CN104365219A - Method of accelerating germination of seeds of Abrus cantoniensis - Google Patents
Method of accelerating germination of seeds of Abrus cantoniensis Download PDFInfo
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- CN104365219A CN104365219A CN201410750670.5A CN201410750670A CN104365219A CN 104365219 A CN104365219 A CN 104365219A CN 201410750670 A CN201410750670 A CN 201410750670A CN 104365219 A CN104365219 A CN 104365219A
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- cantoniensis hance
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Abstract
The invention discloses a method of accelerating germination of seeds of Abrus cantoniensis. The method includes: collecting seeds, drying and sorting the seeds, hydro-priming the seeds, and sterilizing and germinating the seeds. According to the features of the seeds of Abrus cantoniensis, polyethylene glycol solution is used in hydro-priming to remove dormancy of hard seeds of Abrus cantoniensis, the steps such as tedding and after-ripening, screening and water separation are utilized to ensure seed maturity, fullness and uniformity, sterilizing liquid is used to kill germs on the surfaces of the seeds, moderate light, temperature and water conditions for germination are provided, and germination of the seeds of Abrus cantoniensis is accelerated. Compared with the common cultivation methods of seeds of Abrus cantoniensis in which germination potential is 2% and germination rate is 20%, the method has the advantages that the germination potential is increased to greater than 9%, and the germination rate is increased to greater than 75%.
Description
Technical field
The present invention relates to plant seed termination of diapause technical field, particularly a kind of method promoting abrus cantoniensis Hance seed germination.
Technical background
Abrus cantoniensis Hance (Abrus cantoniensis Hance) is pulse family Abrus climbing shrub, root, complete stool and seed all can hyoscines, medicinal material name " Canton love-pea vine " during all herbal medicine, can clearing heat and promoting diuresis, Shugan Zhitong, for the treatment of acute, chronic hepatitis and mastitis.Along with the successful exploitation listing of the multiple Chinese patent drug product such as " Herba abri capsule ", " Herba Abri pill ", " Guanule contg. ' Jigucao particle ", " Guanule contg. ' Jigucao electuary ", the demand of market to Canton love-pea vine medicinal material constantly increases, and artificial cultivation abrus cantoniensis Hance is the crucial way of the contradiction between the expansion of solution market demand and wild resource amount die-off.
Abrus cantoniensis Hance seed is tame main propagating materials, but seed permeating properties of seed capsule difference and the machinery restriction had embryonic development, cause percentage of seedgermination low, namely there is strong dormancy, seed difficulty is sprouted has become its serious problems expanding cultivation and effectively utilize further of restriction.In addition, the florescence of abrus cantoniensis Hance is longer, the fruit phase is longer, the fruit pod maturity causing same batch to gather is inconsistent, there is significant difference in the quality index such as moisture, weight, plumpness, appearance color, germination rate of differing maturity seed, seed quality is uneven emerges when being also and causing artificial cultivation uneven major reason.
At present, production is removed the method mainly concentrated sulfuric acid corrosion seed coat of strong Seeds of Medicinal Plants dormancy, but the concentrated sulfuric acid has severe corrosive, operation has certain danger; The more difficult assurance of etching time, etching time is long easily causes acid evil; Concentrated sulfuric acid price is high, and difficult recovery, easily causes environmental pollution.Classification again after the method solving seed quality inequality mainly cleans, the technology of classification mainly by screening by the seed of different size separately.Abrus cantoniensis Hance seed coat more firmly, thinner, use the concentrated sulfuric acid corrosion seed coat be difficult to take the time, differing maturity seed size is close, and screening cannot be separated.Therefore, need a kind of low cost badly, solve abrus cantoniensis Hance seed dormancy without dangerous, technology simple to operate, promote seed germination.
Summary of the invention
The object of the present invention is to provide a kind of method promoting abrus cantoniensis Hance seed germination, by carrying out water initiation to seed, seed fully being absorbed water and removes the dormancy of seed, improve percentage of seedgermination;
Another object of the present invention is, after plucking seed, ted after-ripening, screening and wet concentration etc., ensures the uniformity of kernel maturing and plumpness.
