CN104892151A - Preparation method of sustained-release composite microbial fertilizer for codonopsis pilosula planting - Google Patents
Preparation method of sustained-release composite microbial fertilizer for codonopsis pilosula planting Download PDFInfo
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Abstract
The invention discloses a preparation method of sustained-release composite microbial fertilizer for codonopsis pilosula planting, belonging to the technical field of microbial fertilizer. The preparation method of the sustained-release composite microbial fertilizer for codonopsis pilosula planting comprises the following steps of: mixing paenibacillus polymyxa liquid, ketobutanol clostridium liquid, 10 to 20 parts of bacillus mucilaginosus liquid and bacillus subtilis uniformly to obtain mixed bacterial liquid; mixing polyacrylamide, stearic acid, agar and cow dung to obtain a mixed material; spraying the mixed bacterial liquid on the mixed material to form particles through adhesion, and drying to obtain a fertilizer core; preparing a coated material; in a coating machine, atomizing and spraying the coated material on the surface of the moving fertilizer core particle; and performing aeration drying so as to solidify the coated material on the surface of the fertilizer core to form a coating to obtain the sustained-release fertilizer. The sustained-release fertilizer prepared by the method can be used for effectively promoting the growth of codonopsis pilosula and can increase the content of effective components of the codonopsis pilosula.
Description
Technical field
The present invention discloses a kind of preparation method of the Sustained-release composite microbial fertilizer for Radix Codonopsis plantation, belongs to technical field of biological fertilizer.
Background technology
Radix Codonopsis is traditional tonic that China commonly uses, and the Radix Codonopsis that ancient times produce with area, Shangdang, Shanxi is top grade, has invigorating the spleen and replenishing QI, effect of invigorating the spleen benefit lung.Modern study, Radix Codonopsis, containing various saccharides, phenols, sterol, volatile oil, scutellarein glucosid, saponin and Alkaloid, has strengthening immunity, vasodilation, step-down, improves microcirculation, strengthens the effects such as hemopoietic function.In addition castering action is had to the leukopenia that chemotherapy radiotherapy causes.
Slow release fertilizer changes plant effective state nutrient into rate of release after referring to incorporation of fertilizerin the soil is far smaller than instant fertilizer, can slowly release its nutrient in soil.It has slow or long-lasting to crop, and it can only delay the release rate of fertilizer, does not reach the object of complete controlled release.The advanced form of slow release fertilizer is release and release control fertilizer, and it makes the speed of fertilizer Releasing nutrient and crop need the amount of nutrient consistent, and make utilization rate of fertilizer reach the highest, broadly release and release control fertilizer includes slow release fertilizer.Slow release fertilizer can according to nutritive properties such as crop alimentary stage, continuitys, utilize the means such as physics, chemistry, biology to regulate and nutrient supply intensity and the capacity such as trace element of Controlled-release nitrogen, phosphorus, potassium and necessity, fertilizer emergency can be reached and to help mutually long-acting, the efficient plant nutrition complex body of effect.
Research at present for the slow release fertilizer being specifically designed to Radix Codonopsis plantation is also less, is therefore necessary to develop a kind of slow release fertilizer that can improve Radix Codonopsis output and active constituent content.
Summary of the invention
The object of the invention is: a kind of preparation method being specifically designed to the microbial fertilizer of Radix Codonopsis plantation is provided.
Technical scheme is as follows:
For a preparation method for the Sustained-release composite microbial fertilizer of Radix Codonopsis plantation, comprise the steps:
1st step, by weight, by Paenibacillus polymyxa (
paenilbacillus polymyxa) bacterium liquid 20 ~ 30 parts, ketone Clostridium acetobutylicum (
clostridium acetobutylicum) bacterium liquid 10 ~ 20 parts, bacillusmusilaginosiengineering (
bacillus mucilaginosus) bacterium liquid 10 ~ 20 parts, subtilis (
bacillus subtilis) bacterium liquid 30 ~ 40 parts mixes, and obtains mixed bacteria liquid;
2nd step, polyacrylamide 5 ~ 10 parts, stearic acid 3 ~ 6 parts, 2 ~ 4 parts, agar, cow dung 15 ~ 20 parts mixing, stir, obtain compound;
3rd step, use disc granulation technology, mixed bacteria liquid is sprayed on compound and is bonded to particle, dry and obtain fertilizer core;
4th step, ammonium phosphate-nitrate 0.2 ~ 0.4 part, monoammonium phosphate 0.1 ~ 0.3 part, diammonium phosphate 0.05 ~ 0.1 part, double superhosphate 0.05 ~ 0.1 part, 0.05 ~ 0.1 part, Repone K, potassium sulfate 0.03 ~ 0.06 part, 0.2 ~ 0.4 part, gelatin, polyvinyl alcohol 0.1 ~ 0.3 part, starch 0.5 ~ 0.8 part, wilkinite 0.1 ~ 0.3 part, cellulose acetate 0.05 ~ 0.08 part, 0.03 ~ 0.06 part, Yelkin TTS, 6 ~ 12 parts, water to be mixed, heat and stir, obtained coated fertilizer;
5th step, in coating machine, by the fertilizer slug particle surface of coated fertilizer atomized spray in motion, then through air seasoning, coated fertilizer is solidified and forms coating in fertilizer wicking surface, obtain slow release fertilizer.
