CN103342593A - Organic selenium crop nutrient and preparation method for same - Google Patents
Organic selenium crop nutrient and preparation method for same Download PDFInfo
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Abstract
The invention discloses an organic selenium crop nutrient and a preparation method for the same. The organic selenium crop nutrient comprises the following raw materials in parts by weight: 0.5-1.5 parts of molasses, 5-15 parts of soybean meal amino acid, 6-9 parts of urea, and 0.04-0.1 part of sodium selenite. The preparation method for the organic selenium crop nutrient comprises the following steps of: oscillating all raw materials for 24-72 hours in the conditions of a rotation speed of 120-200 rpm and a temperature of 35-40 DEG C, and sieving to obtain a filtrate, namely, the organic selenium crop nutrient. The content of organic selenium in the organic selenium crop nutrient disclosed by the invention is greater than 0.3 g/L. Additionally, according to the organic selenium crop nutrient and the preparation method disclosed by the invention, harm brought by the too high dosage of an inorganic selenium fertilizer to a human body, and fertilizer damage phenomena of seedling burning, leaf spots and the like due to the inappropriate dosage of the inorganic selenium fertilizer are overcome.
Description
Technical field
The present invention relates to a kind of crop alimentary agent, be specially the plant nutrient agent that contains organoselenium, comprise that selenomethionine adds the organoselenium plant nutrient agent of plant nutrient agent and fermentative production.
Background technology
Because input and the use of a large amount of chemical fertilizer, the variation of some physico-chemical properties appears in the soil, bring many negative impacts to agricultural, therefore, many scholars biological organic fertilizer that begins one's study is realized the benign cycle utilization in high yield and soil, wherein the blade face fertilizer is present reasonable one big class fertilizer, at present more than 2,000 organic water-soluble foliage fertilizers such as existing humic-acid kind, amino acids, marine alga acids.In China, most people seriously lacks trace element-selenium, and the nutritionist points out that China area over half lacks selenium.In order to mend selenium, farm crop mainly are the foliage fertilizers that contains Sodium Selenite by spraying at present, to increase selenium constituent content in the agricultural-food.In fodder industry, main by adding Sodium Selenite, increase the selenium constituent content in the meat.But the inorganic selenium especially excessive meeting of Sodium Selenite is detrimental to health, and selenium mainly is to rely on organoselenium and work to health care, the protective effect on cancer risk of human body.Country is to inorganic selenium content finite quantity standard in the agricultural-food, can not surpass 0.3 mg/kg as the cereals selenium content, and organoselenium (refer to the selenium element replace element sulphur in the organism and generate contain the selenium organism, as selenomethionine, seleno-cysteine, selenocystine etc.) then do not have a limit standard.Have not yet to see the fertilizer, nutrition agent, the fodder additives that contain organoselenium, be necessary to develop the crop alimentary agent that contains organoselenium for this reason, and can be used as the organoselenium fodder additives.
Summary of the invention
The object of the present invention is to provide a kind of organoselenium crop alimentary agent and preparation method thereof, this crop alimentary agent is to be raw material with the dregs of beans, add selenium and other trace elements, after subtilis YS-45 fermentation, adding an amount of macronutrient configuration forms, be rich in the liquid preparation of indispensable amino acid and organism organic selenium, application on fruits and vegetables and paddy rice shows, can significantly improve crop output, improve product quality, especially improve the organic selenium content of crop, seem very necessary for the raising of crop organic selenium content.
For achieving the above object, the invention provides following technical scheme:
The agent of a kind of organoselenium crop alimentary comprises according to the raw material of weight part: 0.5～1.5 part in molasses, 5～15 parts in dregs of beans amino acid, 6～9 parts in urea, 0.04～0.1 part of Sodium Selenite.
As further scheme: the agent of described organoselenium crop alimentary comprises according to the raw material of weight part: 1 part in molasses, 0 part of dregs of beans amino acid/11,7.5 parts in urea, 0.07 part of Sodium Selenite.