A further object of the invention, by gathering, ted after-ripening, screening and wet concentration, seed water cause, seed disinfection and seed sprouting are cultivated overall process effectively integrates, and improves the germination rate of abrus cantoniensis Hance seed as much as possible.
Technical scheme provided by the invention is:
Promote a method for abrus cantoniensis Hance seed germination, it is characterized in that, comprising:
Choose the abrus cantoniensis Hance seed of full maturation, caused seed is fully absorbed water and after removing the dormancy of described abrus cantoniensis Hance seed, at 24-26 DEG C, carries out alternate illumination cultivate 9-11d, to described abrus cantoniensis Hance seed germination by water;
Wherein said seed water initiating method is:
Soak described abrus cantoniensis Hance seed 18 ~ 24 hours with 20 ~ 30% polyglycol solutions, solution temperature remains on 30 ~ 40 DEG C, has soaked rear separation surface seed and has sunk to the bottom seed, and collection sinks to the bottom seed and namely obtains water Priming Seeds.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, before carrying out water initiation to seed, carry out nature ted and screen, method is as follows: the fruit pod of plucking eight points of full above maturations, under certain condition, ted and within 5-7 days, obtain dry fruit pod, beat the dried fruit pod of gained and make it natural cracking, then screening and removing impurities obtains abrus cantoniensis Hance seed.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, the choosing method of full seed is: mixed with clear water by the abrus cantoniensis Hance seed of gained, after fully stirring, is separated by the seed sunk to the bottom, namely obtains full seed with the seed of surface;
The full seed of gained is dried to 16-19g/ thousand, is dry abrus cantoniensis Hance seed.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, seed disinfection was carried out before illumination cultivation is carried out to described abrus cantoniensis Hance, method is as follows: mixed with thimerosal by gained water Priming Seeds and soak certain hour, with distilled water flushing 3 ~ 5 seeds that are sterilized after sterilization; Wherein, the liquor potassic permanganate of described thimerosal to be mass fraction be 0.05-0.15%, soak time is 1.5-2.5 hour; Or mass fraction is the liquor natrii hypochloritis of 0.5-1.5%, soak time is 30 minutes.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described eight points of full above mellow fruit pods are annual mid or late October, fall the cervine fruit pod collected for one or two week before and after frost.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described in the suitable condition that teds be: described fruit pod is tedded 15-30 DEG C of room temperature ventilation, its airing thickness 1-5cm, relative air humidity 30 ~ 50%.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described dried fruit pod at least carries out the screening and removing impurities on 1 time.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, be pitchy by described abrus cantoniensis Hance seed drying to smooth surface, its strophiole is the jequirity seed that namely wax yellow obtains described drying.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, in the process that described water causes, described polyethylene glycol consumption is as the criterion with seed described in complete submergence, and frequently described solution is stirred, until described solution is pitchy in the process of soaking.
Preferably, in the method for described promotion abrus cantoniensis Hance seed germination, described illumination cultivation condition is: gained disinfection seed is placed in the culture dish that germinating bed is 0.5-2% agar, intensity of illumination is 2500-3500LX, the alternate illumination of 12 h light/12 h dark was one-period continuous culture with 24 hours.
The present invention has following beneficial effect: the feature that first the present invention is directed to abrus cantoniensis Hance seed, utilize polyglycol solution to carry out water to cause the strong dormancy of releasing abrus cantoniensis Hance seed, utilize steps such as tedding after-ripening, screening and wet concentration to ensure kernel maturing, plumpness and the uniformity, thimerosal is utilized to kill the surface of the seed germ, and suitable germination light warm water condition is provided, each step is combined closely, effective configuration, facilitates abrus cantoniensis Hance seed germination.
Secondly, the present invention utilizes polyglycol solution to replace concentrated sulfuric acid solution to process abrus cantoniensis Hance seed, efficient solution is except seed coat is to the constraint of embryonic development, abolish the strong dormancy of seed, and ensure that seed fully absorbs water, improve percentage of seedgermination, the acid easily caused with concentrated sulfuric acid evil is compared with environmental pollution, and polyglycol solution is little and pollution-free to seed damage.
