CN104365219B - A kind of method that promotes abrus cantoniensis Hance seed germination - Google Patents
A kind of method that promotes abrus cantoniensis Hance seed germination Download PDFInfo
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- CN104365219B CN104365219B CN201410750670.5A CN201410750670A CN104365219B CN 104365219 B CN104365219 B CN 104365219B CN 201410750670 A CN201410750670 A CN 201410750670A CN 104365219 B CN104365219 B CN 104365219B
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Abstract
The invention discloses a kind of method that promotes abrus cantoniensis Hance seed germination, it comprises the collection of seed, the dry and sorting of seed, and seed water causes, seed disinfection and germination. Invention is for the feature of abrus cantoniensis Hance seed, utilizing polyglycol solution to carry out water causes the strong dormancy of releasing abrus cantoniensis Hance seed, utilizes steps such as tedding after-ripening, screening and wet concentration to ensure kernel maturing, plumpness and the uniformity, utilize thimerosal to kill the surface of the seed germ, and suitable germination light warm water condition is provided, promote abrus cantoniensis Hance seed germination. Compare common abrus cantoniensis Hance seed culture method and only have 2% germinating energy and 20% germination percentage, the present invention brings up to more than 9% abrus cantoniensis Hance potentiality of seed, and germination percentage is brought up to more than 75%.
Description
Technical field
The present invention relates to vegetable seeds termination of diapause technical field, particularly a kind of abrus cantoniensis Hance seed germination that promotesMethod.
Technical background
Abrus cantoniensis Hance (AbruscantoniensisHance) is pulse family Abrus climbing shrub, root, complete stool andSeed all can hyoscine, and medicinal material name " Canton love-pea vine " when all herbal medicine can clearing heat and promoting diuresis, Shugan Zhitong, for acute, chronic hepatitis andThe treatment of mastitis. Along with " Herba abri capsule ", " Herba Abri pill ", " Guanule contg. ' Jigucao particle ", " Guanule contg. ' Jigucao electuary " etc.The successful exploitation listing of multiple Chinese patent drug product, market constantly increases the demand of Canton love-pea vine medicinal material, artificial cultivation Guangzhou phaseThinking son is the crucial way of the contradiction between the expansion of solution market demand and wild resource amount die-off.
Abrus cantoniensis Hance seed is tame main propagating materials, but seed permeating properties of seed capsule is poor and raw to embryoThe long mechanical constraint having, causes percentage of seedgermination low, has strong dormancy, and seed difficulty is sprouted to have become and restricted itThe serious problems that expand cultivation and further effectively utilize. In addition, the florescence of abrus cantoniensis Hance is longer, and the fruit phase is longer, causes sameOne batch of fruit pod maturity of gathering is inconsistent, the moisture of differing maturity seed, weight, plumpness, appearance color, germination percentageHave significant difference etc. quality index, seed quality is uneven is also the uneven major reason of emerging while causing artificial cultivation.
At present, the method for removing strong Seeds of Medicinal Plants dormancy in production is mainly concentrated sulfuric acid corrosion seed coat, but denseSulphuric acid is a strong corrosive, and operation has certain danger; The more difficult assurance of etching time, the long acid evil that easily causes of etching time; DenseSulfuric acid price is high, and difficult recovery, easily causes environmental pollution. The method that solves seed quality inequality is mainly classification again after cleaning, pointThe technology of level is mainly by screening by the seed of different sizes separately. Abrus cantoniensis Hance seed coat more firmly, thinner, use the concentrated sulfuric acidCorrosion seed coat is difficult to take the time, and differing maturity seed size is close, and screening cannot be separated. Therefore, need a kind of low one-tenth badlyOriginally, solve abrus cantoniensis Hance seed dormancy without dangerous, technology simple to operate, promotion seed germination.
Summary of the invention
The object of the present invention is to provide a kind of method that promotes abrus cantoniensis Hance seed germination, by seed is carried out to waterCause, seed is fully absorbed water and remove the dormancy of seed, improve percentage of seedgermination;
Another object of the present invention is after seed is plucked, to ted after-ripening, screening and wet concentration etc., guarantee kindThe uniformity of sub-maturity and plumpness.
