CN115844992B

CN115844992B - External skin care oil for preventing and treating eczema and pruritus as well as preparation method and application thereof

Info

Publication number: CN115844992B
Application number: CN202211585608.6A
Authority: CN
Inventors: 唐和斌; 马巳媛; 谭港宁; 张朝; 张君月; 杨贵淳; 唐晟杰; 李玉桑
Original assignee: South Central University for Nationalities
Current assignee: South Central Minzu University
Priority date: 2022-12-09
Filing date: 2022-12-09
Publication date: 2023-11-17
Anticipated expiration: 2042-12-09
Also published as: CN115844992A

Abstract

The invention relates to the technical field of new indications of medicines, and in particular discloses external skin care oil for preventing and treating eczema and pruritus as well as a preparation method and application thereof. The external skin care oil is prepared by mixing the following raw materials in parts by weight in a colloid mill, flash extraction and other modes: 30-40 parts of astragalus membranaceus, 30-40 parts of angelica sinensis, 30-40 parts of gynura procumbens, 30-40 parts of garden burnet, 15-20 parts of frankincense, 15-20 parts of bletilla striata, 15-20 parts of angelica dahurica, 15-20 parts of cynanchum atratum, 10-20 parts of myrrh, 10-20 parts of scutellaria baicalensis, 10-20 parts of coptis chinensis, 10-20 parts of phellodendron amurense, 10-20 parts of rheum officinale, 3-5 parts of borneol and 1000-1500 parts of edible vegetable oil. Scientific experiments prove that the external skin oil preparation has better effects of tonifying qi and nourishing blood, activating blood and removing stasis, purging pathogenic fire and removing toxin, removing necrotic tissue and promoting granulation, dispelling wind and relieving itching, can promote rapid regression of acute and chronic eczema symptoms of rats induced by DNFB in a short time, and can also rapidly inhibit itching signs of mice induced by the combined use of histamine and 4-aminopyridine.

Description

External skin care oil for preventing and treating eczema and pruritus as well as preparation method and application thereof

Technical Field

The invention relates to the technical field of new indications of medicines, in particular to external skin care oil for preventing and treating eczema and pruritus, and a preparation method and application thereof.

Background

Eczema is a skin disease characterized by localized and polytype rash and exudation tendency or infiltration and hypertrophy of the superficial epidermis and dermis caused by various internal factors (such as chronic digestive system diseases, endocrine dyscrasia or mental stress, insomnia, overfatigue, emotional changes and the like) and external factors (such as environment, climate changes, sunlight, various animal fur, plants, cosmetics and the like), and is accompanied by severe itching and easy repeated attacks. The incidence of eczema is highest among all skin diseases. Meanwhile, skin injury can be caused by eczema, large-area skin injury and cumulus herpes are generated by acute eczema, and exudation tends to occur; the skin of the affected part of the chronic eczema is obviously thickened, mossiness appears, and the chronic eczema is easy to repeatedly attack, so that the daily work and life of a patient are very affected.

At present, eczema is classified as allergic diseases by Western medicine clinic, and the treatment principle is that pruritus is relieved as soon as possible, skin inflammation is controlled, skin barrier function is recovered, and accordingly life quality of patients is improved. The Western medicine adopts internal and external treatment methods, and usually suggests that patients take antihistamines such as chlorphenamine, cetirizine, loratadine, olopatadine hydrochloride, terfenadine and the like orally, and simultaneously combines external glucocorticoids (such as prednisone, compound dexamethasone acetate emulsifiable paste and the like) and calcium-modulated nerve phosphate inhibitors and calcium preparations (such as calcium lactate, calcium gluconate and the like) of non-hormonal therapeutic drugs, or is matched with local external washing such as calamine lotion (used in the case of no exudates at first time), 3% boric acid solution (used in the case of more exudation or erosion occurrence) and the like. Although the above-mentioned therapies and medicines are easy to use, their clinical actual therapeutic effects are not ideal, and long-term repeated use may cause adverse reactions such as skin atrophy, pigmentation, etc., and the medicines are easy to relapse after stopping, even cause endocrine and metabolic disorders, and are difficult to control well.

The traditional Chinese medicine considers that eczema belongs to the type of wet sores, and is caused by damp evil accumulating and heat transforming, and then the damp heat soaks the skin. Traditional Chinese medicine in the past of China mostly considers that the traditional Chinese medicine has weak intrinsic endowment and deficient healthy qi, and wind, damp and heat attack are carried out on the skin for a long time, so that the skin is malnourished to form the traditional Chinese medicine: (1) The ancient Chinese medicine believes that the pathogenesis of eczema mainly comprises congenital deficiency, spleen qi weakness, exogenous wind, dampness, heat and the like; hurting heart and spleen; the itch and weakness are that the heart fire is excessive and the white scraps are spleen qi deficiency; (2) Modern Chinese medicine families consider that the disease is caused by congenital deficiency, fetal toxicity and heat, and heat stagnation in skin; or spleen dysfunction caused by acquired unbalanced feeding and improper diet, and then infection of wind, heat, dampness and toxin, and accumulation of pathogenic toxin in skin; repeated attacks, prolonged lasting or emotional injury, spleen deficiency, qi and blood, biochemical fatigue, yin blood and primordial qi consumption, blood deficiency, wind dryness and skin malnutrition. Therefore, in the aspect of treatment, some medicines with functions of clearing heat and detoxicating and astringing are selected: cortex Phellodendri, radix Sophorae Flavescentis, herba Portulacae, radix Arnebiae, flos Chrysanthemi Indici, etc.; when exudation is less, powder is selected to be applied externally, such as indigo naturalis powder; the skin in chronic stage can be selected based on skin caring cream due to hypertrophic moss, and is mainly selected as lotion (such as Ganlin lotion) or self-formulated fumigation lotion, emulsion or ointment, so that the composition for clearing heat, promoting diuresis and relieving itching is mainly selected during treatment. However, it is often difficult to control symptoms in a timely, effective and rapid manner for acute episodes of eczema.

To date, no matter in Western medicine or traditional Chinese medicine, aiming at the characteristics of miscellaneous causes, more syndromes and easy repetition of eczema clinically, a specific medicine which is simple and convenient to use, has no toxic or side effect and quick and obvious in curative effect, in particular to an external medicine, is still lacking. Therefore, it is also a main object of the present invention to actively search for a drug capable of reducing inflammatory cell recruitment and promoting recovery of skin barrier function, and an effective drug suitable for various eczema treatments, and to find a breakthrough eczema treatment method, which is an important point of attention of many pharmaceutical students.

Disclosure of Invention

Aiming at the problems of the prior art that special medicines for preventing and treating eczema and pruritus are lacking:

the first aim of the invention is to provide the external skin care oil which can effectively prevent and treat eczema and pruritus.

The second aim of the invention is to provide a preparation method of the external skin care oil for preventing and treating eczema and pruritus.

The third purpose of the invention is to provide the medical application of the external skin care oil for preventing and treating eczema and pruritus.

The inventive concept of the present invention is as follows:

eczema is a skin disease characterized by localized and polytype rash and exudation tendency or infiltration and hypertrophy of the superficial epidermis and dermis caused by various internal factors (such as chronic digestive system diseases, endocrine dyscrasia or mental stress, insomnia, overfatigue, emotional changes and the like) and external factors (such as environment, climate changes, sunlight, various animal fur, plants, cosmetics and the like), and is accompanied by severe itching and easy repeated attacks. The incidence of eczema is highest among all skin diseases.

It can be classified into infantile eczema and adult eczema according to the age of the affected group. The course of eczema usually begins in childhood. It is counted that the incidence of eczema is high, about 30% in developed countries in children under 12 years of age. Although the incidence of eczema in adults is lower than that in children, the incidence of eczema is also close to 10%, and the prevalence of eczema in developing countries tends to increase. About 1 million eczema patients in China calculated as 10% incidence, which is undoubtedly a very large figure. Once the acute eczema in childhood is improperly treated or is not cured for a long time, the acute eczema is often aggravated in adult stage, and intractable pruritus, pain, infection and other more vexation symptoms are caused.

According to the course of the disease and the corresponding pathological characteristics (exudation or infiltration, hypertrophy), eczema is classified into three different types, namely acute, subacute and chronic. Generally, on the premise of timely and correct treatment, the acute eczema is better within 6 weeks. If it does not survive, it can be considered chronic eczema for children over 3 months, and for people over half a year.

From the pathological changes of the tissue structure of local skin, the epidermis of the affected part of the patient suffering from acute eczema is spongy edema-shaped, blisters are formed in the layers of the thorns and under the layers of the horns, and local lymphocyte infiltration and neutrophil infiltration can be seen; its dermal superficial small vessel dilates and perivascular mild lymphocyte-based inflammatory cell infiltrates. Once it has deteriorated to subacute, even chronic eczema, the epidermis may show thickening, hyperkeratosis, marked spongiform edema, marked inflammatory cell infiltration around small blood vessels in the superficial dermis, increased capillary number, even abnormal changes such as endothelial cell swelling and hyperplasia.

At present, the clinical prevention and treatment of eczema by western medicine takes the treatment principle of relieving itching, controlling skin inflammation and recovering skin barrier function as soon as possible, thereby improving the life quality of patients. The Western medicine adopts internal and external treatment methods, and usually suggests that patients take antihistamines such as chlorphenamine, cetirizine, loratadine, terfenadine and the like orally, and simultaneously combines calcium-regulating nerve phosphate inhibitor and calcium preparation of external glucocorticoid and non-hormone therapeutic drugs, or combines local external washing such as calamine lotion, 3% boric acid solution and the like. Although the above-mentioned therapeutic methods and medicines are easy to use, their clinical actual therapeutic effects are not ideal, even various adverse reactions are produced, and the disease condition is easy to repeatedly attack and difficult to control.

