CN102895526A - Externally-applied skin-generating oil for treatment of burn and preparation method and application thereof - Google Patents
Externally-applied skin-generating oil for treatment of burn and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of a traditional Chinese medicine preparation, and particularly discloses externally-applied skin-generating oil for treatment of burns and a preparation method and application thereof. The skin-generating oil is prepared by soaking the following 15 Chinese herbs in sesame oil: radix scutellariae, rheum officinale, astragalus mongholicus, coptis chinensis, cortex phellodendri, angelica sinensis, sanguisorba officinalis, lithospermum, rehmannia, radix angelicae, cynanchum atratum, bletilla striata, olibanum, myrrh and borneol. When the skin-generating oil is applied in treatment of burns, wound infection caused after injury can be prevented effectively, proliferation and migration of fibroblasts and vascular endothelial cells can be promoted, neovascularization can be induced, wound healing can be accelerated, scar repair can be promoted, the wound healing time can be shortened, Class I-II burns can be cured in 10-12 days, and Class III burns of rabbits can be cured in 20-25 days. When Class III burns of rabbits are treated with the skin-generating oil, the wound area can be reduced from 6 cm<2> to 0.1cm<2>, the wound surface is flat, smooth and soft, and newborn hair is normal. The wound epidermis can be recovered completely, dense hair follicles can be formed in the dermis layer, and almost no difference from normal skin tissues can be produced.
Description
Technical field
The present invention relates to the Chinese medicine preparation technical field, relate in particular to a kind of external for the treatment of burn and scald and give birth to skin oil and its preparation method and application.
Background technology
China's burn and scald Annual occurence rate accounts for 0.5%～1.0% of total population at present according to statistics, and the burn and scald disease is a kind of somatic damage that is mainly caused by Gao Re or calcination.According to the degree of burn and scald, burn and scald generally can be divided into I degree, shallow II degree, dark II degree and III degree burn and scald.Wherein clinically relatively easily control and treatment of I degree and II degree burn and scald, and III degree burn and scald is the damage of skin holostrome, that have even injure subcutaneous tissue, muscle and skeleton etc., wound surface easily infects after hindering, often form cicatrix in various degree after the healing, sometimes also scar hyperplasia and muscle contraction can occur.According to statistics, the disability rate of China's large-area burns reaches 48% at present, and wherein between twenty and fifty disability rate is about 78.1%(burn wound therapeutic strategy. China burn magazine .2011,27:247-249).The burn and scald grievous injury China people's physical and mental health and economic interests.Wherein the treatment reparation of III degree empyrosis wound surface skin becomes one of present problem demanding prompt solution especially.
At present adopt the dosage forms such as ointment of doctor trained in Western medicine to treat to the treatment of I～II degree burn and scald clinically more, but there is certain side effect in this therapeutic modality, and some wound surface exists treatment thoroughly consequently not recur and problem (Chinese patent application 201010516285.6) repeatedly; Meanwhile, adopt dermatoplastic method to the treatment of III degree burn and scald is normal clinically, but cost is higher, treatment time is longer.Effectively the Chinese medicine preparation for the treatment of III degree burn and scald is relatively deficient on the present stage market, most of medicine timely Qinghuo Jiedu after use, and alleviating pain and detumescence easily stays cicatrix behind the wound healing, still need to do cosmetic surgery in the future.The Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum are usually used in treating burn and scald, but mostly be used for the treatment of I degree and II degree burn and scald during single or several use of these medicines, be rarely used in III degree burn and scald treatment (China burn is complete works of. Beijing: the .1998:234-257 of publishing house of institute).15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum are not made preparation at present, for the preparation of the relevant report for the treatment of III degree burn and scald medicament yet.
Summary of the invention
For the deficiencies in the prior art, first purpose of the present invention has been to provide a kind of external for the treatment of burn and scald to give birth to skin oil.
Second purpose of the present invention has been to provide a kind of external of above-mentioned treatment burn and scald to give birth to the preparation method of skin oil.
The 3rd purpose of the present invention has been to provide the external of above-mentioned treatment burn and scald to give birth to the medical usage of skin oil.
