CN103477901B - Nutritive cup provisional-planting substrate and provisional-planting method for tissue culture seedlings of downy grapes - Google Patents
Nutritive cup provisional-planting substrate and provisional-planting method for tissue culture seedlings of downy grapes Download PDFInfo
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- CN103477901B CN103477901B CN201310322317.2A CN201310322317A CN103477901B CN 103477901 B CN103477901 B CN 103477901B CN 201310322317 A CN201310322317 A CN 201310322317A CN 103477901 B CN103477901 B CN 103477901B
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Abstract
The invention relates to a nutritive cup provisional-planting substrate and a nutritive cup provisional-planting method for tissue culture seedlings of downy grapes. The method comprises the following steps of (1) preparing a downy grape provisional-planting substrate from 45 to 50 volume percent of red mud, 40 to 50 volume percent of peat and 1 to 5 volume percent of decayed farm manure, and placing the downy grape provisional-planting substrate into a non-woven nutritive cup; (2) selecting seedlings, namely selecting tissue culture seedlings with complete root systems and complete leaves; (3) performing provisional-planting, namely transferring the tissue culture seedlings in the step (2) into the non-woven nutritive cup in the step (1) for provisional-planting. According to the provisional-planting method for the tissue culture seedlings of the downy grapes, the root systems of the seedlings in the growth process of the downy grapes are complete, and a great number of lateral roots are formed, so that the survival rate of the seedlings is increased, and the seedlings can well grow in middle and later periods.
Description
Technical field
The present invention relates to the seedling breeding technology of downy grape, particularly the plantlet in vitro nutrition cup heeling in technology of downy grape.
Background technology
Downy grape is one of vegetative kingdom floristics.This plant, on plant classification table, is attributed to Angiospermae (Angiospermae) Dicotyledoneae (Magnoliopsida) Rhamnales (Rhamnales) Vitaceae (Vitaceae).Downy grape fresh fruit is containing nutriments such as multivitamin, each seed amino acid, mineral matters, also containing abundant anthocyanidin disaccharide glycosides, superoxide dismutase (SOD) and resveratrol (RES), there is the effects such as softening blood vessel, anti-ageing, anti-cancer.The feature of downy grape is that acid is high, sugar is low, skin depth, tannin are many, pigment is dense, fragrance is unique, is the good raw material brewageing claret.Utilize downy grape berry for raw material, brewage the wine processed, with its pure natural, green, free of contamination feature, deeply by consumers.Along with improving constantly of wild Vitis quinquangularis wine popularity and understanding degree, the demand of market to downy grape is surged, and occurs the problem of raw material supply difficulty.The output of wild Vitis quinquangularis can not meet the demand in market, in the urgent need to carrying out artificial planting.Scientific and technical personnel, when resource investigation, have found the first strain initial species hermaphrodite flower wild Vitis quinquangularis individual plant in the world, and this is found to be wild Vitis quinquangularis and realizes artificial planting and provide condition.In March, 2011, ' wild No. 2, wine ' have passed Guangxi Zhuang Autonomous Region crop varietal approval committee.For many years, through the research that expert deepens continuously, overcome a lot of difficult problems in downy grape cultivation, really achieve downy grape and planted by the wild family that moves towards.