In the 1st described step, the bacterial classification content in each bacterium liquid is 10 ~ 1,500,000,000/g.
Bake out temperature in the 3rd described step is 60 ~ 70 DEG C.
In the 4th described step, warm temperature is 50 ~ 60 DEG C, and churning time is 1 ~ 3 hour.
In the 4th described step, the molecular weight ranges of polyvinyl alcohol is 2000 ~ 4000.
beneficial effect
The slow release fertilizer that the present invention prepares can promote the growth of Radix Codonopsis effectively, and can improve the content of effective constituent in Radix Codonopsis.
Embodiment
Embodiment 1
1st step, by Paenibacillus polymyxa (
paenilbacillus polymyxa) bacterium liquid 20Kg, ketone Clostridium acetobutylicum (
clostridium acetobutylicum) bacterium liquid 10Kg, bacillusmusilaginosiengineering (
bacillus mucilaginosus) bacterium liquid 10Kg, subtilis (
bacillus subtilis) bacterium liquid 30Kg mixes, obtain mixed bacteria liquid, the bacterial classification content in each bacterium liquid is 10 ~ 1,500,000,000/g;
2nd step, polyacrylamide 5Kg, stearic acid 3Kg, agar 2Kg, cow dung 15Kg mix, and stir, obtain compound;
3rd step, use disc granulation technology, be sprayed at by mixed bacteria liquid on compound and be bonded to particle, 60 DEG C of oven dry obtain fertilizer core;
4th step, ammonium phosphate-nitrate 0.2Kg, monoammonium phosphate 0.1Kg, diammonium phosphate 0.05Kg, double superhosphate 0.05Kg, Repone K 0.05Kg, potassium sulfate 0.03Kg, gelatin 0.2Kg, polyvinyl alcohol (molecular weight ranges is 2000 ~ 4000) 0.1Kg, starch 0.5Kg, wilkinite 0.1Kg, cellulose acetate 0.05Kg, Yelkin TTS 0.03Kg, water 6Kg to be mixed, heat to 50 DEG C and stir 1 hour, obtained coated fertilizer;
5th step, in coating machine, by the fertilizer slug particle surface of coated fertilizer atomized spray in motion, then through air seasoning, coated fertilizer is solidified and forms coating in fertilizer wicking surface, obtain slow release fertilizer.
Embodiment 2
1st step, by Paenibacillus polymyxa (
paenilbacillus polymyxa) bacterium liquid 30Kg, ketone Clostridium acetobutylicum (
clostridium acetobutylicum) bacterium liquid 20Kg, bacillusmusilaginosiengineering (
bacillus mucilaginosus) bacterium liquid 20Kg, subtilis (
bacillus subtilis) bacterium liquid 40Kg mixes, obtain mixed bacteria liquid, the bacterial classification content in each bacterium liquid is 1,500,000,000/more than g;
2nd step, polyacrylamide 10Kg, stearic acid 6Kg, agar 4Kg, cow dung 20Kg mix, and stir, obtain compound;
3rd step, use disc granulation technology, be sprayed at by mixed bacteria liquid on compound and be bonded to particle, 70 DEG C of oven dry obtain fertilizer core;
4th step, ammonium phosphate-nitrate 0.4Kg, monoammonium phosphate 0.3Kg, diammonium phosphate 0.1Kg, double superhosphate 0.1Kg, Repone K 0.1Kg, potassium sulfate 0.06Kg, gelatin 0.4Kg, polyvinyl alcohol (molecular weight ranges is 2000 ~ 4000) 0.3Kg, starch 0.8Kg, wilkinite 0.3Kg, cellulose acetate 0.08Kg, Yelkin TTS 0.06Kg, water 12Kg to be mixed, heat to 60 DEG C and stir 3 hours, obtained coated fertilizer;
5th step, in coating machine, by the fertilizer slug particle surface of coated fertilizer atomized spray in motion, then through air seasoning, coated fertilizer is solidified and forms coating in fertilizer wicking surface, obtain slow release fertilizer.
