CN108911851A - A kind of efficient foliage bacterial fertilizer of Japanese plum and the preparation method and application thereof - Google Patents
A kind of efficient foliage bacterial fertilizer of Japanese plum and the preparation method and application thereof Download PDFInfo
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- CN108911851A CN108911851A CN201810861551.5A CN201810861551A CN108911851A CN 108911851 A CN108911851 A CN 108911851A CN 201810861551 A CN201810861551 A CN 201810861551A CN 108911851 A CN108911851 A CN 108911851A
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- fertilizer
- japanese plum
- microbial inoculum
- plum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The invention discloses efficient foliage bacterial fertilizer of a kind of Japanese plum and the preparation method and application thereof, 10-20 parts of microbial inoculum containing germ oligotrophy unit cell, 8-12 parts of providencia stuartii microbial inoculum, 3-7 parts of monospore category bacteria agent, 6-10 parts of chitosan, 6-10 parts of amino acid, 8-12 parts of seaweeds, micro- 10-20 parts of fertilizer, 20-30 parts of water.Applied to the yield of increase plum, improvement plum quality, improve plum storage performance, induction enhancing Japanese plum disease resistance, the growth of promotion Japanese plum plant etc..Have many advantages, such as significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease, improves yield and quality, is low in cost and easy to spread.
Description
Technical field
The present invention relates to Japanese plum cultivation techniques and fertilising field, and in particular to a kind of efficient foliage bacterial fertilizer of Japanese plum and its preparation
Method and application.
Background technique
Plum (Prunus salicina Lindl.) is rosaceae cherry, alias Jiaqing, cloth continuous heavy rain, plum, jade
Emperor Lee, mountain plum.Mature between its 7~August of fruit, full mellow and full, exquisitely carved, form is pretty and dazzling, and it is that people most like that taste is sweet
One of joyous fruit.Cultivation extensively all over the world.Plum is sour, can promote the secretion of gastric acid and gastric juice, and can promote stomach
Enterocinesia, thus have improvement appetite, promote the effect of digestion, especially to glutted after gastric anacidity, food, constipation person is effective.
The amino acid such as the serine, glycine, proline, glutamine in fresh Lee's meat, there is inducing diuresis to remove edema, have to cirrhosis auxiliary
Help therapeutic effect.Contain multiple nutritional components in plum, plays the role of skin maintenance and cosmetic benefits, lubricates the skin and muscle, antioxidant contains in plum
It measures high surprising, is rated as " the super fruit " of anti-aging, anti-disease.
But with the development of industry, administrative skill level is backward, the outstanding problems such as serious, yield and quality is low occur for pest and disease damage
It presents, and then it is extremely urgent to establish high-quality, efficient, ecological, safe Japanese plum corresponding cultivation techniques.Chemical fertilizer is low in agricultural production
Utilization rate and the high environmental risk of its bring are always to perplex the significant problem in the whole world.Foliage bacterial fertilizer is by being sprayed onto plant leaf surface
After can be absorbed by plant rapidly by plant leaf blade, avoid from nutrient when soil application easily by soil consolidation, root
Portion absorbs the problems such as slow, has many advantages, such as to absorb fast, nutrient loss less.Currently, being mentioned there are many kinds of existing foliage bacterial fertilizers
Also there is certain effect in the yield of high crop, but to improve crop yield effect not significantly equal scarce enough for all different degrees of presence
It falls into and there are no the ideal foliage bacterial fertilizers designed exclusively for Japanese plum in the prior art.
Summary of the invention
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, meets Japanese plum at significant effect
The efficient foliage bacterial fertilizer of the Japanese plum of growing nutrient demand.
Technical scheme is as follows：
A kind of efficient foliage bacterial fertilizer of Japanese plum, it is characterised in that：It is made from the following raw materials in parts by weight：Thermophilic malt oligotrophy list
10-20 parts of born of the same parents' bacteria agent, 8-12 parts of providencia stuartii microbial inoculum, 3-7 parts of monospore category bacteria agent, 6-10 parts of chitosan, ammonia
6-10 parts of base acid, 8-12 parts of seaweeds, micro- 10-20 parts of fertilizer, 20-30 parts of water.
Preferably, it is made from the following raw materials in parts by weight：15 parts of germ oligotrophy unit cell microbial inoculum, Si Shi Providian
It is 10 parts of this bacteria agent, 5 parts of monospore category bacteria agent, 8 parts of chitosan, 8 parts of amino acid, 10 parts of seaweeds, fertile 15 parts, 29 parts of water micro-.
