CN102524194A - Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests - Google Patents
Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests Download PDFInfo
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- CN102524194A CN102524194A CN2012100437921A CN201210043792A CN102524194A CN 102524194 A CN102524194 A CN 102524194A CN 2012100437921 A CN2012100437921 A CN 2012100437921A CN 201210043792 A CN201210043792 A CN 201210043792A CN 102524194 A CN102524194 A CN 102524194A
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Abstract
The invention discloses a method for artificially propagating pyemotes bark beetle and the application in biological control of bark beetle pests. The method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis comprises the steps as follows: firstly, a host that is Callosobruchus chinensis is produced; secondly, protospecies of pyemotes bark beetle is prepared; and thirdly, pyemotes bark beetle is propagated. The method and the application have the benefits as follows: the difficult problem of propagating pyemotes bark beetle to replace the host is solved; pyemotes bark beetle can be propagated in large quantities; the method is simple and convenient to operate; large-scale production can be carried out; the method is applicable to control Scolytus seulensis Murayama pests on forests and fruit trees such as apricot trees, peach trees and plum trees; the problem that the forests and the fruit trees such as the apricot trees, the peach trees and the plum trees are damaged by the Scolytus seulensis Murayama pests and whole branches or entire trees die back further because the parasitism rate of pyemotes bark beetle is lower can be solved; therefore, the requirements of forestry and fruit Industry production can be satisfied, pollution on the environment cannot be caused, and the method is an effective technological means for controlling the Scolytus seulensis Murayama pests in an environmental protection manner.
Description
Technical field
the present invention relates to the artificial method that expands numerous bark beetle Pu mite and reach the technical field at the bark beetle biological control of insect pests, are a kind of artificial expand method of numerous bark beetle Pu mite and application in the bark beetle biological control of insect pests.
Background technology
The crinosity bark beetle (
Scolytus seulensisMurayama) belong to Scolytidae; In the equal scope in Xinjiang, generation is arranged all; Be with generation comparatively serious with Aksu, Kuerle, Luntai County, Kuqa County one, branches of fruit trees such as main harm apricot, peach, Lee cause the phloem conducting tissue to damage; Hinder the transportation of moisture and nutriment, finally cause training or complete stool withered withered.Cause the tree body to dredge tissue die.
" Xinjiang agricultural science and technology "; 1993; (5): made following report by medium " generation of the little stupid worm of crinosity and the integrated control technique " literary composition delivered of Lee in the 215th page to the 216th page: the storehouse car of the mid-80 crinosity bark beetle Aksu Prefecture in Xinjiang, Shaya, newly and just generation is arranged, car is to 0.25 * 10 in the storehouse
4
Hm
2
The apricot garden investigation of being injured serious on average has worm strain rate to reach 17.5%, and withered rate reaches 9.23%, and endangering serious apricot garden has worm strain rate up to 88% to 100%, and withered rate reaches 52% to 81%.
Following report made in " generation and the study on prevention of apricot crinosity bark beetle " literary composition of being delivered by Zhao Linzhong etc. in
" plant protection technology and popularization " the 1995 (2): the 11st page to the 12nd page: Hami Prefecture's strain rate of being injured in the apricot garden in 1991-1999 years reaches 8.2% to 42.6%, and the production loss rate is up to 64.96% to 78.14%.
" plant protection " 2009; Following report made in " Kaxgar Prefecture crinosity bark beetle takes place and causes harm rule " literary composition of being delivered by Li Hong etc. in the 35 (6): the 135th page to the 138th page: Kaxgar Prefecture is caused harm by the crinosity bark beetle 2005 to cause death the apricot less than in the apricot garden, to cause harm up to 25.9% in 1%, 2008 year.The Luntai County since two thousand two, the crinosity bark beetle generation area and the extent of injury constantly increase.Whole county apricot bark beetle generation area in 2007 is up to 4.5 ten thousand mu according to statistics, and wherein the old apricot garden hazard ratio of life in 20 years is up to 80%, has caused more than ten ten thousand strain (branch) apricots withered, and the healthy and sustainable development of whole county apricot industry has been constituted very big threat.
are present, and the main method of preventing and treating crinosity bark beetle insect has two kinds, and a kind of is to kill insect with chemical insecticide, because the crinosity bark beetle is to bore moth harm, increased difficulty therefore for effectively control; Another kind is to utilize the natural enemy of insect to eliminate destructive insects; Found that at natural world the natural enemy of parasitic crinosity bark beetle is a bark beetle Pu mite; This mite nature parasitic rate is 10% to 30%, but this far can not stop the breeding of crinosity bark beetle insect, thereby it is dead to cause training or complete stool to dry up; Have a strong impact on the development of fruit-bearing forests such as apricot, peach, Lee industry, also influenced expanding economy.
are comparatively difficult owing to crinosity bark beetle, navel abdomen bark beetle larva raising get up, and the natural parasitic rate of bark beetle Pu mite is lower again, and therefore a large amount of breeding bark beetle Pu mites are difficult, thereby also influence with bark beetle Pu acarid control crinosity bark beetle.
