CN105859356A - Special plant nutrient for producing polypeptide vegetables and preparation method thereof - Google Patents
Special plant nutrient for producing polypeptide vegetables and preparation method thereof Download PDFInfo
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- CN105859356A CN105859356A CN201610257117.7A CN201610257117A CN105859356A CN 105859356 A CN105859356 A CN 105859356A CN 201610257117 A CN201610257117 A CN 201610257117A CN 105859356 A CN105859356 A CN 105859356A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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- Pest Control & Pesticides (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention provides a special plant nutrient for producing polypeptide vegetables. The special plant nutrient is prepared from the following raw materials in parts by mass: 15-20 parts of soybean meal, 25-30 parts of cottonseed meal, 15-30 parts of brewer distiller grains, 2-5 parts of molasses, 2-3 parts of urea, 6-10 parts of inorganic salt, 0.003-0.0005 part of sodium selenite, wherein the volume ratio of bacillus subtilis YS-45 seed bacteria suspension is 4-8%; the inorganic salt is prepared from a mixture of KH2PO4, MnSO4, ZnSO4 and borax according to a mass ratio of 2 to 1 to 6 to 3; the bacillus subtilis YS-45 is fermented, so that proteins in the materials such as cottonseed meal, soybean meal and the brewer distiller grains and the like can be decomposed to generate a plurality of soluble polypeptides; the fermented product is applied to the vegetables; the polypeptide materials in the nutrient solution can be absorbed and stored by the vegetables, so that the content of polypeptides in the vegetables can be increased.
Description
Technical field
The present invention relates to plant nutrient agent, be particularly used for produce polypeptide vegetables tailored version plant nutrient agent and
Its preparation method, belongs to agriculture field.
Background technology
Society, the nutrition and health care consciousness of people is increasingly stronger, and vegetables are the necessitys of our daily life,
Enough vegetables intakes are to maintain healthy basic.Polypeptide has effect of health care, can control growth,
Growth, immunological regulation and metabolism, mend peptide and mend activity exactly, mends peptide and mends vigor exactly, mends peptide and mends exactly
Life.In common vegetable, content of peptides is the lowest, in order to allow the nutritional labeling of vegetables is more complete, quality is higher,
More healthy, produce a kind of polypeptide vegetables tailored version nutritional agents that can increase vegetables content of peptides and just seem the heaviest
Want.
Summary of the invention
Aim to provide a kind of polypeptide vegetables tailored version plant battalion that can increase vegetables content of peptides the mesh of the present invention
Support agent.
It is to provide the preparation method for producing polypeptide vegetables tailored version plant nutrient agent another mesh of the present invention.
Technical scheme is as follows:
For producing polypeptide vegetables tailored version plant nutrient agent, raw material and mass fraction thereof be: dregs of beans: 15~
20 parts, cotton dregs: 25~30 parts, brewex's grains: 15~30 parts, molasses: 2~5 parts, urea: 2~3 parts,
Inorganic salts: 6~10 parts, sodium selenite: 0.003~0.005 part;Bacillus subtilis YS-45 kind daughter bacteria
The volume accounting of suspension is 4%~8%, and wherein, inorganic salts are KH by mass ratio2PO4:MnSO4:ZnSO4: boron
The mixture composition of sand=1~3:0.5~1.5:4~8:2~4, it is preferred that each composition quality of described inorganic salts
Ratio is KH2PO4:MnSO4:ZnSO4: borax=2:1:6:3.
