CN111269844B - Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof - Google Patents
Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof Download PDFInfo
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Abstract
The invention discloses a Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof, wherein Trichoderma harzianum TW21990 is preserved in China general microbiological culture Collection center, and the preservation number is as follows: CGMCC No.12864, and the preservation date is 2016, 9 and 1. The method comprises the steps of taking a leek gray mold pathogen botrytis cinerea as a target, screening high-efficiency antagonistic trichoderma in a flat plate antagonistic mode, and then re-screening by a field plot experiment to obtain a trichoderma harzianum strain TW21990 for high-efficiency control of leek gray mold, wherein the control effect of the plot on leek gray mold is more than 90%, and a preparation for flushing application and spraying application is developed, and the control effect of the field application on leek gray mold is more than 85%.
Description
Technical Field
The invention relates to the technical field of biocontrol strains and application thereof, in particular to trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof.
Background
The gray mold of the leek is caused by botrytis cinerea of adelomycete, and the low-temperature and high-humidity environmental conditions of a protected area in winter are serious, the yield is reduced by 10-30% in a light case, and the greenhouse is destroyed and the crop is dead in a heavy case. According to the Chinese pesticide information network, only 15% of pythium fume-promoting agent and 50% of pythium wettable powder are used for preventing and controlling the gray mold of the Chinese chives, the safety interval period of the product is specified to be 30 days, but the growth period of a single crop of the Chinese chives in a protected area is generally different from 25-40 days, and if the single crop of the Chinese chives reaches the harvest standard, the product is harvested in time, the commodity is affected, and the economic benefit is reduced, so that the safety interval period of the pesticide is difficult to guarantee, which means that once the pythium is applied in the production of the facility Chinese chives, the risk of exceeding the standard of the pesticide.
In the prior art, the trichoderma has been well applied to preventing and treating the gray mold of cucumber and tomato, but the effect is poor in preventing and treating the gray mold of Chinese chives, so that the method for breeding trichoderma strains for efficiently preventing and treating the gray mold of the Chinese chives, researching an adaptive microbial inoculum and an application technology is a fundamental way for solving the problem of the drug residue of the Chinese chives.
Disclosure of Invention
The invention provides a Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof aiming at the defects.
The Trichoderma harzianum TW21990 for preventing and treating leek gray mold and the application technical scheme thereof are that the Trichoderma harzianum TW21990 for preventing and treating leek gray mold is classified and named as Trichoderma haroderma harzianum, and the biological materials are referred to as follows: TW21990, deposited in the general microbiological culture collection center of the china committee for the preservation of microorganisms, address: west road No.1, north chen, chaoyang district, beijing, zip code: 100101, the accession number is: CGMCC No.12864, and the preservation date is 2016, 9 and 1. It was identified as Trichoderma harzianum (Trichoderma harzianum) by morphological and ITS sequence analysis.
Trichoderma harzianum TW21990 has broad spectrum resistance, and has good antagonistic effect on Botrytis cinerea, Cytospora destructor, Fusarium solani, Rhizoctonia solani, Fusarium graminearum, Pythium ultimum and other 7 pathogenic fungi tested on PDA plate.
The plate antagonism 5-day inhibition rate of trichoderma reesei TW21990 on the gray mold of leeks reaches 97%, which is higher than the plate antagonism 5-day inhibition rate of a control strain LTR-2 on the gray mold of leeks.
The trichoderma harzianum TW21990 for preventing and treating leek gray mold can be applied to the preparation of a preparation for preventing and treating leek gray mold.
Trichoderma harzianum TW21990 produces conidium mother drug by fermentation, making pour-on Trichoderma powder and spray-on Trichoderma powder.
A spray-type wettable powder is characterized in that Trichoderma harzianum TW21990 prepares a conidium mother drug through fermentation, trehalose, xanthan gum and kieselguhr are added to prepare the spray-type wettable powder, and the effective spore content is 2 hundred million/g.
