A kind of Ru 2006101161 and their purposes
[technical field]
The present invention relates to technical field of fertilizers.More specifically, the present invention relates to use the fertilizer of gamma-polyglutamic acid-preparation, also relate to the purposes of described fertilizer.
[background technology]
(gamma-polyglutamic acid-, γ-PGA) are the water-soluble polyamino acids that microbial fermentation produces to polyglutamic acid, are the high molecular polymers that is formed peptide bond by glutamic acid units by alpha-amino group and γ-carboxyl.Molecular weight distribution is 100kDa-10000kDa.Gamma-polyglutamic acid-has good water-absorbent, superpower adsorptivity and biodegradability; degraded product is non-harmful L-glutamic acid; it is a kind of good environment-friendly type macromolecule material; can be used as water-holding agent, adsorbent for heavy metal, flocculation agent, sustained release dosage and pharmaceutical carrier etc., very big commercial value and social value are all arranged in industries such as makeup, environment protection, food, medicine, agricultural, desertification control.Gamma-polyglutamic acid-salt, the gamma-polyglutamic acid-salt water-sol have similar character of gamma-polyglutamic acid-and same value.
At present, research both at home and abroad mainly concentrates on improvement and the gene studies that produces bacterium, fermenting process research and the research of extraction purge process, with and the production of derivative and the research of character, its applied research is few, and mainly concentrates on the wetting Agent for Printing Inks of pharmaceutical carrier, makeup, the applied research of dietary fiber food aspect.Agriculturally study lessly, mainly studied their disease resistance of plant, water-loss reducer of soil, the research of others, comprising the arable farming applied research just still less.
Chinese patent application 200610138655.0 relates to gamma-polyglutamic acid-(γ-PGA, the H form), gamma-polyglutamic acid-salt, gamma-polyglutamic acid-gel brine or contain gamma-polyglutamic acid-(γ-PGA, the H form), fermented liquid or its mixture of gamma-polyglutamic acid-salt (gamma-polyglutamic acid-salt), gamma-polyglutamic acid-gel brine, promoting plant growth or seed growth, increase crop yield, improving the application that suppresses aspect the pathogenic bacteria.This patent disclosure the preparation method of gamma-polyglutamic acid-salt and hydrogel thereof, the gamma-polyglutamic acid-of different concns and salt thereof, different concns gamma-polyglutamic acid-gel brine are also disclosed as the influence to soil function of moistening agent, complexing agent and sequestrant; Disclose the application of seed pelleting, using method is for directly using.But this patent coverage concentration range too wide, that provide is too big, and concentration is only arranged, and lacks usage quantity, to producing the actual directive significance that do not have.If use by its high density, cost is relatively more expensive, is not suitable for agriculture production, and this patent only relates to seed pelleting and directly use on using method, uses inconvenience, has increased labor capacity and production cost.
[summary of the invention]
[technical problem that will solve]
The purpose of this invention is to provide a kind of Ru 2006101161.
Another object of the present invention provides the purposes of described Ru 2006101161.
[technical scheme]
People know that gamma-polyglutamic acid-contains terminal amine and a plurality of COO
--Group, the NH of nitrogenous fertilizer
4 +, the K in the potash fertilizer
+, the Ca of secondary nutrients
2+, Mg
2+, trace element fertilizer Zn
2+Deng can be directly and the carboxyl site of gamma-polyglutamic acid-and amide group site bonding, form water miscible, stable gamma-polyglutamic acid-salt, provide nutrition for plant growth lentamente.
Gamma-polyglutamic acid-can have multiple conformation: alpha-helix, and random coil, beta sheet, helix-coil transition district, parcel are coalescent etc.The pH of fertilizer is mostly at 5-8, and the main existence form of gamma-polyglutamic acid-is the binding form.During with some metal ion-chelant, it is coalescent that conformation changes parcel into from random coil.So also can delay fertilizer and discharge nutritive element.
Therefore, the applicant utilizes these characteristics of gamma-polyglutamic acid-to make the present invention.
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of Ru 2006101161, it is characterized in that the composed as follows of it, in weight part:
Gamma-polyglutamic acid-20-500 weight part;
Fertilizer 10000-60000 weight part;
Sorbent material 0-75000 weight part;
Described gamma-polyglutamic acid-is gamma-polyglutamic acid-, gamma-polyglutamic acid-salt or gamma-polyglutamic acid-gel brine or the fermented liquid that contains gamma-polyglutamic acid-, gamma-polyglutamic acid-salt or gamma-polyglutamic acid-gel brine; The molecular weight of described polyglutamic acid is 800-8000kDa.
