CN103262868A - Biological insecticide for cherry trees and preparation method thereof - Google Patents
Biological insecticide for cherry trees and preparation method thereof Download PDFInfo
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Abstract
The invention provides a biological insecticide for cherry trees and a preparation method of the biological insecticide. Raw materials comprise celastrus angulatus, a fresh ginger extractive, a garlic extractive, an azedarach extractive, a rhizoma polygonati extractive and a wild chrysanthemum flower extractive, wherein the mass percentage of the adding amount of each raw material is as follows: 15-20% of the celastrus angulatus, 10-30% of the fresh ginger extractive, 10-18% of the garlic extractive, 17-25% of the azedarach extractive, 10-17% of the rhizoma polygonati extractive and 2-8% of the wild chrysanthemum flower extractive. The prepared biological insecticide for the cherry trees has very good insecticidal effect on leaf miners, red spiders, white spiders and pseudaulacaspis pentagona of the cherry trees; and the pesticide effect is 10-15 days and the insect-killing rate is up to more than 95%. The prepared biological insecticide for the cherry trees disclosed by the invention is applied during a flower and fruit period and cherry fruits are completely non-toxic and harmless and can be eaten at ease.
Description
Technical field
The present invention relates to a kind of biological insecticides and preparation method thereof, specifically a kind of biological insecticides for cherry tree and preparation method thereof.
Background technology
Cherry tree rose family plum belongs to defoliation small arbor, Gao Keda 8m.Leaf is avette to ovum shape ellipse, long 7 ~ 12cm, and the sharp point of tip, the base portion circle, edge differs in size and weighs sawtooth, and between cog has gland, does not have hair or little hairiness above, and the back side is dredged and is given birth to pubescence.Floral white, the about 1.5 ~ 2.5cm in footpath, calyx tube hairiness; 3 ~ 6 cluster into raceme.Fruit is subsphaeroidal, footpath 1 ~ 1.5cm, redness.In April at florescence, first leaf is open; Fruit maturation in 5 ~ June.Insect pests such as two spotted spider mite, aphid, white peach scale easily take place in this phase in late April ~ May in cherry tree, should strengthen the extermination of disease and insect pest.
Biological insecticides mainly are divided into bacillus thuringiensis,Bt, insect viruses, plant leaching liquor 3 big classes, have and draw materials conveniently, with low cost, the control phase is long, and is efficient, economical, safe, pollution-free, characteristics such as compatible with environment high are that the best agricultural chemicals that current production nuisanceless green vegetables produce is selected.
Summary of the invention
The present invention seeks to the deficiency for solving the problems of the technologies described above, a kind of biological insecticides for cherry tree and preparation method thereof are provided, it has efficiently, advantage such as economic, safe, pollution-free.
A kind of biological insecticides for cherry tree, its raw material is made up of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 15% ~ 25%, Ginger P.E 10% ~ 30%, garlic P.E 10% ~ 18%, Cortex Meliae extract 17% ~ 25%, Rhizoma Polygonati extract 10% ~ 17%, Flos Chrysanthemi Indici extract 2% ~ 8%.
A kind of biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, take by weighing 300 g after cleaning, drying, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in the tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge then, get supernatant behind 0.25 μ m bacterium membrane filtration, namely obtain garlic or Ginger P.E, it is standby to place 4 ℃ of refrigerators to preserve;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Cortex Meliae extract, it is standby that it is cooled to room temperature;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Rhizoma Polygonati extract, it is standby that it is cooled to room temperature;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Flos Chrysanthemi Indici extract, it is standby that it is cooled to room temperature;
Step 5, get prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract successively according to aforementioned proportion and place container, after fully mixing, obtain the mixture I;
Step 6, add the Celastrus angulatus of aforementioned proportion and prepared garlic P.E successively in step 5 gained mixture I, after mixing, gained mixture II is the biological insecticides that the present invention is used for cherry tree, is placed on room temperature preservation, and is standby.