For producing the preparation method of polypeptide vegetables tailored version plant nutrient agent, comprise the following steps:
A, the preparation of bacillus subtilis YS-45 seed bacteria suspension:
A-1, the preparation of murphy juice: in mass ratio for potato: the ratio of water=1:4~6 prepares murphy juice,
After potato is added water, boil 25~35 minutes, after filter and remove residue, obtain murphy juice;
A-2, the preparation of seed culture medium: according to for producing polypeptide vegetables tailored version plant nutrient agent
The mass fraction of each raw material weighs other raw materials in addition to bacillus subtilis YS-45 seed bacteria suspension,
The dregs of beans weighed, cotton dregs, brewex's grains, molasses, urea, inorganic salts, sodium selenite are added seed
In culture vessel, add murphy juice to seed culture vessel volume 25~35%, 110~130 DEG C,
Seed culture medium is obtained after sterilizing in 25~35 minutes;
A-3, actication of culture and cultivation: an activated spawn adds 9~11ml sterilized waters and makes bacteria suspension,
Again bacteria suspension is inoculated in seed culture medium by the volume ratio of 4%~8%, then at 250r/min,
Bacillus subtilis YS-45 seed bacteria suspension is i.e. obtained after cultivating 23~25 hours under the conditions of 35~39 DEG C;
B, weigh except withered grass according to the mass fraction for the raw material that produces polypeptide vegetables tailored version plant nutrient agent
Other raw materials beyond bacillus YS-45 seed bacteria suspension, by the dregs of beans weighed, cotton dregs, brewex's grains,
Molasses, urea, inorganic salts, sodium selenite add in fermentation tank, then add water to fermenter volume 65~
75%, stirring mixing, sterilizing 25~35 minutes at 110~130 DEG C, add by the volume ratio of 4%~8% after cooling
Enter the bacillus subtilis YS-45 seed bacteria suspension prepared, 250r/min, 35~39 DEG C, PH from
Semi-finished product I are obtained after fermenting 46~50 hours under conditions of Ran;
C, above-mentioned semi-finished product I are filtered, add palm to the ratio that filtrate by volume percentage is 1%~2%
Oil, obtains for producing polypeptide vegetables tailored version plant nutrient agent.
Wherein, when preparing bacillus subtilis YS-45 seed bacteria suspension, before prepared by seed culture medium
Within one day, carry out bacillus subtilis YS-45 actication of culture.
Preferably, in mass ratio for potato time prepared by murphy juice: the ratio of water=1:5 prepares murphy juice, by soil
After beans add water, boil 30 minutes, after filter and remove residue, obtain murphy juice.
Preferably, after the raw material weighed being added seed culture vessel time prepared by seed culture medium, soil is added
Fermented bean drink to the 30% of seed culture vessel volume, 121 DEG C, after sterilizing in 30 minutes and get final product.
Preferably, an activated spawn adds 10ml sterilized water and makes bacteria suspension, then is pressed by described bacteria suspension
The volume ratio of 4%~8% is inoculated in seed culture medium, then at 250r/min, cultivates 24 under the conditions of 37 DEG C
Bacillus subtilis YS-45 seed bacteria suspension is i.e. obtained after hour.
Preferably, in step B, according to the matter for producing the polypeptide vegetables each raw material of tailored version plant nutrient agent
Amount number weigh other raw materials in addition to bacillus subtilis YS-45 seed bacteria suspension, by the dregs of beans weighed,
Cotton dregs, brewex's grains, molasses, urea, inorganic salts, sodium selenite add in fermentation tank, then add water to send out
The 70% of ferment tank volume, stirring mixing, sterilizing 30 minutes at 121 DEG C, press the volume ratio of 4%~8% after cooling
Add bacillus subtilis YS-45 seed bacteria suspension, 250r/min, 37 DEG C, PH naturally under the conditions of
Semi-finished product I are obtained after fermenting 48 hours.
The know-why of the present invention is: bacillus subtilis YS-45 fermentation makes cotton dregs, dregs of beans, brewex's grains etc.
Breaks down proteins in material, generates multiple soluble polypeptide, is sprayed by tunning on vegetables, nutrition
Peptide material in liquid is absorbed by vegetables and stores, thus improves the content of peptides in vegetables, simultaneously because inhale
Receive sodium selenite, added the content of vegetables Organic Selenium, be finally translated into and human body is had health-care efficacy
Organic Selenium, after the vegetables that this mode plants out are eaten by human body, the material such as polypeptide therein, Organic Selenium
It is absorbed by the body.
Advantages of the present invention and beneficial effect: the present invention can increase the content of peptides in vegetables, can be by human body
Directly absorb, improve the immunity of human body, regulation metabolism, promote the formation of new cell, replace aging
Cell perform physiological function play the effect delayed senility, add the ore deposits such as vegetables content of peptides, Organic Selenium
Content of material, has health-care effect to human body.
Detailed description of the invention
Below in conjunction with specific embodiment, be used for the present invention producing polypeptide vegetables tailored version plant nutrient agent and
Its preparation method is described in further detail.
