CN115844992A

CN115844992A - External skin-generating oil for preventing and treating eczema and pruritus as well as preparation method and application thereof

Info

Publication number: CN115844992A
Application number: CN202211585608.6A
Authority: CN
Inventors: 唐和斌; 马巳媛; 谭港宁; 张朝; 张君月; 杨贵淳; 唐晟杰; 李玉桑
Original assignee: South Central University for Nationalities
Current assignee: South Central Minzu University
Priority date: 2022-12-09
Filing date: 2022-12-09
Publication date: 2023-03-28
Anticipated expiration: 2042-12-09
Also published as: CN115844992B

Abstract

The invention relates to the technical field of new drug indications, and particularly discloses external skin-generating oil for preventing and treating eczema and pruritus as well as a preparation method and application thereof. The external skin-generating oil is prepared by mixing the following raw materials in parts by weight through a colloid mill, a flash extraction mode and the like: 30-40 parts of astragalus membranaceus, 30-40 parts of angelica sinensis, 30-40 parts of gynura procumbens, 30-40 parts of garden burnet, 15-20 parts of frankincense, 15-20 parts of bletilla striata, 15-20 parts of angelica dahurica, 15-20 parts of cynanchum atratum, 10-20 parts of myrrh, 10-20 parts of scutellaria baicalensis, 10-20 parts of coptis chinensis, 10-20 parts of golden cypress, 10-20 parts of rheum officinale, 3-5 parts of borneol and 1000-1500 parts of edible vegetable oil. Scientific experiments prove that the external skin-regenerating oil preparation has better effects of tonifying qi and nourishing blood, activating blood and removing stasis, purging fire and detoxifying, removing putrefaction and promoting tissue regeneration, dispelling wind and relieving itching, can promote the quick elimination of acute and chronic eczema symptoms of a rat induced by DNFB in a short time, and can also quickly inhibit the itching signs of the rat induced by the combination of histamine and 4-aminopyridine.

Description

External skin-generating oil for preventing and treating eczema and pruritus as well as preparation method and application thereof

Technical Field

The invention relates to the technical field of new drug indications, in particular to external skin-generating oil for preventing and treating eczema and pruritus as well as a preparation method and application thereof.

Background

Eczema is characterized by localized skin rash and exudation tendency or infiltration and pachynsis of epidermis and dermis caused by various internal factors (such as chronic digestive system diseases, endocrine dyscrasia or mental stress, insomnia, overfatigue, mood changes and the like) and external factors (such as environment, climate changes, sunlight, various animal furs, plants, cosmetics and the like), and is accompanied by severe pruritus and skin diseases which are easy to repeatedly attack. The incidence of eczema is the highest of all skin disorders. Meanwhile, the eczema can cause skin injury, and acute eczema generates large-area skin injury and herpes simplex and has exudation tendency; the skin of the affected part of the chronic eczema is obviously thickened, the moss-like change appears, and the moss-like change is easy to happen repeatedly, which greatly influences the daily work and life of the patient.

Currently, western medicine clinical classification of eczema as allergic diseases and treatment principles of relieving itching as soon as possible, controlling skin inflammation, and recovering skin barrier function, thereby improving the quality of life of patients. The western medicine mostly adopts an internal and external concurrent therapy method, patients are usually advised to take oral antihistamines such as chlorpheniramine, cetirizine, loratadine, olopatadine hydrochloride, terfenadine and the like, and are combined with topical glucocorticoids (such as prednisone, compound dexamethasone acetate cream and the like) and calcium-modulated nerve phosphate inhibitors and calcium preparations (calcium lactate, calcium gluconate and the like) of non-hormone treatment drugs, or are matched with topical external washing such as calamine lotion (used when no exudate is generated at first) and 3% boric acid solution (used when much exudate or erosion occurs). Although the therapy and the drugs are simple to use, the clinical actual treatment effect is not ideal, adverse reactions such as skin atrophy, pigmentation and the like can be caused by long-term repeated use, and the adverse reactions are easy to relapse after the drug is stopped, even endocrine metabolism disorder is caused, and the better control is difficult.

The traditional Chinese medicine considers that eczema belongs to the category of eczema and is caused by the fact that damp evil is accumulated to transform into heat and then the damp-heat is soaked in the skin. Traditional Chinese medicine doctors in the past generations of China mostly consider that the traditional Chinese medicine is formed by that people have intrinsic weakness and insufficient vital qi, and have exogenous wind, dampness and heat pathogens to invade and deposit on skin for a long time to cause skin malnutrition: (1) Ancient Chinese doctors believe that the etiology and pathogenesis of eczema mainly comprise congenital deficiency, spleen qi weakness, external wind, dampness, heat and the like; damage to the heart and spleen; itching refers to excessive heart fire and white scurf refers to spleen qi deficiency; (2) Modern Chinese medicine doctors believe that the disease is caused by congenital deficiency, fetal toxin, fever and heat stagnation on skin; or the spleen fails to transport and transform due to unbalanced postnatal feeding and irregular diet, and then the skin is infected with wind, heat, dampness and toxin, and pathogenic toxin is accumulated in the skin; repeated attack, prolonged period of time or emotional internal injury, which results in spleen deficiency and qi and blood generation and source depletion, yin blood and primordial qi depletion, blood deficiency and wind dryness, and malnutrition of the skin. Therefore, in terms of treatment, some medicines for clearing heat and removing toxicity with convergence are selected: phellodendron bark, flavescent sophora root, purslane, gromwell, wild chrysanthemum flower, etc.; when the exudation is not too much, the powder is selected for external application, such as indigo naturalis powder; due to the change of fleshy moss on the skin in the chronic period, medicaments can be selected on the basis of using the skin moistening cream, and Chinese patent medicaments (such as a ganlin lotion) or a self-prepared fumigation lotion, an emulsion or an ointment are selected, so that heat clearing, dampness removing and itching relieving substances are selected in the treatment process. But it is often difficult to control symptoms in time, effectively and quickly for an acute episode of eczema.

So far, no matter in western medicine or traditional Chinese medicine, aiming at the characteristics of complicated causes, multiple syndromes and easy repetition of eczema in clinic, a specific medicine which is simple and convenient to use, has no toxic or side effect, and has quick and obvious curative effect, especially an external medicine, is still lacked. Therefore, the active search for a drug which can reduce inflammatory cell recruitment and promote skin barrier function recovery, and an effective drug suitable for treating various types of eczema, and the search for a breakthrough eczema treatment method become the key points of attention of many medical researchers, and are also the main purposes of the invention.

Disclosure of Invention

Aiming at the problem that the prior art lacks special drugs for preventing and treating eczema and pruritus:

the first purpose of the invention is to provide the external skin-growing oil for effectively preventing and treating eczema and pruritus.

The second purpose of the invention is to provide a preparation method of the external skin-growing oil for preventing and treating eczema and pruritus.

The third objective of the invention is to provide the medical application of the external skin-generating oil for preventing and treating eczema and pruritus.

The invention concept of the invention is as follows:

eczema is a skin disease which is characterized by localized and multiple rashes, exudation tendency or infiltration and pachynsis of epidermis and dermis superficial layer caused by various internal factors (such as chronic digestive system diseases, endocrine dyscrasia or mental stress, insomnia, overfatigue, mood changes and the like) and external factors (such as environment, climate changes, sunlight, various animal fur, plants, cosmetics and the like), is accompanied by severe pruritus and is easy to repeatedly attack. Of all skin diseases, the incidence of eczema is the highest.

According to the age of the ill people, it can be divided into children eczema and adult eczema. The development of eczema usually begins in infancy. Statistically, the incidence of eczema in children under 12 years of age is high, reaching approximately 30% in developed countries. The incidence of eczema in adults is lower than that in children, but is close to 10%, and the prevalence rate of eczema in developing countries tends to be increased. There are approximately 1 million patients with eczema in china, calculated as 10% morbidity, which is undoubtedly a very large number. Once the children's acute eczema is treated improperly or is not cured for a long time, the acute eczema is worsened in the adult stage, and more vexing symptoms such as intractable pruritus, pain, infection and the like are caused.

Eczema can be classified into three types of acute, subacute and chronic types according to the course of disease and corresponding pathological features (exudation or infiltration and hypertrophy). Generally speaking, acute eczema is better within 6 weeks on the premise of timely and correct treatment. If the persistence is not long, the disease course of children is considered as chronic eczema for more than 3 months, while the disease course of adults is considered as chronic eczema for more than half a year.

From the pathological changes of local skin tissue structure, the affected epidermis of the patient with acute eczema is spongy and edematous, blisters are formed in the acanthosphere and under the horny layer, and local lymphocyte and neutrophil infiltration can be seen; it has small dermal superficial vasodilatation and mild inflammatory cell infiltration around the blood vessels, mainly of lymphocytes. Once the epidermis is degraded into subacute or even chronic eczema, the epidermis is thickened, hyperkeratosis and obvious spongiform edema, inflammatory cell infiltration around small vessels of the superficial dermis, the number of capillaries is increased, and even endothelial cell swelling and hyperplasia and other abnormal changes occur.

At present, the clinical prevention and treatment of eczema in western medicine is based on the treatment principle of relieving pruritus, controlling skin inflammation and recovering the skin barrier function as soon as possible so as to improve the life quality of patients. The western medicine mostly adopts an internal and external concurrent therapy method, and patients are often advised to take antihistamines such as chlorpheniramine, cetirizine, loratadine, terfenadine and the like orally, and are combined with a calcineurin inhibitor and a calcium preparation of external glucocorticoid and non-hormone treatment medicines, or are matched with a calamine lotion, a 3% boric acid solution and the like for local external washing. Although the therapy and the drugs are simple to use, the actual clinical treatment effect is not ideal, even various adverse reactions are generated, and the disease condition is easy to relapse and difficult to control.

In relation to eczema, ancient physicians in China named "impetigo", "crural sore", "hydrangea wind", "wind of four bends", "psoriasis", etc. according to the difference of the symptoms and the parts of the disease, and these descriptions are similar to acute eczema, ear Zhou Shizhen, scrotal eczema, atopic dermatitis, infantile eczema, etc. in western medicine. Although the cause of eczema is complex and the influence factors are more, traditional Chinese medicine doctors in the past generally think that the eczema is formed by skin malnutrition caused by intrinsic weakness, insufficient vital qi, invasion of wind, dampness and heat evil and long-term deposit on the skin. The syndrome differentiation and classification of the liver-heat-type traditional Chinese medicine are also common in damp-heat syndrome, blood-heat syndrome, spleen deficiency and damp accumulation, blood deficiency and wind dryness and the like, and the liver-heat-clearing decoction with gentian, the blood-cooling and dampness-removing decoction, the spleen deficiency and dampness-accumulation-benefiting dampness-removing stomach-poria decoction, the angelica drink, and the like which are good at enriching blood and dispelling wind are respectively added and modified for oral administration along with the syndrome.

