CN103858645A - Greenhouse planting method of anoectochilus roxburghii lindl tissue culturing seedlings - Google Patents
Greenhouse planting method of anoectochilus roxburghii lindl tissue culturing seedlings Download PDFInfo
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- CN103858645A CN103858645A CN201410106481.4A CN201410106481A CN103858645A CN 103858645 A CN103858645 A CN 103858645A CN 201410106481 A CN201410106481 A CN 201410106481A CN 103858645 A CN103858645 A CN 103858645A
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Abstract
The invention discloses a greenhouse planting method of anoectochilus roxburghii lindl tissue culturing seedlings. The greenhouse planting method comprises the following steps of matrix disinfecting: slightly-acidic matrixes which are formed by mixing peat soils, pine needle soils and river sands in a 8:1:1 volume proportion are adopted by planting matrixes, and a steam sterilizing method is adopted for carrying out disinfecting treatment before planting; planting: the anoectochilus roxburghii lindl tissue culturing seedlings are planted on the disinfected matrixes, single-plant deep planting is carried out, and the depth is subject to a situation that first stem nodes above roots are embedded in the matrixes, and second stem nodes get close to the surface layers of the matrixes; environment controlling: the planted seedlings are put in a greenhouse of which the light illumination, the temperature and the humidity can be controlled for being cultured, the light illumination is controlled under 3000 Lux, the temperature is controlled under 30 DEG C, and the humidity is maintained to be greater than 80%. According to the greenhouse planting method of the anoectochilus roxburghii lindl tissue culturing seedlings, which is disclosed by the invention, no pesticide is needed to be used by the anoectochilus roxburghii lindl planting, no pest and disease damage is generated, the plants are robust, and the survival rate is high.
Description
Technical field
The present invention relates to biological technical field, refer in particular to the greenhouse gardening method of a kind of roxburgh anoectochilus terminal bud tissue culturing seedling.
Background technology
Roxburgh anoectochilus terminal bud (Anoectochilus roxburghii (Wall.) Lindl) is the herbaceos perennial that the orchid family is opened lip Cymbidium, another name Shorthairy Antenoron, rough melic herb, gold thread taiwan anetochilus herb, black ginseng etc., be a kind of traditional valuable ingredient of traditional Chinese medicine, there is clearing heat and detoxicating, expelling wind and removing dampness, the effect such as nourishing Yin and moistening lung, hypotensive.Meanwhile, roxburgh anoectochilus terminal bud plant type is small and exquisite attractive in appearance, leaf grace, and vein golden yellow, is netted arrangement, has high ornamental value.
Roxburgh anoectochilus terminal bud seed nature germination rate and reproduction rate are low, and poor growth, adds artificially and excavate in a large number, the destruction of ecotope, and wild roxburgh anoectochilus terminal bud resource is day by day exhausted.Cultivate by tissue the vegetative propagation system of setting up, the protection of roxburgh anoectochilus terminal bud resource and rationally utilization are created conditions.
In prior art, artificial planting roxburgh anoectochilus terminal bud tissue-culture container seedling hardening is very general, but because roxburgh anoectochilus terminal bud requires harshness to planting environment, easy infection damage by disease and insect, survival rate is not high, and all large area is used agricultural chemicals.
Summary of the invention
The object of the present invention is to provide the greenhouse gardening method of a kind of roxburgh anoectochilus terminal bud tissue culturing seedling, make roxburgh anoectochilus terminal bud plantation without using agricultural chemicals, and occur without damage by disease and insect, robust plant, survival rate is high.
For reaching above-mentioned purpose, solution of the present invention is:
A greenhouse gardening method for roxburgh anoectochilus terminal bud tissue culturing seedling, comprises the following steps:
Matrix sterilization: the slant acidity matrix that planting matrix adopts peat soil, pine soil, river sand 8:1:1 volume ratio to mix, adopts steam sterilization to disinfect before plantation;
Plantation: roxburgh anoectochilus terminal bud group training seed is implanted in the matrix through disinfecting, and individual plant is planted deeply, the degree of depth is imbedded in matrix with above the first stipes of root, and the second stipes approaches matrix top layer and is as the criterion;
Environment control: kind is planted seedlings and is placed on interior cultivation of booth of controllable light photograph, temperature, humidity, and below illumination control 3000Lux, temperature is controlled at below 30 ℃, and humidity remains on more than 80%.
Also comprise hardening step before described matrix sterilisation step: tissue-culture container seedling is placed on controllable light according to hardening in the booth of, temperature 20 days, wherein last 5 days is the hardening of uncapping, condition of culture is below intensity of illumination 3500Lux, day light application time 10 h, and temperature is below 30 degrees Celsius.
Described matrix steam sterilization conditions for sterilization is: passing into after steam, sterilization pool matrix central area temperature reaches 75 ℃, and is incubated 1 hour.
When described environment control, can within 1-3 days, water normal root water according to matrix humidity, the later stage is carried out atomized water pouring, can carry out amino acids nutriment and spray foliage fertilizer while cultivating one month.