For eczema, ancient medical staff in China name "sores", "embroidery ball wind", "four-bend wind", "tinea milk" and the like according to the disease symptoms and different parts, and the descriptions are similar to western medicine acute eczema, periaural eczema, eczema scrotum, atopic dermatitis, infantile eczema and the like. Although eczema has complex etiology and more influencing factors, traditional Chinese medicine in the past mostly considers that the traditional Chinese medicine has weak intrinsic endowment and deficient vital qi, and wind dampness and heat attack the skin for a long time, and the skin is malnourished. The dialectical typing is also commonly used in terms of damp-heat syndrome, blood-heat syndrome, spleen deficiency and dampness accumulation, blood deficiency and wind dryness, and is respectively modified by the following symptoms of gentian liver-purging decoction for clearing damp-heat, blood-cooling and dampness-removing decoction for clearing heat and cooling blood, dampness-removing stomach-poria decoction for benefiting spleen deficiency and dampness accumulation, angelica drink for replenishing blood and dispelling wind, etc.

In the "Jingyue complete book", the open-ancestor Zhang Zhangyue mentions: although the internal treatment method has the advantages that the decoction is slow and the pain is difficult to treat, the external application of the decoction is necessary for treating the severe acute and acute abscess. The Qing dynasty famous doctor Xuling embryo also has clouds in the medical source theory and peri-drug theory written by Qing dynasty famous doctor: the surgical methods are the most serious external treatment, among which, surrounding herbs are indicated. According to the characteristics of the diseases such as superficial eczema and exposed focus, the external application of the traditional Chinese medicine can directly reach the focus, plays roles of penetrating the skin, dredging the channels and collaterals, promoting blood circulation to remove blood stasis, and achieves the effects of eliminating pathogenic factors, strengthening the body resistance and repairing skin barriers. The external treatment method of traditional Chinese medicine has the characteristics and advantages of traditional Chinese medicine, and really achieves better curative effect in the aspect of treating eczema.

Of course, it is known from the definition of eczema that skin itching is one of its important clinical manifestations, more severely affecting the quality of life of the patient, and the most important signs of timely effective treatment are urgently needed.

With the scientific understanding of the neuroanatomy and neurophysiology of itch in depth, the international forum for study of itch formulated and promulgated a standardized two-step clinical classification of itch: (1) based on the first step of clinical presentation. It comprises the following steps: inflammatory skin disease-derived itching, non-inflammatory skin disease-derived itching, and itching associated with skin lesions secondary to chronic scratching; (2) a second step based on the underlying disease. It comprises the following steps: dermatological, systemic, neurological, psychiatric, mixed and other uncertain etiologies. However, since the start is late, scientific research and theoretical cognition on pruritus are relatively late, so that the current theory is still in a to-be-perfected stage, and various phenomena related to pruritus cannot be fully explained. Despite the therapeutic guidelines for itch, there is a substantial complexity of the etiology of the itch causing the itch and a large number of influencing factors, such that there is still a clinical lack of effective personalized (often considering patient age, underlying disease, history of past use, severity of itch and impact on patient quality of life) therapeutic strategies.

For the treatment of pruritus, western medicine clinical application first emphasizes avoiding contact with allergen, disabling suspected drugs and effectively treating primary disease, while simultaneously treating with antihistamine H ₁ And H ₂ Receptor antagonists, glucocorticoids (for children population, there is no standard regimen of external glucocorticoids), immunomodulation, etc. are the main factors, but often the adverse reactions cannot be used for a long time, so that itching recurs quickly after treatment is interrupted.

According to the symptoms, secondary skin damage, attack seasons and the like, traditional Chinese medicine considers that skin itch is caused by wind at the beginning and enters blood for a long time, and the skin itch is divided into two major types of wind syndrome and blood system syndrome. Wherein, the wind syndrome can be further typed according to the signs of dryness and dampness: if the skin is dry and has lichen-like appearance and wind dryness, the prescription should be added with drugs for moistening dryness and dispelling wind such as fructus Tribuli, periostracum Cicadae, semen Sesami and pollen; for instance, the eczema is caused by skin damp rot, and the dosage of the medicine should be taken into consideration such as dampness-resolving and heat-clearing, for example, kuh-seng, phellodendron bark, broom cypress fruit, dittany bark and the like. The blood system syndrome is subdivided into blood heat and blood deficiency syndrome: the patients in Qing Zhuang year usually take blood heat as the main syndrome, and the therapy should follow the law of cooling blood and clearing heat; the elderly patients are mainly marked by blood deficiency, and the treatment is to nourish blood and moisten skin. If the blood stasis is not cured for a long time, the treatment of activating blood and dissolving stasis should be considered, and modified Taohong Siwu Tang should be used.

Based on decades of clinical experience and scientific research accumulation of traditional Chinese medicine pharmacology, the inventor of the technology considers that common pathogenesis of eczema and pruritus is formed by weak intrinsic endowment, deficiency of vital qi, invasion of wind dampness and heat evil and long-term accumulation of wind dampness and heat evil on skin, unsmooth qi and blood, loss of nutrition of skin and chronic ulcer. Therefore, the inventor of the technology provides unique prevention and treatment insight aiming at the pathogenesis formed by eczema and pruritus, namely, the product which has the effects of tonifying qi, activating blood, removing stasis, dredging collaterals, promoting wound healing, promoting granulation, dispelling wind, relieving itching, clearing heat, cooling blood, eliminating dampness and detoxifying is adopted, so that the effects of detoxification, corrosion removal and sore muscle growth are exerted, and the pain of eczema and pruritus is rapidly eliminated. The inventor of the technology has revealed in earlier work that the external skin oil for treating skin burns and scalds accords with the medicine principle and also has the estimated effect. Therefore, the inventor speculates that the external skin oil can also effectively treat eczema and pruritus, and the components are modified based on the original prescription, and the dosage of the reserved medicines is adjusted according to the treatment principle proposed by the applicant to form the prescription of the external skin oil for preventing and treating eczema and pruritus.

Therefore, the applicant carries out a great deal of scientific experiment and examination for preventing and treating eczema and pruritus by aiming at the skin oil, and researches the treatment effect of the prepared skin oil on the diseases through external local administration by respectively establishing animal models of acute eczema, chronic eczema and pruritus and also clinical acute eczema of cheeks and chronic eczema of perineum. Research results prove that the skin oil prepared by the application can promote acute and chronic eczema of rats induced by Dinitrofluorobenzene (DNFB), can rapidly inhibit itching signs of mice induced by combined medication of histamine and 4-aminopyridine, and can rapidly promote repair of skin tissue structures of wound surfaces of animals in various models; the prevention and treatment effect of the traditional Chinese medicine composition is obviously superior to that of a control medicine which is used in Western medicine and clinical practice of traditional Chinese medicine ((1) anti-inflammatory western medicine: external compound dexamethasone acetate emulsifiable paste), (2) Chinese patent medicine which has the functions of clearing heat and drying dampness, promoting blood circulation and dispelling wind, and relieving itching and diminishing inflammation: external compound phellodendron liquid, ice Huang Fule ointment and QINGQINGPENG ointment). The skin oil preparation of the application has obvious better effect on acute eczema of cheeks and chronic eczema of perineum than Western medicine or combined use of Chinese and Western medicine of famous and famous medicine (such as combined use of triamcinolone acetonide econazole cream, olopatadine hydrochloride tablet, shaggy grass tablet, ding-rou geranium ointment and ganlin lotion).

In order to achieve the first object, the present invention adopts the following technical measures:

an external skin care oil for preventing and treating eczema and pruritus is prepared from the following raw materials in parts by weight: 30-40 parts of astragalus membranaceus, 30-40 parts of angelica sinensis, 30-40 parts of gynura procumbens, 30-40 parts of garden burnet, 15-20 parts of frankincense, 15-20 parts of bletilla striata, 15-20 parts of angelica dahurica, 15-20 parts of cynanchum atratum, 10-20 parts of myrrh, 10-20 parts of scutellaria baicalensis, 10-20 parts of coptis chinensis, 10-20 parts of phellodendron amurense, 10-20 parts of rheum officinale, 3-5 parts of borneol and 1000-1500 parts of edible vegetable oil;

the edible vegetable oil is selected from at least one of sesame oil, peanut oil, coconut oil, soybean oil, linseed oil, castor oil and olive oil, preferably from at least one of sesame oil and coconut oil.

Preferably, the external skin care oil for preventing and treating eczema and pruritus is prepared from the following raw materials in parts by weight: 40 parts of astragalus, 40 parts of angelica, 40 parts of gynura procumbens, 40 parts of garden burnet, 20 parts of frankincense, 20 parts of bletilla striata, 20 parts of angelica dahurica, 20 parts of cynanchum atratum, 20 parts of myrrh, 10 parts of radix scutellariae, 10 parts of coptis chinensis, 10 parts of cortex phellodendri, 10 parts of rheum officinale, 5 parts of borneol and 1500 parts of edible vegetable oil.

Preferably, the external skin care oil for preventing and treating eczema and pruritus is prepared from the following raw materials in parts by weight: 30 parts of astragalus, 30 parts of angelica, 30 parts of gynura procumbens, 30 parts of garden burnet, 15 parts of frankincense, 15 parts of bletilla striata, 15 parts of angelica dahurica, 15 parts of cynanchum atratum, 10 parts of myrrh, 20 parts of scutellaria baicalensis, 20 parts of coptis chinensis, 20 parts of phellodendron amurense, 20 parts of rheum officinale, 3 parts of borneol and 1185 parts of edible vegetable oil.

In order to achieve the second object, the present invention adopts the following technical measures:

a preparation method of the external skin care oil for preventing and treating eczema and pruritus comprises the following steps: placing edible vegetable oil into a container, and adding dry powder of radix astragali, radix Angelicae sinensis, radix Gynostemmatis, radix Sangusorbae, olibanum, rhizoma Bletillae, radix Angelicae Dahuricae, radix Cynanchi Atrati, myrrha, scutellariae radix, radix et rhizoma Rhei, coptidis rhizoma and cortex Phellodendri; placing the container for containing the medicinal materials in a colloid mill, and circularly grinding for 2-5h at normal temperature; cutting at 20000rpm for 3-5 times (5 min each time) after stopping, sieving with 5000 mesh sieve under negative pressure, collecting filtered oil, adding Borneolum Syntheticum, and stirring to obtain topical skin care oil for preventing and treating eczema and pruritus.

In order to achieve the third object of the present invention, the present invention also adopts the following technical measures:

the invention also provides application of the external skin care oil in preparing a medicament for preventing and treating eczema and pruritus.

