CN102951958A - Anti-disease greenhouse vegetable fertilizer and preparation technology thereof - Google Patents
Anti-disease greenhouse vegetable fertilizer and preparation technology thereof Download PDFInfo
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- CN102951958A CN102951958A CN2011102505308A CN201110250530A CN102951958A CN 102951958 A CN102951958 A CN 102951958A CN 2011102505308 A CN2011102505308 A CN 2011102505308A CN 201110250530 A CN201110250530 A CN 201110250530A CN 102951958 A CN102951958 A CN 102951958A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to an anti-disease greenhouse vegetable fertilizer which is characterized by comprising bacterial enzyme with effective viable count of (2.0-3.0)*10<9>CFU/9, shale, straw powder, dry chicken manure, bone meal, fish meal, soybean meal, borax, magnesium sulfate, ferrous sulfate, potassium dihydrogen phosphate and manganese sulfate. The greenhouse vegetable fertilizer consists of bacterial enzyme, organic matters and microelements and can comprehensively meet the growth and development needs of crops and increase the output by over 15%; the fertilization cost is reduced, and the output-input ratio is increased by over 18%; and moreover, the greenhouse vegetable fertilizer enhances the ability of resisting pest and disease damage of crops, and can perfectly prevent gray mold, leaf mold, anthracnose, early blight, late blight, leaf spot disease and the like.
Description
Technical field
The present invention relates to fertilizer, specifically, relate to booth vegetable fertilizer and preparation technology thereof.
Background technology
The characteristics of vegetables in greenhouse booth are that high temperature, high humidity, airiness are slow, and the Fast Growth that is conducive to vegetables under this environment also is the hotbed that the disease worm grows in a large number simultaneously.The common disease of vegetables in greenhouse booth has: gray mold, leaf mold, anthrax, early blight, late blight, leaf spot etc., the speed of growth that these diseases not only affect vegetables more affects the quality of vegetables, not only take effect slowly in a large amount of various sterilant that spray of having fallen ill again, more affect the quality of vegetables.
Existing booth vegetable fertilizer is fresh chicken manure and composite fertilizer, and these fertilizer nutrient content are higher, but fertilizer efficiency is short, fertilizer nutrient easily runs off, plant recovery of nutrient is low, and uses spoiled soil crumb structure and Ecological Distribution of Soil Microorganisms balance in a large number, reduces the soil water-reataining fertilizer-keeping function, cause vicious cycle, contaminate environment, the fertilising cost rises year by year, and ratio for input and output is lower, disease occurs frequent, and cost accounting is high.
Summary of the invention
The object of the invention is to, in view of the foregoing defects the prior art has, provide a kind of booth vegetable be used to preventing and treating disease with fertile, formed by enzymatic microorganism, organic matter and trace element; The demand of growing of crop can be satisfied comprehensively, output can be improved more than 15%; Reduce the fertilising cost, ratio for input and output increases more than 18%; Improve crop disease-resistant insect pest ability, can well prevent gray mold, leaf mold, anthrax, early blight, late blight, leaf spot etc.
A kind of booth vegetable be used to preventing and treating disease provided by the invention is with fertile, and its improvements are to comprise that living bacteria count is 2.0-3.0 * 10
9The enzymatic microorganism of CFU/g, shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate.
The provided by the invention first preferred booth vegetable that is used for preventing and treating disease is with fertile, and described enzymatic microorganism is bacterium, yeast, fungi and actinomycetes.
The provided by the invention second preferred booth vegetable that is used for preventing and treating disease is with fertile, and the weight part of described shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 300-600 part, straw powder 100-300 part, dried poultrymanure 100-350 part, fish meal 50-100 part, bone meal 10-50 part, dregs of beans 50-100 part, borax 300-800 part, sal epsom 300-900 part, ferrous sulfate 550-1000 part, potassium primary phosphate 50-15 part, manganous sulfate 300-1000 part.
