CN103760355B - 微阵列芯片检测中核苷酸序列的颗粒标记方法 - Google Patents
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- 230000003287 optical effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 229940113082 thymine Drugs 0.000 description 1
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Abstract
Description
突变位点 | 引物名称 | 引物序列(5'->3') |
c.35delG | t35delG-WT | Tag1-TGTTTGTTCACACCCCCGAG |
t35delG-MU | Tag2-TGTTTGTTCACACCCGCAG | |
35delG-RB | Biotin-GCATGCTTGCTTACCCAGAC | |
c.176_191del16 | t176_191del16-WT | Tag3-CCAGGCTGCAAGAACGTGTG |
t176_191del16-MU | Tag4-ACCCTGCAGCCAGCTACG | |
176_191del16-RB | Biotin-GAGCCTTCGATGCGGACC | |
c.235delC | t235delC-WT | Tag5-AAACGGCTATGGGCCCTG |
t235delC-MU | Tag6-ATCCGGCTATGGGCCTG | |
235delC-RB | Biotin-GAGCCTTCGATGCGGACC | |
c.299_300delAT | t299-300delAT-WT | Tag7-TGGCCTACCGGAGACATGA |
t299-300delAT-MU | Tag8-CGTGGCCTACCGGAGACGA | |
299-300delAT-RB | Biotin-GAGCCTTCGATGCGGACC | |
c.2168A>G | t2168A>G-WT | Tag9-GACACATTCTTTATGACGGTCCA |
t2168A>G-MU | Tag10-ACATTCTTTTTGTCGGTCCG | |
2168A>G-RB | Biotin-CAAGGTTTTCCAGATTGCTGAG | |
m.1494C>T | t1494C>T-WT | Tag11-CTTTGAAAGTATACTTGAGGAGG |
t1494C>T-MU | Tag12-CTTTGAAGTATACTTGAGGAGA | |
1494C>T-RB | Biotin-CCCTGATGAAGGCTACAAAG |
突变位点 | 引物名称 | 引物序列(5’->3’) |
c.35delG | t35delG-WT | Tag1-TGTTTGTTCACACCCCCGAG |
t35delG-MU | Tag2-TGTTTGTTCACACCCGCAG | |
35delG-RB | Biotin-GCAT*GCTTGCTTACCCAGAC |
序列名称 | 序列(5'->3') |
SP1 | Biotin-*GCACGCTATCACGTTAGAC |
序列名称 | 序列(5'->3') |
C1494 | ACT*TACCATGTTACGACTAGT |
Claims (8)
Priority Applications (5)
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CN201310651906.5A CN103760355B (zh) | 2013-12-05 | 2013-12-05 | 微阵列芯片检测中核苷酸序列的颗粒标记方法 |
PCT/CN2014/001085 WO2015081612A1 (en) | 2013-12-05 | 2014-12-02 | Luminophore-labeled molecules coupled with particles for microarray-based assays |
US15/100,970 US10167499B2 (en) | 2013-12-05 | 2014-12-02 | Luminophore-labeled molecules coupled with particles for microarray-based assays |
EP14867063.1A EP3077824A4 (en) | 2013-12-05 | 2014-12-02 | Luminophore-labeled molecules coupled with particles for microarray-based assays |
US16/197,318 US10982267B2 (en) | 2013-12-05 | 2018-11-20 | Luminophore-labeled molecules coupled with particles for microarray-based assays |
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CN201310651906.5A CN103760355B (zh) | 2013-12-05 | 2013-12-05 | 微阵列芯片检测中核苷酸序列的颗粒标记方法 |
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US (2) | US10167499B2 (zh) |
EP (1) | EP3077824A4 (zh) |
CN (1) | CN103760355B (zh) |
WO (1) | WO2015081612A1 (zh) |
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EP2601307A4 (en) | 2010-08-06 | 2014-01-01 | Capitalbio Corp | MICROARRAY TEST WITH PARTICLES FOR THE ANALYSIS OF MOLECULAR INTERACTIONS |
CN103760355B (zh) | 2013-12-05 | 2015-09-16 | 博奥生物集团有限公司 | 微阵列芯片检测中核苷酸序列的颗粒标记方法 |
ES2702432B2 (es) | 2017-08-31 | 2019-08-05 | Venegas Pedro Manuel Medina | Método y dispositivo para el análisis de ácidos nucleicos |
CN108048916A (zh) * | 2017-12-20 | 2018-05-18 | 栾图 | 用于核酸富集提取的基因芯片及其制备方法 |
CN110117646A (zh) * | 2018-02-05 | 2019-08-13 | 北京博奥晶典生物技术有限公司 | 一种检测23项遗传性耳聋基因突变的方法 |
CN110184337A (zh) * | 2019-05-16 | 2019-08-30 | 南京诺禾致源生物科技有限公司 | 探针组合物、包含其的试剂、试剂盒、检测方法及应用 |
CN112538519B (zh) * | 2019-09-20 | 2022-09-27 | 华中农业大学 | 一种酶促低场核磁共振dna传感器检测食源性致病菌的方法 |
CN111549181B (zh) * | 2020-05-20 | 2023-08-25 | 广西壮族自治区兽医研究所 | 一种检测禽腺病毒4型的纳米pcr方法、试剂盒及应用 |
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US10982267B2 (en) | 2021-04-20 |
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US20190185922A1 (en) | 2019-06-20 |
US10167499B2 (en) | 2019-01-01 |
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