In "Jingyue quan Shu", the Ming Dynasty doctor Zhang Jingyue mentions: for swollen carbuncle-abscess sharp shovel with red pain, although the internal treatment method has been previously done, the decoction is slow and the pain is difficult to treat, and it is necessary to apply the external application. Xulingtai (a clear famous doctor) also has cloud in the written "medical source flow theory and enclosed medicine theory" (the cloud is the term for Qing dynasty): the most important of surgical methods is external treatment, especially for external treatment. Therefore, according to the characteristics of the eczema with superficial position and exposed focus, the traditional Chinese medicine is selected for external application to directly reach the focus, and has the effects of penetrating the skin, dredging the channels and collaterals, promoting blood circulation and removing blood stasis, thereby achieving the curative effects of eliminating pathogenic factors, strengthening the body resistance and repairing the skin barrier. The external treatment method of traditional Chinese medicine is used as the characteristic and the advantage of the traditional Chinese medicine, and really achieves better curative effect in the aspect of treating eczema.

Of course, it is known from the definition of eczema that skin itching is one of the important clinical manifestations, and more seriously affects the quality of life of patients, and the most important signs of timely and effective treatment are urgently needed.

With the scientific insight into the neuroanatomy and neurophysiology of pruritus, the pruritus research international forum has formulated and promulgated a standardized two-step clinical classification of pruritus: (1) based on the first step of clinical presentation. It includes: inflammatory dermatosis-induced pruritus, non-inflammatory dermatosis-induced pruritus and pruritus related to chronic scratching secondary skin lesion; (2) a second step based on underlying disease. It comprises the following steps: skin disorders, systemic disorders, neurological disorders, psychiatric and psychological disorders, mixed and other uncertain etiologies. However, since the scientific research and theoretical cognition on pruritus are relatively delayed after the start, the current theories are still in the stage of perfection, and various phenomena related to pruritus cannot be comprehensively explained. Despite the existing therapeutic guidelines for pruritus, the clinical practice still lacks effective individualized (often considering patient age, underlying disease, past history, pruritus severity and effect on patient quality of life) treatment strategies due to the truly complex etiology and multiple factors that cause pruritus.

For the treatment of pruritus, western medicine clinical emphasis is first placed on avoiding allergen exposure, disabling suspected drugs and effectively treating the onset of the allergen, while fighting with antihistamine H ₁ And H ₂ Receptor antagonist, glucocorticoid (for children, no standard scheme for external glucocorticoid exists), immunoregulation and the like are mainly adopted, but adverse reaction often occurs, so that pruritus can quickly recur after treatment intermission.

According to accompanying symptoms, secondary skin damage, attack seasons and the like, the traditional Chinese medicine considers that skin itch initially belongs to wind and enters blood after a long time, and the skin itch is divided into two types of wind syndrome and blood syndrome. Wherein, wind syndrome can be re-typed according to the dry and wet physical signs: if the skin is dry and lichen is wind-dry, the prescription should be added with dryness-moistening and pathogenic wind-expelling medicines such as fructus Tribuli, periostracum Cicadae, semen Sesami, and pollen; for example, eczema-like skin wet rot is a kind of rheumatism, and it is advisable to take herbs for removing dampness and clearing heat such as Ku Shen, huang Bai, di Fu Zi and Bai Xian Pi. The blood system syndrome is subdivided again by the syndrome of blood heat and blood deficiency: the young and middle-aged patients usually have blood heat as the main syndrome, and the treatment should follow the principle of cooling blood and clearing heat; the elderly patients mainly have blood deficiency, and should be treated by nourishing blood and moistening skin. If the prolonged unhealed blood stasis results in obstruction of collaterals, the treatment principle of activating blood and resolving stasis should be considered, and it can be modified with Taohong Siwu Tang.

Based on clinical experience of decades and scientific research accumulation of pharmacology of Chinese medicines, the inventor of the technology thinks that the common pathogenesis system of eczema and pruritus is formed by intrinsic weakness, insufficient vital qi, invasion of exogenous wind, dampness and heat evil, long-term accumulation on skin, unsmooth qi and blood, skin malnutrition caused by the invasion of exogenous wind, dampness and heat evil, and skin ulcer after long-term rupture. Therefore, the inventor of the technology provides unique prevention and treatment insights aiming at the pathogenesis of eczema and pruritus, namely, the Chinese herbal medicine adopts the products of tonifying qi and activating blood circulation, removing blood stasis and dredging collaterals, healing sore and promoting granulation, dispelling wind and relieving itching, clearing heat and cooling blood, eliminating dampness and detoxifying to play the roles of detoxifying and removing putrefaction, and promoting the growth and treatment of sore muscles so as to quickly eliminate the suffering from eczema and pruritus. The inventor of the technology has revealed in earlier work that the external skin-growing oil for treating skin burns and scalds conforms to the medication principle and has the presumed effect. Therefore, the applicant speculates that the external skin-generating oil can also effectively cure the eczema and pruritus diseases, and adds or subtracts components on the basis of the original formula, and adjusts the dosage of the reserved medicine according to the treatment principle proposed by the applicant to form the formula of the external skin-generating oil for preventing and treating the eczema and the pruritus.

Therefore, the applicant carries out a large number of scientific experimental examinations on the prevention and treatment of eczema and pruritus of the skin oil, and researches the treatment effect of the prepared skin oil on diseases through external local administration by respectively establishing animal models of acute eczema, chronic eczema and pruritus and clinically treating acute eczema of cheek and chronic eczema of perineum. Research results prove that the skin-generating oil prepared by the method can promote acute and chronic eczema symptoms of rats induced by Dinitrofluorobenzene (DNFB), can quickly inhibit itching signs of mice induced by combined medication of histamine and 4-aminopyridine, and can quickly promote the repair of skin tissue structures of wound surfaces of animals of various models; the prevention and treatment effects of the ointment are obviously better than those of the control medicines (1) anti-inflammatory western medicines, namely the externally applied compound dexamethasone acetate cream, and (2) Chinese medicines with the effects of clearing heat and drying dampness, promoting blood circulation and dispelling wind, relieving itching and diminishing inflammation, namely the externally applied compound phellodendron liquid, ice Huang Fule ointment and Qizhengqingpeng ointment). Moreover, the skin oil preparation of the invention has obviously better effect on acute eczema of cheeks and chronic eczema of perineum than the western medicine or the Chinese and western medicine combined medicine of famous doctors in the famous hospital (such as the combined medicine of triamcinolone acetonide econazole cream, olopatadine hydrochloride tablets, prunella grass tablets, dingrou geranium ointment and Ganling lotion).

In order to achieve the first object, the invention adopts the following technical measures:

the external skin-generating oil for preventing and treating eczema and pruritus is prepared from the following raw materials in parts by weight: 30-40 parts of astragalus membranaceus, 30-40 parts of angelica sinensis, 30-40 parts of gynura procumbens, 30-40 parts of garden burnet, 15-20 parts of frankincense, 15-20 parts of bletilla striata, 15-20 parts of angelica dahurica, 15-20 parts of cynanchum atratum, 10-20 parts of myrrh, 10-20 parts of scutellaria baicalensis, 10-20 parts of coptis chinensis, 10-20 parts of golden cypress, 10-20 parts of rheum officinale, 3-5 parts of borneol and 1000-1500 parts of edible vegetable oil;

the edible vegetable oil is selected from at least one of sesame oil, peanut oil, coconut oil, soybean oil, linseed oil, castor oil and olive oil, preferably at least one of sesame oil and coconut oil.

Preferably, the external skin-generating oil for preventing and treating eczema and pruritus is prepared from the following raw materials in parts by weight: 40 parts of astragalus membranaceus, 40 parts of angelica sinensis, 40 parts of gynura procumbens, 40 parts of garden burnet, 20 parts of frankincense, 20 parts of bletilla striata, 20 parts of angelica dahurica, 20 parts of cynanchum atratum, 20 parts of myrrh, 10 parts of scutellaria baicalensis, 10 parts of coptis chinensis, 10 parts of golden cypress, 10 parts of rheum officinale, 5 parts of borneol and 1500 parts of edible vegetable oil.

Preferably, the external skin-generating oil for preventing and treating eczema and pruritus is prepared from the following raw materials in parts by weight: 30 parts of astragalus membranaceus, 30 parts of angelica sinensis, 30 parts of gynura procumbens, 30 parts of garden burnet, 15 parts of frankincense, 15 parts of bletilla striata, 15 parts of angelica dahurica, 15 parts of cynanchum atratum, 10 parts of myrrh, 20 parts of scutellaria baicalensis, 20 parts of coptis chinensis, 20 parts of phellodendron amurense, 20 parts of rheum officinale, 3 parts of borneol and 1185 parts of edible vegetable oil.

In order to achieve the second purpose, the invention adopts the following technical measures:

a preparation method of the external skin-growing oil for preventing and treating eczema and pruritus comprises the following steps: putting edible vegetable oil into a container, and adding dry powder of radix astragali, radix Angelicae sinensis, radix Gynurae Divaricatae, radix Sangusorbae, olibanum, rhizoma Bletillae, radix Angelicae Dahuricae, radix Cynanchi Atrati, myrrha, scutellariae radix, radix et rhizoma Rhei, coptidis rhizoma, and cortex Phellodendri; placing the container containing the above medicinal materials in a colloid mill, and circularly grinding at normal temperature for 2-5 hr; and (3) after stopping, shearing at a high speed of 20000rpm by a flash type extraction device for 3-5 times (5 min each time), filtering with a 5000-mesh sieve under negative pressure to remove residues, collecting filtered oil, adding Borneolum Syntheticum into the filtered oil, and stirring completely to obtain the external skin-regenerating oil for preventing and treating eczema and pruritus.

In order to achieve the third object of the present invention, the present invention further adopts the following technical measures:

the invention also provides application of the external skin-generating oil in preparing medicines for preventing and treating eczema and pruritus.