Described gamma-polyglutamic acid-is the water-soluble polyamino acid that produces by microbial fermentation, and it is to form the high molecular polymer that peptide bond constitutes by glutamic acid units by alpha-amino group and γ-carboxyl, and its molecular weight distribution is that 100kDa is to 10000kDa.
As the raw material of preparation Ru 2006101161 of the present invention, gamma-polyglutamic acid-should have following characteristics: outward appearance is little yellow or yellow powder; Its active constituent content 〉=30%; Molecular weight is at 800kDa-8000kDa; The pH value is at 4-8.5; Can not contain heavy metal.The fermented liquid that use contains gamma-polyglutamic acid-, gamma-polyglutamic acid-salt or gamma-polyglutamic acid-gel brine is during as raw material, and its concentration is not crucial, but will control its gamma-polyglutamic acid-significant quantity, guarantees in the scope of above-mentioned composition.
In the present invention, the gamma-polyglutamic acid-of use for example is the product produced by applicant oneself or by Taiwan powder or the fermented liquid that red company provides of distinguishing the flavor of.After need dissolving, just can use in the gamma-polyglutamic acid-powder.Perhaps, can or prepare the gamma-polyglutamic acid-of the present invention's use by the method for " the scientific and technological communication of fermenting " 4 phases in 2006 record such as " gamma-polyglutamic acid-production technology and the application " document of etc.ing according to Chinese patent ZL01127287.2ZL200610155277.7 etc.
The method of described gamma-polyglutamic acid-produced according to the present invention will be described below.
At first prepare fermented liquid:
Adopt the disclosed subtilis of the applicant's patent application (application number 200710130248.X) (Bacillus subtillis) CGMCC No.2108, slant culture through routine, shake-flask culture, being inoculated in following fermention medium cultivates: glucose 40-60g/l, yeast extract paste 5-9g/l, Sodium Glutamate 40-60g/l, dipotassium hydrogen phosphate 2-6g/l, sal epsom 0.25g/l, pH=7, through 37 ℃, 150 rev/mins of rotating speeds, ventilation obtains fermented liquid of the present invention than cultivating 96 hours under the 1:1 condition.
Then prepare gamma-polyglutamic acid-:
Above-mentioned fermented liquid is got the 20ml fermented liquid be put in the 500ml triangular flask, add and in-18 ℃ of refrigerators, place the ice dehydrated alcohol 40ml that spends the night, concussion, until there being precipitation to produce, to precipitate taking-up, put into 50 ℃ of baking oven bakings 12 hours, and pulverize and obtain described gamma-polyglutamic acid-.
On meaning of the present invention, described sorbent material should be appreciated that it is a kind of porous mass that can adsorb gamma-polyglutamic acid-or fertilizer solution.The fineness of this sorbent material is more than or equal to 80 orders, and absorption ratio (fermented liquid: sorbent material) greater than 1/2.
Described sorbent material is selected from corn cob, straw powder, natural zeolite, turfy soil, volcanic ash, flyash or wilkinite.
Preferably, described sorbent material is selected from corn cob, straw powder, turfy soil, flyash or wilkinite.
More preferably, described sorbent material is selected from corn cob, straw powder or turfy soil.
In the present invention, described gamma-polyglutamic acid-salt generally is gamma-polyglutamic acid-sodium, potassium, magnesium, calcium or ammonium salt.
According to the present invention, any mixture of these gamma-polyglutamic acid-sodium, potassium, magnesium, calcium or ammonium salt and single gamma-polyglutamic acid-salt have same implementation result, so they are also all within protection scope of the present invention.
Preferably, described gamma-polyglutamic acid-salt is gamma-polyglutamic acid-sodium, potassium, magnesium or ammonium salt.
More preferably, described gamma-polyglutamic acid-salt is gamma-polyglutamic acid-potassium, sodium or ammonium salt.
In the present invention, described fertilizer is one or more fertilizer that are selected from nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, secondary nutrients, trace element fertilizer, nitrogen-phosphorus-potassium compound fertilizer, biological organic fertilizer, compound organic and inorganic fertilizer, amino acid fertilizer or humic acid fertilizer.
