CN102524194B - Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests - Google Patents
Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests Download PDFInfo
- Publication number
- CN102524194B CN102524194B CN201210043792.1A CN201210043792A CN102524194B CN 102524194 B CN102524194 B CN 102524194B CN 201210043792 A CN201210043792 A CN 201210043792A CN 102524194 B CN102524194 B CN 102524194B
- Authority
- CN
- China
- Prior art keywords
- bark beetle
- mite
- bark
- pyemotes
- beetle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a method for artificially propagating pyemotes bark beetle and the application in biological control of bark beetle pests. The method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis comprises the steps as follows: firstly, a host that is Callosobruchus chinensis is produced; secondly, protospecies of pyemotes bark beetle is prepared; and thirdly, pyemotes bark beetle is propagated. The method and the application have the benefits as follows: the difficult problem of propagating pyemotes bark beetle to replace the host is solved; pyemotes bark beetle can be propagated in large quantities; the method is simple and convenient to operate; large-scale production can be carried out; the method is applicable to control Scolytus seulensis Murayama pests on forests and fruit trees such as apricot trees, peach trees and plum trees; the problem that the forests and the fruit trees such as the apricot trees, the peach trees and the plum trees are damaged by the Scolytus seulensis Murayama pests and whole branches or entire trees die back further because the parasitism rate of pyemotes bark beetle is lower can be solved; therefore, the requirements of forestry and fruit Industry production can be satisfied, pollution on the environment cannot be caused, and the method is an effective technological means for controlling the Scolytus seulensis Murayama pests in an environmental protection manner.
Description
Technical field
the present invention relates to the method for mass rearing bark beetle Pu mite and in the technical field of bark beetle biological control of insect pests, be method and the application in bark beetle biological control of insect pests of a kind of mass rearing bark beetle Pu mite.
Background technology
crinosity bark beetle (
scolytus seulensismurayama) belong to Scolytidae, in the equal scope in Xinjiang, all there is generation, with Aksu, Kuerle, Luntai County, Kuqa County one band, occur comparatively serious, the branches of fruit trees such as main harm apricot, peach, Lee, cause phloem transport tissue damage, hinder the transportation of moisture and nutriment, finally cause training or complete stool withered withered.Cause tree body to dredge tissue die.
< < Xinjiang agricultural science and technology > >, 1993, (5): in the 215th page to the 216th page, by medium " generation of the little stupid worm of crinosity and the integrated control technique " literary composition delivered of Lee, made following report: the mid-80 crinosity bark beetle is in the storehouse of Aksu, Xinjiang car, Shaya, newly and just have generation, at storehouse car to 0.25 * 10
4
hm
2
be injured serious Xing garden investigation, on average has worm strain rate to reach 17.5%, and withered rate reaches 9.23%, and endangering serious apricot garden has worm strain rate up to 88% to 100%, and withered rate reaches 52% to 81%.
< < plant protection technology with promote > > 1995(2): following report made in " generation and the study on prevention of apricot crinosity bark beetle " literary composition of being delivered by Zhao Linzhong etc. in the 11st page to the 12nd page: the strain rate of being injured in 1991-1999 Nian Xing gardens, Hami Prefecture reaches 8.2% to 42.6%, and production loss rate is up to 64.96% to 78.14%.
< < plant protection > > 2009; following report made in " Kaxgar Prefecture crinosity bark beetle Infection and damage rule " literary composition of being delivered by Li Hong etc. in the 35 (6): the 135th page to the 138th page: Kaxgar Prefecture is caused harm in lethal apricot less than 1%, 2008 Nian Xing garden by crinosity bark beetle 2005 to cause harm up to 25.9%.Since two thousand two, crinosity bark beetle generation area and the extent of injury constantly increase in Luntai County.Whole county apricot bark beetle generation area in 2007 is up to 4.5 ten thousand mu according to statistics, and wherein 20 years Sheng Laoxing garden hazard ratios are up to 80%, has caused more than ten ten thousand strain (branch) apricots withered, and the healthy and sustainable development of whole county apricot industry has been formed to great threat.
