII seedling, spreads the agent of pathogen solid vaccination respectively at when 3 days after transplanting and 30 days.The survival rate of seedling is added up after two months respectively at access pathogen.Two kinds of inoculation process, the average viability of seedling reaches 73.3%, and in negative control, the survival rate of seedling is 56.7%, does not have seedling to survive in positive control.This result shows: after infecting soft rot nosophyte, endogenetic fungus Df188 can improve the survival rate of HERBA DENDROBII seedling.
Claims (3)
1. a Disease-Control for Soft-Rotting method for the stem of noble dendrobium, is characterized in that: the steps include:
The control of soft rot: control irrigation amount well; With agricultural streptomycin 4000 times of liquid and tpn 1000 time serious
The mixing of times liquid or agricultural 4000 times of liquid and iprodione 1000 times of liquid sprayings, sprayed once every 7-10 days, continuous spraying 2-3 time, spraying time is before at 10 in the morning or after at 4 in afternoon.
2. the Disease-Control for Soft-Rotting method of a kind of stem of noble dendrobium according to claim 1, is characterized in that: the steps include:
The control of soft rot: forbid that plant matrix ponding or plant band water spend the night rainy season, reduce nitrogenous fertilizer usage amount, with high phosphorus potash fertilizer or impose the fertilizer such as 0.05% potassium sulfate and build up resistance, find that diseased plant is removed immediately together with its surrounding substrate, spray with agricultural streptomycin 600 times of liquid and iprodione 1000 times of liquid mixing time serious.
3. one kind has the fungi of preventive and therapeutic effect to stem of noble dendrobium soft rot, it is characterized in that: their Classification And Nomenclature is respectively: deposit number is the rhizopus fungi Dhs96 of CGMCC No.3786, described fungi is Rhizopus sp. and deposit number is the fungus Trichoderma Df188 of CGMCCNo.3787, described fungi is Trichoderma sp., for the seedling dish of Dendrobium Sw, flowerpot or seedbed nursery, the field production of Dendrobium Sw also can be used for.
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| CN201410722021.4A CN104521469A (en) | 2014-12-03 | 2014-12-03 | Dendrobium soft rot controlling methods |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104844361A (en) * | 2015-05-12 | 2015-08-19 | 柳州市耕青科技有限公司 | Protective nutrient solution special for soft rot of dendrobium officinale |
Citations (6)
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| CN102234618A (en) * | 2010-05-05 | 2011-11-09 | 中国医学科学院药用植物研究所 | Two endophytic fungi strains having control effect on soft rot disease of dendrobium plants |
| CN102776127A (en) * | 2010-05-05 | 2012-11-14 | 中国医学科学院药用植物研究所 | Endophytic fungus with preventive and treatment effects on soft rot of dendrobiums |
| CN102845212A (en) * | 2012-10-04 | 2013-01-02 | 向华 | High-efficiency interplanting method for momordica grosvenori and dendrobium officinale |
| CN103609416A (en) * | 2013-11-30 | 2014-03-05 | 桂平市白石洞天仙山铁皮石斛专业合作社 | Planting method for dendrobium officinale |
| CN104025987A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale planting method |
| CN104094918A (en) * | 2014-07-10 | 2014-10-15 | 无锡双喜金阳生物科技有限公司 | Pest control method for ecologically cultivating dendrobium officinale |
-
2014
- 2014-12-03 CN CN201410722021.4A patent/CN104521469A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234618A (en) * | 2010-05-05 | 2011-11-09 | 中国医学科学院药用植物研究所 | Two endophytic fungi strains having control effect on soft rot disease of dendrobium plants |
| CN102776127A (en) * | 2010-05-05 | 2012-11-14 | 中国医学科学院药用植物研究所 | Endophytic fungus with preventive and treatment effects on soft rot of dendrobiums |
| CN102845212A (en) * | 2012-10-04 | 2013-01-02 | 向华 | High-efficiency interplanting method for momordica grosvenori and dendrobium officinale |
| CN103609416A (en) * | 2013-11-30 | 2014-03-05 | 桂平市白石洞天仙山铁皮石斛专业合作社 | Planting method for dendrobium officinale |
| CN104025987A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale planting method |
| CN104094918A (en) * | 2014-07-10 | 2014-10-15 | 无锡双喜金阳生物科技有限公司 | Pest control method for ecologically cultivating dendrobium officinale |
Non-Patent Citations (2)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104844361A (en) * | 2015-05-12 | 2015-08-19 | 柳州市耕青科技有限公司 | Protective nutrient solution special for soft rot of dendrobium officinale |
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