CN103641638A - Sustained-release composite microbial fertilizer and preparation method thereof - Google Patents
Sustained-release composite microbial fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention provides a sustained-release composite microbial fertilizer and a preparation method thereof. The fertilizer comprises a fertilizer core and an envelope wrapping the fertilizer core, wherein the fertilizer core contains polyacrylamide, epsilon-polylysine, ascorbic acid, EDTA (ethylene diamine tetraacetic acid), a composite microbial agent and the balance being organic filler. The fertilizer is stable and efficient, and can be easily absorbed and utilized by crops, and damage and pollution to soil can be effectively avoided.
Description
Technical field
The present invention relates to agricultural fertilizer technical field, particularly relate to a kind of slow release type composite microbiological fertilizer and preparation method thereof.
Background technology
Slow release fertilizer claims again slow-release fertilizer, is the class fertilizer new variety that development in recent years is got up, and this class fertilizer can replace the partial function of organic fertilizer, reduces the loss of nutrient in fertilizer, improves fertilizer efficiency, weakens the pollution of fertilizer to soil.
Slow release fertilizer mainly comprises coated fertilizer and low solubility fertilizer.Coated fertilizer is in order to improve function or the performance of fertilizer, is coated with one deck has the fertilizer that microporous hydrophobic indissoluble film is made at its particle surface, utilizes coating pore size, and chemistry or biological decomposition are controlled nutrient releasing speed.Although coating structure plays certain slow releasing function to the main nutrient of fertile core, has obviously reduced total nutrient content, be difficult to apply in producing high-content formula fertilizer in blending mode; In listed part technology, in coated fertilizer composition, containing the difficult degradation materials such as resin above, there is potentially contaminated to soil in life-time service.Therefore need to improve for the defect of aforesaid method, invent a kind of coated fertilizer ecological safety, consumption is few, film forming good, and slow release effect is obvious, the new technology of application increase yield significantly.
In China, due to the life-time service of chemical fertilizer, fertilizer is not enough for many years, all kinds of nutrients ratio imbalances, cause farmland ecological environment, soil physico-chemical property and soil microflora to be subject to destruction in various degree, affected to a certain extent the safety of agricultural-food.Existing chemical fertilizer can be broken the colony balance in soil micro-ecosystem system conventionally, can further cause on the contrary the wildness harm of pathogenic bacteria.
Biological organic fertilizer nutritive element is comprehensive, can improve soil, improves the soil compaction that uses chemical fertilizer to cause.Improve soil physical and chemical property, strengthen the ability of soil water-retaining, fertilizer conservation, fertilizer.Beneficial microorganism in biological organic fertilizer forms mutual symbiosis Relationship With Proliferation with Soil Microorganism after entering soil, suppressing harmful bacteria grows and is converted into probiotics, interact, mutually promote, play the synergy of colony, probiotics produces a large amount of meta-bolitess in growth and breeding process, impel organic decomposition and inversion, can promote and regulation and control plant growth directly or indirectly for crop provides multiple nutrients and pungent, be a kind of environment-friendly type fertilizer.
Biological organic fertilizer in the market is mainly usingd feces of livestock and poultry and the peat composed of rotten mosses, differentiation coal as organic raw material, single feces of livestock and poultry is because its fermentation maturity is slow, atmospheric pollution heavy, occupied ground is large, time-consuming, be difficult to produce in batches on a large scale, be applied to after soil, if become thoroughly decomposed, not exclusively will cause burning seedling.And the peat composed of rotten mosses and differentiation coal are executed very difficult dissolving in soil, organic matter wherein and humic acids are difficult to by crop utilization, affect fertilizer efficiency.
Summary of the invention
Object of the present invention is exactly to provide a kind of slow release type composite microbiological fertilizer and preparation method thereof for the defect of above-mentioned existence, this fertilizer comprises fertilizer core and is coated on the coating outside fertilizer core, fertilizer core contains polyacrylamide, epsilon-polylysine, xitix, EDTA and complex micro organism fungicide, all the other are organic filler, this fertilizer stability and high efficiency, can effectively avoid the infringement of soil and pollution, be easy to Crop utilization.
