WO2016095481A1 - Small molecule peptide and allicin-containing tomato organic nutrient solution, and preparation method therefor - Google Patents

Small molecule peptide and allicin-containing tomato organic nutrient solution, and preparation method therefor Download PDF

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WO2016095481A1
WO2016095481A1 PCT/CN2015/082649 CN2015082649W WO2016095481A1 WO 2016095481 A1 WO2016095481 A1 WO 2016095481A1 CN 2015082649 W CN2015082649 W CN 2015082649W WO 2016095481 A1 WO2016095481 A1 WO 2016095481A1
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nutrient solution
allicin
tomato
preparation
small molecule
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PCT/CN2015/082649
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王猛
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苏州科大微龙信息技术有限公司
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation

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  • the invention relates to a tomato organic nutrient solution containing small molecular peptide and allicin and a preparation method thereof, and belongs to the technical field of organic fertilizers.
  • Tomato cultivation is generally carried out by soil culture, and there is a problem of flail in the planting process.
  • Tomato is a kind of vegetable and fruit that can be eaten in all seasons.
  • hydroponic and aerosol cultivation techniques to meet the market.
  • the purpose, but the finished product, the taste and quality are not as traditional as those cultivated by soil culture.
  • the tomato planted by hydroponics and aerosol culture makes the consumers worry.
  • the object of the present invention is to provide a tomato organic nutrient solution containing small molecular peptides and allicin and a preparation method thereof, which mainly use nutrients required for various tomato growth stages in raw materials, and have Allicin in garlic, and the active minerals contained in it, especially the garlic cultivated by our company, contain trace amounts of bio-selenium, active minerals of strontium, cellulose in potato stems and leaves, rice stalks, and citrus peel.
  • Special flavonoids, Hu The plant growth regulator of radish, limonene, and the small molecule peptide prepared by photocatalysis of spirulina have the function of cell repair.
  • the solid organic fertilizer with humic acid is decomposed at high temperature, and then hydrolyzed. Forming various ionic nutrients in the raw material containing fulvic acid complex, and obtaining the nutrient solution of the whole nutrients and the tomato organic nutrient solution of allicin by the action of the above active enzymes, thereby allowing the tomato plants to grow and develop healthily.
  • the hydroponic tomato that achieves the organic planting method can enhance its growth, increase the flavor rate and quality of the tomato finished product, and achieve the purpose of organic hydroponic tomato.
  • the present invention is a tomato organic nutrient solution containing a small molecular peptide and allicin and a preparation method thereof, which are characterized in that it is made of the following raw materials by weight: 30% of potato stems and leaves, eggshell 10 %, garlic 20%, straw 30%, citrus peel 10%, spirulina solution 10%; among them, fermentation bacteria group I: 20% by high temperature actinomycetes, 10% of Aspergillus oryzae, 10% of Aspergillus niger, 5% of , Bacillus subtilis 5%, Bacillus amyloliquefaciens 10%, Bacillus lentus 5%, Bacillus licheniformis 10%, Bacillus licheniformis 5%, Candida cerevisiae 10%, Candida utilis 10% .
  • the preparation process is as follows:
  • step (3) After removing the black mud from the completion of the step (2), adding the filtered water, and statically placing it for 3 days, and the water is formed into a yellowish brown liquid state;
  • step (3) the 400 mesh screen after the completion of step (3) is completed, and the residue is filtered to obtain a liquid.
  • step (4) the material of step (4) will be completed, and then the spirulina solution after photocatalysis is added, and after stirring and mixing, a tomato organic nutrient solution containing small molecular peptide and allicin is obtained.
  • the invention adopts the agricultural waste organic waste as a raw material, and after high-temperature high-speed solid-state fermentation, the spirulina extract after photocatalytic rupture is added, and the active growth required for the whole growth period of the tomato is provided.
  • Nutrients more importantly, fulvic acid, which is obtained by high-temperature fermentation, is used to absorb the active ionic elements after enzymatic hydrolysis, and has the characteristics of being quickly absorbed by tomatoes, and the biological growth regulator containing allicin and small molecule peptides. It can inhibit various pathogens in water, and there is no safety problem in material extraction.
  • the nutrient solution prepared by the invention belongs to the state of active enzyme and is easily absorbed by tomato, and can produce high quality organic tomato products. Achieve the mode of organic cultivation of hydroponic tomatoes.
  • 1 is a flow chart of preparation of a tomato organic nutrient solution containing a small molecular peptide and allicin;
  • the raw materials include 400 kg of potato stems and leaves, 130 kg of eggshells, 260 kg of garlic, 400 kg of straw, 130 kg of citrus peel, 100 ml of spirulina solution, and 1 kg of fermentation bacteria;
  • step (4) mixing: the material of step (4) is completed, and then the spirulina solution after photocatalysis is added, and after stirring and mixing, a tomato organic nutrient solution containing small molecular peptide and allicin is obtained.

