CN104396886A - Callosobruchus maculatus larva large-scale culturing method - Google Patents
Callosobruchus maculatus larva large-scale culturing method Download PDFInfo
- Publication number
- CN104396886A CN104396886A CN201410566545.9A CN201410566545A CN104396886A CN 104396886 A CN104396886 A CN 104396886A CN 201410566545 A CN201410566545 A CN 201410566545A CN 104396886 A CN104396886 A CN 104396886A
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- Prior art keywords
- callosobruchus maculatus
- larva
- maculatus
- callosobruchus
- soybean
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- 241000907862 Callosobruchus maculatus Species 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 9
- 238000012258 culturing Methods 0.000 title abstract 7
- 239000002245 particle Substances 0.000 claims abstract description 26
- 244000068988 Glycine max Species 0.000 claims abstract description 22
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 22
- 241000238631 Hexapoda Species 0.000 claims abstract description 18
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 13
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 13
- 238000009395 breeding Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 210000004681 ovum Anatomy 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 abstract description 6
- 235000013305 food Nutrition 0.000 abstract description 6
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 5
- 238000002224 dissection Methods 0.000 description 3
- 241000907223 Bruchinae Species 0.000 description 2
- 241000254173 Coleoptera Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 240000004922 Vigna radiata Species 0.000 description 2
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 2
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000010523 Cicer arietinum Nutrition 0.000 description 1
- 244000045195 Cicer arietinum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 240000001417 Vigna umbellata Species 0.000 description 1
- 235000011453 Vigna umbellata Nutrition 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a callosobruchus maculatus larva large-scale culturing method, and relates to the technical field of natural enemy insect large-scale production. The method comprises the following steps: first-grade commodity soybean is crushed into particles with a size of 0.3cm*0.4cm; the particles are placed into a plastic fresh-keeping box with a size of 25cm*15cm*6cm, wherein a particle stacking thickness is 2.0cm; callosobruchus maculatus adults are placed into the plastic fresh-keeping box with the soybean particles, and the box is placed into a 28 DEG C insect incubator where the callosobruchus maculatus adults are subjected to 5 days of culturing without light, until the callosobruchus maculatus adults spawn on the soybean particles; after the 5 days, the callosobruchus maculatus adults are removed by using a 6-mesh sieve, and the soybean particles with callosobruchus maculatus eggs are placed into the plastic fresh-keeping box; the box is placed into a 30 DEG C insect incubator where the eggs are subjected to 10 days of culturing without light; after the 10 days, when a large number of callosobruchus maculatus larvae appear, the callosobruchus maculatus larvae are separated from the soybean particles with a 8-mesh sieve, and the larvae are fetched for later use. The method has the following advantages: a link of manually removing the feeding food for acquiring the larvae is eliminated; culturing cost is low; callosobruchus maculatus larva culturing efficiency is high; and a technical support is provided for realizing callosobruchus maculatus larva large-scale culturing.
Description
Technical field:
The present invention relates to a kind of Callosobruchus maculatus larva large-scale breeding method, Callosobruchus maculatus larva is used for predatory natural enemy insect of feeding, and belongs to natural enemy insect large-scale production technical field.
Background technology:
Predatory natural enemy insect is the controlling elements of object noxious insect population control, and its large-scale production is the important channel realizing the sustainable control of harmful insect.Callosobruchus maculatus larva is the main fed food of many predatory natural enemy insects, and its large-scale production is the key realizing predatory natural enemy insect plant modification.Callosobruchus maculatus is a kind of small insects, belongs to coleoptera (Coleoptera), Bruchidae (Bruchidae).Callosobruchus maculatus larva mainly takes food the beans such as red bean, mung bean, chickpea, soybean and pea.Callosobruchus maculatus larva individuality is little, and in the cotyledon of the beans such as mung bean, the growth of moth food, forms adult of future generation until grow.Adopt beans to raise Callosobruchus maculatus larva, normally after Callosobruchus maculatus larva grows to a certain size, hand-dissection's beans takes out larva.This raising method cost of labor is high, and it is low that Callosobruchus maculatus larva obtains efficiency, is difficult to the large-scale production realizing Callosobruchus maculatus larva.The technology of the present invention adopts broken beans to raise Callosobruchus maculatus; Callosobruchus maculatus larva is allowed to get out beans voluntarily; decrease and dissect the raising link that beans takes out Callosobruchus maculatus larva; significantly reduce Callosobruchus maculatus larva raising cost; greatly improve Callosobruchus maculatus larva raising efficiency, for Callosobruchus maculatus larva scale factory farming provides technical support.
