CN103288559A - Biological composite bacterial fertilizer special for salvia miltiorrhiza - Google Patents
Biological composite bacterial fertilizer special for salvia miltiorrhiza Download PDFInfo
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- CN103288559A CN103288559A CN2012104431341A CN201210443134A CN103288559A CN 103288559 A CN103288559 A CN 103288559A CN 2012104431341 A CN2012104431341 A CN 2012104431341A CN 201210443134 A CN201210443134 A CN 201210443134A CN 103288559 A CN103288559 A CN 103288559A
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- fertilizer
- red sage
- sage root
- microbial inoculum
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Abstract
The invention relates to biological composite bacterial fertilizer and belongs to the field of biological fertilizer. Biological composite bacterial fertilizer special for salvia miltiorrhiza comprises the following components in percentage by weight: 5 to 10 percent of a bacterial agent, 60 to 64 percent of a macromolecular natural organic matter, 3 to 5 percent of large granular urea, 9 to 11 percent of slow release coated urea and 12 to 20 percent of nitrogen phosphorus potassium composite fertilizer. The biological composite bacterial fertilizer special for the salvia miltiorrhiza has the advantages that the number and the activity of beneficial microorganisms are increased; harmful microorganisms are inhibited; the physical and chemical properties of soil are improved; soil nutrients are increased and balanced; and pesticide chemical fertilizer residues and other harmful matters of the soil are degraded. After the fertilizer is applied, the salvia miltiorrhiza can be planted in non-replanting and replanting fields repeatedly, so the quality and the yield of the salvia miltiorrhiza are improved.
Description
Technical field
The present invention relates to the microbial fertilizer field, particularly relate to a kind of red sage root special bio compound bacterial fertilizer.
Background technology
The red sage root (
Salvia miltiorrhizaBunge.) being the dry root of red sage root section per nnial herb, is one of maximum 40 kinds of traditional Chinese medicine materials of China's sales volume, has promoting blood circulation to remove blood stasis, swelling and pain relieving, the effect of nourishing blood to tranquillize the mind.Modern study shows, the red sage root can coronary artery dilator, increase coronary flow, improve core ischemia, infarct and heart function, adjusts the rhythm of the heart; Suppress thrombotic effect; Effects such as energy reducing blood-fat.
Along with going deep into of red sage root pharmacological research, red sage root range of application is more and more wider, and demand has cumulative year after year trend, depends merely on wild red sage root resource and can not satisfy the demands.Market supply in recent years is very in short supply, and national red sage root main producing region begins to carry out large-area red sage root artificial culture, to meet the need of market.But red sage root major production areas all is faced with a problem: the root rot that red sage root continuous cropping causes, blight, root knot nematode disease are having a strong impact on output and the quality of the main producing region red sage root.Medicinal herb grower's branch of main producing region is clipped to exotic species and plants medicinal material, causes red sage root main producing region output sharply to descend.Simultaneously, the red sage root of non-main producing region output, poor quality, curative effect is low.
The medicinal herb grower plants the red sage root mainly to execute inorganic fertilizer, and fertilising causes soil compaction, severe environmental pollution, utilization rate of fertilizer to reduce, and disease and pest increases, and causes planting cost increase, red sage root output and quality to descend, and benefit glides.At present, the red sage root is mainly based on base manure, and before full-bloom stage, imposing potash fertilizer and nitrogenous fertilizer is the fertilizing method of assisting simultaneously.The main drawback of this method is: the nitrogenous fertilizer in the base manure, and easily cause red sage root overground part growth in seedling stage vigorous, root growth is slow, causes later stage fertility deficiency, and output reduces.Topdress and be beneficial to growing of red sage root later stage root.But, spreading fertilizer over the fields, utilization rate of fertilizer is lower; Row replacement is taken a lot of work.Therefore, the fertilizer practice of the red sage root also needs to improve and upgrade.