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
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  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
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Abstract

Disclosed are a small molecule peptide and allicin-containing tomato organic nutrient solution, and a preparation method therefor. The nutrient solution is made from raw materials having the following weight percentages: 30% of potato stems and leaves, 10% of eggshells, 20% of garlic, 30% of straw, 10% of citrus peel, 10% of a spirulina solution, and 0.1% of fermentation microbiota I. In the preparation method, the agricultural residue organic material is crushed, fermentation microbiota I is added, high temperature high speed solid-state fermentation and composting are performed for 24 hours, the spirulina solution is added, and uniform mixing and stirring are performed. The small molecule peptide and allicin-containing tomato organic nutrient solution provides nutritional requirements for the whole growth period of a tomato, is non-toxic and tasteless, and contains small molecule peptides and allicin as biotransformation enzymes, present in the form of amino acids, and having antibacterial and cell repair functions. The nutrient solution prevents tomato diseases, reduces fruit splitting and deformed fruit, increases lycopene, and improves product quality and appearance.

Description

一种含小分子肽与大蒜素的番茄有机营养液及其制备方法Tomato organic nutrient solution containing small molecular peptide and allicin and preparation method thereof 技术领域Technical field
本发明涉及一种含小分子肽与大蒜素的番茄有机营养液及其制备方法,属于有机肥料技术领域。The invention relates to a tomato organic nutrient solution containing small molecular peptide and allicin and a preparation method thereof, and belongs to the technical field of organic fertilizers.
背景技术Background technique
番茄种植一般采用土培方式种植,存在种植过程中连茬的问题,番茄是一种可以四季食用的蔬菜水果,如何终年的供应市场,现在有水培与气雾培的种植技术,可以满足市场的需求,但目前用农业设施进行番茄无土栽培,为了补充番茄全生长期需要的营养,一般采用化学肥料,由水解的方式,浸泡成营养液,再加入植物生长激素,虽然可以达到终年供应的目的,但是种植出来的成品,口感、质量都不及传统以土培方式种植,在现在食品安全的问题上,水培与气雾培所种植出来的番茄,安全上面让消费者起到疑虑,因此制约了番茄水培与气雾培的市场发展,如何应用水培方式栽种,改变现在营养液配方,形成有机营养液,达到计划性的终年供应,又能实现有机种植的高质量番茄,是现代有机肥料领域技术人员,值得研究的方向。Tomato cultivation is generally carried out by soil culture, and there is a problem of flail in the planting process. Tomato is a kind of vegetable and fruit that can be eaten in all seasons. How to supply the market all the year round, now there are hydroponic and aerosol cultivation techniques to meet the market. The demand, but currently using agricultural facilities for tomato soilless cultivation, in order to supplement the nutrients needed for tomato throughout the growing season, generally using chemical fertilizer, by hydrolysis, soaked into nutrient solution, and then add plant growth hormone, although it can reach the year-round supply The purpose, but the finished product, the taste and quality are not as traditional as those cultivated by soil culture. On the issue of food safety nowadays, the tomato planted by hydroponics and aerosol culture makes the consumers worry. Therefore, it restricts the market development of tomato hydroponics and aerosol culture, how to apply hydroponics to plant, change the current nutrient solution formula, form organic nutrient solution, achieve the planned annual supply, and realize the high quality tomato planted organically. The technicians in the field of modern organic fertilizers are worthy of research.
发明内容Summary of the invention