The eczema and pruritus are caused by sensitizing and/or inflammation-causing medicines.

Preferably, the eczema is acute eczema of cheek or chronic eczema of perineum.

The formula principle of each traditional Chinese medicine raw material of the invention is as follows:

radix astragali, radix Angelicae sinensis, gynura procumbens, radix Sanguisorbae, olibanum, myrrha, etc. have effects of invigorating qi, promoting blood circulation, removing blood stasis, dredging collaterals, cooling blood, and healing sore; bletilla striata, cynanchum atratum, angelica dahurica and the like can eliminate swelling, expel pus, eliminate putridity, promote tissue regeneration, dispel wind and relieve itching and are used as ministers; baikal skullcap root, coptis root, phellodendron bark, rhubarb, etc. can clear heat and purge fire, dry dampness and remove toxicity, and are used as adjuvants; the borneol and the vegetable oil are used for coordinating with the other medicines to achieve the effects of detoxifying and promoting tissue regeneration, dispelling wind and relieving itching.

The selection of the eczema and pruritus models and the index evaluation are as follows:

acute eczema: (1) DNCB-induced model of acute eczema exposure in rats; (2) The first X Hospital dermatology of Wuhan City called the famous medical staff of Wuhan City called the cheek acute eczema case for a certain diagnosis;

chronic eczema: (1) DNCB-induced model of chronic eczema in rats exposed to contact; (2) Senile perineum chronic eczema diagnosed by king medical practitioner in medical department of certain people hospital in Yueyang City;

the pruritus model: a mouse itch model induced by the combination of histamine and 4-aminopyridine;

evaluation indexes are as follows: (1) Gross observation (including scoring) of affected skin in acute and chronic eczema rats; (2) HE staining observation and pathological analysis (including scoring) of the affected part tissues of the acute and chronic eczema rats; (3) Pruritus frequency of pruritus model mice and HE staining observation and pathological analysis (including scoring) of affected tissues.

Compared with the prior art, the invention has the following advantages and effects: research results show that the skin-regenerating oil prepared by the invention can be effectively utilized after being applied for both preventive and therapeutic use to benefit qi and activate blood, remove blood stasis and dredge collaterals, heal sores and promote tissue regeneration, dispel wind and relieve itching, clear heat and cool blood, eliminate dampness and detoxify so as to play the roles of detoxifying and removing putrefaction and regenerating sore muscles and quickly eliminate the suffering of eczema and pruritus. The prevention and treatment effect of the ointment is obviously better than that of contrast medicines commonly used in western medicine and traditional Chinese medicine (1) anti-inflammatory, antibacterial and antiallergic western medicines including compound dexamethasone acetate cream, triamcinolone acetonide econazole cream and olopatadine hydrochloride tablets, and 2) Chinese patent medicines with effects of clearing heat and drying dampness, promoting blood circulation and dispelling wind, relieving itching and diminishing inflammation, including compound phellodendron bark liquid for external use, ice Huang Fule ointment, qizhengqingpeng ointment, dingrou geranium ointment and Ganling lotion).

The skin-growing oil disclosed by the invention has the following advantages for preventing and treating eczema and pruritus: (1) the cost of the medicine is low; (2) the external use is convenient, and the effect is quick; (3) has good rehabilitation effect on acute eczema of cheek and chronic eczema of perineum.

Drawings

FIG. 1 is a graph showing the effect of the tested drugs on the skin of the affected part of acute eczema and the degree of damage. A is the influence and the score chart of raw skin oil (1) (SFO 1), raw skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXMS) and compound phellodendron bark liquid (HBXJ) on the appearance of the affected skin of an experimental rat; b is the influence of Bletilla striata extract (Bletilla striata), frankincense extract (frankiness) and Bletilla Striata Polysaccharide (BSP) on the appearance of the skin of the affected part of the experimental rat and a score chart thereof. Graphs indicate P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scale in the figure is 1 cm.

FIG. 2 is a chart showing the effect of the tested drugs on the pathological structure of the skin of the affected part of acute eczema and the pathological scores thereof. A is the influence of raw skin oil (1) (SFO 1), raw skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXMS) and compound cortex Phellodendri liquid (HBXJ) on pathological tissues of affected skin of experimental rat (HE staining result) and its score chart; b is the influence of Bletilla striata extract (Bletilla striata), frankincense extract (Frankinense) and Bletilla Striata Polysaccharide (BSP) on the pathological tissues of the affected part of an experimental rat (HE staining result) and a score chart thereof. White line segments in the figure represent the thickened dermal tissue epidermis layer of the rat; white arrows indicate inflammatory cell infiltration; white open arrows indicate subcutaneous fibrous tissue; graphs indicate P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scale in the figure is 200 micrometers (objective:. Times.4) and 50 micrometers (objective:. Times.20).

FIG. 3 is a graph showing the effect of the tested drugs on the skin of the affected part of chronic eczema and the degree of damage. A is the influence and the score chart of skin appearance of an affected part of an experimental rat by raw skin oil (1) (SFO 1), raw skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXMS) and ice Huang Fule (BHFL); b is the influence of Bletilla striata extract (Bletilla striata), frankincense extract (frankiness) and Bletilla Striata Polysaccharide (BSP) on the appearance of the skin of the affected part of the experimental rat and a score chart thereof. Graphs indicate P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scale in the figure is 1 cm.

FIG. 4 is a chart showing the effect of the tested drugs on the pathological structure of the skin of the affected part of chronic eczema and the pathological scores thereof. A is the influence of raw skin oil (1) (SFO 1), raw skin oil (2) (SFO 2), compound dexamethasone acetate cream (DXMS) and ice Huang Fule (BHFL) on pathological tissues of the skin of an affected part of an experimental rat (HE staining result) and a score chart thereof; b is the influence of Bletilla striata extract (Bletilla striata), frankincense extract (frankiness) and Bletilla Striata Polysaccharide (BSP) on pathological tissues of the affected skin of the experimental rat (HE staining result) and a score chart thereof. White line segments in the figure represent the thickened dermal tissue epidermis layer of the rat; white arrows indicate inflammatory cell infiltration; white open arrows indicate subcutaneous fibrous tissue; graphs indicate P values of less than 0.05, 0.01 and 0.001, respectively, compared to the model group. The scales in the figure are 200 micrometers (objective:. Times.4) and 50 micrometers (objective:. Times.20).

FIG. 5 is a graph showing the effect of test drugs on the scrapie frequency of experimental mice. A is the influence of skin oil 1 (SFO 1), skin oil 2 (SFO 2), compound dexamethasone acetate cream (DXMS) and Qingpeng ointment (QP) on the scratching frequency of the experimental mice; b is the influence of Bletilla striata extract (Bletilla striata), frankincense extract (Frankinense) and Bletilla Striata Polysaccharide (BSP) on the scratching frequency of the experimental mice. The "-1" of the horizontal axis identifies the test result before molding; * P <0.05, 0.01 and 0.001, and are each compared to the variability between the test drug group and the model group at the corresponding time points.

FIG. 6 shows the effect of the test drug on the pathological changes of the skin structure at the scratchpad area of the experimental mice. A is the influence of skin oil 1 (SFO 1), skin oil 2 (SFO 2), compound dexamethasone acetate cream (DXMS) and Qingpeng ointment (QP) on the pathological changes of skin structures at the scrapie part of the experimental mouse (HE staining pattern and pathological score pattern at the lower right); b is the effect of Bletilla striata extract (Bletilla striata), boswellia carterii extract (frankinesnse) and Bletilla Striata Polysaccharide (BSP) on the pathological changes of skin structure at the scrapie part (HE staining pattern and pathological scoring pattern at the lower right). White line segments in HE pathology in HE staining plots indicate epidermal thickening; white solid arrows indicate inflammatory cell infiltration; white open arrows indicate subcutaneous fibrous tissue. * P <0.01 and 0.001, and the differences between the test drug groups and the model groups at the corresponding time points, respectively. The scale in the figure is 100 micrometers (objective:. Times.4) and 50 micrometers (objective:. Times.20).

Fig. 7 shows perineal eczema in 81 year old male patients. The left picture shows the state after one month of hospitalization with hormone western medicines (family members refer to the conditions that the color, swelling and itching degree of the affected part are slightly improved compared with the conditions before treatment); the right picture shows the state after the patient is in hospital and the skin oil is externally applied to the part of the patient for ten days (the color and swelling of the affected part can be seen by naked eyes, and the family members refer to the state that the affected part is not scratched by the patient and the symptoms of pruritus can be understood to be relieved).

Fig. 8 is a case of eczema of the right cheek of a female patient aged 40 years. The left picture shows the state of the skin of the first hospital X in Wuhan City (the key special department in Wuhan City, the key special department in combination of Chinese and Western medicine in China, one of four Strength's in the dermatology department in China) after two days (the red and swollen skin of the cheek is serious, a large amount of water bubbles are accompanied, yellow liquid is exuded, and the physical signs such as itching, pain and the like are not basically subsided). The right picture shows that the skin-generating oil is applied for 10 hours (namely, the right cheek is not painful, itchy and red and swollen in the morning the next day, and the broken part is scabbed).

Detailed Description

The applicant shall now make further details of the technical solution of the present invention with reference to specific examples in order to make the present application more clearly understood and appreciated by those skilled in the art.

Example 1: a preparation method of external skin-growing oil for preventing and treating eczema and pruritus comprises the following steps.

Putting 1.62L of sesame oil into a container, then adding 40g of dry powder of each of radix astragali, angelica, gynura procumbens and garden burnet, 20g of dry powder of each of radix angelicae, cynanchum atratum, bletilla striata, frankincense and myrrh, and 10g of dry powder of each of radix scutellariae, rheum officinale, coptis chinensis and golden cypress, and uniformly stirring; and (3) placing the container containing the medicinal materials in a colloid mill, and circularly grinding for 5 hours at normal temperature. Cutting at 20000rpm for 3 times (5 min each time) by flash extraction device; sieving with 5000 mesh sieve under negative pressure, collecting filtered oil, adding 5g Borneolum Syntheticum into the filtered oil, stirring to obtain topical skin caring oil (1), packaging in 100ml sterilized bottles, and sealing for storage.

