CN103651395A

CN103651395A - Sterilization composition

Info

Publication number: CN103651395A
Application number: CN201310693583.6A
Authority: CN
Inventors: 刘晓娇; 杨会营; 惠浩浩; 张�浩; 吴勇
Original assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Current assignee: Yanhua Yongle (Leting) Biological Technology Co. Ltd.
Priority date: 2013-12-17
Filing date: 2013-12-17
Publication date: 2014-03-26
Anticipated expiration: 2033-12-17
Also published as: CN103651395B

Abstract

The invention belongs to the technical field of pesticides, and relates to sterilization composition. The sterilization composition is characterized in that active ingredients comprise Jiean-peptide and difenoconazole. The composition can be prepared into emulsion in water and used for control of wheat sharp eyespot, and is wide in fungicide spectrum and environment-friendly.

Description

Bactericidal composition

Technical field the invention belongs to technical field of pesticide, contains specifically the bactericidal composition with synergistic effect of Jiean-peptide and Difenoconazole.

Background technology

The metabolite that Jiean-peptide (jiean-peptide is called for short JAA) derives from bacillus is a kind of novel biological bactericide that has research and development value and application prospect, has good market prospects.The pathogens such as verticillium dahliae, cotton-wilt fusarium, fusarium graminearum, rhizoctonia cerealis, powdery mildew, eliminating vegetable botrytis and downy mildew, muskmelon phytophthora root rot bacterium are all had to stronger inhibition growth and control efficiency.

Difenoconazole (Difenoconazole), Difenoconazole, trade name generation is high, and in triazole bactericidal agent, safety is higher, be widely used in the crops such as fruit tree, vegetables, effectively prevent and treat scab, anthrachose of grape, white rot, spot defoliation, powdery mildew, brown spot, banded sclerotial blight etc.Difenoconazole has protection, treatment and systemic activity, is sterol demethylation inhibitor, suppresses the biosynthesis of cell wall sterol, stops the growth of fungi.Fungicidal spectrum is wide, and leaf surface treatment or seed treatment can improve output and the guaranteed quality of crop.

Wheat sharp eyespot (Rhizotonia cerealis vander Hoeven apud.Boerema & Verhoeven) is that China Mai Qu often sends out venereal disease evil.After disease occurs, general underproduction 10%-20%, serious plot is up to more than 50%.After wheat is infected by the withered bacterium of line, at each growing stage, there is the symptoms such as rotten bud, the rotten stem of withered, the colored stalk of sick seedling, withered strain dead ears.The brown stain of rotten bud bud scale, rear bud is withered to rot, and can not be unearthed; Sick seedling is withered occurs in the 3-4 leaf phase, first existing Intermediate grey on the first leaf sheath only, and the scab of surrounding brown, rear withered because can not taking out the young leaves seedling that causes a disease; After the rotten stem jointing of flower stalk, on base portion leaf sheath, form Intermediate grey, edge beige moire shape scab, after merging, basal part of stem is moire flower stalk shape; Withered strain dead ears scab is invaded after stem wall, taupe in the middle of forming, and the subcircular of surrounding brown or oval eye spot, cause the dehydration of stem wall downright bad, and that supply falls short of demand is withered because of nutrient, moisture for last diseased plant, forms withered strain dead ears.

The development difficulty of bactericide new varieties is larger; and at present because the abuse of bactericide causes existing a lot of sterilization component gradually to produce pesticide resistance; and single sterilization component is because mechanism of action is single; all diseases that usually can disposable elimination cover crop, have so just strengthened peasant's labour cost.Our field control effectiveness test shows, composite between bactericide not only can alleviate pesticide resistance, can also improve drug effect.Meanwhile, bactericide compounded development cost, well below the development cost of new varieties, can be alleviated the economic pressures of the new sterilization component of exploitation, can receive good bactericidal effect again simultaneously.To sum up, we are composite by Jiean-peptide and Difenoconazole for the shortcoming of existing bactericide, to strengthening both drug effects, expand fungicidal spectrum, reduce peasant's labour cost.

Summary of the invention

The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the composite bactericidal composition of Jiean-peptide and Difenoconazole.

Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make aqueous emulsion of the present invention.

In above-mentioned bactericidal composition, the proportion of Jiean-peptide and Difenoconazole is 1:100-50:1.

Above-mentioned bactericidal composition has following characteristics: 1. formulation environmental protection, use less organic principle, and environmentally safe; 2. two kinds of compositions are composite, have synergistic effect; 3. twenty percent divide between without cross resistance; 4. delay the generation of active ingredient resistance, reduce peasant's labour cost.

Embodiment

For determining Jiean-peptide and two kinds of composite synergistic effects of active ingredient of Difenoconazole, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:

First analyzed after the effect feature of two kinds of active ingredients, we are decided to be wheat sharp eyespot (without condition Rhizoctonia cerealis Vander Hoeven) by the raw object of surveying, this fungi Deuteromycotina claims Rhizoctonia cerealis, and this bacterium can cause wheat sharp eyespot.

The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of wheat sharp eyespot mycelia.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the former medicine of 95% Jiean-peptide and 95% difenoconazole technical material be take acetone as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of same amount acetone; Card punch (Φ 5mm) picking rhizoctonia cerealis cake with sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be placed in interior cultivation of constant incubator of 25 ℃, cultivate after 6 days and adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen ₅₀, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Above-mentioned raw survey experiment former medicine used and reagent are all bought from other producers.

