CN105794844A

CN105794844A - Organic sterilization composition

Info

Publication number: CN105794844A
Application number: CN201410848986.8A
Authority: CN
Inventors: 高秉全
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-12-31
Filing date: 2014-12-31
Publication date: 2016-07-27

Abstract

The invention discloses an organic sterilization composition. The organic sterilization composition comprises bacillus subtilis, difenoconazole, humus and a dispersant. The organic sterilization composition is characterized by comprising 30-40% of bacillus subtilis, 50-40% of difenoconazole, 20-40% of humus, 5-10% of a dispersant and 5-10% of an emulsion in water. The organic sterilization composition solves the technical problem that the existing sterilization composition has low activity and a poor dispersive capacity in soil. The organic sterilization composition utilizes a complex dispersant to improve difenoconazole dispersibility so that the drug can be fast diffused to disease positions so that wheat sharp eyespot is timely prevented and treated and a loss is reduced.

Description

A kind of organic bactericide compositions

Technical field

The present invention relates to a kind of organic bactericide compositions being made up of bacillus subtilis, Difenoconazole and dispersant, belong to technical field of pesticide, specifically a kind of organic bactericide compositions.

Background technology

Bacillus subtilis, is the one of bacillus.Individual cells 0.7～0.8 × 2～3 μm, uniform coloring, without pod membrane, peritrichous, can move.The conditioned pathogen of pathogenic bacterium or autogenous infection is had obvious inhibitory action by subtilin that its thalline produces in growth course, polymyxin, nystatin, Gramicidin isoreactivity material；Can also producing the anti-fungus polypeptide of a kind of Jiean-peptide by name, multiple fungal disease is had good prevention effect by this composition.Bacterium colony rough surface is opaque, dirty white or slightly yellow, and available protein, multiple sugar and starch decompose tryptophan and forms indole.It is widely distributed in the Organic substance of soil and corruption, easily soaks breeding in juice hay.

Difenoconazole is the antibacterial of a kind of broad-spectrum high efficacy, is also that in triazole bactericidal agent, safety is higher, by suppressing the biosynthesis of pathogenic bacteria cell ergosterol, thus destroying membrane structure and function, stops the growth of fungus.It is widely used in fruit tree, vegetable, Semen Tritici aestivi, Rhizoma Solani tuber osi, beans, the crop such as melon, the multiple fungal disease such as vegetable and melon and fruit is had well protection and therapeutical effect.

Wheat sharp eyespot is a kind of worldwide disease, occurs very general.In recent years, this disease has become China's wheat district regular incidence evil.After Semen Tritici aestivi is infected by the withered bacterium of stricture of vagina, the symptoms such as rotten bud, sick Seedling withered, the flower rotten stem of stalk, withered strain dead ears occur at each growing stage.Rotten bud bud scale brown stain, rear bud is withered to rot, it is impossible to be unearthed；The sick withered generation of Seedling in 3～4 leaf phases, just only existing Intermediate grey on the first sheath, the scab of surrounding brown, after the Seedling that causes a disease because can not taking out young leaves withered；Forming Intermediate grey, the beige moire shape scab in edge after the flower rotten stem jointing of stalk on base portion sheath, after scab merges, basal part of stem is moire flower stalk shape；Withered strain dead ears scab invades after stem wall, taupe in the middle of being formed, the subcircular of surrounding brown or oval eye speckle, causes stem wall dehydration downright bad, last diseased plant because of nutrient, moisture supply falls short of demand and withered, form withered strain dead ears.Additionally, sometimes this disease also can be formed disease invade boundary unconspicuous brown scab.

At present, due to kind, cropping system, fertilizer and water condition change, disease increases the weight of year by year, and lesion constantly expands from south to north.The morbidity underproduction 20～40% early, withered strain dead ears of serious formation or No kernels or seeds are gathered, as in a year of scarcity.In order to solve this problem, patent CN103651395A discloses the technology being entitled as " bactericidal composition ", disclose " Jiean-peptide and the composite bactericidal composition of Difenoconazole ", composite by both compositions not only alleviates Drug resistance, also improve drug effect, and this medicine cost price is relatively low, has good bactericidal effect.But the mobile activity that Difenoconazole is in soil is low, and dispersibility is poor, causing that peasant's dispenser difficulty is big, labour's intensity is big, limits the application of this compound sterilizing thing.

Summary of the invention

It is an object of the invention to: the problem overcoming existing combination sterilization composition dispersibility difference, a kind of organic bactericide compositions is provided, by composite dispersant to improve the problem of Difenoconazole bad dispersibility, reach drug effect dispersibility good, dispenser purpose easily and fast.

To achieve these goals, the present invention adopts the following technical scheme that

A kind of organic bactericide compositions, it is made up of bacillus subtilis, Difenoconazole, humus and dispersant, it is characterised in that: the concrete proportioning of each component is: bacillus subtilis 30～40%, Difenoconazole 50～40%, humus 20～40%, dispersant 5～10%, aqueous emulsion 5～10%.

Further, described dispersant is that Disodium oxalate., P78a-non-toxic banana oil combine, and its combination matching is 1～100:50～1.

Further, the auxiliary agent that described aqueous emulsion is known to the skilled person.

As preferably, described a kind of organic bactericide compositions, it is characterised in that: the concrete proportioning of each component is: bacillus subtilis 35%, Difenoconazole 48%, humus 30%, dispersant 8%, aqueous emulsion 10%.