In order to realize first purpose, the present invention has taked following technical measures:
Skin oil is given birth in a kind of external for the treatment of burn and scald, it is characterized in that being prepared from by the raw material of following weight portion proportioning:
Radix Scutellariae 15 ~ 25 weight portions, Radix Et Rhizoma Rhei 15 ~ 25 weight portions, the Radix Astragali 15 ~ 25 weight portions, Rhizoma Coptidis 15 ~ 25 weight portions, Cortex Phellodendri 15 ~ 25 weight portions, Radix Angelicae Sinensis 10 ~ 15 weight portions, Radix Sanguisorbae 10 ~ 15 weight portions, Radix Arnebiae (Radix Lithospermi) 10 ~ 15 weight portions, Radix Rehmanniae 6 ~ 10 weight portions, the Radix Angelicae Dahuricae 6 ~ 10 weight portions, Radix Cynanchi Atrati 6 ~ 10 weight portions, the Pseudobulbus Bletillae (Rhizoma Bletillae) 6 ~ 10 weight portions, Olibanum 10 ~ 15 weight portions, Myrrha 10 ~ 15 weight portions, Borneolum Syntheticum 3 ~ 8 weight portions, Oleum Sesami 800-1000 weight portion.
In order to realize second purpose, the present invention has taked following technical measures:
The preparation method of skin oil is given birth in a kind of external for the treatment of burn and scald of above-mentioned prescription, and its step is as follows:
Oleum Sesami is inserted in the container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, Cortex Phellodendri, Radix Angelicae Sinensis, Radix Sanguisorbae, Radix Arnebiae (Radix Lithospermi), Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, each medicinal powder of the Pseudobulbus Bletillae (Rhizoma Bletillae), stir;
Soak 3-10 after day, agitating heating, keeping oil temperature is 55-65 ℃, 1-2 hour turning off stove behind the filter and remove residue, adds Olibanum and Myrrh, 55-65 ℃ was stirred 5-30 minute, from fire, filter and remove residue is when treating that oil temperature is down to 30-40 ℃, add Borneolum Syntheticum, fully stir evenly, be cooled to room temperature, bottled rear sealed storage.
In order to realize the 3rd purpose, the present invention has taked following technical measures:
The basic process that the use of skin oil is given birth in external of the present invention is: clean wound surface with normal saline first, coating 0.1～1 millimeters thick, then monolayer sterile gauze flap coverage before using for the first time.After hindering front 4 day every day each coating 1 time when 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time when 8:00,13:00,18:00, totally 3 times, until wound healing.
Compared with prior art, the present invention has the following advantages and effect:
1, the present invention has scientifically made up 15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum and has been dipped in the living skin oil of Oleum Sesami preparation, and is simple to operate, easy to implement.
2. 15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum being dipped in Oleum Sesami scientifically combines, be monarch, minister, help, make the medicine combined effect to repair I～III degree empyrosis wound surface, more effective when using simultaneously than one or more medicines.Experimental result shows that the living skin oil among the present invention has following advantage to the treatment of I～III degree empyrosis wound surface; 1. the medicine cost is low, easy to use; 2. anti-infection ability is strong; 3. wound healing time short (I～II degree burn and scald needed to fully recover in 10～12 days clinically, and rabbit III degree is scalded needed to fully recover in 20～25 days), cicatrix is small (scalded after 22 days, and the wound surface area is by 6cm by rabbit III degree after the healing
2Be reduced to 0.1cm
2); 4. the smooth and smooth pliable of skin behind the wound healing, the new piliation in wound surface place is normal; The HE coloration result of wound tissue shows that the wound surface epidermis is complete after the healing, and skin corium has fine and close hair follicle.
3. burn and scald mechanism is not clear to affect it in the present situation of clinical expansion because treating based on present compound Chinese medicinal preparation, and the mechanism that the present invention treats III degree burn and scald to the living skin oil that is dipped in the Oleum Sesami preparation by 15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum is studied.Experimental result shows that giving birth to skin oil is by rise VEGF, FGF2, and TGF β 1 protein expression such as grade promotes fibroblast and epithelial cell proliferation, induces new vessels to form, and accelerates wound healing and scar repairing.
