CN103354835B - 抑制人多能干细胞生长的试剂和方法 - Google Patents

抑制人多能干细胞生长的试剂和方法 Download PDF

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Publication number
CN103354835B
CN103354835B CN201080071181.7A CN201080071181A CN103354835B CN 103354835 B CN103354835 B CN 103354835B CN 201080071181 A CN201080071181 A CN 201080071181A CN 103354835 B CN103354835 B CN 103354835B
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cells
cell
pluripotent stem
differentiation
population
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CN103354835A (zh
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托马斯·斯库尔兹
艾伦·罗宾斯
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Viacyte Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
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  • Microbiology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Gynecology & Obstetrics (AREA)
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  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CN201080071181.7A 2010-12-08 2010-12-08 抑制人多能干细胞生长的试剂和方法 Expired - Fee Related CN103354835B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2010/059586 WO2012078153A1 (en) 2010-12-08 2010-12-08 Agents and methods for inhibiting human pluripotent stem cell growth

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CN103354835A CN103354835A (zh) 2013-10-16
CN103354835B true CN103354835B (zh) 2016-03-02

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US (2) US9365830B2 (enExample)
EP (1) EP2649177B1 (enExample)
JP (1) JP5875597B2 (enExample)
CN (1) CN103354835B (enExample)
AU (1) AU2010365057B2 (enExample)
CA (1) CA2820419A1 (enExample)
ES (1) ES2690663T3 (enExample)
WO (1) WO2012078153A1 (enExample)

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US20140234963A1 (en) 2011-06-21 2014-08-21 Novo Nordisk A/S Efficient induction of definitive endoderm from pluripotent stem cells
EP2980207B1 (en) * 2013-03-25 2018-12-05 Foundation for Biomedical Research and Innovation at Kobe Cell sorting method
KR102229266B1 (ko) * 2013-05-30 2021-03-19 아지노모토 가부시키가이샤 줄기 세포의 배양용 배지
ES2776707T3 (es) * 2013-08-23 2020-07-31 Sumitomo Chemical Co Método para producir tejido retiniano y células relacionadas con la retina
EP3097186B1 (en) 2014-01-21 2019-05-08 The Medical College of Wisconsin, Inc. Methods for selective inhibition of pluripotent stem cells
JP6197947B2 (ja) * 2014-03-31 2017-09-20 味の素株式会社 幹細胞用培地
CA2994192C (en) * 2015-08-13 2020-06-16 Beihao Stem Cell And Regenerative Medicine Research Institute Co., Ltd. Induced extended pluripotent stem cells, methods of making and using
CA3000742A1 (en) 2015-10-02 2017-04-06 Silver Creek Pharmaceuticals, Inc. Bi-specific therapeutic proteins for tissue repair
GB2562406B (en) 2016-01-12 2020-09-02 Cedars Sinai Medical Center A method of non destructive monitoring of biological processes in microfluidic tissue culture systems
WO2018140647A1 (en) * 2017-01-25 2018-08-02 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells
WO2018139600A1 (ja) * 2017-01-27 2018-08-02 株式会社カネカ 内胚葉系細胞集団、及び多能性細胞から三胚葉のいずれかの細胞集団を製造する方法
JP2020509759A (ja) * 2017-03-07 2020-04-02 プレートレット バイオジェネシス， インコーポレイテッド 再循環バイオリアクタ
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
EP3569693A4 (en) 2017-06-05 2020-11-18 Terumo Kabushiki Kaisha CELL CULTURE MANUFACTURING PROCESS
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
CN108570443A (zh) * 2017-12-05 2018-09-25 皓昇莱生物制药有限公司 一种用于培养尿液来源细胞的培养基
WO2019183597A1 (en) 2018-03-23 2019-09-26 Cedars-Sinai Medical Center Methods of use of islet cells
JP7016185B2 (ja) * 2018-03-28 2022-02-04 国立大学法人大阪大学 幹細胞由来涙腺組織の作製方法
US11981918B2 (en) 2018-04-06 2024-05-14 Cedars-Sinai Medical Center Differentiation technique to generate dopaminergic neurons from induced pluripotent stem cells
JP7464532B2 (ja) 2018-04-06 2024-04-09 シーダーズ－サイナイ メディカル センター マイクロ流体チップ上のヒト多能性幹細胞由来の神経変性疾患モデル
CN110684714B (zh) * 2018-07-05 2023-05-09 广东博溪生物科技有限公司 一种屏障功能弱化模型的构建方法
MX2021000743A (es) 2018-07-19 2021-07-15 Platelet Biogenesis Inc Biorreactor de recirculacion apilado.
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
TWI862513B (zh) * 2018-09-19 2024-11-21 日商千紙鶴治療公司 包含胰島素產生細胞之細胞集團、包含該細胞集團之醫藥、其用途及製造方法
JP7781522B2 (ja) 2018-09-27 2025-12-08 国立大学法人大阪大学 多能性幹細胞由来細胞のシート化方法
JP7409670B2 (ja) * 2018-12-21 2024-01-09 国立大学法人北海道大学 多能性幹細胞を除去するための組成物、及び多能性幹細胞の除去方法
CN110373375A (zh) * 2019-03-19 2019-10-25 杭州原生生物科技有限公司 一种制备纤维细胞作为细胞培养基的方法和用途
JP7753197B2 (ja) 2019-09-05 2025-10-14 クリスパー セラピューティクス アクチェンゲゼルシャフト ユニバーサルドナー細胞
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JP2014501518A (ja) 2014-01-23
CA2820419A1 (en) 2012-06-14
WO2012078153A1 (en) 2012-06-14
EP2649177A1 (en) 2013-10-16
EP2649177B1 (en) 2018-09-05
US9365830B2 (en) 2016-06-14
JP5875597B2 (ja) 2016-03-02
US20130280802A1 (en) 2013-10-24
CN103354835A (zh) 2013-10-16
ES2690663T3 (es) 2018-11-21
US20160281058A1 (en) 2016-09-29
EP2649177A4 (en) 2014-04-23
AU2010365057B2 (en) 2016-12-22
AU2010365057A1 (en) 2013-06-20

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