The method of erythropsis kwangsiensis four seasons rapid seedling cultivation
Technical field
The invention belongs to plant asexual multiplication technology field, be specifically related to a kind of method of erythropsis kwangsiensis four seasons rapid seedling cultivation.
Background technology
Erythropsis kwangsiensis (Ery th rop siskwang siensis (Hsue) Hsue); Sterculiaceae (Sterculiaceae) deciduous tree, the distinctive national 2 grades of protected trees in Guangxi, originate in Jingxi, upper think of; carry out current resources situation investigation in 5~June in 2005, find altogether 12 strains.The erythropsis kwangsiensis of finding is all distributed in the bottom or gentle slope of Limestone Mountain, and in Shangsi County, former area, but can not find trace.When erythropsis kwangsiensis has arrived necessary rescue protection.
Erythropsis kwangsiensis, the deciduous species in summer that occurring in nature is rare, after fallen leaves, full tree is full of safflower, very beautiful.Having very high ornamental value, is the good merchantable brand of afforestation and street tree.Take protection to develop, can not only change the destiny that it is exterminated, and can obtain good economy, ecology and social benefit.
At present, erythropsis kwangsiensis is mainly bred by seed, but learn that from " erythropsis kwangsiensis is collected seed and seedling growing process " literary composition that < < Guangxi forestry > > 01 phase in 2008 is delivered by Huang Hanjing, Lu Shinian, Wei Lanying etc. erythropsis kwangsiensis is because the kind skin of seed is thinner, volatile moisture content, the contracting of kind rhicnosis and variable color, reduce or lose germinating capacity, this is that it becomes one of reason of endangered species.Moreover, at present erythropsis kwangsiensis also just only remaining so several strains in the wild, the seed of also just can gathering a time every year, that has just limited to so to a great extent erythropsis kwangsiensis numerously prolongs growth.
" tissue of erythropsis kwangsiensis is cultivated and plant regeneration " literary composition of delivering from the 46th the 12nd phase of volume of < < Plant Physiology Communications > > in December, 2010, learned paying of Guangxi Academy Of Sciences Institute of Zoology pass bright, that Huang Ningzhen, Luo Wenhua etc. utilize test tube tissue culture technology to breed erythropsis kwangsiensis nursery stock is successful.But well-known, it is very huge that the infrastructure of tissue culture technology drops into, and specification requirement is very professional.Tissue culture technology operation is very complicated, very loaded down with trivial details, thus the erythropsis kwangsiensis nursery stock of producing by test tube tissue culture technology not only the cycle long, and integrated cost is high.
Summary of the invention
The object of the present invention is to provide the asexual four seasons fast breeding technique of the non-test tube of a kind of erythropsis kwangsiensis, it has overcome erythropsis kwangsiensis seminal propagation and has brought to obtain limitation, has overcome again that test tube tissue culture technology infrastructure has high input, specification requirement specialty, complex procedures and the shortcoming such as loaded down with trivial details.Provide that a kind of technique is simple, infrastructure investment is few and can realize the erythropsis kwangsiensis quick-breeding method of anniversary, batch production, automation.
The present invention is achieved through the following technical solutions:
1, the seedbed construction method of erythropsis kwangsiensis four seasons rapid seedling cultivation:
(1) foundation in substrate culture seedbed: selective light is according to sufficient, seedbed is built on the good ground of draining, seedbed specification is built high furrow by long 5m, wide 1.2~1.5m, on furrow limit, with brick, build the edge of bed of high 15~20cm, between brick and brick, slightly stay gap in order to the discharge of water, between seedbed, stay the aisle of 30~50cm in order to operation; Matrix is selected perlite, and the thickness that matrix spreads is consistent with edge of bed height; The effect in matrix seedbed is to fix fiery paulownia cutting by matrix, and provides the carrier of moisturizing, ventilative and gnotobasis for fiery paulownia cutting.