The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:

The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).

It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.

Indoor biometrics test data sees the following form:

Table 1 Jiean-peptide, Difenoconazole and the measurement result of different proportioning to wheat sharp eyespot co-toxicity coefficient thereof

Analyze above-mentioned data, can obviously find out when Jiean-peptide and Difenoconazole are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 130, the prevention and control capability of wheat sharp eyespot is had to stronger synergistic effect, wherein Jiean-peptide and Difenoconazole are being that synergy between proportioning five and proportioning seven is significantly visible between 1:5-5:1, the composite desirable medicament of preventing and treating wheat sharp eyespot that becomes of Jiean-peptide and Difenoconazole.

By indoor biometrics, test and determine Jiean-peptide and two kinds of active ingredients of Difenoconazole are composite can good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 20.2% Jiean-peptide difenoconazole water emulsion

Example two, 25.5% Jiean-peptide difenoconazole water emulsion

Example three, 10.5% Jiean-peptide difenoconazole water emulsion

Example four, 22% Jiean-peptide difenoconazole water emulsion

Example five, 24% Jiean-peptide difenoconazole water emulsion

Example six, 30% Jiean-peptide difenoconazole water emulsion

Example seven, 12% Jiean-peptide difenoconazole water emulsion

Example eight, 22% Jiean-peptide difenoconazole water emulsion

Example nine, 13% Jiean-peptide difenoconazole water emulsion

Example ten, 25.5% Jiean-peptide difenoconazole water emulsion

Jiean-peptide 25%

Select above ten field trials that example is prevented and treated wheat sharp eyespot, to determine the effect of formula control wheat sharp eyespot; Simultaneously by the Contrast on effect with 20% Jiean-peptide SC and 10% Difenoconazole WDG, verify the synergistic effect after composite.Concrete test method data are as follows:

30% Jiean-peptide difenoconazole water emulsion and other preparation are done the test of pesticide effectiveness of field control wheat sharp eyespot.Test site is Yangling Shaanxi green house of vegetables district, and the time is on April 12nd, 2012, test each and process three repetitions, and community randomized arrangement, each community area is 12m ², adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of 2 weeks thereafter, specific as follows:

Table 2 duration of test meteorological data

Date	Weather	Have or not special weather situation
			4-12	3-20 ℃ cloudy turn to fine	Nothing
4-13	6-20 ℃ cloudy turn to fine	Nothing
			4-14	8-23 ℃ cloudy turn to fine	Nothing
4-15	11-24 ℃ fine	Nothing
			4-16	12-20 ℃ cloudy turn to light rain	Nothing
4-17	12-23 ℃ cloudy turn to light rain	Nothing
			4-18	10-25 ℃ cloudy	Nothing
4-19	12-22 ℃ cloudy	Nothing
			4-20	11-22 ℃ cloudy	Nothing
4-21	9-28 ℃ fine	Nothing
			4-22	11-28 ℃ fine	Nothing
4-23	The 13-22 ℃ of cloudy thunder shower that turns	Nothing
			4-24	8-22 ℃ light rain to cloudy	Nothing
4-25	10-26 ℃ fine	Nothing
			4-26	11-28 ℃ fine	Nothing

Investigation method is: each community random searching 4 point, and whole blades of every some investigation 2 strains, every leaf accounts for leaf area percentage classification record by lesion area.Investigation in the 7th day after dispenser, dispenser is 2 times altogether, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.

Blade grade scale:

0 grade: without scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for 6%～10% of whole leaf area;

5 grades: lesion area accounts for 11%～20% of whole leaf area;

7 grades: lesion area accounts for 21%～40% of whole leaf area;

9 grades: lesion area accounts for the more than 40% of whole leaf area.

Table 3 30% Jiean-peptide difenoconazole water emulsion and the preventive effect of different medicament to wheat sharp eyespot

From above-mentioned data, can find out, Jiean-peptide and Difenoconazole complex preparation after medicine for the first time 7 days preventive effects all over 76%, and 20% Jiean-peptide SC, 7 days preventive effects after medicine are only 59.44%, the preventive effect of 10% Difenoconazole WDG is 58.37%, compare Jiean-peptide and Difenoconazole single dose, the preventive effect that has utmost point significant difference between complex preparation and single dose, visible two active ingredients are composite good synergistic effect.And after medicine 14 days, all complex preparations have all surpassed 82% to the preventive effect of wheat sharp eyespot.With after medicine, within 7 days, compare for the first time, preventive effect is higher.In summary, complex preparation can well improve drug effect, extends the lasting period, alleviates peasant's the amount of labour, has very high practicality.

Claims

1. a bactericidal composition, is characterized in that: active component is comprised of Jiean-peptide and Difenoconazole, and the weight ratio of described Jiean-peptide and Difenoconazole is 1:100～50:1.

2. bactericidal composition according to claim 1, is characterized in that: by active ingredient and auxiliary material, make aqueous emulsion.

3. according to claim 1 or bactericidal composition claimed in claim 2, it is characterized in that: for preventing and treating wheat sharp eyespot.