CN103110013A - Low-protein daily ration for growing pig under net energy system and application thereof - Google Patents
Low-protein daily ration for growing pig under net energy system and application thereof Download PDFInfo
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- CN103110013A CN103110013A CN2013100585260A CN201310058526A CN103110013A CN 103110013 A CN103110013 A CN 103110013A CN 2013100585260 A CN2013100585260 A CN 2013100585260A CN 201310058526 A CN201310058526 A CN 201310058526A CN 103110013 A CN103110013 A CN 103110013A
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Abstract
The invention discloses low-protein daily ration for a growing pig under a net energy system and an application thereof, and belongs to the field of feed processing. The method comprises the steps of: taking the growing pig with the weight being 20-50kg as a research object, utilizing corn-bean pulp daily ration, reducing the protein content of the daily ration by 2% on the basis of the standard recommended by the National Research Council, simultaneously supplying lysine, methionine, threonine and tryptophan; adding mannan oligosaccharide, multivitamins, complex phosphoesterasum, probiotics and the like to form the low-protein daily ration for the growing pig under the net energy system; crushing, mixing, pelletizing then dosing, and packaging the pelletized feed and a probiotic component in a mixing manner to obtain the product disclosed by the invention. The animal feed utilization rate can be improved; the use quantity of antibiotic medicines in the feeding of animals is effectively reduced; the immunity of fed animals is improved; the security of animal meat products is improved; and the raise efficiency is also improved. The low-protein daily ration is scientific in process design, high in production efficiency, low in cost, and suitable for multi-variety and small-scale production.
Description
Technical field
The invention belongs to field of feed processing, be specifically related to formula Design of growth pig low-protein diet under the net energy system and preparation method thereof.
Background technology
In the proteinaceous nutrient theory, animal from being in essence the utilization to amino acid (amino acids, AA), as long as daily ration provides rational amino acid, just can be satisfied the protein requirement (Li Defa, 2003) of animal to the demand of protein.But be subjected to industrial the impact, the preparation of tradition daily ration can only ensure amino acid whose demand by protein level, cause the reasonable utilization of feed resource to be restricted, the major protein feedstuff too concentrates in the use of dregs of beans, and allow the pollutant component excretion such as nitrogen phosphorus increase due to nutrition unbalanced, bring huge pressure to feeding environment.The application of synthesizing amino acid, make nutrient supply more balanced, help to improve the utilization rate of protein, bring possibility for reduction forage protein raw material consumption and Dietary Crude Protein (crude protein, CP) level, can reduce elimination of nitrogen amount in feeding process when guaranteeing largest production efficient, reduced the harmful effect that environmental problem causes, and can reasonably combined feed resource, utilize unconventional raw material, promoted new feed resource rational exploitation and utilization.
Current, the low-protein diet theory has had a lot of achievements in research, and has important practice significance and wide application prospect in Swine Production.But in present research, great majority only concentrate on the reduction degree of dietary protein level and the addition of synthesizing amino acid, and to the rare report of the relation of protein in low-protein diet and energy level.Dietary ME and appraisement system are the focuses of Nutritional studies always, especially net energy (net energy, NE) system is in the application of swine rations preparation, bring opportunity for traditional nutrition theoretical developments, but existing report seldom there is system to carry out the further research of this system for low-protein diet.In low-protein diet, the foundation of the equilibrium relation of energy and protein and net energy system is new problem and the new challenge that faces during pig industry is produced.
Summary of the invention
The present invention is take 20-50kg growth pig as research object, use corn-soybean meal diet, dietary protein content is reduced by 2 percentage points on the NRC proposed standard, the net energy level is set, while lysine supplement, methionine, threonine and tryptophan, and add manna oligosacchride, B B-complex, complex enzyme, probiotic etc. and form growth pig low-protein diet under the net energy systems, comprise the raw material of following parts by weight:
Corn 66-71 part; Wheat bran 3-6 part; Dregs of beans 16-20 part; Soya-bean oil 0.3-0.6 part; Calcium monohydrogen phosphate 0.8-1.2 part; Stone flour 0.3-0.6 part; Salt 0.3-0.5 part; L lysine HCL 0.2-0.4 part; L-Trp 0.02-0.05 part; DL-METHIONINE 0.1-0.3 part; L-threonine 0.1-0.3 part; Manna oligosacchride 0.05-0.1 part; High temperature resistant phytase 0.01-0.02 part; Complex enzyme 0.03-0.05 part; B B-complex 0.3-0.4 part; Composite trace element 0.2-0.6 part; Probiotic 1-3 part.
The parts by weight of described complex enzyme consist of: arabinoxylanase 26-30 part; Proteinase 8-12 part; Mannase 6-10 part; α-amylase 8-12 part; Zinc chloride 2-3 part; Calcium chloride 1-3 part; Sodium sulphate 1-2 part.