针对现有的技术不足,本发明的目的是提供一种含小分子肽与大蒜素的番茄有机营养液及其制备方法,主要应用原料中的各种番茄生长期所需要的营养素外,另具有大蒜中的大蒜素、及其所含的活性矿物质,尤其经本公司栽培的大蒜,含有微量的生物硒、锗的活性矿物质,土豆茎叶、稻秆中的纤维素、柑橘皮中的特殊的黄酮类、胡 萝卜素、柠檬烯的植物生长调节素,螺旋藻经光催化制成的小分子肽,具有细胞修护的功能,经过发酵菌群,在高温下腐熟具有腐殖酸的固态有机肥,再经水解成含黄腐酸络合原料中的各种离子态营养素,由上述的物活性酶共同的作用下,得到全营养的小分子肽与大蒜素的番茄有机营养液,从而让番茄植株健康生长发育,达到有机种植方式的水培番茄,可以增进其生长,与结果率、增加番茄产成品的风味与品质,达到有机水培番茄的目的。In view of the deficiencies of the prior art, the object of the present invention is to provide a tomato organic nutrient solution containing small molecular peptides and allicin and a preparation method thereof, which mainly use nutrients required for various tomato growth stages in raw materials, and have Allicin in garlic, and the active minerals contained in it, especially the garlic cultivated by our company, contain trace amounts of bio-selenium, active minerals of strontium, cellulose in potato stems and leaves, rice stalks, and citrus peel. Special flavonoids, Hu The plant growth regulator of radish, limonene, and the small molecule peptide prepared by photocatalysis of spirulina have the function of cell repair. After fermenting the flora, the solid organic fertilizer with humic acid is decomposed at high temperature, and then hydrolyzed. Forming various ionic nutrients in the raw material containing fulvic acid complex, and obtaining the nutrient solution of the whole nutrients and the tomato organic nutrient solution of allicin by the action of the above active enzymes, thereby allowing the tomato plants to grow and develop healthily. The hydroponic tomato that achieves the organic planting method can enhance its growth, increase the flavor rate and quality of the tomato finished product, and achieve the purpose of organic hydroponic tomato.
1、为实现上述的目的本发明为一种含小分子肽与大蒜素的番茄有机营养液及其制备方法其特征在于由下列的重量百分比的原料制成:土豆茎叶30%、蛋壳10%、大蒜20%、秸秆30%、柑橘皮10%、螺旋藻液10%组成;其中发酵菌群Ⅰ:由高温放线菌20%、紫红曲霉10%、黑曲霉10%、大毛霉5%、枯草芽孢杆菌5%、解淀粉芽孢杆菌10%、萎缩芽孢杆菌5%、高地芽孢杆菌10%、食苯芽孢杆菌5%、乳酒假丝酵母10%、产脘假丝酵母10%所组成。1. In order to achieve the above object, the present invention is a tomato organic nutrient solution containing a small molecular peptide and allicin and a preparation method thereof, which are characterized in that it is made of the following raw materials by weight: 30% of potato stems and leaves, eggshell 10 %, garlic 20%, straw 30%, citrus peel 10%, spirulina solution 10%; among them, fermentation bacteria group I: 20% by high temperature actinomycetes, 10% of Aspergillus oryzae, 10% of Aspergillus niger, 5% of , Bacillus subtilis 5%, Bacillus amyloliquefaciens 10%, Bacillus lentus 5%, Bacillus licheniformis 10%, Bacillus licheniformis 5%, Candida cerevisiae 10%, Candida utilis 10% .
其制备的工艺如下:The preparation process is as follows:
(1)、将收集的土豆茎叶、蛋壳、大蒜、秸秆、柑橘皮,依据材料配比粉碎成40目的细末后,投入高温高速发酵釜;(1) The collected potato stems and leaves, eggshells, garlic, straw, and citrus peel are pulverized into 40-mesh fines according to the ratio of the materials, and then placed in a high-temperature high-speed fermentation kettle;
(2)、投入发酵菌群Ⅰ,经110℃,24H固态发酵,腐熟成黑色泥状物;(2), into the fermenting bacteria group I, after 110 ° C, 24H solid state fermentation, decomposed into a black mud;
(3)、将完成步骤(2)的黑色泥状物取出后,加入过滤后的水,静态放置3d,待水形成黄褐色液态状;(3) After removing the black mud from the completion of the step (2), adding the filtered water, and statically placing it for 3 days, and the water is formed into a yellowish brown liquid state;
(4)、将完成步骤(3)完成后的经400目筛网,过滤残渣,取得液 态物,经紫外线灭菌;(4), the 400 mesh screen after the completion of step (3) is completed, and the residue is filtered to obtain a liquid. State, UV-sterilized;
(5)、将完成步骤(4)的材料,再加入经光催化后的螺旋藻液,搅拌混合后,即得一种含小分子肽与大蒜素的番茄有机营养液。(5), the material of step (4) will be completed, and then the spirulina solution after photocatalysis is added, and after stirring and mixing, a tomato organic nutrient solution containing small molecular peptide and allicin is obtained.
