CN1039231C - 因子vii的纯化 - Google Patents

因子vii的纯化 Download PDF

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Publication number
CN1039231C
CN1039231C CN94191833A CN94191833A CN1039231C CN 1039231 C CN1039231 C CN 1039231C CN 94191833 A CN94191833 A CN 94191833A CN 94191833 A CN94191833 A CN 94191833A CN 1039231 C CN1039231 C CN 1039231C
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CN
China
Prior art keywords
fvii
buffer solution
purification
activation
nacl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN94191833A
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English (en)
Chinese (zh)
Other versions
CN1121723A (zh
Inventor
T·朱尔根森
A·H·佩德森
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Novo Nordisk AS
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Novo Nordisk AS
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Publication of CN1121723A publication Critical patent/CN1121723A/zh
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Publication of CN1039231C publication Critical patent/CN1039231C/zh
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Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Catalysts (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN94191833A 1993-03-31 1994-03-24 因子vii的纯化 Expired - Fee Related CN1039231C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK93382A DK38293D0 (da) 1993-03-31 1993-03-31 Fremstilling af proteiner
DK382/93 1993-03-31

Publications (2)

Publication Number Publication Date
CN1121723A CN1121723A (zh) 1996-05-01
CN1039231C true CN1039231C (zh) 1998-07-22

Family

ID=8092871

Family Applications (1)

Application Number Title Priority Date Filing Date
CN94191833A Expired - Fee Related CN1039231C (zh) 1993-03-31 1994-03-24 因子vii的纯化

Country Status (16)

