CN102895526B - Externally-applied skin-generating oil for treatment of burn and preparation method and application thereof - Google Patents
Externally-applied skin-generating oil for treatment of burn and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of a traditional Chinese medicine preparation, and particularly discloses externally-applied skin-generating oil for treatment of burns and a preparation method and application thereof. The skin-generating oil is prepared by soaking the following 15 Chinese herbs in sesame oil: radix scutellariae, rheum officinale, astragalus mongholicus, coptis chinensis, cortex phellodendri, angelica sinensis, sanguisorba officinalis, lithospermum, rehmannia, radix angelicae, cynanchum atratum, bletilla striata, olibanum, myrrh and borneol. When the skin-generating oil is applied in treatment of burns, wound infection caused after injury can be prevented effectively, proliferation and migration of fibroblasts and vascular endothelial cells can be promoted, neovascularization can be induced, wound healing can be accelerated, scar repair can be promoted, the wound healing time can be shortened, Class I-II burns can be cured in 10-12 days, and Class III burns of rabbits can be cured in 20-25 days. When Class III burns of rabbits are treated with the skin-generating oil, the wound area can be reduced from 6 cm<2> to 0.1cm<2>, the wound surface is flat, smooth and soft, and newborn hair is normal. The wound epidermis can be recovered completely, dense hair follicles can be formed in the dermis layer, and almost no difference from normal skin tissues can be produced.
Description
Technical field
The present invention relates to Chinese medicine preparation technical field, relate in particular to a kind of raw skin oil of external for the treatment of burn and scald and its preparation method and application.
Background technology
China's burn and scald Annual occurence rate accounts for 0.5%～1.0% of total population at present according to statistics, and burn and scald disease is a kind of somatic damage mainly being caused by Gao Re or calcination.According to the degree of burn and scald, burn and scald generally can be divided into I degree, shallow II degree, dark II degree and III degree burn and scald.Wherein I degree and II degree burn and scald are relatively easily controlled and treatment clinically, and III degree burn and scald is the damage of skin holostrome, what have even injures subcutaneous tissue, muscle and skeleton etc., after wound, wound surface easily infects, after healing, often form cicatrix in various degree, sometimes also there will be scar hyperplasia and muscle contraction.According to statistics, the disability rate of China's large-area burns reaches 48% at present, and wherein between twenty and fifty disability rate is about 78.1%(burn wound therapeutic strategy. China burn magazine .2011,27:247-249).Burn and scald grievous injury China people's physical and mental health and economic interests.Wherein the treatment reparation of III degree empyrosis wound surface skin, becomes one of current problem demanding prompt solution especially.
At present adopt the dosage form such as ointment of doctor trained in Western medicine to treat to the treatment of I～II degree burn and scald clinically more, but this therapeutic modality exists certain side effect, there is the thorough consequently recurrence for the treatment of and problem (Chinese patent application 201010516285.6) repeatedly in some wound surface; Meanwhile, adopt dermatoplastic method clinically, but cost is higher to the treatment of III degree burn and scald is normal, treatment time is longer.On present stage market, effectively the Chinese medicine preparation for the treatment of III degree burn and scald is relatively deficient, most of medicine Qinghuo Jiedu in time after use, and alleviating pain and detumescence, easily stays cicatrix after wound healing, still need to do cosmetic surgery in the future.The Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum are usually used in treating burn and scald, but one or several is used for the treatment of I degree and II degree burn and scald while using mostly these medicine lists, be rarely used in III degree burn and scald treatment (China burn is complete works of. Beijing: the .1998:234-257 of publishing house of institute).Also 15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum are not made to preparation at present, for the preparation of the relevant report for the treatment of III degree burn and scald medicament.
Summary of the invention
For the deficiencies in the prior art, first object of the present invention has been to provide a kind of raw skin oil of external for the treatment of burn and scald.
Second object of the present invention has been to provide a kind of preparation method of the raw skin oil of external of above-mentioned treatment burn and scald.
The 3rd object of the present invention has been to provide the medical usage of the raw skin oil of external of above-mentioned treatment burn and scald.