The preparation method of described organoselenium crop alimentary agent is that 120～200rpm, temperature are the 24～72h that vibrates under 35～40 ℃ the condition with all raw materials at rotating speed, sieve filtered liquid, be the agent of organoselenium crop alimentary.
As further scheme: the preparation method of described organoselenium crop alimentary agent is that 160rpm, temperature are the 48h that vibrates under 37 ℃ the condition with all raw materials at rotating speed.
As further scheme: described sieving is to cross 60 mesh sieves.
As further scheme: the amino acid whose production method of described dregs of beans: adopt the fermentation of bacillus subtilis dregs of beans of giving birth in the citrus to produce dregs of beans amino acid; Concrete steps are as follows:
(1) cultivation of kind daughter bacteria:
1) 15%～25% murphy juice is made: per 150～250g potato adds water 1000ml, boils 25～35min, and 50～70 mesh sieves filter, filtrate for later use;
2) making of seed bacterium culture medium: the preparation of raw material according to following weight parts becomes seed culture medium, and raw material is: 4～8 parts of bean cake powders, 0.2～0.4 part in molasses, KH
2PO
490～93 parts of 1～3 parts, murphy juice; Described bean cake powder is crossed 50～70 mesh sieves; The sterilising conditions of seed culture medium is: 110～130 ℃, and 28～35min;
3) plant the daughter bacteria actication of culture: in preceding 12～48 hours of kind of daughter bacteria cultivation, carry out the Bacillus subtilis strain activation of giving birth in the citrus; The amount of activated spawn switching slant tube press 4%～6% of seed culture medium volume and is calculated, and 1 activated spawn adds 8～12ml sterilized water and makes bacteria suspension, is inoculated into the bacteria suspension of careless genus bacillus living in the citrus that seed culture medium obtains activating; The condition of actication of culture is: 25～35 ℃, cultivate 12～36h;
4) inoculation: press the bacteria suspension that 4%～6% of seed culture medium volume inserts the careless genus bacillus that gives birth in the activated citrus, shake up;
5) shake-flask culture: be that 120～200rpm shakes in the bottle at rotating speed, cultivate 12～36h down for 30～40 ℃ and obtain kind of a daughter bacteria;
(2) ferment tank:
1) making of fermentation substrate: the preparation of raw material according to following weight parts becomes fermentation substrate, and raw material is: 4～8 parts of bean cake powders, 0.2～0.4 part in molasses, KH
2PO
490～93 parts of 1～3 parts, murphy juice; Described bean cake powder is crossed 50～70 mesh sieves;
2) fermentation substrate sterilising conditions: 110～130 ℃, 28～35min, the cooling back is standby;
3) inoculation kind of daughter bacteria: 8%～12% inoculation kind of the daughter bacteria of pressing the fermentation substrate volume;
4) fermentation condition: 200～300rpm, 30～45 ℃, fermentation time 48～96h;
5) filtering fermentation liquor: after fermentation is finished, fermented liquid is filtered with 80～120 mesh sieves, namely obtain the dregs of beans amino acid solution.
As further scheme again: shaking culture is adopted in the cultivation of planting daughter bacteria, and concrete steps are as follows:
1) 20% murphy juice is made: every 200g potato adds water 1000ml, boils 30min, and 60 mesh sieves filter, filtrate for later use;
2) making of seed bacterium culture medium: the preparation of raw material according to following weight parts becomes seed culture medium, and raw material is: 6 parts of bean cake powders, 0.3 part in molasses, KH
2PO
491.7 parts of 2 parts, murphy juice; Described bean cake powder is crossed 60 mesh sieves; The sterilising conditions of seed culture medium is: 121 ℃, and 30min;
3) plant the daughter bacteria actication of culture: in preceding 24 hours of kind of daughter bacteria cultivation, carry out the Bacillus subtilis strain activation of giving birth in the citrus; The amount of activated spawn switching slant tube press 5% of seed culture medium volume and is calculated, and 1 activated spawn adds the 10ml sterilized water and makes bacteria suspension, is inoculated into the bacteria suspension of careless genus bacillus living in the citrus that seed culture medium obtains activating; The condition of actication of culture is: 30 ℃, cultivate 24h;
4) inoculation: press the bacteria suspension that 5% of seed culture medium volume inserts the careless genus bacillus that gives birth in the activated citrus, shake up;
5) shake-flask culture: be that 160rpm shakes in the bottle at rotating speed, cultivate 24h down for 37 ℃ and obtain kind of a daughter bacteria.