本发明的优点:Advantages of the invention:
与现有的技术相比,本发明采用农余有机废弃物为原料,经过高温高速固态发酵后,再添加经光催化破壁后的螺旋藻萃取液、具备番茄全生长期所需要的活性生长营养素,更重要的经由高温发酵完成的黄腐酸,用以吸收原料经酶解后的活性离子态元素,具备快速被番茄吸收的特性,所含的大蒜素、小分子肽的生物生长调节剂,可以抑制水中的各种病原体,材料取材上无安全问题,本发明所制取的营养液,属于为活性酶的状态存在,容易被番茄吸收使用,可以生产出高质量的有机番茄产成品,实现水培番茄有机种植的模式。Compared with the prior art, the invention adopts the agricultural waste organic waste as a raw material, and after high-temperature high-speed solid-state fermentation, the spirulina extract after photocatalytic rupture is added, and the active growth required for the whole growth period of the tomato is provided. Nutrients, more importantly, fulvic acid, which is obtained by high-temperature fermentation, is used to absorb the active ionic elements after enzymatic hydrolysis, and has the characteristics of being quickly absorbed by tomatoes, and the biological growth regulator containing allicin and small molecule peptides. It can inhibit various pathogens in water, and there is no safety problem in material extraction. The nutrient solution prepared by the invention belongs to the state of active enzyme and is easily absorbed by tomato, and can produce high quality organic tomato products. Achieve the mode of organic cultivation of hydroponic tomatoes.
附图说明DRAWINGS
图1为一种含小分子肽与大蒜素的番茄有机营养液的制备流程图;1 is a flow chart of preparation of a tomato organic nutrient solution containing a small molecular peptide and allicin;
具体实施方式detailed description
下面结合附图1详细说明本发明的一种含小分子肽与大蒜素的番茄有机营养液的制备流程,以生产1000千克的营养液说明:The preparation process of a tomato organic nutrient solution containing a small molecule peptide and allicin according to the present invention will be described in detail below with reference to FIG. 1 to produce a 1000 kg nutrient solution:
(1)、备料:备齐原料包括土豆茎叶400千克、蛋壳130千克、大蒜260千克、秸秆400千克、柑橘皮130千克、螺旋藻液100ml组成;发酵菌群1千克;(1) Preparation: The raw materials include 400 kg of potato stems and leaves, 130 kg of eggshells, 260 kg of garlic, 400 kg of straw, 130 kg of citrus peel, 100 ml of spirulina solution, and 1 kg of fermentation bacteria;
(2)、固态发酵:将土豆茎叶、蛋壳、大蒜,秸秆、柑橘皮的材料 经粉碎机,粉成40目细末后,投入高温高速发酵釜中、投入发酵菌群Ⅰ,经24H,110℃发酵腐熟成黑色泥状物;(2), solid state fermentation: the material of potato stems and leaves, eggshells, garlic, straw, citrus peel After being crushed into 40 mesh fine powder, it is put into a high-temperature high-speed fermentation kettle, and is put into fermenting bacteria group I, and fermented into black mud by 24H, 110 °C fermentation;
(3)、水解:将完成步骤(2)的黑色泥状物取出,放入玻璃罐中,加入以淹没材料10cm高度的水,静置放置3d水解,得黄褐色液体;(3) Hydrolysis: The black mud which completed the step (2) was taken out, placed in a glass jar, and water was added to the height of 10 cm of the submerged material, and left to stand for 3 d to obtain a yellow-brown liquid;
(4)、过滤:将完成步骤(3)水解后的原料,经过400目网筛,过滤固体残渣;经紫外线灭菌;(4), filtration: the raw material after the completion of the step (3) is hydrolyzed through a 400 mesh sieve, and the solid residue is filtered;
(5)、混合:将完成步骤(4)的材料,再加入经光催化后的螺旋藻液,搅拌混合后,即得一种含小分子肽与大蒜素的番茄有机营养液。(5), mixing: the material of step (4) is completed, and then the spirulina solution after photocatalysis is added, and after stirring and mixing, a tomato organic nutrient solution containing small molecular peptide and allicin is obtained.
虽然本发明已以较佳实施例揭示如上,然其并非用以限定本发明,任何本领域技术人员,在不脱离本发明的精神和范围内,当可作些许的修改和完善,因此本发明的保护范围当以权利要求书所界定的为准。 While the present invention has been described in its preferred embodiments, the present invention is not intended to be limited thereto, and the present invention may be modified and improved without departing from the spirit and scope of the invention. The scope of protection is defined by the terms of the claims.