Preferably, the strain of the germ oligotrophy unit cell microbial inoculum is germ oligotrophy unit cell XQ08 (CCTCC NO:
M 2015726), the strain of providencia stuartii microbial inoculum be providencia stuartii JD (CCTCC NO:M
2017738), the strain of monospore category bacteria agent is monospore category bacterium J3 (CCTCCNO:M2017739).
Wherein, the microbial inoculum preparation includes the following steps：(1) inclined-plane strain X Q08, JD and J3 is saved into test tube seed, point
It is not seeded in minimal medium, shaken cultivation to logarithmic growth phase, obtains bacterium solution；(2) by the bacterium solution of acquisition with 10%
The inoculum concentration of percent by volume is seeded in the seed fermentation tank containing minimal medium, and culture to logarithmic growth phase obtains
Seed liquor；(3) seed liquor of acquisition is inoculated in fermentation medium according to the inoculum concentration of 10% volume ratio, 30-35 DEG C,
Fermented and cultured 2-3 days under 150-200rpm, obtain fermentation liquid, the living bacteria count of fermentation liquid >=10,000,000,000/mL, by fermentation liquid
Directly 5-20 times of dilution obtains germ oligotrophy unit cell liquid bacterial agent, providencia stuartii liquid bacterial agent and list respectively
Spore category bacteria liquid microbial inoculum.
Further, the chitosan is water solubility, and deacetylation is greater than 95%；The amino acid is proline, sweet ammonia
Acid, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine,
Valine, alanine, serine, arginine, asparatate, any five kinds or more of combination in cysteine；Seaweed
Element is pure natural marine algae extract；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:
Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer of 0.5-1；The water is sterile water.
The preparation method of the efficient foliage bacterial fertilizer of Japanese plum of the invention, comprises the technical steps that：
Step 1. takes germ oligotrophy unit cell liquid bacterial agent, Si Shi Providian described in claim 4 by regulation proportion
This bacteria liquid microbial inoculum and monospore category bacteria liquid microbial inoculum, it is spare；
Step 2. is matched by regulation takes chitosan, amino acid, seaweeds and micro- fertilizer that the nothing for being dissolved in required proportion is sufficiently stirred
In bacterium water；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH6.0~6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to Japanese plum blade face bacterial fertilizer products.
Bio-bacterial manure of the invention can be applied in following (1)-(4) at least one：
(1) increase the yield of Japanese plum；(2) improve Japanese plum quality, improve Japanese plum storage performance；(3) induction enhancing Japanese plum is anti-
Characteristic of disease, the diseases such as effective prevention and control Japanese plum shothole disease, heart-eating worm, bleeding disease, root knot, rot disease；(4) promote the life of Japanese plum plant
It is long；
Compared with prior art, the present invention foliage bacterial fertilizer of the present invention uses germ oligotrophy unit cell liquid bacterial agent, Si Shi
The composition such as Providence liquid bacterial agent, monospore category bacteria liquid microbial inoculum, plant growth-promoting substance, a great number of elements and microelement,
Japanese plum economic benefit, nutrient and flavour quality and storage performance can be effectively improved, promotes plant well to grow, and can effectively induce Lee
Plant, the enhancing of fruit disease resistance are set, there is significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease pest, improve yield
Quality, it is low in cost and easy to spread the advantages that.
Specific embodiment
Below with reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1：
1. bio-bacterial manure composition and preparation：
Bio-bacterial manure A formula composition and preparation, (1) composition：15 parts of germ oligotrophy unit cell microbial inoculum, Si Shi Providian
It is 10 parts of this bacteria agent, 5 parts of monospore category bacteria agent, 8 parts of chitosan, 8 parts of amino acid, 10 parts of seaweeds, fertile 15 parts, 29 parts of water micro-.
(2) it prepares：1. taking germ oligotrophy unit cell liquid bacterial agent, providencia stuartii liquid bacterial agent and monospore by regulation proportion
Belong to bacteria liquid microbial inoculum, it is spare；2. taking chitosan, amino acid, seaweeds and micro- fertilizer to be sufficiently stirred by regulation proportion is dissolved in required match
In the sterile water of ratio；3. 1. microbial inoculum that step obtains is added in 2. solution that step obtains, in 25-30 DEG C of temperature, pH 6.0-
Under conditions of 6.8, it is sufficiently mixed in sterile blender uniformly, it is packed up to the efficient foliage bacterial fertilizer A of Japanese plum.