at present, the main method of preventing and treating crinosity bark beetle insect has two kinds, and a kind of is to kill insect with chemical insecticide, because crinosity bark beetle is to bore moth harm, has increased difficulty therefore to effectively control; Another kind is to utilize the natural enemy of insect to eliminate destructive insects, at natural world, found that the natural enemy of parasitic crinosity bark beetle is bark beetle Pu mite, this mite nature parasitic rate is 10% to 30%, but this far can not stop the breeding of crinosity bark beetle insect, thereby cause training or complete stool withered dead, have a strong impact on the development of the fruit-bearing forest industry such as apricot, peach, Lee, also affected expanding economy.
due to crinosity bark beetle, the navel abdomen bark beetle larva raising difficulty of getting up comparatively, and the natural parasitic rate of bark beetle Pu mite is lower again, so amount reproduction bark beetle Pu mite is more difficult, thus also bark beetle Pu acarid control crinosity bark beetle for impact.
Summary of the invention
the invention provides a kind of method with adzuki bean weevil mass rearing bark beetle Pu mite, overcome the deficiency of prior art, solved can not amount reproduction bark beetle Pu mite problem.
the present invention also provides a kind of application in bark beetle biological control of insect pests with adzuki bean weevil mass rearing bark beetle Pu mite, overcome the deficiency of prior art, solved bark beetle Pu mite parasitic rate compared with low and cause the fruit trees such as apricot, peach, Lee to be made its training or the withered dead problem of complete stool by crinosity bark beetle pest damage.
one of technical scheme of the present invention obtains in the following manner: a kind of method with adzuki bean weevil mass rearing bark beetle Pu mite, and it carries out in the steps below:
first step Hosts adzuki bean weevil produces: the access of adzuki bean weevil adult is filled in the crisper of mung bean or cowpea, until beans surface, produce and have after ovum, raising is 20 ℃ to 32 ℃ of temperature, under relative moisture 40% to 90% condition, at bean flour, see that larva stings into 1 circular emergence hole, plants skin and retain when transparent, transparent kind skin is needled, prepared access bark beetle Pu mite;
the preparation of second step bark beetle Pu mite original seed: the bark beetle Pu mite from field acquisition crinosity bark beetle larva or pupal cell, be placed in culture dish, in 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, to raise 6 days to 13 days, the bark beetle Pu mite that raising obtains is as original seed;
the expansion of the 3rd step bark beetle Pu mite is numerous: the mung bean that the interior transparent kind of skin that has adzuki bean weevil Larva and a pupa needled is inserted in gauze bag, with bark beetle Pu mite original seed and Hosts adzuki bean weevil larva or the access of the pupa ratio of 1: 4, the gauze bag of inoculation is inserted in the crisper with sand net, put into again 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, raise 6 days to 13 days, so just breed out a large amount of bark beetle Pu mites.
further optimization and/or the selection to one of technique scheme below:
the diameter of above-mentioned culture dish is 9 centimetres.
at above-mentioned culture dish, scribble vaseline around.
the length of above-mentioned crisper is that 30 centimetres, width are 20 centimetres, are highly 8 centimetres, the husky net of lid and both sides band.
in above-mentioned the 3rd step, the length of gauze bag is 5, width is 7 centimetres, and the addition of mung bean is 1/4th capacity of gauze bag, and gauze bag is 80 order to 100 objects.
two of technical scheme of the present invention obtains in the following manner: a kind of application in bark beetle biological control of insect pests according to the above-mentioned bark beetle Pu mite obtaining by the method for adzuki bean weevil mass rearing bark beetle Pu mite, when breed a large amount of output of bark beetle Pu mite time, gauze bag one side is tufted into apricot and has by the brill channel of bark beetle.
further optimization and/or the selection to two of technique scheme below:
the time that above-mentioned bark beetle Pu mite discharges in field, South Sinkiang, Xinjiang is: every year at the beginning of by the end of April to 6 month or by the end of August to by the end of September.
the invention has the beneficial effects as follows: the invention solves a difficult problem that expands breeding bark beetle Pu mite Hosts; realized amount reproduction bark beetle Pu mite; easy and simple to handle; can carry out large-scale production; be applied to prevent and treat apricot, peach, Li Denglin, fruit tree crinosity bark beetle insect; solve bark beetle Pu mite parasitic rate compared with low and cause apricot, peach, Li Denglin, fruit tree to be made its training or the withered dead problem of complete stool by crinosity bark beetle pest damage; thereby the needs that forests and fruit industry is produced have been met; and environment not being produced and polluted, is the effective technology means that environmental protection prevents and treats crinosity bark beetle insect.