In described B B-complex, the parts by weight of each component consist of: vitamin A 9-10 part; Vitamin D
33-4 part; Vitamin e1 1-12 part; Vitamin K
31-2 part; Cobastab
10.4-0.8 part; Cobastab
22-3 part; Cobastab
61.5-2.3 part; Cobastab
120.5-1.0 part; Niacinamide 16-17 part; Calcium pantothenate 4-5 part; Biotin 0.5-1.0 part.
The parts by weight of described composite trace element consist of;
Zinc sulfate 3-5 part; Ferrous sulfate 20-30 part; Basic copper chloride 1-2 part; Amino acid chelate copper 2-3 part; Manganese sulfate 6-8 part; Yeast chromium 0.5-1.5 part; Yeast selenium 0.5-1.5 part; Calcium iodate 3-4 part; Cobalt chloride 0.5-1.5 part.
The parts by weight of described each component of probiotic are: Lactobacillus plantarum microbial inoculum 1-3 part, Bacillus acidi lactici microbial inoculum 2-4 part, saccharomyces cerevisiae microbial inoculum 1-3 part.
The preparation of saccharomyces cerevisiae bacteria agent: slant strains obtains yeast starter liquid through the multistage technique that spreads cultivation of routine, and switching enters in fermentation tank, and controlling temperature is 28 ℃, and early stage, aerlbic culture was 14 hours, and throughput is controlled to be 2.0m
3/ minute, the later stage anaerobism was cultivated 15 hours; Zymotic fluid low temperature is concentrated, and after carrier mixed, through the fluidized bed drying preparation, vehicle group became CaCO
340 parts, 20 parts, dextrin, 20 parts of corn protein powders.Adding mass ratio in the saccharomycetes to make fermentation culture medium is the rear 40-50 purpose Radix Angelicae Sinensis excessively of pulverizing of 2-6% and the rear 40-50 of mistake of the pulverizing purpose Radix Isatidis that mass ratio is 3-8%.
Lactobacillus plantarum microbial inoculum preparation: the inclined-plane is cultivated the Lactobacillus plantarum seed liquor and is transferred in fermentation tank, and controlling temperature is 28-32 ℃, and anaerobism was cultivated 22 hours, and throughput is 2.0m
3/ minute; The complete centrifugation of fermenting obtains wet thallus, adds the protective agent that consists of 8% defatted milk, 4% trehalose, 5% glycerine and 5% glucose, obtains microbial inoculum by freeze drying, and moisture is lower than 10%.
Bacillus acidi lactici microbial inoculum preparation: the inclined-plane is cultivated Bacillus acidi lactici seed liquid and is transferred in fermentation tank, and controlling temperature is 28-32 ℃, and anaerobism was cultivated 22 hours, and throughput is 2.0m
3/ minute; The complete centrifugation of fermenting obtains wet thallus, adds the protective agent that consists of defatted milk 10%, lactose 5%, glycerine 5% and 5% glucose, obtains microbial inoculum by freeze drying, and moisture is lower than 10%.
Growth pig low-protein diet under above-mentioned net energy system, under the condition of daily ration amino acid balance, crude protein content is 15.5-16%, but standard ileum digestible lysine net energy ratio is 1.00-1.04g/MJ, net energy content is 10.06-10.48MJ/kg.
The processing technology of product of the present invention is:
Adopt first pulverize after proportioning process, granulate after mixing, with the feed after granulating and the probiotic component is hybrid packed gets final product.
Beneficial effect:
1, net energy system, compare with the metabolizable energy system with digestible energy, provide more accurately animal keep and production utilize required can value, can react comparatively really that the growth pig really is used for keeping and the production purpose can value, accurate reliable foundation can be provided the preparation of swine rations; Reduce daily ration DE, ME, CP level and feed cost; Control energy deposition and trunk fat.
2, under the prerequisite of low-protein amino acid balance daily ration, further use the net energy system, but and make digestible lysine and net energy keep proper ratio, important prerequisite is established in the preparation of preparation high performance-price ratio daily ration.
3, the interpolation of manna oligosacchride can promote intestinal health, and later stage production performance and meat quality also increase; Can reduce the growth pig intestinal mucosa oxidation of amino acid and glucose is increased the outer tissue of intestines to its absorption, thereby improve the body utilization rate of amino acid and glucose.
4, the plant and animal proteinoid that protease can be in suitable Water Under solution feed, become peptide and free amino acid, can improve animal to the utilization ratio of protein, promote animal to the absorption of protein in feed, under the composite coordination effect of other enzyme preparations, promote the digestion of raw material, and then promote growing of animal.