In addition, for comparison:

preparation of bletilla striata extract: putting 1.62L of sesame oil into a container, adding 20g of bletilla striata dry powder, and uniformly stirring; and circularly grinding for 5 hours in a colloid mill at normal temperature. After stopping, shearing at 20000rpm for 3 times (5 min each time) by flash extraction device; sieving with 5000 mesh sieve under negative pressure, collecting oil, collecting rhizoma bletilla extract, packaging in 100ml sterilizing bottles, and sealing for storage.

Preparation of the frankincense extract: putting 1.62L of sesame oil into a container, adding 20g of dry powder of frankincense, and uniformly stirring; and circularly grinding for 5 hours in a colloid mill at normal temperature. After stopping, shearing at 20000rpm for 3 times (5 min each time) by flash extraction device; sieving with 5000 mesh sieve under negative pressure, collecting oil, collecting external Olibanum extract, subpackaging in 100ml sterilizing bottles, and sealing for storage.

Practice of example 2: a preparation method of external skin-growing oil for preventing and treating eczema and pruritus comprises the following steps.

Putting 1.28L of sesame oil into a container, then adding 30g of dry powder of each of radix astragali, angelica, gynura procumbens and garden burnet, 20g of dry powder of each of radix scutellariae, rheum officinale, coptis chinensis and golden cypress, and 15g of dry powder of each of radix angelicae, cynanchum atratum, bletilla striata, frankincense and myrrh, and uniformly stirring; and (3) placing the container containing the medicinal materials in a colloid mill, and circularly grinding for 2h at normal temperature. After stopping, shearing at 20000rpm for 5 times (5 min each time) by flash extraction device; sieving with 5000 mesh sieve under negative pressure, collecting filtered oil, adding 3g Borneolum Syntheticum into the filtered oil, stirring to obtain topical skin-care oil (2), packaging in 100ml sterilizing bottles, and sealing for storage.

Example 3: a preparation method of rhizoma bletilla polysaccharide for external use for preventing and treating eczema and pruritus comprises the following steps.

Preparation of bletilla striata polysaccharide solution: (1) dissolving dry powder of bletilla striata polysaccharide (purchased from Vickers Biotech, inc., sichuan province, with a purity of 90% or more) in ultrapure water, adding Tween 80 and glycerol to the obtained solution to obtain an aqueous phase containing bletilla striata polysaccharide (wherein the final concentration of Tween 80 is 1.25% V/V; the final concentration of glycerol is 2.81% V/V); (2) the oil phase was prepared by dissolving egg yolk lecithin in sesame seed oil (final concentration of egg yolk lecithin is 6%W/V, g/mL). (3) Under the condition of a constant-temperature water bath at 60 ℃, according to the volume ratio of an oil phase to a water phase of 1:4, dropping the oil phase into the water phase, and stirring at 1500r/min for 10min. (4) Shearing at 20000rpm for 2min, and homogenizing at 1000bar under high pressure for 3 times to obtain bletilla polysaccharide solution with bletilla polysaccharide content of 1mg/ml. Example 4 effects of skin oils (1) and (2) prepared in examples 1 and 2 on DNCB-induced acute eczema model in rats exposed to contact

The test animals were male 6-week-old SPF-grade SD rats 36 (supplied by Liaoning Biotechnology Ltd.). The water diet is freely drunk, the temperature is kept at 25 +/-1 ℃ during the breeding period, and the light and shade period is 12 hours.

1) Group modeling and drug delivery

The 36 rats were randomly divided into 9 groups: blank group, model group, skin oil (1) group, skin oil (2) group, rhizoma Bletillae extract group, olibanum extract group, rhizoma Bletillae polysaccharide group, compound dexamethasone acetate cream group, and compound cortex Phellodendri liquid group.

After 7 days of acclimatization, each rat roughly clipped the hair of the experimental position on the back with a razor close to the skin, exposed the area of the skin position about 3cm x 3cm, and then depilated again with depilatory cream, and immediately washed thoroughly with warm water after depilation to expose the skin of the experimental position for use. After 1 day of depilation, all rats in the remaining groups were subjected to ether inhalation anesthesia, except for the blank group without any injury treatment, placed on a holder, and then sensitized to bare back skin with 100. Mu.L of a 7-percent DNCB acetone solution; after depilation for 2 days, the hair is intensively applied for 1 time in the same way, and sensitization is performed again; after 7 days of dehairing, the rats' back was exteriorized with a pipette with 1% DNCB acetone solution 20. Mu.L challenge. The model is successfully made by the obvious exudation, pimple, erythema and the like in the back area of the rat.

On the day of administration, the wound sites of the model group rats were smeared with 0.25ml of physiological saline except for the blank group; the skin oil (1), skin oil (2), rhizoma Bletillae extract, olibanum extract, and rhizoma Bletillae polysaccharide (prepared in example 3) are applied to wound of rat in each group respectively at an amount of 0.25ml each; the compound dexamethasone acetate cream group is used for smearing 0.25ml of compound dexamethasone acetate cream (Huarun Sanjiu medicine, inc.) on the wound part of each group of rats; the compound cortex Phellodendri liquid composition is prepared by applying 0.25ml of compound cortex Phellodendri liquid (Shandong Hanfang pharmaceutical Co., ltd.) on wound of rats. The administration is 1 time per day and 3 times continuously.

2) Gross observation of skin of tested rats before and after acute eczema modeling

And 4 time periods of the day of successful modeling, 1 day after administration, 2 days after administration, 3 days after administration and the like are selected, and the wound surface on the back of the rat is recorded by using a Sony single-reflex microspur camera to obtain the skin change chart of the rat with the acute eczema in each time period. The severity of the dorsal skin lesions (table 1) of each group of rats was scored against the skin sensitization test scoring criteria table, and in the evaluation process, at least two persons (three persons in this example) were evaluated independently at the same time to avoid subjective factors, and the results were averaged.

Table 1, skin sensitization test score standard table.

3) HE staining and pathological analysis of wounded tissue in test rats

After 3 days of administration, all animals were sacrificed by anesthesia, the skin tissue of the wound site was removed, wrapped with gauze and fixed in formalin for 24 hours. The skin tissue was then sequentially subjected to running water washing, dehydration, paraffin embedding, followed by tissue sectioning, HE routine staining, and observation and photography under an optical microscope.

Pathological scoring of rat skin lesion tissue: rat skin tissues were pathologically scored under light microscopy according to the following inflammatory scoring table (table 2) and expressed as mean ± SEM.

TABLE 2 pathological scoring of rat skin lesion tissue.

4) Results of the experiment

(1) Gross observation of skin in DNCB-induced acute eczema rats

As shown in FIG. 1, the skin of the rats in the blank group was observed to be reddish, fine, tender, soft and clear in texture during the period from the day when the acute eczema induced by DNCB was successfully modeled to 3 days after the administration.

a) After the model group rats are successfully modeled, severe erythema and severe edema occur on the wound surface on the same day, accompanied by exudation and eschar formation: after 1 day of administration, the wound skin eschar area of the rats in the model group becomes small, large scale remains, and the degree of erythema and edema is slightly reduced; after 2 days of administration, the skin wound of the rats in the model group is further reduced, eschar still exists, the color of the eschar is darkened, but the degree of erythema and edema is reduced after 1 day; after 3 days of administration, the wound still has a small part of crust skin, and is accompanied by more obvious erythema and edema.

b) Compared with the model group, the skin erythema, edema and eschar of other groups of rats are improved, particularly, the skin oil (1) group of the rats is administrated for 1 day, the skin eschar area of the rats is rapidly reduced, the color of the eschar is lighter, and the erythema and edema area is obviously reduced; after 2 days of administration, skin oil (1) produced in rats has no erythema on skin around scab surface; after 3 days of administration, the skin crusts and scales of the rats in the raw skin oil (1) group are peeled off.

c) Compared with the model group, the skin oil (2) group also has good treatment effect: after 1 day of administration, the area of the rat skin crust skin is rapidly reduced, the color of the crust skin is lighter, and the degree of erythema and edema is slightly reduced; after 2 days of administration, the skin scab surface of the rats in the raw skin oil (2) group became small; after 3 days of administration, the skin crusts and scales of the rats in the raw skin oil (2) group mostly fall off, only a small part remains, and small-area erythema exists.

d) Compared with the treatment effect of the skin oil (1) group, the compound dexamethasone acetate cream group and the compound phellodendron bark liquid group which simulate clinical medication are effective, but the effect of the compound dexamethasone acetate cream group and the compound phellodendron bark liquid group is still not slightly different from that of the skin oil (1) group:

the compound dexamethasone acetate cream group still has large area of eschar on the skin of the wound of the rat after 1 day of administration, and the color of the eschar is darker than that of the skin oil (1) group; a small eschar slough off with erythema edema 2 days after administration; the crust skin did not completely peel off after 3 days of administration and was partially red.

The compound cortex Phellodendri liquid group rat skin still has large area eschar after administration for 1 day; a small eschar was left 2 days after dosing with erythema edema; a small portion of crust remained 3 days after administration with red swelling.

As shown in fig. 1B, bletilla striata extract, boswellia extract and bletilla striata polysaccharide, which are components of the skin-generating oil and active monomers thereof, also showed a certain therapeutic effect.

e) The skin of the rat in the bletilla striata extract group is scabbed and the color of the scab skin is darkened after 1 day of administration; the crust skin part falls off after 2 days of administration, and edema and erythema still exist around the crust surface; a small portion of crust skin, erythema remained 3 days after administration.

f) The large area of crust skin still remained after 1 day of administration for the rats in the frankincense extract group; a small eschar was left 2 days after administration with edema; the crust remained present and edema was present 3 days after administration.

g) Compared with the recovery effect of the bletilla striata extract group and the frankincense extract group, the bletilla striata polysaccharide group prepared in example 3 performs better: the color of the crust became darker and the area of the eschar became smaller after day 1 of administration; most of eschar and scales fall off after the administration day 2, and erythema and edema are remained; erythema and edema disappeared after day 3 of administration, leaving small-area scales and crusts without shedding.

