CN101642036A - Application process of fungus biocontrol agent - Google Patents
Application process of fungus biocontrol agent Download PDFInfo
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Abstract
The invention discloses an application process of fungus biocontrol agents, belonging to the application of microorganism. The existing fungus biocontrol product has the problems that the applicationprocess needs a large amount of labor employment, the spore germination is poor and the colonization is unsatisfactory. The application process of fungus biocontrol agents is to spread the fungus biocontrol agents containing biocontrol fungal spore agent and nutrient medium on wet soil surface so as to enable the biocontrol fungus to grow and propagate fast. The method of the invention comprises the following steps: irrigating, spraying water, sprinkling or performing drip irrigation or filtration irrigation in advance to wet the soil and then spreading the fungus biocontrol agents on wet soilsurface; the method is simple and practicable, can save labor and is easy to accept for users. By using the method to employ the fungus biocontrol agents, the nutrient medium can provide nutrition for the biocontrol fungus on soil surface so as to ensure the biocontrol fungus to grow and propagate fast, which can not only prevent the fungal diseases on the root and the stem base of plants, but also prevent the fungal diseases of the stems, leaves, flowers and fruits of plants.
Description
The invention belongs to the microorganism application, be specifically related to the application process of fungus biocontrol agent.
Now, fungal diseases of plants has restricted high yield, stable yields and the high-quality of crop.The raising day by day that pollution-free, nuisanceless green organic food is required along with people, expert, scholar are devoted to not have the residual biological control research of chemical pesticide and do one's utmost, and filter out that mould as wood, sticking broom is mould, the shield shell is mould, chactomium globosum, Paecilomyces lilacinus etc. some have antagonism disease fungi mycota fungi function, that can be used for biological control and (be called for short: biocontrol fungi).What develop in recent years, register both at home and abroad is that the fungus biocontrol agent quantity of biological control bacterial classification also has certain scale with the biocontrol fungi, but fails so far to be widely used, and tracing it to its cause is that fungus biocontrol agent haves much room for improvement on application process.
Existing application process mainly is to mix soil to spread fertilizer over the fields, directly admix plow layer, plant plant hole, irritate root or spray.Such application process not only recruitment is big, and because of being subjected to the influence of the antibacterial factor and residue of pesticide in the soil, spore germination, the mycelial growth of biocontrol fungi are poor, grow undesirable surely.Spray in this way, limit to because of being subjected to weather, environmental influence and spraying technology own, the biocontrol fungi spore will be sprouted, grow surely more difficult at stem, leaf, flower, the fruit position of crop.Because the spore of biocontrol fungi becomes current shorter, in the transportation of commodity preparation with in storing the part inactivation is arranged, under the situation that the spore amount of living reduces, above-mentioned application process influences sprouting, the growth of biocontrol fungi especially and grows surely.
The technical assignment of the technical problem to be solved in the present invention and proposition is to improve application process, what overcome fungus biocontrol agent product that prior art exists uses that recruitment is big, the sprouting of spore is poor, grow, grow shortcomings such as undesirable surely, a kind of application process of fungus biocontrol agent is provided.For this reason, the present invention is by the following technical solutions:
The application process of fungus biocontrol agent is characterized in that the fungus biocontrol agent that will comprise agent of biocontrol fungi spore and nutrition base spreads fertilizer over the fields on damp soil (or for various materials plant material) surface, makes biocontrol fungi spore fast-germination, raised growth, expansion numerous.
The invention has the beneficial effects as follows:
One, the former method of using fungus biocontrol agent are to mix native hundreds of times to spread fertilizer over the fields or admix plow layer, plant plant hole with the centesimal ratio of number, and such application process recruitment is big, and practical application is had any problem.
Of the present inventionly use the fungus biocontrol agent method as long as spread fertilizer over the fields to damp soil (or for various materials plant material) surface, method is simple, and recruitment province allows the user accept easily.
Two, former amount of application is excessive, and by plow layer, plant plant hole and mix and excute a law, every mu of the consumption of solid powder is more than double centner, and cost is higher.
Use the present invention, every mu of consumption only need be with 1.5 kilograms to 10 kilograms, and cost alleviates tens of times.
Three, effect aspect, fungus biocontrol agent is mixed soil and is spread fertilizer over the fields, admixes plow layer, plants plant hole, method such as irritate root or spray, be subjected to the antibacterial factor in the soil influence, spray technology limitation own, spore germination is poor, be difficult to reach general effect of comprehensively growing surely, adding commodity preparation fungal spore in the storage transportation has the part inactivation, influences protection effect especially.