Preparing a conidium mother medicine from Trichoderma harzianum TW21990 by fermentation, and adding 2-5% of trehalose, 0.1-0.5% of xanthan gum and 93-96% of kieselguhr by weight percent of the mother medicine to prepare spray type Trichoderma powder.
A trichoderma russelinense powder is prepared by fermenting Trichoderma harzianum TW21990 to obtain conidium mother medicine, adding turfy soil or Maifanitum, and making into trichoderma russelinense powder with effective spore content of 2 hundred million/g
The trichoderma harzianum TW21990 prepares conidium mother medicine by fermentation, and 90-95 percent of turfy soil or 90-95 percent of medical stone according to the weight percentage of the mother medicine is added to prepare the washout type trichoderma powder.
The invention has the beneficial effects that:
the Trichoderma harzianum TW21990 has broad-spectrum resistance, and has better antagonistic action on 7 pathogenic fungi such as botrytis cinerea, cylindrosporium destructor, fusarium solani, rhizoctonia solani, fusarium graminearum, pythium ultimum and the like on a PDA (personal digital assistant) plate. The plate antagonism 5-day inhibition rate of the gray mold of the Chinese chives reaches 97 percent, which is higher than the plate antagonism 5-day inhibition rate of a control strain LTR-2 to the gray mold of the Chinese chives.
The microbial inoculum prepared by TW21990 for preventing and treating the gray mold of the Chinese chives has obvious prevention and treatment effect, 91.94 percent of prevention and treatment effect of the gray mold of the Chinese chives is continuously sprayed for 2 times, 86.81 percent of prevention and treatment effect of the microbial inoculum of trichoderma atroviride 23617 is equal to the effect; the control effect of the control strain LTR-2 is 64.14%, and the difference is obvious; there was also a significant difference (p <0.05) from other microbial inoculum treatments.
In a demonstration area (Yishui county Tianmu agriculture professional cooperative, Shandong province), before the leeks are buckled, Trichoderma harzianum TW21990 flushing type microbial inoculum is flushed with water, the using amount is 75 kg/hectare, when the leeks grow to about 5cm, a spray type Trichoderma harzianum TW21990 microbial inoculum is sprayed by an intelligent electric powder spraying machine, and the effect of controlling the gray mold of the leeks is investigated to reach 86.85% after the leeks are buckled for 1 month.
Description of the drawings:
FIG. 1 shows a photograph of a characteristic of a colony of Trichoderma strain TW21990 according to the invention;
FIG. 2 shows a photomicrograph of a conidiophore of Trichoderma strain TW21990 of the invention;
FIG. 3 shows a photomicrograph of conidia of Trichoderma strain TW21990 of the invention;
FIG. 4 is a plate bacteriostasis picture of Trichoderma reesei TW21990 on gray mold of Chinese chive.
The specific implementation mode is as follows:
for better understanding of the present invention, the technical solution of the present invention will be described in detail with specific examples, but the present invention is not limited thereto.
Example 1
A Trichoderma harzianum TW21990 for preventing and treating leek gray mold, is classified and named Trichoderma harzianum, and is referred to biological materials: TW21990, deposited in the general microbiological culture collection center of the china committee for the preservation of microorganisms, address: west road No.1, north chen, chaoyang district, beijing, zip code: 100101, the accession number is: CGMCC No.12864, and the preservation date is 2016, 9 and 1. It was identified as Trichoderma harzianum (Trichoderma harzianum) by morphological and ITS sequence analysis.
A colony characteristic photo of the trichoderma strain TW21990 is shown in the attached figure 1 of the specification, a conidiophor micrograph is shown in the attached figure 2 of the specification, and a conidiophor micrograph is shown in the attached figure 3 of the specification.