Foliar fertilizer B formula composition and preparation, (1) composition：8 parts of chitosan, 8 parts of amino acid, 10 parts of seaweeds, micro- fertile 15
Part, 59 parts of water.(2) it prepares：1. taking chitosan, amino acid, seaweeds and micro- fertilizer to be sufficiently stirred by regulation proportion is dissolved in required match
In the sterile water of ratio, Japanese plum foliar fertilizer B is made.
2. field trial：
Experimental field overview：Experimental field it is located at Zhenning Guizhou Japanese plum garden, kind is " bee sugar Lee ".It is for examination orchard soil type
Yellow earth, as soil background, pH value is random in orchard before test, multiple spot mixing acquisition depth 0-60cm soil sample
5.65, total nitrogen content 1.45gkg-1, content of organic matter 29.81gkg-1, alkaline hydrolysis nitrogen content is 98.67mgkg-1,
Available phosphorus content is 7.43mgkg-1。
Field experimental design：RANDOMIZED BLOCK DESIGN is taken in test, using complete stool (including blade face front and back sides, limb, fruit
Deng) spraying, if 100 times of 100-200 times of liquid of foliage bacterial fertilizer, foliar fertilizer liquid, spraying clear water as control (CK), totally 3 processing, every place
5 trees are managed, are repeated 4 times, totally 12 cells, shield row is around set.Administration time is Japanese plum leaf stretching period, setting phase, nutriment product
The tired phase applies respectively, and amount of application is respectively 30,45,60kg/ mus.Weather is based on fine during test, or it is cloudy, after spraying
Without rainfall in 3-4 days.
3. analysis project and method：
After fruit maturation, is harvested by cell, measure each cell single plant yield, 200 fruits of each cell random acquisition,
Take back experimental determination fruit quality, single fruit weight.Nutrient quality measuring method：Dry matter is measured with National Standard Method；Vitamin C content
It is measured with 2,6 dichlorophenol indophenol method；Titratable acid (TA) content determination of acid-basetitration；Soluble solid (SSC) content is used
The measurement of PAL-1 type refractometer.Storage time is all rotted the time using stored Lee.
Incidence and maturation history：Starting softening in fruit is every 2d investigation fruit incidence, counts accumulative morbidity
Rate；And investigate Japanese plum plant diseases incidence.Disease incidence (%)=100 × (hair the number of sick fruits/investigation the total number of fruits) or disease incidence
(%)=100 × (morbidity strain number/investigation strain number).The growth indexes such as Japanese plum plant leaf SPAD value are observed 7-8 month in fertilising.
4. embodiment effect：
From table 1 to table 3 as can be seen that can effectively increase single plant yield, average fruit using the efficient foliage bacterial fertilizer of Japanese plum
Weight significantly improves fruit dry matter, vitamin C and soluble solid (SSC) content, reduces titratable acid (TA) content, promotees
Into the formation of plum yield and the improvement of quality；The generation such as plum disease can also be effectively reduced；Effectively facilitate the life of Japanese plum plant
It is long.
Table 1 applies influence of the foliage bacterial fertilizer to Japanese plum yield and quality
Note：It indicates with different lowercases are marked after column data in P<0.05 lower difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 lower difference is same up to extremely significant horizontal following table
Table 2 applies foliage bacterial fertilizer, and to Japanese plum disease, a situation arises
3 Japanese plum garden of table uses foliage bacterial fertilizer plant growth condition
It is above specific application example of the invention, there are other embodiments of the invention, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection scope of the presently claimed invention.
Claims (6)
1. a kind of efficient foliage bacterial fertilizer of Japanese plum, it is characterised in that：It is made from the following raw materials in parts by weight：Thermophilic malt oligotrophy unit cell
10-20 parts of bacteria agent, 8-12 parts of providencia stuartii microbial inoculum, 3-7 parts of monospore category bacteria agent, 6-10 parts of chitosan, amino
6-10 parts sour, 8-12 parts of seaweeds, micro- 10-20 parts of fertilizer, 20-30 parts of water.
2. the efficient foliage bacterial fertilizer of Japanese plum according to claim 1, it is characterised in that：It by following parts by weight raw material system
At：15 parts of germ oligotrophy unit cell microbial inoculum, 10 parts of providencia stuartii microbial inoculum, 5 parts of monospore category bacteria agent, chitosan 8
It is part, 8 parts of amino acid, 10 parts of seaweeds, fertile 15 parts, 29 parts of water micro-.
