CN105230576A - Method for mass production of callosobruchus chinensis (linnaeus) larvae through peeled mung beans - Google Patents
Method for mass production of callosobruchus chinensis (linnaeus) larvae through peeled mung beans Download PDFInfo
- Publication number
- CN105230576A CN105230576A CN201510756320.4A CN201510756320A CN105230576A CN 105230576 A CN105230576 A CN 105230576A CN 201510756320 A CN201510756320 A CN 201510756320A CN 105230576 A CN105230576 A CN 105230576A
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- Prior art keywords
- mung bean
- bean
- adzuki
- bag
- larva
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- 240000004922 Vigna radiata Species 0.000 title claims abstract description 62
- 235000010721 Vigna radiata var radiata Nutrition 0.000 title claims abstract description 62
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 title claims abstract description 61
- 241001313742 Callosobruchus chinensis Species 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 239000002002 slurry Substances 0.000 claims abstract description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 7
- 229920001817 Agar Polymers 0.000 claims abstract description 6
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims abstract description 6
- 229920002472 Starch Polymers 0.000 claims abstract description 6
- 239000008272 agar Substances 0.000 claims abstract description 6
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 6
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims abstract description 6
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims abstract description 6
- 239000008107 starch Substances 0.000 claims abstract description 6
- 235000019698 starch Nutrition 0.000 claims abstract description 6
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 12
- 238000011031 large-scale manufacturing process Methods 0.000 claims description 11
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 4
- 230000007613 environmental effect Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract 2
- 241000238631 Hexapoda Species 0.000 description 5
- 241000907223 Bruchinae Species 0.000 description 2
- 241000254173 Coleoptera Species 0.000 description 2
- 241000382353 Pupa Species 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241001143309 Acanthoscelides obtectus Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 241000219977 Vigna Species 0.000 description 1
- 235000010716 Vigna mungo Nutrition 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
- 240000001417 Vigna umbellata Species 0.000 description 1
- 235000011453 Vigna umbellata Nutrition 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a method for mass production of callosobruchus chinensis (linnaeus) larvae through peeled mung beans. The method includes the following steps that 1, wrapped slurry liquid is prepared, wherein a mixture of starch, agar powder and carboxymethylcellulose powder of the equal mass ratio is blended into the wrapped slurry liquid through warm water of 50-100 DEG C, and the concentration of the mixture is 1%-10%; 2, slurry wrapping is conducted; 3, the callosobruchus chinensis (linnaeus) larvae are bred; 4, the callosobruchus chinensis (linnaeus) larvae are collected. According to the method, a large amount of labor for stripping the larvae does not need to be consumed, mechanical damage to the larvae can be avoided, mass reproduction cost of the callosobruchus chinensis (linnaeus) larvae is lowered, and large-scale cultivation of the callosobruchus chinensis (linnaeus) larvae is made possible.
Description
Technical field
The present invention relates to the artificial breeding method of a kind of insect, particularly relate to a kind of method utilizing decortication mung bean amount reproduction adzuki bean weevil larva.
Background technology
Adzuki bean weevil (Callosobruchuschinensis (Linnaeus)), is under the jurisdiction of coleoptera (Coleoptera), Bruchidae (Bruchidae).Host comprises Kidney bean, cowpea, French beans, pea, broad bean, mung bean, red bean etc.With young wormy legumen, food evil beans, or the beans of moth food storage in storehouse are important grain storage insects.
This kind of larva body is little, resistance ability is weak, body wall is soft, rich in protein, it is the Hosts that parasite and predator are good, but bean weevil larva lives in mung bean seed throughout one's life, its growth conditions can not be observed from outside, in addition adzuki bean weevil is short for the history of life, cause generation overlap phenomenon in extensive feeding process serious, same batch of growth is irregular, the mung bean that artificial screening contains mature larva is wasted time and energy, the workload manually larva being separated mung bean is unimaginable especially, adzuki bean weevil larva production cost is directly caused to remain high, and large-scale industrial production can not be carried out, have impact on the application of adzuki bean weevil larva as natural enemy Hosts.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of method utilizing decortication mung bean large-scale production adzuki bean weevil larva, the method is with serous coat parcel decortication mung bean, the nature of simulation mung bean, lures adzuki bean weevil Adult worms producting eggs into, and larva is normal growth in decortication mung bean, after aging to larva, bag slurry mung bean is infiltrated in water, dissolves serous coat, adzuki bean weevil larva just can be made naturally to depart from beans.This method is easy and simple to handle, with low cost, can obtain the adzuki bean weevil larva departing from beans easily in a large number, the large-scale production of adzuki bean weevil is achieved.
The technical solution adopted in the present invention is:
Utilize a method for decortication mung bean large-scale production adzuki bean weevil larva, comprise the following steps:
1), make bag slurries: the warm water using 50-100 DEG C, by starch, agar powder, carboxymethyl cellulose powder etc. the mixture of mass ratio, be deployed into bag slurries; The concentration of mixture is 1% ~ 10%;
2), bag slurry;
3), the breeding of adzuki bean weevil larva;
4), the collection of adzuki bean weevil larva.