The eczema and pruritus are caused by sensitization and/or inflammatory drugs.

Preferably, the eczema is acute eczema of cheek or chronic eczema of perineum.

The formula principle of each traditional Chinese medicine raw material of the invention is as follows:

astragalus root, chinese angelica, gynura procumbens, sanguisorba, frankincense, myrrh and the like can tonify qi, activate blood, remove blood stasis, remove meridian obstruction, cool blood and heal sores and are used as monarch drugs; the bletilla striata, the cynanchum atratum, the angelica dahurica and the like can reduce swelling, expel pus, remove putrefaction and promote tissue regeneration, and dispel wind and relieve itching, and are used as ministers; baical skullcap root, rhizoma Coptidis, cortex Phellodendri, radix et rhizoma Rhei, etc. can clear heat and purge fire, remove dampness, detoxify, and are used as adjuvants; the borneol and the vegetable oil are blended with the medicines to achieve the effects of detoxification, granulation promotion, wind dispelling and itching relieving.

The model of eczema and pruritus is selected and evaluated as follows:

acute eczema: (1) DNCB-induced acute eczema model of rats; (2) Certain diagnosis of cheek acute eczema cases are carried out by the X-th hospital dermatology department of Wuhan City;

chronic eczema: (1) DNCB-induced model of chronic eczema in rats; (2) Senile chronic perineum eczema diagnosed by a doctor of the King of medical department of certain people in Yueyang city;

Pruritus model: a model of mouse itch induced by combination of histamine and 4-aminopyridine;

evaluation index: (1) General observations (including scores) of affected skin of acute and chronic eczema rats; (2) HE staining observation and pathological analysis (including scoring) of the affected tissues of rats with acute and chronic eczema; (3) Itching frequency and HE staining of affected tissues of the itching model mice were observed and analyzed for pathology (including scoring).

Compared with the prior art, the invention has the following advantages and effects: the research result shows that the skin-care oil prepared by the invention can effectively utilize the products of tonifying qi, activating blood, removing blood stasis, dredging collaterals, healing sore, promoting granulation, dispelling wind, relieving itching, clearing heat, cooling blood, drying dampness, removing toxin after being externally applied for preventive and therapeutic use, so as to exert the effects of detoxification, removing putrefaction and promoting wound healing, and rapidly eliminate the bitter of eczema and pruritus. The control effect of the composition is obviously superior to that of western medicine and the control medicines commonly used in clinical medicine ((1) anti-inflammatory, antibacterial and antiallergic western medicines: compound dexamethasone acetate cream; triamcinolone acetonide econazole cream; olopatadine hydrochloride; 2) Chinese patent medicines having the functions of clearing heat and drying dampness, promoting blood circulation and dispelling wind, relieving itching and diminishing inflammation, and the composition comprises external compound phellodendron liquid, ice Huang Fule ointment, qixingqingpeng ointment, dingrou geranium ointment and Ganzhen lotion).

The skin care oil has the following advantages for preventing and treating eczema and pruritus: (1) the cost of the medicine is low; (2) the external use is convenient and the effect is quick; (3) has good rehabilitation effect on acute eczema of cheek and chronic eczema of perineum.

Drawings

FIG. 1 is a graph showing the effect of a test drug on the skin of an affected part of acute eczema and the degree of damage thereof. A is the influence of skin oil (1) (SFO 1), skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXSS) and compound phellodendron bark liquid (HBXJ) on the appearance of the skin of the affected part of the experimental rat and the evaluation chart thereof; b is the effect of Bletilla striata extract (Bletilla striata), olibanum extract (Fankindense) and Bletilla Striata Polysaccharide (BSP) on the skin appearance of affected part of experimental rat and its evaluation chart. The figures identify P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scale in the figure is 1 cm.

FIG. 2 is a graph showing the effect of a test drug on the pathological structure of the skin of an affected part of acute eczema and the pathological evaluation thereof. A is the influence of skin oil (1) (SFO 1), skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXSS) and compound cortex Phellodendri liquid (HBXJ) on the pathological skin tissue of the affected part of the experimental rat (HE dyeing result) and the evaluation chart thereof; b is the influence of Bletilla striata extract (Bletilla striata), olibanum extract (Fankindense) and Bletilla Striata Polysaccharide (BSP) on the pathological tissue of the affected part of the experimental rat skin (HE staining result) and its score. The white line segments in the figure represent the thickened skin tissue epidermis layer of the rat; white arrows indicate inflammatory cell infiltration; white open arrows indicate subcutaneous fibrous tissue; the figures identify P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scale bars are 200 micrometers (objective:. Times.4) and 50 micrometers (objective:. Times.20).

FIG. 3 is a graph showing the effect of a test drug on the skin of a chronic eczema affected part and the degree of damage thereof. A is the influence of skin oil (1) (SFO 1), skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXSS) and Binghuangfule (BHFL) on the skin appearance of the affected part of the experimental rat and the evaluation chart thereof; b is the effect of Bletilla striata extract (Bletilla striata), olibanum extract (Fankindense) and Bletilla Striata Polysaccharide (BSP) on the skin appearance of affected part of experimental rat and its evaluation chart. The figures identify P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scale in the figure is 1 cm.

FIG. 4 is a graph showing the effect of a test drug on the pathological structure of the skin of the affected part of chronic eczema and the pathological evaluation thereof. A is the influence of skin oil (1) (SFO 1), skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXSS) and Binghuangfule (BHFL) on the pathological skin tissue of the affected part of the experimental rat (HE dyeing result) and a scoring graph thereof; b is the influence of Bletilla striata extract (Bletilla striata), olibanum extract (Fankindense) and Bletilla Striata Polysaccharide (BSP) on the pathological tissue of the affected part of the experimental rat skin (HE staining result) and its score. The white line segments in the figure represent the thickened skin tissue epidermis layer of the rat; white arrows indicate inflammatory cell infiltration; white open arrows indicate subcutaneous fibrous tissue; the figures identify P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scale bars are 200 micrometers (objective:. Times.4) and 50 micrometers (objective:. Times.20).

FIG. 5 is a graph showing the effect of test drug on the frequency of experimental mouse scrapings. A is the influence of skin oil 1 (SFO 1), skin oil 2 (SFO 2), compound dexamethasone acetate cream (DXSS) and Qingpeng ointment (QP) on the scrapings frequency of experimental mice; b is the effect of Bletilla striata extract (Bletilla striata), olibanum extract (Fankindense) and Bletilla Striata Polysaccharide (BSP) on the frequency of experimental mice pruritus. A "-1" of the horizontal axis identifies the test result before molding; * The symbols P <0.05, 0.01 and 0.001 are respectively the differences between the test drug group and the model group at the corresponding time points.

FIG. 6 is the effect of test drugs on pathological changes in skin structure at the site of scrapie in experimental mice. A is the influence of skin oil 1 (SFO 1), skin oil 2 (SFO 2), compound dexamethasone acetate cream (DAMS) and Qingpeng ointment (QP) on the pathological changes of skin structures of the experimental mouse pruritus area (HE staining chart and pathological scoring chart at the lower right); b is the effect of Bletilla striata extract (Bletilla striata), olibanum extract (Fankindense) and Bletilla Striata Polysaccharide (BSP) on pathological changes of skin structure of the experimental mouse pruritus area (HE staining chart and lower right pathological scoring chart). White line segments in the HE pathology plot indicate epidermal thickening in the HE staining plot; white filled arrows indicate inflammatory cell infiltration; white open arrows indicate subcutaneous fibrous tissue. * The P <0.01 and 0.001 are identified as the differences between the test drug group and the model group at the corresponding time points, respectively. The scale bars in the figure are 100 micrometers (objective:. Times.4) and 50 micrometers (objective:. Times.20).

Fig. 7 is a perineal eczema case of 81 years old male patient. The left graph shows the state after one month of treatment with hormone western medicines during hospitalization (family transfer: slightly improved affected part color, swelling and itching degree compared with the state before treatment); the right graph shows the hospitalization period, and the skin oil is applied topically for ten days (the color of the affected part is basically reduced, the swelling is basically reduced, the family members turn to say that the affected part is not scratched by the patient, and the symptoms of pruritus are understood to be reduced).

Fig. 8 is a right cheek eczema case of a 40 year old female patient. The left graph shows the state (the red swelling of cheek skin, accompanied by a large amount of blisters and exudation of yellow liquid, and the symptoms such as itching pain and difficulty are not basically resolved) after two days of treatment by the X-th hospital dermatology department (the important special department of Wuhan, the combination of Chinese and Western medicine and the "four-intensity" department of the national dermatology). The right image shows the state of applying the skin oil for 10 hours (i.e. the next morning is conscious that the right cheek is not painful, itchy or red, and the broken portion has crusted).

Detailed Description

The following applicant will make further details of the technical solution of the present application in connection with specific embodiments, in order to make the understanding and appreciation of the present application more clear to those skilled in the art.

Example 1: a preparation method of topical skin care oil for preventing and treating eczema and pruritus comprises the following steps.

Placing 1.62L sesame oil into a container, then adding 40g of dry powder of radix astragali, radix Angelicae sinensis, gynura procumbens and radix Sanguisorbae respectively, 20g of dry powder of radix Angelicae Dahuricae, radix Cynanchi Atrati, rhizoma Bletillae, olibanum and Myrrha respectively, 10g of dry powder of radix Scutellariae, radix et rhizoma Rhei, rhizoma Coptidis and cortex Phellodendri respectively, and stirring well; and (3) placing the container for containing the medicinal materials in a colloid mill, and circularly grinding for 5 hours at normal temperature. Cutting at 20000rpm for 3 times (5 min each time) by flash extraction device after stopping; sieving with 5000 mesh sieve under negative pressure, collecting filtered oil, adding Borneolum Syntheticum 5g into the filtered oil, stirring to obtain topical skin care oil (1), packaging in 100ml sterilizing bottle, and sealing for storage.