Claims (2)

  1. 一种含小分子肽与大蒜素的番茄有机营养液及其制备方法,其特征在于由下列的重量百分比的原料制成:土豆茎叶30%、蛋壳10%、大蒜20%、秸秆30%、柑橘皮10%、螺旋藻液10%、发酵菌群Ⅰ0.1%组成;发酵菌群:由高温放线菌20%、紫红曲霉10%、黑曲霉10%、大毛霉5%、枯草芽孢杆菌5%、解淀粉芽孢杆菌10%、萎缩芽孢杆菌5%、高地芽孢杆菌10%、食苯芽孢杆菌5%、乳酒假丝酵母10%、产脘假丝酵母10%所组成。Tomato organic nutrient solution containing small molecular peptide and allicin and preparation method thereof, which is characterized by being made of the following raw materials by weight: 30% of potato stems and leaves, 10% of eggshells, 20% of garlic, 30% of straw 10% citrus peel, 10% spirulina solution, 0.1% fermenting bacteria group; fermenting bacteria group: 20% high temperature actinomycetes, 10% red Monascus, 10% Aspergillus niger, 5% of Rhizopus oryzae, Bacillus subtilis Bacillus 5%, Bacillus amyloliquefaciens 10%, Bacillus lentus 5%, Bacillus licheniformis 10%, Bacillus licheniformis 5%, Candida cerevisiae 10%, Candida utilis 10%.
  2. 为实现权力要求1的一种含小分子肽与大蒜素的番茄有机营养液及其制备方法如下:A tomato organic nutrient solution containing the small molecule peptide and allicin for fulfilling claim 1 and a preparation method thereof are as follows:
    (1)、将收集的土豆茎叶、蛋壳、大蒜、秸秆、柑橘皮,依据材料配比粉碎成40目的细末后,投入高温高速发酵釜;(1) The collected potato stems and leaves, eggshells, garlic, straw, and citrus peel are pulverized into 40-mesh fines according to the ratio of the materials, and then placed in a high-temperature high-speed fermentation kettle;
    (2)、投入发酵菌群Ⅰ,经110℃,24H固态发酵,腐熟成黑色泥状物;(2), into the fermenting bacteria group I, after 110 ° C, 24H solid state fermentation, decomposed into a black mud;
    (3)、将完成步骤(2)的黑色泥状物取出后,加入过滤后的水,静态放置3d,待水形成黄褐色液态状;(3) After removing the black mud from the completion of the step (2), adding the filtered water, and statically placing it for 3 days, and the water is formed into a yellowish brown liquid state;
    (4)、将完成步骤(3)完成后的经400目筛网,过滤残渣,取得液态物;(4), the 400 mesh screen after the completion of step (3) is completed, and the residue is filtered to obtain a liquid material;
    (5)、将完成步骤(4)的材料,再加入经光催化后的螺旋藻液,搅拌混合后,即得一种含小分子肽与大蒜素的番茄有机营养液。 (5), the material of step (4) will be completed, and then the spirulina solution after photocatalysis is added, and after stirring and mixing, a tomato organic nutrient solution containing small molecular peptide and allicin is obtained.
PCT/CN2015/082649 2014-12-16 2015-06-29 Small molecule peptide and allicin-containing tomato organic nutrient solution, and preparation method therefor WO2016095481A1 (en)

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