Embodiment
the present invention is not subject to the restriction of following embodiment, can determine concrete embodiment according to the technical scheme of the invention described above and actual conditions.
below in conjunction with embodiment, the present invention is done to further discussion:
embodiment 1, and the method for this use adzuki bean weevil mass rearing bark beetle Pu mite is carried out in the steps below:
first step Hosts adzuki bean weevil produces: the access of adzuki bean weevil adult is filled in the crisper of mung bean or cowpea, until beans surface, produce and have after ovum, raising is 20 ℃ to 32 ℃ of temperature, under relative moisture 40% to 90% condition, at bean flour, see that larva stings into 1 circular emergence hole, plants skin and retain when transparent, transparent kind skin is needled, prepared access bark beetle Pu mite;
the preparation of second step bark beetle Pu mite original seed: gather the bark beetle Pu mite on crinosity bark beetle larva or pupal cell from field (generally can from having the apricot of crinosity bark beetle insect), be placed in culture dish, in 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, raise 6 days to 13 days, the bark beetle Pu mite that raising obtains is as original seed;
the expansion of the 3rd step bark beetle Pu mite is numerous: the mung bean that the interior transparent kind of skin that has adzuki bean weevil Larva and a pupa needled is inserted in 80 order to 100 object gauze bags, with bark beetle Pu mite original seed and Hosts adzuki bean weevil larva or the access of the pupa ratio of 1: 4, the gauze bag of inoculation is inserted in the crisper with sand net, put into again 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, raise 6 days to 13 days, so just breed out a large amount of bark beetle Pu mites.
embodiment 2, are with the difference of embodiment 1: the diameter of the culture dish of embodiment 2 is 9 centimetres.
embodiment 3, are with the difference of embodiment 2: the culture dish of embodiment 3 scribbles vaseline around.
embodiment 4, are with the difference of embodiment 1 to embodiment 3: the length of the crisper of embodiment 4 is that 30 centimetres, width are 20 centimetres, are highly 8 centimetres, the husky net of lid and both sides band.
embodiment 5, are with the difference of embodiment 1 to embodiment 4: in the 3rd step of embodiment 5, the length of gauze bag is 5, width is 7 centimetres, and the addition of mung bean is 1/4th capacity of gauze bag, and gauze bag is 80 order to 100 objects.
in embodiment 1 to 5, be inoculated into the bark beetle Pu mite on adzuki bean weevil larva and pupal cell, its parasitic rate has all reached 100%.
adzuki bean weevil larva can eat into the various beans of food, likes food mung bean, red bean and cowpea most, and material is easy to get; There are for 4 generations in generation to 5 in adzuki bean weevil 1 year, in as suitable in environment or storehouse, breeds, and can occur in 1 year 11 generations; Anniversary breeding, often completing for 1 generation needs 20 days to 67 days.Female Life of Adult 4 days to 25 days, male imago 5 days to 33 days.
therefore, the present invention has not only solved the problem of amount reproduction bark beetle Pu mite, and simple to operation.
embodiment 6, be with the difference of embodiment 1 to embodiment 5: embodiment 6 is the application in bark beetle biological control of insect pests by the resulting bark beetle Pu mite of embodiment 1 to embodiment 5, when breed a large amount of output of bark beetle Pu mite time, gauze bag one side is tufted into apricot and has by the brill channel of bark beetle.
embodiment 7, are with the difference of embodiment 6: the time that the bark beetle Pu mite of embodiment 7 discharges in field, South Sinkiang, Xinjiang is: every year at the beginning of by the end of April to 6 month or by the end of August to by the end of September.
embodiment 6 and embodiment 7 are applied on apricot fruit tree, and bark beetle Pu mite, at the parasitic rate average out to 45.2% of crinosity bark beetle, has greatly been improved its parasitic rate, effectively reduces the harm of crinosity bark beetle and causes the limb of apricot withered, very safe to apricot fruit.