Summary of the invention
The objective of the invention is to, overcome the deficiencies in the prior art, a kind of red sage root specific complex bacterial manure is provided, can effectively improve red sage root probiotics quantity, reduce red sage root soil-borne disease; Can single-dose application satisfy the required nutrition of each growth period of the red sage root; According to grow regulation of fertilizer requirement fertilising of the red sage root, a bulk application does not need to topdress, and avoids because of the soil pollution that overnutrition causes, the structure of improving the soil; Finally, reduce and produce red sage root cost, improve plantation red sage root benefit.
For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of red sage root special bio compound bacterial fertilizer, its composition and weight percent content are: microbial inoculum accounts for 5 ~ 10%, the macromole natural organic matter accounts for 45 ~ 50%, granular urea 12 ~ 14%, release-controlled coated urea 25 ~ 32%, phosphorus potassium complex fertilizer 5 ~ 15%, described microbial inoculum is made up of bacterial classification and solid medium, microbial inoculum and solid medium weight ratio 1:20, wherein the bacterial classification in the microbial inoculum be yellow green trichoderma (
Trichoderma aureoviride) W2256.
The yellow green trichoderma of described bacterial classification (
Trichoderma aureoviride) W2256 separation and Culture and mutagenesis gained from Trichoderma, this bacterial strain is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences; Preserving number: CGMCC No6494.Preservation date: on September 3rd, 2012.
Described macromole natural organic matter refers to that wherein a kind of of dregs of beans and Pericarppium arachidis hypogaeae, corn cob or stalk mixes according to weight 1:1.
Described phosphorus potassium complex fertilizer is made up of diammonium phosphate and vitriolate of tartar, wherein P
2O
5And K
2The weight ratio of O is 8:10.
Described solid medium is made up of wheat bran, acid-sludge and water, and its weight ratio is 2:1:1.33.
0.2 hundred million/gram of viable count content in the described microbial inoculum.
Red sage root special bio compound bacterial fertilizer preferred weight percent content proportioning of the present invention: microbial inoculum accounts for 8%, the macromole natural organic matter accounts for 47%, granular urea 13%, release-controlled coated urea 26%, phosphorus potassium complex fertilizer 6%.
Yellow green trichoderma (
Trichoderma aureoviride) W2256 separates mutagenic processes:
Separation method: the starting strain of this bacterium picks up from Jinan forest land of mountain area soil, uses PDA substratum+thousandth Streptomycin sulphate to carry out strain separating and cultivates, and carries out monospore then and separates, and obtains purebred yellow green trichoderma.After the continuation enlarged culturing obtains a large amount of conidiums, make spore spore content about 10 alive
7Individual/ml suspension, put and treat mutagenesis in the test tube.
Mutafacient system: use this institute
60Co-r x ray irradiation x device carries out mutagenesis to above bacteria suspension, and dosage range is selected 5-10kGry for use.According to it indexes such as cucumber fusarium axysporum bacteriostasis rate size, sporulation quantity are screened for bacterial strain after the mutagenesis, finally obtain yellow green trichoderma W2256.
The preparation of microbial inoculum
1) culture of strains
4 ℃ of mould W2256 bacterial classifications of wood are transferred to potato dextrose agar flat board (PDA) central authorities, 26 ℃ of cultivations.The wooden mould spore that growth on the PDA flat board is good inserts with the sterilized water dilution and is equipped with in the 250ml triangular flask of 100ml potato glucose (PA) nutrient solution, and 26 ℃, 180r/min, shaking culture 3 days is as bacterial classification.
2) enlarged culturing
Seed liquor 1.0ml is added in the liquid nutrient medium (Semen Maydis powder 15g/L, bean powder 5g/L add suitable quantity of water and boiled back 30 minutes, get juice with four layers of filtered through gauze, are diluted with water to 1000ml) that Semen Maydis powder and bean powder make 26 ℃ of shaking culture.
3) the fixing cultivation
Cultured trichoderma strain W2256 liquid moved into 5% ratio fill 100g wheat bran: in the 500ml triangular flask of the solid medium of acid-sludge: water=2:1:1.33,26 ℃ of cultivations.
