CN109497257A - A kind of beef cattle fermented feed and preparation method thereof for promoting lumen fermentation and improving immunity - Google Patents
A kind of beef cattle fermented feed and preparation method thereof for promoting lumen fermentation and improving immunity Download PDFInfo
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- CN109497257A CN109497257A CN201811258529.8A CN201811258529A CN109497257A CN 109497257 A CN109497257 A CN 109497257A CN 201811258529 A CN201811258529 A CN 201811258529A CN 109497257 A CN109497257 A CN 109497257A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses the beef cattle fermented feeds and preparation method thereof of a kind of promotion lumen fermentation and raising immunity, the feed is made of following components in parts by weight: 3-8 parts of dregs of beans, 8-25 parts of corn, 10-20 parts of fruits and vegetables slag, 0.5-5 parts of composite trace element function packet, 1-4 parts of vitamin function packet, 15-25 parts of immunologic facilitation function packet, 3-6 parts of calcium monohydrogen phosphate, 4-7 parts of magnesia, 2-5 parts of potassium chloride, 1-3 parts of composite fermentation strain, 5-10 parts of molasses, 20-30 parts of water.Product of the present invention is intended to promote function of rumen to stablize health by supplement probiotic, reinforced micro-element and vitamin function nutrition and immunopotentiator synergistic effect and improve beef cattle immunity.
Description
Technical field
The present invention relates to field of feed additive technology, and in particular to a kind of meat for promoting lumen fermentation and improving immunity
Ox fermented feed and preparation method thereof.
Background technique
Here statement only provides background information related with the disclosure, without necessarily constituting the prior art.
Beef cattle belongs to complex stomach animal, more than nonruminant three stomach rooms (reticulum, cud and omasums), wherein function of rumen
Health degree determines beef cattle breeding benefit.Lumen fermentation is a complicated process, and rumen microorganism is the main body of fermentation, specifically
Including bacterium, methanogen, fungi and protozoon, there are also a small number of bacteriophages.Beef cattle breeding key is especially existed in feed for nursing
The fattening period of beef cattle, fattening period need to guarantee that beef cattle intake meets the nutriment of its Fast Growth, not only can guarantee that beef cattle is strong
Kang Chengchang, also can guarantee the meat meat of beef cattle, and then reach the economic benefit of cultivation beef cattle.But in intensive culture condition
Under, due to long-term a large amount of feeding concentrated feeds, it is slow that lumen fermentation easily occurs, and for the health steady in a long-term for guaranteeing lumen fermentation, needs
Emphasis is wanted to solve rumen microorganism activity, pH value and oxidation-reduction potential.
It is many about the feed addictive of beef cattle in the prior art, for example " one kind is for improving beef ox fattening for Chinese patent
Chinese herb feed additive of performance and preparation method thereof, application method and application ", application number: CN201711400465.6, it is open
Number: CN107912616A.Disclosure of the invention Chinese herb feed additive is remarkably improved fattening later period beef cattle dry matter intake, day
Weight gain reduces feed-weight ratio；To the trend that Slaughter, carcass trait are improved, while unsaturated fatty acid in beef can be improved
With the content of amino acid.But these reports do not account for improving the fermenting property etc. of cud.
Therefore, lacking in the prior art a kind of can effectively improve lumen fermentation performance and low in cost easy to spread use
The preferable cattle feed of comprehensive performance.
Summary of the invention
For the above prior art, the object of the present invention is to provide the beef cattles of a kind of promotion lumen fermentation and raising immunity
With fermented feed and preparation method thereof, cud can be promoted healthy, feed fermentation is abundant, and the utilization efficiency of feed improves, beef cattle
Fast growing, while immunity of organisms can be improved again, and do not develop drug resistance, cure rate height, noresidue, without phytotoxicity, it is not dirty
Dye environment, can increase Abwehrkraft des Koepers, act on rapid, uneasy to recur, cheap advantage green safe environmental protection.
Product of the present invention is intended to through supplement probiotic, reinforced micro-element and vitamin function nutrition and immunologic facilitation
Synergistic effect promotes function of rumen to stablize health and improves beef cattle immunity simultaneously.
To achieve the above object, the present invention specifically uses following technical scheme:
In the first aspect of the invention, a kind of beef cattle for promoting lumen fermentation and improving immunity fermentation feeding is provided
Material, is made of following components in parts by weight:
3-8 parts of dregs of beans, 8-25 parts of corn, 10-20 parts of fruits and vegetables slag, 0.5-5 parts of composite trace element function packet, compound dimension life
1-4 parts of plain function packet, 15-25 parts of immunologic facilitation function packet, 3-6 parts of calcium monohydrogen phosphate, 4-7 parts of magnesia are 2-5 parts of potassium chloride, multiple
Close fermenting microbe 1-3 parts, 5-10 parts of molasses, 20-30 parts of water.
Wherein, the immunologic facilitation function packet is made of the raw material of following parts by weight: 5-15 parts of semen ziziphi spinosae, Folium sophorae 15-
40 parts, 10-25 parts of pine needle meal, 1-5 parts of dried orange peel, 1-5 parts of hawthorn, 2.5-10 parts of wrinkled giant hyssop, 2.5-10 parts of Radix Angelicae Sinensis, Radix Glycyrrhizae 2.5-10
Part, 2.5-10 parts of Radix Astragali, 1-5 parts of caulis sinomenii, Radix Ophiopogonis 2.5-10 part；
The composite fermentation strain is made of the raw material of following parts by weight: saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/
G) 15-30 parts, 20-40 parts of aspergillus niger (viable count: 10~2,000,000,000 CFU/g), enterococcus faecalis (viable count: 10~2,000,000,000 CFU/g)
20-35 parts.
In the second aspect of the invention, the beef cattle fermented feed of a kind of promotion lumen fermentation and raising immunity is provided
Preparation method, method includes the following steps:
(1) other components by the feed in addition to composite fermentation strain stir evenly to obtain feed original in parts by weight
Material；
(2) the composite fermentation strain bacterium solution after activation is seeded in the feedstuff of step (1) ferment and is cured
Promote lumen fermentation and improves the beef cattle fermented feed of immunity.
Preferably, the activation method of the composite fermentation strain are as follows:
The brown sugar water or molasses being added into composite fermentation strain carry out activating to obtain composite fermentation strain bacterium solution, control activation
Temperature is 30~35 DEG C；
Wherein, the mass ratio of the composite fermentation strain and brown sugar water or molasses is 1~3:20, the brown sugar water or molasses
Mass fraction be 2w/w%.
Preferably, the step (2) method particularly includes: will uniformly be sprayed on feed original after the dilution of composite fermentation strain bacterium solution
In material, uniformly rear control water content to be mixed is 25-40%.
Preferably, the fermentation curing uses normal fermentation mode, such as sealed fermenting or compost fermentation.
In the third aspect of the invention, above-mentioned fermented feed is provided and promotes beef cattle lumen fermentation in preparation and improves immune
Application in power feed.
In the fourth aspect of the invention, the method for a kind of promotion beef cattle lumen fermentation and raising immunity, the party are provided
Method includes the steps that beef cattle eats the promotion lumen fermentation and improves the beef cattle fermented feed of immunity.
Specific application method are as follows: before beef cattle weight 150kg, by natural wind dry sample restatement, additive amount is beef cattle with entirely
The 1w/w% of valence mixed feed is additionally added；In beef cattle weight 150kg to delivering for sale, by natural wind dry sample restatement, additive amount is meat
The 2w/w% of ox perfect compound feed is additionally added；First the feed and beef cattle perfect compound feed are sufficiently mixed, then with slightly
Feed uses after being sufficiently mixed.