In addition, as a comparison:

preparation of bletilla striata extract: placing 1.62L sesame oil into a container, then adding 20g of dry powder of bletilla striata, and uniformly stirring; and (5) placing the mixture into a colloid mill and circularly grinding the mixture for 5 hours at normal temperature. After stopping, the mixture is sheared for 3 times (5 min each time) at a high speed by a flash extraction device of 20000 rpm; sieving with 5000 mesh sieve under negative pressure, collecting filtrate to obtain extract of rhizoma Bletillae for external use, packaging in 100ml sterilizing bottle, and sealing for storage.

Preparation of Boswellia carterii extract: placing 1.62L sesame oil into a container, then adding 20g of dry powder of frankincense, and uniformly stirring; and (5) placing the mixture into a colloid mill and circularly grinding the mixture for 5 hours at normal temperature. After stopping, the mixture is sheared for 3 times (5 min each time) at a high speed by a flash extraction device of 20000 rpm; sieving with 5000 mesh sieve under negative pressure, collecting filtered oil to obtain Olibanum extract, packaging in 100ml sterilizing bottle, and sealing for storage.

Example 2: a preparation method of topical skin care oil for preventing and treating eczema and pruritus comprises the following steps.

Placing 1.28L sesame oil into a container, then adding 30g of dry powder of radix astragali, radix Angelicae sinensis, gynura procumbens and radix Sanguisorbae respectively, 20g of dry powder of radix Scutellariae, radix et rhizoma Rhei, rhizoma Coptidis and cortex Phellodendri respectively, 15g of dry powder of radix Angelicae Dahuricae, radix Cynanchi Atrati, rhizoma Bletillae, olibanum and Myrrha respectively, and stirring uniformly; and (3) placing the container for containing the medicinal materials in a colloid mill, and circularly grinding for 2 hours at normal temperature. After stopping, the mixture is sheared for 5 times (5 min each time) at a high speed by a flash extraction device of 20000 rpm; sieving with 5000 mesh sieve under negative pressure, collecting filtered oil, adding Borneolum Syntheticum 3g into the filtered oil, stirring to obtain topical skin care oil (2), packaging in 100ml sterilizing bottle, and sealing for storage.

Example 3: a preparation method of external bletilla striata polysaccharide for preventing and treating eczema and pruritus comprises the following steps.

Preparing a bletilla striata polysaccharide solution: (1) dissolving dry powder of rhizoma Bletillae polysaccharide (obtained from Vickers biotechnology Co., ltd. With purity of 90% or more) in ultrapure water, adding Tween 80 and glycerol into the obtained solution to obtain water phase containing rhizoma Bletillae polysaccharide (wherein the final concentration of Tween 80 is 1.25% V/V; and the final concentration of glycerol is 2.81% V/V); (2) the oil phase (final concentration of egg yolk lecithin is 6%W/V, g/mL) is prepared by dissolving egg yolk lecithin in sesame seed oil. (3) Under the condition of 60 ℃ constant temperature water bath, the volume ratio of the oil phase to the water phase is 1:4, the oil phase was dropped into the water phase and stirred at 1500r/min for 10min. (4) Shearing at 20000rpm for 2min, homogenizing at 1000bar under high pressure for 3 times to obtain rhizoma Bletillae polysaccharide solution, wherein the content of rhizoma Bletillae polysaccharide in the obtained rhizoma Bletillae polysaccharide solution is equal to 1mg/ml. Example 4 Effect of the skin oils (1) and (2) prepared in examples 1 and 2 on DNCB-induced acute eczema model of rats

The test animals were male, 36 SD rats of 6 week old SPF grade (supplied by Liaoning long Biotechnology Co., ltd.). The free drinking water diet is adopted, the temperature is kept at 25+/-1 ℃ during the feeding period, and the bright-dark period is 12 hours.

1) Grouping modeling and drug delivery

36 rats were randomly divided into 9 groups: blank group, model group, skin oil (1), skin oil (2), rhizoma Bletillae extract, olibanum extract, rhizoma Bletillae polysaccharide group, compound dexamethasone acetate cream group, and compound cortex Phellodendri liquid group.

After the rats are adaptively raised for 7 days, the hairs of the rats at the back experimental position are roughly cut off by a shaver close to the skin, the area of the exposed skin position is about 3cm multiplied by 3cm, the rats are dehaired again by using dehairing paste, and the rats are thoroughly washed by warm water immediately after dehairing, so that the skin at the experimental position is exposed for standby. After 1 day of dehairing, except that the blank group does not do any damage treatment, all rats in the other groups are subjected to ether inhalation anesthesia and placed on a fixed frame, and then 100 mu L of 7% DNCB acetone solution is used for external coating of naked back skin sensitization of the rats; after dehairing for 2 days, the application is strengthened for 1 time by the same method, and the sensitization is performed again; after depilating for 7 days, the exposed skin on the back of the rat was challenged with 20 μl of 1% dncb acetone solution by a pipette. The molding was successful with the appearance of obvious exudation, pimple, erythema, etc. in the back area of the rat.

On the day of administration, the wound sites of the model group rats were smeared with 0.25ml of physiological saline except for the blank group; applying 0.25ml of skin oil (1), skin oil (2), rhizoma Bletillae extract, olibanum extract, and rhizoma Bletillae polysaccharide (prepared in example 3) to wound parts of rats; compound dexamethasone acetate cream (Huarun Sanjiu medical Co., ltd.) 0.25ml is smeared on the wound part of each group of rats; compound cortex Phellodendri liquid composition 0.25ml of compound cortex Phellodendri liquid (Shandong Han prescription pharmaceutical Co., ltd.) is applied to the wound parts of rats of each group. The administration was 1 time per day, and 3 times in succession.

2) Skin general observations of rats tested before and after acute eczema modeling

Selecting 4 time periods of successful modeling, 1 day after administration, 2 days after administration, 3 days after administration and the like, and recording the back wound surface of the rat by a Sony single-reverse microspur camera to obtain a skin change map of the rat with the acute eczema of each period. The severity of back skin lesions (table 1) was scored against the skin sensitization test scoring criteria table for each group of rats, and during the evaluation, at least two persons (three persons in this example) were simultaneously and independently evaluated to avoid subjective factors, and the results were averaged.

Table 1. Skin sensitization assay scoring criteria table.

3) HE staining and pathological analysis of wounded tissue of test rats

After 3 days of administration, all the test animals were sacrificed by anesthetic method, the skin tissue of the wound site was removed, wrapped with gauze, and fixed in formalin solution for 24 hours. The skin tissue was then sequentially subjected to running water rinsing, dehydration, paraffin embedding, followed by tissue section, HE conventional staining, and observation under an optical microscope and photographing.

Pathological scoring of rat skin lesion tissue: rat skin tissues were pathologically scored under an optical microscope according to the following inflammatory scoring table (table 2) and expressed as mean±sem.

Table 2. Pathology scores of rat skin lesion tissue.

4) Experimental results

(1) General observation of skin of DNCB-induced acute eczema rats

As shown in fig. 1, during the period from the successful day of DNCB-induced acute eczema molding to 3 days after administration, the skin of the rats in the blank group was observed to be light red, fine, smooth and tender, soft in texture, and clear and visible in skin texture.

a) Severe erythema and severe edema, accompanied by exudation and eschar formation, occurred on the wound surface on the day after successful model creation in rats of model group: after 1 day of administration, the area of the eschar of the wound skin of the model group rat becomes smaller, and the scale with large area still slightly reduces the degree of erythema and edema; after 2 days of administration, the skin wound of the model group rat is further reduced, eschar still exists, the color of the crust is darkened, but the red spots and the edema are reduced to a degree which is 1 day later; after 3 days of administration, the wound surface still had a small portion of crusts, with more pronounced erythema and edema.

b) Compared with the model group, the skin erythema, edema and eschar of the rats in other groups are improved, particularly in the group of skin oil (1), after 1 day of administration, the skin eschar area of the rats is rapidly reduced, the color of the eschar is lighter, and the area of the erythema and edema is obviously reduced; after 2 days of administration, the skin of the group (1) rats has no edema and erythema around the scab surface of the skin; after 3 days of administration, skin scab and scale of the rats in group (1) of skin oil drop off.

c) Compared with the model group, the skin oil (2) group also has good treatment effect: after 1 day of administration, the skin crusta area of the rat is rapidly reduced, the crusta is lighter in color, and the erythema and edema degree are slightly reduced; after 2 days of administration, the skin scab surface of the skin of the rats in the group (2) of skin-growing oil becomes smaller; after 3 days of administration, skin scab skin and scales of rats in group (2) are mostly removed, only a small part is remained, and small area erythema is present.

d) Compared with the treatment effect of the skin oil (1), the compound dexamethasone acetate emulsifiable paste group and the compound phellodendron liquid group which simulate clinical medication are effective, but the effect of the skin oil (1) is still not different:

the wound surface skin of the rats in the compound dexamethasone acetate emulsifiable paste still has large-area eschar after 1 day of administration, and the color of the eschar skin is deeper than that of the skin oil (1); eschar drops off a small portion after 2 days of administration, accompanied by erythema edema; the crust remained incompletely detached after 3 days of administration, and was partially red and swollen.

The skin of the compound phellodendron bark liquid group rat still has large-area eschar after 1 day of administration; a small portion of eschar was left after 2 days of administration, with erythema edema; a small portion of the crust remained after 3 days of administration with red swelling.

As shown in fig. 1B, the bletilla striata extract, the olibanum extract and the bletilla striata polysaccharide, which are components of the skin-care oil and active monomers thereof, also show a certain therapeutic effect.

e) The skin of the rats with the bletilla striata extract group is scabbed and the color of the scab skin is deepened after 1 day of administration; the scab skin part falls off after 2 days of administration, and edema and erythema still exist around the scab surface; a small portion of crusts and erythema remained 3 days after administration.

f) And the large-area crust is still remained after the frankincense extract group rats are dosed for 1 day; a small portion of eschar was left after 2 days of administration, with edema; crusts remained and edema occurred 3 days after administration.

g) Compared with the recovery effect of the bletilla striata extract group and the olibanum extract group, the bletilla striata polysaccharide group prepared in the embodiment 3 has better performance: the crust color became dark and the eschar area became small after day 1 of administration; most of eschar and scale fall off after the 2 nd day of administration, and erythema and edema remain; after 3 days of administration, erythema and edema disappeared, leaving small areas of scale and crust without shedding.