Again, the present invention adopts and teds the step of after-ripening, in the time of 5-7 days, and seed natural seasoning, maturation, both saved time, effectively guaranteed again the maturity of seed on one side, avoided the germination rate that causes because kernel maturing is inconsistent uneven; Meanwhile, the present invention utilizes screening and removing impurities and wet concentration to ensure that the clean of seed and degree of saturation.
Finally, compare commonsense method cultivation abrus cantoniensis Hance seed and only have 2% germination vigor and 20% germination rate, the method for the invention makes abrus cantoniensis Hance potentiality of seed bring up to more than 9%, and germination rate brings up to more than 75%.The present invention is mainly used in promoting abrus cantoniensis Hance seed germination, is of very high actual application value to the seed seedling-raising of abrus cantoniensis Hance, large-scale planting.
Embodiment
Below in conjunction with embodiment, the present invention is elaborated, can implement according to this after consulting this specification to make those of ordinary skill in the art.
Promote a method for abrus cantoniensis Hance seed germination, wherein, comprising:
The collection of step one, seed: in annual mid or late October, when abrus cantoniensis Hance fruit pod is by seed maturity during green flavescence, pluck eight points of full above fawn fruit pods, eight points of full above fawn fruit pods can ensure that seed reaches full maturity in follow-up natural After-ripening.
Step 2, seed drying and sorting: will the fruit pod of rear gained be gathered, under room temperature, relative moisture are the condition of 30 ~ 50%, nature airing 5 ~ 7 days, beat with rod after the drying of fruit pod and make it season crack, the field trashes such as hulling skin of winnowing with a dustpan obtain jequirity seed, again gained seed is all fully stirred in clear water, the seed of the seed sunk to the bottom and surface is separated, collection sinks to the bottom seed and obtains full good seed, good for gained seed packet is installed on aeration-drying indoor, thousand kernel weight about 18 grams after drying, is dry jequirity seed.The sorting of this step includes screening and wet concentration, and screening can ensure the clean degree of seed, and wet concentration can ensure the plumpness of seed.
Step 3, seed water cause: with the jequirity seed 18 ~ 24 hours of the drying of 20 ~ 30% polyethylene glycol (PEG) solution immersion gained, water temperature remains on 30 ~ 40 DEG C, again be separated the poor seed emerged after having soaked seed, remaining is water Priming Seeds.Wherein, 18 hours are best soak times, and the time is less than 18 hours or is greater than 18 hours, and its germination rate all decreases, and 30-40 DEG C is best soaking temperature, and when water temperature rises to 80 DEG C, germination rate can occur to decline largely.
Step 4, seed disinfection: water Priming Seeds is put into mass fraction be 0.1% liquor potassic permanganate carry out soaking disinfection, disinfecting time is 2 hours; Or be liquor natrii hypochloritis's soaking disinfection 30 minutes of 1% with mass fraction, with distilled water flushing 3 ~ 5 times after sterilization.Can prevent seed from going mouldy to seed disinfection.
Step 5, germination: the abrus cantoniensis Hance seed of disinfecting through step 4 is placed in the culture dish that diameter is 9cm, cultivate 10 days in illumination box.
The suitable time of described seed collection is before and after the Frost's Descent one or two week, i.e. 18-October 26 October, the maturity of seed is higher, and its germination rate is higher, abrus cantoniensis Hance percentage of seedgermination and kernel maturing closely related, the fruit pod of collection is eight points of full above cervine fruit pods.
The suitable condition of described seed drying the fruit pod collected is placed in room temperature ventilation dry, allow the natural after-ripening in fruit pod of completely not full seed, fruit pod spreading for cooling thickness < 5cm, indoor air relative humidity 30 ~ 50%, spread 5 ~ 7 days time, seed natural after-ripening in fruit pod can ensure the maturity of seed, ensure the uniformity of seed, avoid the uneven impact on percentage of seedgermination of kernel maturing, its germination rate of seed of natural after-ripening is higher than common seed by more than 50%.
It is that first club knocks fruit pod is split that described seed cleans method, then wet concentration after the field trashes such as hulling skin of winnowing with a dustpan, clean the surface of the seed is smooth, pitchy, strophiole wax yellow, thousand kernel weight 16 ~ 19g.