The provided by the invention the 3rd preferred booth vegetable that is used for preventing and treating disease is with fertile, and the weight part of described shale, straw powder, dried poultrymanure, bone meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 352-425 part, straw powder 130-178 part, dried poultrymanure 220-300 part, fish meal 70-90 part, bone meal 30-40 part, dregs of beans 70-80 part, borax 500-750 part, sal epsom 400-810 part, ferrous sulfate 600-900 part, potassium primary phosphate 80-100 part, manganous sulfate 500-800 part.
The provided by the invention the 4th preferred booth vegetable that is used for preventing and treating disease is with fertile, and described bacterium is one or more the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and the pseudomonas; Described yeast is Pichia yeast and/or intends the ball yeast; Described fungi is trichoderma harziarum and/or viride; Described actinomycetes are Streptomycin sulphate.
Bacillus licheniformis provided by the invention, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The fertile preparation technology of booth vegetable be used to preventing and treating disease provided by the invention may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, press (1-2): (1.5-3): (0.6-2): volume ratio (0.5-2) is made composite flora;
2) spawn culture
A, culture dish is placed in the 22-28 ℃ of incubator to cultivate chose bacterium in 3-7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 2-4 time, was raised to 42-45 ℃ of beginning turning through 24-48 hour temperature, once a day; Pile warm 55-60 ℃, turning 5-8 time;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
The provided by the invention first preferred fertile preparation technology of booth vegetable who is used for preventing and treating disease may further comprise the steps:
After b, raw material were piled up well, every day, thermometric twice, and be raised to 42-45 ℃ beginning turning through 72 hours temperature winter, once a day; Pile warm 55-60 ℃, at once turning, turning 5-7 time.
The sign that fermentation is finished: the heap temperature drop is low, and material is loose, without the former stink of material, wine flavour is arranged slightly, produces white hypha in the heap.
Weight part is: gram, kilogram, ton etc.
In order to replenish the required material of vegetables, advance is that power is good, is used in vegetables usefulness, and by the growth of bacterium, breeding can be conducive to the material of vegetable growth and disease and insect resistance in a large number
Living bacteria count is 2.0-3.0 * 10
9The enzymatic microorganism of CFU/g reaches this viable count, just can well bring into play the effect of bacterium.
By the last data that a large amount of experiments is determined, in the regular hour, reach an optimum value, when excess Temperature, just fermentation is exerted an influence, so want turning to lower the temperature.
Compared with prior art, a kind of booth vegetable special fertilizer be used to preventing and treating disease provided by the invention and preparation method thereof has the following advantages:
1, Promoting plant growth mainly be by wherein contained function yeast in the plant root field planting, live and play a role.This effect is the substance that produces Promoting plant growth by function yeast;
2, improve the plant disease-proof resistance against diseases, namely biological control effect, the mode that this class bacterial classification acts on pathogenic bacteria mainly contains three kinds: a. antagonistic action; Produce antagonistic substance and directly suppress the pathogenic bacteria growth; B. Competition suppresses the pathogenic bacteria growth from aspects such as dietetic alimentation utilizations, and this growth speed is very fast generally speaking.C. parasitization take pathogenic bacteria as parasitic, directly colonizes in pathogenic bacteria and causes cause of disease dead with it;
3, can improve output more than 15%.Reduce the fertilising cost, ratio for input and output increases more than 18%; Improve crop disease-resistant insect pest ability, can well prevent gray mold, leaf mold, anthrax, early blight, late blight, leaf spot etc.
Embodiment
By the following examples the present invention is done further more detailed description.
Embodiment 1
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.0 * 10
9The enzymatic microorganism of CFU/g, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 300 grams, straw powder 100 grams, dried poultrymanure 100 grams, fish meal 50 grams, bone meal 10 grams, dregs of beans 50 grams, borax 300 grams, sal epsom 300 grams, ferrous sulfate 550 grams, potassium primary phosphate 50 grams, manganous sulfate 300 grams.