From the time period from successful experimental modeling to 3 days after administration, the severity of back skin lesions was scored according to the skin sensitization experimental scoring criteria table (full score 14; score table 1), and the rats in the blank group were scored as 0 score, as can be seen from the acute eczema rat appearance scoring graph (fig. 1):

(1) The overall grading trend of the rats in the model group is slower from the successful modeling day to the 3 days after administration;

(2) The differences in scoring of the test drug groups are greater than the differences in scoring of the model groups:

after 1 day of administration, the skin appearance scores of rats in the skin oil (1) group (10.0.+ -. 0.4; P < 0.05) and the skin oil (2) group (11.0.+ -. 0.4) were both smaller than those in the model group (12.5.+ -. 0.3; P < 0.001), indicating that both skin oils (1) and (2) had repairability; and compared with the model group, the compound dexamethasone acetate emulsifiable paste group (11.3+/-0.5) and the compound phellodendron bark liquid group (12.3+/-0.3) have less obvious difference.

The appearance scores of the skin oil (1) group (3.2+ -0.4; P < 0.001) and the skin oil (2) group (4.3+ -0.3; P < 0.001) are significantly lower than those of the model group (10.0+ -0.4), the compound dexamethasone acetate cream group (6.0+ -0.4; P < 0.01) and the compound phellodendron liquid group (8.0+ -0.7) after 2 days of administration;

Skin of rats in the skin oil (1) group had tended to be thoroughly repaired (1.5.+ -. 0.3; P < 0.001) 3 days after dosing compared to the model group (8.5.+ -. 0.3); skin wound of rats in the group of skin oil (2) has little red swelling, but is also near recovery (2.0+/-0.4; P < 0.001); the skin of the rats still has crusts and red swelling in the compound dexamethasone acetate emulsifiable paste group (4.0+/-0.4; P < 0.01) and the compound phellodendron bark liquid group (3.5+/-0.5; P < 0.01).

In contrast, the data show that the skin-care oil (1) and the skin-care oil (2) have better repairing capability and treatment effect on skin than the compound dexamethasone acetate cream and the compound phellodendron bark liquid.

In addition, the applicant also studied the therapeutic effect of the components of the skin-care oil, bletilla striata extract group, olibanum extract group and bletilla striata polysaccharide group of active monomers (prepared in example 3) (fig. 1B), and found that the components of the skin-care oil are inferior to the skin-care oil (1) and the skin-care oil (2), but show a certain restoration trend compared with the model group.

The skin appearance scores of rats were smaller for the bletilla striata extract group (10.7.+ -. 0.2; P < 0.05), the olibanum extract group (11.0.+ -. 0.5), and the bletilla striata polysaccharide group (10.2.+ -. 0.3; P < 0.01) than for the model group (12.5.+ -. 0.3) 1 day after dosing;

the skin appearance scores of rats were closer in the bletilla striata extract group (6.0±0.4; p < 0.01), the olibanum extract group (7.0±0.4; p < 0.05), and the bletilla striata polysaccharide group (5.7±0.2; p < 0.01) than in the model group (10.0±0.4) after 2 days of dosing;

The comparison shows that the scores of the bletilla striata extract group (3.3+/-0.5; P < 0.01) and the bletilla striata polysaccharide group (2.8+/-0.4; P < 0.001) are closer than the scores of the rats in the model group (8.5+/-0.3) after 3 days of administration, and the effect of the olibanum extract group (4.3+/-0.5; P < 0.01) is slightly weaker than the effects of the bletilla striata extract group and the bletilla striata polysaccharide group.

(2) HE staining observation and pathological analysis of wound tissue of DNCB-induced acute eczema rat

After 3 days of administration (day 10 of the experiment), all rats tested were sacrificed by anesthesia, skin tissues of the wound were obtained, and HE staining was performed to obtain pathological analysis results as shown in fig. 2.

The cells of the epidermis and dermis layers of the rats in the blank group can be seen to be normally arranged, without edema, and a large number of hair follicles and other accessory organs can be seen without abnormal pathological morphological changes.

a) Compared with the blank control group, the skin tissue of the rat in the model group is presented: (1) the epidermis layer structure is incomplete, the proportion of the epidermis layer and the dermis layer is disordered, the cuticle layer and the particle layer are thickened, the acantha layer is hypertrophic, and the epidermis bulge is widened and prolonged; (2) the dermis layer is seen with massive sweat gland hyperplasia and inflammatory cell infiltration; (3) its pathology score of skin tissue (2.7.+ -. 0.5; P < 0.001) is significantly less than that of the blank (10.0.+ -. 0.0).

b) Compared with the model group, skin tissue of rats with acute eczema of skin oil (1) group appears: (1) the epidermis layer has complete structure, the cuticle is thinner than the model group, the epidermis process is not widened and prolonged, and the epidermis is slightly keratinized; (2) the dermis layer can grow hair through skin appendages such as hair follicles; (3) its skin histopathological score (7.8.+ -. 0.4; P < 0.001) is significantly greater than in the model group.

c) Compared with the model group, skin tissue of rats with acute eczema of skin oil (2) group appears: (1) the epidermis layer has complete structure, and the skin cuticle is obviously thinner than the model group; (2) the dermis layer can grow hair through a large number of skin appendages such as hair follicles; (3) its skin histopathological score (7.5.+ -. 0.6; P < 0.001) is significantly greater than in the model group.

d) However, compared with the model group, the curative effect of the compound dexamethasone acetate emulsifiable paste group is not obvious: (1) the epidermis layer structure is disordered and rough, the epidermis is hyperkeratosis, the thickening of the cuticle and the particle layer is not obviously improved compared with a model group, and the epidermis is widened and prolonged; (2) the dermis layer can grow hair through skin appendages such as hair follicles and the like, and is accompanied by massive inflammatory cell infiltration and erythrocyte overflow; (3) the skin tissue pathology score (4.7+ -0.5) was slightly higher than the model group.

e) Meanwhile, the treatment effect of the comparison model group and the skin oil (1) group and the skin oil (2) group and the compound phellodendron liquid group is not ideal: (1) although the epidermis layer has complete structure, the thickening of the stratum corneum and the thickening of the acantha layer are not obviously improved, and the epidermis bulge is prolonged; (2) the dermis layer can grow hair by skin appendages such as hair follicles and the like and is accompanied by inflammatory cell infiltration and erythrocyte overflow; (3) the pathological scores (6.0+ -0.4; P < 0.01) of the skin tissues were higher than those of the model group.

The bletilla striata, olibanum and bletilla striata polysaccharide, which are components of the skin-care oil and active monomers thereof, also exhibited a certain effect compared to the model group (fig. 2B).

f) Bletilla striata extract group rat skin tissue: (1) the whole structure of the skin is complete, the epidermis is obviously keratinized, and the stratum corneum and the granular layer are thickened; (2) the dermis layer can grow hair by skin appendages such as hair follicles and the like and is accompanied by moderate inflammatory cell infiltration, and congestion and edema among tissues; (3) the pathological scores (6.0+ -0.4; P < 0.001) of the skin tissues were higher than those of the model group.

g) Olibanum extract group rat skin tissue conditions were poor: (1) the whole structure of the skin is complete, the epidermis layer is obviously keratinized, the stratum corneum and the granular layer are thickened, the stratum spinosum is hypertrophic, and the epidermis is widened and prolonged; (2) the dermis layer is seen with massive sweat gland hyperplasia with moderate inflammatory cell infiltration and erythrocyte extravasation; (3) the skin tissue pathology scores (5.2+ -0.5; P < 0.01) were slightly higher than in the model group.

h) The effect of the skin tissue of the bletilla striata polysaccharide group is obvious in three groups: (1) the whole structure of the skin is complete, the stratum corneum of the skin is thinner than that of the model group, and the epidermis process is slightly widened and prolonged; (2) the dermis layer can grow hair by a large number of skin appendages such as hair follicles, and obvious inflammatory cell infiltration and erythrocyte overflow are not seen; (3) its skin histopathological score (6.5.+ -. 0.6; P < 0.001) is significantly greater than in the model group.

Example 5 Effect of the skin oils (1) and (2) prepared in examples 1 and 2 on DNCB-induced model of chronic eczema in rats

1) Grouping modeling and drug delivery

36 rats were randomly divided into 9 groups: blank group, model group, skin oil (1), skin oil (2), rhizoma Bletillae extract, olibanum extract, rhizoma Bletillae polysaccharide group, compound dexamethasone acetate cream group, and ice Huang Fule ointment group.

After the rats are adaptively raised for 7 days, the hairs of the rats at the back experimental position are roughly cut off by a shaver close to the skin, the area of the exposed skin position is about 3cm multiplied by 3cm, the rats are dehaired again by using dehairing paste, and the rats are thoroughly washed by warm water immediately after dehairing, so that the skin at the experimental position is exposed for standby. On day 1 after dehairing, except that the blank group does not do any damage treatment, all rats in the other groups are subjected to ether inhalation anesthesia and placed on a fixing frame, and then 100 mu L of 7% DNCB acetone solution is used for external coating of naked back skin sensitization of the rats; after 1 week, the back sheared area of the rat was again dehaired, and the next day was coated with 50 μl of 5% dncb acetone solution for the 2 nd sensitization. Sensitization was continued thereafter, 1 time every 5 days, 3 times in succession. The skin appearance of the rat back area, such as skin thickening, rough surface, pimple, red swelling, etc., is changed into modeling success.

On the 3 rd day after the successful modeling experiment, the wound part of the rats in the model group is smeared with 0.25ml of physiological saline except the blank group; applying skin oil (1), skin oil (2), rhizoma Bletillae extract, olibanum extract, and rhizoma Bletillae polysaccharide to the wound of rats, wherein each group contains 0.25ml of rhizoma Bletillae polysaccharide (1 mg/ml; rhizoma Bletillae polysaccharide solution prepared in example 3); the compound dexamethasone acetate emulsifiable paste group is used for smearing 0.25ml of compound dexamethasone acetate emulsifiable paste on the wound part of the rat; ice Huang Fule ointment group the wound site of the rat was coated with 0.25ml of ice Huang Fule ointment (tibetan sea Rong Tangguo pharmaceutical company limited). The administration was continued 3 times per day 1 time.