During the period from the successful model building to 3 days after the administration, the severity of the back skin lesions of the rats in each group is scored according to the skin sensitization test scoring standard table (the full score is 14; the scoring table is shown in table 1), the score of the rats in the blank group is 0, and the rats with the acute eczema can be found according to the appearance scoring chart (figure 1):

(1) The overall score change trend of the model group rats is slow from the day of successful model building to the day after 3 days of drug administration;

(2) Compared with the model group, the score change of each tested drug group is larger:

at 1 day after administration, the rat skin appearance scores of both the skin oil (1) group (10.0 ± 0.4 p-straw 0.05) and the skin oil (2) group (11.0 ± 0.4) were smaller compared to the model group (12.5 ± 0.3 p-straw 0.001), indicating that both skin oils (1), (2) were reparative; compared with the compound phellodendron amurense ointment group (11.3 +/-0.5) and the compound phellodendron amurense liquid group (12.3 +/-0.3), the compound dexamethasone acetate cream group has no obvious difference between the groups.

At 2 days after dosing, the appearance scores were significantly lower for the crude oil (1) group (3.2 ± 0.4 p-cloth 0.001) and the crude oil (2) group (4.3 ± 0.3 p-cloth 0.001) compared to the model group (10.0 ± 0.4;

at 3 days after dosing, the skin of the rats in the skin oil (1) group had tended to be completely repaired compared to the model group (8.5 ± 0.3) (1.5 ± 0.3 p-straw 0.001; the skin wound of rats in the skin oil (2) generation group is slightly red and swollen, but is close to recovery (2.0 +/-0.4P-cloth-type 0.001); the skin of rats in the compound dexamethasone acetate cream group (4.0 plus or minus 0.4P-woven fabric (P-woven fabric) 0.01) and the compound cortex phellodendri liquid group (3.5 plus or minus 0.5P-woven fabric (P-woven fabric) still has crust and red swelling.

In contrast, the data show that the skin-generating oil (1) and the skin-generating oil (2) are superior to the compound dexamethasone acetate cream and the compound phellodendron bark liquid in the repair capability and treatment effect on the skin.

In addition, the applicant also studied the therapeutic effects of the bletilla striata extract group, the frankincense extract group and the bletilla striata polysaccharide group (prepared in example 3) of the active monomers of the raw skin oil (fig. 1B), and found that the treatment effects are less than those of the raw skin oil (1) and the raw skin oil (2), but the treatment effects show a certain repairing trend compared with the model group.

At 1 day after administration, the rat skin appearance scores of the bletilla striata extract group (10.7 ± 0.2 p-straw 0.05), the frankincense extract group (11.0 ± 0.5), the bletilla striata polysaccharide group (10.2 ± 0.3 p-straw 0.01) were all smaller compared to the rat appearance score of the model group (12.5 ± 0.3);

at 2 days after administration, the rat skin appearance scores of the bletilla striata extract group (6.0 ± 0.4 p-cloth-0.01), the frankincense extract group (7.0 ± 0.4 p-cloth-0.05), the bletilla striata polysaccharide group (5.7 ± 0.2 p-cloth-0.01) were closer compared to the rat appearance score of the model group (10.0 ± 0.4);

at 3 days after administration, the bletilla striata extract group (3.3 ± 0.5 p- < -0.01) and bletilla striata polysaccharide group (2.8 ± 0.4 p < -0.001) scored closer by comparison compared to the rat appearance score of the model group (8.5 ± 0.3), whereas the frankincense extract group (4.3 ± 0.5 p < -0.01) was slightly less effective than the bletilla striata extract group and bletilla striata polysaccharide group.

(2) HE staining observation and pathological analysis of DNCB-induced acute eczema rat wound tissue

After 3 days of administration (day 10 of the experiment), all the rats were sacrificed by anesthesia, and the skin tissue of the wound site was taken and HE-stained, and the results of pathological analysis were shown in fig. 2.

The cells in the epidermal layer and the dermal layer of the blank group of rats are normally arranged without edema, and a large number of hair follicles and other accessory organs are visible without abnormal pathological morphological changes.

a) Compared with the blank control group, the skin tissue of the rats in the model group presents: (1) the epidermis layer structure is incomplete, the proportion of the epidermis layer and the dermis layer is disordered, the stratum corneum and the granular layer are thickened, the spinous layer is thickened, and the epidermis protrusion is widened and prolonged; (2) the dermis layer can be seen with a large amount of sweat gland hyperplasia and inflammatory cell infiltration; (3) the pathological score of skin tissues (2.7 ± 0.5 p <0.001) was significantly less than that of the blank group (10.0 ± 0.0).

b) Compared with the model group, the skin oil (1) group shows the skin tissue of the acute eczema rat: (1) the epidermal layer has a complete structure, the cuticle is thinner than a model group, the epidermal protrusion is not widened and prolonged, and mild parakeratosis is caused; (2) the dermis layer can be seen to grow hair of skin appendages such as hair follicles; (3) the skin histopathological scores (7.8 +/-0.4P & lt 0.001) were significantly higher than those of the model group.

c) Compared with the model group, the skin oil (2) group shows the skin tissue of the acute eczema rat: (1) the epidermal layer has a complete structure, and the skin cuticle is obviously thinner than a model group; (2) the dermis layer can see a large number of hair follicles and other skin appendages for hair growth; (3) the skin histopathology scores (7.5 ± 0.6 p <0.001) were significantly higher than in the model group.

d) However, compared with the model group, the compound dexamethasone acetate cream group has a less obvious curative effect: (1) the epidermal layer structure is disordered and rough, the epidermis is hyperkeratotic, the thickening of the horny layer and the granular layer is not obviously improved compared with a model group, and the width of the epidermal protrusion is increased and prolonged; (2) the dermis layer can be seen to grow hair of skin appendages such as hair follicles and is accompanied by a large amount of inflammatory cell infiltration and red blood cell overflow; (3) the skin histopathology score (4.7 + -0.5) was slightly higher than that of the model group.

e) Meanwhile, compared with the model group, the skin oil (1) group and the skin oil (2) group, the treatment effect of the compound phellodendron amurense liquid group is not ideal: (1) although the epidermal layer has a complete structure, the cuticle layer is thickened, the acanthosis is not obviously improved, and the width of the epitome is increased and prolonged; (2) the dermis layer can be seen in the condition that hair follicles and other skin appendages grow hair and are accompanied by inflammatory cell infiltration and red blood cell overflow; (3) the pathological score of skin tissues (6.0 +/-0.4P < -0.01) is higher than that of a model group.

Bletilla striata, frankincense and bletilla striata polysaccharides as components of skin oil and their active monomers also showed some effect compared to the model group (fig. 2B).

f) Bletilla striata extract group rat skin tissue: (1) the whole layer structure of the skin is complete, the epidermis can be obviously cornified, and the stratum corneum and the granular layer are thickened; (2) the dermis layer can be seen with hair follicle and other skin accessory hair growth accompanied by moderate inflammatory cell infiltration and congestion and edema among tissues; (3) the pathological score of skin tissues (6.0 +/-0.4P < -0.001) is higher than that of the model group.

g) The skin tissue condition of the frankincense extract group rats is poor: (1) the whole layer structure of the skin is complete, the epidermis layer can be obviously cornified, the stratum corneum and the granular layer are thickened, the spinous layer is thickened, and the width of the epidermal process is increased and prolonged; (2) the dermis layer is seen with massive sweat gland hyperplasia with moderate inflammatory cell infiltration and red blood cell extravasation; (3) the skin histopathology scores (5.2 ± 0.5 p <0.01) were slightly higher than in the model group.

h) The skin tissues of the bletilla striata polysaccharide group have obvious effects in three groups: (1) the whole layer structure of the skin is complete, the stratum corneum of the skin is thinner than that of a model group, and the epidermis process is slightly widened and prolonged; (2) the dermis layer can see a large number of hair follicles and other skin appendages for growing hair, and obvious inflammatory cell infiltration and red blood cell overflow are not seen; (3) the skin histopathology scores (6.5 ± 0.6 p <0.001) were significantly higher than in the model group.

Example 5 Effect of skin oils (1) and (2) prepared in examples 1 and 2 on DNCB-induced model of chronic eczema contactable in rats

The test animals were male 6-week-old SPF-grade SD rats 36 (supplied by Liaoning Biotechnology Ltd.). The water diet was freely drunk, the temperature was kept at 25 + -1 deg.C during the feeding period, and the light and dark cycle was 12 hours.

1) Group modeling and drug delivery

The 36 rats were randomly divided into 9 groups: blank group, model group, skin oil (1) group, skin oil (2) group, rhizoma Bletillae extract group, olibanum extract group, rhizoma Bletillae polysaccharide group, compound dexamethasone acetate cream group, and ice Huang Fule ointment group.

After adaptive feeding for 7 days, each rat was roughly cut off the hair of the rat at the experimental position on the back by sticking the skin to the razor, the area of the exposed skin position was about 3cm × 3cm, and then depilated again with depilatory cream, and immediately after depilating, the rat was thoroughly washed with warm water to expose the skin of the experimental position for use. On day 1 after depilation, all rats in the remaining groups were subjected to ether inhalation anesthesia except for the blank group without any injury treatment, placed on a holder, and then sensitized to bare back skin with 100. Mu.L of a 7% DNCB acetone solution; after 1 week, the sheared area of the back of the rat was dehaired again, and the next day was overcoated with 5% of DNCB acetone solution (50. Mu.L), to carry out sensitization 2. Sensitization was continued thereafter 1 time every 5 days for 3 consecutive times. The appearance of the skin of the back area of the rat is changed into the appearance of the skin of the rat, such as thickened skin, rough surface, pimple, red swelling and the like, so that the model is successfully made.

On the 3 rd day after the model building experiment is successful, 0.25ml of normal saline is smeared on the wound part of the rats in the model group except the blank group; the skin oil (1), skin oil (2), bletilla striata extract, frankincense extract and bletilla striata polysaccharide (1 mg/ml; bletilla striata polysaccharide solution prepared in example 3) are respectively applied to the wound of rats of each group with 0.25ml of each of the skin oil (1), the skin oil (2), the bletilla striata extract, the frankincense extract and the bletilla striata polysaccharide; the compound dexamethasone acetate cream group is used for smearing 0.25ml of compound dexamethasone acetate cream on the wound part of a rat; ice Huang Fule ointment group the wounded part of the rat was coated with 0.25ml of ice Huang Fule ointment (Tibet sea Rong Tang fruit pharmaceutical Co., ltd.). The administration is performed 1 time per day and 3 times continuously.