The object of the present invention is to provide the beef cattle fermented feeds and its system of a kind of promotion lumen fermentation and raising immunity
Preparation Method can promote beef cattle cud healthy, and feed fermentation is abundant, and the utilization efficiency of feed improves, beef cattle fast growing, simultaneously
Immunity of organisms can be improved again, and do not develop drug resistance, cure rate height, noresidue, without phytotoxicity, free from environmental pollution, green peace
Loopful is protected, can increase Abwehrkraft des Koepers, acts on rapid, uneasy to recur, cheap advantage.
Product of the present invention is intended to through supplement probiotic, reinforced micro-element and vitamin function nutrition and immunologic facilitation
Agent synergistic effect, promoting function of rumen to stablize health, simultaneously improving beef cattle immunity achieves remarkable result simultaneously.
(1) this product can be such that cud function stablizes, and promote lumen fermentation.
Cud function directly affects feed digestion degree, and function improves fermentation sufficiently, and the speed of growth is fast.Lumen fermentation is one
The process of a complexity, rumen microorganism are the main bodys of fermentation, specifically include bacterium, methanogen, fungi and protozoon, and there are also a small number of
Bacteriophage.Lumen fermentation is also influenced by temperature, pH value, oxidation-reduction potential, osmotic pressure etc..Since long-term a large amount of feeding essences are raised
Material, the volatile fatty acid that lumen fermentation generates easily cause rumen ecology, and the present invention adds buffered pH value and redox
Substance-calcium monohydrogen phosphate, magnesia, potassium chloride, hawthorn, the semen ziziphi spinosae (containing a large amount of organic acid in hawthorn and semen ziziphi spinosae) of current potential
Deng, buffering lumen fermentation excess generate acid, maintain normal pH and osmotic pressure.PH value be can be effectively controlled 6.5 or so, guaranteed
The health steady in a long-term of lumen fermentation.This product is probiotic fermented product, and probiotics and metabolite, cud are rich in after fermentation
Micro organism quantity is big, and feed fermentation is more abundant, and mycoprotein yield is big.This kind adds multi-vitamins simultaneously, improves micro- life
Object activity, promotes lumen fermentation.
(2) product of the present invention strengthens immunity of organism, promotes premunition, highly effective and safe.
With carrying forward vigorously for green cultivation idea, the dynamics that country prohibits control chemical drug is increasing, while prevention and health care is seen
Thought also becomes more and more popular, the bioactive substances such as polysaccharide, saponins, alkaloids, volatile oil that Chinese medicine contains, tool
Play the role of enhancing body's immunity, herbal medicine additive is thus widely used in preventing, treating for livestock and poultry, or make
It is feed addictive with substitute antibiotics.First is that herbal medicine and fowl poultry immune are adjusted.Modern research shows that Chinese herbal medicine can be to body
Nerve, body fluid and cellular and molecular level carry out comprehensive adjusting, to play adjustment effect.Traditional Chinese veterinary medicine it is signified " just
Gas " concept, practical is exactly the immunity of body.Due to many Chinese medicines and prescription all contain glucoside, alkaloid, polysaccharide, organic sugar,
The immunologic active materials such as volatile oil, therefore it can activate the cell and immunity system of body, improve the disease resistance of body,
Achieve the purpose that disease preventing and treating.Second is that immunologic adjuvant.Immunologic adjuvant, also known as immunopotentiator, be about antigen or antigen simultaneously
Non-specifically change in injection animal body, or enhancing body is to the substance of the specific immune response of the antigen.Immunologic adjuvant
A variety of vaccine effects are often assisted, the effects of having reduction veterinary vaccines production cost, improve the protection of vaccine.Currently, both at home and abroad
It is at the early-stage to the research of herbal medicine immunologic adjuvant, and achieve part progress.
Immunologic facilitation function packet of the invention includes plurality of Chinese raw material, and the Chinese medicine material compatibility of selection is reasonable, through testing
Verifying, can improve the immunity of beef cattle, while can assist improving the fermenting property of beef cattle cud.
This product mainly adds the kinds of traditional Chinese medicines such as semen ziziphi spinosae, pine needle meal, dried orange peel, hawthorn, Radix Angelicae Sinensis, Radix Ophiopogonis, has no toxic side effect,
Do not develop drug resistance, cure rate height, noresidue, without phytotoxicity, it is free from environmental pollution, it is green safe environmental protection, can increase Abwehrkraft des Koepers,
Act on rapid, uneasy to recur, cheap advantage.Wherein, pine needle meal: contain phytocide and protein abundant, dimension
Raw element and microelement etc., can not only improve the disease resistance of beef cattle, and can also improve the fermenting property of beef cattle cud, improve
Efficiency of feed utilization, addition pine needle meal can also integrally reduce the cost of feed.Dried orange peel: meridian distribution of property and flavor: it is mild-natured, it is sweet in flavor.Converge to the spleen channel, stomach
Through.Effect and effect: promoting the circulation of qi helps digestion, spleen benefiting and stimulating the appetite, moves back newborn dissipate-swelling.Belong to digestants.Radix Ophiopogonis: meridian distribution of property and flavor: cold nature, sweet in flavor,
Slight bitter.Thoughts of returning home warp, lung channel, stomach meridian.Effect and effect: nourishing Yin and promoting production of body fluid, moistening lung and clearing away the heart-fire.Belong to the yin tonics classified under qi-restoratives medicine.
(3) product of the present invention daily gain is fast, and dressing percentage is high.
Multi-vitamins and microelement are added in product of the present invention.Microelement can be the work of a variety of digestive ferments of raising
Property, nutriment is improved in the digestibility of the small intestinal segment of beef cattle.Liposoluble vitamin such as vitamin A, vitamine D3 are strengthened calcium phosphorus and are inhaled
It receives, promotes skeleton development, widen beef cattle skeleton, promote growth.B family vitamin is generally acknowledged to not have to add in cattle feed
Add, but due to a large amount of intakes of concentrated feed, the B family vitamin that lumen fermentation generates is insufficient for body needs, therefore volume
Outer addition such as niacinamide can promote the digestion and absorption of nutriment, and daily gain is fast.
(4) selection of each raw material of additive of the present invention and the proportion dosage of each raw material are inventor's grinding by many years
Study carefully and obtained with practice, by inventor long-term research and practice, lacking or changing certain raw materials therein can't resolve
Ideal effect is not achieved in the specific technical problem of the present invention, and the emphasis solved the problems, such as changes.If proportion relation is not
Rationally, not only lumen fermentation efficiency is obstructed, and significantly reduces body's resistance to disease, and the speed of growth is slower.
(5) fermented feed of the invention has mellowness smell, and has weak acid taste, and palatability is good.
Detailed description of the invention
The Figure of description for constituting present invention a part is used to provide further understanding of the present invention, signal of the invention
Examples and descriptions thereof are used to explain the present invention for property, does not constitute improper limitations of the present invention.
Fig. 1 is the histogram that sample influences dry matter degradability.
Fig. 2 is the histogram that sample influences crude protein degradation rate.
Specific embodiment
It is noted that described further below be all exemplary, it is intended to provide further instruction to the present invention.Unless another
It indicates, all technical and scientific terms used herein has usual with general technical staff of the technical field of the invention
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to exemplary embodiments of the present invention.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation and/or their combination.