As further scheme again: ferment tank, concrete steps are as follows:
1) making of fermentation substrate: the preparation of raw material according to following weight parts becomes fermentation substrate, and raw material is: 6 parts of bean cake powders, 0.3 part in molasses, KH
2PO
491.7 parts of 2 parts, murphy juice; Described bean cake powder is crossed 60 mesh sieves;
2) fermentation substrate sterilising conditions: 121 ℃, 30min, the cooling back is standby;
3) inoculation kind of daughter bacteria: 10% inoculation kind of the daughter bacteria of pressing the fermentation substrate volume;
4) fermentation condition: 250rpm, 37 ℃, fermentation time 72h;
5) filtering fermentation liquor: after fermentation is finished, fermented liquid is filtered with 100 mesh sieves, namely obtain the dregs of beans amino acid solution.
As scheme further again: described subtilis is subtilis YS-45.
Compared with prior art, the invention has the beneficial effects as follows: organic selenium content is more than 0.3 g/L in the organoselenium crop alimentary agent of the present invention's preparation; Organoselenium not only has no side effect to the people, and the more important thing is has health-care effect to the people, and therefore, organoselenium is described as " life kindling material ", " longevity factor ", " anticancer king "; This organoselenium crop alimentary agent has not only overcome the harm to human body that inorganic selenium fertilizer brings because consumption is too high; Fertilizer damage phenomenons such as the burning seedling that inorganic selenium fertilizer causes because consumption is improper, tikka have also been overcome.
Embodiment
Below in conjunction with the embodiment of the invention, the technical scheme in the embodiment of the invention is clearly and completely described, obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on the embodiment among the present invention, those of ordinary skills belong to the scope of protection of the invention not making the every other embodiment that obtains under the creative work prerequisite.
Embodiment 1
In the embodiment of the invention, according to the making processes of the raw material of weight part: 6% bean cake powder (60 sieve mesh) adds 2% molasses, 0.3% KH
2PO
4, 91.7% murphy juice, be mixed with seed culture medium, insert sward genus bacillus YS-45 bacteria suspension in the activated citrus in 5% ratio, 160rpm, 37 ℃, cultivate 24h, plant daughter bacteria.Then by 6% bean cake powder (60 sieve mesh), 2% molasses, 0.3% KH
2PO
4, 91.7% water mixed is even, inserts above-mentioned kind of daughter bacteria, 250rpm, 37 ℃, fermentation time 72h filters fermented liquid with industrial filter cloth, namely obtains the dregs of beans amino acid solution.Then, be the ratio of 1:10:7.5:0.07 in the mixed ratio of molasses, dregs of beans amino acid, urea, Sodium Selenite, 160rpm, 37 ℃, vibration 48h reaction obtains the agent of organoselenium crop alimentary.When paddy rice is used, dilute 25 times, in boot stage, heading stage, the full heading time foliar spray of paddy rice 3 times, consumption: 50 kilograms/mu/times, or the rice seedling bed whole good after, the top layer is shallow executes 20 kilograms/mu, test-results shows, makes the rice organic selenium content improve 23-49% after the agent of use organoselenium crop alimentary.