In order to realize first object, the present invention has taked following technical measures:
The raw skin oil of external for the treatment of burn and scald, is characterized in that being prepared from by the raw material of following weight portion proportioning:
Radix Scutellariae 15 ~ 25 weight portions, Radix Et Rhizoma Rhei 15 ~ 25 weight portions, the Radix Astragali 15 ~ 25 weight portions, Rhizoma Coptidis 15 ~ 25 weight portions, Cortex Phellodendri 15 ~ 25 weight portions, Radix Angelicae Sinensis 10 ~ 15 weight portions, Radix Sanguisorbae 10 ~ 15 weight portions, Radix Arnebiae (Radix Lithospermi) 10 ~ 15 weight portions, Radix Rehmanniae 6 ~ 10 weight portions, the Radix Angelicae Dahuricae 6 ~ 10 weight portions, Radix Cynanchi Atrati 6 ~ 10 weight portions, the Pseudobulbus Bletillae (Rhizoma Bletillae) 6 ~ 10 weight portions, Olibanum 10 ~ 15 weight portions, Myrrha 10 ~ 15 weight portions, Borneolum Syntheticum 3 ~ 8 weight portions, Oleum Sesami 800-1000 weight portion.
In order to realize second object, the present invention has taked following technical measures:
A preparation method for the raw skin oil of external of the treatment burn and scald of above-mentioned formula, its step is as follows:
Oleum Sesami is inserted in container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, Cortex Phellodendri, Radix Angelicae Sinensis, Radix Sanguisorbae, Radix Arnebiae (Radix Lithospermi), Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the each medicinal powder of the Pseudobulbus Bletillae (Rhizoma Bletillae), stir;
Soak after 3-10 day, agitating heating, maintenance oil temperature is 55-65 ℃, 1-2 hour turning off stove, after filter and remove residue, adds Olibanum and Myrrh, 55-65 ℃ is stirred 5-30 minute, from fire, filter and remove residue, in the time that oil temperature is down to 30-40 ℃, add Borneolum Syntheticum, fully stir evenly, be cooled to room temperature, bottled rear sealed storage.
In order to realize the 3rd object, the present invention has taked following technical measures:
The basic process that the raw skin oil of external of the present invention uses is: before using for the first time, first clean wound surface with normal saline, coating 0.1～1 millimeters thick, then monolayer sterile gauze flap coverage.After wound first 4 day every day each coating 1 time in the time of 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time in the time of 8:00,13:00,18:00, totally 3 times, until wound healing.
Compared with prior art, the present invention has the following advantages and effect:
1, the present invention has scientifically combined 15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum and has been dipped in the raw skin oil of Oleum Sesami preparation, simple to operate, easy to implement.
2. 15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum being dipped in to Oleum Sesami scientifically combines, be monarch, minister, help, make medicine combined effect to repair I～III degree empyrosis wound surface, more effective while use than one or more medicines simultaneously.Experimental result shows that the raw skin oil in the present invention has following advantage to the treatment of I～III degree empyrosis wound surface; 1. medicine cost is low, easy to use; 2. anti-infection ability is strong; 3. wound healing time short (I～II degree burn and scald needs can fully recover for 10～12 days clinically, and rabbit III degree is scalded to be needed can fully recover for 20～25 days), the rear cicatrix of healing is small, and (rabbit III degree was scalded after 22 days, and wound surface area is by 6cm
2be reduced to 0.1cm
2); 4. the smooth and smooth pliable of skin after wound healing, the new piliation in wound surface place is normal; The HE coloration result demonstration of wound tissue, after healing, wound surface epidermis is complete, and skin corium has fine and close hair follicle.
Based on current compound Chinese medicinal preparation because of not clear its present situation at clinical expansion that affects for the treatment of burn and scald mechanism, the mechanism that the present invention treats III degree burn and scald to the raw skin oil that is dipped in Oleum Sesami by 15 kinds of Chinese medicines such as Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Cortex Phellodendri, Rhizoma Coptidis, Radix Sanguisorbae, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Angelicae Sinensis, Radix Arnebiae (Radix Lithospermi), Olibanum, Myrrha, Borneolum Syntheticum and prepares is studied.Experimental result shows, raw skin oil is that TGF β 1 protein expression such as grade, promotes fibroblast and epithelial cell proliferation by raising VEGF, FGF2, and induction new vessels forms, and accelerates wound healing and scar repairing.