2) Gross observation of skin of tested rats before and after chronic eczema modeling

And (3) selecting 3 time periods of the successful modeling day, 1 day after administration, 3 days after administration and the like, and recording the wound surface on the back of the rat by using a Sony single-reflex microspur camera to obtain a skin change chart of the chronic eczema rat in each time period. The severity of the back skin lesions of each group of rats was scored against a skin sensitization test scoring criteria table (table 1), and in the evaluation process, two persons were required to perform independent evaluation at the same time to avoid subjective factors, and the results were averaged.

3) HE staining and pathological analysis of wounded tissue in test rats

After 3 days of administration, all animals were sacrificed by taking a picture and then anaesthetizing, the skin tissue of the wound site was removed, wrapped with gauze and fixed in formalin for 24 hours. The skin tissue was then sequentially subjected to running water rinsing, dehydration, paraffin embedding, followed by tissue sectioning, HE conventional staining, and observation and photographing under an optical microscope.

Pathological scoring of rat skin lesion tissue: rat skin tissues were pathologically scored under light microscopy according to the inflammatory scoring table (table 2) and expressed as mean ± SEM.

4) Results of the experiment

(1) Gross observations of DNCB-induced chronic eczema in rat skin

a) As shown in FIG. 3, in the period from the successful modeling of the DNCB-induced chronic eczema to 3 days after the administration, the skin of the rats in the blank group is observed to be light red, fine, tender, soft and clear in texture.

b) After the model group rats are successfully modeled, the wound surface has severe erythema and edema accompanied with severe denudation and scale: after 1 day of administration, the scale and stripping area of the wound surface skin of the rat in the model group become small, and the degree of erythema and edema is slightly reduced; after 3 days of administration, the skin wound of the rats in the model group is further reduced, scales and exfoliation still exist, and erythema and edema still exist.

c) Compared with the model group, the skin erythema, edema, scale and exfoliation of other rats in each group are improved, particularly, the scale and exfoliation area of the skin of the rat in the skin oil (1) group is rapidly reduced after 1 day of administration, the color of crust is lighter, and the areas of erythema and edema are obviously reduced; after 3 days of administration, the skin of the rats in the skin-generating oil (1) group had no edema and erythema, scales were shed, the wound surface was almost invisible, and hair was grown.

d) Meanwhile, compared with the model group, the skin oil (2) group also has good treatment effect: after 1 day of administration, the rat skin rapidly scabs and removes the scab, and the color of the scab skin is lighter, and the areas of erythema and edema are obviously reduced; after 3 days of administration, the skin crusts and scales of the rats in the raw skin oil (2) group completely shed, and only small-area erythema exists.

e) Compared with the treatment effects of the raw skin oil (1) and the raw skin oil (2), the compound dexamethasone acetate cream group and the ice Huang Fule ointment group which simulate clinical medication are effective compared with the model group, but the effects of the dexamethasone acetate cream group and the ice Huang Fule ointment group are still not slightly different from the effects of the raw skin oil group:

the compound dexamethasone acetate cream group still has large scale on the skin of the wound of the rat after 1 day of administration, and the color of the crust skin is deeper than that of the skin-generating oil (1) and (2) groups; a small part of the crust skin still does not fall off after 3 days of administration, and erythema edema is accompanied.

The skin of the rats with the Binghuangfule ointment group has most scales and darker crust skin after 1 day of administration; the crust skin remained incompletely detached after 3 days of administration, with erythema edema.

In the research of the present application, bletilla striata extract, boswellia extract and bletilla striata polysaccharide, which are components of the skin-generating oil and active monomers thereof, also showed certain effects.

f) The skin of the rat with the bletilla striata extract group is scabbed after 1 day of administration, the color of the scab skin is dark, and erythema and edema are formed around the scab surface; the crust skin part fell off after the 3 rd day of administration, leaving a small part of crust skin, and the crust skin was accompanied by erythema and edema.

g) The frankincense extract group rats still have large scale, erythema and edema after being administrated for 1 day; the crust remained present and edema was present 3 days after administration.

h) Compared with the recovery effect of the bletilla striata extract group and the frankincense extract group, the bletilla striata polysaccharide (1 mg/ml; bletilla striata polysaccharide solution prepared in example 3) group performed better: the area of the crust surface becomes smaller after 1 day of administration; after 3 days of administration, most of the scales fall off, leaving a small crust, with small-area erythema.

During the period from the success of the experimental modeling to 3 days after the administration, the severity of the back skin lesions of each group of rats was scored according to the skin sensitization test scoring standard table (full score was 12 points), and the score of the blank group of rats was 0 point.

a) According to the appearance score chart (figure 3) of the chronic eczema rats, the overall score of the model group rats slowly changes from the day of model building success to 3 days after administration;

b) While skin oil score changes significantly:

after 1 day of administration, the skin appearance scores of rats in the raw skin oil (1) group (5.0 ± 0.4P-straw 0.01) were smaller than those in the model group (10.0 ± 0.4P-straw 0.001), and were also smaller than those in the raw skin oil (2) group (6.0 ± 0.4P-straw 0.01), indicating that the skin repair abilities of the raw skin oil (1) and the raw skin oil (2) were good; whereas the compound dexamethasone acetate cream group (8.8 ± 0.5) and the ice Huang Fule ointment group (6.8 ± 0.5 p-woven-fabric-0.05) showed less change in skin appearance score of rats compared to the model group.

At 3 days after dosing, the rat skin in the raw oil (1) group had tended to be completely repaired (2.0 ± 0.4 p-leguminous 0.001; skin oil (2) group rats had a slight red and swollen skin wound but were also nearly recovered (2.7 ± 0.2 p-woven fabric 0.05).

c) The skin of rats in the compound dexamethasone acetate cream group (4.0 +/-0.4) and the ice Huang Fule ointment group (3.5 +/-0.6) still has crust and red swelling. The comprehensive data show that the skin-growing oil, whether the skin-growing oil (1) or the skin-growing oil (2) has better skin repairing capability and treatment effect than the compound dexamethasone acetate cream and the ice Huang Fule ointment.

d) Compared with the model group, the bletilla striata extract group, the frankincense extract group and the bletilla striata polysaccharide group also show a certain repairing trend, but the treatment effect is not as good as that of the skin-generating oil group:

at 1 day after administration, the rat skin appearance scores of the bletilla striata extract group (6.8 ± 0.3 p-straw 0.001), the frankincense extract group (7.3 ± 0.7 p-straw 0.01), the bletilla striata polysaccharide group (6.0 ± 0.4 p-straw 0.01) were all smaller compared to the model group rat appearance score (10.3 ± 0.3);

3 days after administration, the appearance score of the model group rats is 7.3 +/-0.4; by comparison between groups, the bletilla striata polysaccharide group scored lowest (3.3 ± 0.3 p-straw 0.01), the frankincense extract group (4.5 ± 0.7) was slightly less effective than the bletilla striata extract group (3.8 ± 0.3 p-straw 0.01) and bletilla striata polysaccharide group.

2) HE staining observation and pathological analysis of DNCB-induced chronic eczema rat wound tissue

After 3 days of administration, all the rats were sacrificed by anesthesia, and the results of pathological analysis are shown in FIG. 4 after HE staining of the skin tissue of the wound site. It can be seen that the blank group of rats had normal arrangement of epidermal and dermal cells, no edema, and a large number of hair follicles and other accessory organs are visible without any abnormal pathological morphological changes.

a) Compared with the blank control group, the skin tissue of the rats in the model group presents: (1) the epidermal layer structure is fuzzy, the proportion of the epidermal layer and the dermal layer is unbalanced, obvious keratinization is seen, the stratum corneum and the granular layer are thickened, the spinous layer is thickened, and the width of the epidermal protrusion is increased; (2) dermal inflammatory cell infiltration; (3) the pathological score of skin tissues (1.5 ± 0.3 p <0.001) was significantly less than that of the blank group (10.0 ± 0.0).

b) Compared with the model group, the skin oil (1) group (8.3 +/-0.4; p < 0.001) the skin tissue of rats with chronic eczema behaves close to normal skin tissue: (1) the epidermal layer structure is clear and complete, the epidermal layer of the skin is obviously thinner than the model group, and the skin is slightly keratinized; (2) the dermis layer can show hair follicles and other accessory growth accompanied by a small amount of inflammatory cell infiltration; (3) the skin histopathology score was significantly higher than the model group.

c) Compared with the model group, the skin oil (2) group (7.3 ± 0.4; p < 0.001) the skin tissue of rats with chronic eczema behaves close to normal skin tissue: (1) the epidermal layer structure is clear and complete, and the epidermal layer of the skin is thinner than the model group and slightly keratotic; (2) the dermis layer can show the growth of attachments such as hair follicles and the like; (3) the skin histopathology score was significantly higher than the model group.

d) However, compared to the model group, the compound dexamethasone acetate cream group (3.8 ± 0.5; p < 0.05) the efficacy is not very significant: (1) the epidermal layer structure is fuzzy, hyperkeratosis is caused, the thickening of the horny layer and the granular layer is not obviously improved compared with a model group, and the spinous layer is thick; (2) thickening of the dermal layer, visible fibrous hyperproliferation with moderate inflammatory cell infiltration; (3) the skin histopathology score was slightly higher than the model group.

e) Meanwhile, compared with the model group and the skin oil (1) and (2) groups, the ice Huang Fule ointment group (5.2 ± 0.4; p < 0.01) is also less than ideal: (1) compared with a model group, the thickening of the epidermal layer is not obviously improved, hyperkeratosis, cell gap thickening, acanthosis thickening is not obviously reduced, and the width of the epidermal process is increased and prolonged; (2) thickening of the dermis with visible fibrous hyperproliferation with high inflammatory cell infiltration and erythrocyte extravasation; (3) the pathological score of the skin tissue is higher than that of the model group.

Compared with the model group, the bletilla striata extract group, the frankincense extract group and the bletilla striata polysaccharide group also show a certain repairing curative effect:

f) Bletilla striata extract group rat skin tissue: (1) the structure of the epidermal layer is not clear, the epidermal layer is thickened, and the intercellular space is thickened; (2) inflammatory cell infiltration, interstitial hyperemia and edema in the dermis layer; (3) the pathological score of skin tissues (5.0 +/-0.4P < -0.001) is higher than that of the model group.

g) The skin tissue condition of the frankincense extract group rats is poor: (1) hyperkeratosis of epidermis appears, stratum corneum and granular layer are obviously thickened, acanthosis is thickened, and the width of the epitome is increased and prolonged; (2) more inflammatory cell infiltration and extravasated red blood cells are visible in the dermis; (3) its skin histopathological score (3.5 ± 0.3 p < -0.01) was slightly higher than that of the model group.

h) The skin tissue of the bletilla striata polysaccharide group performed better in three groups: (1) the epidermis is clear and complete, the skin cuticle is thinner than the model group, and the keratinized substances are slightly thickened; (2) the dermis layer is infiltrated by a small amount of neutrophils; (3) the skin histopathology scores (6.7 ± 0.2 p <0.001) were significantly higher than in the model group.