2) Skin general observations of rats tested before and after modeling of chronic eczema

Selecting the day of successful modeling and 3 time periods of 1 day after administration, 3 days after administration, and the like, recording the back wound surface of the rat by a Sony single-reverse microspur camera, and obtaining the skin change map of the rat with chronic eczema in each time period. The severity of skin lesions on the backs of the rats in each group was scored against the skin sensitization test scoring criteria table (table 1), and in the evaluation process, two individuals were simultaneously and independently evaluated to avoid subjective factors, and the results were averaged.

3) HE staining and pathological analysis of wounded tissue of test rats

After 3 days of administration, all the test animals were sacrificed by photographing and anesthesia, the skin tissue of the wound was removed, wrapped with gauze, and fixed in formalin solution for 24 hours. The skin tissue was then sequentially subjected to running water rinsing, dehydration, paraffin embedding, followed by tissue section, HE conventional staining, and observation under an optical microscope and photographing.

Pathological scoring of rat skin lesion tissue: rat skin tissues were pathologically scored under an optical microscope according to an inflammatory scoring table (table 2) and expressed as mean±sem.

4) Experimental results

(1) General observations of DNCB-induced chronic eczema rat skin

a) As shown in fig. 3, DNCB induced chronic eczema in rats, and the skin of the rats in the blank group was seen as pale red, fine and smooth, soft in texture, and clearly visible in the skin texture, until the period of 3 days after administration was successful.

b) After successful modeling, the wound surface of the model group rat has severe erythema and severe edema, and is accompanied by severe stripping and scaling: after 1 day of administration, the skin scale and the stripping area of the wound surface of the model group rat become smaller, and the erythema and edema degree are slightly reduced; after 3 days of administration, the skin wound surface of the model group rat is further reduced, scales and denudation still exist, and erythema and edema still exist.

c) Compared with the model group, the skin erythema, edema, scale and peeling of the rats in other groups are improved, particularly in the group (1) of raw skin oil, the skin scale and peeling area of the rats are rapidly reduced after 1 day of administration, the skin erythema and edema area is obviously reduced due to lighter crust color; after 3 days of administration, the skin of the rats in the group (1) has no edema and erythema, scales fall off, wound surfaces are hardly visible, and hair grows.

d) Meanwhile, compared with the model group, the skin-care oil (2) group also has good treatment effect: after 1 day of administration, the skin of the rat rapidly forms scab and drops the scab, the color of the scab skin is lighter, and the areas of erythema and edema are obviously reduced; the skin scab and scale of the rat in the group of skin oil (2) are completely removed after 3 days of administration, and only small area of erythema is generated.

e) Compared with the treatment effects of the skin oil (1) and the skin oil (2), the compound dexamethasone acetate cream group and the ice Huang Fule ointment group simulating clinical medication are more effective than the model group, but the effect of the compound dexamethasone acetate cream group and the ice Huang Fule ointment group separating from the skin oil group is still not slightly different:

the wound surface skin of the rats in the compound dexamethasone acetate emulsifiable paste still has large scale and the crust color is deeper than that of the skin oil (1) and (2) after 1 day of administration; a small portion of the crust remained undeployed after 3 days of administration with concomitant erythema edema.

Whereas the skin of the icy yellow skin ointment group rats remained mostly scales and the crusts were darker in color after 1 day of administration; the crust was not completely detached after 3 days of administration, with erythema edema.

In the research of the application, the bletilla striata extract, the frankincense extract and the bletilla striata polysaccharide which are components of the skin-care oil and active monomers thereof also show a certain effect.

f) The skin of the rats with the bletilla striata extract group forms scabs and the scab skin becomes dark after the 1 st day of administration, and erythema and edema are arranged around the scab surface; the scab part falls off after 3 days of administration, leaving a small part of scab, and there are erythema and edema around the scab.

g) The large scale, erythema and edema remained after 1 day of administration of the boswellia extract group rats; crusts remained and edema occurred 3 days after administration.

h) Compared with the recovery effect of the bletilla striata extract group and the olibanum extract group, the bletilla striata polysaccharide (1 mg/ml; the bletilla striata polysaccharide solution prepared in example 3) group performed better: the scab area becomes smaller after 1 day of administration; the scales largely fall off after 3 days of administration, leaving a small portion of crust, accompanied by a small area of erythema.

The severity of back skin lesions was scored (12 points full) for each group of rats and the blank group was scored as 0 points from the successful experimental modeling to 3 days post-dosing according to the skin sensitization experimental scoring criteria table.

a) According to the appearance scoring graph (figure 3) of the chronic eczema rats, the overall scoring trend of the rats in the model group from the day of successful modeling to 3 days after administration is slower;

b) Whereas the skin oil group score varies significantly:

1 day after administration, compared with a model group (10.0+/-0.4; P < 0.001), the skin appearance scores (5.0+/-0.4; P < 0.01) of the rats in the skin oil (1) group are smaller and are also smaller than the skin appearance scores (6.0+/-0.4; P < 0.01) of the rats in the skin oil (2) group, which indicates that the repair capability of the skin oil (1) and the skin oil (2) is good; the skin appearance scores of rats in the compound dexamethasone acetate cream group (8.8+/-0.5) and the ice Huang Fule ointment group (6.8+/-0.5; P < 0.05) have smaller change compared with the model group.

Skin of rats in the skin oil (1) group had tended to be thoroughly repaired (2.0.+ -. 0.4; P < 0.001) 3 days after dosing compared to the model group (6.7.+ -. 0.6); skin wound of rats in group (2) has little red swelling, but is also near recovery (2.7+ -0.2, P < 0.05).

c) The skin of rats with the compound dexamethasone acetate cream group (4.0+/-0.4) and the ice Huang Fule ointment group (3.5+/-0.6) still has crusts and red swelling. The comprehensive data show that the skin oil, namely the skin oil (1) or the skin oil (2), has better skin repair capability and treatment effect than the compound dexamethasone acetate cream and the ice Huang Fule ointment.

d) Compared with the model group, the bletilla striata extract group, the frankincense extract group and the bletilla striata polysaccharide group also show a certain restoration trend, but the treatment effect is inferior to that of the skin care oil group:

the skin appearance scores of rats were smaller in the bletilla striata extract group (6.8.+ -. 0.3; P < 0.001), the olibanum extract group (7.3.+ -. 0.7; P < 0.01), and the bletilla striata polysaccharide group (6.0.+ -. 0.4; P < 0.01) than in the model group (10.3.+ -. 0.3) after 1 day of dosing;

model group rats appearance score 7.3±0.4 3 days after dosing; by comparison among groups, the score of the bletilla striata polysaccharide group is the lowest (3.3+/-0.3; P < 0.01), and the effect of the frankincense extract group (4.5+/-0.7) is slightly weaker than that of the bletilla striata extract group (3.8+/-0.3; P < 0.01) and the bletilla striata polysaccharide group.

2) HE staining observation and pathological analysis of wound tissue of DNCB-induced chronic eczema rat

After 3 days of administration, all rats were sacrificed by anesthesia, skin tissues at the wound were collected and HE stained to obtain pathological analysis results as shown in fig. 4. The cells of the epidermis and dermis layers of the rats in the blank group can be seen to be normally arranged, without edema, and a large number of hair follicles and other accessory organs can be seen without any abnormal pathological morphological changes.

a) Compared with the blank control group, the skin tissue of the rat in the model group is presented: (1) the structure of the epidermis layer is fuzzy, the proportion of the epidermis layer and the dermis layer is unbalanced, obvious keratinization, thickening of the cuticle layer and the particle layer, thickening of the acantha layer and prolongation of the sudden increase of the epidermis; (2) inflammatory cell infiltration of dermis; (3) its pathology score of skin tissue (1.5.+ -. 0.3; P < 0.001) is significantly less than that of the blank (10.0.+ -. 0.0).

b) Compared with the model group, the skin oil (1) group (8.3+/-0.4; p < 0.001) chronic eczema rat skin tissue exhibited near normal skin tissue: (1) the epidermis layer structure is clear and complete, the epidermis layer of the skin is obviously thinner than the model group, and the skin is slightly keratinized; (2) the dermis layer can see the growth of appendages such as hair follicles and the like with a small amount of inflammatory cell infiltration; (3) its skin tissue pathology scores significantly more than the model group.

c) Compared with the model group, the skin oil (2) group (7.3+/-0.4; p < 0.001) chronic eczema rat skin tissue exhibited near normal skin tissue: (1) the epidermis layer structure is clear and complete, the epidermis layer of the skin is thinner than the model group, and the skin is slightly keratinized; (2) the dermis layer can grow with the appendages such as hair follicles; (3) its skin tissue pathology scores significantly more than the model group.

d) However, compared to the model group, the compound dexamethasone acetate cream group (3.8±0.5; p < 0.05) has insignificant effects: (1) the epidermis layer structure is fuzzy, the hyperkeratosis is carried out, the thickening of the cuticle and the particle layer is not obviously improved compared with a model group, and the thickness of the stratum spinosum is hypertrophic; (2) the dermis layer thickens, and fibrous hyperproliferation is seen with moderate inflammatory cell infiltration; (3) the skin tissue pathology score was slightly higher than in the model group.

e) Meanwhile, compared with the model group and the skin oil (1) and (2) groups, the ice Huang Fule ointment group (5.2+/-0.4; p < 0.01) is also less than ideal: (1) compared with a model group, the thickening of the epidermis layer is not obviously improved, the hyperkeratosis is excessive, the cell gap is thickened, the thickening of the acantha layer is not obviously reduced, and the epidermis bulge is prolonged; (2) the dermis is thickened, and fibrous hyperproliferation is seen with highly inflammatory cell infiltration and erythrocyte extravasation; (3) the pathology score of the skin tissue is higher than that of the model group.