A further object of the invention, by gathering, tedding, after-ripening, screening and wet concentration, seed water cause, seed disinfectionThe overall process of cultivating with germination effectively integrates, and improves as much as possible the germination percentage of abrus cantoniensis Hance seed.
Technical scheme provided by the invention is:
A method that promotes abrus cantoniensis Hance seed germination, is characterized in that, comprising:
Choose the abrus cantoniensis Hance seed of full maturation, cause and seed is fully absorbed water and remove described Guangzhou phase by waterThink, after the dormancy of sub-seed, at 24-26 DEG C, to replace illumination cultivation 9-11d, to described abrus cantoniensis Hance seed germination;
Wherein said seed water initiating method is:
Soak described abrus cantoniensis Hance seed 18～24 hours with 20～30% polyglycol solutions, solution temperature remains on30～40 DEG C, to soak rear separation surface seed and sunk to the bottom seed, collection sinks to the bottom seed and obtains water Priming Seeds.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, before seed is carried out to water initiationCarry out nature and ted and screen, method is as follows: pluck eight points of full above ripe fruit pods,, ted 5-7 under certain conditionThe fruit pod that it obtains being dried, beats and makes it natural cracking the dried fruit pod of gained, and then screening and removing impurities obtains Guangzhou phaseThink sub-seed.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, the choosing method of full seed is: willThe abrus cantoniensis Hance seed of gained mixes with clear water, after fully stirring, the seed of the seed sinking to the bottom and surface is separated, and obtainsFull seed;
The full seed of gained is dried to thousand of 16-19g/, is dry abrus cantoniensis Hance seed.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described abrus cantoniensis Hance is being carried outBefore illumination cultivation, carry out seed disinfection, method is as follows: gained water Priming Seeds is mixed with thimerosal and soaks certain hour, disappearPoison is rear with 3～5 seeds that are sterilized of distilled water flushing; Wherein, described thimerosal is that mass fraction is the height of 0.05-0.15%Potassium manganate solution, soak time is 1.5-2.5 hour; Or mass fraction be 0.5-1.5% liquor natrii hypochloritis, soak timeIt is 30 minutes.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described eight points of full above maturationsFruit pod is annual mid or late October, falls the cervine fruit pod collecting for one or two week before and after frost.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described in the suitable condition that teds be: willDescribed fruit pod is tedded 15-30 DEG C of room temperature ventilation, its airing thickness 1-5cm, relative air humidity 30～50%.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described dried fruit pod at least carries out 1 timeOn screening and removing impurities.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described abrus cantoniensis Hance seed is dryDryly be pitchy to smooth surface, its strophiole is wax yellow and obtains described dry jequirity seed.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, in the process that described water causes, described inPolyethylene glycol consumption is as the criterion with seed described in complete submergence, and frequently described solution is stirred in the process of soaking, straightExtremely described solution is pitchy.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described illumination cultivation condition for: by instituteObtain disinfection seed and be placed in the culture dish that germinating bed is 0.5-2% agar, intensity of illumination is 2500-3500LX, illumination in 12 hours/Dark alternately illumination in 12 hours, taking 24 hours as one-period Continuous Cultivation.
The present invention has following beneficial effect: first the present invention is directed to the feature of abrus cantoniensis Hance seed, utilize poly-second twoAlcoholic solution carries out water and causes the strong dormancy of releasing abrus cantoniensis Hance seed, utilizes steps such as tedding after-ripening, screening and wet concentration to ensureKernel maturing, plumpness and the uniformity, utilize thimerosal to kill the surface of the seed germ, and suitable germination light warm water bar is providedPart, each step is combined closely, and effectively configuration, has promoted abrus cantoniensis Hance seed germination.
Secondly, the present invention utilizes polyglycol solution to replace concentrated sulfuric acid solution to process abrus cantoniensis Hance seed, hasEffect is removed the constraint of seed coat to embryonic development, abolishes the strong dormancy of seed, and ensures that seed fully absorbs water, and improves percentage of seedgermination,The acid evil easily causing with the concentrated sulfuric acid is compared with environmental pollution, and polyglycol solution is little and pollution-free to seed damage.