Embodiment 3
1st step, by Paenibacillus polymyxa (
paenilbacillus polymyxa) bacterium liquid 25Kg, ketone Clostridium acetobutylicum (
clostridium acetobutylicum) bacterium liquid 15Kg, bacillusmusilaginosiengineering (
bacillus mucilaginosus) bacterium liquid 15Kg, subtilis (
bacillus subtilis) bacterium liquid 35Kg mixes, obtain mixed bacteria liquid, the bacterial classification content in each bacterium liquid is 1,200,000,000/more than g;
2nd step, polyacrylamide 7Kg, stearic acid 5Kg, agar 3Kg, cow dung 18Kg mix, and stir, obtain compound;
3rd step, use disc granulation technology, be sprayed at by mixed bacteria liquid on compound and be bonded to particle, 65 DEG C of oven dry obtain fertilizer core;
4th step, ammonium phosphate-nitrate 0.3Kg, monoammonium phosphate 0.2Kg, diammonium phosphate 0.07Kg, double superhosphate 0.08Kg, Repone K 0.07Kg, potassium sulfate 0.05Kg, gelatin 0.3Kg, polyvinyl alcohol (molecular weight ranges is 2000 ~ 4000) 0.2Kg, starch 0.6Kg, wilkinite 0.2Kg, cellulose acetate 0.06Kg, Yelkin TTS 0.04Kg, water 8Kg to be mixed, heat to 55 DEG C and stir 2 hours, obtained coated fertilizer;
5th step, in coating machine, by the fertilizer slug particle surface of coated fertilizer atomized spray in motion, then through air seasoning, coated fertilizer is solidified and forms coating in fertilizer wicking surface, obtain slow release fertilizer.
Reference examples 1
Be with the difference of embodiment 3: in mixed bacteria liquid, do not add bacillusmusilaginosiengineering.
1st step, by Paenibacillus polymyxa (
paenilbacillus polymyxa) bacterium liquid 25Kg, ketone Clostridium acetobutylicum (
clostridium acetobutylicum) bacterium liquid 15Kg, bacillusmusilaginosiengineering (
bacillus mucilaginosus) bacterium liquid 15Kg mixes, obtain mixed bacteria liquid, the bacterial classification content in each bacterium liquid is 1,200,000,000/more than g;
2nd step, polyacrylamide 7Kg, stearic acid 5Kg, agar 3Kg, cow dung 18Kg mix, and stir, obtain compound;
3rd step, use disc granulation technology, be sprayed at by mixed bacteria liquid on compound and be bonded to particle, 65 DEG C of oven dry obtain fertilizer core;
4th step, ammonium phosphate-nitrate 0.3Kg, monoammonium phosphate 0.2Kg, diammonium phosphate 0.07Kg, double superhosphate 0.08Kg, Repone K 0.07Kg, potassium sulfate 0.05Kg, gelatin 0.3Kg, polyvinyl alcohol (molecular weight ranges is 2000 ~ 4000) 0.2Kg, starch 0.6Kg, wilkinite 0.2Kg, cellulose acetate 0.06Kg, Yelkin TTS 0.04Kg, water 8Kg to be mixed, heat to 55 DEG C and stir 2 hours, obtained coated fertilizer;
5th step, in coating machine, by the fertilizer slug particle surface of coated fertilizer atomized spray in motion, then through air seasoning, coated fertilizer is solidified and forms coating in fertilizer wicking surface, obtain slow release fertilizer.
Reference examples 2
Be with the difference of embodiment 3: in coated fertilizer, do not add Yelkin TTS.