Described nitrogenous fertilizer for example is selected from urea, ammonium nitrate, ammonium sulfate, saltpetre or ammonium phosphate.
Described potash fertilizer for example is selected from Repone K, vitriolate of tartar, saltpetre or potassium primary phosphate.
Described phosphate fertilizer for example is selected from potassium primary phosphate, ammonium phosphate, secondary calcium phosphate, calcium metaphosphate, thomas phosphate or active phosphorus breeze.
Described secondary nutrients for example is selected from calcareous fertilisers, as nitrocalcite, lime carbonate, normal superphosphate; Fertiliser containing magnesium is as sal epsom, magnesium chloride, fused(calcium magnesium)phosphate; Sulfur fertilizer is as sal epsom, ammonium sulfate, vitriolate of tartar etc.
Described trace element fertilizer is selected from boron, iron, manganese, zinc, copper or molybdenum trace element fertilizer.Boron fertilizer is for example as boric acid, borax, boric magnesium fertilizer; Iron fertilizer is ferrous sulfate, ferrous ammonium sulphate for example; Manganese fertilizer is as manganous sulfate, Manganous chloride tetrahydrate; Zinc fertilizer is for example as zinc sulfate, zinc oxide, zinc chloride; Copper for example is copper sulfate; Molydbenum fertilizer for example is ammonium molybdate, Sodium orthomolybdate etc.
The nitrogen-phosphorus-potassium compound fertilizer of Shi Yonging, biological organic fertilizer, compound organic and inorganic fertilizer, amino acid fertilizer or humic acid fertilizer all are product solds in the market in the present invention.In the present invention the nitrogen-phosphorus-potassium compound fertilizer of Shi Yonging for example be in the Qinhuangdao Ah GSFC with trade(brand)name " Sa Kefu " product sold; Described biological organic fertilizer for example is Weifang and believes that bio tech ltd is with " standing grain letter " brand product sold; Described compound organic and inorganic fertilizer for example is that lotus flavor precious fertile company limited already in Henan is with " the lotus flavor is precious " brand product sold; Described amino acid fertilizer for example is that Beijing Inst. of Gardens Science is with trade(brand)name " Ke Libao " product sold; Described humic acid fertilizer for example is that grand biotechnology company limited is contained with " Jin Wei " brand product sold in Shandong.
In the present invention, the ratio of each fertilizer material component need be adjusted according to particular cases such as soil, weather condition, crop varieties and crop growth periods, prepares according to the soil testing and fertilizer recommendation principle.
Can adopt method known to those skilled in the art, described Ru 2006101161 of the present invention is made granule, pulvis, solution, suspension or stylus.These formulations need those skilled in the art to determine according to various factorss such as soil, weather condition, crop varieties.
Fertiliser granulates agent preparation method for example of the present invention: can take by weighing gamma-polyglutamic acid-and fertilizer respectively according to the ratio of each component in the above-mentioned Ru 2006101161, for example taking by weighing 40-50 weight part gamma-polyglutamic acid-or gamma-polyglutamic acid-salt is added in temperature 40-80 ℃ the 1000-5000 weight parts water, stirring and dissolving obtained gamma-polyglutamic acid-solution in 1 hour, used 0.1-0.2MP then
aPressurized air this solution is injected in the rotary drum on the 10000-30000 weight part granular urea, the rotating speed of this rotary drum is 60-150 rev/min, blasts 25-100 ℃ of air simultaneously, obtains granulated fertilizer of the present invention after the drying.
Fertilizer pulvis preparation method of the present invention for example is, can take by weighing gamma-polyglutamic acid-and fertilizer respectively according to the ratio of each component in the above-mentioned Ru 2006101161, for example take by weighing 40-50 weight part gamma-polyglutamic acid-or gamma-polyglutamic acid-salt, be added in 40-80 ℃ the 1000-5000 weight parts water, stirring and dissolving 1 hour, use sorbent material to adsorb then, mix with Powdered fertilizer again, obtain fertilizer pulvis of the present invention after the drying.Certainly, this pulvis mixes with normally used in the art suitable amount of adhesive, and through disk or drum granulating or extruding pelletization, drying obtains granule then.