In conjunction with specific example, the present invention is further illustrated, and following instance is not right merely to the explanation present invention
Its content is defined.If the experiment actual conditions being not specified in embodiment, usually according to normal condition, or it is public according to sale
Take charge of recommended condition；Feedstuff as used in the following examples (such as it is magnesia, calcium monohydrogen phosphate, corn, dregs of beans, compound
Microelement, multi-vitamins, bacterial strain etc.), it is commercially available unless otherwise specified.Heretofore described meat
Ox full price batch refers to conventional full ration formulation (TMR), is not particularly limited, can be purchased by commercial sources in the present invention
It buys or prepares and obtain.
In a kind of typical embodiment of the present invention, the beef cattle use of a kind of promotion lumen fermentation and raising immunity is provided
Fermented feed is made of following components in parts by weight:
3-8 parts of dregs of beans, 8-25 parts of corn, 10-20 parts of fruits and vegetables slag, 0.5-5 parts of composite trace element function packet, compound dimension life
1-4 parts of plain function packet, 15-25 parts of immunologic facilitation function packet, 3-6 parts of calcium monohydrogen phosphate, 4-7 parts of magnesia are 2-5 parts of potassium chloride, multiple
Close fermenting microbe 1-3 parts, 5-10 parts of molasses, 20-30 parts of pure water.
Wherein, the fruits and vegetables slag is the by-product after vegetables or fruit deep processing, and parts by weight are natural wind dry weight.
In the optimal embodiment of the present invention, a kind of beef cattle fermentation for promoting lumen fermentation and improving immunity
Feed is made of following components in parts by weight:
5 parts of dregs of beans, 15 parts of corn, 12 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, multi-vitamins function packet 2.5
Part, 20 parts of immunologic facilitation function packet, 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia, 4 parts of potassium chloride, 1.5 parts of composite fermentation strain, molasses 6
Part, 22 parts of pure water.
In a preferred embodiment of the present invention, the composite trace element function packet by following parts by weight raw material group
At: 5-10 parts of copper sulphate (copper coin cellulose content 25%), 20-40 parts of ferrous sulfate (iron content 28%), manganese sulfate (manganese element
Content 31%) 15-30 parts, 15-25 parts of zinc sulfate (Zn-ef ficiency content 34%), calcium iodate (content of calcium constituent is 10%) 1-3
Part, 0.1-0.3 parts of sodium selenite (content of selenium element is 10%), cobalt chloride (content of cobalt element is 10%) 0.05-0.2
Part, 18-22 parts of Kelp Powder.
In the optimal embodiment of the present invention, the composite trace element function packet, by the raw material group of following parts by weight
At: 8 parts of copper sulphate (copper coin cellulose content 25%), 28 parts of ferrous sulfate (iron content 28%), manganese sulfate (manganese element content
31%) 23 parts, 20 parts of zinc sulfate (Zn-ef ficiency content 34%), 1.6 parts of calcium iodate (content of calcium constituent is 10%), sodium selenite
0.2 part of (content of selenium element is 10%), 0.1 part, 19.1 parts of Kelp Powder of cobalt chloride (content of cobalt element is 10%).
In a preferred embodiment of the present invention, the multi-vitamins function packet is made of the raw material of following parts by weight:
(500,000 is international single for 10 parts of vitamin A (50 million international units/g), 2-5 parts of vitamine D3 (50 million international units/g), vitamin E
Position/g) 40-60 parts, 8-10 parts of niacinamide, 5-8 parts of biotin, 4-8 parts of antioxidant, 8-15 parts of vitamin C, skin of beancurd powder 3-8
Part.
In the optimal embodiment of the present invention, the multi-vitamins function packet is made of the raw material of following parts by weight:
10 parts of vitamin A (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), vitamin E (50 million international units/
G) 50 parts, 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, 5 parts of skin of beancurd powder.
In a preferred embodiment of the present invention, the immunologic facilitation function packet is made of the raw material of following parts by weight: acid
5-15 parts of jujube kernel, 15-40 parts of Folium sophorae, 10-25 parts of pine needle meal, 1-5 parts of dried orange peel, 1-5 parts of hawthorn, 2.5-10 parts of wrinkled giant hyssop, Radix Angelicae Sinensis
2.5-10 parts, 2.5-10 parts of Radix Glycyrrhizae, 2.5-10 parts of Radix Astragali, 1-5 parts of caulis sinomenii, Radix Ophiopogonis 2.5-10 part.
In the optimal embodiment of the present invention, the immunologic facilitation function packet is made of the raw material of following parts by weight: acid
8 parts of jujube kernel, 35 parts of Folium sophorae, 20 parts of pine needle meal, 3 parts of dried orange peel, 5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, Radix Astragali 5
Part, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.
In a preferred embodiment of the present invention, the composite fermentation strain is made of the raw material of following parts by weight: wine brewing
15-30 parts of yeast (viable count: 10~2,000,000,000 CFU/g), aspergillus niger (viable count: 10~2,000,000,000 CFU/g) 20-40 parts, excrement intestines ball
20-35 parts of bacterium (viable count: 10~2,000,000,000 CFU/g).
In some embodiments of the present invention, the composite fermentation strain is made of the raw material of following parts by weight: wine brewing ferment
Female 25 parts of CICC1015 (viable count: 10~2,000,000,000 CFU/g), aspergillus niger CICC2041 (viable count: 10~2,000,000,000 CFU/g) 35
Part, 28 parts of enterococcus faecalis CICC20423 (viable count: 10~2,000,000,000 CFU/g).
Parts by weight of the invention are using natural wind dry weight as radix.
In the another typical embodiment of the present invention, the beef cattle use of a kind of promotion lumen fermentation and raising immunity is provided
Fermented feed preparation method, specifically includes the following steps:
(1) activation of strain: taking 1-3kg composite fermentation strain that the brown sugar water of 20kg 2% or molasses is added to activate 3 hours,
30 DEG C of water temperature or so.
(2) dilution of bacterium solution: by bacterium solution in upper step and about 300kg water mixed diluting.
(3) inoculation of strain: the zymocyte liquid after dilution being uniformly sprinkled upon step by step in 1 ton of feedstuff to be fermented,
Water content so after mixing is about 25-40%, control moisture standard be hold it is agglomerating, one touch it is i.e. broken.Humidity is not also
Excessive, the excessive fermentation time length of humidity is easy to turn sour, and causes feed palatability poor.
(4) fermentation curing: the feed after inoculation can be directly attached to polybag, plastic barrel or large cylinder by a kind of mode
In, sealed fermenting.Another way can be come with heap, and surface plastic covering cloth is sealed by fermentation, and packing again after fermenting successfully makes
With.Suitably adjust the length of fermentation time according to ambient temperature, summer temp 30 DEG C or so 2-3 days, winter temperature is at 10 DEG C
Left and right fermentation 5-7 days.
In the another typical embodiment of the present invention, provide above-mentioned fermented feed preparation promote beef cattle lumen fermentation and
Improve the application in immunity feed.
In the another typical embodiment of the present invention, the side of a kind of promotion beef cattle lumen fermentation and raising immunity is provided
Method, this method include the steps that beef cattle eats the promotion lumen fermentation and improves the beef cattle fermented feed of immunity.
Method particularly includes: before beef cattle weight 150kg, by natural wind dry sample restatement, additive amount is concentrated feed (as beef cattle
Full price batch is formulated as corn 57.8%, wheat bran 16%, dregs of beans 20%, cotton dregs 2%, digested tankage 2%, bone meal 1%, salt
0.6%, sodium bicarbonate 0.6%) 1% additional addition；In beef cattle weight 150kg to delivering for sale, by natural wind dry sample restatement, additive amount
2% for concentrated feed additionally adds；First this product and concentrated feed are sufficiently mixed, (roughage is silage corn with roughage
Be 1:1 mixing with the quality of clover) be sufficiently mixed after use, the mass ratio of the concentrated feed and roughage is 4:6.