5, add high temperature resistant phytase in the growth swine rations, crude protein and organic apparent digestibility can be obviously improved, the true digestibility of seven kinds of essential amino acids of terminal ileum (arginine, lysine, histidine, phenylalanine, leucine, methionine and isoleucine) and seven kinds of nonessential amino acid (glycine, proline, aspartic acid, glutamic acid, alanine tyrosine and threonine) can be effectively improved simultaneously.
6, technological design science, production efficiency is high, and cost is low, is suitable for many kinds, small lot batch manufacture.
7, the present invention has added the composite probiotic component of science; Lactobacillus plantarum microbial inoculum, Bacillus acidi lactici microbial inoculum, saccharomyces cerevisiae microbial inoculum.Lactobacillus plantarum microbial inoculum, Bacillus acidi lactici microbial inoculum and saccharomyces cerevisiae microbial inoculum adopt respectively preferred CGMCC1.555, CICC21007 and CICC31481.Product of the present invention has been realized organic assembling with Lactobacillus plantarum microbial inoculum, Bacillus acidi lactici microbial inoculum, saccharomyces cerevisiae microbial inoculum; Lactobacillus plantarum and Bacillus acidi lactici begin growth and breeding and produce lactic acid at body fluid with under trophic factors is assisted on every side, invention the plant bacillus of adopting and the distinctive strong galactopoiesis acidity of Bacillus acidi lactici brought into play, the generation of lactic acid promotes and forms the digestive environments of stomach effectively, enzyme preparation begins to have an effect, and decomposes wherein nutriment; Promote digesting and assimilating of nutriment; The living factor of the benefits such as product Bacillus acidi lactici of the present invention enters enteron aisle and can effectively reduce the usage quantity of antibiotics in letting animals feed, improve the immunity of letting animals feed, improve the security of animal meat product, improve the food utilization efficiency of animal, the use of antibiotic and medicine in the conventional raising of minimizing, strengthen the resistivity of animal, improve raise benefit.
The specific embodiment
Lactobacillus provided by the present invention is that Lactobacillus plantarum (Lactobacillus plantarum) CGMCC No.1.555 is purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center; Saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC31481, Bacillus acidi lactici (Lactobacillus) CICC21007 are purchased from Chinese industrial microorganism fungus kind preservation administrative center.
The preparation of Bacillus acidi lactici (Lactobacillus) microbial inoculum:
(1) first order seed is cultivated: in Bacillus acidi lactici slant strains access 500 ml shake flasks, and 100 milliliters of culture medium loading amounts, 180 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks 1000 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: with the first class seed pot of three grades of seeds take 10% inoculum concentration access total measurement (volume) as 150L, fermentation medium loading amount 100L, 28 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours;
(5) fermented and cultured: the first class seed pot bacterial classification is accessed total measurement (volume) as 1.5 tons of secondary seed tanks take 10% inoculum concentration, 1 ton of fermentation medium loading amount, 28 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours is cultivated and is finished bacteria concentration 5.0 * 10
8Individual/ml.
(6) centrifugation: adopt centrifuge to separate and obtain thalline.
(7) vacuum freeze drying: adopt drying process with atomizing to process the acquisition dry bacteria after adding protective agent; Protective agent consists of the aqueous solution of defatted milk 10%, lactose 5%, glycerine 5% and 5% glucose.Vacuum freeze-drying technique is write the culture presevation handbook referring to Institute of Microorganism, Academia Sinica, publishes in 1980. 610-653.
Culture medium forms: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, pH6.8.
The preparation of Lactobacillus plantarum agent:
(1) first order seed is cultivated: in Lactobacillus plantarum bacterial classification access 500 ml shake flasks, and 100 milliliters of culture medium loading amounts, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks 1000 milliliters of culture medium loading amounts, 30 ℃ of cultivation temperature, incubation time 24 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: with the first class seed pot of three grades of seeds take 5% inoculum concentration access total measurement (volume) as 150L, fermentation medium loading amount 100L, 30 ℃ of cultivation temperature, tank pressure 0.05Mpa, incubation time 18 hours;
(5) fermentation tank culture: the first class seed pot bacterial classification is accessed total measurement (volume) as 3 tons of secondary seed tanks take 5% inoculum concentration, 2 tons of fermentation medium loading amounts, 30 ℃ of condition of culture cultivation temperature, tank pressure 0.05Mpa, incubation time 24 hours is cultivated and is finished bacteria concentration 4.0 * 10
8Individual/ml.
(6) centrifugation: adopt centrifuge to separate and obtain thalline.