Claims (8)
1. by a method for adzuki bean weevil mass rearing bark beetle Pu mite, it is characterized in that carrying out in the steps below:
First step Hosts adzuki bean weevil produces: the access of adzuki bean weevil adult is filled in the crisper of mung bean or cowpea, until beans surface, produce and have after ovum, raising is 20 ℃ to 32 ℃ of temperature, under relative moisture 40% to 90% condition, at bean flour, see that larva stings into 1 circular emergence hole, plants skin and retain when transparent, transparent kind skin is needled, prepared access bark beetle Pu mite;
The preparation of second step bark beetle Pu mite original seed: the bark beetle Pu mite from field acquisition crinosity bark beetle larva or pupal cell, be placed in culture dish, in 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, to raise 6 days to 13 days, the bark beetle Pu mite that raising obtains is as original seed;
The expansion of the 3rd step bark beetle Pu mite is numerous: the mung bean that the interior transparent kind of skin that has adzuki bean weevil Larva and a pupa needled is inserted in gauze bag, with bark beetle Pu mite original seed and Hosts adzuki bean weevil larva or the access of the pupa ratio of 1: 4, the gauze bag of inoculation is inserted in the crisper with sand net, put into again 20 ℃ to 30 ℃ of temperature, relative moisture 40% to 70% time, raise 6 days to 13 days, so just breed out a large amount of bark beetle Pu mites.
2. the method with adzuki bean weevil mass rearing bark beetle Pu mite according to claim 1, the diameter that it is characterized in that culture dish is 9 centimetres.
3. the method with adzuki bean weevil mass rearing bark beetle Pu mite according to claim 2, is characterized in that culture dish scribbles vaseline around.
4. according to the method with adzuki bean weevil mass rearing bark beetle Pu mite described in claim 1 or 2 or 3, the length that it is characterized in that crisper is that 30 centimetres, width are 20 centimetres, highly are 8 centimetres, lid and both sides band sand net.
5. according to claim 1 or 2 or the described method with adzuki bean weevil mass rearing bark beetle Pu mite, it is characterized in that in the 3rd step, the length of gauze bag is that 5 centimetres, width are 7 centimetres, and the addition of mung bean is 1/4th capacity of gauze bag, and gauze bag is 80 order to 100 objects.
6. the method with adzuki bean weevil mass rearing bark beetle Pu mite according to claim 4, it is characterized in that in the 3rd step, the length of gauze bag is that 5 centimetres, width are 7 centimetres, and the addition of mung bean is 1/4th capacity of gauze bag, and gauze bag is 80 order to 100 objects.
7. the application in bark beetle biological control of insect pests according to bark beetle Pu mite that in claim 1 to 6, arbitrarily the method with adzuki bean weevil mass rearing bark beetle Pu mite described in claim obtains, it is characterized in that when breed a large amount of output of bark beetle Pu mite time, gauze bag one side is hammered into apricot and has by the brill channel of bark beetle.