The condition that described seed water causes is: the concentration of polyglycol solution is 20 ~ 30%, the water yield is with complete submergence seed for degree, and the water temperature of immersion maintains 30 ~ 40 DEG C, and soak time is 18 ~ 24 hours, frequently should stir in immersion process, soak and terminate rear clear water color blackening brown.
Described seed suitable conditions for germination is: 25 DEG C of constant temperature, intensities of illumination are 30001x, and the alternate illumination of 12 h light/12 h dark, germinating bed are 1% agar, using 24 hours as a cycle period, and continuous culture 10 days.
Embodiment 1
Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and material, if no special instructions, all can obtain from commercial channels.
One, pod is plucked: respectively at eight points that pluck 4 parts of abrus cantoniensis Hances (Abruscantoniensis Hance) in October, 2013 ~ November full above cervine mellow fruit pods, table 1 is referred in acquisition time, the place of every part of seed.
Table 1 abrus cantoniensis Hance seed collection record
Two, seed drying and sorting: the pod will collected respectively, under room temperature, relative moisture are the condition of 30 ~ 50%, nature airing 1 week, treat that fruit pod is dry, fruit pod shell all flavescence look time open with hand breakaway, field trashes such as hulling skin of winnowing with a dustpan, then seed is all poured in clear water fully stirs, the seed of the seed sunk to the bottom and surface is separated, and it is dry that the seed dustpan sunk to the bottom is installed on aeration-drying Indoor Natural.The gross weight recording 4 parts of dry jequirity seeds after drying is respectively 2.5 kilograms, 5.6 kilograms, 3.1 kilograms, 3.8 kilograms, and thousand kernel weight is respectively 17.28 grams, 16.81 grams, 17.45 grams, 18.07 grams.
Three, seed water causes: be soaked in 30%PEG solution respectively by 500 ~ 1000, the jequirity seed of the drying of 4 parts of separate sources, and what water caused refers to table 2 with kind of a source, sowing quantity, the water yield, water temperature, seed soaking time.Compare with the seed-test of not soaking seed number 1.
Table 2 abrus cantoniensis Hance water causes record
Four, seed disinfection: water Priming Seeds is put into mass fraction be 0.1% liquor potassic permanganate carry out soaking disinfection, disinfecting time is 2 hours, with distilled water flushing 3 ~ 5 times after sterilization.Control treatment seed-test number 1 is not disinfected.
Five, germinate: the abrus cantoniensis Hance seed after potassium permanganate sterilization is placed on 1% agar germinating bed in the culture dish that diameter is 9cm, be 30001x in 25 DEG C of constant temperature, intensity of illumination, cultivate 10 days in the illumination box of the alternate illumination of 12 h light/12 h dark.25 seeds put by each culture dish, and each process repeats 8 times, and the Germination Conditions of control treatment is the same.Pendulum kind of latter 5th day potentiality of seed, the 10th day survey percentage of seedgermination, statistics is in table 3.From table 3, effectively can be promoted sprouting and the seedling of abrus cantoniensis Hance seed by the technology of the present invention, with the germination vigor 2.0% of control treatment, germination rate 20.0% is compared, the abrus cantoniensis Hance potentiality of seed of process 3 brings up to 9.0%, and germination rate has brought up to 75.0%.
Table 3 abrus cantoniensis Hance germination is added up
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (10)
1. promote a method for abrus cantoniensis Hance seed germination, it is characterized in that, comprising:
Choose the abrus cantoniensis Hance seed of full maturation, caused seed is fully absorbed water and after removing the dormancy of described abrus cantoniensis Hance seed, at 24-26 DEG C, carries out alternate illumination cultivate 9-11d, to described abrus cantoniensis Hance seed germination by water;
Wherein said seed water initiating method is:
Soak described abrus cantoniensis Hance seed 18 ~ 24 hours with 20 ~ 30% polyglycol solutions, solution temperature remains on 30 ~ 40 DEG C, has soaked rear separation surface seed and has sunk to the bottom seed, and collection sinks to the bottom seed and namely obtains water Priming Seeds.
2. the method promoting abrus cantoniensis Hance seed germination as claimed in claim 1, it is characterized in that, before causing seed, carry out nature ted and screen, method is as follows: the fruit pod of plucking eight points of full above maturations, under certain condition, ted and within 5-7 days, obtain dry fruit pod, beat the dried fruit pod of gained and make it natural cracking, then screening and removing impurities obtains abrus cantoniensis Hance seed.