Embodiment 1:
First prepare bacillus subtilis YS-45 seed bacteria suspension: weighing peeled potatoes 48g, add water 240ml,
Boil 30 minutes, filter and remove residue, then constant volume is to 240ml;Raw material is weighed according to the mass fraction of described raw material,
Weigh dregs of beans 15g, cotton dregs 25g, brewex's grains 15g, molasses 2g, urea 2g, inorganic salts (KH2PO4 1g、
MnSO4 0.5g、ZnSO43g, borax 1.5g), sodium selenite 0.003g, by these materials add 1000ml
In conical flask, then in conical flask add murphy juice to 300ml, 121 DEG C, sterilizing in 30 minutes cooling after access before
The bacillus subtilis YS-45 bacteria suspension 12ml (by 4% calculating of culture volume 300ml) of activation in a day,
250r/min, 37 DEG C of Shaking culture i.e. obtain bacillus subtilis YS-45 seed bacteria suspension after 24 hours.
Weigh raw material according still further to the mass fraction of described raw material: dregs of beans 150g, cotton dregs 250g, brewex's grains 150g,
Molasses 20g, urea 20g, inorganic salts (KH2PO4 10g、MnSO4 5g、ZnSO430g, borax 15g),
Sodium selenite 0.03g, adds these materials in 10L fermentation tank, adds water to about 7L, mix latter 121 DEG C,
Sterilizing 30min, accesses bacillus subtilis YS-45 seed bacteria suspension 280ml (4% calculating of 7L volume) after cooling,
250r/min, 37 DEG C, PH spontaneous fermentation 48h.After fermenting 48 hours, filter, filtrate is added 70ml
Palm oil (1% calculating of 7L volume), is for producing polypeptide vegetables tailored version plant nutrient agent.This is many
Peptide vegetables tailored version plant nutrient agent foliage-spray is on vegetables (as a example by Chinese cabbage), and spray is 50ml/ in right amount
Pluck in Chinese cabbage and to start to spray for first 1 month, spray after 5 at dusk and be advisable, spray in every 3 days once, even spray 3
Secondary, concentration is 8%, sprays and occurs that mist pearl does not fall down with blade face blade back and be advisable.
Embodiment 2:
First prepare bacillus subtilis YS-45 seed bacteria suspension: weighing peeled potatoes 48g, add water 240ml,
Boil 30 minutes, filter and remove residue, then constant volume is to 240ml;Raw material is weighed by the mass fraction of described raw material,
By the dregs of beans 18g weighed, cotton dregs 28g, brewex's grains 25g, molasses 4g, urea 3g, inorganic salts (KH2PO4
1.5g、MnSO4 0.75g、ZnSO42.25g, borax 4.5g), sodium selenite 0.005g, by these things
Matter adds in 1000ml conical flask, then adds murphy juice in conical flask to 300ml, 121 DEG C, 30 minutes
The bacillus subtilis YS-45 bacteria suspension 18ml of activation the previous day is accessed (by culture volume after sterilizing cooling
6% calculating of 300ml), 250r/min, 37 DEG C of Shaking culture 24 hours.Obtain bacillus subtilis YS-45
Seed bacteria suspension.
Weigh raw material according still further to the mass fraction of described raw material: dregs of beans 200g, cotton dregs 280g, brewex's grains 200g,
Molasses 40g, urea 20g, inorganic salts (KH2PO415g、MnSO47.5g、ZnSO422.5g, borax 45g),
Sodium selenite 0.05g, adds these materials in 10L fermentation tank, adds water to about 7L, mix latter 121 DEG C,
Sterilizing 30 minutes, accesses bacillus subtilis YS-45 seed bacteria suspension 350ml (by 7L volume after cooling
5% calculating), 250r/min, 37 DEG C, PH spontaneous fermentation 48 hours.After fermenting 48 hours, filter,
In filtrate, add 70ml palm oil, be for producing polypeptide vegetables tailored version plant nutrient agent.This is many
Peptide vegetables tailored version plant nutrient agent foliage-spray is on vegetables (as a example by celery), and spray is 1L/ square meter in right amount,
Once, even spray 3 times, concentration is 10%, sprays and occurs that mist pearl does not falls down with blade face blade back in spray in every 5 days
It is advisable.
Embodiment 3:
First prepare bacillus subtilis YS-45 seed bacteria suspension: weighing peeled potatoes 50g, add water 250ml,
Boil 30 minutes, filter and remove residue, then constant volume is to 250ml;Raw material is weighed according to the mass fraction of described raw material,
By the dregs of beans 20g weighed, cotton dregs 25g, brewex's grains 20g, molasses 2g, urea 2g, inorganic salts (KH2PO4
1g、MnSO4 0.5g、ZnSO43g, borax 1.5g), sodium selenite 0.004g, these materials are added
In 1000ml conical flask, then in conical flask add murphy juice to 300ml, 121 DEG C, sterilizing in 30 minutes cold
Access the bacillus subtilis YS-45 bacteria suspension 24ml of activation the previous day the most afterwards (by culture volume 300ml
8% calculating), 250r/min, 37 DEG C of Shaking culture i.e. obtain bacillus subtilis YS-45 seed after 24 hours
Bacteria suspension.