Certainly, adopt usual method known to those skilled in the art to prepare solution, suspension or stylus.
People know, gamma-polyglutamic acid-is compared with most of protein, can form four types hydrogen combination in per three the successive L-glutamic acid of gamma-polyglutamic acid-, and a hydrogen combination is only arranged in per 3.6 amino-acid residues in other protein, so gamma-polyglutamic acid-has especially strong water-absorbent.The bacterial classification that microbial method is produced gamma-polyglutamic acid-is generally subtilis or Bacillus licheniformis, it itself is exactly agricultural anti-disease microbial, so directly use the fermented liquid that contains gamma-polyglutamic acid-, its active bacterial strain can improve the disease resistance of plant, thereby reduce the generation of Plant diseases, play the effect of volume increase.In view of above mechanism, use and contain gamma-polyglutamic acid-, gamma-polyglutamic acid-salt, gamma-polyglutamic acid-gel brine or contain the fermented liquid of gamma-polyglutamic acid-, gamma-polyglutamic acid-salt, gamma-polyglutamic acid-gel brine or the chemical fertilizer of its mixture, can play water conservation, protect fertilizer, slowly-releasing, disease-resistant effect, thereby reach the purpose that increases production and improves productivity.
The invention still further relates to the purposes of described Ru 2006101161, be used for lawn, farm crop, vegetables, flowers, forest cultivation, play the effect of protecting fertilizer, water conservation, defending and fighting against diseases, fertilizer slow release, raising utilization rate of fertilizer.
When Ru 2006101161 of the present invention was applied to the lawn, general amount of application was 10-100 kilogram/mu; When Ru 2006101161 of the present invention was applied to food crop, general amount of application was 20-60 kilogram/mu; When Ru 2006101161 of the present invention was applied to vegetables, general amount of application was 20-80 kilogram/mu; When Ru 2006101161 of the present invention was applied to flowers, general amount of application was 20-50 kilogram/mu; When Ru 2006101161 of the present invention was applied to forest, general amount of application was 20-60 kilogram/mu; But all need to adjust according to particular cases such as soil, weather condition, kind and vegetative period.
Ru 2006101161 of the present invention can be with base manure, fertilization mode such as topdress, spray applies fertilizer.
[beneficial effect]
The various formulations of Ru 2006101161 of the present invention, can directly use as a kind of new slow release fertilizer, simply, convenient, reduce loss of chemical fertilizer, reduce fertilizer amount, improve chemical fertilizer utilization ratio, compare with the equivalent nutritive element chemical fertilizer of generally using, can reduce fertilizer amount 20-30%, volume increase 10-15%; Save production cost, reduce environmental pollution.
[embodiment]
In order to further specify the present invention, will realize purpose of the present invention and other characteristics and advantage by following embodiment and EXPERIMENTAL EXAMPLE, yet they can not limit protection scope of the present invention.
Embodiment 1
40 grams according to the homemade gamma-polyglutamic acid-of the inventor, are added in 80 ℃ 2667 ml waters, and stirring and dissolving 1 hour obtains the gamma-polyglutamic acid-aqueous solution.Take by weighing the granular compound fertilizer (N-P that Ah GSFC sells with trade(brand)name " Sa Kefu " in 25 kilograms of Qinhuangdao then
2O
5-K
2O 15-15-15), is added in the rotary drum granulator of the upright mountain machinofacture company limited in Siping City with " upright mountain " brand sale, when the rotary drum rotating speed of this rotary drum granulator reaches 80 rev/mins, uses 0.1MP
aPressurized air is ejected on the granular compound fertilizer in this rotary drum with the gamma-polyglutamic acid-aqueous solution of 200 ml/min flows with preparation, feeds 80 ℃ of hot blasts simultaneously, carries out drying, obtains granulated fertilizer of the present invention after the cooling.
Directly as the base manure maize planting, it is 40 gram gamma-polyglutamic acid-/mus that amount of application is amounted to pure gamma-polyglutamic acid-consumption with 25 kilograms of resulting granules fertilizer, and the later stage need not topdress again.Simultaneously, use the granular compound fertilizer (N-P that Ah GSFC sells with trade(brand)name " Sa Kefu " in the same Qinhuangdao
2O
5-K
2O15-15-15), according to same amount of application, same field management management.Use corn yield that fertilizer of the present invention reaches than not using the high by 12.8% of gamma-polyglutamic acid-.