Description of drawings
Fig. 1 gives birth to skin oil 2 and promotes rabbit III degree empyrosis wound surface healing (gross examination of skeletal muscle)
Scalded rear the 1st day, it is obviously red and swollen that Oleum Sesami group and mupirocin are formed surface skin; Living skin line of oils wound surface part slightly has ruddy, and swelling is not obvious.Scalded rear the 11st day, the Stiff skin that Oleum Sesami is formed face comes off, and exposes subcutaneous Musclar layer; Mupirocin is formed the edge projection, the wound surface stiff skin; Living skin line of oils wound surface is partly formed a scab and is begun to come off, and new skin grows.Scalded rear the 22nd day, Oleum Sesami is formed face and is slightly caved in, and the wound surface place has no hair growth; Mupirocin is formed face incrustation part and is come off, and wound surface is smooth, has no hair growth; Almost all come off and give birth to the incrustation of skin line of oils wound surface, wound surface is clean, and there is newborn tiny hair at the wound surface place.The last scalding the 45th day, it is slightly hard that Oleum Sesami group, mupirocin are formed edge skin, wound surface skin depressions and have no hair growth, and cicatrix is obvious; And living skin line of oils wound surface is smooth smooth, and the epithelium hair growth is neat.Annotate: black arrow represents to scald 45 days and respectively organizes afterwards the rabbit wound surface.Scale is 1cm.
Fig. 2 gives birth to skin oil 2 anti-infection abilities strong (wound surface observation)
The last scalding the 13rd day, 6 wound surface of 3 rabbit of Oleum Sesami group all infected, and wherein 2 rabbit respectively have 1 traumatic infection serious, with purulent secretion, edge of wound red swelling of the skin, erosion, oozed out yellow liquid, sewed up after the debridement treatment; It is clean that mupirocin is formed face, edge of wound projection, wound surface stiff skin and coming off on a small quantity; Give birth to the incrustation of skin line of oils wound surface, the part incrustation comes off, and new skin grows, and healing is good.Scale is 1cm.
Fig. 3 gives birth to skin oil 3 and promotes skin regeneration, accelerates scar repairing (the HE dyeing of wound surface skin histology)
Oleum Sesami group and mupirocin are formed face width and dark, and epidermis thickens, and subcutaneously have no new saccus setigerus, and cicatrization is to Musclar layer; It is complete to give birth to skin line of oils wound surface skin texture, and subcutaneous visible newborn fine and close hair follicle has no clear scar and forms.Annotate: the black arrow mark is respectively organized rabbit skin wound section.Above-mentioned picture is the Automatic Combined of continuously shot images under 20 times of object lens of Nikon TiE electric microscope.Scale is 1mm.
Fig. 4 gives birth to skin oil 4 and raises FGF-2, the protein expressions (immunofluorescence dyeing) such as TGF-β 1, VEGF in the rabbit wound surface skin
Compare with the mupirocin group with the Oleum Sesami group, give birth to the protein expression showed increased such as FGF2, TGF-β 1 and VEGF in the skin line of oils rabbit wound surface skin.The picture of taking under 20 times of object lens of electronic fluorescence microscope, its scale are 200 μ m.
The specific embodiment
Following applicant is described in further detail product of the present invention and its preparation method and application in connection with specific embodiment, should be understood that following content is limited protection scope of the present invention never in any form.
Embodiment 1:
The preparation method of skin oil is given birth in a kind of external for the treatment of burn and scald, and its step is as follows:
The 800g Oleum sesami is inserted in the container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, each 15g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, each 10g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, each 6g of common bletilla stir;
Soak after 3 days, agitating heating, keeping oil temperature is 55 ℃, 2 hours turning off stove behind the filter and remove residue, add Olibanum and each 10g of Myrrh, 55 ℃ of agitating heating 30 minutes, from fire, filter and remove residue, when treating that oil temperature is down to 30 ℃, add Borneolum Syntheticum 3g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage gets living skin oil 1.