Weigh raw material according still further to the mass fraction of described raw material: dregs of beans 200g, cotton dregs 280g, brewex's grains 200g,
Molasses 40g, urea 20g, inorganic salts (KH2PO4 15g、MnSO4 7.5g、ZnSO422.5g, borax
45g), sodium selenite 0.05g, these materials are added in 10L fermentation tank, add water to about 7L, mixing
Latter 121 DEG C, sterilizing minute, access bacillus subtilis YS-45 seed bacteria suspension 560ml and (press after cooling
8% calculating of 7L volume), 250r/min, 37 DEG C, PH spontaneous fermentation 48 hours.After fermenting 48 hours,
Filter, filtrate is added 70ml palm oil, is for producing polypeptide vegetables tailored version plant nutrient agent.
By this polypeptide vegetables tailored version plant nutrient agent foliage-spray on vegetables (as a example by tomato), spray be in right amount
200ml/ strain, tomato sprays 1 time florescence, after blossom fall one week, within each 7 days, sprays 1 time, even spray
2 times, concentration is 10%, sprays and occurs that mist pearl does not fall down with blade face blade back and be advisable.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Any amendment, equivalent and the improvement etc. made within bright spirit and principle, should be included in the present invention
Protection domain within.
Claims (9)
1. it is used for producing polypeptide vegetables tailored version plant nutrient agent, it is characterised in that raw material and mass fraction thereof be:
Dregs of beans: 15~20 parts, cotton dregs: 25~30 parts, brewex's grains: 15~30 parts, molasses: 2~5 parts, urine
Element: 2~3 parts, inorganic salts: 6~10 parts, sodium selenite: 0.003~0.005 part;Bacillus subtilis
The volume accounting of YS-45 seed bacteria suspension is 4%~8%.
It is used for the most as claimed in claim 1 producing polypeptide vegetables tailored version plant nutrient agent, it is characterised in that institute
Stating inorganic salts by mass ratio is KH2PO4:MnSO4:ZnSO4: borax=1~3:0.5~1.5:4~8:2~4
Mixture forms.
It is used for the most as claimed in claim 2 producing polypeptide vegetables tailored version plant nutrient agent, it is characterised in that institute
State each composition quality of inorganic salts than for KH2PO4:MnSO4:ZnSO4: borax=2:1:6:3.
4. the preparation for producing polypeptide vegetables tailored version plant nutrient agent as described in any one of claims 1 to 3
Method, it is characterised in that comprise the following steps:
A, the preparation of bacillus subtilis YS-45 seed bacteria suspension:
A-1, the preparation of murphy juice: in mass ratio for potato: the ratio of water=1:4~6 prepares murphy juice,
After potato is added water, boil 25~35 minutes, after filter and remove residue, obtain murphy juice;
A-2, the preparation of seed culture medium: claim according to the mass fraction of each raw material described in claim 1
Remove other raw materials beyond bacillus subtilis YS-45 seed bacteria suspension, by the dregs of beans weighed, cotton
The dregs of rice, brewex's grains, molasses, urea, inorganic salts, sodium selenite add in seed culture vessel, then add
Enter murphy juice described in step A-1 to seed culture vessel volume 25~35%, 110~130 DEG C,
Seed culture medium is obtained after sterilizing in 25~35 minutes;
A-3, actication of culture and cultivation: an activated spawn adds 9~11ml sterilized waters and makes bacteria suspension,
Again by described bacteria suspension as 4%~8% volume ratio be inoculated in the seed culture medium described in step A-2,
Then at 250r/min, after cultivating 23~25 hours under the conditions of 35~39 DEG C, bacillus subtilis is i.e. obtained
YS-45 seed bacteria suspension;
B, weigh except bacillus subtilis YS-45 kind according to the mass fraction of each raw material described in claim 1
Other raw materials beyond daughter bacteria suspension, by the dregs of beans weighed, cotton dregs, brewex's grains, molasses, urea, inorganic
Salt, sodium selenite add in fermentation tank, then add water to the 65~75% of fermenter volume, and stirring mixes,
Sterilizing 25~35 minutes at 110~130 DEG C, after cooling, the volume ratio as 4%~8% adds described in step A
Bacillus subtilis YS-45 seed bacteria suspension, 250r/min, 35~39 DEG C, PH naturally under the conditions of
Semi-finished product I are obtained after fermenting 46~50 hours;
C, the semi-finished product I described in step B are filtered, to the ratio that filtrate by volume percentage is 1%~2%
Add palm oil, obtain for producing polypeptide vegetables tailored version plant nutrient agent.