Using foregoing invention product, promote cud health, feed fermentation is abundant, and the utilization efficiency of feed improves, and beef cattle is raw
It is long quickly while immunity of organisms to be improved again, and do not develop drug resistance, cure rate height, noresidue, without phytotoxicity, do not pollute
Environment, can increase Abwehrkraft des Koepers, act on rapid, uneasy to recur, cheap advantage green safe environmental protection.
By a large amount of practices and research of applicant, determine that the formula feeding beef cattle of the above proportion relation can significantly improve life
Long speed, daily gain dramatically increase, and disease incidence obviously lowers.And the feeding cost of this product is decreased obviously.
Finally optimization determines the beef cattle fermented feed of a kind of promotion lumen fermentation and raising immunity, is under
State component and weight composition:
3-8 parts of dregs of beans, 8-25 parts of corn, 10-20 parts of fruits and vegetables slag, 0.5-5 parts of composite trace element function packet, compound dimension life
1-4 parts of plain function packet, 15-25 parts of immunologic facilitation function packet, 3-6 parts of calcium monohydrogen phosphate, 4-7 parts of magnesia are 2-5 parts of potassium chloride, multiple
Close fermenting microbe 1-3 parts, 5-10 parts of molasses, 20-30 parts of pure water.The composite trace element function packet is by following parts by weight
Raw material composition: 5-10 parts of copper sulphate (copper coin cellulose content 25%), 20-40 parts of ferrous sulfate (iron content 28%), manganese sulfate
(content of calcium constituent is for 15-30 parts of (manganese element content 31%), 15-25 parts of zinc sulfate (Zn-ef ficiency content 34%), calcium iodate
10%) 1-3 parts, 0.1-0.3 parts of sodium selenite (content of selenium element is 10%), cobalt chloride (content of cobalt element is 10%)
0.05-0.2 parts, 18-22 parts of Kelp Powder.The multi-vitamins function packet is made of the raw material of following parts by weight: vitamin A
10 parts of (50 million international units/g), 2-5 parts of vitamine D3 (50 million international units/g), vitamin E (50 million international units/g) 40-
60 parts, 8-10 parts of niacinamide, 5-8 parts of biotin, 4-8 parts of antioxidant, 8-15 parts of vitamin C, 3-8 parts of skin of beancurd powder.It is described to exempt from
Epidemic disease is strengthened function to wrap and is made of the raw material of following parts by weight: 5-15 parts of semen ziziphi spinosae, 15-40 parts of Folium sophorae, 10-25 parts of pine needle meal,
1-5 parts of dried orange peel, 1-5 parts of hawthorn, 2.5-10 parts of wrinkled giant hyssop, 2.5-10 parts of Radix Angelicae Sinensis, 2.5-10 parts of Radix Glycyrrhizae, 2.5-10 parts of Radix Astragali, green wind
1-5 parts of rattan, Radix Ophiopogonis 2.5-10 part.The composite fermentation strain is made of the raw material of following parts by weight: saccharomyces cerevisiae (viable count:
10~2,000,000,000 CFU/g) 15-30 parts, aspergillus niger (viable count: 10~2,000,000,000 CFU/g) 20-40 parts, enterococcus faecalis (viable count: 10
~20 hundred million CFU/g) 20-35 parts.
In a preferred embodiment of the present invention, the beef cattle fermentation of a kind of promotion lumen fermentation and raising immunity is provided
Feed feeding method, this method comprises:, by natural wind dry sample restatement, additive amount is concentrated feed before beef cattle weight 150kg
1% additional addition；In beef cattle weight 150kg to delivering for sale, by natural wind dry sample restatement, additive amount is the 2% additional of fine fodder feed
Addition；First this product and concentrated feed are sufficiently mixed, used after being sufficiently mixed with roughage.
Beef cattle belongs to ruminant, the beef cattle after 2 monthly ages, and completely, beef cattle grows required nutriment for cud development
The overwhelming majority maintains the Rumen Internal Environment of health from lumen fermentation product, promotes mycoprotein and volatile fat
Acid yield maximizes, and improves daily gain, and by cud electrolyte buffer balancing technique, improves lumen fermentation efficiency.
In order to enable those skilled in the art can clearly understand technical solution of the present invention, below with reference to tool
The embodiment and the comparative example technical solution that the present invention will be described in detail of body.
Embodiment 1 has the screening of high fermenting property bacterial strain
1.1 materials and methods
Bacterial strain is used in 1.1 tests
Bacterial strain is used in the test of table 1
| Serial number | Strain name |
| 1 | Saccharomyces cerevisiae |
| 2 | Enterococcus faecium |
| 3 | Enterococcus faecalis |
| 4 | Bacillus subtilis |
| 5 | Lactobacillus plantarum |
| 6 | Lactobacillus acidophilus |
| 7 | Lactobacillus fermenti |
| 8 | Streptococcus thermophilus |
| 9 | Pediococcus pentosaceus |
| 10 | Candida utili |
| 11 | Lactobacillus reuteri |
| 12 | Lactobacillus casei |
| 13 | Aspergillus oryzae |
| 14 | Aspergillus niger |
The above bacterial strain can be obtained by conventional commercial approach, and expanded culture through inventor and be prepared into powder formulation, living
Property is 10~2,000,000,000 CFU/g.
1.2 test methods:
(1) culture medium, formula are prepared are as follows: 5 parts of dregs of beans, 15 parts of corn, 12 parts of fruits and vegetables slag, composite trace element function packet 3
Part, 2.5 parts of multi-vitamins function packet, 20 parts of immunologic facilitation function packet, 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia, 4 parts of potassium chloride,
1.5 parts of composite fermentation strain.The composite trace element function packet, is made of the raw material of following parts by weight: copper sulphate (copper
Content 25%) 8 parts, 28 parts of ferrous sulfate (iron content 28%), 23 parts of manganese sulfate (manganese element content 31%), zinc sulfate
(content of selenium element is for 1.6 parts of 20 parts of (Zn-ef ficiency content 34%), calcium iodate (content of calcium constituent be 10%), sodium selenite
10%) 0.2 part, 0.1 part, 19.1 parts of Kelp Powder of cobalt chloride (content of cobalt element is 10%).The multi-vitamins function packet,
It is made of the raw material of following parts by weight: 10 parts of vitamin A (50 million international units/g), vitamine D3 (50 million international units/g) 3
Part, 50 parts of vitamin E (50 million international units/g), 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C,
5 parts of skin of beancurd powder.The immunologic facilitation function packet, is made of the raw material of following parts by weight: 8 parts of semen ziziphi spinosae, 35 parts of Folium sophorae, pine needle
20 parts of powder, dried orange peel 3,5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.
Each raw material component is uniformly mixed, and is prepared into solid medium.
(2) activated strains: take 1-3g fermenting microbe that the brown sugar water of 20g 2% or molasses is added to activate 3 hours, 30 DEG C of water temperature
Left and right, the bacterium solution after activation is prepared, and be uniformly mixed with 300g water.
(3) it ferments: the bacterium solution in step (1) being uniformly seeded to the solid medium of 1kg step (1), in 30 DEG C of anaerobism
Fermentation 3 days.