Beneficial effect
1, the present invention is used for the biological insecticides of cherry tree, and its constituent Celastrus angulatus has insect is had anesthesia, food refusal and stomach toxicity, action of contace poison, and in conjunction with the insecticidal effect of Cortex Meliae extract, both acting in conjunction effects are more obvious; Be bleeding agent with the ginger and the garlic P.E that itself have sterilization and anthelminthic effect, booster action in conjunction with Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, each component perfect adaptation of biological insecticides that the present invention is used for cherry tree reaches insecticidal effect preferably, and it all produces effect to insects such as leaf miner, two spotted spider mite, white spider, white peach scales.
2, the present invention is used for the biological insecticides of cherry tree, its preparation method is simple, with low cost, the onset that the prepared biological insecticides that are used for cherry tree have overcome general plant resource and biological insecticides slowly, do not have the effect of tagging, easily photodissociation, an easy oxidation shortcoming; Have stomach toxicity, the transdermal speed that general plant resource and biological insecticides do not possess and kill (tagging), fumigation, drive, food refusal, the multiaction of anesthesia, the spy has good insecticidal effect to cherry tree leaf miner, two spotted spider mite, white spider, white peach scale.
3, non-environmental-pollution, the present invention is used for the biological insecticides of cherry tree, from can be oral, the Chinese herbal medicine of external application is to the nontoxic effect of people and animals, Country Chinese herbal medicine voluminous dictionary is included medication in meeting.In general cherry tree is March ~ June at flowering fruit bearing stage, and insect pests such as two spotted spider mite, aphid, white peach scale, leaf miner easily take place, during spray the biological insecticides that the present invention is used for cherry tree, effect is best, long 15 ~ 20 days of drug effect, killing rate is up to more than 95%.Flowering fruit bearing stage sprays the biological insecticides that the present invention is used for cherry tree, and the cherry fruit totally nontoxic is harmless, can relievedly eat.
Embodiment
A kind of biological insecticides for cherry tree, its raw material is made up of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 15% ~ 25%, Ginger P.E 10% ~ 30%, garlic P.E 10% ~ 18%, Cortex Meliae extract 17% ~ 25%, Rhizoma Polygonati extract 10% ~ 17%, Flos Chrysanthemi Indici extract 2% ~ 8%.
A kind of biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, take by weighing 300 g after cleaning, drying, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in the tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge then, get supernatant behind 0.25 μ m bacterium membrane filtration, namely obtain garlic or Ginger P.E, it is standby to place 4 ℃ of refrigerators to preserve;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Cortex Meliae extract, it is standby that it is cooled to room temperature;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Rhizoma Polygonati extract, it is standby that it is cooled to room temperature;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Flos Chrysanthemi Indici extract, it is standby that it is cooled to room temperature;
Step 5, get prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract successively according to aforementioned proportion and place container, after fully mixing, obtain the mixture I;
Step 6, add the Celastrus angulatus of aforementioned proportion and prepared garlic P.E successively in step 5 gained mixture I, after mixing, gained mixture II is the biological insecticides that the present invention is used for cherry tree, is placed on room temperature preservation, and is standby.
Step 7, per 10 cherry trees, the dosage that uses the present invention to be used for the biological insecticides of cherry tree is 100ml, adds water 50 ~ 60L, fully shakes up, and cherry tree is carried out complete stool spray mist to tear drop.
Medication points for attention: should select dusk or the cloudy dispenser of warm, wet weather, and forbid and bactericide, the alkaline agricultural chemicals same period or composite use.The biological insecticides that the present invention is used for cherry tree should not put for a long time put put, should be now with the current, in order to avoid reduce drug effect.
Embodiment 1
A kind of biological insecticides for cherry tree, its raw material is made up of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 15%, Ginger P.E 26%, garlic P.E 10%, Cortex Meliae extract 24%, Rhizoma Polygonati extract 17%, Flos Chrysanthemi Indici extract 8%.