Summary of the invention
It is a kind of with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil that
the present invention provides, and overcome the deficiency of prior art, solved the problem that can not breed bark beetle Pu mite in a large number.
It is a kind of with the application of the artificial numerous bark beetle Pu mite of expansion of adzuki bean weevil in the bark beetle biological control of insect pests that
the present invention also provides; Overcome the deficiency of prior art, it is lower and cause fruit trees such as apricot, peach, Lee to be made its training or the withered dead problem of complete stool by crinosity bark beetle pest damage to solve bark beetle Pu mite parasitic rate.
One of technical scheme of the present invention obtains in the following manner: a kind of with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil, it is undertaken by following step:
The first step substitutes the host adzuki bean weevil and produces: the access of adzuki bean weevil adult is filled in the crisper of mung bean or cowpea; After treating that beans surface product has ovum; Raising is 20 ℃ to 32 ℃ of temperature, under relative moisture 40% to 90% condition, sees that at bean flour larva stings into the emergence hole of 1 circle, plants skin and keep when transparent; Transparent kind skin is needled, prepared to insert bark beetle Pu mite;
The preparation of the second step bark beetle Pu mite original seed: the bark beetle Pu mite on field acquisition crinosity bark beetle larva or the pupal cell, place culture dish, in 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, to raise 6 days to 13 days, the bark beetle Pu mite that raising obtains is as original seed;
The expansion of bark beetle Pu mite of
the 3rd step is numerous: will in have transparent kind of skin of big instar larvae of adzuki bean weevil and pupa to be needled mung bean insert in the gauze bag; With bark beetle Pu mite original seed and alternative host adzuki bean weevil larva or the access of 1: 4 ratio of pupa; The gauze bag of inoculation is inserted in the crisper that has husky net; Put into 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time again, raised 6 days to 13 days, so just breed out a large amount of bark beetle Pu mites.
Be further optimization and/or selection below to one of technique scheme:
The diameter of
above-mentioned culture dish is 9 centimetres.
scribble vaseline around above-mentioned culture dish.
The length of
above-mentioned crisper is that 30 centimetres, width are 20 centimetres, highly are 8 centimetres, the husky net of lid and both sides band.
in above-mentioned the 3rd step, the length of gauze bag is 5, width is 7 centimetres, and the addition of mung bean is 1/4th a capacity of gauze bag, and the gauze bag is 80 order to 100 purposes.
Two of
technical scheme of the present invention obtains in the following manner: a kind of according to the above-mentioned application of bark beetle Pu mite in the bark beetle biological control of insect pests that obtains with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil; When a large amount of output of bark beetle Pu mite of breeding, gauze bag one side is tufted into apricot and has by the brill channel of bark beetle.
Be two further optimization and/or selection below to technique scheme:
above-mentioned bark beetle Pu mite in the open-air time that discharges of South Sinkiang, Xinjiang is: every year by the end of April to 6 months at the beginning of or by the end of August to by the end of September.
the invention has the beneficial effects as follows: the invention solves and expand the difficult problem that breeding bark beetle Pu mite substitutes the host; A large amount of breeding bark beetle Pu mites have been realized; Easy and simple to handle, can carry out large-scale production, be applied to prevent and treat apricot, peach, Li Denglin, fruit tree crinosity bark beetle insect; It is lower and cause apricot, peach, Li Denglin, fruit tree to be made its training or the withered dead problem of complete stool by crinosity bark beetle pest damage to solve bark beetle Pu mite parasitic rate; Thereby having satisfied the needs that forests and fruit industry is produced, and environment has not been produced pollution, is the effective technology means that environmental protection prevents and treats crinosity bark beetle insect.
Embodiment
the present invention does not receive the restriction of following embodiment, can confirm concrete embodiment according to the technical scheme and the actual conditions of the invention described above.