The Trichoderma strain TW21990ITS sequence, Tef1 sequence and Rpb2 sequence of the invention
TW21990(628bp)ITS
GTGATTAGATCTCGTTGGTGACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGGATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTATTGTATACCCCCTCGCGGGTTTTTTTTATAATCTGAGCCTTCTCGGCGCCTCTCGTAGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTGCCTTGGCGGTGGCCGTCTCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCGCGTCCACAGCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAAAAGGCGGGAGA
Tef1 TW21990(1291bp)
GAACTGAATTTTCGCTCGATTCTTCCTCCTCCACATTCAATTGTGCCCGACAATTCTGCAGAGAATTTTCGTGTCGACAATTTTTCATCACCCCGCTTTCCATTACCCCTCCTTTGCAGCGACGCAAATTTTTTTTGCTGTCGTTTGGTTTTTAGTGGGGTTCTCTGTGCAACCCCACTAGCTCCCTGCTTTTTCCTGCTTCACCTTCACTTCCTCGTCATCATTCAACGTGCTCTGCGTCTTTGGTCATTCAGCGACGCTAACCACTTTTCCATCAATAGGAAGCCGCCGAACTCGGCAAGGGTTCCTTCAAGTACGCTTGGGTTCTTGACAAGCTCAAGGCCGAGCGTGAGCGTGGTATCACCATTGACATTGCTCTGTGGAAGTTCGAGACTCCCAAGTACTATGTCACCGTCATTGGTAAGTCTTCACTAAGTTCATGCTGCAATTGCGGACCAGTGCTAACAGGCAATTCACAGACGCTCCCGGCCACCGTGATTTCATCAAGAACATGATCACTGGTACTTCCCAGGCCGATTGCGCTATCCTCATCATTGCCGCCGGTACTGGTGAGTTCGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCACGCTCTGCTCGCCTACACCCTGGGTGTTAAGCAGCTCATCGTTGCCATCAACAAGATGGACACTGCCAACTGGGCCGAGGCTCGTTACCAGGAAATCATCAAGGAGACTTCCAACTTCATCAAGAAGGTCGGCTTCAACCCCAAGGCTGTTGCTTTCGTCCCCATCTCCGGTTTCAACGGTGACAACATGCTCCAGCCCTCCACCAACTGCCCCTGGTACAAGGGCTGGGAGAAGGAGACCAAGGCTGGCAAGTTCACCGGCAAGACCCTCCTTGAGGCCATCGACTCCATCGAGCCCCCCAAGCGTCCCACGGACAAGCCCCTCCGTCTTCCCCTCCAGGATGTCTACAAGATCGGTGGTATTGGAACAGTTCCCGTCGGCCGTATCGAGACTGGTGTCCTCAAGCCCGGTATGGTTGTCACTTTCGCTCCCTCCAACGTCACCACTGAAGTCAAGTCCGTCGAGATGCACCACGAGCAGCTCGTCGAGGGTGTTCCCGGTGACAACGTTGGTTTCAACGTCAAGAACGTTTCCGTTAAGGAAATTCGCCGTGGTAACGTTGCCGGTGACTCCAAGAACGACCCCCCCATGGGTGCCGCTTCTTTCACCGCTCAGGTCATCGTCATGAACCACCCTGGCCAGGTCGGTGCCGGCTACGCCCCCGTCTGGTCCCAT
Rpb2(1184bp)：TW21990
AGGGCATTTGAAAACTGGGCGGGTCCTGCTGGCCAGCTGTTCCGTGGTATCATGCGAAGGATGAACACTGAGTTGGCCAACTACCTGAGACGATGCGTTGAGGGCAACCGACACTTCAACCTGGCTGTTGGTATCAAGCCCGGCACGCTTTCAAACGGACTGAAGTATTCGCTTGCCACAGGAAACTGGGGTGATCAGAAGAAGGCCATGAGCTCAACTGCCGGTGTGTCCCAGGTGCTTAACCGTTACACGTTTGCTTCGACCTTGTCACATTTGCGTCGTACCAACACTCCTATCGGGAGAGATGGTAAGCTGGCGAAGCCTCGACAGCTTCACAACACGCATTGGGGCTTGGTCTGCCCAGCCGAGACACCCGAAGGACAGGCCTGTGGTCTGGTCAAGAACTTGTCTTTGATGTGTTACGTCAGTGTCGGTTCTCCCTCCGAGCCTCTGATTGAGTTCATGATCAACAGAGGTATGGAAGTCGTCGAAGAGTACGAGCCGCTGCGGTATCCTCATGCTACAAAGATTTTTGTGAACGGTGTCTGGGTTGGAGTTCACCAAGACCCTAAGCACTTGGTGAACCAGGTTCTGGATACTCGTCGCAAGTCCTATCTGCAATACGAAGTCTCTCTCGTGAGAGAAATTCGAGACCAGGAATTCAAAATCTTTTCCGATGCAGGTCGTGTCATGCGACCAGTCTTTACCGTTCAGCAGGAAGATGATCCGGAAACGGGCATCAACAAGGGCCACCTGGTATTGACCAAGGAGCTCGTCAATAGATTGGCCAAGGAGCAGGCTGAGCCTCCGGAAGACCCCAGCATGAAGATTGGATGGGAGGGATTGATCAGGGCTGGTGCGGTTGAATATCTCGACGCCGAGGAAGAGGAGACGGCCATGATCTGCATGACACCAGAGGATCTCGAGCTGTATCGTCTTCAGAAGGCCGGTATCAACACTGAGGAAGACATGGGAGATGATCCGAACAAGCGACTCAAGACCAAGACGAACCCGACAACTCACATGTACACCCATTGCGAGATTCACCCAAGTATGATCTTAGGTATCTGTGCTAGTATCATTCCTTTCCCCGATCACAACCAGGTATGTTGTCCACGCTTTCAGTCTTATGAACGAAGAAAAAAACTAATGGTGTGCATATCCCCCCTAACATTATAATTTCA
