CN103262868B - Biological insecticide for cherry trees and preparation method thereof - Google Patents

Biological insecticide for cherry trees and preparation method thereof Download PDF

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Publication number
CN103262868B
CN103262868B CN201310193160.8A CN201310193160A CN103262868B CN 103262868 B CN103262868 B CN 103262868B CN 201310193160 A CN201310193160 A CN 201310193160A CN 103262868 B CN103262868 B CN 103262868B
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extract
garlic
ginger
extractive
preparation
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CN103262868A (en
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王万枝
李狗拉
张克俭
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LUOYANG ZAOCHUN LARGE CHERRY PLANTATION FARMER SPECIALIZED COOPERATIVES
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LUOYANG ZAOCHUN LARGE CHERRY PLANTATION FARMER SPECIALIZED COOPERATIVES
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Abstract

The invention provides a biological insecticide for cherry trees and a preparation method of the biological insecticide. Raw materials comprise celastrus angulatus, a fresh ginger extractive, a garlic extractive, an azedarach extractive, a rhizoma polygonati extractive and a wild chrysanthemum flower extractive, wherein the mass percentage of the adding amount of each raw material is as follows: 15-20% of the celastrus angulatus, 10-30% of the fresh ginger extractive, 10-18% of the garlic extractive, 17-25% of the azedarach extractive, 10-17% of the rhizoma polygonati extractive and 2-8% of the wild chrysanthemum flower extractive. The prepared biological insecticide for the cherry trees has very good insecticidal effect on leaf miners, red spiders, white spiders and pseudaulacaspis pentagona of the cherry trees; and the pesticide effect is 10-15 days and the insect-killing rate is up to more than 95%. The prepared biological insecticide for the cherry trees disclosed by the invention is applied during a flower and fruit period and cherry fruits are completely non-toxic and harmless and can be eaten at ease.

