CN109221255A - The application of trichoderma LTR-2 and its microbial inoculum and preparation method - Google Patents
The application of trichoderma LTR-2 and its microbial inoculum and preparation method Download PDFInfo
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- CN109221255A CN109221255A CN201811043651.3A CN201811043651A CN109221255A CN 109221255 A CN109221255 A CN 109221255A CN 201811043651 A CN201811043651 A CN 201811043651A CN 109221255 A CN109221255 A CN 109221255A
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- wheat
- microbial inoculum
- amino acid
- trichoderma
- aleuronat
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
Trichoderma LTR-2 conidia powder and wheat seed are dressed seed, are then planted by application and its microbial inoculum and preparation method the invention discloses trichoderma LTR-2, application of the specially trichoderma LTR-2 in terms of improving aleuronat and amino acid content.The present invention is applied to wheat planting industry for Bio-control Trichoderma LTR-2, can prevent and treat wheat Some Disease, reduces the usage amount of fertilizer and pesticide, protects environment；Wheat per mu yield is improved, wheat output ratio is increased；Wheat Caryopsis Amino acid and protein content is improved, wheat quality is promoted.
Description
Technical field
The present invention relates to microbial bacterial agent applied technical field, more particularly to the application of trichoderma LTR-2 and its microbial inoculum and
Preparation method.
Background technique
Wheat is a kind of gramineae plant planted extensively all over the world, and the caryopsis of wheat is the staple food of the mankind, but leaf
Portion's disease and root disease have seriously threatened improving yield of wheat and wheat quality.Usual people are with chemical prevention and use resistant variety
Based on, the mode for being aided with cultivation management is administered.Though chemical agent effect is got instant result, it is also easy to produce drug-fast strain, and
And it largely is be easy to cause environmental pollution using these chemical pesticides for a long time, it is dangerous to non-target crops.Breeding and use are disease-resistant
Kind is a kind of cost-effective measure, but is widely applied the kind with single resistance on producing, so that varietal resistance
It loses in a relatively short period of time, to increase difficulty for breeding for disease resistance work.
With the raising that ecological, environmental protective is realized, agricultural sustainable development requires to take new disease control strategy, this will
It asks us to seek the economic and environment-friendly mode of one kind to prevent and treat.Wherein, the biological control research field new as one has become
One of the important means of crop disease is controlled, since biocontrol microorganisms have no pollution to the environment, residual quantity is low, and germ is not likely to produce anti-
Pharmacological property and it is safer to non-target plant the features such as, be considered an environment amenable green prevention and control technology.And at present
It is found that antagonistic microbe in, trichoderma bacterium has become a kind of important biocontrol of plant disease microbial inoculum.A variety of microbial inoculums
Commercialization scientific research institutions and enterprise certainly will be allowed to carry out more deep comprehensive understanding to trichoderma attribute.
Bio-control Trichoderma according to the present invention is Trichoderma viride Trichoderma viride LTR-2, and deposit number is
CGMCC NO.1498, the bacterial strain and its application in disease flocking biocontrol are the special of CN100340655C in Authorization Notice No.
It is disclosed in benefit, which has apparent inhibiting effect to various plants pathogens such as wheat root-rot sickle-like bacteria, the long compacted precious spore bacterium of wheat；Separately
The outer trichoderma strain has growth-promoting functions to cucumber, impregnates cucumber seeds with the microbial inoculum suspension, whole plant dry weight increases than control
61.5%.The bacterial strain is further identified from molecular biology angle at present, the present invention will change bacterial strain and be newly defined as Ha Ci
Trichoderma LTR-2, hereinafter referred to as trichoderma LTR-2.
Diseases prevention and growth-promoting are concentrated mainly on functionally to the research of trichoderma LTR-2 in currently available technology, are not directed to pair
The concern of nutritional quality.How using trichoderma LTR-2 microbial inoculum is prepared, except disease prevention growth-promoting function, further develops the bacterial strain
Characteristic is the technical problem to be solved in the present invention.