Below in conjunction with embodiment the present invention is done further argumentation:
Embodiment 1, and the artificial method that expands numerous bark beetle Pu mite of this usefulness adzuki bean weevil is undertaken by following step:
The first step substitutes the host adzuki bean weevil and produces: the access of adzuki bean weevil adult is filled in the crisper of mung bean or cowpea; After treating that beans surface product has ovum; Raising is 20 ℃ to 32 ℃ of temperature, under relative moisture 40% to 90% condition, sees that at bean flour larva stings into the emergence hole of 1 circle, plants skin and keep when transparent; Transparent kind skin is needled, prepared to insert bark beetle Pu mite;
The preparation of the second step bark beetle Pu mite original seed: gather the bark beetle Pu mite on crinosity bark beetle larva or the pupal cell from open-air (generally can from the apricot that crinosity bark beetle insect is arranged); Place culture dish; In 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time; Raised 6 days to 13 days, the bark beetle Pu mite that raising obtains is as original seed;
The expansion of bark beetle Pu mite of
the 3rd step is numerous: will in have transparent kind of skin of big instar larvae of adzuki bean weevil and pupa to be needled mung bean insert in 80 order to the 100 purpose gauze bags; With bark beetle Pu mite original seed and alternative host adzuki bean weevil larva or the access of 1: 4 ratio of pupa; The gauze bag of inoculation is inserted in the crisper that has husky net; Put into 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time again, raised 6 days to 13 days, so just breed out a large amount of bark beetle Pu mites.
embodiment 2, be with the difference of embodiment 1: the diameter of the culture dish of embodiment 2 is 9 centimetres.
embodiment 3 is with the difference of embodiment 2: scribble vaseline around the culture dish of embodiment 3.
embodiment 4 is with the difference of embodiment 1 to embodiment 3: the length of the crisper of embodiment 4 is that 30 centimetres, width are 20 centimetres, highly are 8 centimetres, the husky net of lid and both sides band.
embodiment 5; Be with the difference of embodiment 1 to embodiment 4: in the 3rd step of embodiment 5; The length of gauze bag is 5, width is 7 centimetres, and the addition of mung bean is 1/4th a capacity of gauze bag, and the gauze bag is 80 order to 100 purposes.
are inoculated into the bark beetle Pu mite on adzuki bean weevil larva and the pupal cell in embodiment 1 to 5, its parasitic rate has all reached 100%.
adzuki bean weevil larva can eat into the various beans of food, likes food mung bean, red bean and cowpea most, and material is easy to get; In 4 generations in generation to 5, took place in adzuki bean weevil 1 year, breeds in the suitable or storehouse like environment, can take place in 1 year 11 generations; The anniversary breeding, every completion 1 generation need 20 days to 67 days.4 days to 25 days female adult worm life-span, male imago 5 days to 33 days.
therefore, the present invention has not only solved the problem of a large amount of breeding bark beetle Pu mites, and simple to operation.
embodiment 6; Be with the difference of embodiment 1 to embodiment 5: embodiment 6 is with embodiment 1 to embodiment 5 application of resulting bark beetle Pu mite in the bark beetle biological control of insect pests; When a large amount of output of bark beetle Pu mite of breeding, gauze bag one side is tufted into apricot and has by the brill channel of bark beetle.
embodiment 7 is with the difference of embodiment 6: the bark beetle Pu mite of embodiment 7 in the open-air time that discharges of South Sinkiang, Xinjiang is: every year by the end of April to 6 months at the beginning of or by the end of August to by the end of September.
are applied in embodiment 6 and embodiment 7 on the apricot fruit tree; Bark beetle Pu mite is at the parasitic rate average out to 45.2% of crinosity bark beetle; Greatly improved its parasitic rate, effectively reduced the harm of crinosity bark beetle and cause the limb of apricot withered, very safe to the apricot fruit.
Claims (8)
1. one kind with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil, it is characterized in that being undertaken by following step:
The first step substitutes the host adzuki bean weevil and produces: the access of adzuki bean weevil adult is filled in the crisper of mung bean or cowpea; After treating that beans surface product has ovum; Raising is 20 ℃ to 32 ℃ of temperature, under relative moisture 40% to 90% condition, sees that at bean flour larva stings into the emergence hole of 1 circle, plants skin and keep when transparent; Transparent kind skin is needled, prepared to insert bark beetle Pu mite;
The preparation of the second step bark beetle Pu mite original seed: the bark beetle Pu mite on field acquisition crinosity bark beetle larva or the pupal cell, place culture dish, in 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, to raise 6 days to 13 days, the bark beetle Pu mite that raising obtains is as original seed;
The 3rd the step bark beetle Pu mite expansion numerous: will in have transparent kind of skin of big instar larvae of adzuki bean weevil and pupa to be needled mung bean insert in the gauze bag; With bark beetle Pu mite original seed and alternative host adzuki bean weevil larva or the access of 1: 4 ratio of pupa; The gauze bag of inoculation is inserted in the crisper that has husky net; Put into 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time again, raised 6 days to 13 days, so just breed out a large amount of bark beetle Pu mites.