1.3 measurement viable counts
1.2 after fermentation, takes 5g fermentation material using clear water repeated flushing, obtains the flushing liquor containing viable bacteria, mixes, take
1mL measures the viable count in cleaning solution.Aspergillus oryzae and aspergillus niger are using blood counting chamber progress monospore sub-count, other bacterial strains
Using plate count.
2 results and analysis
Table 2
The experimental results showed that saccharomyces cerevisiae, enterococcus faecalis, bacillus subtilis, Candida utilis ferment in 1.2 culture medium
Female and aspergillus niger growth and breeding situation is preferable, so tentatively selected saccharomyces cerevisiae, enterococcus faecalis, bacillus subtilis, production protein
Candida and aspergillus niger are as bacterial strain to be tested.
By antagonistic experiment find saccharomyces cerevisiae, enterococcus faecalis, bacillus subtilis, candida utili and aspergillus niger it
Between without Antagonistic reaction, by above-mentioned bacterial strain to be tested carry out it is compound prepare composite bacteria, trained referring to 1.2 test method
It supports, discovery saccharomyces cerevisiae, the compound and saccharomyces cerevisiae of aspergillus niger and enterococcus faecalis, aspergillus niger and bacillus subtilis are answered
It closes, three kinds of strain growth situations in two groups are be more good, with growing state when independent culture without significant difference, so by this
Two groups of composite bacterias determine saccharomyces cerevisiae, aspergillus niger and enterococcus faecalis as composite bacteria to be tested, through subsequent application experiment
The composite bacteria of composition is with excellent lumen fermentation ability and the immunity that can significantly improve beef cattle.
Embodiment 2
The beef cattle fermented feed for preparing a kind of promotion lumen fermentation and raising immunity, is by following components in parts by weight
Composition: 5 parts of dregs of beans, 15 parts of corn, 12 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, multi-vitamins function packet 2.5
Part, 20 parts of immunologic facilitation function packet, 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia, 4 parts of potassium chloride, 1.5 parts of composite fermentation strain, molasses 6
Part, 22 parts of pure water.The composite trace element function packet, is made of the raw material of following parts by weight: copper sulphate (copper coin cellulose content
25%) 8 parts, 28 parts of ferrous sulfate (iron content 28%), 23 parts of manganese sulfate (manganese element content 31%), zinc sulfate (zinc member
Cellulose content 34%) 20 parts, 1.6 parts of calcium iodate (content of calcium constituent is 10%), sodium selenite (content of selenium element is 10%)
0.2 part, 0.1 part, 19.1 parts of Kelp Powder of cobalt chloride (content of cobalt element is 10%).The multi-vitamins function packet, under
The raw material of column parts by weight forms: 10 parts of vitamin A (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), dimension
50 parts of raw element E (50 million international units/g), 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, skin of beancurd
5 parts of powder.The immunologic facilitation function packet, is made of the raw material of following parts by weight: 8 parts of semen ziziphi spinosae, 35 parts of Folium sophorae, pine needle meal 20
Part, dried orange peel 3,5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.The compound hair
Yeast-like fungi kind is made of the raw material of following parts by weight: 25 parts of saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/g), aspergillus niger (viable bacteria
Number: 10~2,000,000,000 CFU/g) 35 parts, 28 parts of enterococcus faecalis (viable count: 10~2,000,000,000 CFU/g).
A kind of beef cattle fermented feed preparation method for promoting lumen fermentation and improving immunity, including following step
It is rapid: (1) activation of strain: to take 1-3kg composite fermentation strain that the brown sugar water of 20kg 2% or molasses is added to activate 3 hours, water temperature
30 DEG C or so.(2) dilution of bacterium solution: by bacterium solution in upper step and about 300kg water mixed diluting.(3) inoculation of strain: after dilution
Zymocyte liquid be uniformly sprinkled upon in 1 ton of feedstuff to be fermented step by step, water content so after mixing is about 25-
40%, control moisture standard be hold it is agglomerating, one touch it is i.e. broken.Humidity is not also excessive, and the excessive fermentation time of humidity is long to be easy hair
Acid.(4) fermentation curing: accumulation mode ferments, and surface plastic covering cloth is sealed by fermentation, and drying to pack again after fermentation makes
With.Suitably adjust the length of fermentation time according to ambient temperature, summer temp 30 DEG C or so 2-3 days, winter temperature is at 10 DEG C
Left and right fermentation 5-7 days.
Application method are as follows: before beef cattle weight 150kg, by natural wind dry sample restatement, additive amount is concentrated feed (as beef cattle
Full price batch is formulated as corn 57.8%, wheat bran 16%, dregs of beans 20%, cotton dregs 2%, digested tankage 2%, bone meal 1%, salt
0.6%, sodium bicarbonate 0.6%) 1% additional addition；In beef cattle weight 150kg to delivering for sale, by natural wind dry sample restatement, additive amount
2% for concentrated feed additionally adds；First this product and concentrated feed are sufficiently mixed, (roughage is silage corn with roughage
Be 1:1 mixing with the quality of clover) be sufficiently mixed after use, the mass ratio of the concentrated feed and roughage is 4:6.
Embodiment 3
The beef cattle fermented feed for preparing a kind of promotion lumen fermentation and raising immunity, is by following components in parts by weight
Composition: 6 parts of dregs of beans, 18 parts of corn, 10 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, multi-vitamins function packet 2.5
Part, 18 parts of immunologic facilitation function packet, 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia, 3 parts of potassium chloride, 1.5 parts of composite fermentation strain, molasses 6
Part, 22 parts of pure water.The composite trace element function packet, is made of the raw material of following parts by weight: copper sulphate (copper coin cellulose content
25%) 8 parts, 28 parts of ferrous sulfate (iron content 28%), 23 parts of manganese sulfate (manganese element content 31%), zinc sulfate (zinc member
Cellulose content 34%) 20 parts, 1.6 parts of calcium iodate (content of calcium constituent is 10%), sodium selenite (content of selenium element is 10%)
0.2 part, 0.1 part, 19.1 parts of Kelp Powder of cobalt chloride (content of cobalt element is 10%).The multi-vitamins function packet, under
The raw material of column parts by weight forms: 10 parts of vitamin A (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), dimension
50 parts of raw element E (50 million international units/g), 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, skin of beancurd
5 parts of powder.The immunologic facilitation function packet, is made of the raw material of following parts by weight: 8 parts of semen ziziphi spinosae, 35 parts of Folium sophorae, pine needle meal 20
Part, dried orange peel 3,5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.The compound hair
Yeast-like fungi kind is made of the raw material of following parts by weight: 25 parts of saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/g), aspergillus niger (viable bacteria
Number: 10~2,000,000,000 CFU/g) 35 parts, 28 parts of enterococcus faecalis (viable count: 10~2,000,000,000 CFU/g).
Preparation method, application method are the same as embodiment 2.