(7) vacuum freeze drying: add protective agent and adopt vacuum refrigeration to carry out dry treatment process, protective agent consists of the aqueous solution of 8% defatted milk, 4% trehalose, 5% glycerine and 5% glucose.Vacuum freeze-drying technique is write culture presevation handbook, 1980.610-653. referring to Institute of Microorganism, Academia Sinica
Culture medium consists of: casein peptone 1%, and beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamines 0.2%, Tween80 0.1%, K
2HPO
40.2%, MgSO
4.7H
2O0.02%, MnSO
4.H
2O0.005%, CaCO
32%, agar 1.5%, pH6.8.
The preparation of saccharomyces cerevisiae microbial inoculum: slant strains obtains yeast starter liquid through the multistage technique that spreads cultivation of routine, and switching enters in fermentation tank, and controlling temperature is 28 ℃, and early stage, aerlbic culture was 14 hours, and throughput is controlled to be 2.0m
3/ minute, the later stage anaerobism was cultivated 15 hours; Zymotic fluid low temperature is concentrated, and after carrier mixed, through the fluidized bed drying preparation, vehicle group became CaCO
340 parts, 20 parts, dextrin, 20 parts of corn protein powders.Adding mass ratio in the saccharomycetes to make fermentation culture medium is the rear 40-50 purpose Radix Angelicae Sinensis excessively of pulverizing of 2-6% and the rear 40-50 of mistake of the pulverizing purpose Radix Isatidis that mass ratio is 3-8%.Cultivation method for saccharomycete and culture medium adopt the cellar culture method.
Embodiment 1
(1) under a kind of net energy system, growth pig low-protein diet feed is comprised of the raw material of lower parts by weight:
66 parts of corns; 3 parts, wheat bran; 16 parts of dregs of beans; 0.3 part, soya-bean oil; 0.8 part of calcium monohydrogen phosphate; 0.3 part of stone flour; 0.3 part of salt; 0.2 part of L lysine HCL; 0.02 part of L-Trp; 0.1 part of DL-METHIONINE; 0.10 part of L-threonine; 0.05 part of manna oligosacchride; 0.01 part of high temperature resistant phytase; 0.03 part of complex enzyme; 0.3 part of B B-complex; 0.2 part of composite trace element; 1 part of probiotic.
The parts by weight of described complex enzyme consist of: 26 parts of arabinoxylanases; Proteinase 8 part; 6 parts of mannases; α-amylase 8 part; 2 parts of zinc chloride; 2 parts, calcium chloride; 1 part, sodium sulphate.
The parts by weight of described B B-complex consist of: 9 parts of vitamin As; Vitamin D
33 parts; 1 part of vitamin e1; Vitamin K
31 part; Cobastab
10.4 part; Cobastab
22 parts; Cobastab
61.5 part; Cobastab
120.5 part; 16 parts of niacinamide; 4 parts of calcium pantothenates; 0.5 part of biotin.
The parts by weight of described composite trace element consist of;
3 parts, zinc sulfate; 20 parts, ferrous sulfate; 1 part of basic copper chloride; 2 parts of amino acid chelate copper; 6 parts of manganese sulfates; 0.5 part of yeast chromium; 0.5 part of yeast selenium; 3 parts of calcium iodates; 0.5 part of cobalt chloride.
The parts by weight of described each component of probiotic are: 1 part of Lactobacillus plantarum microbial inoculum, 2 parts of Bacillus acidi lactici microbial inoculums, 1 part of saccharomyces cerevisiae microbial inoculum.
The preparation of saccharomyces cerevisiae bacteria agent: slant strains obtains yeast starter liquid through the multistage technique that spreads cultivation of routine, and switching enters in fermentation tank, and controlling temperature is 28 ℃, and early stage, aerlbic culture was 14 hours, and throughput is controlled to be 2.0m
3/ minute, the later stage anaerobism was cultivated 15 hours; Zymotic fluid low temperature is concentrated, and after carrier mixed, through the fluidized bed drying preparation, vehicle group became CaCO
340 parts, 20 parts, dextrin, 20 parts of corn protein powders.Add mass ratio and be 4% rear mistake 45 purpose Radix Angelicae Sinensis of pulverizing and mass ratio in the saccharomycetes to make fermentation culture medium and be 6% the rear mistake 40 purpose Radix Isatidis of pulverizing.
(2) preparation method: with above-mentioned raw materials by weight after feeding, to pulverize together with dregs of beans, wheat bran after corn impurity elimination de-iron, then adopt with other raw material and intermittently mix and corresponding hybrid mode, mix 3min, mixture homogeneity is controlled in 5%, color and luster uniformity after mixing.The material that mixes enters quality-adjusting device, and refining temperature is 60 ℃, 40 seconds modified time, enter after modified granulator granulate, cooling hybrid packed with the probiotic component after reaching room temperature.