EXAMPLE 6 Effect of skin oils (1) and (2) prepared in examples 1 and 2 on the Combined use of Histamine and 4-aminopyridine in the pruritus model

The test animals were male 6-week-old SPF-grade KM mice 45 (provided by longshongning biotechnology, inc.). The water diet was freely drunk, the temperature was kept at 25 + -1 deg.C during the feeding period, and the light and dark cycle was 12 hours.

1) Group modeling and drug delivery

Healthy SPF-grade KM mice, 45, were randomly divided into 9 groups: blank Control group (Control), model group (Model), raw skin oil (1) group (SFO 1), raw skin oil (2) group (SFO 2), bletilla striata extract group (Bletilla striata), frankincense extract group (Frankincense), bletilla striata polysaccharide group (BSP), compound dexamethasone acetate cream group (DXMS) and QINGZHENGQIPENG ointment group (QP). After adaptive feeding for 7 days, roughly cutting off the hair behind the ears of the neck and the back of each mouse by tightly adhering a shaver to the skin, exposing a circular area with the diameter of about 1.0cm at the position of the skin, and removing hair again by using hair removal cream; after depilation, thoroughly washing with warm water immediately to expose the skin of the experimental part for later use; after 1 day of depilation, recording the itching frequency of each group of mice before administration by video; after 2 days of depilation, all mice in the remaining groups were injected subcutaneously with histamine (2 mg/ml, 50. Mu.l) and 4-aminopyridine (0.8 mg/ml, 50. Mu.l) at the back of the neck for inducing scrapie behavior in the mice, except for the control group, which was not injected subcutaneously.

In the modeling process, except for a blank control group, animals in a model group, a skin oil (1) group, a skin oil (2) group, a bletilla striata extract group, a frankincense extract group, a bletilla striata polysaccharide group, a compound dexamethasone acetate emulsifiable paste group and a Qizhengqingpeng ointment group are respectively injected with histamine (2 mg/ml and 50 mu l) and 4-aminopyridine (0.8 mg/ml and 50 mu l) subcutaneously, immediately placed in a transparent observation box for observation for 30min, and a digital camera is used for recording the scratchiness incubation period and the total scratchiness times. In the second injection, except for the control group and the model group, the shaved part of each mouse in the other groups was coated with 0.25ml each of the skin oil (1), the skin oil (2), the bletilla striata extract, the frankincense extract, the bletilla striata polysaccharide (1 mg/ml; the solution of the bletilla striata polysaccharide prepared in example 3), the compound dexamethasone acetate cream (Huarun Sanjiu medicine, inc.) and the Qizhengqingpeng ointment (Xizang Qizheng medicine, inc.) 1 time a day for 4 days until the end of the experiment.

2) Behavioral observation of scratching and itching scoring in test mice

The severity of itching between groups was observed by the number of behavioral tickles of the mice. After injecting histamine and 4-aminopyridine subcutaneously at the back of the neck of the mouse, the mouse is immediately placed in a transparent observation cage, and is photographed for 30min in an unmanned quiet environment, and the times of scratching behaviors of the mouse are recorded. The shaved area was scraped with the mouse by lifting the hind paw and the paw was returned to the floor or mouth until it stopped being scored as 1 scratchback (successive scratchings were also scored as 1). The itch frequency was recorded for each group of mice (fig. 5).

3) Wound surface tissue of test mouse HE staining and pathological analysis of

After 4 days of administration (i.e., the day of completion of the experiment), all the animals were sacrificed by anesthesia, and the skin tissue at the wound site was removed, wrapped with gauze, and fixed in formalin for 24 hours. The skin tissues were then sequentially subjected to running water washing, dehydration, paraffin embedding, followed by tissue sectioning, HE routine staining, and observation under an optical microscope, and the skin tissues were pathologically scored (fig. 6).

4) Results of the experiment

(1) Case analysis of pruritic mice

The number of 30min scrapings in the cages was observed transparently for mice with pruritus caused by the combination of histamine and 4-aminopyridine at 6 time periods (1 day before, only, 1 day after, 2 days after, 3 days after, 4 days after the administration of the model drug), recorded in the planned administration (the results are shown in fig. 5).

a) Fig. 5A shows that the molding scheme of the experiment is very successful, the scratchback frequency of each group of mice is obviously improved after the mice are dosed with the molding agent on the molding day, and the scratchback frequency is generally within the range of 30-40 times within 30 min. The number of scratchings of the blank groups did not change significantly after 1, 2, 3 and 4 days after administration (1.2 + -0.3, 1.4 + -0.2, 2.0 + -0.5 and 1.6 + -0.4, respectively);

b) The number of scratchings significantly increased in model mice after 1, 2, 3, 4 days post-dose compared to the blank group (37.8 ± 1.8, respectively, p-herds 0.001;36.6 +/-1.8, and P is woven into a layer of cloth with the length of 0.001;35.0 +/-1.3, and P (P) woven fabric layer is less than 0.001;34.2 + -1.5, P-woven fabric of 0.001). The itch frequency of the blank and model mice remained relatively constant during the experiment, indicating that the molding protocol was successful.

c) Skin oil generation after 1, 2, 3, 4 days after administration (1) mice were scratchy times (23.0 ± 1.8, respectively, p-cloth 0.001;12.6 +/-3.0, and P (P) woven fabric layer is less than 0.001;14.0 +/-1.3, and P (P) woven fabric layers are all covered with 0.001;9.6 plus or minus 1.0; p < 0.001) showed a significant decrease with time of administration with significant differences. It can be seen that the skin oil (1) has an excellent therapeutic effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

e) Compared with the model group, the numbers of scratchings of the mice in the skin oil (2) group after 1, 2, 3 and 4 days after administration (33.5 ± 1.0;15.2 +/-1.0, and P (P) woven fabric is less than 0.001;10.2 +/-2.3, and P (P) woven fabric layer is less than 0.001;13.5 ± 0.5, p-straw-woven 0.001) also exhibited a significant decrease with time and was significantly different after 2, 3, 4 days post-administration. The results show that the skin-growth oil (2) has a very good therapeutic effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

f) Compared with the model group, the mice of the compound dexamethasone acetate cream group had

scratchings

1, 2, 3, 4 days after administration (28.6 ± 3.1, respectively, p-s-0.01; 16.0 +/-0.7, and P is woven into a layer of cloth with the length of cloth being less than 0.001;15 +/-1.3, P is covered with 0.001;16.4 ± 2.3, p-s-woven 0.001) also showed significant decrease with time of administration, with significant differences. The results show that the compound dexamethasone acetate cream also has good treatment effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

g) Compared with the model group, the number of scratchings of mice in the qizhengpeng ointment group (25.8 ± 0.9, respectively, p-herd 0.001;22.8 +/-1.4, and P is covered with 0.001;21.0 +/-2.2, and P is woven into 0.001;17.3 ± 2.0, p-Ap-0.001) also showed a significant decrease with the lapse of the administration time, with significant difference, suggesting that the Qingpeng ointment also showed a better therapeutic effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

Compared with the improvement effect of four medicines on the scratching frequency, the Qingpeng ointment, the compound dexamethasone acetate cream, the skin-generating oil (1) and the skin-generating oil (2) have good treatment effects, wherein the skin-generating oil (1) has the best effect.

As can be seen from fig. 5B, the scrapie frequency of mice in the bletilla striata polysaccharide (bletilla striata polysaccharide solution prepared in example 3), bletilla striata extract and boswellia extract groups all showed a certain degree of decrease with the time of administration.

h) Compared with the model group, after 1, 2, 3 and 4 days after administration, the numbers of scratchings of the mice in the bletilla striata polysaccharide group (37.4 +/-1.9; 20.4 +/-1.5, and P (P) woven fabric is less than 0.001;23.8 +/-3.9, and P is covered with 0.01;22.2 ± 1.3, p-s-cloth of 0.001) also exhibited a significant decrease over time with significant differences after 2, 3, 4 days post-administration. The result shows that the bletilla striata polysaccharide has a certain relieving effect on pruritus caused by combined use of histamine and 4-aminopyridine, but the effect is not obvious.

i) Compared with the model group, after 1, 2, 3 and 4 days after administration, the numbers of scratchings of the mice in the bletilla striata extract group (respectively 30.8 +/-1.9; 28.6 plus or minus 1.8;22.2 +/-2.4, and P is woven into 0.001;20.8 ± 1.8, p-s-tres of 0.001) also showed a significant decrease with time of administration, with significant differences after 3 and 4 days post-administration. The results show that the bletilla striata extract has certain treatment effect on pruritus caused by combined use of histamine and 4-aminopyridine.

j) Compared with the model group, the scratchy times of the frankincense extract group mice after 1, 2, 3 and 4 days after administration are respectively (19.2 +/-0.8; 18.6 +/-1.6, P (P) is covered with 0.001;22.6 +/-3.9, and P is covered with 0.001;21.4 ± 2.0, p-woven fabric of 0.001) also showed a significant decrease with time of administration with significant differences; the frankincense extract also has a certain treatment effect on pruritus caused by the combined administration of histamine and 4-aminopyridine.

Comparing the above three medicines for relieving pruritus frequency, the Olibanum extract has the best effect.