8. the application of the bark beetle Pu mite described in 7 in bark beetle biological control of insect pests as requested, is characterized in that the time that bark beetle Pu mite discharges in field, South Sinkiang, Xinjiang is: every year at the beginning of by the end of April to 6 month or by the end of August to by the end of September.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210043792.1A CN102524194B (en) | 2012-02-26 | 2012-02-26 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210043792.1A CN102524194B (en) | 2012-02-26 | 2012-02-26 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102524194A CN102524194A (en) | 2012-07-04 |
| CN102524194B true CN102524194B (en) | 2014-03-19 |
Family
ID=46333247
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210043792.1A Expired - Fee Related CN102524194B (en) | 2012-02-26 | 2012-02-26 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102524194B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396886B (en) * | 2014-10-22 | 2016-08-31 | 云南大学 | A kind of Callosobruchus maculatus larva large-scale breeding method |
| CN105230576B (en) * | 2015-11-06 | 2017-12-26 | 天津拓华生物科技有限责任公司 | A kind of method using mung bean large-scale production Callosobruchus chinensis larva of peeling |
| CN105284741A (en) * | 2015-11-06 | 2016-02-03 | 天津拓华生物科技有限责任公司 | Mass propagation method for larvae of callosobruchus chinensis linnaeus |
| CN106472428A (en) * | 2016-09-28 | 2017-03-08 | 天津拓华生物科技有限责任公司 | A kind of utilization fruit bat and the method for bean weevil mixing raising ladybug |
| CN107279073B (en) * | 2017-08-17 | 2021-04-23 | 广州市林业和园林科学研究院 | Indoor breeding method for coconut leaf moths |
| CN117281086A (en) * | 2022-05-10 | 2023-12-26 | 广西壮族自治区林业科学研究院 | Method for breeding pyemotes by using mature larva of Chilo suppressalis and application |
-
2012
- 2012-02-26 CN CN201210043792.1A patent/CN102524194B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 于丽辰.肤小蠹蒲螨的生物学及温度对其生长发育、繁殖的影响.《走向21世纪的中国昆虫学——中国昆虫学会2000年学术年会论文集》.2000, |
| 于丽辰等.我国新天敌资源——小蠹蒲螨形态与生物学研究.《蛛形学报》.1997,(第1期), |
| 张鲁豫等.轮台县杏树小蠹虫天敌种类及小蠹蒲螨生物学特性研究初探.《新疆农业大学学报》.2011,(第5期),508页－511页. |
| 我国新天敌资源——小蠹蒲螨形态与生物学研究;于丽辰等;《蛛形学报》;19970130(第1期);46－52页 * |
| 肤小蠹蒲螨的生物学及温度对其生长发育、繁殖的影响;于丽辰;《走向21世纪的中国昆虫学——中国昆虫学会2000年学术年会论文集》;20001230;1031－1032页 * |
| 轮台县杏树小蠹虫天敌种类及小蠹蒲螨生物学特性研究初探;张鲁豫等;《新疆农业大学学报》;20110530(第5期);508页－511页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102524194A (en) | 2012-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102524194B (en) | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests | |
| CN105309142B (en) | Sexual propagation method for gastrodia elata | |
| CN104041377A (en) | Intercropping method suitable for desert area | |
| CN105248222A (en) | Longan planting method | |
| CN105123257A (en) | Container seedling method for oil peony and seedling medium | |
| CN103518518A (en) | Method for planting bitter gourds | |
| CN103392458A (en) | Planting technology for killing and restraining root-knot nematodes | |
| CN102823419A (en) | Method for preventing natural grassland from exotic plant invasion | |
| CN104756804A (en) | Method for preventing and controlling peach fruit moths in apples by means of desinsectization plants | |
| CN102812928B (en) | Breeding method of pyemotes tritici | |
| CN101999416B (en) | Nematode controlling reagent and production flow thereof | |
| CN102550492B (en) | Artificial mass raising method for agasicles hygrophila | |
| CN103262767B (en) | Cotton aphid control method | |
| CN104145668A (en) | Integrated control method for black spot disease in close planting jujube orchard | |
| CN102405804A (en) | Biological control technique of corn borer | |
| Usman et al. | Media steaming and coco-coir enhance growth of rough lemon (Citrus jambhiri L.) stock. | |
| CN104272943A (en) | Cultivation method for celery | |
| CN103404356B (en) | A kind of afforestation method of beech tree cuttage seedling | |
| CN104094812A (en) | Three-dimensional circular desert control method | |
| CN110199761A (en) | A kind of improvement implantation methods of Kingsoft green bud | |
| CN110692600B (en) | Artificial feed for anoplophora chinensis larvae and subculturing method of anoplophora chinensis | |
| CN103621493B (en) | The biological control method of chestnut unguiculus mite | |
| CN102907285A (en) | Comprehensive treatment method for preventing pine wood nematode disease from spreading | |
| CN103535252A (en) | Compound organic-soil planting farm | |
| CN103340185B (en) | Method for evaluating parasitic effect of procecidochares utilis on ageratina adenophora |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140319 Termination date: 20150226 |
|
| EXPY | Termination of patent right or utility model |