Downy grape seedling-wood breeding adopts usually " three steps heel in seedling method ", and concrete operation method is as follows: get spray to the hermaphrodite flower strain in kind garden and carry out group training; Plantlet in vitro is heeled in, and matrix is that 80% red soil+20% is husky; Hermaphrodite flower seedlings of hairy grape from tissue culture, through heeling in, after growing to certain altitude, gets its epicormic branch cuttage; Plantlet in vitro after cuttage, defines complete root system and blade, can take to nutrition cup and cultivate.Nutrition cup conventional is at present plastic material, but the gas permeability of plastic nutritional cup, melt water and to melt soil nature all undesirable, in nutrition cup cultivating process, often occur that matrix hardens, the root system of grape forms nest root, rare, the problem such as rotten root because penetrating chamber wall, the serious survival rate reducing nursery stock.Cause the resistance of grapevine seedling and growth rate to reduce simultaneously, also significant impact is produced to grapevine seedling growth from now on.When transplanting, also needing plastic nutritional cup to remove, increasing work load, affecting efficiency.After the nutrition cup of plastics abandons, owing to being not easily decomposed, also to environment.Pure pond sludge is often selected in the selection of matrix, owing to excessively hardening, heel in survival rate lower, pure red soil is that matrix also has similar phenomenon, be that matrix is loose ventilative with sand, survival rate increases, but the poor nutritional of matrix can not provide growth required nutrient in time, and matrix water retention capacity is weak, make the seedling growing way that survives slow or not good in middle and later periods growth.
Therefore, how to select the nutrition cup that is applicable to and matrix of planting, thus improve the survival rate of downy grape seedling, resistance and growth rate, become industry problem demanding prompt solution.
Summary of the invention
The technical problem to be solved in the present invention is a kind of method providing seedlings of hairy grape from tissue culture nutrition cup to heel in, and to improve the survival rate of downy grape nursery stock, plant resistance and growth rate, reduces seedling cost, reduces labour intensity.
For solving the problems of the technologies described above, the invention provides following technical scheme:
A kind of seedlings of hairy grape from tissue culture nutrition cup heels in matrix, and by volume percentage comprises following component: red soil 45% ~ 50%, peat 40% ~ 50%, well-rotted farmyard manure 1% ~ 5%.Research points out that its total porosity of material as matrix should in 54% ~ 96% scope, size void ratio 0.25 ~ 0.67 scope, the mixed-matrix total porosity of this proportioning is 62.3%, and size void ratio is 0.3, namely venting capability is better, and moisture holding capacity also meets the condition as matrix.
Preferably, a kind of seedlings of hairy grape from tissue culture nutrition cup heels in matrix, and by volume percentage comprises following component: red soil 50%, peat 48%, well-rotted farmyard manure 2%.
The method that seedlings of hairy grape from tissue culture nutrition cup is heeled in, comprises the steps:
(1) by volume percentage is: matrix heeled in by the proportional arrangement downy grape of red soil 45% ~ 50%, peat 40% ~ 50%, well-rotted farmyard manure 1% ~ 5%, loads non-woven fabrics nutrition cup;
(2) select seedling, choose the plantlet in vitro defining complete root system and blade after cuttage;
(3) heel in, the plantlet in vitro in step (2) is transplanted in the non-woven fabrics nutrition cup in step (1) and heels in.
Preferably, described seedlings of hairy grape from tissue culture nutrition cup is heeled in method step (1), at the upper strata of nutrition cup mesostroma tiling 1cm fine sand.
Preferably, described non-woven fabrics nutrition cup adopts diameter to be 8cm, and the long nonwoven for 12cm is furnished with end container.
The method that seedlings of hairy grape from tissue culture nutrition cup of the present invention is heeled in has following beneficial effect:
1. the present invention heels in process the mixed-matrix adopting red soil+peat+well-rotted farmyard manure at seedlings of hairy grape from tissue culture nutrition cup, this matrix is obviously better than traditional matrices, survival rate of seedling can reach more than 90%, the middle and later periods of seedling growth, still can ensure that nutrient supply is abundant, nursery stock is grown fine, and can meet next step continue adopt fringe action need, light weight be convenient to transport.
2. heel in nutrition cup and select nonwoven cloth material, there is the light transmission of good gas permeability, water penetration and appropriateness, heat insulating ability, it is corrosion-resistant, acid and alkali-resistance, not damage by worms, not hardening at low temperatures, can wash, easily reclaim, storage, light for environmental pollution.To plant nursery stock with it, seedling root is complete, and contributes to forming a large amount of side roots.The seedling root of propelling container wall cuts through air the sturdy callus formed after root, forms the state of saving up strength to start out.Improve the survival rate of downy grape seedling, resistance and growth rate, seedling growth is healthy and strong, and planting survival rate is high, need not remove nutrition cup, can directly plant when planting downy grape, saves labour.