(2) Results of histopathology experiments

The pathological analysis results of all the mice subjected to cervical vertebra removal and died (4 days after modeling) after HE staining of the affected skin are shown in fig. 6.

a) As can be seen from FIG. 6A, the epidermal layer of the mice in the blank group was structurally intact, and the epithelial cells were arranged in a polar manner; a large number of hair follicles and other accessory organs can be seen subcutaneously, and abnormal pathological morphological changes such as edema and inflammatory cell nest infiltration are not seen.

b) Compared with a blank control group, the skin of the scrapie part of the model group of mice: (1) the epidermis layer is partially deleted and structurally disordered, and the epidermis layer is obviously thickened; (2) obvious edema and a large amount of inflammatory cell nest infiltration can be seen in the dermis layer; (3) the pathological score of skin tissues (2.0 + -0.3, P < <0.001) is significantly less than that of blank group (9.0 + -0.3).

c) Compared with the model group, the skin oil (1) group mice have skin appearance at the scrapie part: (1) the epidermal layer has a complete structure, epithelial cells are arranged in a polar manner, and obvious thickening is not seen; (2) follicular hyperplasia and a small amount of inflammatory cell infiltration are seen in the dermis; (3) the skin histopathology score (7.6 ± 0.2 p <0.001) was significantly higher than in the model group.

d) Compared with the model group, the skin of the scrapie part of the mice in the skin oil (2) generation group is presented: (1) the epidermal layer has a complete structure, the thickness of the epidermal layer is equivalent to that of the blank group, and epithelial cells are arranged in a polar manner; (2) the dermis layer can be seen with a large amount of hair follicle hyperplasia and a small amount of inflammatory cell infiltration; (3) the skin histopathology score (7.0 ± 0.5 p <0.001) was significantly higher than in the model group.

e) Compared with the model group, the compound dexamethasone acetate cream group shows the skin at the scrapie part of the mouse: (1) the epidermal layer has a complete structure and is slightly thickened; (2) follicular hyperplasia and mild edema and a small amount of inflammatory cell infiltration are seen in the dermis; (3) the skin histopathology score (6.8 ± 0.3 p <0.001) was significantly higher than in the model group.

f) Compared with the model group, the skin of the scrapie part of the Qingpeng ointment group mice is presented: (1) the epidermal layer has a complete structure and is slightly thickened; (2) the dermis layer can be seen with little hair follicle hyperplasia and little inflammatory cell infiltration; (3) the skin histopathology score (5.4 ± 0.2 p <0.001) was significantly higher than in the model group.

g) As can be seen from fig. 6B, in the model group control, the skin of the scrapie site of the mice in the boswellia extract group appears: (1) the epidermal layer has complete structure and slightly thickened, and epithelial cells can be arranged; (2) edema and local inflammatory cell infiltration in the dermis layer; (3) the skin histopathology score (5.6 +/-0.2, P < -0.001) was significantly higher than that of the model group.

h) Compared with the model group, the skin of the mouse scrapie part of the bletilla striata extract group is presented: (1) the epidermal layer has complete structure and slightly thickened, and epithelial cells can be arranged; (2) the dermis layer can be seen with hyperplasia of skin appendages such as hair follicles; (3) the skin histopathological score (4.6 +/-0.2, P < -0.001) was higher than that of the model group.

i) Compared to the model group, the skin appearance of the scrapie part of the mice in the bletilla striata polysaccharide (bletilla striata polysaccharide solution prepared in example 3) group: (1) partial loss of epidermal layer structure, (2) proliferation of skin appendages such as hair follicles and infiltration of local inflammatory cells in the dermis layer; (3) the skin histopathological score (3.4 +/-0.2; P < -0.01) is higher than that of the model group. Example 7 skin oil preparation prepared in example 1 (i.e., topical skin oil (1)) representative case one (1) treatment of perineal eczema

Mr. 81 years old has incomplete urination, and residual urine easily leaks into underpants, so perineal eczema is caused to be nearly more than ten years. In the period, the compound dexamethasone acetate cream can be applied to a certain people hospital in Yueyang city to receive diagnosis and treatment due to intolerable pruritus and can be quickly relieved after the compound dexamethasone acetate cream is applied. The cerebral infarction is treated by being hospitalized with perineal eczema half year ago. During hospitalization, the western medicine hormone ointment treatment is still received, but the effect is not obvious, the traditional Chinese medicine rehabilitation therapy is recommended to be tried, and the skin oil is voluntarily tried. After the affected part is rinsed with the help of family people, a proper amount of the skin-generating oil (1) prepared in example 1 is applied to the perineum in a thin layer by using a clean cotton swab, the affected part is kept in a wet state for no less than 30 minutes after each application, and the skin-generating oil is applied for 1 time in the morning and at night every day for 5 days as a treatment course and is adhered continuously until the affected part is healed. The affected area was examined daily and recorded by photography.

(2) Therapeutic results

After the patient uses the skin-generating oil (figure 7), the pruritus at the perineum is obviously relieved, and the multiform red lesion and inflammatory exudation at the affected part are quickly inhibited. The family members refer to: after being applied for 10 days, the patient basically does not scratch the affected part of the perineum, does not have effusion, and the color of the affected part is close to normal. As the new years of life of many people are high and enuresis is frequent, family members are recommended to apply skin oil for at least 1 time every day to prevent eczema recurrence.

Example 8 skin oil preparation prepared in example 1 (i.e., topical skin oil (1)) for treating eczema on the cheek

(1) Method of treatment

For a 40 year old woman, the first two days are suddenly (for unknown reasons) soaked in water and itchy on the right cheek, and the affected part skin is scratched when the affected part skin is hard to endure. Then, the doctor goes to the X hospital dermatology department (the special important department in Wuhan City, the special combined traditional Chinese and Western medicine in China and one of the four Strength' departments in China) to ask for a diagnosis, and the doctor is diagnosed as the acute eczema by a certain chief and a certain medical doctor in famous physicians, and the following medicines are prescribed for oral administration and external use to cure the acute eczema:

1 triamcinolone acetonide econazole cream (10 g/bottle), 2 boxes of olopatadine hydrochloride tablets (10 tablets/box), 2 boxes of grass tablet (36 tablets/box), 15 g/bottle of geranium ointment and 150 ml/bottle of licorice Lin lotion.

After the medicine is used for two days according to the advice of doctors, the signs of redness, swelling, itching and pain of the cheek skin, blisters, exudation and the like are not obviously reduced. Then, the skin oil is voluntarily tried to be externally applied, and the internal and external medicines prescribed by the hospital are automatically stopped. Before applying the skin-generating oil externally, the affected part of the cheek is firstly moistened and washed with clear water, after air drying, a proper amount of the skin-generating oil (1) prepared in the example 1 is taken out by a clean cotton swab, and a thin layer is coated (actually, the skin-generating oil is coated for 1 time before sleep, and the affected part of the cheek faces upwards and sleeps for one night, so that the skin-generating oil is kept from being coated).

(2) Therapeutic results

The next day after the application of the skin oil (1) by the von lady (i.e. after 10 hours of application; fig. 8), the affected part of the cheek was first consciously touched without itching and pain; the affected area was found to be pale red when viewed in the mirror, and the swelling was essentially resolved, and the blisters disappeared with only a small amount of crust (right panel). It is recommended to stick on the surface for one or two days to consolidate the effect.

Claims

1. The external skin-generating oil for preventing and treating eczema and/or pruritus is characterized by being prepared from the following raw materials in parts by weight:

30-40 parts of astragalus membranaceus, 30-40 parts of angelica sinensis, 30-40 parts of gynura procumbens, 30-40 parts of garden burnet, 15-20 parts of frankincense, 15-20 parts of bletilla striata, 15-20 parts of angelica dahurica, 15-20 parts of cynanchum atratum, 10-20 parts of myrrh, 10-20 parts of scutellaria baicalensis, 10-20 parts of coptis chinensis, 10-20 parts of golden cypress, 10-20 parts of rheum officinale, 3-5 parts of borneol and 1000-1500 parts of edible vegetable oil.

2. The external skin oil for preventing and treating eczema and/or pruritus as claimed in claim 1, which is prepared from the following raw materials in parts by weight:

40 parts of astragalus membranaceus, 40 parts of angelica sinensis, 40 parts of gynura procumbens, 40 parts of garden burnet, 20 parts of frankincense, 20 parts of bletilla striata, 20 parts of angelica dahurica, 20 parts of cynanchum atratum, 20 parts of myrrh, 10 parts of scutellaria baicalensis, 10 parts of coptis chinensis, 10 parts of golden cypress, 10 parts of rheum officinale, 5 parts of borneol and 1500 parts of edible vegetable oil.

3. The external skin oil for preventing and treating eczema and/or pruritus as claimed in claim 2, which is prepared from the following raw materials in parts by weight:

30 parts of astragalus membranaceus, 30 parts of angelica sinensis, 30 parts of gynura procumbens, 30 parts of garden burnet, 15 parts of frankincense, 15 parts of bletilla striata, 15 parts of angelica dahurica, 15 parts of cynanchum atratum, 10 parts of myrrh, 20 parts of scutellaria baicalensis, 20 parts of coptis chinensis, 20 parts of phellodendron amurense, 20 parts of rheum officinale, 3 parts of borneol and 1185 parts of edible vegetable oil.

4. The external skin-generating oil for preventing and treating eczema and/or pruritus as claimed in any one of claims 1-3, wherein the preparation method of the external skin-generating oil comprises the following steps: putting edible vegetable oil into a container, and adding dry powder of radix astragali, radix Angelicae sinensis, radix Gynurae Divaricatae, radix Sangusorbae, rhizoma Bletillae, radix Angelicae Dahuricae, radix Cynanchi Atrati, scutellariae radix, radix et rhizoma Rhei, coptidis rhizoma, cortex Phellodendri, olibanum, and Myrrha; placing the container containing the above medicinal materials in a colloid mill, and circularly grinding at room temperature for 2-5h; and (3) after stopping, carrying out high-speed shearing, carrying out negative pressure sieving with a 5000-mesh sieve, removing slag, collecting filtered oil, adding borneol into the filtered oil, fully stirring, and cooling to obtain the external skin-generating oil.

5. The external skin oil for preventing and treating eczema and/or pruritus as claimed in claim 4, wherein the edible vegetable oil is at least one selected from sesame oil, peanut oil, coconut oil, soybean oil, linseed oil, castor oil and olive oil.

6. The external skin oil for preventing and treating eczema and/or pruritus as claimed in claim 4, wherein the high speed shearing step comprises: shearing with flash extraction device at 20000rpm for 5min 3-5 times.

7. Use of the topical skin oil of any one of claims 1-6 in the manufacture of a medicament for the prevention and treatment of eczema and/or pruritus.

8. Use according to claim 7, wherein the eczema and/or itching is caused by sensitising and/or inflammatory drugs.

9. Use according to claim 7, wherein the eczema is an acute eczema of the cheek or a chronic eczema of the perineum.