Embodiment 2
In the embodiment of the invention, according to the making processes of the raw material of weight part: 6% bean cake powder (60 sieve mesh), 2% molasses, 0.3% KH
2PO
4, 91.7% murphy juice, be mixed with seed culture medium, insert sward genus bacillus YS-45 bacteria suspension in the activated citrus in 5% ratio, 160rpm, 37 ℃, cultivate 24h, plant daughter bacteria.Then by 6% bean cake powder (60 sieve mesh), 2% molasses, 0.3% KH
2PO
4, 91.7% water mixed is even, inserts above-mentioned kind of daughter bacteria, 250rpm, 37 ℃, fermentation time 72h filters fermented liquid with industrial filter cloth, namely obtains the dregs of beans amino acid solution.Then, be the ratio of 1:10:7.5:0.07 in the mixed ratio of molasses, dregs of beans amino acid, urea, Sodium Selenite, 160rpm, 37 ℃, vibration 48h reaction obtains the agent of organoselenium crop alimentary.Mainly contain dual mode in the use on the cowpea: 1, pouring: carrying out soil pouring once in sowing with before blooming respectively, totally twice, consumption: 20 kilograms/mu; 2, spray: in cowpea flowering period, sprayed once every 7 days, totally three times, consumption: 50 kilograms/mu/times, test-results shows, makes the rice organic selenium content improve 23～49% after using the agent of organoselenium crop alimentary.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and under the situation that does not deviate from spirit of the present invention or essential characteristic, can realize the present invention with other specific form.Therefore, no matter from which point, all should regard embodiment as exemplary, and be nonrestrictive, scope of the present invention is limited by claims rather than above-mentioned explanation, therefore is intended to include in the present invention dropping on the implication that is equal to important document of claim and all changes in the scope.
In addition, be to be understood that, though this specification sheets is described according to embodiment, but be not that each embodiment only comprises an independently technical scheme, this narrating mode of specification sheets only is for clarity sake, those skilled in the art should make specification sheets as a whole, and the technical scheme among each embodiment also can form other embodiments that it will be appreciated by those skilled in the art that through appropriate combination.
Claims (9)
1. organoselenium crop alimentary agent is characterized in that, comprises according to the raw material of weight part: 0.5～1.5 part in molasses, 5～15 parts in dregs of beans amino acid, 6～9 parts in urea, 0.04～0.1 part of Sodium Selenite.
2. organoselenium crop alimentary according to claim 1 agent is characterized in that, comprises according to the raw material of weight part: 1 part in molasses, 0 part of dregs of beans amino acid/11,7.5 parts in urea, 0.07 part of Sodium Selenite.
3. preparation method as the agent of the arbitrary described organoselenium crop alimentary of claim 1～2, it is characterized in that, be that 120～200rpm, temperature are the 24～72h that vibrates under 35～40 ℃ the condition with all raw materials at rotating speed, sieve filtered liquid, be the agent of organoselenium crop alimentary.
4. the preparation method of organoselenium crop alimentary according to claim 3 agent is characterized in that, is that 160rpm, temperature are the 48h that vibrates under 37 ℃ the condition with all raw materials at rotating speed.
5. the preparation method of organoselenium crop alimentary according to claim 3 agent is characterized in that, described sieving to crossing 60 mesh sieves.