Description

A kind of biological insecticides for cherry tree and preparation method thereof
Technical field
The present invention relates to a kind of biological insecticides and preparation method thereof, specifically a kind of biological insecticides for cherry tree and preparation method thereof.
Background technology
Cherry tree Armeniaca Mume of Rosaceae belongs to defoliation small arbor, Gao Keda 8m.Leaf is avette oval to ovum shape, long 7 ~ 12cm, the sharp point of tip, base portion is circular, the edge heavy sawtooth that differs in size, between cog has gland, above without hair or micro-hairiness, raw pubescence is dredged at the back side.Floral white, footpath is 1.5 ~ 2.5cm approximately, calyx tube hairiness; 3 ~ 6 cluster into raceme.Fruit is subsphaeroidal, footpath 1 ~ 1.5cm, redness.In April at florescence, first leaf is open; Fruit is ripe 5 ~ June.Easily there is the insect pests such as two spotted spider mite, aphid, white peach scale in this phase in late April ~ May in cherry tree, should strengthen the extermination of disease and insect pest.
Biological insecticides are mainly divided into bacillus thuringiensis,Bt, insect viruses, the large class of plant leaching liquor 3, have and draw materials conveniently, with low cost, the control phase is long, efficient, economical, safe, pollution-free, the feature such as compatible with environment high is that the best agricultural chemicals that current production nuisanceless green vegetables produce is selected.
Summary of the invention
The present invention seeks to the deficiency for solving the problems of the technologies described above, a kind of biological insecticides for cherry tree and preparation method thereof are provided, it has efficiently, the advantage such as economic, safe, pollution-free.
A kind of biological insecticides for cherry tree, its raw material is comprised of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 15% ~ 25%, Ginger P.E 10% ~ 30%, garlic P.E 10% ~ 18%, Cortex Meliae extract 17% ~ 25%, Rhizoma Polygonati extract 10% ~ 17%, Flos Chrysanthemi Indici extract 2% ~ 8%.
For biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, after cleaning, drying, take 300 g, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, then centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge, get supernatant after 0.25 μ m bacterium membrane filtration, obtain garlic or Ginger P.E, be placed in 4 ℃ of refrigerators and save backup;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Cortex Meliae extract, is cooled to room temperature standby;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Rhizoma Polygonati extract, is cooled to room temperature standby;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Flos Chrysanthemi Indici extract, is cooled to room temperature standby;
Step 5, according to aforementioned proportion, get successively prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract and be placed in container, after fully mixing, obtain mixture I;
Step 6, in step 5 gained mixture I, add successively the Celastrus angulatus of aforementioned proportion and prepared garlic P.E, after mixing, gained mixture II is the present invention for the biological insecticides of cherry tree, is placed on room temperature preservation, standby.
Beneficial effect
1, the present invention is for the biological insecticides of cherry tree, and its constituent Celastrus angulatus has insect is had to anesthesia, food refusal and stomach toxicity, action of contace poison, and in conjunction with the insecticidal effect of Cortex Meliae extract, both acting in conjunction effects are more obvious; Ginger and the garlic P.E itself with sterilization and anthelminthic effect of take is bleeding agent, booster action in conjunction with Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, the present invention reaches good insecticidal effect for each component perfect adaptation of biological insecticides of cherry tree, and it all produces effect to insects such as leaf miner, two spotted spider mite, white spider, white peach scales.
2, the present invention is for the biological insecticides of cherry tree, its preparation method is simple, with low cost, the onset that the prepared biological insecticides for cherry tree have overcome general plant resource and biological insecticides slowly, without the effect of tagging, easily photodissociation, oxidizable shortcoming; There is stomach toxicity, the transdermal speed that general plant resource and biological insecticides do not possess and kill (tagging), fumigation, drive, food refusal, the multiaction of anesthesia, special standby is that cherry tree leaf miner, two spotted spider mite, white spider, white peach scale are had to good insecticidal effect.
3, non-environmental-pollution, the present invention is for the biological insecticides of cherry tree, from can be oral, the Chinese herbal medicine of external application is to the nontoxic effect of people and animals, meet middle Country Chinese herbal medicine voluminous dictionary and include medication.Cherry tree, at flowering fruit bearing stage, is in general March ~ June, and the insect pests such as two spotted spider mite, aphid, white peach scale, leaf miner easily occur, during spray the present invention for the biological insecticides of cherry tree, effect is best, drug effect is long 15 ~ 20 days, killing rate is up to more than 95%.Flowering fruit bearing stage sprays the present invention for the biological insecticides of cherry tree, and cherry fruit totally nontoxic is harmless, can relievedly eat.
Embodiment
A kind of biological insecticides for cherry tree, its raw material is comprised of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 15% ~ 25%, Ginger P.E 10% ~ 30%, garlic P.E 10% ~ 18%, Cortex Meliae extract 17% ~ 25%, Rhizoma Polygonati extract 10% ~ 17%, Flos Chrysanthemi Indici extract 2% ~ 8%.
For biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, after cleaning, drying, take 300 g, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, then centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge, get supernatant after 0.25 μ m bacterium membrane filtration, obtain garlic or Ginger P.E, be placed in 4 ℃ of refrigerators and save backup;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Cortex Meliae extract, is cooled to room temperature standby;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Rhizoma Polygonati extract, is cooled to room temperature standby;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Flos Chrysanthemi Indici extract, is cooled to room temperature standby;
Step 5, according to aforementioned proportion, get successively prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract and be placed in container, after fully mixing, obtain mixture I;
Step 6, in step 5 gained mixture I, add successively the Celastrus angulatus of aforementioned proportion and prepared garlic P.E, after mixing, gained mixture II is the present invention for the biological insecticides of cherry tree, is placed on room temperature preservation, standby.
Step 7, every 10 cherry trees, using the present invention is 100ml for the dosage of the biological insecticides of cherry tree, adds water 50 ~ 60L, fully shakes up, and cherry tree is carried out to complete stool spray mist to tear drop.
Medication points for attention: should select dusk or the cloudy dispenser of warm, wet weather, and forbid and bactericide, the alkaline agricultural chemicals same period or composite use.The present invention should not be long placed in for the biological insecticides of cherry tree and place, should be now with the current, in order to avoid reduce drug effect.
embodiment 1
A kind of biological insecticides for cherry tree, its raw material is comprised of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 15%, Ginger P.E 26%, garlic P.E 10%, Cortex Meliae extract 24%, Rhizoma Polygonati extract 17%, Flos Chrysanthemi Indici extract 8%.
For biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, after cleaning, drying, take 300 g, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, then centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge, get supernatant after 0.25 μ m bacterium membrane filtration, obtain garlic or Ginger P.E, be placed in 4 ℃ of refrigerators and save backup;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Cortex Meliae extract, is cooled to room temperature standby;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Rhizoma Polygonati extract, is cooled to room temperature standby;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Flos Chrysanthemi Indici extract, is cooled to room temperature standby;
Step 5, according to aforementioned proportion, get successively prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract and be placed in container, after fully mixing, obtain mixture I;
Step 6, in step 5 gained mixture I, add successively the Celastrus angulatus of aforementioned proportion and prepared garlic P.E, after mixing, gained mixture II is the present invention for the biological insecticides of cherry tree, is placed on room temperature preservation, standby.
Step 7, every 10 cherry trees, using the present invention is 100ml for the dosage of the biological insecticides of cherry tree, adds water 60L, fully shakes up, and cherry tree is carried out to complete stool spray mist to tear drop.
Result shows: after the present invention sprays cherry tree for the biological insecticides of cherry tree, its killing rate reaches 95%, and drug effect is grown 15 days.
embodiment 2
A kind of biological insecticides for cherry tree, its raw material is comprised of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 25%, Ginger P.E 10%, garlic P.E 18%, Cortex Meliae extract 25%, Rhizoma Polygonati extract 15%, Flos Chrysanthemi Indici extract 7%.
For biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, after cleaning, drying, take 300 g, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, then centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge, get supernatant after 0.25 μ m bacterium membrane filtration, obtain garlic or Ginger P.E, be placed in 4 ℃ of refrigerators and save backup;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Cortex Meliae extract, is cooled to room temperature standby;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Rhizoma Polygonati extract, is cooled to room temperature standby;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Flos Chrysanthemi Indici extract, is cooled to room temperature standby;
Step 5, according to aforementioned proportion, get successively prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract and be placed in container, after fully mixing, obtain mixture I;
Step 6, in step 5 gained mixture I, add successively the Celastrus angulatus of aforementioned proportion and prepared garlic P.E, after mixing, gained mixture II is the present invention for the biological insecticides of cherry tree, is placed on room temperature preservation, standby.
Step 7, every 10 cherry trees, using the present invention is 100ml for the dosage of the biological insecticides of cherry tree, adds water 50L, fully shakes up, and cherry tree is carried out to complete stool spray mist to tear drop.
Result shows: after the present invention sprays cherry tree for the biological insecticides of cherry tree, its killing rate reaches 97%, and drug effect is grown 20 days.
embodiment 3
A kind of biological insecticides for cherry tree, its raw material is comprised of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 24%, Ginger P.E 30%, garlic P.E 17%, Cortex Meliae extract 17%, Rhizoma Polygonati extract 10%, Flos Chrysanthemi Indici extract 2%.
For biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, after cleaning, drying, take 300 g, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, then centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge, get supernatant after 0.25 μ m bacterium membrane filtration, obtain garlic or Ginger P.E, be placed in 4 ℃ of refrigerators and save backup;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Cortex Meliae extract, is cooled to room temperature standby;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Rhizoma Polygonati extract, is cooled to room temperature standby;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Flos Chrysanthemi Indici extract, is cooled to room temperature standby;
Step 5, according to aforementioned proportion, get successively prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract and be placed in container, after fully mixing, obtain mixture I;
Step 6, in step 5 gained mixture I, add successively the Celastrus angulatus of aforementioned proportion and prepared garlic P.E, after mixing, gained mixture II is the present invention for the biological insecticides of cherry tree, is placed on room temperature preservation, standby.
Step 7, every 10 cherry trees, using the present invention is 100ml for the dosage of the biological insecticides of cherry tree, adds water 56L, fully shakes up, and cherry tree is carried out to complete stool spray mist to tear drop.
Result shows: after the present invention sprays cherry tree for the biological insecticides of cherry tree, its killing rate reaches 96%, and drug effect is grown 18 days.