Embodiment 4
The beef cattle fermented feed for preparing a kind of promotion lumen fermentation and raising immunity, is by following components in parts by weight
Composition: 5 parts of dregs of beans, 15 parts of corn, 15 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, multi-vitamins function packet 2.5
Part, 18 parts of immunologic facilitation function packet, 4 parts of calcium monohydrogen phosphate, 4 parts of magnesia, 4 parts of potassium chloride, 1.5 parts of composite fermentation strain, molasses 6
Part, 22 parts of pure water.The composite trace element function packet, is made of the raw material of following parts by weight: copper sulphate (copper coin cellulose content
25%) 8 parts, 28 parts of ferrous sulfate (iron content 28%), 23 parts of manganese sulfate (manganese element content 31%), zinc sulfate (zinc member
Cellulose content 34%) 20 parts, 1.6 parts of calcium iodate (content of calcium constituent is 10%), sodium selenite (content of selenium element is 10%)
0.2 part, 0.1 part, 19.1 parts of Kelp Powder of cobalt chloride (content of cobalt element is 10%).The multi-vitamins function packet, under
The raw material of column parts by weight forms: 10 parts of vitamin A (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), dimension
50 parts of raw element E (50 million international units/g), 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, skin of beancurd
5 parts of powder.The immunologic facilitation function packet, is made of the raw material of following parts by weight: 8 parts of semen ziziphi spinosae, 35 parts of Folium sophorae, pine needle meal 20
Part, dried orange peel 3,5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.The compound hair
Yeast-like fungi kind is made of the raw material of following parts by weight: 25 parts of saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/g), aspergillus niger (viable bacteria
Number: 10~2,000,000,000 CFU/g) 35 parts, 28 parts of enterococcus faecalis (viable count: 10~2,000,000,000 CFU/g).
Preparation method, application method are the same as embodiment 2.
Embodiment 5
The beef cattle fermented feed for preparing a kind of promotion lumen fermentation and raising immunity, is by following components in parts by weight
Composition: 5 parts of dregs of beans, 15 parts of corn, 12 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, multi-vitamins function packet 2.5
Part, 18 parts of immunologic facilitation function packet, 5 parts of calcium monohydrogen phosphate, 4 parts of magnesia, 5 parts of potassium chloride, 1.5 parts of composite fermentation strain, molasses 7
Part, 22 parts of pure water.The composite trace element function packet, is made of the raw material of following parts by weight: copper sulphate (copper coin cellulose content
25%) 8 parts, 28 parts of ferrous sulfate (iron content 28%), 23 parts of manganese sulfate (manganese element content 31%), zinc sulfate (zinc member
Cellulose content 34%) 20 parts, 1.6 parts of calcium iodate (content of calcium constituent is 10%), sodium selenite (content of selenium element is 10%)
0.2 part, 0.1 part, 19.1 parts of Kelp Powder of cobalt chloride (content of cobalt element is 10%).The multi-vitamins function packet, under
The raw material of column parts by weight forms: 10 parts of vitamin A (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), dimension
50 parts of raw element E (50 million international units/g), 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, skin of beancurd
5 parts of powder.The immunologic facilitation function packet, is made of the raw material of following parts by weight: 8 parts of semen ziziphi spinosae, 35 parts of Folium sophorae, pine needle meal 20
Part, dried orange peel 3,5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.The compound hair
Yeast-like fungi kind is made of the raw material of following parts by weight: 25 parts of saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/g), aspergillus niger (viable bacteria
Number: 10~2,000,000,000 CFU/g) 35 parts, 28 parts of enterococcus faecalis (viable count: 10~2,000,000,000 CFU/g).
Preparation method, application method are the same as embodiment 2.
6 application experiment of embodiment
In order to verify the present invention to the feeding effect of beef cattle, company technique center was 2 months 2017 26 days in June, 2017
Feeding experiment has been carried out in cattle farm within 18th.Test is designed using single factor test comparative test, and the beef cattle at 120 10-12 monthly ages is random
It is divided into test group and control group, every group includes 4 repetitions, each repetition 6.The additive of control group 1 is the formula of comparative example 1
Feed, the additive of control group 2 are the formula forage of comparative example 2, and the additive of control group 3 is the formula forage of comparative example 3, right
Additive according to group 4 is the formula forage of comparative example 4, and the additive of test group is the formula forage of embodiment 2.
Ration Compound method is same as Example 2.
Test acquisition data include daily gain, disease incidence etc..
Contrast product design is as follows:
Comparative example 1
The meat cattle feed for preparing a kind of promotion lumen fermentation and raising immunity is following components in parts by weight composition
: 5 parts of dregs of beans, 15 parts of corn, 12 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, 2.5 parts of multi-vitamins function packet are exempted from
Epidemic disease, which is strengthened function, wraps 20 parts, and 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia are 4 parts of potassium chloride, 1.5 parts of composite fermentation strain, 6 parts of molasses, pure
22 parts of water purification.The composite trace element function packet, is made of the raw material of following parts by weight: copper sulphate (copper coin cellulose content 25%)
8 parts, 28 parts of ferrous sulfate (iron content 28%), 23 parts of manganese sulfate (manganese element content 31%), zinc sulfate (Zn-ef ficiency content
34%) 20 parts, 1.6 parts of calcium iodate (content of calcium constituent is 10%), 0.2 part of sodium selenite (content of selenium element is 10%),
0.1 part, 19.1 parts of Kelp Powder of cobalt chloride (content of cobalt element is 10%).The multi-vitamins function packet, by following weight
The raw material composition of part: 10 parts of vitamin A (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), vitamin E
50 parts of (50 million international units/g), 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, 5 parts of skin of beancurd powder.
The immunologic facilitation function packet, is made of the raw material of following parts by weight: 8 parts of semen ziziphi spinosae, 35 parts of Folium sophorae, 20 parts of pine needle meal, old
Skin 3,5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.The composite zymocyte
Kind is made of the raw material of following parts by weight: 25 parts of saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/g), aspergillus niger (viable count: 10
~20 hundred million CFU/g) 35 parts, 28 parts of enterococcus faecalis (viable count: 10~2,000,000,000 CFU/g).
It is described it is a kind of promote lumen fermentation and raising immunity meat cattle feed the preparation method comprises the following steps: with 2 phase of embodiment
Than directly each raw material being mixed without fermentation step.
Comparative example 2
The beef cattle fermented feed for preparing a kind of promotion lumen fermentation and raising immunity, is by following components in parts by weight
Composition: 5 parts of dregs of beans, 16.5 parts of corn, 13 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, immunologic facilitation function packet 20
Part, 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia, 4 parts of potassium chloride, 1.5 parts of composite fermentation strain, 6 parts of molasses, 22 parts of pure water.It is described
Composite trace element is made of the raw material of following parts by weight: 8 parts of copper sulphate (copper coin cellulose content 25%), ferrous sulfate (ferro element
Content 28%) 28 parts, 23 parts of manganese sulfate (manganese element content 31%), 20 parts of zinc sulfate (Zn-ef ficiency content 34%), calcium iodate (calcium
The content of element is 10%) 1.6 parts, 0.2 part of 1 sodium selenite (content of selenium element is 10%), the cobalt chloride (content of cobalt element
It is 10%) 0.1 part, 19.1 parts of Kelp Powder.The immunologic facilitation function packet, is made of the raw material of following parts by weight: semen ziziphi spinosae 8
Part, 35 parts of Folium sophorae, 20 parts of pine needle meal, 3 parts of dried orange peel, 5 parts of hawthorn, 3 parts of wrinkled giant hyssop, 3 parts of Radix Angelicae Sinensis, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, green wind
4 parts of rattan, 8 parts of Radix Ophiopogonis.The composite fermentation strain is made of the raw material of following parts by weight: saccharomyces cerevisiae (viable count: 10~2,000,000,000
CFU/g) 25 parts, 35 parts of aspergillus niger (viable count: 10~2,000,000,000 CFU/g), bacillus subtilis (viable count: 10~2,000,000,000 CFU/
G) 28 parts.
Preparation method, application method are the same as embodiment 2.
