CN117281086A - Method for breeding pyemotes by using mature larva of Chilo suppressalis and application - Google Patents
Method for breeding pyemotes by using mature larva of Chilo suppressalis and application Download PDFInfo
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- 241000238706 Pyemotes Species 0.000 title claims abstract description 86
- 230000001488 breeding effect Effects 0.000 title claims abstract description 35
- 238000009395 breeding Methods 0.000 title claims abstract description 34
- 241000426497 Chilo suppressalis Species 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 239000004745 nonwoven fabric Substances 0.000 claims abstract description 20
- 241001122767 Theaceae Species 0.000 claims abstract description 13
- 241000238631 Hexapoda Species 0.000 claims abstract description 11
- 206010002091 Anaesthesia Diseases 0.000 claims abstract description 9
- 230000037005 anaesthesia Effects 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000002023 wood Substances 0.000 claims abstract description 7
- 210000001015 abdomen Anatomy 0.000 claims abstract description 6
- 238000007605 air drying Methods 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 claims abstract description 3
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 241000209140 Triticum Species 0.000 claims description 22
- 235000021307 Triticum Nutrition 0.000 claims description 22
- 230000002265 prevention Effects 0.000 claims description 14
- 241000607479 Yersinia pestis Species 0.000 claims description 12
- 239000003921 oil Substances 0.000 claims description 12
- 241000526900 Camellia oleifera Species 0.000 claims description 11
- 239000010495 camellia oil Substances 0.000 claims description 10
- 239000005907 Indoxacarb Substances 0.000 claims description 6
- 239000005931 Spirotetramat Substances 0.000 claims description 6
- CXEGAUYXQAKHKJ-NSBHKLITSA-N emamectin B1a Chemical compound C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](NC)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 CXEGAUYXQAKHKJ-NSBHKLITSA-N 0.000 claims description 6
- VBCVPMMZEGZULK-NRFANRHFSA-N indoxacarb Chemical compound C([C@@]1(OC2)C(=O)OC)C3=CC(Cl)=CC=C3C1=NN2C(=O)N(C(=O)OC)C1=CC=C(OC(F)(F)F)C=C1 VBCVPMMZEGZULK-NRFANRHFSA-N 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- CLSVJBIHYWPGQY-GGYDESQDSA-N spirotetramat Chemical compound CCOC(=O)OC1=C(C=2C(=CC=C(C)C=2)C)C(=O)N[C@@]11CC[C@H](OC)CC1 CLSVJBIHYWPGQY-GGYDESQDSA-N 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
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- 238000010008 shearing Methods 0.000 claims description 2
- 230000003405 preventing effect Effects 0.000 abstract description 18
- 230000001276 controlling effect Effects 0.000 description 19
- 230000000694 effects Effects 0.000 description 8
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- 230000035764 nutrition Effects 0.000 description 2
- 241000238876 Acari Species 0.000 description 1
- 241001481710 Cerambycidae Species 0.000 description 1
- 241000098289 Cnaphalocrocis medinalis Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses a method for breeding pyemotes by using mature larvae of large wax moth and application of the method in preventing and controlling oil tea insect Li Tiao moths, wherein the method for breeding the pyemotes by using the mature larvae of large wax moth comprises the following steps: (1) treatment of the mature larva of the Chilo suppressalis: placing the mature larva of the Chilo suppressalis in 75% alcohol for anesthesia for 4-5min, taking out, soaking in clear water for 1min, and air-drying for later use; (2) propagation of Typhaga oleifera: placing 10 heads of treated large wax moth mature larvae and 1 head of a commercially available pyemote strain source with a belly expansion body in a heat-sealing non-woven fabric filter bag breeding device with a length of 8cm multiplied by 6cm, sealing by a sealing machine, vertically placing the sealed heads in a feeding box with a bottom filled with fermented wood chips, smearing fresh powder on the inner wall of the feeding box before placing, and covering a breathable box cover on a breeding frame; culturing at room temperature for 5d to breed Typhaga oleifera. The invention only carries out simple anesthesia treatment on the mature larva of the Chilo suppressalis, thereby improving the propagation efficiency of the pyemotes and reducing the propagation cost.
Description
Technical Field
The invention relates to the technical field of biological pest control, in particular to a method for breeding pyemotes by using mature larvae of cnaphalocrocis medinalis and application of the method in controlling camellia oleifera pest Li Tiao moths.