Embodiment 2
(1) under a kind of net energy system, growth pig low-protein diet feed is comprised of the raw material of lower parts by weight:
68 parts of corns; 5 parts, wheat bran; 18 parts of dregs of beans; 0.5 part, soya-bean oil; 1 part of calcium monohydrogen phosphate; 0.5 part of stone flour; 0.5 part of salt; 0.3 part of L lysine HCL; 0.03 part of L-Trp; 0.2 part of DL-METHIONINE; 0.2 part of L-threonine; 0.07 part of manna oligosacchride; 0.01 part of high temperature resistant phytase; 0.04 part of complex enzyme; 0.3 part of B B-complex; 0.4 part of composite trace element; 2 parts of probiotics.
The parts by weight of described complex enzyme consist of: 28 parts of arabinoxylanases; 10 parts, protease; 8 parts of mannases; 10 parts of α-amylase; 2 parts of zinc chloride; 2 parts, calcium chloride; 1 part, sodium sulphate.
The parts by weight of described B B-complex consist of: 0 part of retinol1; Vitamin D
34 parts; 2 parts of vitamin e1s; Vitamin K
32 parts; Cobastab
10.5 part; Cobastab
23 parts; Cobastab
62.0 part; Cobastab
120.8 part; 17 parts of niacinamide; 5 parts of calcium pantothenates; 0.8 part of biotin.
The parts by weight of described composite trace element consist of;
4 parts, zinc sulfate; 25 parts, ferrous sulfate; 2 parts of basic copper chlorides; 3 parts of amino acid chelate copper; 7 parts of manganese sulfates; 1.0 parts of yeast chromiums; 1.0 parts of yeast seleniums; 4 parts of calcium iodates; 1.0 parts of cobalt chlorides.
The parts by weight of described each component of probiotic are: 2 parts of Lactobacillus plantarum microbial inoculums, 3 parts of Bacillus acidi lactici microbial inoculums, 2 parts of saccharomyces cerevisiae microbial inoculums.
The preparation of saccharomyces cerevisiae bacteria agent: slant strains obtains yeast starter liquid through the multistage technique that spreads cultivation of routine, and switching enters in fermentation tank, and controlling temperature is 28 ℃, and early stage, aerlbic culture was 14 hours, and throughput is controlled to be 2.0m
3/ minute, the later stage anaerobism was cultivated 15 hours; Zymotic fluid low temperature is concentrated, and after carrier mixed, through the fluidized bed drying preparation, vehicle group became CaCO
340 parts, 20 parts, dextrin, 20 parts of corn protein powders.Add mass ratio and be 2% rear mistake 40 purpose Radix Angelicae Sinensis of pulverizing and mass ratio in the saccharomycetes to make fermentation culture medium and be 8% the rear mistake 40 purpose Radix Isatidis of pulverizing.
(2) preparation method: with embodiment 1
Embodiment 3
(1) under a kind of net energy system, growth pig low-protein diet feed is comprised of the raw material of lower parts by weight:
71 parts of corns; 6 parts, wheat bran; 20 parts of dregs of beans; 0.6 part, soya-bean oil; 1.2 parts of calcium monohydrogen phosphates; 0.6 part of stone flour; 0.5 part of salt; 0.4 part of L lysine HCL; 0.05 part of L-Trp; 0.10 part of 0.3 part of L-threonine of DL-METHIONINE; 0.1 part of manna oligosacchride; 0.02 part of high temperature resistant phytase; 0.05 part of complex enzyme; 0.4 part of B B-complex; 0.6 part of composite trace element; 3 parts of probiotics.
The parts by weight of described complex enzyme consist of: 30 parts of arabinoxylanases; 12 parts, protease; 10 parts of mannases; 12 parts of α-amylase; 2 parts of zinc chloride; 2 parts, calcium chloride; 1 part, sodium sulphate.
The parts by weight of described B B-complex consist of: 0 part of retinol1; Vitamin D
34 parts; 2 parts of vitamin e1s; Vitamin K
32 parts; Cobastab
10.8 part; Cobastab
23 parts; Cobastab
62.3 part; Cobastab
121.0 part; 17 parts of niacinamide; 5 parts of calcium pantothenates; 1.0 parts of biotins.
The parts by weight of described composite trace element consist of;
5 parts, zinc sulfate; 30 parts, ferrous sulfate; 2 parts of basic copper chlorides; 3 parts of amino acid chelate copper; 8 parts of manganese sulfates; 1.5 parts of yeast chromiums; 1.5 parts of yeast seleniums; 4 parts of calcium iodates; 1.5 parts of cobalt chlorides.
The parts by weight of described each component of probiotic are: 3 parts of Lactobacillus plantarum microbial inoculums, 4 parts of Bacillus acidi lactici microbial inoculums, 3 parts of saccharomyces cerevisiae microbial inoculums.