Accompanying drawing explanation
Fig. 1 is raw, and skin oil 2 promotes rabbit III degree empyrosis wound surface healing (gross examination of skeletal muscle)
Scald latter the 1st day, it is obviously red and swollen that Oleum Sesami group and mupirocin are formed surface skin; Raw skin line of oils wound surface part is slightly ruddy, and swelling is not obvious.Scald latter the 11st day, the Stiff skin that Oleum Sesami is formed face comes off, and exposes subcutaneous Musclar layer; Mupirocin is formed edge projection, wound surface stiff skin; The incrustation of raw skin line of oils wound surface part starts to come off, and new skin grows.Scald latter the 22nd day, Oleum Sesami is formed face and is slightly caved in, and wound surface place has no hair growth; Mupirocin is formed face incrustation part and is come off, and wound surface is smooth, has no hair growth; And the incrustation of raw skin line of oils wound surface almost all comes off, wound surface is clean, and there is newborn tiny hair at wound surface place.After scalding the 45th day, it is slightly hard that Oleum Sesami group, mupirocin are formed edge skin, wound surface skin depressions and have no hair growth, and cicatrix is obvious; And raw skin line of oils wound surface is smooth, epithelium hair growth is neat.Note: black arrow represents to scald 45 days and respectively organizes afterwards rabbit wound surface.Scale is 1cm.
Fig. 2 raw skin oil 2 anti-infection abilities strong (wound surface observation)
After scalding the 13rd day, 6 wound surface of 3 rabbit of Oleum Sesami group all infected, and wherein 2 rabbit respectively have 1 traumatic infection serious, with purulent secretion, edge of wound red swelling of the skin, erosion, ooze out yellow liquid, after debridement treatment, sew up; It is clean that mupirocin is formed face, edge of wound projection, wound surface stiff skin and coming off on a small quantity; The incrustation of raw skin line of oils wound surface, part incrustation comes off, and new skin grows, and healing is good.Scale is 1cm.
Fig. 3 is raw, and skin oil 3 promotes skin regeneration, accelerates scar repairing (the HE dyeing of wound surface skin histology)
Oleum Sesami group and mupirocin are formed face width and dark, and epidermis thickens, and subcutaneously have no new saccus setigerus, and cicatrization is to Musclar layer; Raw skin line of oils wound surface skin texture is complete, and subcutaneous visible newborn fine and close hair follicle, has no clear scar and form.Note: black arrow marks each group of rabbit skin wound section.Above-mentioned picture is the Automatic Combined of continuously shot images under 20 times of object lens of Nikon TiE electric microscope.Scale is 1mm.
The raw skin oil 4 of Fig. 4 raises in rabbit wound surface skins the protein expressions (immunofluorescence dyeing) such as FGF-2, TGF-β 1, VEGF
Compare with mupirocin group with Oleum Sesami group, give birth to the protein expression showed increased such as FGF2, TGF-β 1 and VEGF in skin line of oils rabbit wound surface skin.The picture of taking under 20 times of object lens of electronic fluorescence microscope, its scale is 200 μ m.
The specific embodiment
Applicant is described in further detail product of the present invention and its preparation method and application in connection with specific embodiment below, should be understood that following content is limited protection scope of the present invention never in any form.
Embodiment 1:
A preparation method for the treatment of the raw skin oil of external of burn and scald, its step is as follows:
800g Oleum sesami is inserted in container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, the each 15g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, the each 10g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the each 6g of common bletilla, stir;
Soak after 3 days, agitating heating, keeping oil temperature is 55 ℃, 2 hours turning off stove, after filter and remove residue, add the each 10g of Olibanum and Myrrh, 55 ℃ of agitating heating 30 minutes, from fire, filter and remove residue, in the time that oil temperature is down to 30 ℃, add Borneolum Syntheticum 3g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage, obtains raw skin oil 1.
Embodiment 2:
A preparation method for the treatment of the raw skin oil of external of burn and scald, its step is as follows:
900g Oleum sesami is inserted in container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, the each 25g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, the each 15g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the each 10g of common bletilla, stir;
Soak after 10 days, agitating heating, keeping oil temperature is 65 ℃, 1 hour turning off stove, after filter and remove residue, adds the each 15g of Olibanum and Myrrh, 65 ℃ of agitating heating 20 minutes, from fire, filter and remove residue, in the time that oil temperature is down to 40 ℃, add Borneolum Syntheticum 8g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage, obtains raw skin oil 2.