Background
Pyemotes are natural enemies of various boring pests. At present, the parasitic effects of the pyemotes on the longicorn beetles, bai Fuxiao beetles, japanese backbones beetles, six-star leopard beetles, mediterranean powder borers and the like are found to reach more than 80 percent. Experiments of parasitic coco moths of the pyemotes of wheat show that the parasitic rate of the pyemotes to the coco moths can reach 100 percent. Thus, pyemotes are a good natural enemy.
To date, the propagation of pyemotes requires alternative hosts, the choice of which is still the main factor determining the propagation effect of the pyemotes. The most successful alternative hosts used at this stage are mainly: the larvae or primary pupae of wheat moth, corn weevil, bean weevil, yellow meal worm pupae, fish eggs, bee larvae and pupae, larva of Chilo suppressalis of 3-4 ages, etc. The technology of breeding the pyemotes on a large scale by utilizing some hosts has been broken through, and the commodity of the pyemotes is also marketed. Some of the alternative hosts such as the corn weevils and the bean weevils have the conditions of unrealized artificial breeding, low breeding efficiency, high breeding cost and complex operation, and limit large-scale breeding. The replacement hosts such as yellow meal beetles, fish eggs, bee larvae, pupae and the like belong to different purposes from the Li Tiao moths prevented and treated at this time, and the pyemotes propagated by the replacement hosts are used for preventing and treating Li Tiao moths with possibility of degeneration. Among 2 alternative hosts of the wheat moth and the wax moth, the wheat moth should be the optimal host for preventing and controlling Li Tiao wheat moth and pyemotes, and can be bred in large scale, but compared with the wax moth, the breeding process is relatively complex, individuals are smaller, offspring are fewer, the relative cost is relatively high, and the production procedure is relatively complex. The breeding technology difficulty of the Chilo suppressalis is broken through, a set of simple and efficient breeding technology process is formed, and the annual large-scale breeding can be realized.
Currently, researchers have bred Typhagus Cochinchila by using larvae of Chilo suppressalis 3-4 years old. Compared with the larva of the wax moth of 3-4 ages, the mature larva of the wax moth of more than 5 ages has larger body type and more abundant nutrition components, and the number of the pyemotes propagated by the single mature larva of the wax moth is more than 4 times of that of the larva of the wax moth of 3-4 ages. However, the mature larva of the Chilo suppressalis is too active, and if the larva is not treated, the larva is directly used for breeding the pyemotes, and the breeding effect is poor. Therefore, the technology of overactivity and non-mortality of the aged larvae of the Chilo suppressalis is broken through, so that the propagation process of the pyemotes is really simplified, the propagation cost is reduced, and the nondegradable pyemotes are produced for biological control.
Disclosure of Invention
In view of the above, the invention provides a method for breeding pyemotes by using the mature larva of Chilo suppressalis and application thereof in preventing and controlling oil tea insect Li Tiao wheat moth.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for breeding pyemotes by using mature larvae of Chilo suppressalis, which is characterized by comprising the following steps:
(1) Treatment of the mature larvae of Chilo suppressalis: placing the mature larva of the Chilo suppressalis in 75% alcohol for anesthesia for 4-5min, taking out, soaking in clear water for 1min, and air-drying for later use; (2) propagation of Typhaga oleifera: placing 10 heads of treated large wax moth mature larvae and 1 head of a commercially available pyemote strain source with a belly expansion body in a heat-sealing non-woven fabric filter bag breeding device with a length of 8cm multiplied by 6cm, sealing by a sealing machine, vertically placing the sealed heads in a feeding box with a bottom filled with fermented wood chips, smearing fresh powder on the inner wall of the feeding box before placing, and covering a breathable box cover on a breeding frame; culturing at room temperature for 5d to breed Typhaga oleifera.
Preferably, the pyemotes obtained by the method are applied to prevention and control or auxiliary prevention and control of the camellia oleifera insect Li Tiao wheat moth.
Preferably, the pyemotes obtained by the method are applied to the preparation of products for preventing and controlling or assisting in preventing and controlling oil tea insect Li Tiao moths.
A method for preventing or assisting in preventing and controlling tea-oil tree insect Li Tiao wheat moth is characterized in that the above-mentioned pyemotes are released onto plants or into spaces containing insect Li Tiao wheat moth, so as to realize the prevention and control of tea-oil tree insect Li Tiao wheat moth.
Preferably, the release method of the pyemotes comprises the following steps: fixing the non-woven fabric bag of the bred pyemotes on the trunk of the tea-oil tree by using a nailing gun, and shearing an opening of 0.5cm on the side surface of the non-woven fabric bag for the pyemotes to climb out; each camellia oleifera releases a bag of pyemotes.