(2) foundation in air culture seedbed: the support of making a long 5m, wide 1.2~1.5m, liftoff 30cm with timber or steel, on support, lay specification is the plastic wire of 0.5~1 centimeter of mesh, the little shed that to build a specification be long 5.5m, wide 1.3~1.6m, high 120cm again covers support, little shed covering plastic foil and shading screen; The effect in air culture seedbed is fiery paulownia cutting is directly emitted on rack and does not need by matrix, the cutting of fire paulownia is in the environment without matrix, acquisition carbon dioxide and oxygen that can be sufficient, be conducive to otch and form callus, solved when day hot the cutting of fire paulownia and be prone to infection of incisional wound and to rotting, wait and cause a dead difficult problem.
2, the seedbed water system method for building up of erythropsis kwangsiensis four seasons rapid seedling cultivation:
(1) micro-nozzle road on frame above center, seedbed, micro-nozzle road is 60~70cm far from bed surface, on pipeline, at interval of 50cm, an atomizer is installed, the direction that shower nozzle is installed is vertically upward.
(2) micro-nozzle road is connecting water pump; During work, be to be supplied water by water pump, and spray and form water atomization shape from atomizer by pipeline under the pressure that water produces when water pump supplies water, at air, fill the air, thereby reach needed humidity requirement in seedling raising environment.
(3) water pump is to be controlled by the time relay; The effect of the time relay is when to spray water for setting by people, and spray is water how long, and the time relay will be that the parameter that sets is controlled water pump and sprayed water according to people.
(4) at the head of a bed in seedbed, establish tank, water pump is placed in tank.
3, the collection of erythropsis kwangsiensis cutting and preparation method:
(1) collection of fiery paulownia cutting: select the tree body of vigorous, the disease-free and insect pest of growth as female parent, such To body material physiological function and metabolism are vigorous, are conducive to the healing of otch and the formation of root system; Cutting is chosen in morning, dusk and such period of overcast and rainy whole day and gathers.
(2) making of fiery paulownia cutting: adopt sharp pruning shear that fiery paulownia cutting is cut into leaf two buds or branch sections more than two buds, cutting at least will be stayed a blade.
(3) according to the magnitude classification of fiery paulownia cutting, lash standby.
4, fast the selecting and compound method of root-inducing powder when numerous of erythropsis kwangsiensis:
(1) selecting of root-inducing powder: fiery paulownia cutting, in fast numerous link, selects taking root liquid that ABT1 root-inducing powder is made to carry out accelerating after otch processing that callus forms and the generation of the former base of root, and the amount of taking root in later stage also has obvious growth.
(2) the ABT1 root-inducing powder of 1 gram was first diluted in the preparation of liquid: ABT1 root-inducing powder difficulty is dissolved in water before being mixed with taking root liquid with the medicinal alcohol that 300 milliliters of content are 75%, then added clear water and be dissolved to 10000 milliliters and become taking root liquids.
5, the method for taking root liquid is soaked in erythropsis kwangsiensis cutting:
(1) by the fiery paulownia cutting lashing with base portion down and by size cutting classify, neat concentrated being placed in plastic basin, pours in basin the taking root liquid preparing to flooding fiery paulownia cutting base portion into respectively.
(2) diameter is that fiery paulownia cutting below 5cm is soaked taking root liquid 2~3 hours, and diameter is that fiery paulownia cutting more than 5cm is soaked taking root liquid 3~5 hours.
6, erythropsis kwangsiensis cutting is processed time and method:
(1) fiery paulownia cutting, 1 annual~June, adopts substrate culture 10~December.
(2) fiery paulownia cutting adopts air culture 7 annual~September.
(3), when fiery paulownia cutting is inserted in substrate culture seedbed, in good order being inserted in matrix and with the blade of fiery paulownia cutting heavily not build and be as the criterion, 1m
2seedbed general insert 200 fiery paulownia cuttings.