Summary of the invention
The application of present invention offer trichoderma LTR-2 and its microbial inoculum and preparation method.Microbial inoculum of the invention can prevent and treat wheat
Some Disease reduces the usage amount of fertilizer and pesticide, protects environment, improves wheat per mu yield, moreover it is possible to promote caryopsis amino acid content
Increase, improves crop nutritional quality.
The application of trichoderma LTR-2 of the invention and its microbial inoculum and preparation method technical solution are that trichoderma LTR-2 is small in raising
Application in terms of aleuronat matter and amino acid content.
Trichoderma LTR-2 conidia powder and wheat seed are dressed seed, then planted.
A kind of microbial inoculum improving aleuronat and amino acid content, trichoderma LTR-2 conidia powder is contained in the microbial inoculum.
The effective concentration of the microbial inoculum is that every wheat seed attachment trichoderma LTR-2 spore spore count is 104-107CFU。
A kind of microbial inoculum improving aleuronat and amino acid content, including following component: trichoderma LTR-2 conidia powder, carboxylic
Sodium carboxymethylcellulose pyce, sodium alginate, calcium chloride, medical stone.
The microbial inoculum of a kind of the raising aleuronat and amino acid content, is made of following component by weight:
5-10 parts of trichoderma LTR-2 conidia powder, 2-5 parts of sodium carboxymethylcellulose, 1-4 parts of sodium alginate, 1-3 parts of calcium chloride, medical stone 78-
91 parts.
Calcium chloride is ground up, sieved 80 mesh, medical stone 60-80 mesh.
A method of aleuronat and amino acid content are improved, is mixed using the microbial inoculum and wheat seed
Kind, then planted.
It is converted according to the wheat usage amount of the effective spore count and 0.07 hectare that contain in microbial inoculum, makes every wheat seed
Trichoderma LTR-2 spore number reach 104-107CFU/ every.
Preferably, in the present invention, the trichoderma LTR-2 spore number of every wheat seed is made to reach 105-106CFU/ every.
The use of trichoderma is not that concentration is the bigger the better, and Different Crop, Trichoderma dosage are also not quite similar.Room
In interior experiment, by wheat seed seed soaking Trichoderma spore liquid, it is allowed to every adherency 104-108Trichoderma spore, as a result, it has been found that 10
8 power concentration under wheat germination rate decline instead, therefore big Tanaka we be typically chosen 104-107CFU/ every, present invention choosing
Select 105-106CFU/ every.This concentration range is ideal in big Tanaka's effect.
The invention has the benefit that
1. wheat Some Disease can be prevented and treated, the usage amount of fertilizer and pesticide is reduced, protects environment；
2. improving wheat per mu yield, increase wheat output ratio；
3. improving Wheat Caryopsis Amino acid and protein content, wheat quality is promoted.
Specific embodiment:
For a better understanding of the present invention, below with specific example come the technical solution that the present invention will be described in detail, but this
Invention is not limited thereto.
Trichoderma viride Trichoderma viride LTR-2 according to the present invention, deposit number are CGMCC NO.1498,
The preparation method of the bacterial strain and its conidia powder discloses in the patent that Authorization Notice No. is CN100340655C.At present from point
Further identification of the sub- biology angle to the bacterial strain, the present invention will change bacterial strain and be newly defined as Trichoderma harzianum LTR-2, simple below
Claim trichoderma LTR-2.
Embodiment 1
A kind of microbial inoculum improving aleuronat and amino acid content, trichoderma LTR-2 conidia powder is contained in the microbial inoculum.
It is dressed seed using the microbial inoculum and wheat seed, is then planted.According to the effective spore count contained in microbial inoculum and one
The wheat usage amount on mu ground converts, and the trichoderma LTR-2 spore number of every wheat seed is made to reach 104-107CFU/ every.
Embodiment 2
A kind of microbial inoculum improving aleuronat and amino acid content, is made of following component by weight:
8 parts of trichoderma LTR-2 conidia powder
3 parts of sodium carboxymethylcellulose
3 parts of sodium alginate
2 parts of calcium chloride
84 parts of medical stone.