Preferably, before the pyemotes released in the camellia oleifera forest are used for preventing and controlling Li Tiao moths, spraying a mixed solution of 3.5% emamectin benzoate, 6.5% indoxacarb and 22.4% spirotetramat in a 1:1:1 ratio for 1000-2000 times to a prevention and control area for preventing and controlling Li Tiao moths once; 10-15d after the prevention and control of the pesticide is carried out, and then the pyemotes are released to prevent and control the remained Li Tiao moths.
The invention has the technical advantages and beneficial effects that:
1. the wax moth belongs to the Lepidoptera moth family, has similar habit with Li Tiao moth belonging to the Lepidoptera moth family, and the Typhaga oleifera bred by mature larvae has smaller degeneration probability than the Typhaga oleifera bred by other insects, and has better control effect on Li Tiao moth.
2. The mature larva of the Chilo suppressalis has larger body size and more abundant nutrition components, and the number of the pyemotes propagated by the single mature larva of the Chilo suppressalis is more than 4 times that of the larva of the Chilo suppressalis with the age of 3-4. The invention only needs a simple anesthesia treatment process for the mature larva of the Chilo suppressalis, greatly improves the propagation efficiency of the pyemotes, and has lower propagation cost.
3. The heat-sealing non-woven fabric bag used by the invention is used as a propagation device and a release device of the pyemotes, so that the propagation technology process of the pyemotes is further simplified, and the pyemotes are prevented from escaping by using the pyemotes, thereby protecting the pyemotes propagation production personnel, and simultaneously, the cost is lower, and the method is more environment-friendly and efficient.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the embodiments, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
(1) Treatment of the mature larvae of Chilo suppressalis: placing the mature larva of the Chilo suppressalis in 75% alcohol for anesthesia for 4min, taking out, immersing in clear water for 1min, and airing for later use;
(2) Propagation of pyemotes: placing 10 heads of treated large wax moth mature larvae and 1 head of a commercially available pyemote strain source with a belly expansion body in a heat-sealing non-woven fabric filter bag breeding device with a length of 8cm multiplied by 6cm, sealing by a sealing machine, vertically placing the sealed heads in a feeding box with a bottom filled with fermented wood chips, smearing fresh powder on the inner wall of the feeding box before placing, and covering a breathable box cover on a breeding frame; after breeding for 6d under the room temperature condition, the wheat moth is used for preventing and controlling Li Tiao wheat moth;
(3) Release control of pyemotes Li Tiao moths: before releasing pyemotes in oil tea forests to prevent Li Tiao moths, spraying a mixed solution of 3.5% emamectin benzoate, 6.5% indoxacarb and 22.4% spirotetramat in a 1:1:1 ratio for 1000 times to prevent Li Tiao moths once in a prevention area; 11d after the prevention and control of the pesticide is sprayed, releasing the pyemotes to prevent and control the rest Li Tiao moths; when Pu Man is released, the non-woven fabric bag of the bred pyemotes is fixed on the trunk of the tea-oil tree by a nail gun, and an opening of 0.5cm is cut on the side surface of the non-woven fabric bag for the pyemotes to climb out; each camellia oleifera releases a bag of pyemotes.
The test results show (table 1) that the chemical agent can release the pyemotes after preventing and controlling, and the prevention and control rate can reach 99.54 percent.
TABLE 1 statistical Table of Typhaga-mite+chemical control Li Tiao Male moth effects
Example 2
(1) Treatment of the mature larvae of Chilo suppressalis: placing the mature larva of the Chilo suppressalis in 75% alcohol for anesthesia for 5min, taking out, immersing in clear water for 1min, and airing for later use;
(2) Propagation of pyemotes: placing 10 heads of treated large wax moth mature larvae and 1 head of a commercially available pyemote strain source with a belly expansion body in a heat-sealing non-woven fabric filter bag breeding device with a length of 8cm multiplied by 6cm, sealing by a sealing machine, vertically placing the sealed heads in a feeding box with a bottom filled with fermented wood chips, smearing fresh powder on the inner wall of the feeding box before placing, and covering a breathable box cover on a breeding frame; after breeding for 5 days at room temperature, the plant is used for preventing and controlling Li Tiao wheat moth;
(3) Release control of pyemotes Li Tiao moths: before releasing pyemotes in oil tea forests to prevent Li Tiao moths, spraying a mixed solution of 3.5% emamectin benzoate, 6.5% indoxacarb and 22.4% spirotetramat in a 1:1:1 ratio for 2000 times to prevent Li Tiao moths once in a prevention area; 12d after the prevention and control of the pesticide is carried out, the pyemotes are released to prevent and control the remained Li Tiao moths; when Pu Man is released, the non-woven fabric bag of the bred pyemotes is fixed on the trunk of the tea-oil tree by a nail gun, and an opening of 0.5cm is cut on the side surface of the non-woven fabric bag for the pyemotes to climb out; each camellia oleifera releases a bag of pyemotes.