5. the preparation method for producing polypeptide vegetables tailored version plant nutrient agent as claimed in claim 4, it is special
Levy and be, prepare bacillus subtilis YS-45 seed bacteria suspension time, seed culture medium prepare previous
It carries out bacillus subtilis YS-45 actication of culture.
6. the preparation method for producing polypeptide vegetables tailored version plant nutrient agent as claimed in claim 4, it is special
Levy and be, in described step A-1, in mass ratio for potato time prepared by murphy juice: prepared by the ratio of water=1:5
Murphy juice, after potato is added water, boils 30 minutes, obtains murphy juice after filter and remove residue.
7. the preparation method for producing polypeptide vegetables tailored version plant nutrient agent as claimed in claim 4, it is special
Levy and be, in described step A-2, time prepared by seed culture medium, the raw material weighed is added seed culture vessel
After, add murphy juice to the 30% of seed culture vessel volume, 121 DEG C, after sterilizing in 30 minutes seed training
Support base.
8. the preparation method for producing polypeptide vegetables tailored version plant nutrient agent as claimed in claim 4, it is special
Levying and be, in described step A-3, an activated spawn adds 10ml sterilized water and makes bacteria suspension, then by institute
State bacteria suspension to be inoculated in the seed culture medium of described step A-2 by the volume ratio of 4%~8%, then exist
250r/min, i.e. obtains bacillus subtilis YS-45 seed bacteria suspension after cultivating 24 hours under the conditions of 37 DEG C.
9. the preparation method for producing polypeptide vegetables tailored version plant nutrient agent as claimed in claim 4, it is special
Levy and be, in described step B, weigh except withered grass according to the mass fraction of each raw material described in claim 1
Other raw materials beyond bacillus YS-45 seed bacteria suspension, by the dregs of beans weighed, cotton dregs, brewex's grains,
Molasses, urea, inorganic salts, sodium selenite add in fermentation tank, then add water to the 70% of fermenter volume,
Stirring mixing, sterilizing 30 minutes at 121 DEG C, after cooling, the volume ratio as 4%~8% adds prepared by step A
Bacillus subtilis YS-45 seed bacteria suspension, 250r/min, 37 DEG C, the natural condition of PH issues
Ferment obtains semi-finished product I after 48 hours.
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CN106431577A (en) * | 2016-10-14 | 2017-02-22 | 中国科学院沈阳应用生态研究所 | Selenium-rich double-effect water-soluble fertilizer and preparation method thereof |
CN108840727A (en) * | 2018-07-19 | 2018-11-20 | 长沙学院 | A kind of plant nutrient agent and preparation method thereof producing high-quality balsam pear |
CN109438006A (en) * | 2018-11-19 | 2019-03-08 | 南京工业大学 | Preparation method of aeroponic green agricultural biological product nutrient solution |
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CN104692957A (en) * | 2015-03-03 | 2015-06-10 | 安徽远大机械制造有限公司 | Biological organic fertilizer containing bacillus subtilis and application thereof |
CN105272412A (en) * | 2015-10-23 | 2016-01-27 | 广西锦翰环保科技有限公司 | Organic-Se nutrient solution for crops as well as preparation method and application of organic-Se nutrient solution |
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CN106431577A (en) * | 2016-10-14 | 2017-02-22 | 中国科学院沈阳应用生态研究所 | Selenium-rich double-effect water-soluble fertilizer and preparation method thereof |
CN108840727A (en) * | 2018-07-19 | 2018-11-20 | 长沙学院 | A kind of plant nutrient agent and preparation method thereof producing high-quality balsam pear |
CN109438006A (en) * | 2018-11-19 | 2019-03-08 | 南京工业大学 | Preparation method of aeroponic green agricultural biological product nutrient solution |
CN109438006B (en) * | 2018-11-19 | 2021-12-28 | 南京工业大学 | Preparation method of aeroponic green agricultural biological product nutrient solution |
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