Adopt after such scheme, greenhouse gardening method of the present invention has solved roxburgh anoectochilus terminal bud group tissue culturing seedling and has adopted conventional method easy infection damage by disease and insect, survival rate is low, large area is used the problems such as agricultural chemicals, realize industrialization and planted fast, planted after 5 months and plant the stalwartness of planting seedlings, occurred without damage by disease and insect, individual plant counterpoise is greater than 1.89g, and survival rate is more than 98%.
Embodiment
Embodiment
The present invention discloses the greenhouse gardening method of a kind of roxburgh anoectochilus terminal bud tissue culturing seedling, comprises the following steps
One, tissue-culture container seedling hardening
Roxburgh anoectochilus terminal bud tissue-culture container seedling moves to hardening canopy from culturing room, in the booth of controllable light photograph, temperature, carries out hardening 20 days, within first 15 days, is covered experienced seedling, and last 5 days is the hardening of uncapping.The object of the hardening degree of lignification that makes to grow seedlings improves, fully root of hair, and stem growth is sturdy, and the object of uncapping makes to grow seedlings and adapts to better planting environment.The illumination of hardening canopy is controlled at below 3500Lux, every day light application time 10 h, temperature is below 30 ℃.
Two, matrix sterilization
Matrix all by volume ratio mix, put into matrix sterilization pool, cover with plastic foil.Insert stationary point thermometer in sterilization pool matrix central area.Pass into steam, sterilization pool matrix central area temperature starts timing while reaching 75 ℃, be incubated 1 hour.Adopt steam sterilization to carry out matrix and disinfect, can kill weed seed, worm's ovum and most of virus.Pine soil is pulverized fermentation 90 days by the pine soil gathering.
Three, plantation
Tissue-culture container seedling takes out in bottle, with clear water, bottle shoot root portion medium is rinsed well and is tiled to dry and wait to plant, and roxburgh anoectochilus terminal bud group training seedling average height is 5.2cm, and average weight is 0.9g.Adopt individual plant deeply to plant mode, plant and be contained on the dish of cave through the matrix of disinfecting.The degree of depth is imbedded in matrix with above the first stipes of root, and the second stipes approaches topsoil and is as the criterion.This method is planted generation and the growth of favourable aerial root, makes plant from malaria, absorb moisture.
Four, environment control
Kind is planted seedlings and is placed on interior cultivation of booth of controllable light photograph, temperature, humidity, and below illumination control 3000Lux, temperature is below 30 ℃, and humidity remains on more than 80%.After bottle seed is good, water blade face water, can within 1-3 days, water normal root water according to matrix humidity, the later stage is carried out atomized water pouring, cultivates after one month, can within every 15 days, carry out amino acids nutriment and spray blade face.
The impact on plantation of different substrates and different proportioning is below provided
Experiment is carried out in two steps, and roxburgh anoectochilus terminal bud group training seedling average height is 5.2cm, and average weight is 0.9g, adopts individual plant deeply to plant mode, plants and is being contained on the dish of cave through the matrix of disinfecting, and the degree of depth is imbedded in matrix with the first stipes, and the second stipes approaches topsoil and is as the criterion.
Plant and after 5 months, see the following form 1 and table 2, the seedling stalwartness that adopts peat soil, pine soil, river sand 8:1:1 kind to plant, occurs without damage by disease and insect, individual plant counterpoise >1.89g, and survival rate is more than 98%.The impact of table 1 different substrates on roxburgh anoectochilus terminal bud plantation
The impact of table 2 substrate composition on roxburgh anoectochilus terminal bud plantation
Claims (4)
1. a greenhouse gardening method for roxburgh anoectochilus terminal bud tissue culturing seedling, is characterized in that, comprises the following steps:
Matrix sterilization: the slant acidity matrix that planting matrix adopts peat soil, pine soil, river sand 8:1:1 volume ratio to mix, adopts steam sterilization to disinfect before plantation;
Plantation: roxburgh anoectochilus terminal bud group training seed is implanted in the matrix through disinfecting, and individual plant is planted deeply, the degree of depth is imbedded in matrix with above the first stipes of root, and the second stipes approaches matrix top layer and is as the criterion;
Environment control: kind is planted seedlings and is placed on interior cultivation of booth of controllable light photograph, temperature, humidity, and below illumination control 3000Lux, temperature is controlled at below 30 ℃, and humidity remains on more than 80%.
2. the greenhouse gardening method of a kind of roxburgh anoectochilus terminal bud as claimed in claim 1 tissue culturing seedling, it is characterized in that, also comprise hardening step before described matrix sterilisation step: tissue-culture container seedling is placed on controllable light according to hardening in the booth of, temperature 20 days, wherein last 5 days is the hardening of uncapping, condition of culture is below intensity of illumination 3500Lux, day light application time 10 h, temperature is below 30 degrees Celsius.