A kind of slow release type composite microbiological fertilizer of the present invention and preparation method thereof technical scheme is, this fertilizer comprises fertilizer core and is coated on the coating outside fertilizer core, described fertilizer core and the parts by weight of coated fertilizer layer are: fertilizer core 100-150 part, coated fertilizer layer 2-3 part;
The parts by weight of fertilizer core are: polyacrylamide 15-25 part, epsilon-polylysine 5-10 part, xitix 3-5 part, EDTA3-5 part and complex micro organism fungicide 50-150 part, and all the other are organic filler;
Coated fertilizer layer comprises the component of following weight parts: gelatin 2-5 part, polyvinyl alcohol 1-5 part, urea 20-40, ferrous sulfate 5-25 part, zinc sulfate 5-25 part, ammonium citrate 2-5 part, formaldehyde 0.5-1 part, water 30-55 part.
Described complex micro organism fungicide is yeast, azotobacter chroococcum, lichengermium, subtilis, photosynthetic bacteria, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 10-15 hundred million/g, azotobacter chroococcum 15-20 hundred million/g, lichengermium 15-20 hundred million/g, subtilis 15-20 hundred million/g, photosynthetic bacteria 100-150 hundred million/g, bacillus laterosporus 15-20 hundred million/g, bacillus megaterium 15-20 hundred million/g, colloid bacillus cereus 15-20 hundred million/g.
Described organic filler is for being one or more in amino acid sludge, humic acids, feces of livestock and poultry, active sludge powder.
The preparation method of this slow release type composite microbiological fertilizer, step comprises the preparation of fertilizer core, the preparation of coated fertilizer and coating.
The preparation process of fertilizer core is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, then the fermented liquid of single culture is mixed to ferment obtaining mix bacterium agent;
(2) polyacrylamide, epsilon-polylysine, xitix, EDTA are mixed with organic filler, stir;
(3) use disc granulation technology, the mix bacterium agent spraying obtaining is bonded to particle, and employing obtains fertilizer core lower than 60-70 ℃ of oven drying at low temperature in step (1).
In step (1), the bacterial classification in complex micro organism fungicide carries out single culture fermentation and adopts one or more in beef-protein medium, potato culture, LB substratum.
After the bacterial classification in complex micro organism fungicide being fermented separately respectively in step (1), the fermented liquid of single culture is all inoculated in the mixture of polyacrylamide, epsilon-polylysine, xitix, EDTA and organic filler by the inoculum size of 1-2%, ferment, obtain mix bacterium agent.
The preparation process of coated fertilizer is:
(1) gelatin, polyvinyl alcohol are added to the water to mix and blend and are warming up to 60-70 ℃, then add part urea as properties-correcting agent and formaldehyde, continue to stir 20-40 minute;
(2) add successively ferrous sulfate, zinc sulfate, ammonium citrate, add residue urea as solubility promoter, heat to 50-60 ℃ and stir 1-3 hour, make coated fertilizer.
Coating step is: in coating machine, and the fertilizer slug particle surface by coated fertilizer atomized spray in motion, then through air seasoning, coating material is solidified in urea granules surface and form coating, obtain slow release fertilizer.
This fertilizer can be applicable on the greengrocery crops such as the farm crop such as corn, wheat or tomato, cucumber, rape.
Beneficial effect of the present invention is: the beneficial microorganism of slow release type composite microbiological fertilizer of the present invention forms mutual symbiosis Relationship With Proliferation with Soil Microorganism after entering soil, suppressing harmful bacteria grows and is converted into probiotics, interact, mutually promote, play the synergy of colony, probiotics produces a large amount of meta-bolitess in growth and breeding process, impel organic decomposition and inversion, can be directly or indirectly for crop provides multiple nutrients and pungent, promoting and regulation and control plant growth, is a kind of environment-friendly type fertilizer.The fermentation of mix bacterium agent strengthens the synergy between various floras, can reach very high bacterial classification content after fermentation, can not lose efficacy easily, is applied to after soil, can not cause and burn seedling phenomenon.Other compositions of fertilizer core are environmentally friendly, execute in soil and easily dissolve, and are all easy to by crop utilization, and fertilizer efficiency is high.In addition, this fertilizer formula can effectively be kept soil from packing together, and avoids soil property by fertilizer pollution, also can effectively prevent root disease and pest.
The main component of coated fertilizer is the materials such as gelatin, urea, ferrous sulfate, zinc sulfate, the nutrient that can directly or indirectly absorb for crop, and without pedo relict, ecological safety; Polyvinyl alcohol, as film auxiliary agent, makes coated fertilizer better at immersion condition stability inferior.The preparation method of fertilizer of the present invention, has further guaranteed the stability of this fertilizer structure and fertilizer efficiency, and technique is simple, and cost is low, is applicable to large-scale promotion and uses.
embodiment:
In order to understand better the present invention, with specific examples, describe technical scheme of the present invention in detail below, but the present invention is not limited thereto.
