CN104017898B - 快速检测hla-b*5801等位基因的引物、试剂盒及方法 - Google Patents
快速检测hla-b*5801等位基因的引物、试剂盒及方法 Download PDFInfo
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- CN104017898B CN104017898B CN201410292967.1A CN201410292967A CN104017898B CN 104017898 B CN104017898 B CN 104017898B CN 201410292967 A CN201410292967 A CN 201410292967A CN 104017898 B CN104017898 B CN 104017898B
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- 102000006390 HLA-B Antigens Human genes 0.000 title claims abstract description 51
- 108010058607 HLA-B Antigens Proteins 0.000 title claims abstract description 51
- 238000001514 detection method Methods 0.000 title claims abstract description 29
- 238000012360 testing method Methods 0.000 title claims abstract description 26
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- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- OBRMNDMBJQTZHV-UHFFFAOYSA-N cresol red Chemical compound C1=C(O)C(C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(C)C(O)=CC=2)=C1 OBRMNDMBJQTZHV-UHFFFAOYSA-N 0.000 claims description 4
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- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Immunology (AREA)
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- Molecular Biology (AREA)
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- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
引物编号 | 序列号 | 引物序列 |
58PF01 | SEQ ID NO.1 | AAC ATG AAG GCC TCC GCC |
58PR01 | SEQ ID NO.2 | CCG CGC GCT GCA GCG TCG |
58PF02 | SEQ ID NO.3 | CGC GAGTCC GAG GAC GGA |
58PR02 | SEQ ID NO.4 | TTC ATG TTC CGT GTC TCC CC |
58PF03 | SEQ ID NO.5 | ACG TGA CCC ACC ACC CCG |
58PR03 | SEQ ID NO.6 | GAA GGT TCT ATC TCC TGC TGG |
NCXF | SEQ ID NO.7 | AGC GAG CAT CCC CCA AAG TT |
NCXR | SEQ ID NO.8 | GGG CAC GAA GGC TCA TCA TT |
Claims (7)
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CN201410292967.1A CN104017898B (zh) | 2014-06-25 | 2014-06-25 | 快速检测hla-b*5801等位基因的引物、试剂盒及方法 |
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CN201410292967.1A CN104017898B (zh) | 2014-06-25 | 2014-06-25 | 快速检测hla-b*5801等位基因的引物、试剂盒及方法 |
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CN104017898A CN104017898A (zh) | 2014-09-03 |
CN104017898B true CN104017898B (zh) | 2016-04-27 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104293932B (zh) * | 2014-09-26 | 2017-02-15 | 陕西佰美基因股份有限公司 | 一种基于实时荧光pcr检测hla‑b*5801等位基因的方法 |
CN104232781B (zh) * | 2014-09-26 | 2017-04-19 | 陕西佰美基因股份有限公司 | 一种检测HLA‑B*5801等位基因的TaqMan探针实时荧光PCR方法 |
CN105950766B (zh) * | 2016-06-30 | 2019-12-06 | 江苏伟禾生物科技有限公司 | 一种用于检测hla-b*5801等位基因的引物组及试剂盒 |
CN108624676B (zh) * | 2018-05-23 | 2022-04-01 | 健路生物科技(苏州)有限公司 | 用于检测hla-b*5801等位基因的试剂盒及其检测方法和应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
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US7470513B2 (en) * | 2003-11-10 | 2008-12-30 | Academia Sinica | Risk assessment for adverse drug reactions |
AU2007249225B2 (en) * | 2006-05-11 | 2013-06-27 | Academia Sinica | HLA alleles associated with adverse drug reactions and methods for detecting such |
CN101760515A (zh) * | 2008-12-24 | 2010-06-30 | 世基生物医学股份有限公司 | 基因检测试剂组 |
CN103484533B (zh) * | 2012-06-08 | 2015-09-09 | 复旦大学附属华山医院 | 检测hla-b*5801等位基因的方法 |
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Denomination of invention: Primers, Kits, and Methods for Rapid Detection of HLA-B * 5801 Allele Effective date of registration: 20230719 Granted publication date: 20160427 Pledgee: Industrial Bank Co.,Ltd. Shanghai Nanhui Branch Pledgor: SHANGHAI TISSUEBANK MEDICAL LABORATORY Co.,Ltd.|SHENZHEN TISSUEBANK PRECISION MEDICINE CO.,LTD.|SHANGHAI TISSUEBANK BIOTECHNOLOGY Co.,Ltd.|Shanghai dishuobeiken Gene Technology Co.,Ltd.|SHANGHAI TISSUEBANK BIOTECHNOLOGY CO.,LTD. Registration number: Y2023310000384 |
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Granted publication date: 20160427 Pledgee: Industrial Bank Co.,Ltd. Shanghai Nanhui Branch Pledgor: SHANGHAI TISSUEBANK MEDICAL LABORATORY Co.,Ltd.|SHENZHEN TISSUEBANK PRECISION MEDICINE CO.,LTD.|SHANGHAI TISSUEBANK BIOTECHNOLOGY Co.,Ltd.|Shanghai dishuobeiken Gene Technology Co.,Ltd.|SHANGHAI TISSUEBANK BIOTECHNOLOGY CO.,LTD. Registration number: Y2023310000384 |