Implementing advantage of the present invention is, by the field proliferating effect, promptly work as the fungi biocontrol agent and be sprinkling upon damp soil (or being the material of planting of various materials) surface, the very fast moisture absorption of fungus biocontrol agent, under suitable temperature, biocontrol fungi spore germination, mycelial growth in 6 hours just can grow into bacterium colony in 12 hours, and bacterium colony can produce spore in 24 hours.Fungus biocontrol agent spread fertilizer over the fields in one week of back, and every gram can produce spore more than 1,000,000,000.These fresh and alive spores are with flow air between plant, are diffused into each corner of soil surface and axis, leaf, flower, really, sprout in suitable place, growth and growing surely.
In the said process, the biocontrol fungi mycelia of fungus colony bottom can grow in soil fast because of obtaining the abundant nutrition of nutrition base, grows in soil surely.
The fungus biocontrol agent dispensing can obtain sufficient oxygen, and not be subjected to the influence of the antibacterial factor in the soil on soil (or being the material of planting of various materials) surface, and growth rate is than fast several times in soil, and this is of the present invention one big characteristics.
Implementing the present invention prevents the mechanism of plant root, basal part of stem fungal disease to be, the fungus biocontrol agent dispensing is grown fast, is bred and grow surely in soil at soil (or being the material of planting of various materials) back, surface biocontrol fungi, suppress the sprouting of disease fungal spore in the soil (or being the material of planting of various materials) and the growth of disease hypha,hyphae, inhibition disease fungi is infected plant, reaches the purpose that the prevention fungal diseases of plants takes place.
Implement the present invention and prevent axis, leaf, flower if really the mechanism of fungus diseases is, biocontrol fungi is grown fast, is bred on soil (or being the material of planting of various materials) surface, at first suppress to endanger axis, leaf, flower, the sprouting of disease fungal spore really and the growth of mycelia in the soil (or being the material of planting of various materials), reduce the dynamics that infects of disease fungi.Biocontrol fungi produces a large amount of spores and discharges, disperses in the field in the surperficial reproductive process of soil (or being the material of planting of various materials), the part spore can land may be played a protective role by the position that the disease fungi encroaches in axis, leaf, flower, fruit; Biocontrol fungi can discharge a large amount of volatility antibiotic in growth, reproductive process, can suppress in the soil (or being the material of planting of various materials) and axis, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.This is another big characteristics of the present invention.
The effect of implementing the inventive method prevention plant roots, basal part of stem fungal disease is sustainable more than two months; Prevention axis, leaf, flower, fruit position fungal disease effect are sustainable more than 25 days.
Below specify the application process of fungus biocontrol agent of the present invention.
The application process of fungus biocontrol agent, be that the fungus biocontrol agent that will comprise agent of biocontrol fungi spore and nutrition base spreads fertilizer over the fields on damp soil (or being the material of planting of various materials) surface, superficial layer is quick, raised growth, produces spore at soil (or being the material of planting of various materials) to make biocontrol fungi.
Concrete may further comprise the steps: (1) to the soil of land for growing field crops, booth, greenhouse, cultivated plant such as the potted plant material of planting of various materials (or for) pour water in advance, spray water, watering or drip irrigation, infiltrating irrigation, make soil (or being the material of planting of various materials) moistening; Descend as the moistening situation of soil behind rain (or for various materials plant material), promptly needn't pour water again, spray water, watering or drip irrigation, infiltrating irrigation.
(2) fungus biocontrol agent is evenly spread fertilizer over the fields on damp soil (or being the material of planting of various materials) surface.
At the different stage of crop, should distinguish to some extent and spread fertilizer over the fields:
With after planting and before the seedling sowing, fungus biocontrol agent is evenly spread fertilizer over the fields on the soil material of planting of various materials (or for) surface before the A, planting seed;
B, seedling after planting and the plant growing stage evenly spread fertilizer over the fields soil between the plant material of planting of various materials (or for) surface to fungus biocontrol agent, avoid being sprinkling upon the blade face and directly are sprinkling upon the foundation place;
C, to potted plant, fungus biocontrol agent is evenly spread fertilizer over the fields surface at basin limit soil (or for various materials plant material).