6. the preparation method of organoselenium crop alimentary according to claim 3 agent is characterized in that, the amino acid whose production method of described dregs of beans: adopt the fermentation of bacillus subtilis dregs of beans of giving birth in the citrus to produce dregs of beans amino acid; Concrete steps are as follows:
(1) cultivation of kind daughter bacteria:
1) 15%～25% murphy juice is made: per 150～250g potato adds water 1000ml, boils 25～35min, and 50～70 mesh sieves filter, filtrate for later use;
2) making of seed bacterium culture medium: the preparation of raw material according to following weight parts becomes seed culture medium, and raw material is: 4～8 parts of bean cake powders, 0.2～0.4 part in molasses, KH
2PO
490～93 parts of 1～3 parts, murphy juice; Described bean cake powder is crossed 50～70 mesh sieves; The sterilising conditions of seed culture medium is: 110～130 ℃, and 28～35min;
3) plant the daughter bacteria actication of culture: in preceding 12～48 hours of kind of daughter bacteria cultivation, carry out the Bacillus subtilis strain activation of giving birth in the citrus; The amount of activated spawn switching slant tube press 4%～6% of seed culture medium volume and is calculated, and 1 activated spawn adds 8～12ml sterilized water and makes bacteria suspension, is inoculated into the bacteria suspension of careless genus bacillus living in the citrus that seed culture medium obtains activating; The condition of actication of culture is: 25～35 ℃, cultivate 12～36h;
4) inoculation: press the bacteria suspension that 4%～6% of seed culture medium volume inserts the careless genus bacillus that gives birth in the activated citrus, shake up;
5) shake-flask culture: be that 120～200rpm shakes in the bottle at rotating speed, cultivate 12～36h down for 30～40 ℃ and obtain kind of a daughter bacteria;
(2) ferment tank:
1) making of fermentation substrate: the preparation of raw material according to following weight parts becomes fermentation substrate, and raw material is: 4～8 parts of bean cake powders, 0.2～0.4 part in molasses, KH
2PO
490～93 parts of 1～3 parts, murphy juice; Described bean cake powder is crossed 50～70 mesh sieves;
2) fermentation substrate sterilising conditions: 110～130 ℃, 28～35min, the cooling back is standby;
3) inoculation kind of daughter bacteria: 8%～12% inoculation kind of the daughter bacteria of pressing the fermentation substrate volume;
4) fermentation condition: 200～300rpm, 30～45 ℃, fermentation time 48～96h;
5) filtering fermentation liquor: after fermentation is finished, fermented liquid is filtered with 80～120 mesh sieves, namely obtain the dregs of beans amino acid solution.
7. the preparation method of organoselenium crop alimentary according to claim 6 agent is characterized in that, plants the cultivation of daughter bacteria and adopts shaking culture, and concrete steps are as follows:
1) 20% murphy juice is made: every 200g potato adds water 1000ml, boils 30min, and 60 mesh sieves filter, filtrate for later use;
2) making of seed bacterium culture medium: the preparation of raw material according to following weight parts becomes seed culture medium, and raw material is: 6 parts of bean cake powders, 0.3 part in molasses, KH
2PO
491.7 parts of 2 parts, murphy juice; Described bean cake powder is crossed 60 mesh sieves; The sterilising conditions of seed culture medium is: 121 ℃, and 30min;
3) plant the daughter bacteria actication of culture: in preceding 24 hours of kind of daughter bacteria cultivation, carry out the Bacillus subtilis strain activation of giving birth in the citrus; The amount of activated spawn switching slant tube press 5% of seed culture medium volume and is calculated, and 1 activated spawn adds the 10ml sterilized water and makes bacteria suspension, is inoculated into the bacteria suspension of careless genus bacillus living in the citrus that seed culture medium obtains activating; The condition of actication of culture is: 30 ℃, cultivate 24h;
4) inoculation: press the bacteria suspension that 5% of seed culture medium volume inserts the careless genus bacillus that gives birth in the activated citrus, shake up;
5) shake-flask culture: be that 160rpm shakes in the bottle at rotating speed, cultivate 24h down for 37 ℃ and obtain kind of a daughter bacteria.
8. the preparation method of organoselenium crop alimentary according to claim 6 agent is characterized in that, ferment tank, and concrete steps are as follows:
1) making of fermentation substrate: the preparation of raw material according to following weight parts becomes fermentation substrate, and raw material is: 6 parts of bean cake powders, 0.3 part in molasses, KH
2PO
491.7 parts of 2 parts, murphy juice; Described bean cake powder is crossed 60 mesh sieves;
2) fermentation substrate sterilising conditions: 121 ℃, 30min, the cooling back is standby;
3) inoculation kind of daughter bacteria: 10% inoculation kind of the daughter bacteria of pressing the fermentation substrate volume;
4) fermentation condition: 250rpm, 37 ℃, fermentation time 72h;
5) filtering fermentation liquor: after fermentation is finished, fermented liquid is filtered with 100 mesh sieves, namely obtain the dregs of beans amino acid solution.
9. according to the preparation method of claim 6 or 7 described organoselenium crop alimentary agent, it is characterized in that described subtilis is subtilis YS-45.
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