Test results show that the cattail mites are released after the chemical agent is used for controlling, so that a good effect can be achieved, and the control rate reaches 99.56%.
Example 3
(1) Treatment of the mature larvae of Chilo suppressalis: placing the mature larva of the Chilo suppressalis in 75% alcohol for anesthesia for 4.5min, taking out, immersing in clear water for 1min, and airing for standby;
(2) Propagation of pyemotes: placing 10 heads of treated large wax moth mature larvae and 1 head of a commercially available pyemote strain source with a belly expansion body in a heat-sealing non-woven fabric filter bag breeding device with a length of 8cm multiplied by 6cm, sealing by a sealing machine, vertically placing the sealed heads in a feeding box with a bottom filled with fermented wood chips, smearing fresh powder on the inner wall of the feeding box before placing, and covering a breathable box cover on a breeding frame;
(3) The pyemotes are bred for 6 days at room temperature and then used for preventing and controlling Li Tiao wheat moth; before releasing pyemotes in oil tea forests to prevent Li Tiao moths, spraying a mixed solution of 3.5% emamectin benzoate, 6.5% indoxacarb and 22.4% spirotetramat in a 1:1:1 ratio for 1500 times to prevent Li Tiao moths once in a prevention area; when Pu Man is released, the non-woven fabric bag of the bred pyemotes is fixed on the trunk of the tea-oil tree by a nail gun, and an opening of 0.5cm is cut on the side surface of the non-woven fabric bag for the pyemotes to climb out; each camellia oleifera releases a bag of pyemotes.
Test results show that the chemical control and prevention can be carried out before releasing the pyemotes, so that a better effect can be achieved, and the control rate reaches 99.55%.
Comparative example 1
(1) Treatment of the mature larvae of Chilo suppressalis: placing the mature larva of the Chilo suppressalis in 75% alcohol for anesthesia for 4min, taking out, immersing in clear water for 1min, and airing for later use;
(2) Propagation of pyemotes: placing the treated mature larva of Chilo suppressalis 10 head and the commercially available Typhagus cochinchinensis seed source 1 head with bulked body in a heat-sealing non-woven fabric filter bag breeding device with a length of 8cm multiplied by 6cm, sealing with a sealing machine, vertically placing in a feeding box with fermented wood dust filled at the bottom, smearing fresh powder on the inner wall of the feeding box before placing, and covering a breathable box cover on a breeding frame.
(3) The pyemotes are bred for 6 days at room temperature and then used for preventing and controlling Li Tiao wheat moth; when Pu Man is released, the non-woven fabric bag of the bred pyemotes is fixed on the trunk of the tea-oil tree by a nail gun, and an opening of 0.5cm is cut on the side surface of the non-woven fabric bag for the pyemotes to climb out; each camellia oleifera releases a bag of pyemotes.
The test results show (Table 2) that, because the woodland is an open space, the actual control capacity of the oil tea insect pest Li Tiao moths without chemical control and combination of the pyemotes can only reach 80.39 percent due to the environmental factors such as phoenix, water, light, natural enemies and the like, the natural enemies and the self host searching capacity and the like in the process of searching the hosts by the pyemotes.
TABLE 2 statistical Table of the effect of Typhaga oleifera control Li Tiao on wheat moth
Comparative example 2
A method for chemically controlling and controlling oil tea pests Li Tiao wheat moth comprises spraying a mixed solution of 3.5% emamectin benzoate, 6.5% indoxacarb and 22.4% spirotetramat at a ratio of 1:1:1 for one time to control Li Tiao wheat moth.
The test results show (Table 3) that the chemical agent can prevent and control oil tea insect Li Tiao wheat moth up to 84.65%.