4) the bacterium inspection is dried, and pulverizes
The present invention can effectively control soil-borne diseases such as red sage root root rot, blight, root knot nematode disease by adding yellow green trichoderma W2256 bacterial classification, and overcoming the red sage root can not continuous cropping and the problem of continuous cropping; According to the salviae nutrient demand, macroelement is carried out rational proportion, satisfy the red sage root desired nutritional that grows; Use the slowly-releasing fertilizer practice, supply with fertilizer according to red sage root regulation of fertilizer requirement, reduce fertilizer loss, improve red sage root quality and output; By adding organic macromolecule materials such as wheat bran and acid-sludge, improve Soil structure, for growing, the red sage root provides superior edatope.
Yellow green trichoderma W2256 microbial inoculum is effectively controlled red sage root root rot, blight, root knot nematode disease, and the phytopathogen of yellow green trichoderma W2256 has the antagonistic action of wide spectrum, and test shows that root rot, blight, root knot nematode disease pathogenic bacteria effect to the red sage root are better.Simultaneously, can also produce the plant and animal residues in the multiple enzyme decomposition soil, produce the organism that plant can be absorbed.
Granular urea, slowly-releasing bag Thio Urea: granular urea can satisfy the amount of the required nitrogenous fertilizer of red sage root early growth, avoids growth early stage nitrogenous fertilizer excessive, and the cauline leaf excessive growth that causes influences the quality and yield of the red sage root; Also nitrogenous fertilizer for want of not makes plant strain growth slow, reduces plant height, reduces output.Slowly-releasing bag Thio Urea mainly satisfies red sage root growth required nitrogenous fertilizer of middle and later periods.
Phosphorus potassium complex fertilizer: according to red sage root regulation of fertilizer requirement, adopt (0-8-10) phosphorus potassium complex fertilizer, wherein phosphate fertilizer adopts phosphorus potassium complex fertilizer with diammonium phosphate, potash fertilizer vitriolate of tartar, replenish the under-nutrition that single use urea causes, suitable proportioning can improve red sage root yield and quality.
The preparation of larger molecular organics: dregs of beans is pulverized, and mixes by weight 1:1 with a kind of of larger molecular organics Pericarppium arachidis hypogaeae, corn cob or stalk of other pulverizing.
Granular urea, release-controlled coated urea etc. are sold by market.
The invention has the beneficial effects as follows: red sage root biological composite bacterial manure has overcome because of root rot, blight, root knot nematode disease that continuous cropping causes by the biological control technology, improves land utilization ratio and yield rate; Red sage root biological composite bacterial manure is guaranteed its efficient comprehensive absorption in assurance fertilizer abundance, and the enzyme decomposition plant and animal residues that slowly-releasing bag Thio Urea control degradation of urea and yellow green trichoderma W2256 utilization produce becomes absorbable nutritive substance and carries out simultaneously.Slow released urea improves the soil microenvironment, is beneficial to the mould growth of wood; And the decomposition of larger molecular organics is accelerated in the mould adding of wood, and the microenvironment after decomposing more is conducive to the mould growth of wood, and soil obtains improvement, be beneficial to the absorption of its nutrition after the urea decomposition, complement each other between the two, do not have antagonistic action, make its industrialization become possibility.Wheat bran and acid-sludge provide nutrient for wood is mould, and are beneficial to the growth of bacterial classification.
Red sage root biological composite bacterial manure is an organic whole, and each effect is mutually promoted, and improves the microenvironment of soil from many aspects; and red sage root biological composite bacterial manure is according to red sage root regulation of fertilizer requirement; the rational application of fertilizers that provides can not cause secondary pollution, is conducive to environment protection.The Trichoderma group who selects for use can also provide the function of various active thing and various organized enzymes except having the phytopathogen antagonistic action comprehensively.