Bacterium is the mixed bacterium that is selected from Bacillus licheniformis, bacillus cereus and the pseudomonas; Yeast is Pichia yeast; Fungi is viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The fertile preparation technology of a kind of booth vegetable be used to preventing and treating disease may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1: 2: 1: 1 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 22 ℃ of incubators to cultivate chose bacterium in 3 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 2 times, was raised to 42 ℃ of beginning turnings through 24 hours temperature, once a day; 55 ℃ of heap temperature, turning 5 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, after b, raw material were piled up well, every day, thermometric twice, and be raised to 45 ℃ beginning turnings through 72 hours temperature winter, once a day; 60 ℃ of heap temperature, at once turning, turning 7 times.
Embodiment 2
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.0-3.0 * 10
9The enzymatic microorganism of CFU/g, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 600 grams, straw powder 300 grams, dried poultrymanure 350 grams, fish meal 100 grams, bone meal 50 grams, dregs of beans 100 grams, borax 800 grams, sal epsom 900 grams, ferrous sulfate 1000 grams, potassium primary phosphate 150 grams, manganous sulfate 1000 grams.
Bacterium is the mixed bacterium that is selected from bacillus cereus and pseudomonas; Yeast is Pichia yeast and intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The fertile preparation technology of a kind of booth vegetable be used to preventing and treating disease may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1: 1.5: 0.6: 0.5 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 22 ℃ of incubators to cultivate chose bacterium in 3 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 2 times, was raised to 42 ℃ of beginning turnings through 24 hours temperature, once a day; 55 ℃ of heap temperature, turning 5 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, after b, raw material were piled up well, every day, thermometric twice, and be raised to 45 ℃ beginning turnings through 72 hours temperature winter, once a day; 60 ℃ of heap temperature, at once turning, turning 7 times.
Embodiment 3
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.0-3.0 * 10
9The enzymatic microorganism of CFU/g, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 352 grams, straw powder 130 grams, dried poultrymanure 220 grams, fish meal 70 grams, bone meal 30 grams, dregs of beans 70 grams, borax 500 grams, sal epsom 400 grams, ferrous sulfate 600 grams, potassium primary phosphate 80 grams, manganous sulfate 500 grams.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and the pseudomonas; Yeast is Pichia yeast and intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The fertile preparation technology of a kind of booth vegetable be used to preventing and treating disease may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 2: 3: 2: 2 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 28 ℃ of incubators to cultivate chose bacterium in 7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 4 times, was raised to 45 ℃ of beginning turnings through 48 hours temperature, once a day; 60 ℃ of heap temperature, turning 8 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, after b, raw material were piled up well, every day, thermometric twice, and be raised to 42 ℃ beginning turnings through 72 hours temperature winter, once a day; 55 ℃ of heap temperature, at once turning, turning 5 times.
Embodiment 4
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.0 * 10
9The enzymatic microorganism of CFU/g, shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 425 grams, straw powder 178 grams, dried poultrymanure 300 grams, fish meal 90 grams, bone meal 40 grams, dregs of beans 80 grams, borax 750 grams, sal epsom 810 grams, ferrous sulfate 900 grams, potassium primary phosphate 100 grams, manganous sulfate 800 grams.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and pseudomonas; Yeast is Pichia yeast and/or intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The fertile preparation technology of a kind of booth vegetable be used to preventing and treating disease may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1: 1.5: 0.6: 0.5 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 22 ℃ of incubators to cultivate chose bacterium in 3 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 4 times, was raised to 42 ℃ of beginning turnings through 48 hours temperature, once a day; 60 ℃ of heap temperature, turning 8 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, after b, raw material were piled up well, every day, thermometric twice, and be raised to 45 ℃ beginning turnings through 72 hours temperature winter, once a day; 60 ℃ of heap temperature, at once turning, turning 6 times.