Comparative example 3
The beef cattle fermented feed for preparing a kind of promotion lumen fermentation and raising immunity, is by following components in parts by weight
Composition: 8 parts of dregs of beans, 22 parts of corn, 22 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, multi-vitamins function packet 2.5
Part, 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia, 4 parts of potassium chloride, 1.5 parts of composite fermentation strain, 6 parts of molasses, 22 parts of pure water.It is described
Composite trace element function packet, is made of the raw material of following parts by weight: 8 parts of copper sulphate (copper coin cellulose content 25%), ferrous sulfate
28 parts of (iron content 28%), 23 parts of manganese sulfate (manganese element content 31%), 20 parts of zinc sulfate (Zn-ef ficiency content 34%), iodine
Sour 1.6 parts of calcium (content of calcium constituent is 10%), 0.2 part of sodium selenite (content of selenium element is 10%), cobalt chloride (cobalt element
Content be 10%) 0.1 part, 19.1 parts of Kelp Powder.The multi-vitamins function packet, is made of the raw material of following parts by weight:
10 parts of vitamin A (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), vitamin E (50 million international units/
G) 50 parts, 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, 5 parts of skin of beancurd powder.The composite zymocyte
Kind is made of the raw material of following parts by weight: 25 parts of saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/g), aspergillus niger (viable count: 10
~20 hundred million CFU/g) 35 parts, 28 parts of enterococcus faecalis (viable count: 10~2,000,000,000 CFU/g).
Preparation method, application method are the same as embodiment 2.
Comparative example 4
The beef cattle fermented feed for preparing a kind of promotion lumen fermentation and raising immunity, is by following components in parts by weight
Composition: 6 parts of dregs of beans, 18 parts of corn, 18 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, multi-vitamins function packet 2.5
Part, 23 parts of immunologic facilitation function packet, 1.5 parts of composite fermentation strain, 6 parts of molasses, 22 parts of pure water.The composite trace element function
It can wrap, be made of the raw material of following parts by weight: 8 parts of copper sulphate (copper coin cellulose content 25%), ferrous sulfate (iron content
28%) 28 parts, 23 parts of manganese sulfate (manganese element content 31%), 20 parts of zinc sulfate (Zn-ef ficiency content 34%), calcium iodate (calcium constituent
Content be 10%) 1.6 parts, (content of cobalt element is for 0.2 part of sodium selenite (content of selenium element is 10%), cobalt chloride
10%) 0.1 part, 19.1 parts of Kelp Powder.The multi-vitamins function packet, is made of: vitamin A the raw material of following parts by weight
10 parts of (50 million international units/g), 3 parts of vitamine D3 (50 million international units/g), 50 parts of vitamin E (50 million international units/g),
10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, 10 parts of vitamin C, 5 parts of skin of beancurd powder.The immunologic facilitation function packet, by
The raw material of following parts by weight forms: 8 parts of semen ziziphi spinosae, 35 parts of Folium sophorae, 20 parts of pine needle meal, dried orange peel 3, and 5 parts of hawthorn, 3 parts of wrinkled giant hyssop, when
Return 3 parts, 6 parts of Radix Glycyrrhizae, 5 parts of Radix Astragali, 4 parts of caulis sinomenii, 8 parts of Radix Ophiopogonis.The composite fermentation strain by following parts by weight raw material group
At: 25 parts of saccharomyces cerevisiae (viable count: 10~2,000,000,000 CFU/g), 35 parts of aspergillus niger (viable count: 10~2,000,000,000 CFU/g), excrement intestines ball
28 parts of bacterium (viable count: 10~2,000,000,000 CFU/g).
Preparation method, application method are the same as embodiment 2.
Test group and control group fed mode are to be freely eaten, and experimental period is 2 months.
Test result is as shown in table 1, the results showed that
Table 3 adds the influence of the embodiment of the present invention and comparative example feed to beef cattle daily gain and disease incidence
| Project | Control group 1 | Control group 2 | Control group 3 | Control group 4 | Test group |
| Feed intake (kg/d) | 7.16 | 7.38 | 7.52 | 7.63 | 7.82 |
| Daily gain (kg/d) | 0.81 | 0.89 | 0.92 | 0.89 | 1.32 |
| Disease incidence (%) | 2.21 | 1.84 | 1.59 | 1.73 | 0.43 |
Note: with natural air drying poidometer.
It can be obtained by the data of table 3, compared to the technical solution of control group, cattle feed production performance of the invention is increasing day by day
Obvious improvement is all had in terms of weight, elimination of nitrogen amount, test group figure is preferable, shows that lumen fermentation is good.This illustrates to match
The selection of square raw material components, dosage and compound strain is all very crucial, and the present invention is drained for beef cattle growth and nitrogen decrement
It is required that the formula and consumption proportion of design are very scientific and reasonable, and cost is relatively low.
The experiment of 7 lumen fermentation of embodiment
1, experimental animal and feeding management
Selecting 8 healthy adult fistula beef cattles is experimental animal, is randomly divided into 4 groups, every morning 8:00 and 5:00 in afternoon
Feeding is freely eaten, sufficient free water.
2, daily ration and experimental design are tested
Test is divided into 4 processing, and 2 beef cattles of each processing are respectively as follows:
Control 1: additive is the formula forage of comparative example 1；
Control 2: additive is the formula forage of comparative example 2；
Control 3: additive is the formula forage of comparative example 4；
Test group: additive is the formula forage of embodiment 2.
Ration Compound method is same as Example 2.
Experimental period is 30 days.
3, the measurement of beef cattle Rumen Fermentation
2h after last day the 30th day of test, morning feeding acquires 40mL rumen fluid from the rumen fistula of every beef cattle,
By 4 layers of filtered through gauze, first measurement pH, then keeps sample and measure VFA, NH3-N。
(1) pH is measured: quickly measuring rumen fluid ph with electronics pH meter after having acquired rumen fluid.
(2) VFA is measured: taking 5ml to filter rumen fluid, the metaphosphoric acid buffer solution of 1ml30% (w/v) is added, then uses
The concentration of gas chromatography measurement volatile fatty acid (VFA) concentration and general volatile fatty acid (TVFA).
(3)NH3- N measurement: 5mL filters rumen fluid and 1mL 1% (v/v) H is added2SO4Solution.Using phenol-sodium hypochlorite
NH in colorimetric method for determining rumen fluid3The concentration of-N.
(4) measurement of oxidation-reduction potential (Eh): using the Eh of ORP potential measurement instrument measurement rumen fluid.
4, diet dry matter and crude protein are measured
It selects aperture that the Nylon Bag of 8 × 10cm is made for the nylon cloth of 200 mesh, weighs test specimen 8g respectively and be put into Buddhist nun
It in imperial bag, is tied using rubber band, then Nylon Bag is fixed on to one end of the medium-soft nylon tube of a root long 20cm, before morning raises
1h is put into beef cattle cud, incubation time 36h, continuous sampling, setting-out, takes out Nylon Bag at the appointed time, and with enough
Water cleaned, until clarification of water.Then by the Nylon Bag cleaned up, drying to constant weight in 70 DEG C.It will be in Nylon Bag
Degradation after sample pour out after, clean, same 70 DEG C drying to constant weight, the dry matter of measurement degradation front and back feedstuff and thick
The degradation rate of albumen.
5, result
Influence result of 5.1 each sample to lumen fermentation
It is shown in Table 4.