Embodiment 2:
The preparation method of skin oil is given birth in a kind of external for the treatment of burn and scald, and its step is as follows:
The 900g Oleum sesami is inserted in the container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, each 25g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, each 15g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, each 10g of common bletilla stir;
Soak after 10 days, agitating heating, keeping oil temperature is 65 ℃, 1 hour turning off stove behind the filter and remove residue, adds Olibanum and each 15g of Myrrh, 65 ℃ of agitating heating 20 minutes, from fire, filter and remove residue, when treating that oil temperature is down to 40 ℃, add Borneolum Syntheticum 8g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage gets living skin oil 2.
Embodiment 3:
The preparation method of skin oil is given birth in a kind of external for the treatment of burn and scald, and its step is as follows:
The 1000g Oleum sesami is inserted in the container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, each 20g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, each 12g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, each 8g of common bletilla stir;
Soak after 5 days, agitating heating, keeping oil temperature is 60 ℃, 1.5 hour turning off stove behind the filter and remove residue, adds Olibanum and each 12g of Myrrh, 60 ℃ of agitating heating 15 minutes, from fire, filter and remove residue, when treating that oil temperature is down to 35 ℃, add Borneolum Syntheticum 6g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage gets living skin oil 3.
Embodiment 4:
The preparation method of skin oil is given birth in a kind of external for the treatment of burn and scald, and its step is as follows:
The 1600g Oleum sesami is inserted in the container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, each 40g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, each 25g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, each 18g of common bletilla stir;
Soak after 5 days, agitating heating, keeping oil temperature is 60 ℃, 2 hours turning off stove behind the filter and remove residue, add Olibanum and each 25g of Myrrh, 60 ℃ of agitating heating 10 minutes, from fire, filter and remove residue, when treating that oil temperature is down to 35 ℃, add Borneolum Syntheticum 10g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage gets living skin oil 4.
Embodiment 5:
The preparation method of skin oil is given birth in a kind of external for the treatment of burn and scald, and its step is as follows:
The 2500g Oleum sesami is inserted in the container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, each 65g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, each 40g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, each 25g of common bletilla stir;
Soak after 8 days, agitating heating, keeping oil temperature is 62 ℃, 1 hour turning off stove behind the filter and remove residue, adds Olibanum and each 35g of Myrrh, 62 ℃ of agitating heating 5 minutes, from fire, filter and remove residue, when treating that oil temperature is down to 32 ℃, add Borneolum Syntheticum 20g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage gets living skin oil 5.
The basic process that the livings skin oil of above-described embodiment 1-5 preparation is used in treatment I～III degree burn and scald is: clean wound surface with normal saline first before the use for the first time, coating is in the wound surface place, monolayer sterile gauze flap coverage, repeat administration behind the certain hour of interval.The applicant has carried out the serial experiment checking to giving birth to skin oil in the application of preparation treatment I～III degree burn and scald medicament, think that said preparation can effectively prevent I～III degree scald wound to hinder postoperative infection, obviously shorten wound healing time, improve the wound healing percentage rate, reduce behind the wound healing cicatrix or almost without cicatrix.Said preparation can also raise FGF-2, the protein expression levels such as TGF-β 1, VEGF in the III degree Scald Burn Rabbits wound surface skin, promotes fibroblast and epithelial cell proliferation, induces new vessels to form, thereby accelerates skin regeneration and scar repairing.
Embodiment 6 clinical observations: give birth to skin oil and promote I～II degree empyrosis wound surface healing:
The living skin oil of the present invention's preparation can be accelerated I～II degree empyrosis wound surface healing clinically.
1. physical data
Adopt living skin oil of the present invention that 30 routine burned patients are carried out clinical observation treatment, male's 16 examples wherein, women's 14 examples; Oldest 68 years old, minimum 3 years old, 36 years old mean age; Area 2%-22%, average area are 12.8%, all without shock symptom; The burn and scald number of degrees are I degree, light degree Ⅱ, the II degree all has deeply, take dark II degree as maximum; The position is from women's head-ornaments, trunk and extremity, and each position all has, take the limbs position as maximum.