1. microbial inoculum field application method:
Microbial inoculum made from embodiment 1-3 and wheat seed are dressed seed, according to the effective spore contained in trichoderma
Several and 0.07 hectare wheat usage amount converts, and the trichoderma of every wheat seed spore count living is made to reach 105(the mark of CFU/ every
It is denoted as L-5 and means that the trichoderma of wheat seed spore living is 105), 106CFU/ every (means Wheat Species labeled as L-6
The trichoderma of son spore living is 106)。
3 different disposals are arranged in field experiment: space management: being not added with any microbial inoculum；Microbial inoculum of the present invention is added, setting is not
Same concentration gradient (makes every wheat seed adhere to 10 respectively5With 106The microbial inoculum of concentration).
2. hydrolysis amino acid measuring method (17 kinds of amino acid, in addition totally 18 kinds of tryptophan):
17 kinds of amino acid of wheat refer to aspartic acid, threonine, serine, glutamic acid, glycine, alanine, Guang ammonia
Acid, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine and dried meat ammonia
Acid；Along with tryptophan, amount to 18 kinds of hydrolysising amino acid contents of measurement.
(1) sample pretreating method: for the uniformity and hydrolysis effect for guaranteeing sample, the wheat seed after harvest is carried out
It smashes, crosses 80 mesh sieve.
(2) 17 kinds of determined amino acid methods
Hydrochloric Acid Hydrolysis Method, referring to GB7649-87 (1987)
Amino-acid analyzer: hundred health amino-acid analyzer Biochrom 30 of Britain
(3) Method For The Determination of Tryptophane
Alkali hydrolysis method, referring to GB7650-87 (1987)
Tryptophan analysis instrument: ultraviolet specrophotometer 722
3. method of protein measurement
The preposition processing of sample and hydrolysis amino acid measurement, sample grinds sieving, guarantees the uniformity and test effect.
Triumphant formula nitriding is provided referring to GB/T5511-2008
Instrument title: kjeldahl apparatus SKD-1000.
Table 1 is using wheat per mu yield schematic table after 2 microbial inoculum of the embodiment of the present invention；
Table 2 is using Protein Content in Wheat schematic table after 2 microbial inoculum of the embodiment of the present invention；
Table 3 is using wheat amino acid content schematic table after 2 microbial inoculum of the embodiment of the present invention.
After the application microbial inoculum processing of table 1, wheat per mu yield after harvest
The measurement of 2 Protein Content in Wheat of table
Table 3 wheat, 18 kinds of hydrolysis amino acid measurement results
Embodiment 3
A kind of microbial inoculum improving aleuronat and amino acid content, is made of following component by weight:
5 parts of trichoderma LTR-2 conidia powder
2 parts of sodium carboxymethylcellulose
1 part of sodium alginate
1 part of calcium chloride
91 parts of medical stone.
Microbial inoculum field application method and hydrolysis amino acid, method of protein measurement with embodiment 1,
Table 4 is using wheat per mu yield schematic table after 3 microbial inoculum of the embodiment of the present invention；
Table 5 is using Protein Content in Wheat schematic table after 3 microbial inoculum of the embodiment of the present invention；
Table 6 is using wheat amino acid content schematic table after 3 microbial inoculum of the embodiment of the present invention.
After the application microbial inoculum processing of table 4, wheat per mu yield after harvest
The measurement of 5 Protein Content in Wheat of table
Table 6 wheat, 18 kinds of hydrolysis amino acid measurement results
Embodiment 4
A kind of microbial inoculum improving aleuronat and amino acid content, is made of following component by weight:
10 parts of trichoderma LTR-2 conidia powder
5 parts of sodium carboxymethylcellulose
4 parts of sodium alginate
3 parts of calcium chloride
78 parts of medical stone.
Microbial inoculum field application method and hydrolysis amino acid measuring method with embodiment 1,
Table 7 is using wheat per mu yield schematic table after 4 microbial inoculum of the embodiment of the present invention；
Table 8 is using Protein Content in Wheat schematic table after 4 microbial inoculum of the embodiment of the present invention；
Table 9 is using wheat amino acid content schematic table after 4 microbial inoculum of the embodiment of the present invention.