Embodiment 2:
40 grams according to the homemade gamma-polyglutamic acid-of the inventor, are added in 60 ℃ 1333 ml waters, and stirring and dissolving 1 hour obtains the gamma-polyglutamic acid-aqueous solution.Take by weighing the urea that 15 kilograms of Shandong Luxi Chemical Industry Co., Ltd sell with " western Shandong " brand then; be added in the rotary drum granulator of the upright mountain machinofacture company limited in Siping City with " upright mountain " brand sale; when the rotary drum rotating speed of this rotary drum granulator reaches 70 rev/mins, use 0.1MP
aPressurized air is ejected into the gamma-polyglutamic acid-aqueous solution for preparing on the granular urea in this rotary drum with the flow of 100 ml/min, feeds normal temperature wind simultaneously, carries out drying, obtains granulated fertilizer of the present invention after the cooling.
Embodiment 3:
50 grams by the homemade gamma-polyglutamic acid-sodium of the inventor, are added in 40 ℃ 5000 ml waters, and stirring and dissolving 1 hour obtains the gamma-polyglutamic acid-aqueous solution.Take by weighing the biological organic fertilizer that 50 kilograms of our company sell with " Fu Side " brand then, be added in the rotary drum granulator of the upright mountain machinofacture company limited in Siping City with " upright mountain " brand sale, when the rotary drum rotating speed of this rotary drum granulator reaches 100 rev/mins, use 0.1MP
aPressurized air is ejected on the granular biological fertilizer in this rotary drum with the gamma-polyglutamic acid-aqueous solution of 100 ml/min flows with preparation, feeds 40 ℃ of hot blasts simultaneously, carries out drying, obtains granulated fertilizer of the present invention after the cooling.
Directly as base manure plantation rose, it is 50 gram gamma-polyglutamic acid-/mus that amount of application is amounted to pure gamma-polyglutamic acid-consumption with 50 kilograms of resulting granules fertilizer.Simultaneously, use 55 kilograms/mu of the biological organic fertilizers that same our company sells with " Fu Side " brand, same field management.The two greenhouse growing way etc. are as broad as long.Can prove thus, use the fertilizer that is added with gamma-polyglutamic acid-and can save fertilizer 9%.
Embodiment 4:
Take by weighing the granular compound fertilizer (N-P that Ah GSFC sells with trade(brand)name " Sa Kefu " in 25 kilograms of Qinhuangdao
2O
5-K
2O 15-15-15), is added in the rotary drum granulator of the upright mountain machinofacture company limited in Siping City with " upright mountain " brand sale, when the rotary drum rotating speed of this rotary drum granulator reaches 120 rev/mins, uses 0.2MP
aPressurized air is ejected on the granular compound fertilizer in this rotary drum by the homemade gamma-polyglutamic acid-fermented liquid of the inventor 1000 milliliters with the flow of 100 ml/min, feeds normal temperature wind simultaneously, carries out drying, obtains granulated fertilizer of the present invention after the cooling.
Embodiment 5:
40 grams by the homemade gamma-polyglutamic acid-of the inventor, are added in 80 ℃ 2000 ml waters, and stirring and dissolving 1 hour obtains the gamma-polyglutamic acid-aqueous solution.Be adsorbed in then on 2 kilogram of 80 purpose corn cob.Take by weighing the urea that 10 kilograms of Shandong Luxi Chemical Industry Co., Ltd sell with " western Shandong " brand, take by weighing the monoammonium phosphate that 6 kilograms of Kunming chemical fertilizer limited liability companys sell with " Shi Mei " board, take by weighing the vitriolate of tartar that 3 kilograms of Shijiazhuang and combination worker chemical fertilizer company limited sell with " with closing " board, add the corn cob after adsorbing, puddle even dry back and become pulvis of the present invention.
Above pulvis is added suitable amount of adhesive mix, the tablets press granulating and drying of selling with " Bai Shi " board with the white stone bio-feritlizer in Harbin company becomes granule of the present invention.
Directly as the base manure rice cultivation, it is 40 gram gamma-polyglutamic acid-/mus that amount of application is amounted to pure gamma-polyglutamic acid-consumption with 21 kilograms of resulting granules fertilizer.Simultaneously, use the not interpolation gamma-polyglutamic acid-fertilizer of preparation according to the above ratio of equivalent to make base manure, same topdresses, same field management management.Use rice yield that fertilizer of the present invention reaches than not using the high by 14.3% of gamma-polyglutamic acid-.