The preparation of saccharomyces cerevisiae bacteria agent: slant strains obtains yeast starter liquid through the multistage technique that spreads cultivation of routine, and switching enters to be added with in the fermentation tank of fermentation medium, and controlling temperature is 28 ℃, and early stage, aerlbic culture was 14 hours, and throughput is controlled to be 2.0m
3/ minute, the later stage anaerobism was cultivated 15 hours; Zymotic fluid low temperature is concentrated, and after carrier mixed, through the fluidized bed drying preparation, vehicle group became CaCO
340 parts, 20 parts, dextrin, 20 parts of corn protein powders.Add mass ratio and be 6% rear mistake 50 purpose Radix Angelicae Sinensis of pulverizing and mass ratio in the saccharomycetes to make fermentation culture medium and be 3% the rear mistake 40 purpose Radix Isatidis of pulverizing.
(2) preparation method: with embodiment 1.
Growth pig low-protein diet under the net energy system of employing embodiment 1 preparation is done following checking.
1 test material
1.1 test period
Tested in May, 2011, finish in mid-June, 2011 to raise, last 38 days.
1.2 test site
Test is in the pig farm enforcement of Lingyuan, Liaoning kind.
1.3 experimental animal
Experimental animal is selected totally 300 of the long white three way cross sodium selenites of Du Luoke * Da Bai *.
1.4 test daily ration
Two control groups and test group totally 3 processing are established in test.The control group feed is the corn-soybean meal growth pig feed of the rich production of Lingyuan standing grain, and the present invention describes is growth pig low-protein diet under the net energy system, adopts the described feed of example 1.
The control group daily ration forms
| Raw material | Control group one (parts by weight) | Control group two (parts by weight) |
| Corn | 66.00 | 71.40 |
| Dregs of beans | 25.00 | 19.50 |
| Wheat bran | 5.00 | 4.50 |
| Soya-bean oil | 1.00 | 1.00 |
| Stone flour | 0.60 | 0.50 |
| Calcium monohydrogen phosphate | 1.00 | 1.20 |
| Salt | 0.40 | 0.40 |
| Premix | 1.00 | 1.00 |
| L lysine HCL | 0.00 | 0.25 |
| L-threonine | 0.00 | 0.10 |
| DL-METHIONINE | 0.00 | 0.12 |
| L-Trp | 0.00 | 0.03 |
Annotate:
1Net energy calculates according to the equation of (1994) such as Noblet.
2Premix provides for every kilogram of daily ration: vitamin A, 5,512IU; Vitamin D
3, 2,200IU; Vitamin E, 64IU; Vitamin K
3, 2.2mg; Cobastab
12, 27.6 μ g; Riboflavin, 5.5mg; D-VB5,13.8mg; Nicotinic acid, 30.3mg; Choline Chloride, 551mg; Manganese, 40mg; Iron, 100mg; Zinc, 100mg; Copper, 100mg; Iodine, 0.3mg; Selenium, 0.3mg.
3In cost of material, corn, 46% dregs of beans, wheat bran, one-level soya-bean oil are respectively by 2400,4000,1800 and 12000 yuan/tons of calculating.
2 test methods
2.1 experimental design
Single factor Random Design is adopted in this test, select average initial body weight be about the Du Luoke of 22.96 ± 2.72kg * length in vain * totally 300 of Da Bai three way cross healthy growth pigs, by the close principle similar with hereditary basis of body weight, be divided at random 3 processing, the three kinds of different daily rations of feeding respectively.Each processes 10 repetitions, 10 pigs of each repetition (circle), and male and female half and half, the experimental subjects body weight is 20-50kg.38 days experimental periods, record feed intake, the weighing test is initial and the end is heavy, and calculates daily gain, daily ingestion amount and material anharmonic ratio and other indexs.
2.2 feeding and management
Test pig is raised in closed pig house, cement flooring, well-ventilated.Duration of test every morning and afternoon each reinforced 1 time, free choice feeding and drinking-water, by the pig farm conventional program carry out disinfection, expelling parasite and immunity.
2.3 testing index
2.3.1 ration ingredient
Diet dry substance, thick protein, calcium and total phosphorus are measured with reference to the method that State Standard of the People's Republic of China GB/T6435-1986, GB/T6432-1994, GB/T6436-2002 and GB/T6437-2002 recommend respectively.
The daily ration sample is measured 15 seed amino acids and sulfur-containing amino acid by hydrochloric acid hydrolysis 24h with automatic amino acid analyzer (L-8800 of Hitachi, Japan) at 110 ℃ of lower 6mol/L hydrochloric acid hydrolysis 24h and 0 ℃ of lower performic oxidation 16h respectively; After hydrolysis 22h under 110 ℃, use high performance liquid chromatograph (Shimadzu LC-10A, Japan) to measure tryptophan with 4mol/L NaOH.