Embodiment
Further describe the present invention referring to embodiment, to make those skilled in the art can implement according to this with reference to specification word, scope is not limited by embodiments of the present invention.
This experiment is divided into experimental group and control group, then observes the survival rate heeled in of nursery stock and growing state.
Control group 1
Matrix is heeled in as downy grape with 100% pond sludge, loading diameter is 8cm, and the long nonwoven for 12cm is furnished with in end nutrition cup, then chooses the plantlet in vitro defining complete root system and blade after cuttage, heel in the nutrition cup that plantlet in vitro is transplanted to, the survival rate that observation nursery stock is heeled in and growing state.
Control group 2
Matrix is heeled in as downy grape with 100% red soil, loading diameter is 8cm, and the long nonwoven for 12cm is furnished with in end nutrition cup, then chooses the plantlet in vitro defining complete root system and blade after cuttage, heel in the nutrition cup that plantlet in vitro is transplanted to, the survival rate that observation nursery stock is heeled in and growing state.
Control group 3
Matrix is heeled in as downy grape with 100% sand, loading diameter is 8cm, and the long nonwoven for 12cm is furnished with in end nutrition cup, then chooses the plantlet in vitro defining complete root system and blade after cuttage, heel in the nutrition cup that plantlet in vitro is transplanted to, the survival rate that observation nursery stock is heeled in and growing state.
Experimental group 1
According to percent by volume be: matrix heeled in by the proportional arrangement downy grape of red soil 50%, peat 48%, well-rotted farmyard manure 2%, loading diameter is 8cm, the long nonwoven for 12cm is furnished with in end nutrition cup, at the upper strata of nutrition cup mesostroma tiling 1cm fine sand, then the plantlet in vitro defining complete root system and blade after cuttage is chosen, plantlet in vitro is transplanted in nutrition cup and is heeled in, the survival rate that observation nursery stock is heeled in and growing state.
Experimental group 2
According to percent by volume be: matrix heeled in by the proportional arrangement downy grape of red soil 45%, peat 50%, well-rotted farmyard manure 5%, loading diameter is 8cm, the long nonwoven for 12cm is furnished with in end nutrition cup, at the upper strata of nutrition cup mesostroma tiling 1cm fine sand, then the plantlet in vitro defining complete root system and blade after cuttage is chosen, plantlet in vitro is transplanted in nutrition cup and is heeled in, the survival rate that observation nursery stock is heeled in and growing state.
Experimental group 3
According to percent by volume be: matrix heeled in by the proportional arrangement downy grape of red soil 50%, peat 48%, well-rotted farmyard manure 2%, loading diameter is 8cm, the long plastics for 12cm have in end nutrition cup, at the upper strata of nutrition cup mesostroma tiling 1cm fine sand, then the plantlet in vitro defining complete root system and blade after cuttage is chosen, plantlet in vitro is transplanted in nutrition cup and is heeled in, the survival rate that observation nursery stock is heeled in and growing state.
Analysis of experimental data
| Experiment | Survival rate (%) | Growing state |
| Control group 1 | ≧65 | Harden, middle and later periods poor growth |
| Control group 2 | ≧80 | Harden, middle and later periods poor growth |
| Control group 3 | ≧90 | Growing way is slow, and middle and later periods growth is not good |
| Experimental group 1 | ≧95 | Middle and later periods grows fine |
| Experimental group 2 | ≧93 | Middle and later periods grows fine |
| Experimental group 3 | ≧90 | Middle and later periods growth is not good |
As can be seen from experimental data, do matrix with pure pond sludge, plant percent reaches 65%, and matrix easily hardens, and nursery stock is in middle and later periods poor growth; Do matrix with pure red soil, plant percent reaches 80%, and matrix easily hardens, and nursery stock is in middle and later periods poor growth; Do matrix with pure sand, plant percent reaches 90%, but the growing way of nursery stock is slow, and middle and later periods growth is not good; With the mixed-matrix of red soil, peat and well-rotted farmyard manure, the survival rate of nursery stock all reaches more than 90%, but when nutrition cup plastic cup is cultivated, nursery stock is not good in the growth of middle and later periods.Comprehensively above-mentioned, use advantage of the present invention to be: to heel in survival rate high, growing fine of middle and later periods nursery stock.