Further, described bag slurry, step is: mung bean of peeling is put into bag slurries, stirred, make decortication mung bean fully contact bag slurries, pulled out by decortication mung bean afterwards, dry in the shade after cleaning, and makes it evenly wraps up one deck serous coat, i.e. obtained bag slurry mung bean.
Further, the breeding of described wherein adzuki bean weevil larva, step is: by adzuki bean weevil adult (female-male proportion (1 ~ 3): 1), mass ratio access with 1% ~ 10% is in the decortication mung bean of bag slurry, being placed in environmental temperature is 25-30 DEG C, relative moisture 65% ~ 85%, cultivates 10-25 days.
Further, the collection of described adzuki bean weevil larva, step is, is soaked in water by bag slurry mung bean, stirs, make serous coat dissolve, come off, make adzuki bean weevil larva wherein naturally depart from beans, then by 8-20 mesh screen, remove mung bean grain, can obtain adzuki bean weevil larva.
The advantage that the present invention has and beneficial effect:
It is inner that adzuki bean weevil all lived in mung bean seed before adult, want the larva obtaining departing from beans, manual removal can only be relied on, human cost is high, cause mechanical damage probability very high to larva in peeling off simultaneously, cannot ensure larva yield, make the production cost of adzuki bean weevil larva high, production technology can not large-scale application.
The technical scheme that the present invention proposes, the habit that green gram spermoderm does pupa room is close to before make use of adzuki bean weevil larvae pupation, by carrying out the mode of wrapping slurry to decortication mung bean, the nature of simulation mung bean, lures Adult worms producting eggs into, treat that larvae development is ripe, be close to serous coat when doing pupa room, be soaked in water bag slurry mung bean, dissolved by serous coat, make larva naturally depart from mung bean, well solve the collection problem of adzuki bean weevil larva.The program neither needs at substantial manual removal larva, also can not cause mechanical damage to larva, reduces the cost that adzuki bean weevil larva breeds on a large scale, makes the large-scale cultivation of adzuki bean weevil larva become possibility.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, but do not limit protection scope of the present invention.
Embodiment 1
Utilize a method for decortication mung bean large-scale production adzuki bean weevil larva, comprise the following steps:
By starch, agar powder, carboxymethyl cellulose powder etc. quality than mixture, the water-soluble solution of 75 DEG C of the mass ratio with 5%, makes bag slurries.Mung bean of being peeled by 1kg immerses in bag slurries, fully stirs, mung bean is fully contacted with bag slurries, pulls decortication mung bean afterwards out, dry in the shade, and makes bag slurry mung bean.By be inoculated into the pairing adzuki bean weevil adult 50g (mass ratio of 5%) of sex ration 1:1 be placed with bag slurry mung bean insect box in, be placed in 25 DEG C, in the environment of relative moisture 65%, lucifuge cultivates 15 days.After 15 days, be immersed in the water by bag slurry mung bean, stir, serous coat is come off, and adzuki bean weevil larva departs from beans naturally, then uses 20 mesh screen, removes mung bean, collects adzuki bean weevil larva 221.5g, about 36500.
Embodiment 2
Utilize a method for decortication mung bean large-scale production adzuki bean weevil larva, comprise the following steps:
By starch, agar powder, carboxymethyl cellulose powder etc. quality than mixture, the water-soluble solution of 100 DEG C of the mass ratio with 10%, makes bag slurries.Mung bean of being peeled by 1kg immerses in bag slurries, fully stirs, mung bean is fully contacted with bag slurries, pulls decortication mung bean afterwards out, dry in the shade, and makes bag slurry mung bean.The adzuki bean weevil adult 10g (mass ratio of 1%) matched with sex ration 3:1 is inoculated into be placed with bag slurry mung bean insect box in, be placed in 30 DEG C, in the environment of relative moisture 85%, lucifuge cultivates 10 days.After 10 days, be immersed in the water by bag slurry mung bean, stir, serous coat is come off, and adzuki bean weevil larva departs from beans naturally, then uses 16 mesh screen, removes mung bean, collects adzuki bean weevil larva 105.3g, about 17500.
Embodiment 3
Utilize a method for decortication mung bean large-scale production adzuki bean weevil larva, comprise the following steps:
By starch, agar powder, carboxymethyl cellulose powder etc. quality than mixture, the water-soluble solution of 50 DEG C of the mass ratio with 1%, makes bag slurries.Mung bean of being peeled by 1kg immerses in bag slurries, fully stirs, mung bean is fully contacted with bag slurries, pulls decortication mung bean afterwards out, dry in the shade, and makes bag slurry mung bean.The adzuki bean weevil adult 100g (mass ratio of 10%) matched with sex ration 2:1 is inoculated into be placed with bag slurry mung bean insect box in, be placed in 28 DEG C, in the environment of relative moisture 75%, lucifuge cultivates 20 days.After 20 days, be immersed in the water by bag slurry mung bean, stir, serous coat is come off, and adzuki bean weevil larva departs from beans naturally, then uses 8 mesh screen, removes mung bean, collects adzuki bean weevil larva 310g, about 51600.