(4), when fiery paulownia cutting is put into air culture seedbed, in good order being placed in to net bed upper, and heavily not building and being as the criterion, 1m with fiery paulownia cutting
2seedbed general settle 250 fiery paulownia cuttings.
7, erythropsis kwangsiensis cutting is in the management method in substrate culture seedbed:
(1) water spray management: once fiery paulownia cutting is left after female parent, the moisture supply of cutting is also just disconnected, so fiery paulownia cutting is inserted into after medium of seedling bed, the spraying that is about to begin is moistening at any time to guarantee branch body and the blade of fiery paulownia cutting; Before the callus of fire paulownia cutting forms, the mist frequency of spraying is to spray water 10~15 seconds for every 1:30 hour, the frequency of spraying after the generation of the former base of root of fiery paulownia cutting changes 3 hours into and sprays water 8~10 seconds, and the longer rear spraying frequency of coring of fiery paulownia cutting changes 5 hours into and sprays water 8~10 seconds; To evening or overcast and rainyly can cut off spraying system.
(2) foliar application: after the generation of the former base of root of fiery paulownia cutting, spraying concentration is 0.2%~0.3% biphosphate potassium foliage fertilizer, sprays once for every 7 days, until fiery paulownia seedling transplanting.
(3) nursery bed disinfection: fiery paulownia cutting is inserted into behind seedbed, wants every 7 days that at candlelight and stops many bacterium liquid thimerosal that spraying system is 800 times to seedbed spray concentration to carry out disinfection, until fiery paulownia seedling transplanting.
8, erythropsis kwangsiensis cutting is in the management method in air culture seedbed:
(1) water spray management: once fiery paulownia cutting is left after female parent, the moisture supply of cutting is also just disconnected, so fiery paulownia cutting is placed into behind air culture seedbed, the spraying that is about to begin, guarantees that branch body and the blade of fiery paulownia cutting is moistening at any time; Before the callus of fire paulownia cutting forms, the mist frequency of spraying is to spray water 10~15 seconds for every 30 minutes, the frequency of spraying after the generation of the former base of root of fiery paulownia cutting changes 1:30 hour into and sprays water 8~10 seconds, and the longer rear spraying frequency of coring of fiery paulownia cutting changes 3 hours into and sprays water 8~10 seconds; To evening or overcast and rainyly can cut off spraying system.
(2) foliar application: after the generation of the former base of root of fiery paulownia cutting, spraying concentration is 0.2%~0.3% biphosphate potassium foliage fertilizer, sprays once for every 7 days, until fiery paulownia seedling transplanting.
(3) nursery bed disinfection: air culture is just carried out in fiery paulownia cutting when high temperature season, and the space in air culture in this time seedbed is also because hot and humid and grow a lot of microorganisms, these microbes cause death to rotting to the infection of incisional wound of fiery paulownia cutting, so the many bacterium liquid thimerosal that is 800 times to air culture seedbed spray concentration after wanting that at candlelight for 2 days to stop spraying system carrying out disinfection, until fiery paulownia seedling transplanting.
9, trasplanting method after erythropsis kwangsiensis rooting of cuttings:
When the base portion of fiery paulownia cutting grows after square root, just can be fiery paulownia transplantation of seedlings to field production.
Compared with prior art, the present invention both had the following advantages:
1, the present invention has overcome the limitation of erythropsis kwangsiensis seminal propagation;
2, the whole technological process of the present invention is simple, infrastructure investment is few;
3, the present invention can realize anniversary, batch production, automation and breed erythropsis kwangsiensis seedling;
4, the erythropsis kwangsiensis that uses the present invention to breed is with short production cycle, integrated cost is low;
5, the invention belongs to vegetative propagation, can keep maternal good characteristic, reproduction speed fast.