2. Therapeutic Method
30 routine patients all adopt living skin oil of the present invention (the livings skin oily 1 that is made by embodiment 1 or the living skin oil 5 that is made by embodiment 5, each 15 example) treatment by its Therapeutic Method, is cleaned scald wound with normal saline first before facing medication, coating 0.1～1 millimeters thick, then monolayer sterile gauze flap coverage.Hinder in rear 4 days the every day of each coating 1 time when 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time when 8:00,13:00,18:00, totally 3 times, until wound healing.
3. wound surface is observed
The wound surface crust all comes off in succession after medication 3-5 days, and the rear wound surface that comes off is canescence, and wound surface transfers cerise to after 4-8 days.The patient feels that all the wound surface place is nice and cool after the medication, and pain disappears, and the medication phase is without any discomfort, when the child changes dressings also without the phenomenon of crying and screaming.Do not find traumatic infection in the medication process, wound surface healing in average 11 days do not stay residual wound after the healing, and there is hair growth at the wound surface place.
Embodiment 7 gives birth to skin oil and promotes rabbit III degree wound tissue healing:
The living skin oil of the present invention's preparation shortens the healing time of rabbit III degree scald wound.
Animal
9 of Japan's health large ear rabbits, the male and female dual-purpose, body weight 2.2～2.5Kg is provided by Wuhan Biological Products Inst., animal licence numbering SCXK(Hubei Province) 2008-0003.The single cage of rabbit is raised, and free drinking water diet is raised room temperature and remained on 25 ± 1 ℃, and the light and shade cycle is 12 hours.
1. the preparation of rabbit III degree scalding model and administration
After rabbit conformed a week, lumbar injection 0.3% sodium phenobarbital (15ml/kg) was with its anesthesia, and with shaving a mao machine (CP-7800, Shenzhen Coudé scholar) with its back depilation, area is 5cm * 5cm.Then with electromagnetic oven that water in the kettle is boiled and keep the state of constantly boiling, Hu Kou to be linked 50ml by a Heat insulated conduit remove mouth at the bottom of the end centrifuge tube, (area is 6cm to the centrifugal mouth of pipe
2) closely press and touch at tested rabbit back (every rabbit 2 place's wound surface, the left back of the body, the right back of the body be 1 place respectively) skin of unhairing place, each steam scalds to press and continues 30 seconds, sets up III degree scalding model (pathology through this experiment scald wound skin histology detect confirmation).
Scald Burn Rabbits adopts the table of random number method to be divided into three groups, comprise the Oleum Sesami group, give birth to skin line of oils and mupirocin group, 3 every group, and smear respectively Oleum Sesami, give birth to skin oil (the living skin oil 2 that is made by embodiment 2) and mupirocin ointment, monolayer sterile gauze flap coverage.Hinder in rear 4 days the every day of each coating 1 time when 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time when 8:00,13:00,18:00, totally 3 times, until wound healing.
2. rabbit III degree scald wound gross examination of skeletal muscle
The wound surface picture is observed and taken to postoperative every day, and the record wound surface changes, and goes out the wound healing area by Nikon NIS-element AR 3.1 computed in software again, healing percentage rate and healing time.
3. experimental result
The wound surface gross examination of skeletal muscle is seen Fig. 1 and Fig. 2, and wound healing time and wound healing area percentage see Table 1, scalds rear the 1st day, and it is obviously red and swollen that Oleum Sesami group and mupirocin are formed surface skin; Living skin line of oils wound surface part slightly has ruddy, and swelling is not obvious.Scalded rear the 11st day, and gave birth to the incrustation of skin line of oils wound surface parts of skin and begin to come off, new skin grows.Scalded rear the 13rd day, infecting all appears in 6 wound surface of 3 rabbit of Oleum Sesami group, and wherein 2 rabbit respectively have 1 traumatic infection serious, with purulent secretion, edge of wound red swelling of the skin, erosion, ooze out yellow liquid, sew up after the debridement treatment, rear continuation is smeared processing with Oleum Sesami; Give birth to the almost all incrustations of skin line of oils wound surface this moment, the part incrustation comes off, and new skin grows.Scalded rear the 22nd day, and gave birth to the incrustation of skin line of oils and almost all come off, wound surface is clean, and the wound surface area is by 6cm
2Be reduced to 0.1cm
2Scalded rear the 45th day, living skin line of oils wound surface is smooth smooth, and the epithelium hair growth is normal.