Embodiment 3:
A preparation method for the treatment of the raw skin oil of external of burn and scald, its step is as follows:
1000g Oleum sesami is inserted in container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, the each 20g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, the each 12g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the each 8g of common bletilla, stir;
Soak after 5 days, agitating heating, keeping oil temperature is 60 ℃, 1.5 hours turning off stove, after filter and remove residue, add the each 12g of Olibanum and Myrrh, 60 ℃ of agitating heating 15 minutes, from fire, filter and remove residue, in the time that oil temperature is down to 35 ℃, add Borneolum Syntheticum 6g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage, obtains raw skin oil 3.
Embodiment 4:
A preparation method for the treatment of the raw skin oil of external of burn and scald, its step is as follows:
1600g Oleum sesami is inserted in container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, the each 40g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, the each 25g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the each 18g of common bletilla, stir;
Soak after 5 days, agitating heating, keeping oil temperature is 60 ℃, 2 hours turning off stove, after filter and remove residue, add the each 25g of Olibanum and Myrrh, 60 ℃ of agitating heating 10 minutes, from fire, filter and remove residue, in the time that oil temperature is down to 35 ℃, add Borneolum Syntheticum 10g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage, obtains raw skin oil 4.
Embodiment 5:
A preparation method for the treatment of the raw skin oil of external of burn and scald, its step is as follows:
2500g Oleum sesami is inserted in container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, the each 65g of Cortex Phellodendri powder, Radix Angelicae Sinensis, Radix Sanguisorbae, the each 40g of Arnebia Root, Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the each 25g of common bletilla, stir;
Soak after 8 days, agitating heating, keeping oil temperature is 62 ℃, 1 hour turning off stove, after filter and remove residue, adds the each 35g of Olibanum and Myrrh, 62 ℃ of agitating heating 5 minutes, from fire, filter and remove residue, in the time that oil temperature is down to 32 ℃, add Borneolum Syntheticum 20g, fully stir evenly, be cooled to room temperature, bottled rear sealed storage, obtains raw skin oil 5.
The basic process that raw skin oil prepared by above-described embodiment 1-5 is applied in treatment I～III degree burn and scald is: before using for the first time, first clean wound surface with normal saline, coating is in wound surface place, monolayer sterile gauze flap coverage, repeat administration after the certain hour of interval.Applicant has carried out serial experiment checking to raw skin oil in the application of preparation treatment I～III degree burn and scald medicament, think that said preparation can effectively prevent I～III degree scald wound to hinder postoperative infection, obviously shorten wound healing time, improve wound healing percentage rate, reduce after wound healing cicatrix or almost without cicatrix.Said preparation can also raise the expression of the albumen such as FGF-2, TGF-β 1, VEGF in III degree Scald Burn Rabbits wound surface skin, promotes fibroblast and epithelial cell proliferation, and induction new vessels forms, thereby accelerates skin regeneration and scar repairing.
Embodiment 6 clinical observations: raw skin oil promotes the healing of I～II degree empyrosis wound surface:
Raw skin oil prepared by the present invention can be accelerated the healing of I～II degree empyrosis wound surface clinically.
1. physical data
Adopt raw skin oil of the present invention to carry out clinical observation treatment to 30 routine burned patients, wherein male's 16 examples, women's 14 examples; Oldest 68 years old, minimum 3 years old, 36 years old mean age; Area 2%-22%, average area is 12.8%, all without shock symptom; The burn and scald number of degrees are I degree, light degree Ⅱ, II degree all has deeply, take dark II degree as maximum; Position is from women's head-ornaments, trunk and extremity, and each position all has, take limbs position as maximum.
2. Therapeutic Method
30 routine patients all adopt raw skin oil of the present invention (the raw skin oil 1 being made by embodiment 1 or the raw skin oil 5 being made by embodiment 5, each 15 examples) treatment, by its Therapeutic Method, first clean scald wound with normal saline before facing medication, coating 0.1～1 millimeters thick, then monolayer sterile gauze flap coverage.Hinder in latter 4 days the every day of each coating 1 time in the time of 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time in the time of 8:00,13:00,18:00, totally 3 times, until wound healing.
3. wound surface is observed
After medication 3-5 days, wound surface crust all comes off in succession, and after coming off, wound surface is canescence, and after 4-8 days, wound surface transfers cerise to.After medication, patient all feels that wound surface place is nice and cool, and pain disappears, and the medication phase is without any discomfort, in the time that child changes dressings also without the phenomenon of crying and screaming.In medication process, do not find traumatic infection, wound surface healing in average 11 days, do not stay residual wound, and there is hair growth at wound surface place after healing.