Table 3 statistics of chemical control effects of Li Tiao on wheat moth
As can be seen from comparison of tables 1-3, the actual control capacity can only reach 80.39% due to environmental factors such as phoenix, water, light and natural enemies and the like and factors such as natural enemies and the capacity of searching the host by the pyemotes in the process of searching the host due to the fact that the woodland is an open space; in addition, as Li Tiao wheat moth is hidden in the oil tea tip, the prevention and control effect of the chemical agent can only reach 84.65%. The test result of the invention shows that the chemical agent releases the pyemotes after preventing and controlling, thereby obtaining better preventing and controlling effect, and the preventing and controlling effect reaches more than 99.54 percent.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A method for breeding pyemotes by using mature larvae of Chilo suppressalis, which is characterized by comprising the following steps: (1) treatment of the mature larva of the Chilo suppressalis: placing the mature larva of the Chilo suppressalis in 75% alcohol for anesthesia for 4-5min, taking out, soaking in clear water for 1min, and air-drying for later use; (2) propagation of Typhaga oleifera: placing 10 heads of treated large wax moth mature larvae and 1 head of a commercially available pyemote strain source with a belly expansion body in a heat-sealing non-woven fabric filter bag breeding device with a length of 8cm multiplied by 6cm, sealing by a sealing machine, vertically placing the sealed heads in a feeding box with a bottom filled with fermented wood chips, smearing fresh powder on the inner wall of the feeding box before placing, and covering a breathable box cover on a breeding frame; culturing at room temperature for 5d to breed Typhaga oleifera.
2. Use of a pyemote obtained according to the method of claim 1 for controlling or assisting in controlling the camellia oleifera pest Li Tiao moth.
3. Use of a pyemote obtained according to the method of claim 1 for the preparation of a product for controlling or co-controlling camellia oleifera pest Li Tiao moth.
4. A method for controlling or assisting in controlling tea-oil tree pest Li Tiao moth, which is characterized in that the pyemotes of claim 1 are released onto plants or into spaces containing pest Li Tiao moth, so as to control tea-oil tree pest Li Tiao moth.
5. The method for controlling or assisting in controlling oil tea insect pests Li Tiao wheat moth according to claim 4, wherein the release method of the pyemotes is as follows: fixing the non-woven fabric bag of the bred pyemotes on the trunk of the tea-oil tree by using a nailing gun, and shearing an opening of 0.5cm on the side surface of the non-woven fabric bag for the pyemotes to climb out; each camellia oleifera releases a bag of pyemotes.
6. The method for controlling or assisting in controlling oil tea pests Li Tiao moths according to any one of claims 4 to 5, wherein before releasing pyemotes in oil tea forests to control Li Tiao moths, spraying a mixed solution of 3.5% emamectin benzoate, 6.5% indoxacarb and 22.4% spirotetramat in a ratio of 1:1:1 for 1000-2000 times to control Li Tiao moths once; 10-15d after the prevention and control of the pesticide is carried out, and then the pyemotes are released to prevent and control the remained Li Tiao moths.
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CN101569297B (en) * | 2009-06-08 | 2011-11-16 | 河北省农林科学院昌黎果树研究所 | Breeding method of pyemotes zhonghuajia and application thereof in pest biological control |
CN101911926B (en) * | 2010-08-17 | 2012-06-27 | 段东红 | Pyemotes spraying structure as well as manufacturing method and release device thereof |
CN102524194B (en) * | 2012-02-26 | 2014-03-19 | 新疆农业大学 | Method for artificially propagating pyemotes bark beetle through Callosobruchus chinensis and application in biological control of bark beetle pests |
CN102812928B (en) * | 2012-08-02 | 2013-11-27 | 北京林业大学 | Breeding method of pyemotes tritici |
CN111264473B (en) * | 2020-02-18 | 2021-09-28 | 广西壮族自治区林业科学研究院 | Method for breeding Exorista bigelovii by utilizing galleria mellonella |
CN111771819B (en) * | 2020-07-09 | 2022-08-05 | 武汉市园林科学研究院 | Application of galleria mellonella larva as intermediate host in artificial breeding of dastarcus helophoroides |
CN111887207B (en) * | 2020-07-09 | 2022-08-02 | 武汉市园林科学研究院 | Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects |
CN112931416A (en) * | 2021-02-24 | 2021-06-11 | 中国林业科学研究院森林生态环境与保护研究所 | Method for breeding pyemotes and method for using pyemotes to control boring insects |
CN215684304U (en) * | 2021-08-13 | 2022-02-01 | 贵州大学 | Breeding and expanding propagation device for Chinese beetles and pyemotes |
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