CN103130549A - Preparation method for selenium-rich nutritional agent capable of improving survival rate of ornamental chrysanthemum tissue culture seedlings - Google Patents
Preparation method for selenium-rich nutritional agent capable of improving survival rate of ornamental chrysanthemum tissue culture seedlings Download PDFInfo
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- CN103130549A CN103130549A CN2013100830366A CN201310083036A CN103130549A CN 103130549 A CN103130549 A CN 103130549A CN 2013100830366 A CN2013100830366 A CN 2013100830366A CN 201310083036 A CN201310083036 A CN 201310083036A CN 103130549 A CN103130549 A CN 103130549A
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Abstract
The invention discloses a preparation method for selenium-rich nutritional agent capable of improving the survival rate of ornamental chrysanthemum tissue culture seedlings. The selenium-rich nutritional agent comprises the following needed materials: selenium ore powder, humic acid, monopotassium phosphate, urea and bacillus subtilis soybean fermentation liquor. The preparation method for the selenium-rich nutritional agent comprises the following steps of: preparing bacillus subtilis soybean fermentation liquor; crushing and activating the selenium ore powder; mixing the prepared bacillus subtilis soybean fermentation liquor with the activated selenium ore powder for cultivating in a container; crushing and adding the monopotassium phosphate and the urea to the container for being vibrated; and adding the humic acid to the container for being uniformly stirred to obtain the selenium-rich nutritional agent. According to the preparation method disclosed by the invention, the nutrient substances needed for the normal growth of the chrysanthemum can be ensured, the survival rate of the ornamental chrysanthemum tissue culture seedlings can be greatly improved to 92.8%. Moreover, the preparation method is simple to operate, low in cost and provided with certain generalizability.
Description
Technical field
The present invention relates to a kind of chrysanthemum training tissue culture seedling required selenium-rich nutritive agent in period that is mainly used in viewing and admiring, be specially the preparation method that the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising.
Background technology
Although the breeding coefficient of tissue cultured seedling is very large, there is following deficiency: 1, blade unprotect tissue, the intercellular substance is large in addition, and pore is opened a business large, so moisture loss is very fast, is easy to wilt.2, root unrooted hair or few, and some root of growing from callus and dredging of bud organize not identical, so water-retaining capacity a little less than, therefore surviving rate is lower.During training tissue culture seedling, with the sand loam of tissue cultured seedling transplanting in sterilising treatment, impose liquid composite fertilizer once every 1～2 week.This type of compound manure can only singlely provide nitrogen, phosphorus, potassium, guarantees the normal growth needs of tissue cultured seedling.Not having a kind of fertilizer on market can be for the growth characteristic of viewing and admiring the chrysanthemum tissue cultured seedling, a kind of fertilizer of making to measure for improving its surviving rate.The selenium-rich nutritive agent mainly adopt the high selenium zone of bestowing favour abundant contain selenium carbonaceous and siliceous shale, become selenium mineral powder by grinding and processing, to carry out science composite with macroelement.Selenium is to crop growth, and to improving photosynthesis, increasing both production and income has certain effect, and report is all arranged at home, yet but there is no report for the surviving rate that improves tissue cultured seedling with selenium.
Summary of the invention
Technical problem solved by the invention is to provide a kind of raising to view and admire the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate, the method has adopted plant rich in selenium nutrition technology, in the hardening phase by essential nutritive element and somatomedin is provided to tissue cultured seedling.Selenium has promotion photosynthesis, improves improving activity of root system, and hestening rooting improves its resistance, and the tissue cultured seedling surviving rate is improved.
In the hardening phase, promote taking root of chrysanthemum tissue cultured seedling, improve photosynthetic efficiency, improve its resistance, thereby improve its surviving rate.
Technical problem solved by the invention realizes by the following technical solutions:
The preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising, according to weight percent, the required raw material of described selenium-rich nutritive agent consists of: selenium mineral powder 5～10%, humic acids 20～25%, potassium primary phosphate 5～10%, urea 5～15%, subtilis bean pulp fermentation liquid 35～40%, and the weight percent sum of above raw material is 100%;
Described preparation method specifically comprises the following steps:
(1) preparation subtilis bean pulp fermentation liquid: raw material is 7～15kg dregs of beans, 18～35kg water, 0.5～1L subtilis bacteria suspension; Concrete steps are: with the actication of culture of subtilis and with the dregs of beans pulverizing, then with both mixed fermentation, namely obtain subtilis bean pulp fermentation liquid;
(2) selenium mineral powder is pulverized and is activated selenium mineral powder;
The selenium mineral powder of the subtilis bean pulp fermentation liquid that (3) step (1) is prepared and step (2) activation is mixed in container to be cultivated;
(4) potassium primary phosphate, urea are pulverized, then joined in said vesse and concussion;
(5) at last humic acids is added in said vesse and stirring and evenly mixing, namely get the selenium-rich nutritive agent.