A kind of biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, take by weighing 300 g after cleaning, drying, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in the tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge then, get supernatant behind 0.25 μ m bacterium membrane filtration, namely obtain garlic or Ginger P.E, it is standby to place 4 ℃ of refrigerators to preserve;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Cortex Meliae extract, it is standby that it is cooled to room temperature;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Rhizoma Polygonati extract, it is standby that it is cooled to room temperature;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Flos Chrysanthemi Indici extract, it is standby that it is cooled to room temperature;
Step 5, get prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract successively according to aforementioned proportion and place container, after fully mixing, obtain the mixture I;
Step 6, add the Celastrus angulatus of aforementioned proportion and prepared garlic P.E successively in step 5 gained mixture I, after mixing, gained mixture II is the biological insecticides that the present invention is used for cherry tree, is placed on room temperature preservation, and is standby.
Step 7, per 10 cherry trees, the dosage that uses the present invention to be used for the biological insecticides of cherry tree is 100ml, adds water 60L, fully shakes up, and cherry tree is carried out complete stool spray mist to tear drop.
The result shows: the biological insecticides that the present invention is used for cherry tree spray its killing rate of back to cherry tree and reach 95%, long 15 days of drug effect.
Embodiment 2
A kind of biological insecticides for cherry tree, its raw material is made up of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 25%, Ginger P.E 10%, garlic P.E 18%, Cortex Meliae extract 25%, Rhizoma Polygonati extract 15%, Flos Chrysanthemi Indici extract 7%.
A kind of biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, take by weighing 300 g after cleaning, drying, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in the tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge then, get supernatant behind 0.25 μ m bacterium membrane filtration, namely obtain garlic or Ginger P.E, it is standby to place 4 ℃ of refrigerators to preserve;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Cortex Meliae extract, it is standby that it is cooled to room temperature;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Rhizoma Polygonati extract, it is standby that it is cooled to room temperature;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Flos Chrysanthemi Indici extract, it is standby that it is cooled to room temperature;
Step 5, get prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract successively according to aforementioned proportion and place container, after fully mixing, obtain the mixture I;
Step 6, add the Celastrus angulatus of aforementioned proportion and prepared garlic P.E successively in step 5 gained mixture I, after mixing, gained mixture II is the biological insecticides that the present invention is used for cherry tree, is placed on room temperature preservation, and is standby.
Step 7, per 10 cherry trees, the dosage that uses the present invention to be used for the biological insecticides of cherry tree is 100ml, adds water 50L, fully shakes up, and cherry tree is carried out complete stool spray mist to tear drop.
The result shows: the biological insecticides that the present invention is used for cherry tree spray its killing rate of back to cherry tree and reach 97%, long 20 days of drug effect.
Embodiment 3
A kind of biological insecticides for cherry tree, its raw material is made up of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 24%, Ginger P.E 30%, garlic P.E 17%, Cortex Meliae extract 17%, Rhizoma Polygonati extract 10%, Flos Chrysanthemi Indici extract 2%.
A kind of biological insecticides for cherry tree, its concrete steps are:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, take by weighing 300 g after cleaning, drying, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, aseptic water washing is placed in the tissue refiner for 3 times, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge then, get supernatant behind 0.25 μ m bacterium membrane filtration, namely obtain garlic or Ginger P.E, it is standby to place 4 ℃ of refrigerators to preserve;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Cortex Meliae extract, it is standby that it is cooled to room temperature;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Rhizoma Polygonati extract, it is standby that it is cooled to room temperature;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Flos Chrysanthemi Indici extract, it is standby that it is cooled to room temperature;
Step 5, get prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract successively according to aforementioned proportion and place container, after fully mixing, obtain the mixture I;
Step 6, add the Celastrus angulatus of aforementioned proportion and prepared garlic P.E successively in step 5 gained mixture I, after mixing, gained mixture II is the biological insecticides that the present invention is used for cherry tree, is placed on room temperature preservation, and is standby.
Step 7, per 10 cherry trees, the dosage that uses the present invention to be used for the biological insecticides of cherry tree is 100ml, adds water 56L, fully shakes up, and cherry tree is carried out complete stool spray mist to tear drop.
The result shows: the biological insecticides that the present invention is used for cherry tree spray its killing rate of back to cherry tree and reach 96%, long 18 days of drug effect.