The raw skin oil of embodiment 7 promotes rabbit III degree wound tissue healing:
Raw skin oil prepared by the present invention shortens the healing time of rabbit III degree scald wound.
Animal
9 of Japan's health large ear rabbits, male and female dual-purpose, body weight 2.2～2.5Kg, is provided by Wuhan Biological Products Inst., animal licence numbering SCXK(Hubei Province) 2008-0003.The single cage of rabbit is raised, and free drinking water diet is raised room temperature and remained on 25 ± 1 ℃, and the light and shade cycle is 12 hours.
1. the preparation of rabbit III degree scalding model and administration
Conform after one week until rabbit, lumbar injection 0.3% sodium phenobarbital (15ml/kg) is anaesthetized, and with shaving a mao machine (CP-7800, Shenzhen Coudé scholar) by its back depilation, area is 5cm × 5cm.Then with electromagnetic oven by boiled water in kettle and keep the state of constantly boiling, Hu Kou is linked to 50ml by a Heat insulated conduit and removes mouth at the bottom of end centrifuge tube, (area is 6cm to the centrifugal mouth of pipe
2) closely press and touch at tested rabbit back (every rabbit 2 place's wound surface, the left back of the body, each 1 place of the right back of the body) skin of unhairing place, each steam scalds to press and continues 30 seconds, sets up III degree scalding model (detect and confirm through the pathology of this experiment scald wound skin histology).
Scald Burn Rabbits adopts table of random number method to be divided into three groups, comprise Oleum Sesami group, raw skin line of oils and mupirocin group, 3 every group, and smear respectively Oleum Sesami, raw skin oil (the raw skin oil 2 being made by embodiment 2) and mupirocin ointment, monolayer sterile gauze flap coverage.Hinder in latter 4 days the every day of each coating 1 time in the time of 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time in the time of 8:00,13:00,18:00, totally 3 times, until wound healing.
2. rabbit III degree scald wound gross examination of skeletal muscle
Observe and take wound surface picture postoperative every day, records wound surface and change, then go out wound healing area by Nikon NIS-element AR 3.1 computed in software, healing percentage rate and healing time.
3. experimental result
Wound surface gross examination of skeletal muscle is shown in Fig. 1 and Fig. 2, and wound healing time and wound healing area percentage, in table 1, are scalded latter the 1st day, and it is obviously red and swollen that Oleum Sesami group and mupirocin are formed surface skin; Raw skin line of oils wound surface part is slightly ruddy, and swelling is not obvious.Scald latter the 11st day, the incrustation of raw skin line of oils wound surface parts of skin starts to come off, and new skin grows.Scald latter the 13rd day, infecting all appears in 6 wound surface of 3 rabbit of Oleum Sesami group, and wherein 2 rabbit respectively have 1 traumatic infection serious, with purulent secretion, edge of wound red swelling of the skin, erosion, ooze out yellow liquid, after debridement treatment, sew up, rear continuation is smeared processing with Oleum Sesami; Now almost all incrustations of raw skin line of oils wound surface, part incrustation comes off, and new skin grows.Scald latter the 22nd day, raw skin line of oils incrustation almost all comes off, and wound surface is clean, and wound surface area is by 6cm
2be reduced to 0.1cm
2.Scald latter the 45th day, raw skin line of oils wound surface is smooth, and epithelium hair growth is normal.
The raw skin oil 2 of table 1 shortens the healing time of rabbit III degree scald wound and improves wound healing area percentage
F
1, P
1value is relatively gained of same phase point for the moment between group, F
2, P
2relatively gained of phase point when value is each in group; With the comparison of Oleum Sesami group,
ap<0.05; With the comparison of mupirocin group,
bp<0.05; With phase point comparison when last in group,
cp<0.05.With the comparison of Oleum Sesami group, raw skin line of oils wound healing time obviously shortens (P<0.05); After scalding, in 5～22 days, raw skin line of oils wound tissue healing percentage ratio is apparently higher than Oleum Sesami group (P<0.05).