Embodiment 1
The preparation method of this slow release type composite microbiological fertilizer, step comprises the preparation of fertilizer core, the preparation of coated fertilizer and coating.
The preparation process of fertilizer core is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum; Then the fermented liquid of single culture is all inoculated into 20 parts of polyacrylamides, 8 parts of epsilon-polylysines, 4 parts, xitix, EDTA4 part by 1% inoculum size, in all the other mixtures that are organic filler, ferments, obtain mix bacterium agent.
Described complex micro organism fungicide is yeast, azotobacter chroococcum, lichengermium, subtilis, photosynthetic bacteria, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,500,000,000/g, azotobacter chroococcum 2,000,000,000/g, lichengermium 2,000,000,000/g, subtilis 2,000,000,000/g, photosynthetic bacteria 15,000,000,000/g, bacillus laterosporus 2,000,000,000/g, bacillus megaterium 2,000,000,000/g, colloid bacillus cereus 2,000,000,000/g.
(2) polyacrylamide, epsilon-polylysine, xitix, EDTA part and organic filler are mixed, stir;
(3) use disc granulation technology, the mix bacterium agent spraying obtaining in step (1) is bonded to particle, and employing obtains fertilizer core lower than 65 ℃ of oven drying at low temperatures, in fertilizer core, the weight part of each component is: 100 parts of 20 parts of polyacrylamides, 8 parts of epsilon-polylysines, 4 parts, xitix, EDTA4 part and complex micro organism fungicides, all the other are organic filler.Described organic filler is for being amino acid sludge and active sludge powder.
Coated fertilizer layer comprises the component of following weight parts: 3 parts, gelatin, 3 parts of polyvinyl alcohol, urea 30,15 parts, ferrous sulfate, 15 parts, zinc sulfate, 3 parts of ammonium citrates, 0.8 part, formaldehyde, 40 parts, water; Preparation process is:
(1) gelatin, polyvinyl alcohol are added to the water to mix and blend and are warming up to 65 ℃, then add part urea as properties-correcting agent and formaldehyde, continue to stir 30 minutes;
(2) add successively ferrous sulfate, zinc sulfate, ammonium citrate, add residue urea as solubility promoter, heat to 55 ℃ and stir 2 hours, make coated fertilizer.
Coating step is: in coating machine, fertilizer slug particle surface by coated fertilizer atomized spray in motion, again through air seasoning, coating material is solidified in urea granules surface and form coating, obtain slow release fertilizer A, the parts by weight of fertilizer core and coated fertilizer layer are: 120 parts, fertilizer core, 2.5 parts, coated fertilizer layer.
This fertilizer can be applicable on the greengrocery crops such as the farm crop such as corn, wheat or tomato, cucumber, rape.
Embodiment 2
The preparation method of this slow release type composite microbiological fertilizer, step comprises the preparation of fertilizer core, the preparation of coated fertilizer and coating.
The preparation process of fertilizer core is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum; Then the fermented liquid of single culture is all inoculated in the mixture of 15 parts of polyacrylamides, 5 parts of epsilon-polylysines, 3 parts, xitix, EDTA3 part and organic filler by 2% inoculum size, ferments, obtain mix bacterium agent.
Described complex micro organism fungicide is yeast, azotobacter chroococcum, lichengermium, subtilis, photosynthetic bacteria, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,200,000,000/g, azotobacter chroococcum 1,800,000,000/g, lichengermium 1,800,000,000/g, subtilis 1,800,000,000/g, photosynthetic bacteria 10,000,000,000/g, bacillus laterosporus 1,800,000,000/g, bacillus megaterium 1,800,000,000/g, colloid bacillus cereus 1,800,000,000/g.
(2) polyacrylamide, epsilon-polylysine, xitix, EDTA part and organic filler are mixed, stir;
(3) use disc granulation technology, the mix bacterium agent spraying obtaining in step (1) is bonded to particle, and employing obtains fertilizer core lower than 60 ℃ of oven drying at low temperatures, in fertilizer core, the weight part of each component is: 50 parts of 15 parts of polyacrylamides, 5 parts of epsilon-polylysines, 3 parts, xitix, EDTA3 part and complex micro organism fungicides, all the other are organic filler.Described organic filler is for being feces of livestock and poultry.