Claims (1)
1. the method heeled in of seedlings of hairy grape from tissue culture nutrition cup, comprises the steps:
By volume percentage is: matrix heeled in by the proportions downy grape of red soil 50%, peat 48%, well-rotted farmyard manure 2%, and loading diameter is 8cm, and the long nonwoven for 12cm is furnished with end nutrition cup, is furnished with the upper strata tiling 1cm fine sand of end nutrition cup mesostroma at nonwoven; Then the plantlet in vitro defining complete root system and blade after cuttage is chosen; The plantlet in vitro obtained is transplanted to nonwoven to be furnished with in end nutrition cup and to heel in.
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| CN201310322317.2A CN103477901B (en) | 2013-07-29 | 2013-07-29 | Nutritive cup provisional-planting substrate and provisional-planting method for tissue culture seedlings of downy grapes |
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| CN103477901B true CN103477901B (en) | 2015-07-15 |
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| CN105265159B (en) * | 2015-11-20 | 2017-12-12 | 广西壮族自治区药用植物园 | Knoxia valerianoide tissue-cultured seedling cultural method |
| CN106937601B (en) * | 2017-04-28 | 2019-09-20 | 福建省农业科学院农业工程技术研究所 | A method of improving grape test tube seedling transplanting survival rate |
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| CN101258836A (en) * | 2008-04-29 | 2008-09-10 | 广西壮族自治区林业科学研究院 | Protruding eucalyptus tissue culture fast reproducing method |
| CN101385435A (en) * | 2008-10-31 | 2009-03-18 | 天津滨海大顺花卉科技发展股份有限公司 | Cuttage method of Schefflera octophylla(Lour.)Harms |
| CN102948310A (en) * | 2012-08-14 | 2013-03-06 | 刘树平 | Method for planting cinnamomum kotoense seedling |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01211429A (en) * | 1988-02-18 | 1989-08-24 | Santomi Sangyo Kk | Method for tissue culture and tissue culture tool used therefor |
| JPH01218518A (en) * | 1988-02-25 | 1989-08-31 | Mitsui Petrochem Ind Ltd | Method of plant tissue culture |
| JP2007282539A (en) * | 2006-04-14 | 2007-11-01 | Oide Takehisa | Production method for lippia nodiflora of verbenacae family |
| WO2011136285A1 (en) * | 2010-04-27 | 2011-11-03 | 日本製紙株式会社 | Cell differentiation promoter and use of same |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0275682A1 (en) * | 1986-12-18 | 1988-07-27 | Mitsui Petrochemical Industries, Ltd. | Method of multiplicating bulbous plants |
| CN101258836A (en) * | 2008-04-29 | 2008-09-10 | 广西壮族自治区林业科学研究院 | Protruding eucalyptus tissue culture fast reproducing method |
| CN101385435A (en) * | 2008-10-31 | 2009-03-18 | 天津滨海大顺花卉科技发展股份有限公司 | Cuttage method of Schefflera octophylla(Lour.)Harms |
| CN102948310A (en) * | 2012-08-14 | 2013-03-06 | 刘树平 | Method for planting cinnamomum kotoense seedling |
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| 野生毛葡萄两性花优良株系种苗繁育技术研究;邹瑜等;《中国南方果树》;20110631;第40卷(第3期);第76-78页 * |
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