1st step, by Paenibacillus polymyxa (
paenilbacillus polymyxa) bacterium liquid 25Kg, ketone Clostridium acetobutylicum (
clostridium acetobutylicum) bacterium liquid 15Kg, bacillusmusilaginosiengineering (
bacillus mucilaginosus) bacterium liquid 15Kg, subtilis (
bacillus subtilis) bacterium liquid 35Kg mixes, obtain mixed bacteria liquid, the bacterial classification content in each bacterium liquid is 1,200,000,000/more than g;
2nd step, polyacrylamide 7Kg, stearic acid 5Kg, agar 3Kg, cow dung 18Kg mix, and stir, obtain compound;
3rd step, use disc granulation technology, be sprayed at by mixed bacteria liquid on compound and be bonded to particle, 65 DEG C of oven dry obtain fertilizer core;
4th step, ammonium phosphate-nitrate 0.3Kg, monoammonium phosphate 0.2Kg, diammonium phosphate 0.07Kg, double superhosphate 0.08Kg, Repone K 0.07Kg, potassium sulfate 0.05Kg, gelatin 0.3Kg, polyvinyl alcohol (molecular weight ranges is 2000 ~ 4000) 0.2Kg, starch 0.6Kg, wilkinite 0.2Kg, cellulose acetate 0.06Kg, water 8Kg to be mixed, heat to 55 DEG C and stir 2 hours, obtained coated fertilizer;
5th step, in coating machine, by the fertilizer slug particle surface of coated fertilizer atomized spray in motion, then through air seasoning, coated fertilizer is solidified and forms coating in fertilizer wicking surface, obtain slow release fertilizer.
Planting experiment
Radix Codonopsis planting experiment is carried out, greenhouse gardening, each test group area 30m in somewhere, Shanxi
2, spacing in the rows 20 ~ 30cm, every m
2fertilising 2Kg, applies fertilizer from during seedlings plugging in March then, to late October next year seedling, investigates per mu yield in whole seedling raising process, lobetyolin's content.
The detection of lobetyolin's content adopts HPLC method, and step is as follows:
Instrument
Agilent 1200 high performance liquid chromatograph, comprise AgilentC1311A quaternary pump (Quatpump), the online de-aerator of Agilent G1322A vacuum (Degasser), Agilent G1329A automatic sampler (ALS), Agilent C1315D DAD detector, Agilent 1200 chem workstation, Agilent C1316A (TCC) column oven, chromatographic column Agilent Eclipse XDB-C18 (150mm × 4.6mm, 5 μm), AL204 type electronic balance (METTLER TOIEDO).
Reagent
Lobetyolin's reference substance (Nat'l Pharmaceutical & Biological Products Control Institute, lot number 111732-200904), acetonitrile, methyl alcohol are chromatographically pure, and all the other reagent are analytical pure.Radix Codonopsis is Radix Codonopsis, Liaoning Radix Codonopsis, Gansu Radix Codonopsis.
Chromatographic condition
Chromatographic column: Agilent Eclipse XDB-C18 (150mm × 4.6mm, 5 μm); Moving phase: acetonitrile-water (28:72), flow velocity: l.0mL/min; Column temperature: room temperature; Determined wavelength: 267nm.Sample size is l0mL.The theoretical stage tower number of chromatographic column is not less than 5000nm.Be calculated as with this understanding by lobetyolin peak, lobetyolin and other components all can reach baseline separation.
Prepared by reference substance solution
Precision takes lobetyolin reference substance 5.0mg. and is placed in l0mL measuring bottle, adds ethanol solution and is diluted to scale, shaking up and obtain 0.5mg.mL
-1reference substance storing solution.Draw reference substance storing solution 100 μ L by microsyringe precision, put in ImL measuring bottle, add dehydrated alcohol and be diluted to scale, shake up and obtain 0.05 mg.mL
-1reference substance solution.
The preparation of need testing solution
Get Radix Codonopsis control medicinal material appropriate, smash to pieces, cross 40 mesh sieves, powder, in 4 DEG C of dry 5h, is placed in moisture eliminator for subsequent use.Precision takes dry Radix Codonopsis powder 0.5g, puts in measuring bottle, adds dehydrated alcohol l0mL, ultrasonic 25min, and filter residue in measuring bottle and add dehydrated alcohol 8mL again, ultrasonic 15min, filter, residue adds dehydrated alcohol 8mL again, ultrasonic l0min, filters, merges 3 filtrates.Filtrate put water-bath flings to absolute alcohol closely dry after, residue adds methanol solution, and is transferred in 5mL measuring bottle, adds methyl alcohol to scale, shakes up, and with 0.45 μm of millipore filtration, discards just filtrate, collects.