Fungus biocontrol agent is sprinkling upon the blade face can influence its effect, is sprinkling upon the foundation place crop of can burning in a large number, therefore will avoid spreading fertilizer over the fields like this.
Spreading fertilizer over the fields fungus biocontrol agent should be afternoon to carrying out at dusk.To before the planting seed with after planting and before the seedling sowing, the less field of plant spreads fertilizer over the fields fungus biocontrol agent and should carry out behind not direct projection of sun soil face.
The amount of application of fungus biocontrol agent is that per hectare is with 22.5 kilograms～150 kilograms.
Fungus biocontrol agent of the present invention is the homogeneous mixture of agent of biological and ecological methods to prevent plant disease, pests, and erosion fungal spore and nutrition base.The agent of described biocontrol fungi spore is for cultivating the solid or liquid or the solid and the mixtures of liquids that contain a large amount of biocontrol fungi spores and a small amount of biocontrol fungi viable bacteria filament of gained to biocontrol fungi.Described nutrition base provides the nutriment of carbon, nitrogen and mineral matter and trace element for growing, produce spore for biocontrol fungi fast.Before mixing with the agent of biocontrol fungi spore, the nutrition base under 120 ± 5 ℃ of conditions, sterilized 30 ± 5 minutes.
The advantage of the application process of fungus biocontrol agent:
The application process of one, former fungus biocontrol product is to mix that hundreds of times in soil spreads fertilizer over the fields or admix plow layer, plant plant hole with the centesimal ratios of number, and such application process recruitment is big, and practical application is had any problem.
Implement the application process of this fungi biocontrol agent as long as spread fertilizer over the fields to moistening plow layer surface, method is simple, allows the user accept easily.
Two, former amount of application is excessive, and by plow layer, plant plant hole and mix and excute a law, every mu of the consumption of solid biological and ecological methods to prevent plant disease, pests, and erosion pulvis is more than double centner, and cost is higher.
The application process of implementing this fungi biocontrol agent only needs with 1.5 kilograms～10.0 kilograms for every mu, and cost alleviates tens of times.
It three is effect aspects, the biocontrol fungi conidial powder is mixed soil and is spread fertilizer over the fields, admixes plow layer, plants plant hole, method such as irritate root or spray, be subjected to the antibacterial factor in the soil influence, spray technology limitation own, the biocontrol fungi spore germination is poor, be difficult to reach the effect that biocontrol fungi is generally grown comprehensively surely, add commodity preparation part fungal spore inactivation in the storage transportation, influence protection effect.
The advantage of implementing the application process of this fungi biocontrol agent is: when the fungi biocontrol agent is sprinkling upon the damp soil surface, the very fast moisture absorption of fungus biocontrol agent, under suitable temperature, biocontrol fungi spore germination, mycelial growth in 6 hours, just can grow into bacterium colony in 12 hours, bacterium colony can produce spore in 24 hours.Fungus biocontrol agent spread fertilizer over the fields behind the field in the week, and every gram can produce spore more than 1,000,000,000.These fresh and alive spores are with flow air between crop, are diffused into each corner of soil surface and stem of plant, leaf, flower, really, sprout growth and growing surely in suitable place.
In the said process, the biocontrol fungi mycelia of bacterium colony bottom can grow in soil fast because of obtaining the abundant nutrition of nutrition base, grows in soil surely.
Implement this method and spread fertilizer over the fields fungus biocontrol agent in soil surface, biocontrol fungi can obtain sufficient oxygen, and is not subjected to the influence of the antibacterial factor in the soil, and growth rate is than fast several times in soil, and this is of the present invention one big characteristics.
The mechanism of implementing this method prevention plant root, basal part of stem fungal disease is, fungus biocontrol agent dispensing biocontrol fungi behind the damp soil surface is grown fast, is bred and grows surely in soil, suppress the sprouting of disease fungal spore in the soil and the growth of disease hypha,hyphae, thereby inhibition disease fungi is infected plant, reaches the purpose that the prevention fungal diseases of plants takes place.
Implement this method prevention axis, leaf, flower if really the mechanism of fungus diseases is, biocontrol fungi is grown at soil surface, breeding at first suppresses soil surface and may endanger axis, leaf, flower, the sprouting of disease fungal spore really and the growth of mycelia, reduces the dynamics that infects of disease fungi.Biocontrol fungi produces a large amount of spores and discharges, disperses in the field in soil surface growth, reproductive process, the part spore can land may be played a protective role by the position that the disease fungi encroaches in axis stem of plant, leaf, flower, fruit; Biocontrol fungi can discharge a large amount of volatility antibiotic in growth, reproductive process, can suppress soil surface and axis, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
Implement this method, use simple, cost is low, protection effect is obvious, and non agricultural chemical residuum is specially adapted to the pollution-less agriculture field.