As preferably, the subtilis bean pulp fermentation liquid described in step (1) is coexisted by solid-state and liquid, forms saturated solution after reaction.
As preferably, in step (1) according to weight ratio, dregs of beans: water: subtilis=15:34:1.
As preferably, step is crushed to 600 orders with dregs of beans in (1), adds 1kg sucrose and 0.15kg potassium primary phosphate powder during preparation bean pulp fermentation liquid.
As preferably, the fermentation condition in step (1) was: 37 ℃ of bottom fermentations 72 hours.
As preferably, step is crushed to 400 orders with selenium mineral powder in (2).
As preferably, the culture condition in step (3) was: rotating speed 250r/min, 37 ℃ of lower constant temperature culture 36 hours.
As preferably, in step (4), potassium primary phosphate, urea are crushed to 400 orders below 70% at relative air humidity, prevent the suction moisture regain.
The present invention utilizes the fermentation of bacillus subtilis technology, make elemental selenium in selenium mineral powder under certain leavening temperature with microbial metabolites generation biochemical reaction, the amino acid that fermentation produces, little peptide and polypeptide and the selenium generation seleno-amino acids that is closely linked is easily carried out active transport by tissue cultured seedling.
Compared with prior art, advantage of the present invention is: the present invention can guarantee the nutrient substance that the normal growth of chrysanthemum is required, can significantly improve the surviving rate of viewing and admiring the chrysanthemum tissue cultured seedling again, and surviving rate can bring up to 92.8%.And simple to operate, with low cost, but has certain generalization.
Description of drawings
Fig. 1 is process flow sheet of the present invention.
Embodiment
In order to make technique means of the present invention, creation characteristic, workflow, using method reach purpose and effect is easy to understand, below further set forth the present invention.
The preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising, the required raw material of described selenium-rich nutritive agent consists of: selenium mineral powder 10kg, humic acids 25kg, potassium primary phosphate 10kg, urea 15kg, subtilis bean pulp fermentation liquid 40kg, subtilis bean pulp fermentation liquid is coexisted by solid-state and liquid, forms saturated solution after reaction.Preparation subtilis bean pulp fermentation liquid 50kg needs 15kg dregs of beans, 34kg water, 1L subtilis bacteria suspension; Concrete steps are: first with the actication of culture of subtilis, then dregs of beans is crushed to 600 orders and prepares bean pulp fermentation liquid, obtains subtilis bean pulp fermentation liquid in 72 hours at 37 ℃ of bottom fermentations; After subtilis bean pulp fermentation liquid is ready to, then be mixed in container with broken selenium mineral powder to 400 orders and activation, rotating speed 250r/min, 37 ℃ of lower constant temperature culture 36 hours; Then respectively potassium primary phosphate, urea are crushed to 400 orders below 70% at relative air humidity, prevent the suction moisture regain; Join again in said vesse, carry out the shaking table concussion, add at last humic acids to carry out stirring and evenly mixing, namely get the selenium-rich nutritive agent.
The using method of product selenium-rich nutritive agent: during hardening, with the concentration wiring solution-forming of selenium-rich nutritive agent with 300 times of dilutions, watered every 3 days.
Above demonstration and described ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that describes in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in protection claimed range of the present invention.Claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (8)
1. the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a raising, it is characterized in that: according to weight percent, the required raw material of described selenium-rich nutritive agent consists of: selenium mineral powder 5～10%, humic acids 20～25%, potassium primary phosphate 5～10%, urea 5～15%, subtilis bean pulp fermentation liquid 35～40%, and the weight percent sum of above raw material is 100%;
Described preparation method specifically comprises the following steps:
Preparation subtilis bean pulp fermentation liquid: 7～15kg dregs of beans, 18～35kg water, 0.5～1L subtilis bacteria suspension; Concrete steps are: with the actication of culture of subtilis and with the dregs of beans pulverizing, then with both mixed fermentation, namely obtain subtilis bean pulp fermentation liquid;
Selenium mineral powder is pulverized and is activated selenium mineral powder;
The selenium mineral powder that the subtilis bean pulp fermentation liquid of step (1) preparation and step (2) are activated is mixed in container to be cultivated;
Potassium primary phosphate, urea are pulverized, then joined in said vesse and concussion;
At last humic acids is added in said vesse and stirring and evenly mixing, namely get the selenium-rich nutritive agent.