Influence result of 4 each sample of table to lumen fermentation
| Project | Control 1 | Control 2 | Control 3 | Test group |
| PH value | 5.8 | 6.4 | 6.0 | 6.5 |
| NH3-N(mmol/L) | 15.5 | 13.7 | 12.9 | 10.4 |
| TVFA(mmol/L) | 69.4 | 79.5 | 77.5 | 82.3 |
| ORP(mv) | 350.1 | 364.2 | 254.3 | 356.0 |
| Acetic acid (%) | 53.6 | 52.7 | 51.4 | 52.3 |
| Propionic acid (%) | 23.1 | 22.9 | 23.4 | 23.0 |
| Butyric acid (%) | 13.2 | 14.5 | 13.0 | 14.1 |
| Isobutyric acid (%) | 0.6 | 0.7 | 0.5 | 0.6 |
| Valeric acid (%) | 5.3 | 5.6 | 6.1 | 6.2 |
| Isovaleric acid (%) | 4.2 | 3.6 | 5.6 | 3.8 |
It can be obtained by table 4, the feed of test group can effectively control the pH of rumen fluid 6.5, and buffer capacity is good, Neng Goubao
Demonstrate,prove the health steady in a long-term of lumen fermentation；Cud internal oxidition reduction potential is 356mv or so, is conducive in beef cattle cud detesting property partially
Flora is inhabited；Ammonia in cud is excessive, ammonia can be made to be rapidly absorbed into blood, is easy to make urea nitrogen in blood how high, because
This needs to reduce ammonia burst size, and test group is compared to control group NH3- N is significantly reduced, and illustrates that the feed of test group can be effective
Reduce the burst size of cud ammonia；Effect VFA most basic in ruminant body is just to provide the energy, as known from Table 2, different
Influence of the daily ration sample to each VFA concentration in cud is variant, and compared with the control group, the general volatile fatty acid of test group is aobvious
It writes and improves, the Fermentation Function of energy Effective Regulation beef cattle cud improves the bioactivity of volatile fatty acid and microorganism.To sum up,
This illustrates that the selection of the type of formula material component and dosage and compound strain is all very crucial, and the present invention is improving beef cattle
In lumen fermentation performance, the formula and consumption proportion of design are very scientific and reasonable, and cost is relatively low.
Influence result of 5.2 each sample to diet dry matter and crude protein degradation rate
Degradation rate the result is shown in Figure 1 and Fig. 2 of the different samples to feedstuff dry matter and crude protein.
By Fig. 1~2 it is found that compared with the control group, test group can significantly improve the degradation of diet dry matter and crude protein
Rate, it can be deduced that test group makes the cud of beef cattle have good fermenting property, can improve the degradation rate of diet dry matter
The degradation rate of crude protein can be also improved simultaneously.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (9)
1. a kind of beef cattle fermented feed for promoting lumen fermentation and improving immunity, characterized in that be by following weight parts
Component is made:
3-8 parts of dregs of beans, 8-25 parts of corn, 10-20 parts of fruits and vegetables slag, 0.5-5 parts of composite trace element function packet, multi-vitamins function
It can wrap 1-4 parts, 15-25 parts of immunologic facilitation function packet, 3-6 parts of calcium monohydrogen phosphate, 4-7 parts of magnesia, 2-5 parts of potassium chloride, compound hair
1-3 parts of yeast-like fungi kind, 5-10 parts of molasses, 20-30 parts of water；
Wherein, the immunologic facilitation function packet is made of the raw material of following parts by weight: 5-15 parts of semen ziziphi spinosae, 15-40 parts of Folium sophorae,
10-25 parts of pine needle meal, 1-5 parts of dried orange peel, 1-5 parts of hawthorn, 2.5-10 parts of wrinkled giant hyssop, 2.5-10 parts of Radix Angelicae Sinensis, 2.5-10 parts of Radix Glycyrrhizae, Radix Astragali
2.5-10 parts, 1-5 parts of caulis sinomenii, Radix Ophiopogonis 2.5-10 part；
The composite fermentation strain is made of the raw material of following parts by weight: 15-30 parts of saccharomyces cerevisiae, 20-40 parts of aspergillus niger, excrement
20-35 parts of enterococcus.
2. feed as described in claim 1, characterized in that be made of following components in parts by weight:
5 parts of dregs of beans, 15 parts of corn, 12 parts of fruits and vegetables slag, 3 parts of composite trace element function packet, 2.5 parts of vitamin function packet are immunized
It strengthens function and wraps 20 parts, 4 parts of calcium monohydrogen phosphate, 5 parts of magnesia are 4 parts of potassium chloride, 1.5 parts of composite fermentation strain, 6 parts of molasses, pure
22 parts of water.
3. feed as described in claim 1, characterized in that the composite trace element function packet is by the original of following parts by weight
Material composition: 5-10 parts of copper sulphate, 20-40 parts of ferrous sulfate, 15-30 parts of manganese sulfate, 15-25 parts of zinc sulfate, 1-3 parts of calcium iodate,
0.1-0.3 parts of sodium selenite, 0.05-0.2 parts of cobalt chloride, 18-22 parts of Kelp Powder.
4. feed as claimed in claim 3, characterized in that the composite trace element function packet is by the original of following parts by weight
Material composition:
8 parts of copper sulphate, 28 parts of ferrous sulfate, 23 parts of manganese sulfate, 20 parts of zinc sulfate, 1.6 parts of calcium iodate, 0.2 part of sodium selenite, chlorine
Change 0.1 part of cobalt, 19.1 parts of Kelp Powder.
5. feed as described in claim 1, characterized in that the vitamin function packet is made of the raw material of following parts by weight:
10 parts of vitamin A, 2-5 parts of vitamine D3,40-60 parts of vitamin E, 8-10 parts of niacinamide, 5-8 parts of biotin, antioxidant
4-8 parts, 8-15 parts of vitamin C, 3-8 parts of skin of beancurd powder.
6. feed as claimed in claim 5, characterized in that the vitamin function packet is the raw material group by following parts by weight
At: 10 parts of vitamin A, 3 parts of vitamine D3,50 parts of vitamin E, 10 parts of niacinamide, 7 parts of biotin, 5 parts of antioxidant, dimension
Raw 10 parts, 5 parts of skin of beancurd powder of element C.
7. the system of the beef cattle fermented feed according to any one of claims 1 to 6 for promoting lumen fermentation and improving immunity
Preparation Method, characterized in that method includes the following steps:
(1) other components by the feed in addition to composite fermentation strain stir evenly to obtain feedstuff in parts by weight；
(2) the composite fermentation strain bacterium solution after activation is seeded in the feedstuff of step (1) ferment curing to obtain and is promoted
Lumen fermentation and the beef cattle fermented feed for improving immunity.
8. fermented feed according to any one of claims 1 to 6 promotes beef cattle lumen fermentation and improves immunity to raise in preparation
Application in material.
9. a kind of method for promoting beef cattle lumen fermentation and improving immunity, it is characterized in that: this method includes that beef cattle eats right
It is required that the step of promotion lumen fermentation described in any one of 1~6 and beef cattle fermented feed of raising immunity；
Specifically, by natural wind dry sample restatement, additive amount is the 1% of beef cattle perfect compound feed before beef cattle weight 150kg
Additional addition；In beef cattle weight 150kg to delivering for sale, by natural wind dry sample restatement, additive amount is beef cattle perfect compound feed
2% additional addition；First the feed and beef cattle perfect compound feed are sufficiently mixed, then used after being sufficiently mixed with roughage.
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| CN110012975A (en) * | 2019-05-11 | 2019-07-16 | 辽宁九州生物科技有限公司 | A kind of preparation method of beef cattle concentration fermentation material |
| CN110859245A (en) * | 2019-12-18 | 2020-03-06 | 安徽五粮泰生物工程股份有限公司 | Preparation method of functional feed for preventing and treating ruminal acidosis of ruminants |
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