Table 1 is given birth to skin oil 2 and is shortened the healing time of rabbit III degree scald wound and improve the wound healing area percentage
F
1, P
1Be worth for a period of time, phase point compared gained, F together between group
2, P
2Value in the group each the time phase point gained relatively; Compare with the Oleum Sesami group,
aP＜0.05; Compare with the mupirocin group,
bP＜0.05; With in the group when last phase point relatively,
cP＜0.05.Compare with the Oleum Sesami group, give birth to skin line of oils wound healing time and obviously shorten (P＜0.05); In 5～22 days, give birth to skin line of oils wound tissue healing percentage ratio apparently higher than Oleum Sesami group (P＜0.05) after scalding.
Embodiment 8 gives birth to skin oil and promotes the wound surface skin regeneration, accelerates scar repairing:
Animal
Japan's health large ear rabbit, 9, the male and female dual-purpose, body weight 2.2～2.5Kg is by providing from Wuhan Biological Products Inst..The single cage of rabbit is raised, and free drinking water diet is raised room temperature and remained on 25 ± 1 ℃, and the light and shade cycle is 12 hours.
1. the preparation of rabbit III degree scalding model and grouping administration
After rabbit conformed a week, lumbar injection 0.3% sodium phenobarbital (15ml/kg) was with its anesthesia, and with shaving a mao machine (CP-7800, Shenzhen Coudé scholar) with its back depilation, area is 5cm * 5cm.Then with electromagnetic oven that water in the kettle is boiled and keep the state of constantly boiling, Hu Kou to be linked 50ml by a Heat insulated conduit remove mouth at the bottom of the end centrifuge tube, (area is 6cm to the centrifugal mouth of pipe
2) closely press and touch at tested rabbit back (every rabbit 2 place's wound surface, the left back of the body, the right back of the body be 1 place respectively) skin of unhairing place, each steam scalds to press and continues 30 seconds, sets up III degree scalding model (pathology through this experiment scald wound skin histology detect confirmation).
Scald Burn Rabbits employing table of random number method is divided into three groups and comprises the Oleum Sesami group, gives birth to skin line of oils and mupirocin group, 3 every group, 2 wound surface of every rabbit, and smear respectively Oleum Sesami, give birth to skin oil (the living skin oil 3 that is made by embodiment 3) and mupirocin ointment, monolayer sterile gauze flap coverage.Hinder in rear 4 days the every day of each coating 1 time when 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time when 8:00,13:00,18:00, totally 3 times, until wound healing.
2. rabbit III degree scald wound pathological study
Rabbit was scalded after 45 days, the processing of discontinuing medication.After the air tap inserting method is put to death rabbit, get and respectively organize the skin sample (1cm * 3cm) be fixed in 10% formalin of rabbit edge of wound place.After 24 hours, with tap water rinsing 3 hours, then gradient ethanol dehydration, routine paraffin wax embedding, section (thickness is 4 μ m), carry out HE dyeing.
3. experimental result
Scald after 45 days, the HE coloration result of wound tissue as shown in Figure 3, Oleum Sesami group and mupirocin are formed face width and dark, epidermis thickens, and subcutaneously has no new saccus setigerus, cicatrization is to Musclar layer; Compare with front two groups, living skin line of oils scald wound skin texture is complete, the hair follicle of the i.e. new life's densification of the appendages of skin that subcutaneous visible growth is complete, and newborn arrangement of collagen fibers is neat, compares no significant difference with normal skin tissue.