Embodiment
Embodiment 1
The configuration proportion of red sage root biological composite bacterial manure by weight percentage: microbial inoculum accounts for 8%, the macromole natural organic matter accounts for 47%, granular urea 13%, release-controlled coated urea 25%, phosphorus potassium complex fertilizer 6%.Mix pack.The macromole natural organic matter is that dregs of beans mixes according to weight 1:1 with corn cob.
Table 1 red sage root biological composite bacterial manure is to the influence of disease and pest and quality and yield
Table 1 data declaration is compared red sage root biological composite bacterial manure and can significantly be reduced root knot nematode disease, root rot sickness rate with common composite fertilizer; Improve TANSHINONES II a and content of danshinolic acid B; And improve its output.
Embodiment 2
The configuration proportion of red sage root biological composite bacterial manure by weight percentage: microbial inoculum accounts for 5%, macromole natural organic matter 45%, granular urea 12%, slowly-releasing bag Thio Urea 25%, phosphorus potassium complex fertilizer 13%.Mix pack.The macromole natural organic matter is that dregs of beans mixes according to weight 1:1 with Pericarppium arachidis hypogaeae.
Table 2 red sage root biological composite bacterial manure is to the influence of disease and output
The support of table 2 data, present embodiment red sage root biological composite bacterial manure can significantly suppress the root knot nematode disease and the root rot that cause because of continuous cropping; Compare output with common composite fertilizer and improve 50.6%.
Embodiment 3
The configuration proportion of red sage root biological composite bacterial manure by weight percentage: microbial inoculum accounts for 10%, the natural macromolecular organism accounts for 62%, granular urea 4%, slowly-releasing bag Thio Urea 10%, phosphorus potassium complex fertilizer 14%.Mix pack.The macromole natural organic matter is that dregs of beans mixes according to weight 1:1 with stalk.
Table 3 red sage root biological composite bacterial manure is to the influence of disease and quality and yield
Table 3 data declaration, present embodiment red sage root biological composite bacterial manure can not only reduce root knot nematode disease and root rot incidence, improve output; Simultaneously, improve tanshinone IIA and content of danshinolic acid B.
Embodiment 4
The configuration proportion of red sage root biological composite bacterial manure by weight percentage: microbial inoculum accounts for 5%, the natural macromolecular organism accounts for 48%, granular urea 12%, slowly-releasing bag Thio Urea 27%, phosphorus potassium complex fertilizer 8%.Mix pack.The macromole natural organic matter refers to that dregs of beans mixes according to weight 1:1 with Pericarppium arachidis hypogaeae.
Table 4 red sage root biological composite bacterial manure is to the influence of disease and output and surviving rate
Table 4 data declaration is compared present embodiment red sage root biological composite bacterial manure and has been reduced root knot nematode disease and the root rot sickness rate that causes because of continuous cropping with common composite fertilizer; Improve red sage root output; Strengthen red sage root keeping crop seedling from drought ability.(damage caused by a drought is more serious then, and plantation back 2 wheat harvesting periods just have effective precipitation.Keeping crop seedling from drought is windfall.
Claims (7)
1. red sage root special bio compound bacterial fertilizer, it is characterized in that: its composition and weight percent content are: microbial inoculum accounts for 5 ~ 10%, the macromole natural organic matter accounts for 45 ~ 50%, granular urea 12 ~ 14%, release-controlled coated urea 25 ~ 32%, phosphorus potassium complex fertilizer 5 ~ 15%, described microbial inoculum is made up of bacterial classification and solid medium, microbial inoculum and solid medium weight ratio 1:20, wherein the bacterial classification in the microbial inoculum be yellow green trichoderma (
Trichoderma aureoviride) W2256.
2. a kind of red sage root special bio compound bacterial fertilizer according to claim 1 is characterized in that: the yellow green trichoderma of described bacterial classification (
Trichoderma aureoviride) W2256 separation and Culture and mutagenesis gained from Trichoderma, this bacterial strain is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, and preserving number is CGMCC No.
6494
3. a kind of red sage root special bio compound bacterial fertilizer according to claim 1 is characterized in that: described macromole natural organic matter refers to that wherein a kind of of dregs of beans and Pericarppium arachidis hypogaeae, corn cob or stalk mixes according to weight 1:1.