2. according to claim 1 with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil, the diameter that it is characterized in that culture dish is 9 centimetres.
3. according to claim 2 with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil, it is characterized in that scribbling vaseline around the culture dish.
4. according to claim 1 or 2 or 3 described with the artificial methods that expand numerous bark beetle Pu mite of adzuki bean weevil, the length that it is characterized in that crisper is that 30 centimetres, width are 20 centimetres, highly are 8 centimetres, lid and both sides band sand net.
5. according to claim 1 or 2 or described with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil; It is characterized in that in the 3rd step; The length of gauze bag is 5, width is 7 centimetres, and the addition of mung bean is 1/4th a capacity of gauze bag, and the gauze bag is 80 order to 100 purposes.
6. according to claim 4 with the artificial method that expands numerous bark beetle Pu mite of adzuki bean weevil; It is characterized in that in the 3rd step; The length of gauze bag is 5, width is 7 centimetres, and the addition of mung bean is 1/4th a capacity of gauze bag, and the gauze bag is 80 order to 100 purposes.
7. one kind according to the arbitrarily described application of bark beetle Pu mite in the bark beetle biological control of insect pests that obtains with the method for the numerous bark beetle Pu mite of the artificial expansion of adzuki bean weevil of claim in the claim 1 to 6; It is characterized in that when a large amount of output of bark beetle Pu mite of breeding, gauze bag one side is tufted into apricot and has by the brill channel of bark beetle.
8. 7 application of described bark beetle Pu mite in the bark beetle biological control of insect pests as requested is characterized in that bark beetle Pu mite in the open-air time that discharges of South Sinkiang, Xinjiang is: every year by the end of April to 6 months at the beginning of or by the end of August to by the end of September.
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| CN201210043792.1A CN102524194B (en) | 2012-02-26 | 2012-02-26 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396886A (en) * | 2014-10-22 | 2015-03-11 | 云南大学 | Callosobruchus maculatus larva large-scale culturing method |
| CN105230576A (en) * | 2015-11-06 | 2016-01-13 | 天津拓华生物科技有限责任公司 | Method for mass production of callosobruchus chinensis (linnaeus) larvae through peeled mung beans |
| CN105284741A (en) * | 2015-11-06 | 2016-02-03 | 天津拓华生物科技有限责任公司 | Mass propagation method for larvae of callosobruchus chinensis linnaeus |
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
| CN107279073A (en) * | 2017-08-17 | 2017-10-24 | 广州市林业和园林科学研究院 | A kind of coconut knits the indoor feeding method of moth |
| CN114794026A (en) * | 2022-05-10 | 2022-07-29 | 广西壮族自治区林业科学研究院 | Method for preventing and treating camellia oleifera pest namely plum blossom moth by using pyemotes giganteus for expanding propagation of old larvae of galleria mellonella |
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2012
- 2012-02-26 CN CN201210043792.1A patent/CN102524194B/en not_active Expired - Fee Related
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396886A (en) * | 2014-10-22 | 2015-03-11 | 云南大学 | Callosobruchus maculatus larva large-scale culturing method |
| CN104396886B (en) * | 2014-10-22 | 2016-08-31 | 云南大学 | A kind of Callosobruchus maculatus larva large-scale breeding method |
| CN105230576A (en) * | 2015-11-06 | 2016-01-13 | 天津拓华生物科技有限责任公司 | Method for mass production of callosobruchus chinensis (linnaeus) larvae through peeled mung beans |
| CN105284741A (en) * | 2015-11-06 | 2016-02-03 | 天津拓华生物科技有限责任公司 | Mass propagation method for larvae of callosobruchus chinensis linnaeus |
| CN105230576B (en) * | 2015-11-06 | 2017-12-26 | 天津拓华生物科技有限责任公司 | A kind of method using mung bean large-scale production Callosobruchus chinensis larva of peeling |
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
| CN107279073A (en) * | 2017-08-17 | 2017-10-24 | 广州市林业和园林科学研究院 | A kind of coconut knits the indoor feeding method of moth |
| CN114794026A (en) * | 2022-05-10 | 2022-07-29 | 广西壮族自治区林业科学研究院 | Method for preventing and treating camellia oleifera pest namely plum blossom moth by using pyemotes giganteus for expanding propagation of old larvae of galleria mellonella |
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