Coated fertilizer layer comprises the component of following weight parts: 2 parts, gelatin, 1 part of polyvinyl alcohol, urea 20,5 parts, ferrous sulfate, 5 parts, zinc sulfate, 2 parts of ammonium citrates, 0.5 part, formaldehyde, 305 parts, water; Preparation process is:
(1) gelatin, polyvinyl alcohol are added to the water to mix and blend and are warming up to 60 ℃, then add part urea as properties-correcting agent and formaldehyde, continue to stir 20 minutes;
(2) add successively ferrous sulfate, zinc sulfate, ammonium citrate, add residue urea as solubility promoter, heat to 50 ℃ and stir 1 hour, make coated fertilizer.
Coating step is: in coating machine, fertilizer slug particle surface by coated fertilizer atomized spray in motion, again through air seasoning, coating material is solidified in urea granules surface and form coating, obtain slow release fertilizer B, the parts by weight of fertilizer core and coated fertilizer layer are: 100 parts, fertilizer core, 2 parts, coated fertilizer layer.
This fertilizer can be applicable on the greengrocery crops such as the farm crop such as corn, wheat or tomato, cucumber, rape.
Embodiment 3
The preparation method of this slow release type composite microbiological fertilizer, step comprises the preparation of fertilizer core, the preparation of coated fertilizer and coating.
The preparation process of fertilizer core is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum; Then the fermented liquid of single culture is all inoculated in the mixture of 25 parts of polyacrylamides, 10 parts of epsilon-polylysines, 5 parts, xitix, EDTA5 part and organic filler by 1.5% inoculum size, ferments, obtain mix bacterium agent.
Described complex micro organism fungicide is yeast, azotobacter chroococcum, lichengermium, subtilis, photosynthetic bacteria, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,000,000,000/g, azotobacter chroococcum 1,500,000,000/g, lichengermium 1,500,000,000/g, subtilis 1,500,000,000/g, photosynthetic bacteria 13,000,000,000/g, bacillus laterosporus 1,500,000,000/g, bacillus megaterium 1,500,000,000/g, colloid bacillus cereus 1,500,000,000/g.
(2) polyacrylamide, epsilon-polylysine, xitix, EDTA part and organic filler are mixed, stir;
(3) use disc granulation technology, the mix bacterium agent spraying obtaining in step (1) is bonded to particle, and employing obtains fertilizer core lower than 70 ℃ of oven drying at low temperatures, in fertilizer core, the weight part of each component is: 150 parts of 25 parts of polyacrylamides, 10 parts of epsilon-polylysines, 5 parts, xitix, EDTA5 part and complex micro organism fungicides, all the other are organic filler.Described organic filler is for being humic acids.
Coated fertilizer layer comprises the component of following weight parts: 5 parts, gelatin, 5 parts of polyvinyl alcohol, urea 40,25 parts, ferrous sulfate, 25 parts, zinc sulfate, 5 parts of ammonium citrates, 1 part, formaldehyde, 55 parts, water; Preparation process is:
(1) gelatin, polyvinyl alcohol are added to the water to mix and blend and are warming up to 70 ℃, then add part urea as properties-correcting agent and formaldehyde, continue to stir 40 minutes;
(2) add successively ferrous sulfate, zinc sulfate, ammonium citrate, add residue urea as solubility promoter, heat to 60 ℃ and stir 3 hours, make coated fertilizer.
Coating step is: in coating machine, fertilizer slug particle surface by coated fertilizer atomized spray in motion, again through air seasoning, coating material is solidified in urea granules surface and form coating, obtain slow release fertilizer C, the parts by weight of fertilizer core and coated fertilizer layer are: 150 parts, fertilizer core, 3 parts, coated fertilizer layer.
This fertilizer can be applicable on the greengrocery crops such as the farm crop such as corn, wheat or tomato, cucumber, rape.
Embodiment 4
The preparation method of this slow release type composite microbiological fertilizer, step comprises the preparation of fertilizer core, the preparation of coated fertilizer and coating.
The preparation process of fertilizer core is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, single culture fermentation adopts one or more in beef-protein medium, potato culture, LB substratum; Then the fermented liquid of single culture is all inoculated in the mixture of 25 parts of polyacrylamides, 5 parts of epsilon-polylysines, 5 parts, xitix, EDTA3 part and organic filler by 1% inoculum size, ferments, obtain mix bacterium agent.
Described complex micro organism fungicide is yeast, azotobacter chroococcum, lichengermium, subtilis, photosynthetic bacteria, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus.