3. the greenhouse gardening method of a kind of roxburgh anoectochilus terminal bud as claimed in claim 1 tissue culturing seedling, is characterized in that, described matrix steam sterilization conditions for sterilization is: passing into after steam, sterilization pool matrix central area temperature reaches 75 ℃, and is incubated 1 hour.
4. the greenhouse gardening method of a kind of roxburgh anoectochilus terminal bud as claimed in claim 1 tissue culturing seedling, it is characterized in that, when described environment control, can within 1-3 days, water normal root water according to matrix humidity, later stage is carried out atomized water pouring, can carry out amino acids nutriment and spray foliage fertilizer while cultivating one month.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686309A (en) * | 2015-02-26 | 2015-06-10 | 浙江农林大学 | Seedling hardening and transplant acclimatization method for roxburgh anoectochilus terminal bud tissue culture seedlings |
| CN105075605A (en) * | 2015-08-17 | 2015-11-25 | 厦门锦泰谷农业科技有限公司 | Planting method for increasing survival rate of anoectochilus roxburghii seedlings |
| CN105766262A (en) * | 2014-12-23 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Greenhouse planting method for Anoectochilus roxburghii |
| CN106171470A (en) * | 2016-08-03 | 2016-12-07 | 宁明县红枫中药材种植专业合作社 | The greenhouse gardening method of Herba Anoectochili roxburghii |
| CN106857138A (en) * | 2017-01-09 | 2017-06-20 | 厦门涌泉科技有限公司 | A kind of implantation methods for rolling into a ball flower tissue-cultured seedling |
| CN107211696A (en) * | 2017-06-21 | 2017-09-29 | 柳城新天地生态农业发展有限公司 | The implantation methods of roxburgh anoectochilus terminal bud |
| CN107333558A (en) * | 2017-08-30 | 2017-11-10 | 广东茂丰源农业科技有限公司 | A kind of packed roxburgh anoectochilus terminal bud implantation methods of sealing |
| CN110352804A (en) * | 2019-08-15 | 2019-10-22 | 江西环境工程职业学院 | A kind of acclimatization and transplants method of savatier monochasma herb |
| CN112618658A (en) * | 2020-12-29 | 2021-04-09 | 联和东浩(厦门)药业有限公司 | Processing method of traditional Chinese medicine decoction pieces containing anoectochilus formosanus |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766262A (en) * | 2014-12-23 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Greenhouse planting method for Anoectochilus roxburghii |
| CN104686309A (en) * | 2015-02-26 | 2015-06-10 | 浙江农林大学 | Seedling hardening and transplant acclimatization method for roxburgh anoectochilus terminal bud tissue culture seedlings |
| CN105075605A (en) * | 2015-08-17 | 2015-11-25 | 厦门锦泰谷农业科技有限公司 | Planting method for increasing survival rate of anoectochilus roxburghii seedlings |
| CN106171470A (en) * | 2016-08-03 | 2016-12-07 | 宁明县红枫中药材种植专业合作社 | The greenhouse gardening method of Herba Anoectochili roxburghii |
| CN106857138A (en) * | 2017-01-09 | 2017-06-20 | 厦门涌泉科技有限公司 | A kind of implantation methods for rolling into a ball flower tissue-cultured seedling |
| CN107211696A (en) * | 2017-06-21 | 2017-09-29 | 柳城新天地生态农业发展有限公司 | The implantation methods of roxburgh anoectochilus terminal bud |
| CN107333558A (en) * | 2017-08-30 | 2017-11-10 | 广东茂丰源农业科技有限公司 | A kind of packed roxburgh anoectochilus terminal bud implantation methods of sealing |
| CN110352804A (en) * | 2019-08-15 | 2019-10-22 | 江西环境工程职业学院 | A kind of acclimatization and transplants method of savatier monochasma herb |
| CN112618658A (en) * | 2020-12-29 | 2021-04-09 | 联和东浩(厦门)药业有限公司 | Processing method of traditional Chinese medicine decoction pieces containing anoectochilus formosanus |
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| CN103858645B (en) | 2015-09-02 |
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Effective date of registration: 20151230 Address after: 361000, Fujian, Xiamen province Haicang District East Fu town Yongquan tree seed base office building, room 103 Patentee after: XIAMEN LIAN LE BIOTECHNOLOGY CO., LTD. Address before: 361000, Fujian, Xiamen province Jimei Irrigation Town fish floating Industrial Zone Patentee before: Yongquan Science & Technology Co., Ltd., Xiamen |
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Effective date of registration: 20191129 Address after: 361000 room 105, No. 666-1, Dongfu Avenue, Dongfu street, Haicang District, Xiamen City, Fujian Province Patentee after: Xiamen lelianle Biotechnology Co., Ltd Address before: 361000, Fujian, Xiamen province Haicang District East Fu town Yongquan tree seed base office building, room 103 Patentee before: XIAMEN LIANLE BIOTECHNOLOGY CO., LTD. |