By literature search, the open report identical with the present invention is had no.
Summary of the invention:
The object of the invention is to improve Callosobruchus maculatus larva raising efficiency, provide a kind of Callosobruchus maculatus larva large-scale breeding method, for predatory natural enemy insect plant modification provides technical support.
Callosobruchus maculatus larva large-scale breeding method of the present invention, concrete steps are as follows:
1. first-class commodity soybean is crushed to the particle of 0.3 × 0.4cm size by food preparation cracker, this soybean particle is put into the plastic preserving box of 25 × 15 × 6cm, and particle packing thickness is 1.5-2.0cm;
2. egg reproduction puts into the Callosobruchus maculatus adult just sprouted wings the plastic preserving box filling soybean particle, each 20 of female worm male worm placed by every box, cover plastic preserving box lid, without illumination cultivation 5 days in the insect incubator of being inserted 28 DEG C of temperature, treat Callosobruchus maculatus adult egg reproduction on soybean particle;
3. larva raising is after the 5th day, the above-mentioned plastic preserving box filling Callosobruchus maculatus adult is taken out, remove Callosobruchus maculatus adults with 6 object sieves, the soybean particle with Callosobruchus maculatus ovum is put back in plastic preserving box, put into insect incubator that temperature is set to 28-30 DEG C without illumination cultivation 10 days;
5. after larva obtains the 10th day, Callosobruchus maculatus larva and beans to the Callosobruchus maculatus inspection in above-mentioned plastic preserving box once, after seeing that a large amount of Callosobruchus maculatus larva gets out beans, are separated with 8 object sieves by every day, obtain Callosobruchus maculatus larva for subsequent use.
The invention has the advantages that:
1. decrease hand-dissection's beans to obtain the raising link of Callosobruchus maculatus larva, save cost of labor, improve Callosobruchus maculatus larva raising efficiency, its feeding efficiency is 100 times of artificial feeding efficiency.
2. the method is simple to operate, and cost is low, contributes to realizing the scale of Callosobruchus maculatus larva raising and factorial praluction.
Specific implementation method:
The concrete steps of Callosobruchus maculatus larva large-scale breeding method of the present invention are as follows:
1. first-class commodity soybean is crushed to the particle of 0.3 × 0.4cm size by food preparation cracker, the soybean particle after this pulverizing is put into the plastic preserving box of 25 × 15 × 6cm, and particle packing thickness is 2.0cm;
2. Adult worms producting eggs puts into the aforementioned plastic preserving box filling soybean particle the Callosobruchus maculatus adult just sprouted wings, each 20 of every box female worm male worm, then plastic preserving box lid is covered, crisper to be inserted in insect incubator that temperature is set to 28 DEG C without illumination cultivation 5 days, treat that Callosobruchus maculatus adult lays eggs on soybean particle;
3. larva raising is after the 5th day, the above-mentioned plastic preserving box filling Callosobruchus maculatus adult is taken out, with 6 object sieves, Callosobruchus maculatus adult is removed, soybean particle with Callosobruchus maculatus ovum is put into plastic preserving box, cover lid, puts into insect incubator that temperature is set to 30 DEG C without illumination cultivation 10 days;
5. after larva obtains the 10th day, Callosobruchus maculatus larva and soybean particle to the above-mentioned plastic preserving box inspection filling Callosobruchus maculatus once, after seeing that Callosobruchus maculatus larva in enormous quantities out, are separated with 8 object sieves by every day, and it is for subsequent use to take out Callosobruchus maculatus larva.
