CN103918430A - Styrax hypoglauca cuttage propagation method - Google Patents

Abstract

The invention relates to a cutting propagation method of a white flower tree, relating to a cutting method. At present, the survival rate of the cuttings of the white flower tree is low, which affects the mass propagation of the high-quality seedlings of the white flower tree. The present invention comprises the following steps: 1) Scion collection and processing: after falling leaves and before germination, take 1-year-old seedlings with branches above 0.2m above the ground, cut off side branches and only keep the main trunk, and cut it to 0.4-0.5m long depending on the length of the main trunk For short branches, seal both ends of the short branches with wax; 2) Spike cold storage: After bundling multiple spikes, wrap them with absorbent cotton, spray water with a sprayer to moisten the absorbent cotton, and then put the outer wrapped absorbent cotton into Seal it in a plastic bag and store it in a low-temperature storage cabinet at 2-5°C for later use; 3) Seedbed preparation; 4) Cutting cuttings; 5) Management measures. The cutting survival rate of the technical scheme is high, which can reach 70%, and the cost is low, easy to operate, and can realize mass propagation of high-quality clone seedlings of the white flower tree.

Description

A kind of cutting propagation method of white flower tree

technical field

The invention relates to a cutting method.

Background technique

White flower tree (Styrax tonkinensis) belongs to Styracaceae (Styracaceae) deciduous trees of the genus Styrax. It is a positive tree species. It is naturally distributed in South my country and Southwest China and extends to Vietnam and Laos. temperature zone. It grows rapidly and has a short maturity period, making it an ideal tree species for ultra-short rotation period pulpwood; the seed oil is mainly unsaturated fatty acid, with a total content of 85.4%, and can produce biodiesel; the tree shape is beautiful, the flowers are white in clusters, and it is in full bloom When the flowers are like snow, it is of great ornamental value; its resin is called "benzoin", which has important medicinal value. As a tree species with multiple values such as timber, oil, medicine and ornamental, white flower tree has been increasingly studied at home and abroad in recent years. In terms of reproduction and cultivation, technical problems such as seed seedling cultivation and plantation cultivation have been solved, but asexual reproduction technology has not yet achieved a breakthrough. Asexual reproduction can maintain the excellent traits of the parents, and plays a key and basic role in tree breeding and genetic improvement. Cutting, as a low-cost and highly operable vegetative propagation technique, has not been systematically studied and proposed, which to some extent affects the mass propagation of high-quality seedlings of D. sinensis.

Contents of the invention

The technical problem to be solved and the technical task proposed by the present invention are to perfect and improve the existing technical solutions, and to provide a cutting propagation method of the white flower tree, so as to achieve the purpose of improving the success rate of cutting. For this reason, the present invention takes the following technical solutions.

A method for propagation by cuttings of white flower tree, is characterized in that comprising the following steps:

1) Collection and treatment of cuttings: After falling leaves and before sprouting, take the branches of 1-year-old seedlings that are more than 0.2m above the ground, cut off the side branches and only keep the main trunk, and cut them into short branches with a length of 0.4-0.5m depending on the length of the main trunk. Both ends of the branch are sealed with wax;

2) Cold storage of cuttings: After bundling multiple cuttings, wrap them in absorbent cotton, spray water with a sprayer to moisten the absorbent cotton, then put the cuttings wrapped in absorbent cotton into a plastic bag and seal them, and place them in a low-temperature storage cabinet at 2-5°C storage in reserve;

3) Seedbed preparation: take the cement pool as the cutting seedbed, lay perlite with a thickness greater than 15cm as the substrate, set up an arch shed, cover the arch shed with a film, and evenly spray and disinfect with potassium permanganate solution before cutting;

4) Cutting cuttings: In mid-April, when the average temperature reaches above 15°C, take out the stored cuttings and cut them into cuttings with a length of 10cm-12cm and more than one bud; Cut, and cut obliquely at the lower end; tie several cuttings into a group, put them in the prepared mixed solution containing GGR6 and IBA and soak for 4-8 hours, and the soaking depth is 4-5cm; before cutting, use a thicker than cuttings Punch holes in the substrate with bamboo sticks, the depth of the holes is 8-10cm, and the density is 10cm×10cm or more; put the cuttings in and compact the substrate, spray water until the substrate is saturated after inserting, and then cover the shed;

5) Management measures: After cutting, keep the corresponding humidity of the substrate. When the temperature reaches 30°C, open the two ends of the shed for ventilation and heat dissipation; transplant when more fibrous roots grow.