3. the method promoting abrus cantoniensis Hance seed germination as claimed in claim 2, it is characterized in that, the choosing method of full seed is: mixed with clear water by the abrus cantoniensis Hance seed of gained, after fully stirring, the seed sunk to the bottom is separated with the seed of surface, namely obtains full seed;
The full seed of gained is dried to 16-19g/ thousand, is dry jequirity seed.
4. the method promoting abrus cantoniensis Hance seed germination as claimed in claim 1, it is characterized in that, seed disinfection was carried out before illumination cultivation is carried out to described abrus cantoniensis Hance, method is as follows: mixed with thimerosal by gained water Priming Seeds and soak certain hour, with distilled water flushing 3 ~ 5 seeds that are sterilized after sterilization; Wherein, the liquor potassic permanganate of described thimerosal to be mass fraction be 0.05-0.15%, soak time is 1.5-2.5 hour; Or mass fraction is the liquor natrii hypochloritis of 0.5-1.5%, soak time is 30 minutes.
5. the method promoting abrus cantoniensis Hance seed germination as claimed in claim 2, is characterized in that, described eight points of full above mellow fruit pods are annual mid or late October, the cervine fruit pod collected for before and after the Frost's Descent one or two week.
6. the as claimed in claim 2 method promoting abrus cantoniensis Hance seed germination, is characterized in that, described in the condition of tedding be: described fruit pod is tedded 15-30 DEG C of room temperature ventilation, its airing thickness 1-5cm, relative air humidity 30 ~ 50%.
7. the method promoting abrus cantoniensis Hance seed germination as claimed in claim 2, it is characterized in that, described dried fruit pod at least carries out the screening and removing impurities on 1 time.
8. the as claimed in claim 3 method promoting abrus cantoniensis Hance seed germination, is characterized in that, be pitchy by described abrus cantoniensis Hance seed drying to smooth surface, its strophiole is the abrus cantoniensis Hance seed that namely wax yellow obtains described drying.
9. the method promoting abrus cantoniensis Hance seed germination as claimed in claim 1, it is characterized in that, in the process that described water causes, described polyethylene glycol consumption is as the criterion with seed described in complete submergence, and described solution is stirred, until described solution is pitchy in the process of soaking.
10. the method promoting abrus cantoniensis Hance seed germination as claimed in claim 1, it is characterized in that, described illumination cultivation condition is: gained disinfection seed is placed in the culture dish that germinating bed is 0.5-2% agar, intensity of illumination is 2500-3500LX, the alternate illumination of 12 h light/12 h dark was one-period continuous culture with 24 hours.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106211855A (en) * | 2016-07-27 | 2016-12-14 | 惠州市黑娜尔科技有限公司 | The sowing pre-treating method of Semen Phaseoli seed |
| CN107646241A (en) * | 2017-11-12 | 2018-02-02 | 陈培党 | A kind of plantation process for improving Canton love-pea vine breeding germination percentage |
| CN114467407A (en) * | 2022-01-04 | 2022-05-13 | 海南大学 | Method for economically and rapidly breaking dormancy of acacia longissima seeds and promoting germination of acacia longissima seeds |
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| CN103004326A (en) * | 2012-12-23 | 2013-04-03 | 昆明学院 | Initiation method for promoting germination of seed of Melasma arvense (Benth.) Hand.-Mazz. |
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| CN103004326A (en) * | 2012-12-23 | 2013-04-03 | 昆明学院 | Initiation method for promoting germination of seed of Melasma arvense (Benth.) Hand.-Mazz. |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211855A (en) * | 2016-07-27 | 2016-12-14 | 惠州市黑娜尔科技有限公司 | The sowing pre-treating method of Semen Phaseoli seed |
| CN107646241A (en) * | 2017-11-12 | 2018-02-02 | 陈培党 | A kind of plantation process for improving Canton love-pea vine breeding germination percentage |
| CN114467407A (en) * | 2022-01-04 | 2022-05-13 | 海南大学 | Method for economically and rapidly breaking dormancy of acacia longissima seeds and promoting germination of acacia longissima seeds |
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