Embodiment 6:
With 1000 milliliters by the homemade gamma-polyglutamic acid-fermented liquid of the inventor, be adsorbed on 1 kilogram of 80 purpose corn cob.Take by weighing the urea that 10 kilograms of Shandong Luxi Chemical Industry Co., Ltd sell with " western Shandong " brand, take by weighing the monoammonium phosphate that 6 kilograms of Kunming chemical fertilizer limited liability companys sell with " Shi Mei " board, take by weighing the vitriolate of tartar that 3 kilograms of Shijiazhuang and combination worker chemical fertilizer company limited sell with " with closing " board, add the corn cob after adsorbing, puddle even dry back and become pulvis of the present invention.
Above pulvis is added suitable amount of adhesive mix, the tablets press granulating and drying of selling with " Bai Shi " board with the white stone bio-feritlizer in Harbin company becomes granule of the present invention.
Embodiment 7:
Take by weighing the urea that 8 kilograms of Shandong Luxi Chemical Industry Co., Ltd sell with " western Shandong " brand, take by weighing the monoammonium phosphate that 7 kilograms of Kunming chemical fertilizer limited liability companys sell with " Shi Mei " board, take by weighing the vitriolate of tartar that 6 kilograms of Shijiazhuang and combination worker chemical fertilizer company limited sell with " with closing " board, take by weighing the sal epsom that 3 kilograms of Lianyun Harbour patina system magnesium company limiteds sell with " Hai Ying " board, take by weighing the zinc sulfate that grand sharp chemical plant, 0.8 kilogram of Fuyang City produces, add in 80 ℃ 20 kg water, 100 rev/mins were stirred 15 minutes, 50 grams are added by the homemade gamma-polyglutamic acid-of the inventor, continue to stir 30 minutes.Use 0.1MP then
aPressurized air solution be pressed into Qidong of adorning 75 kilograms of the peats composed of rotten mosses converge in the vertical technology mixing machine that Long Hunheshebeichang makes, puddle and became pulvis of the present invention in 30 minutes.
Above pulvis is added suitable amount of adhesive mix, the throwing circle tablets press granulating and drying that adopts Harbin white stone bio-feritlizer company to sell with " Bai Shi " board becomes granule of the present invention.
Directly as base manure plantation rye grass, it is 50 gram gamma-polyglutamic acid-/mus that amount of application is amounted to pure gamma-polyglutamic acid-consumption with double centner resulting granules fertilizer.Simultaneously, use the not interpolation gamma-polyglutamic acid-fertilizer of preparation according to the above ratio of equivalent, same field management management.The lawn density of using fertilizer of the present invention to plant was all once waiting all to be better than does not add gamma-polyglutamic acid-fertilizer, and holds the green phase than not adding gamma-polyglutamic acid-fertilizer long 10 days.
Embodiment 8:
Take by weighing the urea that 8 kilograms of Shandong Luxi Chemical Industry Co., Ltd sell with " western Shandong " brand, take by weighing the monoammonium phosphate that 7 kilograms of Kunming chemical fertilizer limited liability companys sell with " Shi Mei " board, take by weighing the vitriolate of tartar that 6 kilograms of Shijiazhuang and combination worker chemical fertilizer company limited sell with " with closing " board, take by weighing the sal epsom that 3 kilograms of Lianyun Harbour patina system magnesium company limiteds sell with " Hai Ying " board, take by weighing the zinc sulfate that grand sharp chemical plant, 0.8 kilogram of Fuyang City produces, add in 50 ℃ 20 kg water, 100 rev/mins were stirred 15 minutes, 1000 milliliters are added by the homemade gamma-polyglutamic acid-fermented liquid of the inventor, continue to stir 30 minutes.Use 0.1MP then
aPressurized air solution be pressed into Qidong of adorning 75 kilograms of the peats composed of rotten mosses converge in the vertical technology mixing machine that Long Hunheshebeichang makes, puddle and became pulvis of the present invention in 30 minutes.
Above pulvis is added suitable amount of adhesive mix, the throwing circle tablets press granulating and drying that adopts Harbin white stone bio-feritlizer company to sell with " Bai Shi " board becomes granule of the present invention.