2. the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising according to claim 1, it is characterized in that: the subtilis bean pulp fermentation liquid described in step (1) is coexisted by solid-state and liquid, forms saturated solution after reaction.
3. the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising according to claim 1, it is characterized in that: in step (1) according to weight ratio, dregs of beans: water: subtilis=15:34:1.
4. the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising according to claim 1, it is characterized in that: step is crushed to 600 orders with dregs of beans in (1), adds 1kg sucrose and 0.15kg potassium primary phosphate powder during preparation bean pulp fermentation liquid.
5. view and admire the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate according to a kind of raising claimed in claim 1, it is characterized in that: the fermentation condition in step (1) was: 37 ℃ of bottom fermentations 72 hours.
6. the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising according to claim 1, it is characterized in that: step is crushed to 400 orders with selenium mineral powder in (2).
7. the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising according to claim 1, it is characterized in that: the culture condition in step (3) was: rotating speed 250r/min, 37 ℃ of lower constant temperature culture 36 hours.
8. the preparation method of the selenium-rich nutritive agent of chrysanthemum tissue cultured seedling surviving rate is viewed and admired in a kind of raising according to claim 1, it is characterized in that: in step (4), potassium primary phosphate, urea are crushed to 400 orders below 70% at relative air humidity.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103755430A (en) * | 2013-09-24 | 2014-04-30 | 辽宁北纬有机肥科技有限公司 | Environmental protection type full-nutrition organic cultivation media and production method thereof |
| CN104557340A (en) * | 2015-02-09 | 2015-04-29 | 刘备 | Special microbial organic fertilizer for selenium-enriched rice |
| CN105037053A (en) * | 2015-09-08 | 2015-11-11 | 曾济天 | Selenium-enriched vitality nutrient and preparation method and application thereof |
| CN105481492A (en) * | 2014-09-16 | 2016-04-13 | 长沙学院 | Selenium-rich nutritional agent for improving biosynthesis of ramie wood fiber and preparation method of nutritional agent |
| CN105859356A (en) * | 2016-04-22 | 2016-08-17 | 长沙学院 | Special plant nutrient for producing polypeptide vegetables and preparation method thereof |
| CN105906389A (en) * | 2016-06-06 | 2016-08-31 | 战怡静 | Preparation method and product of efficient yield-increasing seaweed fertilizer |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
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| CN102320879A (en) * | 2011-09-06 | 2012-01-18 | 李子先 | High nutrient compound selenium fertilizer and preparation method thereof |
| CN102701804A (en) * | 2011-04-26 | 2012-10-03 | 长沙学院 | Paddy rice plant nutritional agent for producing high-essential amino acid rice |
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- 2013-03-15 CN CN201310083036.6A patent/CN103130549B/en not_active Expired - Fee Related
Patent Citations (4)
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| JPH0859379A (en) * | 1994-08-12 | 1996-03-05 | Bio Baransu:Kk | Septic treatment of animal excreta and its treated matter |
| CN1657509A (en) * | 2005-03-10 | 2005-08-24 | 浙江丰产农业科技有限公司 | Production method of selenium-enriched yield increasing agent |
| CN102701804A (en) * | 2011-04-26 | 2012-10-03 | 长沙学院 | Paddy rice plant nutritional agent for producing high-essential amino acid rice |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103755430A (en) * | 2013-09-24 | 2014-04-30 | 辽宁北纬有机肥科技有限公司 | Environmental protection type full-nutrition organic cultivation media and production method thereof |
| CN103755430B (en) * | 2013-09-24 | 2015-08-12 | 辽宁北纬有机肥科技有限公司铁岭县分公司 | A kind of production method of Environmental protection type full-nutrition organic cultivation media |
| CN105481492A (en) * | 2014-09-16 | 2016-04-13 | 长沙学院 | Selenium-rich nutritional agent for improving biosynthesis of ramie wood fiber and preparation method of nutritional agent |
| CN104557340A (en) * | 2015-02-09 | 2015-04-29 | 刘备 | Special microbial organic fertilizer for selenium-enriched rice |
| CN105037053A (en) * | 2015-09-08 | 2015-11-11 | 曾济天 | Selenium-enriched vitality nutrient and preparation method and application thereof |
| CN105859356A (en) * | 2016-04-22 | 2016-08-17 | 长沙学院 | Special plant nutrient for producing polypeptide vegetables and preparation method thereof |
| CN105906389A (en) * | 2016-06-06 | 2016-08-31 | 战怡静 | Preparation method and product of efficient yield-increasing seaweed fertilizer |
| CN106831149A (en) * | 2017-02-25 | 2017-06-13 | 湖南隆农农业科技开发有限公司 | A kind of micro-organism fertilizer for improving soil se use efficiency and preparation method thereof |
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