Claims (2)
1. biological insecticides that are used for cherry tree, it is characterized in that: its raw material is made up of Celastrus angulatus, Ginger P.E, garlic P.E, Cortex Meliae extract, Rhizoma Polygonati extract and Flos Chrysanthemi Indici extract, and the mass percent of each raw material addition is: Celastrus angulatus 15% ~ 25%, Ginger P.E 10% ~ 30%, garlic P.E 10% ~ 18%, Cortex Meliae extract 17% ~ 25%, Rhizoma Polygonati extract 10% ~ 17%, Flos Chrysanthemi Indici extract 2% ~ 8%.
2. a kind of process for preparing bio-insecticide for cherry tree as claimed in claim 1, it is characterized in that: its concrete preparation process is:
The preparation of step 1, garlic and Ginger P.E: choose fresh garlic or ginger, take by weighing 300 g after cleaning, drying, be put in the ethanol of volumetric concentration 75% and carry out surface sterilization 5 min, be placed in the tissue refiner for 3 times with aseptic water washing, add 100 mL sterile waters, rotating speed homogenate 5 min with 5000r/min are made into pasty state, centrifugal 10min under the condition of 4 ℃ of temperature, rotating speed 10000r/min in centrifuge then, get supernatant behind 0.25 μ m bacterium membrane filtration, namely obtain garlic or Ginger P.E, it is standby to place 4 ℃ of refrigerators to preserve;
The preparation of step 2, Cortex Meliae extract: get azedarach 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Cortex Meliae extract, it is standby that it is cooled to room temperature;
The preparation of step 3, Rhizoma Polygonati extract: get sealwort 230g, remove impurity, smash to pieces after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Rhizoma Polygonati extract, it is standby that it is cooled to room temperature;
The preparation of step 4, Flos Chrysanthemi Indici extract: get mother chrysanthemum 280g, remove impurity, after the cleaning, drying, add 1L water, be heated to boiling after, slow fire boils truce in 30 minutes, after the filtration, the gained supernatant is Flos Chrysanthemi Indici extract, it is standby that it is cooled to room temperature;
Step 5, according to the mass percent of described each the raw material addition of biological insecticides for cherry tree of claim 1, get prepared Ginger P.E, Rhizoma Polygonati extract, Flos Chrysanthemi Indici extract and Cortex Meliae extract successively and place container, after fully mixing, obtain the mixture I;
Step 6, in step 5 gained mixture I, mass percent according to described each the raw material addition of biological insecticides for cherry tree of claim 1, add Celastrus angulatus and prepared garlic P.E successively, after mixing, gained mixture II is the biological insecticides that the present invention is used for cherry tree, be placed on room temperature preservation, standby.
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| CN104026176A (en) * | 2014-06-25 | 2014-09-10 | 无锡市崇安区科技创业服务中心 | Natural organic pesticide composition and preparation method thereof |
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| CN104430659A (en) * | 2014-11-28 | 2015-03-25 | 李晓枫 | Miticide for preventing and treating wheat stone mites of peach trees and preparation method of miticide |
| CN104365707A (en) * | 2014-12-01 | 2015-02-25 | 谢振西 | Preparation method for prevention and treatment of panonychus ulmi |
| CN104522098A (en) * | 2014-12-01 | 2015-04-22 | 谢振西 | Acaricide for controlling panonychus ulmi |
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| CN105076261A (en) * | 2015-07-25 | 2015-11-25 | 安徽博发文化生态园有限公司 | Special pesticide for cherry trees and preparation method therefor |
| CN105211113A (en) * | 2015-08-25 | 2016-01-06 | 蚌埠海上明珠农业科技发展有限公司 | Cherry maturing stage expelling parasite spray and preparation method thereof |
| CN106106569A (en) * | 2016-06-20 | 2016-11-16 | 潜山县富源科技有限公司 | A kind of diseases and pests of tea tree control agent and preparation method thereof |
| CN106259554A (en) * | 2016-08-15 | 2017-01-04 | 卞佳林 | A kind of Magnolia denudata tree method for producing insecticide |
| CN106359485A (en) * | 2016-08-19 | 2017-02-01 | 苏州灵垦农业科技有限公司 | Celangulin-containing biopesticide and preparation method thereof |
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