Embodiment 9:
With the gamma-polyglutamic acid-of homemade 60 grams of the inventor, directly add 20 kilograms of Shandong and contain in the liquid humic acid fertilizer of grand biotechnology company limited with the sale of " Jin Wei " board, stirred 1 hour, make solution of the present invention.
Above invention fertilizer is used for cucumber for 20 kilograms topdresses, seven days once; Use the Shandong of equivalent simultaneously and contain the liquid humic acid fertilizer of grand biotechnology company limited.Using fertilizer of the present invention can be than the fertilizer volume increase 13.2% of not adding gamma-polyglutamic acid-.
Embodiment 10:
The gamma-polyglutamic acid-fermented liquid that the inventor is homemade 1500 milliliters directly adds 20 kilograms of Shandong and contains in the liquid humic acid fertilizer of grand biotechnology company limited with the sale of " Jin Wei " board, stirs 1 hour, makes fertilizer solution of the present invention.
Experiment embodiment 1
The research gamma-polyglutamic acid-is to the influence of utilization rate of fertilizer.
Choose 4 * 10=40m
29 of sub-districts.Experimental design is that A does not apply fertilizer, and B uses the composite fertilizer (N-P that Ah GSFC sells with trade(brand)name " Sa Kefu " in 25 kilograms of/mu Qinhuangdao
2O
5-K
2O15-15-15), C uses 20 kilograms/mu of the composite fertilizers (its nitrogen, phosphorus, potassium content are identical with B) of the embodiment of the invention 1 that contains 40 gram gamma-polyglutamic acid-s.Each is handled three times and repeats.Planting plant is corn.Choose after the maturation and represent plant, it is pulverized, the content of the nitrogen of mensuration plant, phosphorus, potassium, its content is represented with weight %.
Calculate utilization rate of fertilizer according to following formula:
Table one: use the utilization rate of fertilizer contrast table that contains and do not contain gamma-polyglutamic acid-
Nutrient element |
Treatments B plant recovery of nutrient (%) |
Handle C plant recovery of nutrient (%) |
Increasing amount (%) |
Nitrogen |
27.6 |
46.3 |
18.7 |
Phosphorus |
16.2 |
27.3 |
11.1 |
Potassium |
37.8 |
53.8 |
16.0 |
Be clear that by table one utilization rate of fertilizer of handling C is than the obvious raising of treatments B.
EXPERIMENTAL EXAMPLE 2
Research contains the biological degradation rate of the fermented liquid of gamma-polyglutamic acid-salt, infers its slowly-releasing hold-time.
Get topsoil soil 500 grams apart from face of land 20cm, add in 1000 ml sterile waters, stirred postprecipitation 30 minutes, filter with coarse filter paper, discard preceding 200 milliliters of filtrates, residual filtrate is as inoculation liquid.1600ml 30 grams per liter gamma-polyglutamic acid-fermented liquids are divided into 16 parts.Inoculate 20 milliliters of described inoculation liquid for every part then, a copy of it is not inoculated inoculation liquid in contrast.Place under the room temperature then.Got a 120ml inoculation filtrate every 10 days, at ambient temperature the polyglutamic acid in the filtrate is precipitated out, adopt its throw out of filtering separation then, use distilled water wash again three times, then under 50 ℃ of conditions dry 12 hours with dehydrated alcohol.
Adopt this method to study gamma-polyglutamic acid-in the degraded in soil rate over time, it the results are shown in table two.
Calculate degradation rate according to following formula:
Quality * 100% of gamma-polyglutamic acid-in degradation rate=(quality of back gamma-polyglutamic acid-is placed in the quality of gamma-polyglutamic acid-in the contrast-inoculation)/contrast
Table two: the polyglutamic acid degradation rate over time
Time (my god) |
10 |
20 |
30 |
40 |
50 |
60 |
70 |
80 |
90 |
100 |
110 |
120 |
130 |
140 |
150 |
Degradation rate (%) |
12.1 |
25.0 |
35.1 |
42.0 |
50.5 |
589 |
65.7 |
71.9 |
78.1 |
83.7 |
87.8 |
90.1 |
91.7 |
93.8 |
95.0 |
Infer that thus the slow releasing function time of gamma-polyglutamic acid-in soil is more than five months.Prove also that simultaneously gamma-polyglutamic acid-can degrade fully in soil, and can not increase new pollution.