Cuttings are carried out in mid-April. At this time, the temperature is high, the cell activity is strong, the callus and root system formation time can be effectively shortened, and the cuttings mildew and rot caused by unfavorable factors in the management process can be reduced. Remove the cuttings before they germinate and put them in cold storage to make them dormant.

As a further improvement and supplement to the above technical solutions, the present invention also includes the following additional technical features.

The mass percentage concentrations of GGR6 and IBA in the mixed solution were 300 mg·L ^-1 and 100 mg·L ^-1 , respectively. Both GGR6 and IBA were mainly used for rooting; the mixture of both increased the rooting rate of cuttings.

Cuttings with a diameter of 0.41 to 1.0 cm were used for cuttings.

The cuttings were soaked in the mixed solution for 8 hours.

In mid-February, take 1-year-old seedling branches. Reduce refrigeration time, increase activity, and at the same time, reduce refrigeration costs.

Beneficial effects: the technical solution fills the technical blank of the cutting propagation of the white flower tree, the cutting survival rate is high, which can reach 70%, and the cost is low, easy to operate, and can realize the mass propagation of the high-quality clone seedlings of the white flower tree.

Detailed ways

The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings.

Embodiment one:

This technical solution comprises the following steps:

Scion collection and processing: in mid-February, before the germination of one-year-old seedlings, collect the branches above 0.2m above the ground, cut off the side branches and only keep the main trunk, then cut it to 0.4-0.5m long depending on the length of the main trunk, and seal the two ends with wax ;

Cold storage of cuttings: Wrap the wax-sealed cuttings with moist absorbent cotton, then put them in a sealed plastic bag, and store them in a low-temperature storage cabinet at 2-5°C for later use;

Cutting time: choose fine weather in mid-April;

Seedbed preparation: use a cement pool with a width of 1m and a height of 25cm as the seedbed for cuttings. The bed is covered with perlite with a thickness of 20cm as the substrate, and small arches are built at a distance of 1m and covered with plastic film. Spray and disinfect with potassium permanganate solution evenly before cutting;

Preparation and processing of cuttings: Take out the stored cuttings and cut them into cuttings with a length of about 10 cm, more than one bud, and a diameter of 0.2 to 0.4 cm. The buds are located in the upper part of the cuttings, the top of the cuttings is cut horizontally, and the lower end is cut obliquely. A mixed solution is prepared, and the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 50 mg·L ^-1 respectively. Bundle the prepared cuttings into a group, put them in the prepared mixed solution and soak them for 4 hours at a depth of 4-5cm. Before cutting, use a bamboo stick slightly thicker than the cuttings to punch holes in the substrate. The depth of the holes is 8-10cm, and the density is 10cm×10cm. After putting in the cuttings, compact them, spray water until the substrate is saturated, and then cover the shed; management measures: observe every day after cuttings, spray water appropriately to moisturize, and open both ends of the shed to ventilate and dissipate heat when the temperature reaches 30°C. Transplant when more fibrous roots have grown.

Embodiment 2: the same place as Embodiment 1 will not be repeated, the difference is: the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 100 mg·L ^-1 respectively; the soaking time in the mixed solution is 6 hours.

Embodiment 3: The same parts as in Embodiment 1 will not be repeated, but the difference is that the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 150 mg·L ^-1 respectively, and the soaking time in the mixed solution is 8 hours.

Embodiment 4: The same parts as in Embodiment 1 will not be repeated, but the difference is that cuttings with a diameter of 0.41-1.0 cm are selected, and the soaking time of the cuttings in the mixed solution is 6 hours.