2.3.2 growth performance
Respectively at each on-test and when finishing morning on an empty stomach individuality weigh, to repeat (circle) as the unit record feed consumption rate, calculate average daily gain, average daily ingestion amount and feed and increase weight and compare.
2.4 statistical analysis
After test data is carried out preliminary treatment with Excel software, adopt general linear model (general linearmodel, GLM) the modeling statistics analysis (SAS, 2001) of SAS8.2.
3 results and analysis
Under the different-energy system, low-protein diet is to growth pig growth performance contrast table
| ? | Control group one | Control group two | Test group |
| Starting weight (kg) | 22.96 | 22.91 | 22.96 |
| End heavy (kg) | 52.45 | 51.86 | 53.00 |
| Average daily gain (g/d) | 776.00 | 762.00 | 791.00 |
| Average daily ingestion amount (g/d) | 1585.00 | 1553.00 | 1601.00 |
| Feed/weightening finish | 2.04 | 2.04 | 2.03 |
Result shows, control group and test group growth performance comprise does not have difference on feed intake, feedstuff-meat ratio, speed of growth statistics (P〉0.05); The average daily gain of the low-protein diet under the net energy system and feed/weightening finish all have superiority; Therefore the relatively front two groups of reduction 50kcal/kg of low-protein diet under the net energy system, digestible energy further save cost on energy feed;
Test shows: test group reduces by 75.3% than control group antibiotic dosage.Pig hair light has color and luster, and hair color takes on a new look very large compared with the control; Result shows that product of the present invention can improve the pig body immunocompetence, reduces antibiotic dosage, increases the cultivation quality and benefits.So from the overall economic efficiency analysis, the low-protein diet cost performance under the net energy system is better, under the surging situation of current cost of material, market popularization value is arranged more especially, be beneficial to the pig farm and reduce feed cost, improve the income of raising pigs.
Claims (10)
1. growth pig low-protein diet is comprised of the raw material of following parts by weight:
Corn 66-71 part; Wheat bran 3-6 part; Dregs of beans 16-20 part; Soya-bean oil 0.3-0.6 part; Calcium monohydrogen phosphate 0.8-1.2 part; Stone flour 0.3-0.6 part; Salt 0.3-0.5 part; L lysine HCL 0.2-0.4 part; L-Trp 0.02-0.05 part; DL-METHIONINE 0.1-0.3 part; L-threonine 0.1-0.3 part; Manna oligosacchride 0.05-0.1 part, high temperature resistant phytase 0.01-0.02 part; Complex enzyme 0.03-0.05 part; B B-complex 0.3-0.4 part; Composite trace element 0.2-0.6 part; Probiotic 1-3 part.
2. growth pig low-protein diet according to claim 1, is characterized in that the parts by weight of described complex enzyme consist of: arabinoxylanase 26-30 part; Proteinase 8-12 part; Mannase 6-10 part; α-amylase 8-12 part; Zinc chloride 2-3 part; Calcium chloride 1-3 part; Sodium sulphate 1-2 part.
3. growth pig low-protein diet according to claim 1, is characterized in that the parts by weight of described B B-complex consist of: vitamin A 9-10 part; Vitamin D
33-4 part; Vitamin e1 1-12 part; Vitamin K
31-2 part; Cobastab
10.4-0.8 part; Cobastab
22-3 part; Cobastab
61.5-2.3 part; Cobastab
120.5-1.0 part; Niacinamide 16-17 part; Calcium pantothenate 4-5 part; Biotin 0.5-1.0 part.
4. growth pig low-protein diet according to claim 1, is characterized in that the parts by weight of described composite trace element consist of;
Zinc sulfate 3-5 part; Ferrous sulfate 20-30 part; Basic copper chloride 1-2 part; Amino acid chelate copper 2-3 part; Manganese sulfate 6-8 part; Yeast chromium 0.5-1.5 part; Yeast selenium 0.5-1.5 part; Calcium iodate 3-4 part; Cobalt chloride 0.5-1.5 part.
5. growth pig low-protein diet according to claim 1, is characterized in that the parts by weight of described each component of probiotic are: Lactobacillus plantarum microbial inoculum 1-3 part, Bacillus acidi lactici microbial inoculum 2-4 part, saccharomyces cerevisiae microbial inoculum 1-3 part.
6. growth pig low-protein diet according to claim 5 is characterized in that described Lactobacillus plantarum microbial inoculum preparation: the inclined-plane is cultivated the Lactobacillus plantarum seed liquor and is transferred in fermentation tank, and controlling temperature is 28-32 ℃, and anaerobism was cultivated 22 hours, and throughput is 2.0m
3/ minute; The complete centrifugation of fermenting obtains wet thallus, adds the protective agent that consists of 8% defatted milk, 4% trehalose, 5% glycerine and 5% glucose, obtains microbial inoculum by freeze drying, and moisture is lower than 10%.