Example 2
Screening of trichoderma strains for preventing and treating gray mold of Chinese chives on flat plate
Inoculating Trichoderma harzianum TW21990 and pathogenic fungi with diameter of 5mm at the positions 35mm away from the center at both ends of the PDA plate diameter, culturing at 25 deg.C for 3-5d with only inoculated pathogenic fungi as control, recording the growth radius of the pathogenic fungi in the opposite direction, and calculating the inhibition rate (%).
The inhibition ratio (%). 100% × (control pathogen growth radius-treatment pathogen growth radius)/control pathogen growth radius.
The results are shown in FIG. 4 of the drawings attached to the specification in Table 1:
TABLE 1 plate inhibition of Botrytis cinerea by Trichoderma harzianum TW21990 strain (20 ℃ C.)
As can be seen from the table, the inhibition rate of Trichoderma harzianum TW 219906 to botrytis cinerea plate is 97.00%, compared with the control strain LTR-2 (preservation number: CGMCC No.1498, Trichoderma viride LTR-2), the effect is equivalent, and no significant difference exists between the control strain and other strains.
Example 3
Trichoderma harzianum TW21990 produces conidium mother drug by fermentation, making pour-on Trichoderma powder and spray-on Trichoderma powder.
A spray type wettable powder is characterized in that Trichoderma harzianum TW21990 prepares a conidium mother medicine through fermentation, 2% -5% trehalose, 0.1% -0.5% xanthan gum and 93% -96% diatomite by weight of the mother medicine are added to prepare the spray type Trichoderma powder, and the effective spore content is 2 hundred million/g.
A trichoderma russelinense powder for flush application is prepared through fermenting Trichoderma harzianum TW21990 to obtain conidium mother medicine, adding peat soil 90-95 wt% or Chinese medicinal stone 90-95 wt% to obtain trichoderma reesei powder with effective spore content of 2 hundred million/g
Example 4
Field plot rescreening high-efficiency trichoderma for preventing and treating leek gray mold
The test is carried out in a greenhouse of Tianmu agriculture professional cooperative society in Yishui county of Shandong province, wherein the greenhouse is a winter-heating simple greenhouse. The variety of the planted leek is 'the King of leek in Hanzhong'.
