CN103460041B - 白细胞的分类计数方法、白细胞分类试剂盒及白细胞分类试剂 - Google Patents

白细胞的分类计数方法、白细胞分类试剂盒及白细胞分类试剂 Download PDF

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CN103460041B
CN103460041B CN201280016125.2A CN201280016125A CN103460041B CN 103460041 B CN103460041 B CN 103460041B CN 201280016125 A CN201280016125 A CN 201280016125A CN 103460041 B CN103460041 B CN 103460041B
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reagent
organic acid
concentration
aromatic organic
leukocytes
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CN103460041A (zh
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大塚佐绪里
八町和树
水上利洋
小国振一郎
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Sysmex Corp
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/011—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells with lysing, e.g. of erythrocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
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  • Molecular Biology (AREA)
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  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
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  • Ecology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Signal Processing (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201280016125.2A 2011-04-28 2012-04-18 白细胞的分类计数方法、白细胞分类试剂盒及白细胞分类试剂 Active CN103460041B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2011101595A JP5583629B2 (ja) 2011-04-28 2011-04-28 白血球の分類計数方法、白血球分類試薬キット及び白血球分類試薬
JP2011-101595 2011-04-28
PCT/JP2012/060432 WO2012147578A1 (ja) 2011-04-28 2012-04-18 白血球の分類計数方法、白血球分類試薬キット及び白血球分類試薬

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CN103460041B true CN103460041B (zh) 2015-04-01

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US (2) US20140120530A1 (enExample)
EP (1) EP2703812B1 (enExample)
JP (1) JP5583629B2 (enExample)
CN (1) CN103460041B (enExample)
BR (1) BR112013027349B1 (enExample)
SG (1) SG194452A1 (enExample)
WO (1) WO2012147578A1 (enExample)

Families Citing this family (10)

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Publication number Priority date Publication date Assignee Title
US10208346B2 (en) * 2013-04-19 2019-02-19 Epiontis Gmbh Method for identifying the quantitative cellular composition in a biological sample
JP6461494B2 (ja) 2014-06-19 2019-01-30 シスメックス株式会社 血液分析装置、血液分析方法及び血液分析プログラム
JP6383216B2 (ja) * 2014-08-08 2018-08-29 シスメックス株式会社 血液分析方法、血液分析装置およびプログラム
JP6238856B2 (ja) 2014-08-25 2017-11-29 シスメックス株式会社 尿中異型細胞の分析方法、尿分析装置および体液中異型細胞の分析方法
CN108318408B (zh) * 2018-01-30 2020-04-17 深圳唯公生物科技有限公司 白细胞分类用样本的预处理试剂及方法
WO2020042027A1 (zh) * 2018-08-29 2020-03-05 深圳迈瑞生物医疗电子股份有限公司 血液样本检测的方法、血液样本检测仪和存储介质
CN109238801B (zh) * 2018-10-19 2021-08-13 武汉百合龙腾生物科技有限责任公司 一种血细胞分析用溶血剂
JP7357425B2 (ja) * 2020-03-19 2023-10-06 シスメックス株式会社 細胞分類方法、分類装置及びプログラム。
JP7293269B2 (ja) * 2021-02-22 2023-06-19 シスメックス株式会社 溶血試薬、試薬キットおよび白血球の分類方法
BR102023003038A2 (pt) 2022-03-17 2024-01-02 Sysmex Corporation Método para classificar glóbulos brancos em subpopulações

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0882983A2 (en) * 1997-05-19 1998-12-09 Toa Medical Electronics Co., Ltd. Reagent and method for classification and counting of leukocytes
CN101097181A (zh) * 2006-06-26 2008-01-02 希森美康株式会社 试样分析用试剂、试样分析用试剂盒及试样分析方法
CN101470108A (zh) * 2007-12-24 2009-07-01 深圳迈瑞生物医疗电子股份有限公司 一种对白细胞进行分类的试剂和方法
CN101750476A (zh) * 2008-12-08 2010-06-23 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3467310B2 (ja) 1994-04-21 2003-11-17 シスメックス株式会社 白血球分析用試薬及び白血球の分類方法
JP4484375B2 (ja) * 2001-01-10 2010-06-16 シスメックス株式会社 異常細胞検出方法
JP4248017B2 (ja) * 2005-10-28 2009-04-02 シスメックス株式会社 白血球分類計数方法及び白血球分類計数試薬キット
CN101349644B (zh) 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
US8802025B2 (en) * 2007-09-27 2014-08-12 Sysmex Corporation Reagent kit for sample analysis and sample analysis method
CN101750274B (zh) * 2008-12-17 2014-06-25 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0882983A2 (en) * 1997-05-19 1998-12-09 Toa Medical Electronics Co., Ltd. Reagent and method for classification and counting of leukocytes
CN101097181A (zh) * 2006-06-26 2008-01-02 希森美康株式会社 试样分析用试剂、试样分析用试剂盒及试样分析方法
CN101470108A (zh) * 2007-12-24 2009-07-01 深圳迈瑞生物医疗电子股份有限公司 一种对白细胞进行分类的试剂和方法
CN101750476A (zh) * 2008-12-08 2010-06-23 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法

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Publication number Publication date
SG194452A1 (en) 2013-12-30
JP5583629B2 (ja) 2014-09-03
JP2012233754A (ja) 2012-11-29
EP2703812A1 (en) 2014-03-05
EP2703812A4 (en) 2014-12-10
US20200326332A1 (en) 2020-10-15
US12072330B2 (en) 2024-08-27
CN103460041A (zh) 2013-12-18
US20140120530A1 (en) 2014-05-01
BR112013027349B1 (pt) 2021-05-18
EP2703812B1 (en) 2018-02-28
BR112013027349A2 (pt) 2017-01-17
WO2012147578A1 (ja) 2012-11-01

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