Claims (1)

1. biological insecticides for cherry tree, it is characterized in that: its raw material is comprised of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, the mass percent of each raw material addition is: Celastrus angulatus 15% ~ 25%, Ginger P.E 10% ~ 30%, garlic P.E 10% ~ 18%, Cortex Meliae extract 17% ~ 25%, Rhizoma Polygonati extract 10% ~ 17%, Flos Chrysanthemi Indici extract 2% ~ 8%; The described process for preparing bio-insecticide for cherry tree, its concrete preparation process is:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, after cleaning, drying, take 300 g, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, with aseptic water washing, be placed in tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, then centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge, get supernatant after 0.25 μ m bacterium membrane filtration, obtain garlic or Ginger P.E, be placed in 4 ℃ of refrigerators and save backup;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Cortex Meliae extract, is cooled to room temperature standby;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Rhizoma Polygonati extract, is cooled to room temperature standby;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after cleaning, drying, add 1L water, be heated to after boiling, slow fire boils truce in 30 minutes, and after filtration, gained supernatant is Flos Chrysanthemi Indici extract, is cooled to room temperature standby;
Step 5, according to the mass percent of each raw material addition of the above-mentioned biological insecticides for cherry tree, get successively prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract and be placed in container, after fully mixing, obtain mixture I;
Step 6, in step 5 gained mixture I, according to the mass percent of each raw material addition of the above-mentioned biological insecticides for cherry tree, add successively Celastrus angulatus and prepared garlic P.E, after mixing, gained mixture II is the biological insecticides for cherry tree, be placed on room temperature preservation, standby.
CN201310193160.8A 2013-05-23 2013-05-23 Biological insecticide for cherry trees and preparation method thereof Expired - Fee Related CN103262868B (en)

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CN106472603A (en) * 2016-10-14 2017-03-08 柳州环山科技有限公司 A kind of biological pesticide
CN106472614A (en) * 2016-10-14 2017-03-08 柳州环山科技有限公司 NEW TYPE OF COMPOSITE biological organic agricultural chemical
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Publication number Priority date Publication date Assignee Title
CN106472603A (en) * 2016-10-14 2017-03-08 柳州环山科技有限公司 A kind of biological pesticide
CN106472614A (en) * 2016-10-14 2017-03-08 柳州环山科技有限公司 NEW TYPE OF COMPOSITE biological organic agricultural chemical
CN106472602A (en) * 2016-10-14 2017-03-08 柳州环山科技有限公司 A kind of composite environment-friendly biological pesticide

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