Implement the diseases prevention example of the application process of this fungi biocontrol agent:
The prevention of example one, vegetables, grey mould fruit rot of strawberry
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum two strain bacterial strains (numbering T25 and T96 certainly).
The prescription of conidial powder medium: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 5mm.
The weight proportion of conidial powder and nutrition base is 25: 75, is 120 kilograms of per hectares at the morbidity season crop premorbid amount of application first time, and executing the applied once amount again in 1 month at interval is 45 kilograms of per hectares, and this example is executed secondary altogether, and protection effect reaches 91%.
The prevention of example two, cotton seedling blight (claiming the cotton seedling blight again)
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum one a strain bacterial classification (numbering T96 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, rice meal 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 30: 70, and per hectare is executed 45 kilograms before seedling is sowed, and executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 93%.
The prevention of example three, peanut damping off
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum one strain bacterial classification (numbering T96 certainly) and green fungi one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 35: 65, uses before sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 95%.
The prevention of example four, pimento and Phytophthora capsici disease
The bacterial classification that this example uses is fungus Trichoderma Trichoderma viride one a strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 40: 60, uses after sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 90%.
The prevention of example five, jasmine southern blight
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and green fungi one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder, spore liquid and nutrition base is 25: 20: 55, and 45 kilograms of per hectare consumptions are used at morbidity season crop premorbid, executes once in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 94%.
The prevention of example six, orchid fusarium wilt (claiming stem rot again)
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and green fungi one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 3.0mm.
The prevention of example seven, sclerotinia rot of colza
The bacterial classification that this example uses is Gliocladium fungi one a strain bacterial strain (numbering G21 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, rice meal 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 35: 65,, use according to the inventive method at morbidity season crop premorbid, the per hectare consumption is 60 kilograms for the first time, executes once 30 kilograms of per hectare consumptions in 1 month at interval later on, this example is executed 3 times altogether, and protection effect reaches 89%.
The prevention of example eight, eggplant damping off and southern blight
The bacterial classification that this example uses is Coniothyrium fungi one a strain bacterial strain (numbering C36 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn stalk powder 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, corn stalk powder 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 30: 70, use according to the inventive method at morbidity season crop premorbid, for the first time the per hectare consumption is 60 kilograms, executes 30 kilograms of per hectare consumptions later at interval in 1 month, this example is executed 3 times altogether, and protection effect reaches 92%.
The prevention of example nine, tomato wilt
The bacterial classification that this example uses is Chaetomium fungi one a strain bacterial strain (numbering C16 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 3.0mm.
The weight proportion of conidial powder and nutrition base is 35: 65, use according to the inventive method at morbidity season crop premorbid, for the first time the per hectare consumption is 60 kilograms, executes 30 kilograms of per hectare consumptions later at interval in 2 months, this example is executed 2 times altogether, and protection effect reaches 85%.
Remarks:
Expand numerous, i.e. expanding propagation; Field proliferating is promptly carried out expanding propagation in the field.
The expansion of biocontrol fungi in the past is numerous all to be finished in factory or laboratory, and the biocontrol fungi spore preparation that people will expand after numerous is directly discharged the field, and to reach the purpose of preventing and treating crop pest, its application process mainly contains two kinds:
One is the biocontrol fungi spore preparation is mixed that soil spreads fertilizer over the fields or directly to admix soil or admix kind of a plant hole, makes the biocontrol fungi spore sprout in soil, grow and grow in soil surely.
Its two, be with the biocontrol fungi spore preparation with dusting or converting water sprays, the biocontrol fungi spore is sprayed directly on to stem, leaf, flower, the fruit position of plant, make that it is sprouted at these positions, growth and growing surely.