3 kinds of trichoderma: trichoderma atroviride HB20111 with the preservation number: CGMCC No. 16963; trichoderma viride LTR-2 with the preservation number: CGMCC No. 1498; the Trichoderma harzianum TW21990 (live spore ≧ 2 hundred million/g) of the present invention.
The inoculum preparation is as in example 3.
The experiment was set up for 3 treatments, each treatment was repeated 3 times, each cell area was 84m2(12m x 7m), the experiment was performed using a random block design, and a blank control was set up.
The 3 trichoderma preparations are all mixed uniformly by 300 times of liquid, the dosage is 750 g/hectare, the water consumption is 450L/hectare, and the mixture is uniformly mixed by using an intelligent electric sprayer to spray stem leaf surfaces and ground surfaces as much as possible. Spraying the leeks with the pesticide for 1 time every 7 days, wherein the pesticide is sprayed for two times. The first spraying time is 1 month and 3 days in 2019. The second spraying time is 1 month and 10 days in 2019.
The disease condition base survey was conducted before the first spray, and was conducted every 7 days. The first survey time was 2019, 1 month, 3 days (disease base survey); the 2 nd survey time is 2019, 1 month and 10 days; the 3 rd survey time was 2019, 1 month, and 17 days.
Sampling is carried out by adopting a 5-point sampling method, 3-5 leeks are randomly investigated at each point, and all leaves of each leek are investigated. Grading standard of gray mold diseased leaves of Chinese chives: grade 0, no scab; grade 1, but 1-3 scabs exist on the leaves; grade 3, 4-6 scabs exist on a single leaf; grade 5, 7-10 scabs exist on a single leaf; 7-grade, wherein 11-20 scabs exist in a single leaf, and the part is densely divided into pieces; and 9 grades, the area of the single blade with the scab is more than 1/4. The disease index and the prevention effect are calculated according to the pesticide field efficacy test criterion (I) that the bactericide prevents and treats the gray mold of vegetables.
Disease index [ sigma (number of diseased plants at each stage × relative grade value)/(total number of investigated plants × 9) ] × 100
Control effect (%) [ (blank control area disease finger-treatment area disease finger)/blank control area disease finger ] × 100
The results were first subjected to data statistics using Microsoft Office Excel 2010 and then to LSD analysis of variance using software SPSS 17.0.
The results are shown in Table 2.
TABLE 2 field control of leek Botrytis cinerea by Trichoderma
Note: the different lower case letters in the same column indicate significant differences between treatments (P <0.05).
As seen from the table, the control effect of trichoderma harzianum TW21990 on the gray mold of leeks reaches 91.94 percent, and is 86.81 percent, which is equivalent to the control effect of trichoderma atroviride TW 23617; the control effect of the Trichoderma harzianum LTR-2 strain is 64.14 percent compared with that of a control strain, the difference is obvious, and the control effect of the Trichoderma harzianum LTR-2 strain is also obvious different from that of other strains.