Embodiment 8 is raw, and skin oil promotes wound surface skin regeneration, accelerates scar repairing:
Animal
Japan's health large ear rabbit, 9, male and female dual-purpose, body weight 2.2～2.5Kg, by providing from Wuhan Biological Products Inst..The single cage of rabbit is raised, and free drinking water diet is raised room temperature and remained on 25 ± 1 ℃, and the light and shade cycle is 12 hours.
1. the preparation of rabbit III degree scalding model and grouping administration
Conform after one week until rabbit, lumbar injection 0.3% sodium phenobarbital (15ml/kg) is anaesthetized, and with shaving a mao machine (CP-7800, Shenzhen Coudé scholar) by its back depilation, area is 5cm × 5cm.Then with electromagnetic oven by boiled water in kettle and keep the state of constantly boiling, Hu Kou is linked to 50ml by a Heat insulated conduit and removes mouth at the bottom of end centrifuge tube, (area is 6cm to the centrifugal mouth of pipe
2) closely press and touch at tested rabbit back (every rabbit 2 place's wound surface, the left back of the body, each 1 place of the right back of the body) skin of unhairing place, each steam scalds to press and continues 30 seconds, sets up III degree scalding model (detect and confirm through the pathology of this experiment scald wound skin histology).
Scald Burn Rabbits employing table of random number method is divided into three groups and comprises Oleum Sesami group, raw skin line of oils and mupirocin group, 3 every group, 2 wound surface of every rabbit, and smear respectively Oleum Sesami, give birth to skin oil (the raw skin oil 3 being made by embodiment 3) and mupirocin ointment, monolayer sterile gauze flap coverage.Hinder in latter 4 days the every day of each coating 1 time in the time of 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time in the time of 8:00,13:00,18:00, totally 3 times, until wound healing.
2. rabbit III degree scald wound pathological study
Rabbit was scalded after 45 days, the processing of discontinuing medication.After air tap inserting method is put to death rabbit, get each group of rabbit edge of wound place skin sample (1cm × 3cm) and be fixed in 10% formalin.After 24 hours, with tap water rinsing 3 hours, then gradient ethanol dehydration, routine paraffin wax embedding, section (thickness be 4 μ m), carry out HE dyeing.
3. experimental result
Scald after 45 days, as shown in Figure 3, Oleum Sesami group and mupirocin are formed face width and dark to the HE coloration result of wound tissue, and epidermis thickens, and subcutaneously have no new saccus setigerus, and cicatrization is to Musclar layer; Compared with first two groups, raw skin line of oils scald wound skin texture is complete, the i.e. newborn fine and close hair follicle of the appendages of skin that subcutaneous visible growth is complete, and newborn arrangement of collagen fibers is neat, no significant difference compared with normal skin tissue.
The raw skins oil of embodiment 9 raises the expression of the albumen such as FGF-2, TGF-β 1, VEGF in rabbit wound surface skins:
Because the pharmacological Mechanism for the treatment of burn and scald is not clear, make its present situation that cannot apply clinically based at present a lot of compound Chinese medicinal preparation, the mechanism that the present invention scalds raw skin oil treatment III degree is studied.It is reported that empyrosis wound surface reparation is regulated and controled by many factors, wherein abundant nutrient substance and oxygen supply are the bases of wound repair, after burn and scald, wound surface Microembolization makes tissue ischemia anoxia, to cause that wound surface is early stage to be deepened and one of downright bad major reason, can the microvascular reconstruction of wound surface is related to repair tissue obtain sufficient blood flow and oxygen confession, contribute to wound surface quickly-healing (Comparison of oxidative stress biomarkerprofiles between acute and chronic wound environments.Wound Repair Regen.2004, 12:419-429).Vascular endothelial cell growth factor (VEGF) is single-minded mitosis stimulating factor and chemotactic factor of endotheliocyte, and scalable vascular endothelial cell proliferation, migration, increase microvascular permeability, accelerates the formation of new vessels; FGF2 (FGF-2) is the very strong protein factor of a kind of Promote cell's growth effect, can stimulate propagation and the migration of capillary endothelial cell, lure into blood vessel endothelium grow at the bottom of gum base in and form and the similar tube chamber spline structure of blood capillary, thereby progressively produce new vessels; Transforminggrowthfactor-β1 (TGF β 1) is the at present known somatomedin the closest and the most representative with cicatrization, and it can promote the biosynthesis of vegf protein in fibroblast and vascular endothelial cell.