After the application microbial inoculum processing of table 7, wheat per mu yield after harvest
The measurement of 8 Protein Content in Wheat of table
Table 9 wheat, 18 kinds of hydrolysis amino acid measurement results
Claims (10)
1. application of the trichoderma LTR-2 in terms of improving aleuronat and amino acid content.
2. application according to claim 1, which is characterized in that trichoderma LTR-2 conidia powder and wheat seed are dressed seed,
Then it is planted.
3. a kind of microbial inoculum for improving aleuronat and amino acid content, which is characterized in that contain trichoderma LTR-2 in the microbial inoculum
Conidia powder.
4. a kind of microbial inoculum for improving aleuronat and amino acid content according to claim 1, which is characterized in that the bacterium
The effective concentration of agent is that the spore count of every wheat seed attachment trichoderma LTR-2 spore is 104-107CFU。
5. a kind of microbial inoculum for improving aleuronat and amino acid content according to claim 1, which is characterized in that including
Following component: trichoderma LTR-2 conidia powder, sodium carboxymethylcellulose, sodium alginate, calcium chloride, medical stone.
6. a kind of microbial inoculum for improving aleuronat and amino acid content according to claim 5, which is characterized in that by pressing
The following component of parts by weight forms: 5-10 parts of trichoderma LTR-2 conidia powder, 2-5 parts of sodium carboxymethylcellulose, 1-4 parts of sodium alginate,
1-3 parts of calcium chloride, 78-91 parts of medical stone.
7. a kind of microbial inoculum for improving aleuronat and amino acid content according to claim 5, which is characterized in that chlorination
Calcium is ground up, sieved 80 mesh, medical stone 60-80 mesh.
8. a kind of method for improving aleuronat and amino acid content, which is characterized in that use microbial inoculum described in claim 1
It dresses seed with wheat seed, is then planted.
9. a kind of method for improving aleuronat and amino acid content according to claim 8, which is characterized in that according to
The wheat usage amount of the effective spore count and 0.07 hectare that contain in microbial inoculum converts, and makes the trichoderma LTR-2 of every wheat seed
Spore number reaches 104-107CFU/ every.
10. a kind of method for improving aleuronat and amino acid content according to claim 8, which is characterized in that every
The trichoderma LTR-2 spore number of grain wheat seed reaches 105-106CFU/ every.
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| CN109628323A (en) * | 2018-12-28 | 2019-04-16 | 云南星耀生物制品有限公司 | Trichoderma Viride method and its water dispersible granules |
| CN110810174A (en) * | 2019-11-15 | 2020-02-21 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Application of trichoderma harzianum LTR-2 in cereal crop cultivation |
| CN111269844A (en) * | 2020-04-02 | 2020-06-12 | 山东省科学院生态研究所 | Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof |
| CN113367160A (en) * | 2021-06-09 | 2021-09-10 | 云南星耀生物制品有限公司 | Application of trichoderma viride preparation in preventing and treating tomato gray mold |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109628323A (en) * | 2018-12-28 | 2019-04-16 | 云南星耀生物制品有限公司 | Trichoderma Viride method and its water dispersible granules |
| CN109628323B (en) * | 2018-12-28 | 2019-09-27 | 云南星耀生物制品有限公司 | Trichoderma Viride method and its water dispersible granules |
| CN110810174A (en) * | 2019-11-15 | 2020-02-21 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Application of trichoderma harzianum LTR-2 in cereal crop cultivation |
| CN110810174B (en) * | 2019-11-15 | 2021-12-24 | 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) | Application of trichoderma harzianum LTR-2 in cereal crop cultivation |
| CN111269844A (en) * | 2020-04-02 | 2020-06-12 | 山东省科学院生态研究所 | Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof |
| CN111269844B (en) * | 2020-04-02 | 2021-05-25 | 山东省科学院生态研究所 | Trichoderma harzianum TW21990 for preventing and treating leek gray mold and application thereof |
| CN113367160A (en) * | 2021-06-09 | 2021-09-10 | 云南星耀生物制品有限公司 | Application of trichoderma viride preparation in preventing and treating tomato gray mold |
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