Biocontrol epiphyte field propagation agent then is to form with agent of biocontrol fungi spore and nutrition base, and in the field, biocontrol fungi is grown, bred in the field with the biocontrol epiphyte field propagation agent dispensing, enlarges the spore amount.The biocontrol fungi spore that these expanding propagation are come out can be diffused in the soil that does not spread biocontrol epiphyte field propagation agent growth and grow surely, reaches the purpose of comprehensive protection.The biocontrol fungi spore that expanding propagation is come out can discharge, disperse, and the part spore can land may be sprouted, grow, grow surely in stem of plant, leaf, flower, fruit by the position that the disease fungi encroaches on, and plays a protective role.Biocontrol fungi discharges a large amount of volatility antibiotic in field growing, reproductive process, can suppress soil surface and stem of plant, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
Claims (6)
1, the application process of fungus biocontrol agent is characterized in that the fungus biocontrol agent that will comprise agent of biocontrol fungi spore and nutrition base spreads fertilizer over the fields on damp soil (or for various materials plant material) surface, makes biocontrol fungi fast-germination, growth, expansion numerous
2, the application process of fungus biocontrol agent according to claim 1, its feature may further comprise the steps:
(1) to the soil of land for growing field crops, booth, greenhouse, cultivated plant such as the potted plant material of planting of various materials (or for) pour water in advance, spray water, watering or drip irrigation, infiltrating irrigation, make soil (or being the material of planting of various materials) moistening; Descend as the moistening situation of soil behind rain (or for various materials plant material), promptly needn't pour water again, spray water, watering or drip irrigation, infiltrating irrigation.
(2) fungus biocontrol agent is evenly spread fertilizer over the fields on damp soil (or being the material of planting of various materials) surface.
3, the application process of fungus biocontrol agent according to claim 1 and 2 is characterized in that:
With after planting and before the seedling sowing, fungus biocontrol agent is evenly spread fertilizer over the fields on the soil material of planting of various materials (or for) surface before the A, planting seed;
B, seedling after planting and the plant growing stage evenly spread fertilizer over the fields soil between the plant material of planting of various materials (or for) surface to fungus biocontrol agent, avoid being sprinkling upon the blade face and directly are sprinkling upon the foundation place;
C, to the using of pot plant, should evenly spread fertilizer over the fields surface to fungus biocontrol agent at the basin limit soil material of planting of various materials (or for).
4, fungus biocontrol agent according to claim 1 is characterized in that described fungus biocontrol agent is the homogeneous mixture of agent of biological and ecological methods to prevent plant disease, pests, and erosion fungal spore and nutrition base; The agent of described biocontrol fungi spore is for cultivating the solid or liquid or the solid and the mixtures of liquids that contain a large amount of biocontrol fungi spores and a small amount of biocontrol fungi viable bacteria filament of gained to the mycota biocontrol fungi.Described nutrition base provides the nutriment of carbon, nitrogen and mineral matter and trace element for growing, produce spore for biocontrol fungi fast.
5, fungus biocontrol agent according to claim 1 is characterized in that described biocontrol fungi spore is a kind of or two kinds or several the combination in conidium, chlamydospore or the ascospore etc. of the biocontrol fungi of mycota with antagonism disease fungi function.
6, the application process of fungus biocontrol agent according to claim 1, it is characterized in that spreading fertilizer over the fields fungus biocontrol agent should be afternoon to carrying out at dusk.
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| CNA2009100958753A CN101480156A (en) | 2009-02-16 | 2009-02-16 | Method for using fungal biocontrol agents |
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| CN200910164188A CN101642036A (en) | 2009-02-16 | 2009-08-05 | Application process of fungus biocontrol agent |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103004546A (en) * | 2011-09-22 | 2013-04-03 | 庞占琴 | Nectarine planting method |
| CN105660255A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method for tobacco rot-not nematodes diseases and biocontrol agent thereof |
| CN105660256A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method for tomato root knot nematodes and biocontrol agent thereof |
| CN105660254A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method and biocontrol agent for banana vasicular wilt |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103875717B (en) * | 2014-04-02 | 2015-05-20 | 湖南省植物保护研究所 | Method for promoting orchid to grow and preventing diseases |
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2009
- 2009-02-16 CN CNA2009100958753A patent/CN101480156A/en active Pending
- 2009-08-05 CN CN200910164188A patent/CN101642036A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004546A (en) * | 2011-09-22 | 2013-04-03 | 庞占琴 | Nectarine planting method |
| CN105660255A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method for tobacco rot-not nematodes diseases and biocontrol agent thereof |
| CN105660256A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method for tomato root knot nematodes and biocontrol agent thereof |
| CN105660254A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method and biocontrol agent for banana vasicular wilt |
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