Country Link
US (1) US5700914A (OSRAM)
EP (1) EP0691984A1 (OSRAM)
JP (1) JPH08508264A (OSRAM)
CN (1) CN1039231C (OSRAM)
AU (1) AU677309B2 (OSRAM)
CA (1) CA2159313A1 (OSRAM)
CZ (1) CZ253395A3 (OSRAM)
DK (1) DK38293D0 (OSRAM)
FI (1) FI954649A7 (OSRAM)
HU (1) HUT72712A (OSRAM)
IL (1) IL109164A0 (OSRAM)
NO (1) NO953883L (OSRAM)
PL (1) PL310887A1 (OSRAM)
TW (1) TW278079B (OSRAM)
WO (1) WO1994022905A1 (OSRAM)
ZA (1) ZA941956B (OSRAM)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19538716A1 (de) * 1995-10-18 1997-04-24 Behringwerke Ag Verfahren zur Quantifizierung von aktiviertem Gerinnungsfaktor VII (FVIIa)
DE19538715A1 (de) 1995-10-18 1997-04-30 Behringwerke Ag Verfahren zur Reinigung von Faktor VII und aktiviertem Faktor VII
AU760048B2 (en) 1998-05-06 2003-05-08 Genentech Inc. Protein purification by ion exchange chromatography
AT408613B (de) 1998-06-17 2002-01-25 Immuno Ag Pharmazeutisches faktor vii-präparat
NZ521257A (en) 2000-02-11 2004-10-29 Maxygen Aps Factor VII or VIIa-like molecules
BR0215216A (pt) 2001-12-21 2004-11-16 Novo Nordisk Healthcare Ag Composição aquosa lìquida, método para preparar uma composição aquosa lìquida de um polipeptìdeo de fator vii, uso de uma composição aquosa lìquida, e, método para o tratamento de uma sìndrome de resposta ao fator vii
US20040009918A1 (en) * 2002-05-03 2004-01-15 Hanne Nedergaard Stabilised solid compositions of modified factor VII
KR20050013148A (ko) * 2002-06-21 2005-02-02 노보 노르디스크 에이/에스 인자 ⅶ 폴리펩티드의 안정화된 고체 조성물
MXPA05009770A (es) * 2003-03-18 2005-11-17 Novo Nordisk Healthcare Ag Metodo para la produccion de serina proteasas que contienen residuos de gla.
US7897734B2 (en) 2003-03-26 2011-03-01 Novo Nordisk Healthcare Ag Method for the production of proteins
CN1780638B (zh) 2003-05-23 2010-04-14 诺和诺德医疗保健公司 氧化硅涂层玻璃作为容器内壁材料的用途及以该材料作内壁的容器
WO2004112828A1 (en) * 2003-06-25 2004-12-29 Novo Nordisk Health Care Ag Liquid composition of factor vii polypeptides
JP5306597B2 (ja) * 2003-07-01 2013-10-02 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 第ｖｉｉ因子ポリペプチドの液体水性薬学的組成物
JP5653572B2 (ja) 2003-08-14 2015-01-14 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 第ｖｉｉ因子ポリペプチドの液状水性医薬組成物
ES2926359T3 (es) 2003-12-01 2022-10-25 Novo Nordisk Healthcare Ag Filtración de virus de composiciones líquidas del Factor VII
ATE507822T1 (de) * 2003-12-19 2011-05-15 Novo Nordisk Healthcare Ag Stabilisierte zusammensetzungen von faktor-vii- polypeptiden
WO2006067230A1 (en) * 2004-12-23 2006-06-29 Novo Nordisk Health Care Ag Reduction of the content of protein contaminants in compositions comprising a vitamin k-dependent protein of interest
US20090047723A1 (en) * 2005-01-14 2009-02-19 Bayer Healthcare Llc Method for purification of factor vii
EP1907540B1 (en) * 2005-07-22 2012-12-19 Bayer HealthCare LLC In-solution activation of factor vii
ES2779992T3 (es) * 2005-12-20 2020-08-21 Univ Duke Métodos y composiciones para suministrar agentes activos con propiedades farmacológicas potenciadas
US20130172274A1 (en) 2005-12-20 2013-07-04 Duke University Methods and compositions for delivering active agents with enhanced pharmacological properties
US8841255B2 (en) 2005-12-20 2014-09-23 Duke University Therapeutic agents comprising fusions of vasoactive intestinal peptide and elastic peptides
CA2953975C (en) 2008-06-27 2019-11-26 Duke University Therapeutic agents comprising elastin-like peptides
CN103539852B (zh) * 2012-07-12 2015-08-12 上海泰龙生物医药科技有限公司 一种从细胞培养液中分离纯化重组人凝血八因子的方法
EP2687595B1 (en) * 2012-07-19 2018-05-30 Laboratoire Français du Fractionnement et des Biotechnologies Method for purifying transgenic factor VII
US10285915B2 (en) 2012-10-17 2019-05-14 The Procter & Gamble Company Strip for the delivery of an oral care active and methods for applying oral care actives
EP3560946B1 (en) * 2016-12-22 2022-02-02 KM Biologics Co., Ltd. Chromatographic method for collecting blood coagulation factor vii with high yield

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363126A2 (en) * 1988-10-04 1990-04-11 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363126A2 (en) * 1988-10-04 1990-04-11 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins

Also Published As

Publication number Publication date
HU9502846D0 (en) 1995-12-28
CZ253395A3 (en) 1996-01-17
US5700914A (en) 1997-12-23
CA2159313A1 (en) 1994-10-13
WO1994022905A1 (en) 1994-10-13
EP0691984A1 (en) 1996-01-17
AU6423994A (en) 1994-10-24
HUT72712A (en) 1996-05-28
FI954649A0 (fi) 1995-09-29
ZA941956B (en) 1994-09-30
FI954649L (fi) 1995-09-29
NO953883L (no) 1995-11-28
TW278079B (OSRAM) 1996-06-11
AU677309B2 (en) 1997-04-17
PL310887A1 (en) 1996-01-08
IL109164A0 (en) 1994-06-24
DK38293D0 (da) 1993-03-31
FI954649A7 (fi) 1995-09-29
NO953883D0 (no) 1995-09-29
CN1121723A (zh) 1996-05-01
JPH08508264A (ja) 1996-09-03

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