In described complex micro organism fungicide, the content of each bacterial classification is: yeast 1,500,000,000/g, azotobacter chroococcum 1,800,000,000/g, lichengermium 2,000,000,000/g, subtilis 1,600,000,000/g, photosynthetic bacteria 15,000,000,000/g, bacillus laterosporus 1,800,000,000/g, bacillus megaterium 2,000,000,000/g, colloid bacillus cereus 100,000,000/g.
(2) polyacrylamide, epsilon-polylysine, xitix, EDTA part and organic filler are mixed, stir;
(3) use disc granulation technology, the mix bacterium agent spraying obtaining in step (1) is bonded to particle, and employing obtains fertilizer core lower than 65 ℃ of oven drying at low temperatures, in fertilizer core, the weight part of each component is: 120 parts of 25 parts of polyacrylamides, 5 parts of epsilon-polylysines, 5 parts, xitix, EDTA3 part and complex micro organism fungicides, all the other are organic filler.Described organic filler is the mixture for amino acid sludge, humic acids, feces of livestock and poultry, active sludge powder.
Coated fertilizer layer comprises the component of following weight parts: 4 parts, gelatin, 5 parts of polyvinyl alcohol, urea 25,10 parts, ferrous sulfate, 15 parts, zinc sulfate, 3 parts of ammonium citrates, 1 part, formaldehyde, 45 parts, water; Preparation process is:
(1) gelatin, polyvinyl alcohol are added to the water to mix and blend and are warming up to 65 ℃, then add part urea as properties-correcting agent and formaldehyde, continue to stir 30 minutes;
(2) add successively ferrous sulfate, zinc sulfate, ammonium citrate, add residue urea as solubility promoter, heat to 50 ℃ and stir 2 hours, make coated fertilizer.
Coating step is: in coating machine, fertilizer slug particle surface by coated fertilizer atomized spray in motion, again through air seasoning, coating material is solidified in urea granules surface and form coating, obtain slow release fertilizer D, the parts by weight of fertilizer core and coated fertilizer layer are: 150 parts, fertilizer core, 2 parts, coated fertilizer layer.
This fertilizer can be applicable on the greengrocery crops such as the farm crop such as corn, wheat or tomato, cucumber, rape.
Embodiment 5
Test objective
Test objective is that A, B, C, D fertilizer and general chemistry fertilizer in comparison embodiment of the present invention 1-4 is on the impact of corn growth, effect of increasing production.
Testing program
Process 1, contrast, does not apply fertilizer;
Process 2, blending chemical fertilizer is bought in common market;
Process 3, fertilizer A of the present invention, B, C, D.
Result
Table 1
Embodiment 6
Test objective
Test objective is that A, B, C, D fertilizer and general chemistry fertilizer in comparison embodiment of the present invention 1-4 is on the impact of wheat growth, effect of increasing production.
Testing program
Process 1, contrast, does not apply fertilizer;
Process 2, blending chemical fertilizer is bought in common market;
Process 3, fertilizer A of the present invention, B, C, D.
Result
In the processing 3 of the survey showed that each breeding time fertilizer of the present invention, in winter resistance, drought resistance and maturation, fall aspect Huang than processing 1 and process 2 and do very well.
Table 2
The beneficial microorganism of slow release type composite microbiological fertilizer of the present invention forms mutual symbiosis Relationship With Proliferation with Soil Microorganism after entering soil, suppressing harmful bacteria grows and is converted into probiotics, interact, mutually promote, play the synergy of colony, probiotics produces a large amount of meta-bolitess in growth and breeding process, impel organic decomposition and inversion, can promote and regulation and control plant growth directly or indirectly for crop provides multiple nutrients and pungent, be a kind of environment-friendly type fertilizer.The fermentation of mix bacterium agent strengthens the synergy between various floras, can reach very high bacterial classification content after fermentation, can not lose efficacy easily, is applied to after soil, can not cause and burn seedling phenomenon.Other compositions of fertilizer core are environmentally friendly, execute in soil and easily dissolve, and are all easy to by crop utilization, and fertilizer efficiency is high.In addition, this fertilizer formula can effectively be kept soil from packing together, and avoids soil property by fertilizer pollution, also can effectively prevent root disease and pest.
The main component of coated fertilizer is the materials such as gelatin, urea, ferrous sulfate, zinc sulfate, the nutrient that can directly or indirectly absorb for crop, and without pedo relict, ecological safety; Polyvinyl alcohol, as film auxiliary agent, makes coated fertilizer better at immersion condition stability inferior.The preparation method of fertilizer of the present invention, has further guaranteed the stability of this fertilizer structure and fertilizer efficiency, and technique is simple, and cost is low, is applicable to large-scale promotion and uses.