Animal
Japan's health large ear rabbit, 9, male and female dual-purpose, body weight 2.2～2.5Kg, is provided by Wuhan Biological Products Inst..The single cage of rabbit is raised, and free drinking water diet is raised room temperature and remained on 25 ± 1 ℃, and the light and shade cycle is 12 hours.
1. the preparation of rabbit III degree scalding model and grouping administration
Conform after one week until rabbit, lumbar injection 0.3% sodium phenobarbital (15ml/kg) is anaesthetized, and with shaving a mao machine (CP-7800, Shenzhen Coudé scholar) by its back depilation, area is 5cm × 5cm.Then with electromagnetic oven by boiled water in kettle and keep the state of constantly boiling, Hu Kou is linked to 50ml by a Heat insulated conduit and removes mouth at the bottom of end centrifuge tube, (area is 6cm to the centrifugal mouth of pipe
2) closely press and touch at tested rabbit back (every rabbit 2 place's wound surface, the left back of the body, each 1 place of the right back of the body) skin of unhairing place, each steam scalds to press and continues 30 seconds, sets up III degree scalding model (detect and confirm through the pathology of this experiment scald wound skin histology).
Scald Burn Rabbits employing table of random number method is divided into three groups and comprises Oleum Sesami group, raw skin line of oils and mupirocin group, 3 every group, 2 wound surface of every rabbit, and smear respectively Oleum Sesami, give birth to skin oil (the raw skin oil 4 being made by embodiment 4) and mupirocin ointment, monolayer gauze flap coverage.After wound front 4 interior every days each coating 1 time in the time of 8:00,10:00,12:00,14:00,16:00,18:00, totally 6 times; Scald after 4 days the every day of each coating 1 time in the time of 8:00,13:00,18:00, totally 3 times, until wound healing.
2. rabbit III degree scald wound histogenic immunity Fluorescent Staining Observation
Rabbit was scalded after 45 days, the processing of discontinuing medication.After air tap inserting method is put to death rabbit, get each group of rabbit edge of wound place skin sample (1cm × 3cm) and be fixed in 10% formalin.After 24 hours, with tap water rinsing 3 hours, then gradient ethanol dehydration, routine paraffin wax embedding, section (thickness be 4 μ m), carry out the immunofluorescence dyeing of VEGF, TGF β 1, FGF2.Immunofluorescence dyeing adopts lowlenthal serum sealing 30 minutes, then removes confining liquid, adds respectively TGF β 1, FGF2, and tri-kinds of polyclonal antibodies of VEGF (three kinds of antibody is all with the dilution proportion of 1:100), juxtaposition is cut into slices and is spent the night in 4 ℃ of refrigerator moisture keepings.PBS washes and after three times, adds Alxa Fluor 488 goat-anti rabbit IGg(with the dilution proportion of 1: 1000), room temperature moisturizing lucifuge are hatched 1 hour; Finally, PBS washes three sections, mounting, and under electronic fluorescence microscope, (Nikon Eclipse Ti, Japan) takes pictures.Evaluate the ability of raw skin oil to wound surface skin regeneration and reparation by the expression of the above-mentioned three kinds of cell growth factor of NikonNIS-element AR 3.1 software analysis.
3. experimental result
The scald that adopts the method for immunofluorescence dyeing to detect is respectively organized the TGF-β of rabbit wound surface place 1 for 45 days afterwards, FGF2, and the expression of the albumen such as VEGF as shown in Figure 4, is compared with mupirocin group with Oleum Sesami group, and the visible FGF2 protein expression of raw skin line of oils wound surface skin is higher.It is less that Oleum Sesami group and mupirocin are formed in surface skin TGF-β 1 protein expression; In raw skin line of oils wound surface skin, visible TGF-β 1 protein expression is obvious.Vegf protein is expressed less in Oleum Sesami group and mupirocin are formed surface skin; Compared with first two groups, the visible vegf protein of raw skin line of oils wound surface skin is expressed obviously and is increased.