Sequence listing
<110> institute of ecology of academy of sciences of Shandong province
<120> Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 628
<212> DNA
<213> Trichoderma harzianum (Trichoderma harzianum)
<400> 1
gtgattagat ctcgttggtg accagcggag ggatcattac cgagtttaca actcccaaac 60
ccaatgtgaa cgttaccaaa ctgttgcctc ggcgggatct ctgccccggg tgcgtcgcag 120
ccccggacca aggcgcccgc cggaggacca accaaaactc ttattgtata ccccctcgcg 180
ggtttttttt ataatctgag ccttctcggc gcctctcgta ggcgtttcga aaatgaatca 240
aaactttcaa caacggatct cttggttctg gcatcgatga agaacgcagc gaaatgcgat 300
aagtaatgtg aattgcagaa ttcagtgaat catcgaatct ttgaacgcac attgcgcccg 360
ccagtattct ggcgggcatg cctgtccgag cgtcatttca accctcgaac ccctccgggg 420
ggtcggcgtt ggggatcggc cctgccttgg cggtggccgt ctccgaaata cagtggcggt 480
ctcgccgcag cctctcctgc gcagtagttt gcacactcgc atcgggagcg cggcgcgtcc 540
acagccgtta aacacccaac ttctgaaatg ttgacctcgg atcaggtagg aatacccgct 600
gaacttaagc atatcaaaag gcgggaga 628
<210> 3
<211> 1291
<212> DNA
<213> Trichoderma harzianum (Trichoderma harzianum)
<400> 3
gaactgaatt ttcgctcgat tcttcctcct ccacattcaa ttgtgcccga caattctgca 60
gagaattttc gtgtcgacaa tttttcatca ccccgctttc cattacccct cctttgcagc 120
gacgcaaatt ttttttgctg tcgtttggtt tttagtgggg ttctctgtgc aaccccacta 180
gctccctgct ttttcctgct tcaccttcac ttcctcgtca tcattcaacg tgctctgcgt 240
ctttggtcat tcagcgacgc taaccacttt tccatcaata ggaagccgcc gaactcggca 300
agggttcctt caagtacgct tgggttcttg acaagctcaa ggccgagcgt gagcgtggta 360
tcaccattga cattgctctg tggaagttcg agactcccaa gtactatgtc accgtcattg 420
gtaagtcttc actaagttca tgctgcaatt gcggaccagt gctaacaggc aattcacaga 480
cgctcccggc caccgtgatt tcatcaagaa catgatcact ggtacttccc aggccgattg 540
cgctatcctc atcattgccg ccggtactgg tgagttcgag gctggtatct ccaaggatgg 600
ccagacccgt gagcacgctc tgctcgccta caccctgggt gttaagcagc tcatcgttgc 660
catcaacaag atggacactg ccaactgggc cgaggctcgt taccaggaaa tcatcaagga 720
gacttccaac ttcatcaaga aggtcggctt caaccccaag gctgttgctt tcgtccccat 780
ctccggtttc aacggtgaca acatgctcca gccctccacc aactgcccct ggtacaaggg 840
ctgggagaag gagaccaagg ctggcaagtt caccggcaag accctccttg aggccatcga 900
ctccatcgag ccccccaagc gtcccacgga caagcccctc cgtcttcccc tccaggatgt 960
ctacaagatc ggtggtattg gaacagttcc cgtcggccgt atcgagactg gtgtcctcaa 1020
gcccggtatg gttgtcactt tcgctccctc caacgtcacc actgaagtca agtccgtcga 1080
gatgcaccac gagcagctcg tcgagggtgt tcccggtgac aacgttggtt tcaacgtcaa 1140
gaacgtttcc gttaaggaaa ttcgccgtgg taacgttgcc ggtgactcca agaacgaccc 1200
ccccatgggt gccgcttctt tcaccgctca ggtcatcgtc atgaaccacc ctggccaggt 1260
cggtgccggc tacgcccccg tctggtccca t 1291
<210> 2
<211> 1184
<212> DNA
<213> Trichoderma harzianum (Trichoderma harzianum)
<400> 2
agggcatttg aaaactgggc gggtcctgct ggccagctgt tccgtggtat catgcgaagg 60
atgaacactg agttggccaa ctacctgaga cgatgcgttg agggcaaccg acacttcaac 120
ctggctgttg gtatcaagcc cggcacgctt tcaaacggac tgaagtattc gcttgccaca 180
ggaaactggg gtgatcagaa gaaggccatg agctcaactg ccggtgtgtc ccaggtgctt 240
aaccgttaca cgtttgcttc gaccttgtca catttgcgtc gtaccaacac tcctatcggg 300
agagatggta agctggcgaa gcctcgacag cttcacaaca cgcattgggg cttggtctgc 360
ccagccgaga cacccgaagg acaggcctgt ggtctggtca agaacttgtc tttgatgtgt 420
tacgtcagtg tcggttctcc ctccgagcct ctgattgagt tcatgatcaa cagaggtatg 480