Embodiment 5: The same parts as in Embodiment 1 will not be repeated, the difference is: choose cuttings with a diameter of 0.41 to 1.0 cm, and the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 100 mg·L -1 ^{respectively 1} ; The immersion time in the mixed solution is 8 hours.

Example 6: The same parts as in Example 1 will not be repeated, the difference is: choose cuttings with a diameter of 0.41 to 1.0 cm, and the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 150 mg·L ^{-1 respectively 1.} The immersion time in the mixture is 4 hours.

Embodiment 7: The same place as Embodiment 1 will not be repeated, the difference is: choose cuttings with a diameter greater than 1.0 cm, and the soaking time of the cuttings in the mixed solution is 8 hours.

Embodiment 8: The same parts as in Embodiment 1 will not be repeated, and the difference is: choose cuttings with a diameter greater than 1.0 cm, and the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 100 mg·L ^-1 respectively; The immersion time in the mixture is 8 hours.

Embodiment 9: The same parts as in Embodiment 1 will not be repeated, the difference is: choose cuttings with a diameter greater than 1.0 cm, and the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 150 mg·L ^-1 respectively; The immersion time in the mixture is 6 hours.

The cutting test processing and result analysis of embodiments 1 to 9 are shown in the following table:

As can be seen from the results in the table above, when Example 5 is used, the survival rate of the cuttings of the white flower tree is greatly improved, and the survival rate of the cuttings can reach more than 70%.

A cutting propagation method of white flower tree is a specific embodiment of the present invention, which has already reflected the substantive features and progress of the present invention, and can be equivalent in terms of shape, structure, etc. according to actual use needs under the inspiration of the present invention. All modifications are within the scope of protection of this program.

Claims (5)

1. a method for propagating by cuttings of P. japonica, characterized in that it may further comprise the steps: 1) Collection and treatment of cuttings: After falling leaves and before sprouting, take the branches of 1-year-old seedlings that are more than 0.2m above the ground, cut off the side branches and only keep the main trunk, and cut them into short branches with a length of 0.4-0.5m depending on the length of the main trunk. Both ends of the branch are sealed with wax; 2) Cold storage of cuttings: After bundling multiple cuttings, wrap them in absorbent cotton, spray water with a sprayer to moisten the absorbent cotton, then put the cuttings wrapped in absorbent cotton into a plastic bag and seal them, and place them in a low-temperature storage cabinet at 2-5°C storage in reserve; 3) Seedbed preparation: take the cement pool as the cutting seedbed, lay perlite with a thickness greater than 15cm as the substrate, set up an arch shed, cover the arch shed with a film, and evenly spray and disinfect with potassium permanganate solution before cutting; 4) Cutting cuttings: In mid-April, when the average temperature reaches above 15°C, take out the stored cuttings and cut them into cuttings with a length of 10cm-12cm and more than one bud; Cut, and cut obliquely at the lower end; tie several cuttings into a group, put them in the prepared mixed solution containing GGR6 and IBA and soak for 4-8 hours, and the soaking depth is 4-5cm; before cutting, use a thicker than cuttings Punch holes in the substrate with bamboo sticks, the depth of the holes is 8-10cm, and the density is 10cm×10cm or more; put the cuttings in and compact the substrate, spray water until the substrate is saturated after inserting, and then cover the shed; 5) Management measures: After cutting, keep the corresponding humidity of the substrate. When the temperature reaches 30°C, open the two ends of the shed for ventilation and heat dissipation; transplant when more fibrous roots grow. 2 . The method for propagation by cuttings of D. japonica according to claim 1 , characterized in that: the mass percentage concentrations of GGR6 and IBA in the mixed solution are 300 mg·L ^-1 and 100 mg·L ^-1 , respectively. 3. A method for propagating by cuttings of the white flower tree according to claim 2, characterized in that: cuttings with a diameter of 0.41 to 1.0 cm are used for cuttings. 4. a kind of cutting propagation method of D. japonica according to claim 3, is characterized in that: the cuttings are soaked in the mixed solution for 8 hours. 5. A method for propagating by cuttings of the white flower tree according to claim 1, characterized in that: take 1-year-old seedling branches in the middle and late February.