4. according to the described a kind of red sage root special bio compound bacterial fertilizer of claim 1, it is characterized in that: described phosphorus potassium complex fertilizer is made up of diammonium phosphate and vitriolate of tartar, wherein P
2O
5And K
2The weight ratio of O is 8:10.
5. according to the described a kind of red sage root special bio compound bacterial fertilizer of claim 1, it is characterized in that: described solid medium is made up of wheat bran, acid-sludge and water, and its weight ratio is 2:1:1.33.
6. according to the described a kind of red sage root special bio compound bacterial fertilizer of claim 1, it is characterized in that: 0.2 hundred million/gram of viable count content in the described microbial inoculum.
7. according to claim 1,2,3,4 or 5 described a kind of red sage root special bio compound bacterial fertilizers, it is characterized in that: red sage root special bio compound bacterial fertilizer preferred weight percent content proportioning: microbial inoculum accounts for 8%, the macromole natural organic matter accounts for 47%, granular urea 13%, release-controlled coated urea 26%, phosphorus potassium complex fertilizer 6%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104016806A (en) * | 2014-06-24 | 2014-09-03 | 山东省农业科学院农产品研究所 | Special stubble-resistant biological bacterial fertilizer for radix salviae miltiorrhizae |
| CN104326836A (en) * | 2014-11-21 | 2015-02-04 | 中国农业科学院农业资源与农业区划研究所 | Stalk granular inorganic microorganism fertilizer |
| CN104892151A (en) * | 2015-05-27 | 2015-09-09 | 李孟平 | Preparation method of sustained-release composite microbial fertilizer for codonopsis pilosula planting |
| CN106007862A (en) * | 2016-06-25 | 2016-10-12 | 杨文平 | Special organic fertilizer for danshen root planting and preparation method of special organic fertilizer |
| CN113615539A (en) * | 2020-05-07 | 2021-11-09 | 蔡巨才 | Salvia miltiorrhiza cultivation medium and cultivation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113120A (en) * | 2007-07-09 | 2008-01-30 | 北京双色港农业科技开发有限公司 | Nano biological fertilizer |
| CN101638328A (en) * | 2008-10-14 | 2010-02-03 | 中国农业科学院农业资源与农业区划研究所 | Straw organic fertilizer and preparation method thereof |
| CN102503703A (en) * | 2011-11-22 | 2012-06-20 | 刘长福 | Novel compound fertilizer containing active microorganisms |
-
2012
- 2012-11-08 CN CN201210443134.1A patent/CN103288559B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101113120A (en) * | 2007-07-09 | 2008-01-30 | 北京双色港农业科技开发有限公司 | Nano biological fertilizer |
| CN101638328A (en) * | 2008-10-14 | 2010-02-03 | 中国农业科学院农业资源与农业区划研究所 | Straw organic fertilizer and preparation method thereof |
| CN102503703A (en) * | 2011-11-22 | 2012-06-20 | 刘长福 | Novel compound fertilizer containing active microorganisms |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104016806A (en) * | 2014-06-24 | 2014-09-03 | 山东省农业科学院农产品研究所 | Special stubble-resistant biological bacterial fertilizer for radix salviae miltiorrhizae |
| CN104326836A (en) * | 2014-11-21 | 2015-02-04 | 中国农业科学院农业资源与农业区划研究所 | Stalk granular inorganic microorganism fertilizer |
| CN104892151A (en) * | 2015-05-27 | 2015-09-09 | 李孟平 | Preparation method of sustained-release composite microbial fertilizer for codonopsis pilosula planting |
| CN106007862A (en) * | 2016-06-25 | 2016-10-12 | 杨文平 | Special organic fertilizer for danshen root planting and preparation method of special organic fertilizer |
| CN113615539A (en) * | 2020-05-07 | 2021-11-09 | 蔡巨才 | Salvia miltiorrhiza cultivation medium and cultivation method |
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