Claims (10)
1. a slow release type composite microbiological fertilizer, comprises fertilizer core and is coated on the coating outside fertilizer core, and it is characterized in that, described fertilizer core and the parts by weight of coated fertilizer layer are: fertilizer core 100-150 part, coated fertilizer layer 2-3 part;
The parts by weight of fertilizer core are: polyacrylamide 15-25 part, epsilon-polylysine 5-10 part, xitix 3-5 part, EDTA3-5 part and complex micro organism fungicide 50-150 part, and all the other are organic filler;
Coated fertilizer layer comprises the component of following weight parts: gelatin 2-5 part, polyvinyl alcohol 1-5 part, urea 20-40, ferrous sulfate 5-25 part, zinc sulfate 5-25 part, ammonium citrate 2-5 part, formaldehyde 0.5-1 part, water 30-55 part.
2. a kind of slow release type composite microbiological fertilizer according to claim 1, it is characterized in that, described complex micro organism fungicide is yeast, azotobacter chroococcum, lichengermium, subtilis, photosynthetic bacteria, bacillus laterosporus, bacillus megaterium, colloid bacillus cereus.
3. a kind of slow release type composite microbiological fertilizer according to claim 2, it is characterized in that, in described complex micro organism fungicide, the content of each bacterial classification is: yeast 10-15 hundred million/g, azotobacter chroococcum 15-20 hundred million/g, lichengermium 15-20 hundred million/g, subtilis 15-20 hundred million/g, photosynthetic bacteria 100-150 hundred million/g, bacillus laterosporus 15-20 hundred million/g, bacillus megaterium 15-20 hundred million/g, colloid bacillus cereus 15-20 hundred million/g.
4. a kind of slow release type composite microbiological fertilizer according to claim 1, is characterized in that, described organic filler is for being one or more in amino acid sludge, humic acids, feces of livestock and poultry, active sludge powder.
5. the preparation method of a kind of slow release type composite microbiological fertilizer as described in as arbitrary in claim 1-4, is characterized in that, step comprises the preparation of fertilizer core, the preparation of coated fertilizer and coating.
6. the preparation method of a kind of slow release type composite microbiological fertilizer according to claim 5, is characterized in that, the preparation process of fertilizer core is:
(1) obtain respectively the fermented liquid of each bacterial classification after the bacterial classification in complex micro organism fungicide is fermented separately respectively, then the fermented liquid of single culture is mixed to ferment obtaining mix bacterium agent;
(2) polyacrylamide, epsilon-polylysine, xitix, EDTA are mixed with organic filler, stir;
(3) use disc granulation technology, the mix bacterium agent spraying obtaining is bonded to particle, and employing obtains fertilizer core lower than 60-70 ℃ of oven drying at low temperature in step (1).
7. the preparation method of a kind of slow release type composite microbiological fertilizer according to claim 6, it is characterized in that, in step (1), the bacterial classification in complex micro organism fungicide carries out single culture fermentation and adopts one or more in beef-protein medium, potato culture, LB substratum.
8. the preparation method of a kind of slow release type composite microbiological fertilizer according to claim 6, it is characterized in that, after the bacterial classification in complex micro organism fungicide being fermented separately respectively in step (1), the fermented liquid of single culture is all inoculated in the mixture of polyacrylamide, epsilon-polylysine, xitix, EDTA and organic filler by the inoculum size of 1-2%, ferment, obtain mix bacterium agent.
9. the preparation method of a kind of slow release type composite microbiological fertilizer according to claim 5, is characterized in that, the preparation process of coated fertilizer is:
(1) gelatin, polyvinyl alcohol are added to the water to mix and blend and are warming up to 60-70 ℃, then add part urea as properties-correcting agent and formaldehyde, continue to stir 20-40 minute;
(2) add successively ferrous sulfate, zinc sulfate, ammonium citrate, add residue urea as solubility promoter, heat to 50-60 ℃ and stir 1-3 hour, make coated fertilizer.
10. the preparation method of a kind of slow release type composite microbiological fertilizer according to claim 5, it is characterized in that, coating step is: in coating machine, fertilizer slug particle surface by coated fertilizer atomized spray in motion, again through air seasoning, coating material is solidified in urea granules surface and form coating, obtain slow release fertilizer.
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