Compared with existing bactericidal composition, it is an advantage of the current invention that:

1, the organic bactericide combination dosage form environmental protection of the present invention, uses organic toxic component less, environmentally safe；

2, the organic bactericide compositions of the present invention is composite by probiotics and Difenoconazole organic bactericide, not only alleviates the insect resistance effect to medicine, also improves drug effect, have potentiation；

3, the organic bactericide compositions of the present invention with the addition of soil dispersing agent in formula; strengthen two kinds of antibacterial activity in soil and diffusivity; disease position can be diffused to after medicine is applied uniformly, rapidly, kill disease, protection and preventing and treating crop normal growth in time.

4, the organic bactericide compositions of the present invention adopts bacillus subtilis to be one of composite bactericide composition, this strain can produce multiple beneficial rhzomorph in growth course, plant growth is better, and its in fertile soil can normally, Fast-propagation, this provides for improved the disease resistance of soil.

5, the organic bactericide compositions of the present invention can delay the generation of effective ingredient resistance, reduces the labour cost of peasant.

Detailed description of the invention

For determining bacillus subtilis and the composite potentiation of two kinds of effective ingredient of Difenoconazole, it is as follows that applicant has carried out indoor biometrics test, test method and result first by both compositions:

First, after analyzing the action character of two kinds of effective ingredient, the raw object surveyed is decided to be wheat sharp eyespot by us, and this fungi Deuteromycotina claims Rhizoctonia cerealis, and this bacterium can cause wheat sharp eyespot.

The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action that wheat sharp eyespot mycelia is grown by medicament.On the basis of pilot study, from each medicament, pathogenic bacteria mycelial growth inhibition rate reached 3 concentration of design in 20%～90% scope, the medicinal liquid first for solvent, 40% Bacillus subtilis strain liquid, 50% difenoconazole technical material, 10% dispersant, 10% aqueous emulsion being made into series concentration with acetone is standby, then medicinal liquid is joined in the PDA culture medium melting and being cooled to room temperature in 1% ratio, after fully shaking up, pour in the culture dish (1. 90mm) of sterilizing and make band medicine flat board, often process four times repeat, with add equal amount acetone process for blank；With card punch (1. 5mm) the picking rhizoctonia cerealis cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, it is placed in the sad temperature incubator of 25 DEG C and cultivates, decussation method is adopted to measure colony diameter after cultivating 6 days, calculate each medicament ECso to pathogen, and calculate toxicity index according to the following equation, compare virulence and the bacteriostasis of different agents.Above-mentioned raw survey experiment former medicine used and reagent are all bought from other producers.

The co-toxicity of mixture preparation adopts the co-toxicity coefficient method representation that Sun Yun is abundant:

Mixture preparation actual toxicity index ATI=standard agent ECso/ reagent agent ECso × 100

The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine × in mixture the percentage rate of this pharmaceutically active ingredient).

Theoretical toxicity index TTI × 100 of the co-toxicity coefficient CTC=mixture preparation actual toxicity index ATI/ mixture preparation of mixture preparation

It is potentiation when co-toxicity coefficient CTC is more than 120, is antagonism during less than 80, be addition time between 80～120.

Following table is shown in by indoor biometrics test data:

Table 1 bacillus subtilis, Difenoconazole and the different ratio measurement result to wheat sharp eyespot co-toxicity coefficient thereof.

Analyze above-mentioned data, this it appears that when bacillus subtilis and Difenoconazole are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is above 145, the prevention and control capability of wheat sharp eyespot there is stronger potentiation, wherein, when bacillus subtilis and Difenoconazole with 3:4.8 composite time, potentiation is the most notable, say, that the prevention effect of wheat sharp eyespot is best.

Determine bacillus subtilis by indoor biometrics experiment and two kinds of effective ingredient of Difenoconazole are composite good composite effect.The invention will be further described by the examples below, but is not intended to the interest field of the present invention.

Embodiment 1

A kind of organic bactericide compositions, is made up of bacillus subtilis, Difenoconazole, humus and dispersant, and the concrete proportioning of each component is: bacillus subtilis 30%, Difenoconazole 50%, humus 20%, dispersant 5%, aqueous emulsion 5%.

Wherein, described dispersant is that Disodium oxalate., P78a-non-toxic banana oil combine, and its combination matching is 1～100:50～1.

The auxiliary agent that described aqueous emulsion is known to the skilled person.

Embodiment 2

A kind of organic bactericide compositions, it is characterised in that: the concrete proportioning of each component is: bacillus subtilis 35%, Difenoconazole 48%, humus 30%, dispersant 8%, aqueous emulsion 10%.

Embodiment 3

A kind of organic bactericide compositions,

The concrete proportioning of each component is: bacillus subtilis 40%, Difenoconazole 50%, humus 40%, dispersant 5%, aqueous emulsion 10%.

Claims

1. an organic bactericide compositions, it is made up of bacillus subtilis, Difenoconazole, humus and dispersant, it is characterised in that: the concrete proportioning of each component is: bacillus subtilis 30～40%, Difenoconazole 50～40%, humus 20～40%, dispersant 5～10%, aqueous emulsion 5～10%.

2. a kind of organic bactericide compositions as claimed in claim 1, it is characterised in that: the concrete proportioning of each component is: bacillus subtilis 35%, Difenoconazole 48%, humus 30%, dispersant 8%, aqueous emulsion 10%.

3. organic bactericide compositions as claimed in claim 1 or 2 a kind of, it is characterised in that: described dispersant is that Disodium oxalate., P78a-non-toxic banana oil combine, and its combination matching is 1～100:50～1.