The method is used for Callosobruchus maculatus larva raising by inventor.Facts have proved; completely eliminated the raising link that hand-dissection's beans obtains Callosobruchus maculatus larva; reduce Callosobruchus maculatus larva raising cost of labor, its feeding efficiency is 100 times of artificial feeding efficiency, provides technical support for realizing Callosobruchus maculatus larva scale factory farming.
Claims (1)
1. a Callosobruchus maculatus larva large-scale breeding method, is characterized in that the concrete steps of the method are as follows:
A. with cracker, first-class commodity soybean is crushed to the particle of 0.3 × 0.4cm size, this soybean particle is put into the plastic preserving box of 25 × 15 × 6cm, particle packing thickness is 2.0cm;
B. the Callosobruchus maculatus adult just sprouted wings is put into the plastic preserving box filling soybean particle, each 20 of female worm male worm placed by every box, cover plastic preserving box lid, without illumination cultivation 5 days in the insect incubator of being inserted 28 DEG C of temperature, treat Callosobruchus maculatus adult egg reproduction on soybean particle;
C. after the 5th day, the above-mentioned plastic preserving box filling Callosobruchus maculatus adult is taken out, remove Callosobruchus maculatus adults with 6 object sieves, the soybean particle with Callosobruchus maculatus ovum is put back in plastic preserving box, put into insect incubator that temperature is set to 30 DEG C without illumination cultivation 10 days;
D. larva obtains: after the 10th day, Callosobruchus maculatus larva and beans to the Callosobruchus maculatus inspection in above-mentioned plastic preserving box once, after seeing that a large amount of Callosobruchus maculatus larva gets out beans, are separated with 8 object sieves by every day, obtains Callosobruchus maculatus larva for subsequent use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410566545.9A CN104396886B (en) | 2014-10-22 | 2014-10-22 | A kind of Callosobruchus maculatus larva large-scale breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410566545.9A CN104396886B (en) | 2014-10-22 | 2014-10-22 | A kind of Callosobruchus maculatus larva large-scale breeding method |
Publications (2)
| Publication Number | Publication Date |
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| CN104396886A true CN104396886A (en) | 2015-03-11 |
| CN104396886B CN104396886B (en) | 2016-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410566545.9A Expired - Fee Related CN104396886B (en) | 2014-10-22 | 2014-10-22 | A kind of Callosobruchus maculatus larva large-scale breeding method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284741A (en) * | 2015-11-06 | 2016-02-03 | 天津拓华生物科技有限责任公司 | Mass propagation method for larvae of callosobruchus chinensis linnaeus |
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601375A (en) * | 2008-06-12 | 2009-12-16 | 中国农业科学院植物保护研究所 | A kind of artificial fecundation method of aphid |
| KR20110032527A (en) * | 2009-09-23 | 2011-03-30 | 김학남 | Mass production system of the natural enemy for banker plants |
| CN102524194A (en) * | 2012-02-26 | 2012-07-04 | 新疆农业大学 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
| CN103828576A (en) * | 2014-01-27 | 2014-06-04 | 中国农业科学院作物科学研究所 | Identification method of resistance of broad beans to callosobruchus chinensis |
-
2014
- 2014-10-22 CN CN201410566545.9A patent/CN104396886B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601375A (en) * | 2008-06-12 | 2009-12-16 | 中国农业科学院植物保护研究所 | A kind of artificial fecundation method of aphid |
| KR20110032527A (en) * | 2009-09-23 | 2011-03-30 | 김학남 | Mass production system of the natural enemy for banker plants |
| CN102524194A (en) * | 2012-02-26 | 2012-07-04 | 新疆农业大学 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
| CN103828576A (en) * | 2014-01-27 | 2014-06-04 | 中国农业科学院作物科学研究所 | Identification method of resistance of broad beans to callosobruchus chinensis |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284741A (en) * | 2015-11-06 | 2016-02-03 | 天津拓华生物科技有限责任公司 | Mass propagation method for larvae of callosobruchus chinensis linnaeus |
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104396886B (en) | 2016-08-31 |
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Granted publication date: 20160831 Termination date: 20171022 |