Embodiment 5
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.0 * 10
9The enzymatic microorganism of CFU/g, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 400 grams, straw powder 200 grams, dried poultrymanure 200 grams, fish meal 80 grams, bone meal 30 grams, dregs of beans 80 grams, borax 500 grams, sal epsom 700 grams, ferrous sulfate 900 grams, potassium primary phosphate 100 grams, manganous sulfate 700 grams.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and pseudomonas; Yeast is Pichia yeast and intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The fertile preparation technology of a kind of booth vegetable be used to preventing and treating disease may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1: 3: 0.6: 0.5 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 25 ℃ of incubators to cultivate chose bacterium in 5 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 3 times, was raised to 44 ℃ of beginning turnings through 40 hours temperature, once a day; 58 ℃ of heap temperature, turning 7 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, after b, raw material were piled up well, every day, thermometric twice, and be raised to 44 ℃ beginning turnings through 72 hours temperature winter, once a day; 58 ℃ of heap temperature, at once turning, turning 5-7 time.
Embodiment 6
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.5 * 10
9The enzymatic microorganism of CFU/g, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 50 grams, straw powder 150 grams, dried poultrymanure 350 grams, fish meal 100 grams, bone meal 50 grams, dregs of beans 100 grams, borax 800 grams, sal epsom 900 grams, ferrous sulfate 1000 grams, potassium primary phosphate 150 grams, manganous sulfate 300 grams.
Bacterium is one or more the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and the pseudomonas; Described yeast is Pichia yeast and/or intends the ball yeast; Described fungi is trichoderma harziarum and/or viride; Described actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The fertile preparation technology of a kind of booth vegetable be used to preventing and treating disease may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 2: 3: 2: 2 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 25 ℃ of incubators to cultivate chose bacterium in 5 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 3 times, was raised to 43 ℃ of beginning turnings through 30 hours temperature, once a day; 60 ℃ of heap temperature, turning 8 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, after b, raw material were piled up well, every day, thermometric twice, and be raised to 43 ℃ beginning turnings through 72 hours temperature winter, once a day; 57 ℃ of heap temperature, at once turning, turning 6 times.
Embodiment 7
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 3.0 * 10
9The enzymatic microorganism of CFU/g, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 352 grams, straw powder 150 grams, dried poultrymanure 220 grams, fish meal 70 grams, bone meal 30 grams, dregs of beans 75 grams, borax 500 grams, sal epsom 400 grams, ferrous sulfate 600 grams, potassium primary phosphate 80 grams, manganous sulfate 800 grams.
Bacterium is for being selected from subtilis; Yeast is Pichia yeast and intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1: 1.5: 0.6: 0.5 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 28 ℃ of incubators to cultivate chose bacterium in 7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 4 times, was raised to 42 ℃ of beginning turnings through 48 hours temperature, once a day; 60 ℃ of heap temperature, turning 8 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, after b, raw material were piled up well, every day, thermometric twice, and be raised to 45 ℃ beginning turnings through 72 hours temperature winter, once a day; 60 ℃ of heap temperature, at once turning, turning 7 times.
Embodiment 8
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.0 * 10
9The enzymatic microorganism of CFU/g; Enzymatic microorganism is bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 300 grams, straw powder 100 grams, dried poultrymanure 100 grams, fish meal 50 grams, bone meal 10 grams, dregs of beans 50 grams, borax 300 grams, sal epsom 300 grams, ferrous sulfate 550 grams, potassium primary phosphate 50 grams, manganous sulfate 300 grams.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis; Yeast is Pichia yeast and intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1: 1.5: 0.6: 0.5 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 22 ℃ of incubators to cultivate chose bacterium in 3 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 2 times, was raised to 42 ℃ of beginning turnings through 24 hours temperature, once a day; 55 ℃ of heap temperature, turning 5 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Embodiment 9
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 3.0 * 10
9The enzymatic microorganism of CFU/g; Enzymatic microorganism is bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 600 grams, straw powder 300 grams, dried poultrymanure 350 grams, fish meal 100 grams, bone meal 50 grams, dregs of beans 100 grams, borax 800 grams, sal epsom 900 grams, ferrous sulfate 1000 grams, potassium primary phosphate 150 grams, manganous sulfate 1000 grams.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and pseudomonas; Yeast is Pichia yeast; Fungi is trichoderma harziarum; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 2: 3: 2: 2 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 28 ℃ of incubators to cultivate chose bacterium in 7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric twice, and be raised to 45 ℃ beginning turnings through 72 hours temperature winter, once a day; 60 ℃ of heap temperature, at once turning, turning 7 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Embodiment 10
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.2 * 10
9The enzymatic microorganism of CFU/g; Enzymatic microorganism is bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 352 grams, straw powder 130 grams, dried poultrymanure 220 grams, fish meal 70 grams, bone meal 30 grams, dregs of beans 70 grams, borax 500 grams, sal epsom 400 grams, ferrous sulfate 600 grams, potassium primary phosphate 80 grams, manganous sulfate 500 grams.