7. according to claim 1 with 5 described growth pig low-protein diets is characterized in that described Bacillus acidi lactici microbial inoculum preparation: the inclined-plane is cultivated Bacillus acidi lactici seed liquid and is transferred in fermentation tank, and controlling temperature is 28-32 ℃, and anaerobism was cultivated 22 hours, and throughput is 2.0m
3/ minute; The complete centrifugation of fermenting obtains wet thallus, adds the protective agent that consists of defatted milk 10%, lactose 5%, glycerine 5% and 5% glucose, obtains microbial inoculum by freeze drying, and moisture is lower than 10%.
8. according to claim 1 with 5 described growth pig low-protein diets are comprised of the raw material of following parts by weight:
71 parts of corns; 6 parts, wheat bran; 20 parts of dregs of beans; 0.6 part, soya-bean oil; 1.2 parts of calcium monohydrogen phosphates; 0.6 part of stone flour; 0.5 part of salt; 0.4 part of L lysine HCL; 0.05 part of L-Trp; 0.10 part of 0.3 part of L-threonine of DL-METHIONINE; 0.1 part of manna oligosacchride; 0.02 part of high temperature resistant phytase; 0.05 part of complex enzyme; 0.4 part of B B-complex; 0.6 part of composite trace element; 3 parts of probiotics;
The parts by weight of described complex enzyme consist of: 30 parts of arabinoxylanases; 12 parts, protease; 10 parts of mannases; 12 parts of α-amylase; 2 parts of zinc chloride; 2 parts, calcium chloride; 1 part, sodium sulphate;
The parts by weight of described B B-complex consist of: 0 part of retinol1; 4 parts of cholecalciferols; 2 parts of vitamin e1s; Vitamin K
32 parts; Cobastab
10.8 part; Cobastab
23 parts; Cobastab
62.3 part; Cobastab
121.0 part; 17 parts of niacinamide; 5 parts of calcium pantothenates; 1.0 parts of biotins;
The parts by weight of described composite trace element consist of; 5 parts, zinc sulfate; 30 parts, ferrous sulfate; 2 parts of basic copper chlorides; 3 parts of amino acid chelate copper; 8 parts of manganese sulfates; 1.5 parts of yeast chromiums; 1.5 parts of yeast seleniums; 4 parts of calcium iodates; 1.5 parts of cobalt chlorides; The parts by weight of described each component of probiotic are: 3 parts of Lactobacillus plantarum microbial inoculums, 4 parts of Bacillus acidi lactici microbial inoculums, 3 parts of saccharomyces cerevisiae microbial inoculums.
9. according to claim 1 with 5 described growth pig low-protein diets is characterized in that adding in described saccharomycetes to make fermentation culture medium mass ratio and are 6% rear mistake 50 purpose Radix Angelicae Sinensis of pulverizing and mass ratio and be 3% the rear mistake 40 purpose Radix Isatidis of pulverizing.
10. according to claim 1-7 application of described growth pig low-protein diet in pig is fed.
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| C06 | Publication | ||
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Owner name: BEIJING HELLO ANIMAL SCIENCE CO., LTD. Free format text: FORMER OWNER: HEFENG LIVESTOCK FARMING CO., LTD., LIAONING Effective date: 20140529 |
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| C41 | Transfer of patent application or patent right or utility model | ||
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Inventor after: Yue Longyao Inventor after: Wang Dan Inventor after: Yao Qiaofen Inventor after: Shao Caimei Inventor before: Yue Longyao Inventor before: Shao Caimei Inventor before: Wang Dan |
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Free format text: CORRECT: INVENTOR; FROM: YUE LONGYAO SHAO CAIMEI WANG DAN TO: YUE LONGYAO WANG DAN YAO QIAOFEN SHAO CAIMEI Free format text: CORRECT: ADDRESS; FROM: 110164 SHENYANG, LIAONING PROVINCE TO: 102602 DAXING, BEIJING |
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Effective date of registration: 20140529 Address after: 102602 Beijing city Daxing District Yufa town planning area Sheng Ping Street No. 9 Applicant after: BEIJING HELAI TECHNOLOGY Co.,Ltd. Address before: 110164 Liaoning city of Shenyang province Shenbei New Area Huishan Street No. 169 Liaoning Hefeng animal husbandry Limited by Share Ltd Applicant before: Wellhope Foods Co.,Ltd. |
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Effective date of registration: 20230317 Address after: 110164 Liaoning city of Shenyang province Shenbei New Area Huishan Street No. 169 Patentee after: Hefeng Food Co.,Ltd. Address before: No. 9 Shengping Street, Guihua District, Yufa Town, Daxing District, Beijing 102602 Patentee before: BEIJING HELAI TECHNOLOGY Co.,Ltd. |