Claims (3)
1. the raw skin oil of external for the treatment of burn and scald, is characterized in that being prepared from by the raw material of following weight portion proportioning:
Radix Scutellariae 15 ~ 25 weight portions, Radix Et Rhizoma Rhei 15 ~ 25 weight portions, the Radix Astragali 15 ~ 25 weight portions, Rhizoma Coptidis 15 ~ 25 weight portions, Cortex Phellodendri 15 ~ 25 weight portions, Radix Angelicae Sinensis 10 ~ 15 weight portions, Radix Sanguisorbae 10 ~ 15 weight portions, Radix Arnebiae (Radix Lithospermi) 10 ~ 15 weight portions, Radix Rehmanniae 6 ~ 10 weight portions, the Radix Angelicae Dahuricae 6 ~ 10 weight portions, Radix Cynanchi Atrati 6 ~ 10 weight portions, the Pseudobulbus Bletillae (Rhizoma Bletillae) 6 ~ 10 weight portions, Olibanum 10 ~ 15 weight portions, Myrrha 10 ~ 15 weight portions, Borneolum Syntheticum 3 ~ 8 weight portions, Oleum Sesami 800-1000 weight portion;
The preparation method of the described raw skin oil of external, its step is as follows:
Oleum Sesami is inserted in container, then add Radix Scutellariae, Radix Et Rhizoma Rhei, the Radix Astragali, Rhizoma Coptidis, Cortex Phellodendri, Radix Angelicae Sinensis, Radix Sanguisorbae, Radix Arnebiae (Radix Lithospermi), Radix Rehmanniae, the Radix Angelicae Dahuricae, Radix Cynanchi Atrati, the each medicinal powder of the Pseudobulbus Bletillae (Rhizoma Bletillae), stir;
Soak after 3-10 day, agitating heating, maintenance oil temperature is 55-65 ℃, 1-2 hour turning off stove, after filter and remove residue, adds Olibanum and Myrrh, 55-65 ℃ is stirred 5-30 minute, from fire, filter and remove residue, in the time that oil temperature is down to 30-40 ℃, add Borneolum Syntheticum, fully stir evenly, be cooled to room temperature, bottled rear sealed storage.
2. the raw skin oil of external claimed in claim 1 is in the application of preparing in burn and scald medicine.
3. the application of the raw skin oil of external claimed in claim 1 in the medical apparatus and instruments for the preparation of burn and scald treatment.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1075877A (en) * | 1992-05-10 | 1993-09-08 | 株洲市财贸职工医院 | A kind of preparation method of burn ointment |
| CN1899441A (en) * | 2006-06-28 | 2007-01-24 | 广东皮宝制药有限公司 | Chinese medicine paste for burn and its preparing method |
| CN1899518A (en) * | 2006-07-13 | 2007-01-24 | 李先奇 | External use Chinese medicine for treating burn and scold and its preparing method |
| CN102416084A (en) * | 2011-11-25 | 2012-04-18 | 冯云萍 | Externally applied drug for treating bedsores, burns and scalds |
-
2012
- 2012-10-31 CN CN201210426168.XA patent/CN102895526B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1075877A (en) * | 1992-05-10 | 1993-09-08 | 株洲市财贸职工医院 | A kind of preparation method of burn ointment |
| CN1899441A (en) * | 2006-06-28 | 2007-01-24 | 广东皮宝制药有限公司 | Chinese medicine paste for burn and its preparing method |
| CN1899518A (en) * | 2006-07-13 | 2007-01-24 | 李先奇 | External use Chinese medicine for treating burn and scold and its preparing method |
| CN102416084A (en) * | 2011-11-25 | 2012-04-18 | 冯云萍 | Externally applied drug for treating bedsores, burns and scalds |
Non-Patent Citations (10)
| Title |
|---|
| > * |
| < * |
| .2004,第13卷(第4期),38-40. * |
| .2012,第55卷(第9期),889-890,910. * |
| 中医外治杂志> * |
| 人民军医> * |
| 李彦秋等.中药外用治疗烧烫伤进展.< * |
| 李彦秋等.中药外用治疗烧烫伤进展.<<人民军医>>.2012,第55卷(第9期),889-890,910. |
| 郭静等.外用中医药制剂治疗烧伤的进展.< * |
| 郭静等.外用中医药制剂治疗烧伤的进展.<<中医外治杂志>>.2004,第13卷(第4期),38-40. |
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Effective date of registration: 20160624 Address after: 518000 Guangdong city of Shenzhen province Futian District Shatou street Che Kung Temple Tairan six road cedar building B, 9A Patentee after: Shenzhen origin Biotechnology Co., Ltd. Address before: 430074 School of pharmacy, South-Central University For Nationalities, 708 National Road, Hongshan District, Hubei, Wuhan Patentee before: Tang Hebin |