Table 1
Per mu yield kg | Lobetyolin content % | |
Embodiment 1 | 198.1 | 8.34 |
Embodiment 2 | 205.4 | 8.14 |
Embodiment 3 | 213.5 | 8.89 |
Reference examples 1 | 165.4 | 7.87 |
Reference examples 2 | 178.2 | 7.92 |
As can be seen from the table, the slow release fertilizer that the present invention prepares can promote the growth of Radix Codonopsis effectively, and can improve the content of effective constituent in Radix Codonopsis.Wherein, embodiment 3, compared with reference examples 1, by adding bacillusmusilaginosiengineering, can improve lobetyolin's content effectively; Embodiment 3, compared with reference examples 2, effectively can improve slow release effect by adding Yelkin TTS in coated fertilizer, reaching the effect of volume increase.
Claims (5)
1., for a preparation method for the Sustained-release composite microbial fertilizer of Radix Codonopsis plantation, it is characterized in that, comprise the steps:
1st step, by weight, Paenibacillus polymyxa bacterium liquid 20 ~ 30 parts, ketone Clostridium acetobutylicum bacterium liquid 10 ~ 20 parts, bacillusmusilaginosiengineering bacterium liquid 10 ~ 20 parts, subtilis bacterium liquid 30 ~ 40 parts are mixed, obtains mixed bacteria liquid;
2nd step, polyacrylamide 5 ~ 10 parts, stearic acid 3 ~ 6 parts, 2 ~ 4 parts, agar, cow dung 15 ~ 20 parts mixing, stir, obtain compound;
3rd step, use disc granulation technology, mixed bacteria liquid is sprayed on compound and is bonded to particle, dry and obtain fertilizer core;
4th step, ammonium phosphate-nitrate 0.2 ~ 0.4 part, monoammonium phosphate 0.1 ~ 0.3 part, diammonium phosphate 0.05 ~ 0.1 part, double superhosphate 0.05 ~ 0.1 part, 0.05 ~ 0.1 part, Repone K, potassium sulfate 0.03 ~ 0.06 part, 0.2 ~ 0.4 part, gelatin, polyvinyl alcohol 0.1 ~ 0.3 part, starch 0.5 ~ 0.8 part, wilkinite 0.1 ~ 0.3 part, cellulose acetate 0.05 ~ 0.08 part, 0.03 ~ 0.06 part, Yelkin TTS, 6 ~ 12 parts, water to be mixed, heat and stir, obtained coated fertilizer;
5th step, in coating machine, by the fertilizer slug particle surface of coated fertilizer atomized spray in motion, then through air seasoning, coated fertilizer is solidified and forms coating in fertilizer wicking surface, obtain slow release fertilizer.
2. the preparation method of the Sustained-release composite microbial fertilizer for Radix Codonopsis plantation according to claim 1, is characterized in that: in the 1st described step, the bacterial classification content in each bacterium liquid is 10 ~ 1,500,000,000/g.
3. the preparation method of the Sustained-release composite microbial fertilizer for Radix Codonopsis plantation according to claim 1, is characterized in that: the bake out temperature in the 3rd described step is 60 ~ 70 DEG C.
4. the preparation method of the Sustained-release composite microbial fertilizer for Radix Codonopsis plantation according to claim 1, is characterized in that: in the 4th described step, warm temperature is 50 ~ 60 DEG C, and churning time is 1 ~ 3 hour.
5. the preparation method of the Sustained-release composite microbial fertilizer for Radix Codonopsis plantation according to claim 1, is characterized in that: in the 4th described step, the molecular weight ranges of polyvinyl alcohol is 2000 ~ 4000.
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CN105481562A (en) * | 2015-12-23 | 2016-04-13 | 成都新柯力化工科技有限公司 | Special ecological fertilizer for improving content of scutellarein glucoside of codonopsis pilosula |
CN107568003A (en) * | 2017-09-21 | 2018-01-12 | 安徽亚泰天然植物科技有限公司 | A kind of implantation methods of Radix Codonopsis |
CN108821888A (en) * | 2018-08-28 | 2018-11-16 | 海南莲华生态文化股份有限公司 | A kind of compound fertilizer formula of water lily |
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