The above is only preferred embodiment of the present invention, not does any pro forma restriction to technical scheme of the present invention.Every above embodiment is done according to technical spirit of the present invention any simple modification, equivalent variations and modification, all still belong in the scope of technical scheme of the present invention.
Claims (4)
1. utilize a method for decortication mung bean large-scale production adzuki bean weevil larva, it is characterized in that: comprise the following steps:
1), make bag slurries: the warm water using 50-100 DEG C, by starch, agar powder, carboxymethyl cellulose powder etc. the mixture of mass ratio, be deployed into bag slurries; The concentration of mixture is 1% ~ 10%;
2), bag slurry;
3), the breeding of adzuki bean weevil larva;
4), the collection of adzuki bean weevil larva.
2. a kind of method utilizing decortication mung bean large-scale production adzuki bean weevil larva according to claim 1, it is characterized in that: described bag slurry, step is: after mung bean of peeling is cleaned, put into bag slurries, stir, make decortication mung bean fully contact bag slurries, afterwards decortication mung bean is pulled out, dry in the shade, make it evenly wraps up one deck serous coat, be i.e. obtained bag slurry mung bean.
3. a kind of method utilizing decortication mung bean large-scale production adzuki bean weevil larva according to claim 1 and 2, it is characterized in that: the breeding of described wherein adzuki bean weevil larva, step is: by adzuki bean weevil adult (female-male proportion (1 ~ 3): 1), mass ratio access with 1% ~ 10% is in the decortication mung bean of bag slurry, being placed in environmental temperature is 25-30 DEG C, relative moisture 65% ~ 85%, cultivates 10-25 days.
4. a kind of method utilizing decortication mung bean large-scale production adzuki bean weevil larva according to any one of claim 1-3, it is characterized in that: the collection of described adzuki bean weevil larva, step is, bag slurry mung bean is soaked in water, stirs, make serous coat dissolve, come off, adzuki bean weevil larva is wherein made naturally to depart from beans, again by 8-20 mesh screen, remove mung bean grain, adzuki bean weevil larva can be obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510756320.4A CN105230576B (en) | 2015-11-06 | 2015-11-06 | A kind of method using mung bean large-scale production Callosobruchus chinensis larva of peeling |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510756320.4A CN105230576B (en) | 2015-11-06 | 2015-11-06 | A kind of method using mung bean large-scale production Callosobruchus chinensis larva of peeling |
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| Publication Number | Publication Date |
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| CN105230576A true CN105230576A (en) | 2016-01-13 |
| CN105230576B CN105230576B (en) | 2017-12-26 |
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| CN201510756320.4A Expired - Fee Related CN105230576B (en) | 2015-11-06 | 2015-11-06 | A kind of method using mung bean large-scale production Callosobruchus chinensis larva of peeling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031487A (en) * | 1987-07-29 | 1989-03-08 | 中山大学 | The micro capsule technology of delamination packing clothes |
| EP0390819A4 (en) * | 1987-11-20 | 1992-05-27 | Biosys | Mass production in liquid culture of insect-killing nematodes |
| CN1240461A (en) * | 1996-12-17 | 2000-01-05 | 沃尼尔·朗伯公司 | Polymer film composition for capsules |
| CN102524194A (en) * | 2012-02-26 | 2012-07-04 | 新疆农业大学 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
| CN103385389A (en) * | 2013-07-30 | 2013-11-13 | 天津拓华生物科技有限责任公司 | Rodlike matrix for spawning of thyestilla gebleri, and applications of rodlike matrix |
-
2015
- 2015-11-06 CN CN201510756320.4A patent/CN105230576B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031487A (en) * | 1987-07-29 | 1989-03-08 | 中山大学 | The micro capsule technology of delamination packing clothes |
| EP0390819A4 (en) * | 1987-11-20 | 1992-05-27 | Biosys | Mass production in liquid culture of insect-killing nematodes |
| CN1240461A (en) * | 1996-12-17 | 2000-01-05 | 沃尼尔·朗伯公司 | Polymer film composition for capsules |
| CN102524194A (en) * | 2012-02-26 | 2012-07-04 | 新疆农业大学 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
| CN103385389A (en) * | 2013-07-30 | 2013-11-13 | 天津拓华生物科技有限责任公司 | Rodlike matrix for spawning of thyestilla gebleri, and applications of rodlike matrix |
Non-Patent Citations (1)
| Title |
|---|
| 罗明生等: "《药剂辅料大全》", 31 January 2006, 四川科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
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| CN105230576B (en) | 2017-12-26 |
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Granted publication date: 20171226 Termination date: 20191106 |