6, the present invention's amount reproduction erythropsis kwangsiensis in a short time, and seedling root is flourishing, robust growth.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
1, the seedbed construction method of erythropsis kwangsiensis four seasons rapid seedling cultivation:
(1) foundation in substrate culture seedbed: selective light is according to sufficient, seedbed is built on the good ground of draining, seedbed specification is built high furrow by long 5m, wide 1.2~1.5m, on furrow limit, with brick, build the edge of bed of high 15~20cm, between brick and brick, slightly stay gap in order to the discharge of water, between seedbed, stay the aisle of 30~50cm in order to operation; Matrix is selected perlite, and the thickness that matrix spreads is consistent with edge of bed height; The effect in matrix seedbed is to fix fiery paulownia cutting by matrix, and provides the carrier of moisturizing, ventilative and gnotobasis for fiery paulownia cutting.
(2) foundation in air culture seedbed: the support of making a long 5m, wide 1.2~1.5m, liftoff 30cm with timber or steel, on support, lay specification is the plastic wire of 0.5~1 centimeter of mesh, the little shed that to build a specification be long 5.5m, wide 1.3~1.6m, high 120cm again covers support, little shed covering plastic foil and shading screen; The effect in air culture seedbed is fiery paulownia cutting is directly emitted on rack and does not need by matrix, the cutting of fire paulownia is in the environment without matrix, acquisition carbon dioxide and oxygen that can be sufficient, be conducive to otch and form callus, solved when day hot the cutting of fire paulownia and be prone to infection of incisional wound and to rotting, wait and cause a dead difficult problem.
2, the seedbed water system method for building up of erythropsis kwangsiensis four seasons rapid seedling cultivation:
(1) micro-nozzle road on frame above center, seedbed, micro-nozzle road is 60~70cm far from bed surface, on pipeline, at interval of 50cm, an atomizer is installed, the direction that shower nozzle is installed is vertically upward.
(2) micro-nozzle road is connecting water pump; During work, be to be supplied water by water pump, and spray and form water atomization shape from atomizer by pipeline under the pressure that water produces when water pump supplies water, at air, fill the air, thereby reach needed humidity requirement in seedling raising environment.
(3) water pump is to be controlled by the time relay, and the effect of the time relay is when to spray water for setting by people, and spray is water how long, and the time relay will be that the parameter that sets is controlled water pump and sprayed water according to people.
(4) at the head of a bed in seedbed, establish tank, water pump is placed in tank.
3, the collection of erythropsis kwangsiensis cutting and preparation method:
(1) collection of fiery paulownia cutting: select the tree body of vigorous, the disease-free and insect pest of growth as female parent, such To body material physiological function and metabolism are vigorous, are conducive to the healing of otch and the formation of root system; Cutting is chosen in morning, dusk and such period of overcast and rainy whole day and gathers.
(2) making of fiery paulownia cutting: adopt sharp pruning shear that fiery paulownia cutting is cut into leaf two buds or branch sections more than two buds, cutting at least will be stayed a blade.
(3) according to the magnitude classification of fiery paulownia cutting, lash standby.
4, fast the selecting and compound method of root-inducing powder when numerous of erythropsis kwangsiensis:
(1) selecting of root-inducing powder: fiery paulownia cutting, in fast numerous link, selects taking root liquid that ABT1 root-inducing powder is made to carry out accelerating after otch processing that callus forms and the generation of the former base of root, and the amount of taking root in later stage also has obvious growth.
(2) the ABT1 root-inducing powder of 1 gram was first diluted in the preparation of liquid: ABT1 root-inducing powder difficulty is dissolved in water before being mixed with taking root liquid with the medicinal alcohol that 300 milliliters of content are 75%, then added clear water and be dissolved to 10000 milliliters and become taking root liquids.
5, the method for taking root liquid is soaked in erythropsis kwangsiensis cutting:
(1) by the fiery paulownia cutting lashing with base portion down and by size cutting classify, neat concentrated being placed in plastic basin, pours in basin the taking root liquid preparing to flooding fiery paulownia cutting base portion into respectively.