Again, the present invention adopts the step of tedding after-ripening, and in the time of 5-7 days, seed is natural drying on one side, becomes on one sideRipe, both saved time, effectively guarantee again the maturity of seed, avoid the germination percentage that causes because kernel maturing is inconsistent irregularUneven; Meanwhile, the present invention utilizes screening and removing impurities and wet concentration to ensure the clean and saturation degree of seed.
Finally, compare commonsense method cultivation abrus cantoniensis Hance seed and only have 2% germinating energy and 20% germination percentage, institute of the present inventionThe method of stating is brought up to more than 9% abrus cantoniensis Hance potentiality of seed, and germination percentage is brought up to more than 75%. The present invention is mainly used inPromote abrus cantoniensis Hance seed germination, the seed seedling-raising to abrus cantoniensis Hance, large-scale planting have very high practical application valencyValue.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is elaborated, to make those of ordinary skill in the art consult after this descriptionCan implement according to this.
Promote a method for abrus cantoniensis Hance seed germination, wherein, comprising:
The collection of step 1, seed: in annual mid or late October, when abrus cantoniensis Hance fruit pod during by green flavescence seed becomeRipe, pluck eight points of full above fawn fruit pods, eight points of full above fawn fruit pods can ensure that seed is rearIn continuous natural After-ripening, reach full maturity.
Step 2, seed drying and sorting: by the fruit pod of gained after gathering, be 30～50% in room temperature, relative humidityUnder condition, natural airing 5～7 days is beaten and is made it season crack with rod after fruit pod is dry, and the field trashes such as hulling skin of winnowing with a dustpan obtain phaseThink sub-seed, then gained seed is all fallen and fully stirred in clear water, the seed of the seed sinking to the bottom and surface is separated, receiveCollection sinks to the bottom seed and obtains full good seed, good gained seed packet is installed on to aeration-drying indoor, mass of 1000 kernel after being driedApproximately 18 grams, be dry jequirity seed. The sorting of this step has comprised screening and wet concentration, and screening can ensure the clean of seedDegree, wet concentration can ensure the plumpness of seed.
Step 3, seed water cause: the dry jequirity kind of soaking gained with 20～30% polyethylene glycol (PEG) solutionSon 18～24 hours, water temperature remains on 30～40 DEG C, again separates the poor seed emerging after having soaked seed, and remaining isWater Priming Seeds. Wherein, about 18 hours, be best soak time, the time is less than 18 hours or is greater than 18 hours, itsBud rate all decreases, and 30-40 DEG C is best soaking temperature, and when water temperature rises to 80 DEG C, germination percentage can occur largelyDecline.
Step 4, seed disinfection: it is that 0.1% liquor potassic permanganate soaks that water Priming Seeds is put into mass fractionBubble sterilization, disinfecting time is 2 hours; Or the liquor natrii hypochloritis's soaking disinfection that is 1% with mass fraction 30 minutes, after sterilization, useDistilled water flushing 3～5 times. Can prevent that to seed disinfection seed from going mouldy.
Step 5, germination: the abrus cantoniensis Hance seed of disinfecting through step 4 is placed in to the culture dish that diameter is 9cmIn, in illumination box, cultivate 10 days.
The suitable time of described seed collection is before and after the Frost's Descent one or two week, i.e. 18-October 26 October, the one-tenth of seedRipe degree is higher, and its germination percentage is higher, and abrus cantoniensis Hance percentage of seedgermination and kernel maturing are closely related, and the fruit pod of collection is eightDivide full above cervine fruit pod.
The suitable condition of described seed drying is the fruit pod collecting to be placed in to room temperature ventilation dry, and allows incompleteFull seed is natural after-ripening in fruit pod, fruit pod spreading for cooling thickness < 5cm, and indoor air relative humidity 30～50%, when spreadingBetween 5～7 days, seed natural after-ripening in fruit pod can ensure the maturity of seed, ensures the uniformity of seed, avoids seed to becomeThe inhomogeneous impact on percentage of seedgermination of ripe degree, its germination percentage of the seed of natural after-ripening is higher more than 50% than common seed.