Compared with the model group, the bletilla striata extract group, the frankincense extract group and the bletilla striata polysaccharide group also show a certain repairing curative effect:

f) Bletilla striata extract group rat skin tissue: (1) the epidermis layer is not clear, the epidermis layer is thickened, and the cell gap is thickened; (2) inflammatory cell infiltration exists in the dermis layer, and interstitial congestion and edema are caused; (3) the pathology scores (5.0+ -0.4; P < 0.001) of the skin tissues were higher than those of the model group.

g) Olibanum extract group rat skin tissue conditions were poor: (1) the epidermis layer has excessive keratinization, the stratum corneum and the granular layer are obviously thickened, the acantha layer is hypertrophic, and the epidermis is widened and prolonged; (2) the dermis layer sees more erythrocytes that are infiltrated and overflowed by inflammatory cells; (3) the skin tissue pathology scores (3.5+ -0.3; P < 0.01) were slightly higher than in the model group.

h) Whereas the skin tissue of the bletilla polysaccharide group performs better among the three groups: (1) the epidermis layer is clear and complete, the skin cuticle is thinner than the model group, and the keratinized substance is slightly thickened; (2) the dermis layer is infiltrated by a small amount of neutrophils; (3) its skin histopathological score (6.7.+ -. 0.2; P < 0.001) is significantly greater than in the model group.

Example 6 Effect of the skin oils (1) and (2) prepared in examples 1 and 2 on the itching model of the combination of histamine and 4-aminopyridine

SPF grade KM mice of 6 weeks old were male in 45 animals (supplied by Liaoning long Biotechnology Co., ltd.). The free drinking water diet is adopted, the temperature is kept at 25+/-1 ℃ during the feeding period, and the bright-dark period is 12 hours.

1) Grouping modeling and drug delivery

Healthy SPF grade KM mice were divided into 45 randomly equally into 9 groups: blank Control (Control), model (Model), skin oil (1) (SFO 1), skin oil (2) (SFO 2), rhizoma Bletillae extract (Bletilla strata), olibanum extract (Frankinense), rhizoma Bletillae polysaccharide (BSP), dexamethasone acetate cream (DAMS), and QINGQINGPENG ointment (QP). After the mice are adaptively raised for 7 days, the hairs of the back of the neck and the ears of the mice are roughly cut off by a shaver closely to the skin, the size of the area where the skin is exposed is about 1.0cm in diameter, and the hair is dehaired again by using dehairing paste; immediately and thoroughly washing with warm water after dehairing to expose the skin of the experimental part for later use; after 1 day of dehairing, recording the frequency of pruritus before administration of each group of mice by video recording; after depilation for 2 days, all mice of the remaining groups were subcutaneously injected with histamine (2 mg/ml,50 μl) and 4-aminopyridine (0.8 mg/ml,50 μl) except the control group without subcutaneous injection, for inducing the behavior of the mice with scrapings.

In the modeling process, animals of a model group, a skin oil (1) group, a skin oil (2) group, a bletilla striata extract group, a frankincense extract group, a bletilla striata polysaccharide group, a compound dexamethasone acetate cream group, and a Qixing Qingpeng ointment group are injected with histamine (2 mg/ml,50 μl) and 4-aminopyridine (0.8 mg/ml,50 μl) by subcutaneous injection respectively, and are immediately placed in a transparent observation box for observation for 30min, and the pruritus latency and the total number of pruritus are recorded by a digital video camera. In the second injection of the molding agent, each of the other groups except the control group and the model group was shaved Mao Buwei and smeared with skin-care oil (1), skin-care oil (2), bletilla striata extract, olibanum extract, bletilla striata polysaccharide (1 mg/ml; bletilla striata polysaccharide solution prepared in example 3), compound dexamethasone acetate cream (Huarun Sanjiu medical Co., ltd.), QINGZHENGQINGGAN ointment (Tibet Qizhen Tibetan medical Co., ltd.) for 4 days each 0.25ml, 1 time per day, until the experiment was completed.

2) Behavioural observations of itch and itch scoring in test mice

The severity of itch between groups was observed by the number of behavioral itches in the mice. Immediately after injecting histamine and 4-aminopyridine subcutaneously into the back of the mouse neck, the mouse was placed in a transparent observation cage and photographed in an unmanned quiet environment for 30min, and the number of scratching behaviors of the mouse was recorded. The shaved area was scraped with the mouse lifting the hind paw and placing the paw back on the floor or mouth until stopped, designated 1 scruff (continuous scruff also designated 1). The frequency of pruritus was recorded for each group of mice (fig. 5).

3) HE staining and pathological analysis of wound tissues of mice tested

After 4 days of administration (i.e., the day of the end of the experiment), all the animals were sacrificed by anesthesia, the skin tissue at the wound site was removed, wrapped with gauze, and fixed in formalin solution for 24 hours. The skin tissue was then sequentially subjected to running water rinsing, dehydration, paraffin embedding, followed by tissue section, HE routine staining, and observation under an optical microscope, and pathological scoring was performed on the skin tissue (fig. 6).

4) Experimental results

(1) Case analysis of itchy mice

The number of 30min scrubs in the transparent observation cage of mice that had been itched by the combination of histamine and 4-aminopyridine was recorded for 6 time periods (1 day before administration of the modeling, on the day of administration of the modeling alone, 1 day after administration of the therapeutic agent, 2 days after administration of the therapeutic agent, 3 days after administration of the therapeutic agent, and 4 days after administration of the therapeutic agent) to be performed.

a) From fig. 5A, it can be seen that the experimental modeling scheme is very successful, and the frequency of the pruritus of each group of mice is remarkably improved after the modeling agent is administered on the modeling day, and the frequency of the pruritus is generally within the range of 30-40 times within 30 minutes. The number of scrapings of the blank groups after 1, 2, 3 and 4 days of administration did not change significantly (1.2.+ -. 0.3, 1.4.+ -. 0.2, 2.0.+ -. 0.5, 1.6.+ -. 0.4, respectively);

b) The number of scrapings was significantly increased in model mice after 1, 2, 3, 4 days post-dose compared to the blank (37.8±1.8, p <0.001, respectively; 36.6+ -1.8, P <0.001; 35.0+ -1.3, P <0.001;34.2 + -1.5, P < 0.001). The frequency of itching remained relatively stable in the blank and model mice during the experiment, indicating that the present modeling protocol was successful.

c) Compared with the model group, the number of scrapings of mice in the skin oil (1) group after 1, 2, 3, 4 days after administration (23.0±1.8, p <0.001, respectively; 12.6+/-3.0, P <0.001; 14.0+ -1.3, P <0.001; 9.6+ -1.0; p < 0.001) showed a significant decrease with the passage of time of administration, with a significant difference. It can be seen that the skin oil (1) has excellent therapeutic effect on itch caused by the combination of histamine and 4-aminopyridine.

e) Compared with the model group, the number of scrapings of mice in the skin oil (2) group after 1, 2, 3, and 4 days after administration (33.5±1.0, respectively; 15.2+/-1.0, P <0.001; 10.2+/-2.3, P <0.001;13.5±0.5, p < 0.001) also exhibited a significant decrease with the passage of the administration time, and there were significant differences after 2, 3, and 4 days after the administration. The results show that the skin oil (2) has good therapeutic effect on itch caused by the combined use of histamine and 4-aminopyridine.

f) Compared with the model group, the number of the scrapings of the mice of the compound dexamethasone acetate cream group after 1, 2, 3 and 4 days after administration (28.6+/-3.1, P <0.01 respectively; 16.0 + -0.7, P <0.001; 15+/-1.3, P <0.001;16.4±2.3, p < 0.001) also showed a significant decrease with the passage of the administration time, also with a significant difference. The results show that the compound dexamethasone acetate emulsifiable paste also has good therapeutic effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

g) Compared with the model group, the number of scrapings of mice in the Qixing Qingpeng ointment group after 1, 2, 3 and 4 days after administration (25.8+/-0.9 and P <0.001 respectively; 22.8+/-1.4, P <0.001;21.0 + -2.2, P <0.001;17.3±2.0, p < 0.001) also showed a significant decrease with the passage of the administration time, with a significant difference, suggesting that the qingpeng ointment also showed a better therapeutic effect on itching caused by the combination of histamine and 4-aminopyridine.

The improvement effect of the four medicines on the pruritus frequency is compared, and the Qingpeng ointment, the compound dexamethasone acetate emulsifiable paste, the skin oil (1) and the skin oil (2) have good treatment effects, wherein the skin oil (1) has the best effect.

From fig. 5B, it can be seen that the frequency of the scrapings of mice in the bletilla striata polysaccharide (bletilla striata polysaccharide solution prepared in example 3), the bletilla striata extract and the olibanum extract all exhibited a degree of decrease with the lapse of the administration time.

h) Compared with the model group, the number of the scrapings of the bletilla striata polysaccharide group mice after 1, 2, 3 and 4 days after administration (37.4+/-1.9 respectively; 20.4+/-1.5, P <0.001; 23.8+ -3.9, P <0.01;22.2±1.3, p < 0.001) also exhibited a significant decrease with the passage of the administration time, and there were significant differences after 2, 3, and 4 days after the administration. The results show that the bletilla striata polysaccharide has a certain effect of relieving itching caused by the combined use of histamine and 4-aminopyridine, but the effect is not obvious.

i) Compared with the model group, the number of times of the scrapings of the mice in the bletilla striata extract group after 1, 2, 3 and 4 days after administration (30.8+/-1.9 respectively; 28.6+/-1.8; 22.2+/-2.4, P <0.001;20.8±1.8, p < 0.001) also exhibited a significant decrease with the passage of the administration time, and there was a significant difference after 3, 4 days after the administration. The results show that the bletilla striata extract has a certain treatment effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

j) Compared with the model group, the number of scrapings of mice in the olibanum extract group after 1, 2, 3, and 4 days of administration was (19.2±0.8, p <0.001; 18.6+ -1.6, P <0.001; 22.6+/-3.9, P <0.001;21.4±2.0, p < 0.001) also showed a significant decrease with the passage of the administration time, with a significant difference; the olibanum extract also has certain therapeutic effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

Comparing the above three medicines with each other, the Olibanum extract has the best effect of relieving the frequency of the pruritus.