Bacterium is the mixed bacterium that is selected from bacillus cereus and pseudomonas; Yeast is Pichia yeast and intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1.5: 2: 1: 1 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 25 ℃ of incubators to cultivate chose bacterium in 5 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric twice, and be raised to 42 ℃ beginning turnings through 72 hours temperature winter, once a day; 55 ℃ of heap temperature, at once turning, turning 5 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Embodiment 11
The booth vegetable that is used for preventing and treating disease of the present embodiment comprises that with fertile living bacteria count is 2.1 * 10
9The enzymatic microorganism of CFU/g, enzymatic microorganism are bacterium, yeast, fungi and actinomycetes.
The weight part of shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 400 grams, straw powder 150 grams, dried poultrymanure 250 grams, fish meal 80 grams, bone meal 35 grams, dregs of beans 75 grams, borax 600 grams, sal epsom 700 grams, ferrous sulfate 800 grams, potassium primary phosphate 90 grams, manganous sulfate 600 grams.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and pseudomonas; Yeast is Pichia yeast; Fungi is viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
Be used for preventing and treating the fertile preparation technology of booth vegetable of disease, may further comprise the steps:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, by 1: 1.5: 0.6: 0.5 volume ratio is made composite flora;
2) spawn culture
A, culture dish is placed in 28 ℃ of incubators to cultivate chose bacterium in 7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric twice, and be raised to 45 ℃ beginning turnings through 72 hours temperature winter, once a day; 60 ℃ of heap temperature, at once turning, turning 7 times;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
Should be noted that at last: above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit; although with reference to above-described embodiment the present invention is had been described in detail; those of ordinary skill in the field are to be understood that: the technician reads after the present specification still and can make amendment or be equal to replacement the specific embodiment of the present invention, does not await the reply within the claim protection domain but these modifications or change all break away from the present patent application.
Claims (8)
1. a booth vegetable that is used for preventing and treating disease is characterized in that comprising that with fertile living bacteria count is 2.0-3.0 * 10
9The enzymatic microorganism of CFU/g, shale, straw powder, dried poultrymanure, fish meal, bone meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate.
Enzymatic microorganism plays fermentative action, can so that " shale, straw powder, dried poultrymanure, fish meal, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate " slowly-releasing is put, can continue to allow these components play a role.
2. the booth vegetable be used to preventing and treating disease according to claim 1 is characterized in that with fertile described enzymatic microorganism is bacterium, yeast, fungi and actinomycetes.
3. the booth vegetable that is used for preventing and treating disease stated of the v of institute is characterized in that with fertile the weight part of described shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively according to claim 1:
Shale 300-600 part, straw powder 100-300 part, dried poultrymanure 100-350 part, fish meal 50-100 part, bone meal 10-50 part, dregs of beans 50-100 part, borax 300-800 part, sal epsom 300-900 part, ferrous sulfate 550-1000 part, potassium primary phosphate 50-150 part, manganous sulfate 300-1000 part.
4. the booth vegetable be used to preventing and treating disease according to claim 1 is characterized in that with fertile the weight part of described shale, straw powder, dried poultrymanure, bone meal, fish meal, dregs of beans, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate is respectively:
Shale 352-425 part, straw powder 130-178 part, dried poultrymanure 220-300 part, fish meal 70-90 part, bone meal 30-40 part, dregs of beans 70-80 part, borax 500-750 part, sal epsom 400-810 part, ferrous sulfate 600-900 part, potassium primary phosphate 80-100 part, manganous sulfate 500-800 part.