(2) diameter is that fiery paulownia cutting below 5cm is soaked taking root liquid 2~3 hours, and diameter is that fiery paulownia cutting more than 5cm is soaked taking root liquid 3~5 hours.
6, erythropsis kwangsiensis cutting is processed time and method:
(1) fiery paulownia cutting, 1 annual~June, adopts substrate culture 10~December.
(2) fiery paulownia cutting adopts air culture 7 annual~September.
(3), when fiery paulownia cutting is inserted in substrate culture seedbed, in good order being inserted in matrix and with the blade of fiery paulownia cutting heavily not build and be as the criterion, 1m
2seedbed general insert 200 fiery paulownia cuttings.
(4), when fiery paulownia cutting is put into air culture seedbed, in good order being placed in to net bed upper, and heavily not building and being as the criterion, 1m with fiery paulownia cutting
2seedbed general settle 250 fiery paulownia cuttings.
7, erythropsis kwangsiensis cutting is in the management method in substrate culture seedbed:
(1) water spray management: once fiery paulownia cutting is left after female parent, the moisture supply of cutting is also just disconnected, so fiery paulownia cutting is inserted into after medium of seedling bed, the spraying that is about to begin is moistening at any time to guarantee branch body and the blade of fiery paulownia cutting; Before the callus of fire paulownia cutting forms, the mist frequency of spraying is to spray water 10~15 seconds for every 1:30 hour, the frequency of spraying after the generation of the former base of root of fiery paulownia cutting changes 3 hours into and sprays water 8~10 seconds, and the longer rear spraying frequency of coring of fiery paulownia cutting changes 5 hours into and sprays water 8~10 seconds; To evening or overcast and rainyly can cut off spraying system.
(2) foliar application: after the generation of the former base of root of fiery paulownia cutting, spraying concentration is 0.2%~0.3% biphosphate potassium foliage fertilizer, sprays once for every 7 days, until fiery paulownia seedling transplanting.
(3) nursery bed disinfection: fiery paulownia cutting is inserted into behind seedbed, wants every 7 days that at candlelight and stops many bacterium liquid thimerosal that spraying system is 800 times to seedbed spray concentration to carry out disinfection, until fiery paulownia seedling transplanting.
8, erythropsis kwangsiensis cutting is in the management method in air culture seedbed:
(1) water spray management: once fiery paulownia cutting is left after female parent, the moisture supply of cutting is also just disconnected, so fiery paulownia cutting is placed into behind air culture seedbed, the spraying that is about to begin, guarantees that branch body and the blade of fiery paulownia cutting is moistening at any time; Before the callus of fire paulownia cutting forms, the mist frequency of spraying is to spray water 10~15 seconds for every 30 minutes, the frequency of spraying after the generation of the former base of root of fiery paulownia cutting changes 1:30 hour into and sprays water 8~10 seconds, and the longer rear spraying frequency of coring of fiery paulownia cutting changes 3 hours into and sprays water 8~10 seconds; To evening or overcast and rainyly can cut off spraying system.
(2) foliar application: after the generation of the former base of root of fiery paulownia cutting, spraying concentration is 0.2%~0.3% biphosphate potassium foliage fertilizer, sprays once for every 7 days, until fiery paulownia seedling transplanting.
(3) nursery bed disinfection: air culture is just carried out in fiery paulownia cutting when high temperature season, and the space in air culture in this time seedbed is also because hot and humid and grow a lot of microorganisms, these microbes cause death to rotting to the infection of incisional wound of fiery paulownia cutting, so the many bacterium liquid thimerosal that is 800 times to air culture seedbed spray concentration after wanting that at candlelight for 2 days to stop spraying system carrying out disinfection, until fiery paulownia seedling transplanting.
9, trasplanting method after erythropsis kwangsiensis rooting of cuttings:
When the base portion of fiery paulownia cutting grows after square root, just can be fiery paulownia transplantation of seedlings to field production.