It is that first club knocks fruit pod is split that described seed cleans method, then wet concentration after the field trashes such as hulling skin of winnowing with a dustpan is cleanThe surface of the seed is smooth, pitchy, strophiole wax yellow, mass of 1000 kernel 16～19g.
The condition that described seed water causes is: the concentration of polyglycol solution is 20～30%, and the water yield is with complete submergenceSeed is degree, and the water temperature of immersion maintains 30～40 DEG C, and soak time is 18～24 hours, in immersion process, should frequently stir,Immersion finishes rear clear water color blackening brown.
Described seed suitable conditions for germination is: 25 DEG C of constant temperature, intensities of illumination are 30001x, and within 12 hours, illumination/12 are littleTime dark alternately illumination, germinating bed be 1% agar, using 24 hours as a cycle period, Continuous Cultivation 10 days.
Embodiment 1
Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and material, asWithout specified otherwise, all can obtain from commercial channels.
One, pod is plucked: respectively at plucking 4 parts of abrus cantoniensis Hance (Abrus in October, 2013～NovemberCantoniensisHance) eight points of full above cervine mellow fruit pods, the acquisition time of every part of seed, placeRefer to table 1.
Table 1 abrus cantoniensis Hance seed collection record
Two, seed drying and sorting: respectively by the pod collecting, the condition that is 30～50% in room temperature, relative humidityUnder, natural airing 1 week, treats that fruit pod is dry, when the whole flavescence look of fruit pod shell, opens with hand breakaway, and the field trashes such as hulling skin of winnowing with a dustpan, thenSeed is all poured in clear water and is fully stirred, the seed of the seed sinking to the bottom and surface is separated to the seed dustpan sinking to the bottomBe installed on aeration-drying Indoor Natural dry. The gross weight that records 4 parts of dry jequirity seeds after dry is respectively 2.5 thousandGram, 5.6 kilograms, 3.1 kilograms, 3.8 kilograms, mass of 1000 kernel is respectively 17.28 grams, 16.81 grams, 17.45 grams, 18.07 grams.
Three, seed water causes: 500～1000, the dry jequirity seed of 4 parts of separate sources is soaked in respectivelyIn 30%PEG solution, what water caused refers to table 2 with kind a source, sowing quantity, the water yield, water temperature, seed soaking time. With the kind of not soaking seedSub-tested number 1 compares.
Table 2 abrus cantoniensis Hance water causes record
Four, seed disinfection: it is that 0.1% liquor potassic permanganate soaks and disappears that water Priming Seeds is put into mass fractionPoison, disinfecting time is 2 hours, uses distilled water flushing 3～5 times after sterilization. Control treatment seed-test number 1 is not disinfected.
Five, germinate: the abrus cantoniensis Hance seed after potassium permanganate sterilization is placed in to 1% in the culture dish that diameter is 9cmOn agar germinating bed, be 30001x in 25 DEG C of constant temperature, intensity of illumination, the 12 hours illumination/12 hour dark alternately light of illuminationAccording to cultivating in incubator 10 days. Each culture dish is put 25 seeds, and each processing repeats 8 times, the germination of control treatmentCondition is the same. Pendulum kind of latter the 5th day potentiality of seed, the 10th day survey percentage of seedgermination, statistics is in table 3. From table 3, logicalCross sprouting and seedling that the technology of the present invention can promote abrus cantoniensis Hance seed effectively, with the germinating energy 2.0% of control treatment, send outBud rate 20.0% is compared, and the abrus cantoniensis Hance potentiality of seed of processing 3 brings up to 9.0%, and germination percentage has brought up to 75.0%.
Table 3 abrus cantoniensis Hance germination statistics
Although embodiment of the present invention are open as above, it is not restricted in description and embodiment listedUse, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easilyRealize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention does not limitIn specific details.
Claims (10)
1. a method that promotes abrus cantoniensis Hance seed germination, is characterized in that, comprising:
Choose the abrus cantoniensis Hance seed of full maturation, cause and seed is fully absorbed water and remove described abrus cantoniensis Hance by waterAfter the dormancy of seed, at 24-26 DEG C, replace illumination cultivation 9-11d, to described abrus cantoniensis Hance seed germination;
Wherein said seed water initiating method is:
Soaking described abrus cantoniensis Hance seed 18～24 hours with 20～30% polyglycol solutions, solution temperature remains on 30～40 DEG C, to soak rear separation surface seed and sunk to the bottom seed, collection sinks to the bottom seed and obtains water Priming Seeds.
2. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 1, is characterized in that, seed is being drawnBefore sending out, carry out nature and ted and screen, method is as follows: pluck eight points of full above ripe fruit pods, under certain condition,Ted the fruit pod that obtains being dried for 5-7 days, the dried fruit pod of gained is beaten and makes it natural cracking, then screening and removing impurities obtainsAbrus cantoniensis Hance seed.
3. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 2, is characterized in that, the choosing of full seedMethod is: the abrus cantoniensis Hance seed of gained is mixed with clear water, after fully stirring, the seed of the seed sinking to the bottom and surface is dividedFrom, obtain full seed;
The full seed of gained is dried to thousand of 16-19g/, is dry jequirity seed.
4. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 1, is characterized in that, to described Guangzhou phaseThink son and carry out carrying out seed disinfection before illumination cultivation, method is as follows: gained water Priming Seeds is mixed with thimerosal and soaks oneFix time, sterilization is rear with 3～5 seeds that are sterilized of distilled water flushing; Wherein, described thimerosal is that mass fraction is 0.05-0.15% liquor potassic permanganate, soak time is 1.5-2.5 hour; Or the clorox that mass fraction is 0.5-1.5% is moltenLiquid, soak time is 30 minutes.
5. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 2, is characterized in that, described eight points full withOn mellow fruit pod be annual mid or late October, the cervine fruit pod collecting for one or two week before and after the Frost's Descent.
6. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 2, is characterized in that, described in the condition of teddingBe: described fruit pod is tedded to its airing thickness 1-5cm, relative air humidity 30～50% 15-30 DEG C of room temperature ventilation.
7. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 2, is characterized in that, described dried fruit pod extremelyCarry out less the screening and removing impurities on 1 time.
8. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 3, is characterized in that, by described Guangzhou yearning between loversSub-seed drying to smooth surface is pitchy, and its strophiole is wax yellow and obtains described dry abrus cantoniensis Hance seed.
9. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 1, is characterized in that, the mistake that described water causesCheng Zhong, described polyethylene glycol consumption is as the criterion with seed described in complete submergence, and in the process of soaking, described solution is stirredMix, until described solution is pitchy.
10. the method for promotion abrus cantoniensis Hance seed germination as claimed in claim 4, is characterized in that, described illumination cultivationCondition is: gained disinfection seed is placed in to the culture dish that germinating bed is 0.5-2% agar, and intensity of illumination is 2500-3500LX,Illumination/12 hour dark alternately illumination in 12 hours, taking 24 hours as one-period Continuous Cultivation.
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| CN107646241A (en) * | 2017-11-12 | 2018-02-02 | 陈培党 | A kind of plantation process for improving Canton love-pea vine breeding germination percentage |
| CN114467407B (en) * | 2022-01-04 | 2023-06-13 | 海南大学 | Method for economically and rapidly breaking dormancy of acacia confusa seeds and promoting germination of acacia confusa seeds |
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| CN103004326A (en) * | 2012-12-23 | 2013-04-03 | 昆明学院 | Initiation method for promoting germination of seed of Melasma arvense (Benth.) Hand.-Mazz. |
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| CN103004326A (en) * | 2012-12-23 | 2013-04-03 | 昆明学院 | Initiation method for promoting germination of seed of Melasma arvense (Benth.) Hand.-Mazz. |
Non-Patent Citations (3)
| Title |
|---|
| PEG 浸种对老化的蔓性千斤拔种子萌发及活力的影响;冯世鑫等;《作物杂志》;20131231(第1期);第136-139页 * |
| 毛相思子种子发芽试验研究;董青松等;《种子》;20100430;第29卷(第4期);第12-15页 * |
| 聚乙烯醇渗调对台湾相思树种子活力的影响;黎盛隆;《华南农业大学学报(自然科学版)》;19921231;第13卷(第1期);第87-92页 * |
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