EXPERIMENTAL EXAMPLE 3
Slow release effect after research gamma-polyglutamic acid-salt and the chemical fertilizer combination.
With the granule double centner of the embodiment of the invention 7 preparations, as 667m
2Rye grass plantation base manure, not spraying fertilizer.Use nutrient identical simultaneously, but the fertilizer that does not have gamma-polyglutamic acid-salt in contrast with content.Respectively sowing 2 months with 4 months the time, adopt the fertilizer industry national standard to analyze N in the soil, P
2O
5, K
2The content of O, these the results are shown in table three.
Table three: use the fertilizer that contains gamma-polyglutamic acid-salt and the slow release effect of conventional fertilizer
By the data of this table as seen, the slow release effect of gamma-polyglutamic acid-salt is very obvious.
EXPERIMENTAL EXAMPLE 4
The effective level of research gamma-polyglutamic acid-.
Choose 4*10=40m
224 of sub-districts.Experimental design is:
A uses 20 kilograms of/mu (N-P of composite fertilizer
2O
5-K
2O 15-15-15).
B uses the composite fertilizer that 20 kilograms of/mu gamma-polyglutamic acid-total amounts are 10 grams, the same A of nutrient content.
C uses the composite fertilizer that 20 kilograms of/mu gamma-polyglutamic acid-total amounts are 20 grams, the same A of nutrient content.
D uses the composite fertilizer that 20 kilograms of/mu gamma-polyglutamic acid-total amounts are 30 grams, the same A of nutrient content.
E uses the composite fertilizer that 20 kilograms of/mu gamma-polyglutamic acid-total amounts are 40 grams, the same A of nutrient content.
F uses the composite fertilizer that 20 kilograms of/mu gamma-polyglutamic acid-total amounts are 60 grams, the same A of nutrient content.
G uses the composite fertilizer that 20 kilograms of/mu gamma-polyglutamic acid-total amounts are 80 grams, the same A of nutrient content.
H uses the composite fertilizer that 20 kilograms of/mu gamma-polyglutamic acid-total amounts are 100 grams, the same A of nutrient content.
Each is handled to be and makees base manure and use, and topdressing is to impose 5.6 kilograms/mu in urea in per 7 days behind the result, and 4 kilograms/mu in Repone K imposes 7 times continuously.Planting vegetable is a cucumber.Each meter of plucking produces.
Table four: various processing cucumber yield tables
Proved that by The above results when being applied to green cucumber, the gamma-polyglutamic acid-effective level is more than the 20 gram/mus, when consumption was 40-60 gram/mu, amount of increase in production was bigger, and when consumption 60 gram/mus were above, amount of increase in production changed little.Its optimum amount is a 40-60 gram/mu.
EXPERIMENTAL EXAMPLE 5
The volume increase fat-reducing effect of research gamma-polyglutamic acid-.
Choosing 4 of 1 mu of branch sub-districts tests.The experiment crop is a paddy rice.
Experimental design is as follows:
" Sa Kefu " composite fertilizer (N-P that Ah GSFC produces in 25 kilograms of/mu Qinhuangdao of A
2O
5-K
2O 15-15-15) makees base manure, impose 5 kilograms/mu in ear manuer urea, 2.5 kilograms/mu in Repone K; Field management is the conventional cultivation management.
25/ mu of kilogram composite fertilizer of granule (N-P that B uses embodiment 1 to make
2O
5-K
2O15-15-15), fertilizer amount, usage and cultivation management are with handling A.
The amount that C should handle is 80% of a treatments B consumption, and other are with handling A.
The amount that D should handle is 70% of a treatments B consumption, and other are with handling A.
The amount that E should handle is 60% of a treatments B consumption, and other are with handling A.
Table five: experiment is handled and output
Content |
Output (kilogram/mu) |
Percentage increases (%) |
Contrast A |
539.4 |
|
Treatments B |
616.5 |
14.3 |
Handle C |
576.1 |
6.8 |
Handle D |
556.4 |
3.2 |
Handle E |
510.8 |
-5.3 |
By this table result as can be seen, the action effect of gamma-polyglutamic acid-can reduce fertilizer amount 30% for volume increase 14.3%.