3. the efficient foliage bacterial fertilizer of Japanese plum according to claim 1 or 2, it is characterised in that：The germ oligotrophy unit cell
The strain of microbial inoculum is germ oligotrophy unit cell XQ08, the strain of providencia stuartii microbial inoculum is providencia stuartii
JD, monospore category bacteria agent strain be monospore category bacterium J3；The microbial inoculum preparation includes the following steps：(1) by strain X Q08, JD and
The inclined-plane J3 saves test tube seed, is seeded in minimal medium respectively, shaken cultivation to logarithmic growth phase, obtains bacterium solution；(2)
The bacterium solution of acquisition is seeded in the seed fermentation tank containing minimal medium with the inoculum concentration of 10% percent by volume, is trained
It supports to logarithmic growth phase, obtains seed liquor；(3) seed liquor of acquisition is inoculated with according to the inoculum concentration of 10% volume ratio into fermentation
In culture medium, fermented and cultured 2-3 days at 30-35 DEG C, 150-200rpm obtains fermentation liquid, and the living bacteria count of fermentation liquid is equal
Fermentation liquid is directly diluted 5-20 times respectively and obtains general sieve of germ oligotrophy unit cell liquid bacterial agent, Si Shi by >=100 hundred million/mL
Wei Dengsi bacteria liquid microbial inoculum and monospore category bacteria liquid microbial inoculum.
4. the efficient foliage bacterial fertilizer of Japanese plum according to claim 1 or 2, it is characterised in that：The chitosan is water solubility, is taken off
Acetyl degree is greater than 95%；The amino acid is proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, relies ammonia
Acid, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate,
Any five kinds or more of combination in cysteine；The seaweeds are pure natural marine algae extract；Micro- fertilizer is quality
Than for:3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer of 0.5-1
+ selenium fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer；The water is sterile water.
5. a kind of preparation method of the efficient foliage bacterial fertilizer of Japanese plum as claimed in claim 1 or 2, it is characterised in that：Including as follows
Processing step：
Step 1. takes germ oligotrophy unit cell liquid bacterial agent, providencia stuartii described in claim 4 by regulation proportion
Liquid bacterial agent and monospore category bacteria liquid microbial inoculum, it is spare；
Step 2. is matched by regulation takes chitosan, amino acid, seaweeds and micro- fertilizer that the sterile water for being dissolved in required proportion is sufficiently stirred
In；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., the item in 25-30 DEG C of temperature, pH6.0~6.8
Under part, it is sufficiently mixed in sterile blender uniformly, it is packed up to Japanese plum blade face bacterial fertilizer products.
6. a kind of application of the efficient foliage bacterial fertilizer of Japanese plum as claimed in claim 1 or 2, it is characterised in that including following side
The application in face：(1) increase the yield of Japanese plum；(2) improve Japanese plum quality, improve Japanese plum storage performance；(3) induction enhancing Japanese plum is anti-
Characteristic of disease, disease of effective prevention and control including Japanese plum shothole disease, bleeding disease, root knot, rot disease；(4) promote Japanese plum plant
Growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810861551.5A CN108911851A (en) | 2018-08-01 | 2018-08-01 | A kind of efficient foliage bacterial fertilizer of Japanese plum and the preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810861551.5A CN108911851A (en) | 2018-08-01 | 2018-08-01 | A kind of efficient foliage bacterial fertilizer of Japanese plum and the preparation method and application thereof |
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| Publication Number | Publication Date |
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| CN108911851A true CN108911851A (en) | 2018-11-30 |
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| CN201810861551.5A Withdrawn CN108911851A (en) | 2018-08-01 | 2018-08-01 | A kind of efficient foliage bacterial fertilizer of Japanese plum and the preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112745157A (en) * | 2020-10-30 | 2021-05-04 | 广西壮族自治区农业科学院 | Selenium-enriched biological foliar fertilizer and preparation method and application thereof |
-
2018
- 2018-08-01 CN CN201810861551.5A patent/CN108911851A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112745157A (en) * | 2020-10-30 | 2021-05-04 | 广西壮族自治区农业科学院 | Selenium-enriched biological foliar fertilizer and preparation method and application thereof |
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Application publication date: 20181130 |