Embodiment 9 gives birth to skin oil and raises FGF-2, the protein expressions such as TGF-β 1, VEGF in the rabbit wound surface skin:
Because the pharmacological Mechanism for the treatment of burn and scald is not clear, make its present situation that can't apply clinically based at present a lot of compound Chinese medicinal preparation, the mechanism that the present invention scalds living skin oil treatment III degree is studied.It is reported that the empyrosis wound surface reparation is regulated and control by many factors, wherein abundant nutrient substance and oxygen supply is the basis of wound repair, the wound surface Microembolization makes the tissue ischemia anoxia behind the burn and scald, it is one of major reason that causes the early stage intensification of wound surface and necrosis, can the microvascular reconstruction of wound surface then is related to repair tissue obtain sufficient blood flow and oxygen confession, help wound surface quickly-healing (Comparison of oxidative stress biomarkerprofiles between acute and chronic wound environments.Wound Repair Regen.2004,12:419-429).Vascular endothelial cell growth factor (VEGF) is single-minded mitosis stimulating factor and chemotactic factor of endotheliocyte, and scalable vascular endothelial cell proliferation, migration increase microvascular permeability, accelerates the formation of new vessels; FGF2 (FGF-2) is the very strong protein factor of a kind of Promote cell's growth effect, can stimulate propagation and the migration of capillary endothelial cell, lure into blood vessel endothelium grow at the bottom of the gum base in and form and the similar tube chamber spline structure of blood capillary, thereby progressively produce new vessels; Transforminggrowthfactor-β1 (TGF β 1) is the at present known somatomedin the closest and the most representative with cicatrization, and it can promote the biosynthesis of vegf protein in fibroblast and vascular endothelial cell.
Animal
Japan's health large ear rabbit, 9, the male and female dual-purpose, body weight 2.2～2.5Kg is provided by Wuhan Biological Products Inst..The single cage of rabbit is raised, and free drinking water diet is raised room temperature and remained on 25 ± 1 ℃, and the light and shade cycle is 12 hours.
1. the preparation of rabbit III degree scalding model and grouping administration
After rabbit conformed a week, lumbar injection 0.3% sodium phenobarbital (15ml/kg) was with its anesthesia, and with shaving a mao machine (CP-7800, Shenzhen Coudé scholar) with its back depilation, area is 5cm * 5cm.Then with electromagnetic oven that water in the kettle is boiled and keep the state of constantly boiling, Hu Kou to be linked 50ml by a Heat insulated conduit remove mouth at the bottom of the end centrifuge tube, (area is 6cm to the centrifugal mouth of pipe
2) closely press and touch at tested rabbit back (every rabbit 2 place's wound surface, the left back of the body, the right back of the body be 1 place respectively) skin of unhairing place, each steam scalds to press and continues 30 seconds, sets up III degree scalding model (pathology through this experiment scald wound skin histology detect confirmation).
Scald Burn Rabbits employing table of random number method is divided into three groups and comprises the Oleum Sesami group, gives birth to skin line of oils and mupirocin group, 3 every group, 2 wound surface of every rabbit, and smear respectively Oleum Sesami, give birth to skin oil (the living skin oil 4 that is made by embodiment 4) and mupirocin ointment, monolayer gauze flap coverage.After hindering front 4 interior every days each coating 1 time when 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time when 8:00,13:00,18:00, totally 3 times, until wound healing.
2. rabbit III degree scald wound histogenic immunity Fluorescent Staining Observation
Rabbit was scalded after 45 days, the processing of discontinuing medication.After the air tap inserting method is put to death rabbit, get and respectively organize the skin sample (1cm * 3cm) be fixed in 10% formalin of rabbit edge of wound place.After 24 hours, with tap water rinsing 3 hours, then gradient ethanol dehydration, routine paraffin wax embedding, section (thickness is 4 μ m), carry out the immunofluorescence dyeing of VEGF, TGF β 1, FGF2.Immunofluorescence dyeing adopts lowlenthal serum sealing 30 minutes, then removes confining liquid, adds respectively TGF β 1, FGF2, and three kinds of polyclonal antibodies of VEGF (three kinds of antibody is all with the dilution proportion of 1:100), juxtaposition is cut into slices and is spent the night in 4 ℃ of refrigerator moisture keepings.PBS washes and adds afterwards Alxa Fluor 488 goat-anti rabbit IGg(for three times with 1: 1000 dilution proportion), room temperature moisturizing and lucifuge were hatched 1 hour; At last, PBS washes three sections, mounting, and (Nikon Eclipse Ti, Japan) takes pictures under the electronic fluorescence microscope.Estimate living skin oil to the ability of wound surface skin regeneration and reparation by the expression of the above-mentioned three kinds of cell growth factor of NikonNIS-element AR 3.1 software analysis.