5. the booth vegetable be used to preventing and treating disease according to claim 2 is with fertile, it is characterized in that described bacterium is one or more the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus and the pseudomonas; Described yeast is Pichia yeast and/or intends the ball yeast; Described fungi is trichoderma harziarum and/or viride; Described actinomycetes are Streptomycin sulphate.
6. a Bacillus licheniformis claimed in claim 3 is characterized in that deposit number is CGMCC No 4920; The preservation name is called POLO-BLN1103.
7. the booth vegetable be used to preventing and treating disease according to claim 1 may further comprise the steps with fertile preparation technology:
1) preparation composite bacteria
With above-mentioned bacterium, yeast, fungi and actinomycetes, press (1-2): (1.5-3): (0.6-2): volume ratio (0.5-2) is made composite flora;
2) spawn culture
A, culture dish is placed in the 22-28 ℃ of incubator to cultivate chose bacterium in 3-7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously; Select regular single bacterium colony, be transferred to respectively on the slant medium, be cultured to and bacterium colony occurs, whether pure by the bacterium colony of observing on the 1. inclined-plane; 2. microscopy morphological features; And 3. pour the plane ware into by plate streaking or dilution and separate until purifying is selected purer bacterium colony;
B, preparation substratum
Potassium primary phosphate (KH
2PO
4) 1.0g, glucose (C
6H
12O
6H
2O) 10.0g, sal epsom (MgSO
47H
2O) 0.5g, peptone 5.0g, mass percent are 1% rose-bengal aqueous solution 3.3ml, paraxin 0.1g, agar 18.0g and distilled water 1000ml;
C, preparation
With the component raw material of above-mentioned weight part meter with dissolved in distilled water after, in wherein adding the step B mass percent 1% rose-bengal aqueous solution, then step paraxin is merged with dissolve with ethanol and mentioned solution and be added in the substratum of step B, add again agar, rear packing is dissolved in heating, 115 ℃ of sterilization 20min;
3) preparation fertilizer
A, above-mentioned shale, straw powder, dried poultrymanure, bone meal, dregs of beans by weight mixed, then composite bacteria is sprinkled upon above the said mixture equably, pile arch after stirring, cover fermentation;
After b, raw material were piled up well, every day, thermometric was 2-4 time, was raised to 42-45 ℃ of beginning turning through 24-48 hour temperature, once a day; Pile warm 55-60 ℃, turning 5-8 time;
C, fermentation are pulverized after finishing, and add above-mentioned borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight in the material that obtains after pulverizing.
8. the booth vegetable be used to preventing and treating disease according to claim 7 is characterized in that with fertile preparation technology:
After b, raw material were piled up well, every day, thermometric twice, and be raised to 42-45 ℃ beginning turning through 72 hours temperature winter, once a day; Pile warm 55-60 ℃, at once turning, turning 5-7 time.
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| CN201110250530.8A CN102951958B (en) | 2011-08-29 | 2011-08-29 | Anti-disease greenhouse vegetable fertilizer and preparation technology thereof |
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|---|---|---|---|
| CN201110250530.8A CN102951958B (en) | 2011-08-29 | 2011-08-29 | Anti-disease greenhouse vegetable fertilizer and preparation technology thereof |
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| CN102951958B CN102951958B (en) | 2014-12-03 |
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| CN105237250A (en) * | 2015-08-28 | 2016-01-13 | 安徽丰原马鞍山生物化学有限公司 | Green chemical fertilizer |
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| CN106431661A (en) * | 2016-09-28 | 2017-02-22 | 西南大学 | Preparation method and application of Pythium oligandrum microbial fertilizer |
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| CN107954765A (en) * | 2018-01-15 | 2018-04-24 | 福建三康循环农法农业科技有限公司 | A kind of fruits and vegetables special bio organic liquid fertilizer and preparation method |
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