(2) Results of histopathological experiments

The pathological analysis results of all mice after cervical spine removal and sacrifice (after 4 days of molding) and HE staining of the affected skin are shown in FIG. 6.

a) As can be seen from fig. 6A, the epidermis layer of the mice in the blank group is complete in structure, and the epithelial cells are arranged in polarity; the subcutaneous observation of numerous hair follicles and other accessory organs, without abnormal pathological changes such as oedema and inflammatory cell nest infiltration.

b) Skin of the mice in the model group at the site of scrapings compared to the blank group: (1) the epidermis layer is partially deleted and has disordered structure, and the epidermis layer is obviously thickened; (2) the dermis layer is visibly marked with edema and massive inflammatory cell nest infiltration; (3) the pathological scores of the skin tissues (2.0+ -0.3, P < 0.001) were significantly less than the blank (9.0+ -0.3).

c) Skin appearance of the skin-care oil (1) group mice with the skin at the site of the scrapings compared to the model group: (1) the epidermis layer is complete in structure, and the epithelial cells are arranged in a polar manner, so that obvious thickening is not seen; (2) dermis is seen with follicular hyperplasia and small inflammatory cell infiltrates; (3) its skin histopathological score (7.6.+ -. 0.2; P < 0.001) was significantly higher than that of the model group.

d) Skin appearance of skin-friendly oil (2) group mice with the scrapings compared to model group: (1) the epidermis layer has complete structure, the thickness is equivalent to that of the blank group, and the epithelial cells are arranged in polarity; (2) the dermis layer can be seen with a large number of follicular hyperplasia and a small number of inflammatory cell infiltrates; (3) its skin histopathological score (7.0.+ -. 0.5; P < 0.001) was significantly higher than that of the model group.

e) Compared with the model group, the skin of the mouse with the dexamethasone acetate compound cream group shows that: (1) the epidermis layer is complete in structure and slightly thickened; (2) dermis layers can be seen with follicular hyperplasia and mild oedema and a small inflammatory cell infiltration; (3) its skin histopathological score (6.8.+ -. 0.3; P < 0.001) was significantly higher than that of the model group.

f) Skin presentation at the mouse scrapings site in Qingpeng ointment group compared to model group: (1) the epidermis layer is complete in structure and slightly thickened; (2) the dermis layer is seen with a small number of hair follicle hyperplasia and a small number of inflammatory cell infiltrates; (3) its skin histopathological score (5.4.+ -. 0.2; P < 0.001) was significantly higher than that of the model group.

g) As can be seen from fig. 6B, the skin of the mice with the mastic extract group exhibited the skin of the scrapie area in the model group control: (1) the epidermis layer is complete in structure and slightly thickened, and the epithelial cells can be arranged; (2) the dermis layer is visible with edema and focal inflammatory cell infiltration; (3) its skin histopathological score (5.6±0.2, p < 0.001) was significantly higher than that of the model group.

h) Skin appearance of mouse scrapings of bletilla striata extract group compared to model group: (1) the epidermis layer is complete in structure and slightly thickened, and the epithelial cells can be arranged; (2) the dermis layer can be seen to proliferate in the skin appendages such as hair follicles; (3) its skin histopathological score (4.6.+ -. 0.2, P < 0.001) was higher than that of the model group.

i) Compared with the model group, the bletilla striata polysaccharide (bletilla striata polysaccharide solution prepared in example 3) group showed skin appearance of the mouse scrapie part: (1) the epidermis layer structure part is missing, (2) the dermis layer can be seen to proliferate skin appendages such as hair follicles and the like and infiltrate focal inflammatory cells; (3) its skin histopathology score (3.4±0.2, p < 0.01) was higher than that of the model group. Example 7 skin oil preparation prepared in example 1 (i.e., topical skin oil (1)) typical cases one (1) treatment method for perineal eczema

The primary common cold of the age of 81 has the defect of incomplete urination, and the residual urine leaks wet underpants easily, which results in the perineum eczema for more than ten years. In the period, local skin is scratched due to itching, so that the doctor can accept diagnosis and treatment in certain people hospitals in Yueyang city, and the doctor can quickly relieve the doctor after the compound dexamethasone acetate emulsifiable paste is smeared. Half a year ago, the treatment is admitted due to the complication of the perineal eczema by cerebral infarction. During hospitalization, western hormone ointments are still accepted, but the effect is not obvious, and attempts to use traditional Chinese medicine rehabilitation therapy and voluntarily try skin oil are recommended. After the affected part is rinsed with the help of family, a proper amount of skin-growing oil (1) prepared in example 1 is smeared on the perineum part by a thin layer by using a clean cotton swab, the wet state of the affected part is kept for not less than 30 minutes after each smearing, and each time of 1 day in the morning and at night, a treatment course is that the smearing is maintained until the healing is achieved. The affected area was inspected daily and photographed for records.

(2) Therapeutic results

After the patient uses the skin-care oil (figure 7), the itching at the perineum is obviously relieved, and the polymorphic red lesions and inflammatory exudation of the affected parts are quickly inhibited. Transfer from family members: after 10 days of application, the patient basically does not scratch the affected part of the perineum, does not have exudates, and the skin color of the affected part is close to normal. As the years of the Mr. are high and the patient often suffers from enuresis, the family members are recommended to be smeared with at least 1 secondary skin oil every day as much as possible, so that the recurrence of eczema is prevented.

Example 8 skin oil preparation prepared in example 1 (i.e., topical skin oil (1)) typical case two for treating eczema at cheek

(1) Therapeutic method

The first two days of the female with the age of 40 years, the right cheek is suddenly soaked with water and is itchy, and the skin of the affected part is scratched when the female is hard to wear. Then, the department of dermatology (the important specialized department of Wuhan, the national traditional Chinese and Western medicine combines the important specialized department, the national dermatology "four-strength") in the X-th hospital of Wuhan is diagnosed as acute eczema by a doctor of a certain principal of famous doctors, and the following medicines are taken orally and externally used for treatment:

triamcinolone acetonide econazole cream (10 g/min), olopatadine hydrochloride tablet (10 tablets/box) 2 boxes, common selfheal fruit-spike tablet (36 tablets/box) 2 boxes, erodii seu Geranium ointment (15 g/min), ganlin lotion (150 ml/bottle).

After two days of using the medicine according to the doctor's advice, symptoms such as red and swollen cheek skin, itching and pain, blisters and exudation are not obviously reduced. Then voluntarily try to wipe the skin oil and automatically stop the oral and external medicines in the hospital. Before the skin oil is smeared, the affected parts of the cheeks are rinsed with clear water, a proper amount of the skin oil (1) prepared in the embodiment 1 is taken by a clean cotton swab after air drying, and is smeared in a thin layer (the affected parts are smeared for 1 time before sleeping in practice, and the cheeks of the affected parts sleep upwards for one night, so that the skin oil is not smeared).

(2) Therapeutic results

The next day after the application of the skin-growing oil (1) (i.e. 10 hours after application; fig. 8), the von women first had a feeling of consciously touching the cheek-affected part that had no itching and pain; when the affected part was observed to be pale red when the mirror was taken, the swelling was substantially resolved, the blister was also disappeared, and only a small amount of crust was present (right panel). It is recommended to apply it for two days again to consolidate the effect.

Claims

1. The application of the external skin oil in preparing the medicine for preventing and treating eczema is characterized in that the external skin oil is prepared from the following raw materials in parts by weight:

30-40 parts of astragalus membranaceus, 30-40 parts of angelica sinensis, 30-40 parts of gynura procumbens, 30-40 parts of garden burnet, 15-20 parts of frankincense, 15-20 parts of bletilla striata, 15-20 parts of angelica dahurica, 15-20 parts of cynanchum atratum, 10-20 parts of myrrh, 10-20 parts of scutellaria baicalensis, 10-20 parts of coptis chinensis, 10-20 parts of phellodendron amurense, 10-20 parts of rheum officinale, 3-5 parts of borneol and 1000-1500 parts of edible vegetable oil;

The edible vegetable oil is at least one selected from sesame oil, peanut oil, coconut oil, soybean oil, linseed oil, castor oil and olive oil;

the preparation method of the external skin care oil comprises the following steps: placing edible vegetable oil into a container, and adding dry powder of radix astragali, radix Angelicae sinensis, radix Gynostemmatis, radix Sangusorbae, rhizoma Bletillae, radix Angelicae Dahuricae, radix Cynanchi Atrati, scutellariae radix, radix et rhizoma Rhei, coptidis rhizoma, cortex Phellodendri, olibanum and Myrrha; placing the container for containing the above medicinal materials in colloid mill, and circularly grinding at room temperature for 2-5 h; and (3) after stopping, shearing at a high speed, sieving with a 5000-mesh sieve under a negative pressure to remove residues, collecting filtered oil, adding borneol into the filtered oil, fully stirring, and cooling to obtain the external skin care oil.

2. The application of claim 1, wherein the external skin care oil is prepared from the following raw materials in parts by weight:

40 parts of astragalus, 40 parts of angelica, 40 parts of gynura procumbens, 40 parts of garden burnet, 20 parts of frankincense, 20 parts of bletilla striata, 20 parts of angelica dahurica, 20 parts of cynanchum atratum, 20 parts of myrrh, 10 parts of radix scutellariae, 10 parts of coptis chinensis, 10 parts of cortex phellodendri, 10 parts of rheum officinale, 5 parts of borneol and 1500 parts of edible vegetable oil.

3. The application of claim 2, wherein the external skin care oil is prepared from the following raw materials in parts by weight:

30 parts of astragalus, 30 parts of angelica, 30 parts of gynura procumbens, 30 parts of garden burnet, 15 parts of frankincense, 15 parts of bletilla striata, 15 parts of angelica dahurica, 15 parts of cynanchum atratum, 10 parts of myrrh, 20 parts of scutellaria baicalensis, 20 parts of coptis chinensis, 20 parts of phellodendron amurense, 20 parts of rheum officinale, 3 parts of borneol and 1185 parts of edible vegetable oil.

4. A use according to any one of claims 1-3, wherein the high speed shearing step is: the flash extraction device shears 3-5 times at 20000 rpm for 5 min each time.

5. Use according to any one of claims 1-3, wherein the eczema is caused by a sensitization and/or inflammation causing drug.

6. Use according to any one of claims 1 to 3, wherein the eczema is acute eczema of the cheek or chronic eczema of the perineum.