3. experimental result
Adopt the scald of the method detection of immunofluorescence dyeing respectively to organize afterwards the TGF-β of rabbit wound surface place 1 in 45 days, FGF2, the protein expression situations such as VEGF are compared with the mupirocin group with the Oleum Sesami group as shown in Figure 4, and the visible FGF2 protein expression of living skin line of oils wound surface skin is higher.It is less that Oleum Sesami group and mupirocin are formed in the surface skin TGF-β 1 protein expression; Visible TGF-β 1 protein expression is obvious in the living skin line of oils wound surface skin.Vegf protein Oleum Sesami group and mupirocin form express in the surface skin less; Compare with front two groups, give birth to the visible vegf protein of skin line of oils wound surface skin and express obviously increase.
Claims (6)
1. skin oil is given birth in an external for the treatment of burn and scald, it is characterized in that being prepared from by following raw material:
Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, Cortex Phellodendri, Radix Angelicae Sinensis, Radix Sanguisorbae, Radix Arnebiae (Radix Lithospermi), Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Olibanum, Myrrha, Borneolum Syntheticum and Oleum Sesami.
2. skin oil is given birth in external as claimed in claim 1, it is characterized in that being prepared from by the raw material of following weight portion proportioning:
Radix Scutellariae 15 ~ 25 weight portions, Radix Et Rhizoma Rhei 15 ~ 25 weight portions, the Radix Astragali 15 ~ 25 weight portions, Rhizoma Coptidis 15 ~ 25 weight portions, Cortex Phellodendri 15 ~ 25 weight portions, Radix Angelicae Sinensis 10 ~ 15 weight portions, Radix Sanguisorbae 10 ~ 15 weight portions, Radix Arnebiae (Radix Lithospermi) 10 ~ 15 weight portions, Radix Rehmanniae 6 ~ 10 weight portions, the Radix Angelicae Dahuricae 6 ~ 10 weight portions, Radix Cynanchi Atrati 6 ~ 10 weight portions, the Pseudobulbus Bletillae (Rhizoma Bletillae) 6 ~ 10 weight portions, Olibanum 10 ~ 15 weight portions, Myrrha 10 ~ 15 weight portions, Borneolum Syntheticum 3 ~ 8 weight portions, Oleum Sesami 800-1000 weight portion.
3. the preparation method of skin oil is given birth in a claim 1 or 2 described externals, and its step is as follows:
Oleum Sesami is inserted in the container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, Cortex Phellodendri, Radix Angelicae Sinensis, Radix Sanguisorbae, Radix Arnebiae (Radix Lithospermi), Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, each medicinal powder of the Pseudobulbus Bletillae (Rhizoma Bletillae), stir;
Soak 3-10 after day, agitating heating, keeping oil temperature is 55-65 ℃, 1-2 hour turning off stove behind the filter and remove residue, adds Olibanum and Myrrh, 55-65 ℃ was stirred 5-30 minute, from fire, filter and remove residue is when treating that oil temperature is down to 30-40 ℃, add Borneolum Syntheticum, fully stir evenly, be cooled to room temperature, bottled rear sealed storage.
4. the medical usage of skin oil is given birth in arbitrary described external among the claim 1-3.
5. the application of skin oil in the preparation burn and scald medicine given birth in arbitrary described external among the claim 1-3.
6. the application of skin oil in the medical apparatus and instruments for the treatment of for the preparation of burn and scald given birth in arbitrary described external among the claim 1-3.
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Effective date of registration: 20160624 Address after: 518000 Guangdong city of Shenzhen province Futian District Shatou street Che Kung Temple Tairan six road cedar building B, 9A Patentee after: Shenzhen origin Biotechnology Co., Ltd. Address before: 430074 School of pharmacy, South-Central University For Nationalities, 708 National Road, Hongshan District, Hubei, Wuhan Patentee before: Tang Hebin |