gaagtcgtcg aagagtacga gccgctgcgg tatcctcatg ctacaaagat ttttgtgaac 540
ggtgtctggg ttggagttca ccaagaccct aagcacttgg tgaaccaggt tctggatact 600
cgtcgcaagt cctatctgca atacgaagtc tctctcgtga gagaaattcg agaccaggaa 660
ttcaaaatct tttccgatgc aggtcgtgtc atgcgaccag tctttaccgt tcagcaggaa 720
gatgatccgg aaacgggcat caacaagggc cacctggtat tgaccaagga gctcgtcaat 780
agattggcca aggagcaggc tgagcctccg gaagacccca gcatgaagat tggatgggag 840
ggattgatca gggctggtgc ggttgaatat ctcgacgccg aggaagagga gacggccatg 900
atctgcatga caccagagga tctcgagctg tatcgtcttc agaaggccgg tatcaacact 960
gaggaagaca tgggagatga tccgaacaag cgactcaaga ccaagacgaa cccgacaact 1020
cacatgtaca cccattgcga gattcaccca agtatgatct taggtatctg tgctagtatc 1080
attcctttcc ccgatcacaa ccaggtatgt tgtccacgct ttcagtctta tgaacgaaga 1140
aaaaaactaa tggtgtgcat atccccccta acattataat ttca 1184
Claims (5)
1. The application of Trichoderma harzianum TW21990 for preventing and treating leek gray mold in preparing a preparation for preventing and treating leek gray mold is characterized in that Trichoderma harzianum TW21990 is classified and named as Trichoderma harzianumTrichoderma harzianumThe biological materials referred to are: TW21990, deposited in the China Committee for culture Collection of microorganisms, the accession number of which is: CGMCC No.12864, with a preservation date of 2016, 9 months and 1 day; trichoderma harzianum TW21990 produces conidium mother drug by fermentation, making pour-on Trichoderma powder and spray-on Trichoderma powder.
2. The use of claim 1, wherein Trichoderma harzianum TW21990 is prepared into spray Trichoderma powder by fermenting conidia mother drug, adding trehalose, xanthan gum and diatomite, and has effective spore content of 2 hundred million/g.
3. The use of claim 2, wherein the trichoderma harzianum TW21990 is prepared into conidium mother drug by fermentation, and the spray type trichoderma powder is prepared by adding 2-5% of trehalose, 0.1-0.5% of xanthan gum and 93-96% of diatomite by weight percentage of the mother drug.
4. The use of claim 1, wherein Trichoderma harzianum TW21990 is fermented to produce a conidium mother drug, and Trichoderma reesei powder for fluxifragm is prepared by adding turfy soil or Maifanitum, and the effective spore content is 2 hundred million/g.
5. The use of claim 4, wherein the Trichoderma harzianum TW21990 is prepared into conidium mother medicine by fermentation, and Trichoderma russelinum powder is prepared by adding 90% -95% peat soil or 90% -95% Maifanitum by weight of the mother medicine.
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| CN113678844A (en) * | 2021-08-17 | 2021-11-23 | 山东五福生生态工程有限公司 | Trichoderma harzianum dispersible oil suspension and application thereof |
| CN114907986B (en) * | 2022-04-28 | 2022-11-18 | 云南省农业科学院生物技术与种质资源研究所 | Trichoderma harzianum and application thereof in preparation for preventing and treating root rot of panax notoginseng |
| CN114790427B (en) * | 2022-06-21 | 2022-08-26 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Trichoderma harzianum QT20724 and application thereof |
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